Rorug06G0417500

EamA-like transporter family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0014745
Physical Location & Seq
Forward (+)
55676409 .. 55677628
1220 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0417500.1

Sequence Viewer

Length: 843 bp
ATGGATTCACAGAGACTCGTCTTCGACAGTGTCAAGGCCGAGAAAGCCAATGCCATGAGACATTACCGGTGGCGCCAGAACCTCCGGTGGTTGTGGAAGGCGTGTCTGCTAGTCGCGCCGCTCTCTTACGTGCTCTGCCGCGACGCTCCCCGCCAACTCCTTATCGCCGTTTTCAACAGCAACCTCTGCGTTTTTTTGCTTCTTAATGCCTTGGTTGTCGTCACGTGTGTTTTGTCCGGCGAAAACGTCGCCGGCAGCGACAAGTCCGGAGGAGTTACGGAGAACGATATTTACGGCGAGTACGTTGCCAACGTGGAGTCTACTCGCCGGAGTGACCCTGTCGCCGGATTCAAGGGGTCTGAGTTGGTCAATCCCGAGGCGGAGGAGGGTTTAGCGAAGGAGAAAGAAATTGGATGTTTCGTTTCTCACCGGAGTATGGTCGCCGGATATCAGTCTCCGGCGGAAACTGAGACGGCTATTTCGCCGGAGGAAGTTTCAAACTTCCAAGGAACCGAATCCTATGAGAATGTGAATGTGGAATCTCTACTGCCGGTTTGTGAAGACAAAGCGGTTACTGAATGTAAAATTGTGGTCTCTTCAGTTTCTGCAGTTACTACGAGTACTAGTGGTTCTAGTGGTTGTGGTGAGGAAAGCGAAAAGGTGAAGGGTTACCGGAGGACACAATCGGAGAGTTTCGAGAAGAAGAGGAAGGAGCCTAAGAGGGGTTTGCAGCGATCGAAAACAAAGATGGAGGACTTGTCGGCTGAGGAGTTCCAACGCCAGATCGAGCTTTTCATTACTGCTAATAAGTTCATGATTCAGATGCTTGAGGAAGATAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EamA

Protein Analysis

280

Amino Acids

31.18

Weight (kDa)

5.31

Isoelectric Point (pI)

63.36

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014745)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g17290 FvH4_5g17290 FvH4_5g17290
malus_domestica MD06G1226600.v1.1
prunus_persica Prupe.5G232800_v2.0.a1 Prupe.5G232900_v2.0.a1
pyrus_communis pycom06g20290
rosa_chinensis RchiOBHm_Chr7g0178481
rosa_laevigata RLG00000005443
rosa_multiflora Rmu_sc0010235.1_g000003
rosa_roxburghii Rroxscaffold_3G00274860
rosa_rugosa Rorug06G0417500
rosa_samantha Rh7AG016000 Rh7BG015600 Rh7CG016300 Rh7DG016000
rosa_wichuraiana Rw7G001260

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 72
AccBSI CCGCTC 1 cut(s) 121
AccI GTMKAC 1 cut(s) 320
AccII CGCG 2 cut(s) 116, 141
AccIII TCCGGA 1 cut(s) 266
AciI CCGC 6 cut(s) 119, 139, 151, 380, 461, 569
AcuI CTGAAG 1 cut(s) 582
AcvI CACGTG 1 cut(s) 225
AcyI GRCGYC 1 cut(s) 73
AfaI GTAC 2 cut(s) 302, 622
AfiI CCNNNNNNNGG 3 cut(s) 344, 436, 722
AflIII ACRYGT 1 cut(s) 224
AgeI ACCGGT 1 cut(s) 66
AgsI TTSAA 3 cut(s) 175, 352, 498
AhlI ACTAGT 1 cut(s) 623
AluBI AGCT 1 cut(s) 790
AluI AGCT 1 cut(s) 790
Alw21I GWGCWC 1 cut(s) 135
Alw26I GTCTC 5 cut(s) 7, 52, 459, 464, 598
AlwNI CAGNNNCTG 1 cut(s) 605
Ama87I CYCGRG 1 cut(s) 374
Aor13HI TCCGGA 1 cut(s) 266
AoxI GGCC 1 cut(s) 36
ApeKI GCWGC 2 cut(s) 255, 730
ArsI GACNNNNNNTTYG 2 cut(s) 463, 495
AsiGI ACCGGT 1 cut(s) 66
AspLEI GCGC 2 cut(s) 75, 118
AsuHPI GGTGA 3 cut(s) 419, 656, 673
AvaI CYCGRG 1 cut(s) 374
BanI GGYRCC 1 cut(s) 72
BbrPI CACGTG 1 cut(s) 225
BbsI GAAGAC 2 cut(s) 13, 567
Bbv12I GWGCWC 1 cut(s) 135
BbvCI CCTCAGC 1 cut(s) 765
BbvI GCAGC 2 cut(s) 267, 742
BccI CCATC 1 cut(s) 742
BceAI ACGGC 3 cut(s) 152, 310, 489
BcgI CGANNNNNNTGC 2 cut(s) 287, 321
BcoDI GTCTC 5 cut(s) 7, 52, 459, 464, 598
BcuI ACTAGT 1 cut(s) 623
BfaI CTAG 3 cut(s) 110, 624, 633
BfmI CTRYAG 1 cut(s) 606
BfoI RGCGCY 1 cut(s) 76
BisI GCNGC 4 cut(s) 119, 139, 256, 731
BlsI GCNGC 4 cut(s) 120, 140, 257, 732
BmcAI AGTACT 1 cut(s) 622
BmeT110I CYCGRG 1 cut(s) 374
BmiI GGNNCC 3 cut(s) 74, 511, 714
BmsI GCATC 1 cut(s) 813
BpiI GAAGAC 2 cut(s) 13, 567
Bpu10I CCTNAGC 1 cut(s) 765
BsaAI YACGTR 2 cut(s) 130, 225
BsaHI GRCGYC 1 cut(s) 73
BsaI GGTCTC 1 cut(s) 598
BsaJI CCNNGG 3 cut(s) 210, 375, 505
BsaWI WCCGGW 5 cut(s) 66, 84, 266, 429, 672
BsaXI ACNNNNNCTCC 2 cut(s) 743, 773
Bsc4I CCNNNNNNNGG 3 cut(s) 344, 436, 722
Bse118I RCCGGY 3 cut(s) 66, 251, 550
BseAI TCCGGA 1 cut(s) 266
BseDI CCNNGG 3 cut(s) 210, 375, 505
BseGI GGATG 1 cut(s) 419
BseLI CCNNNNNNNGG 3 cut(s) 344, 436, 722
BseMII CTCAG 3 cut(s) 351, 459, 756
BseRI GAGGAG 3 cut(s) 285, 398, 782
BseXI GCAGC 2 cut(s) 267, 742
Bsh1236I CGCG 2 cut(s) 116, 141
Bsh1285I CGRYCG 1 cut(s) 737
BshFI GGCC 1 cut(s) 38
BshNI GGYRCC 1 cut(s) 72
BshTI ACCGGT 1 cut(s) 66
BsiEI CGRYCG 1 cut(s) 737
BsiHKAI GWGCWC 1 cut(s) 135
BsiHKCI CYCGRG 1 cut(s) 374
BslI CCNNNNNNNGG 3 cut(s) 344, 436, 722
BsmAI GTCTC 5 cut(s) 7, 52, 459, 464, 598
BsmBI CGTCTC 1 cut(s) 464
BsnI GGCC 1 cut(s) 38
Bso31I GGTCTC 1 cut(s) 598
BsoBI CYCGRG 1 cut(s) 374
Bsp1286I GDGCHC 1 cut(s) 135
Bsp13I TCCGGA 1 cut(s) 266
Bsp143I GATC 2 cut(s) 734, 783
BspACI CCGC 6 cut(s) 119, 139, 151, 380, 461, 569
BspANI GGCC 1 cut(s) 38
BspCNI CTCAG 3 cut(s) 352, 460, 757
BspEI TCCGGA 1 cut(s) 266
BspFNI CGCG 2 cut(s) 116, 141
BspHI TCATGA 1 cut(s) 813
BspLI GGNNCC 3 cut(s) 74, 511, 714
BspMAI CTGCAG 1 cut(s) 610
BspT107I GGYRCC 1 cut(s) 72
BspTNI GGTCTC 1 cut(s) 598
BsrBI CCGCTC 1 cut(s) 121
BsrFI RCCGGY 3 cut(s) 66, 251, 550
BssAI RCCGGY 3 cut(s) 66, 251, 550
BssECI CCNNGG 3 cut(s) 210, 375, 505
BssMI GATC 2 cut(s) 734, 783
BssNI GRCGYC 1 cut(s) 73
BssT1I CCWWGG 2 cut(s) 210, 505
Bst4CI ACNGT 1 cut(s) 29
Bst6I CTCTTC 2 cut(s) 601, 698
BstACI GRCGYC 1 cut(s) 73
BstAPI GCANNNNNTGC 1 cut(s) 186
BstBAI YACGTR 2 cut(s) 130, 225
BstC8I GCNNGC 1 cut(s) 253
BstDEI CTNAG 4 cut(s) 360, 468, 717, 765
BstEII GGTNACC 1 cut(s) 668
BstF5I GGATG 1 cut(s) 419
BstFNI CGCG 2 cut(s) 116, 141
BstH2I RGCGCY 1 cut(s) 76
BstHHI GCGC 2 cut(s) 75, 118
BstKTI GATC 2 cut(s) 737, 786
BstMAI GTCTC 5 cut(s) 7, 52, 459, 464, 598
BstMBI GATC 2 cut(s) 734, 783
BstMCI CGRYCG 1 cut(s) 737
BstMWI GCNNNNNNNGC 3 cut(s) 44, 115, 186
BstPI GGTNACC 1 cut(s) 668
BstSFI CTRYAG 1 cut(s) 606
BstUI CGCG 2 cut(s) 116, 141
BstV1I GCAGC 2 cut(s) 267, 742
BstV2I GAAGAC 2 cut(s) 13, 567
BsuRI GGCC 1 cut(s) 38
BtsCI GGATG 1 cut(s) 419
BtsIMutI CAGTG 1 cut(s) 34
Cac8I GCNNGC 1 cut(s) 253
CaiI CAGNNNCTG 1 cut(s) 605
CciI TCATGA 1 cut(s) 813
CfoI GCGC 2 cut(s) 75, 118
Cfr10I RCCGGY 3 cut(s) 66, 251, 550
CseI GACGC 1 cut(s) 152
Csp6I GTAC 2 cut(s) 301, 621
CspAI ACCGGT 1 cut(s) 66
CviAII CATG 2 cut(s) 55, 814
CviJI RGCY 6 cut(s) 38, 47, 476, 715, 764, 790
CviKI_1 RGCY 6 cut(s) 38, 47, 476, 715, 764, 790
CviQI GTAC 2 cut(s) 301, 621
DdeI CTNAG 4 cut(s) 360, 468, 717, 765
DinI GGCGCC 1 cut(s) 74
DpnI GATC 2 cut(s) 736, 785
DpnII GATC 2 cut(s) 734, 783
Eam1104I CTCTTC 2 cut(s) 601, 698
EarI CTCTTC 2 cut(s) 601, 698
EciI GGCGGA 2 cut(s) 395, 476
Eco130I CCWWGG 2 cut(s) 210, 505
Eco31I GGTCTC 1 cut(s) 598
Eco32I GATATC 1 cut(s) 449
Eco57I CTGAAG 1 cut(s) 582
Eco72I CACGTG 1 cut(s) 225
Eco88I CYCGRG 1 cut(s) 374
Eco91I GGTNACC 1 cut(s) 668
EcoO65I GGTNACC 1 cut(s) 668
EcoRV GATATC 1 cut(s) 449
EcoT14I CCWWGG 2 cut(s) 210, 505
EgeI GGCGCC 1 cut(s) 74
EheI GGCGCC 1 cut(s) 74
ErhI CCWWGG 2 cut(s) 210, 505
Esp3I CGTCTC 1 cut(s) 464
FaeI CATG 2 cut(s) 58, 817
FaiI YATR 4 cut(s) 56, 437, 522, 815
FatI CATG 2 cut(s) 54, 813
FauI CCCGC 1 cut(s) 158
FblI GTMKAC 1 cut(s) 320
Fnu4HI GCNGC 4 cut(s) 119, 139, 256, 731
FokI GGATG 1 cut(s) 426
Fsp4HI GCNGC 4 cut(s) 119, 139, 256, 731
FspBI CTAG 3 cut(s) 110, 624, 633
GlaI GCGC 2 cut(s) 74, 117
GluI GCNGC 4 cut(s) 119, 139, 256, 731
HaeII RGCGCY 1 cut(s) 76
HaeIII GGCC 1 cut(s) 38
HgaI GACGC 1 cut(s) 152
HhaI GCGC 2 cut(s) 75, 118
Hin1I GRCGYC 1 cut(s) 73
Hin1II CATG 2 cut(s) 58, 817
Hin6I GCGC 2 cut(s) 73, 116
HinP1I GCGC 2 cut(s) 73, 116
HinfI GANTC 7 cut(s) 5, 15, 317, 348, 515, 539, 817
HphI GGTGA 3 cut(s) 419, 656, 673
Hpy166II GTNNAC 1 cut(s) 321
Hpy188I TCNGA 3 cut(s) 361, 688, 822
Hpy188III TCNNGA 4 cut(s) 267, 374, 697, 814
Hpy8I GTNNAC 1 cut(s) 321
Hpy99I CGWCG 2 cut(s) 146, 251
HpyAV CCTTC 4 cut(s) 91, 391, 658, 703
HpyCH4III ACNGT 1 cut(s) 29
HpyCH4IV ACGT 5 cut(s) 129, 224, 246, 303, 312
HpyCH4V TGCA 2 cut(s) 608, 730
HpyF10VI GCNNNNNNNGC 3 cut(s) 44, 115, 186
HpyF3I CTNAG 4 cut(s) 360, 468, 717, 765
HpySE526I ACGT 5 cut(s) 129, 224, 246, 303, 312
Hsp92I GRCGYC 1 cut(s) 73
Hsp92II CATG 2 cut(s) 58, 817
HspAI GCGC 2 cut(s) 73, 116
KasI GGCGCC 1 cut(s) 72
Kpn2I TCCGGA 1 cut(s) 266
KroI GCCGGC 1 cut(s) 251
KroNI GCCGGC 1 cut(s) 253
Kzo9I GATC 2 cut(s) 734, 783
LmnI GCTCC 2 cut(s) 151, 712
Lsp1109I GCAGC 2 cut(s) 267, 742
LweI GCATC 1 cut(s) 813
MaeI CTAG 3 cut(s) 110, 624, 633
MaeII ACGT 5 cut(s) 129, 224, 246, 303, 312
MaeIII GTNAC 6 cut(s) 220, 274, 332, 571, 610, 668
MalI GATC 2 cut(s) 736, 785
MbiI CCGCTC 1 cut(s) 121
MboI GATC 2 cut(s) 734, 783
MboII GAAGA 5 cut(s) 13, 572, 588, 712, 715
MhlI GDGCHC 1 cut(s) 135
MluCI AATT 2 cut(s) 408, 585
Mly113I GGCGCC 1 cut(s) 73
MlyI GAGTC 2 cut(s) 9, 326
MmeI TCCRAC 1 cut(s) 799
MroI TCCGGA 1 cut(s) 266
MroNI GCCGGC 1 cut(s) 251
MseI TTAA 1 cut(s) 204
MvnI CGCG 2 cut(s) 116, 141
MwoI GCNNNNNNNGC 3 cut(s) 44, 115, 186
NaeI GCCGGC 1 cut(s) 253
NarI GGCGCC 1 cut(s) 73
NdeII GATC 2 cut(s) 734, 783
NgoMIV GCCGGC 1 cut(s) 251
NlaIII CATG 2 cut(s) 58, 817
NlaIV GGNNCC 3 cut(s) 74, 511, 714
NmeAIII GCCGAG 1 cut(s) 64
NmuCI GTSAC 2 cut(s) 220, 332
PagI TCATGA 1 cut(s) 813
PcsI WCGNNNNNNNCGW 3 cut(s) 255, 300, 309
PdiI GCCGGC 1 cut(s) 253
PfeI GAWTC 5 cut(s) 5, 348, 515, 539, 817
PflFI GACNNNGTC 2 cut(s) 29, 338
PinAI ACCGGT 1 cut(s) 66
PkrI GCNGC 4 cut(s) 120, 140, 257, 732
Ple19I CGATCG 1 cut(s) 737
PleI GAGTC 2 cut(s) 9, 325
PluTI GGCGCC 1 cut(s) 76
PmaCI CACGTG 1 cut(s) 225
PmlI CACGTG 1 cut(s) 225
PpsI GAGTC 2 cut(s) 9, 325
Ppu21I YACGTR 2 cut(s) 130, 225
PspCI CACGTG 1 cut(s) 225
PspEI GGTNACC 1 cut(s) 668
PspN4I GGNNCC 3 cut(s) 74, 511, 714
PsrI GAACNNNNNNTAC 4 cut(s) 275, 307, 613, 645
PstI CTGCAG 1 cut(s) 610
PstNI CAGNNNCTG 1 cut(s) 605
PsyI GACNNNGTC 2 cut(s) 29, 338
PvuI CGATCG 1 cut(s) 737
RsaI GTAC 2 cut(s) 302, 622
RsaNI GTAC 2 cut(s) 301, 621
SaqAI TTAA 1 cut(s) 204
SatI GCNGC 4 cut(s) 119, 139, 256, 731
Sau3AI GATC 2 cut(s) 734, 783
ScaI AGTACT 1 cut(s) 622
SchI GAGTC 2 cut(s) 9, 326
SduI GDGCHC 1 cut(s) 135
SetI ASST 9 cut(s) 84, 132, 186, 227, 249, 306, 315, 663, 792
SfaNI GCATC 1 cut(s) 813
SfcI CTRYAG 1 cut(s) 606
SfoI GGCGCC 1 cut(s) 74
SmlI CTYRAG 1 cut(s) 827
SmoI CTYRAG 1 cut(s) 827
SpeI ACTAGT 1 cut(s) 623
Sse9I AATT 2 cut(s) 408, 585
SsiI CCGC 6 cut(s) 119, 139, 151, 380, 461, 569
SspDI GGCGCC 1 cut(s) 72
SspMI CTAG 3 cut(s) 110, 624, 633
StyI CCWWGG 2 cut(s) 210, 505
TaaI ACNGT 1 cut(s) 29
TaiI ACGT 5 cut(s) 132, 227, 249, 306, 315
TaqI TCGA 4 cut(s) 24, 696, 737, 786
TasI AATT 2 cut(s) 408, 585
TatI WGTACW 1 cut(s) 620
TauI GCSGC 2 cut(s) 121, 141
TfiI GAWTC 5 cut(s) 5, 348, 515, 539, 817
Tru1I TTAA 1 cut(s) 204
Tru9I TTAA 1 cut(s) 204
TscAI CASTG 1 cut(s) 34
TseFI GTSAC 2 cut(s) 220, 332
TseI GCWGC 2 cut(s) 255, 730
Tsp45I GTSAC 2 cut(s) 220, 332
TspDTI ATGAA 2 cut(s) 784, 802
TspGWI ACGGA 1 cut(s) 293
TspRI CASTG 1 cut(s) 34
Tth111I GACNNNGTC 2 cut(s) 29, 338
XmiI GTMKAC 1 cut(s) 320
XspI CTAG 3 cut(s) 110, 624, 633
ZrmI AGTACT 1 cut(s) 622
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.