Rmu_sc0010235.1_g000003

EamA-like transporter family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0010235.1
OG0014745
Physical Location & Seq
Forward (+)
11694 .. 14141
2448 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0010235.1_g000003.1.cds

Sequence Viewer

Length: 1335 bp
atgttcctcttcttttccactaccattcccaacaaacggcggcgtctaatcgcttgttcattagatcccatctccacttcatcactaacatctcagaatgctactcacaagaacagaacagacaagaaatctgttgaaattcaaagaaccaatgatgggactttctctgttttacacagtgatgagcacaaaccatatgagaagaagatcaagaacagcaagaaggatgaagctaataaggggttgaaatctggttccaaaaagcgacgacctttgtggcaaaaggctttctttgcatccaagaagatgaggagtattttgttgctcaatgcaatcacaattgtctatgcaagtgacattccagttattaaagaagttgaaactatcatggacccagcagccttctcagcggtgcggtttatcatgtcagcaattccatttctgccattcgtgtttcgatcaccgggtgatgttcagacccgaaatgcagggattgagttggggttctggatcagtctaggctaccttcttgaggcacttggtttacttacatctgatgctggccgtgcatcgtttatttccctcttcacagttattgtggtacctttgcttgatggcttgtttggatcaatcatacctgctagatcctggttcggagttctcatgtctgctcttggggttggtatgctggaatgtagtggatcacctccaaatgttggagatctcttcaacttcttgagtgcaatatttttcggaattcacatgcttcgaactgagcatatttcaagaagcacaaagaaggagaacttcctagcaattcttggatatgaggtcagtgttgttgctgtcttatccacaatctgggtttttattggagggtggtttgatggtgcccaaagttccaaccaattaacatggacgtggacagagatgtgggattggcttgttgcatttccatggatacctgcactttatactggtgcattttcaactgggatatgcttatggattgagattgcagcgatgcgcgatgtttcagcaacagaaacagccataatttatgggctggagccactgtggggagctggatttgcatggtttcttcttggtgaaagatggggagcacttggatggatcggtgcagctcttgtgcttggtggaagcctagtggtgcagatatttgggtcctcaacttcaaaagaatttatagtttctgaagagagcaaggagaaaggtgatctcctaagggttccagttgaccaaaaattacaaaatagtctctctacttctccattagttgtcaggtccaaaaaggatctaacagattttctatag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EamA

Protein Analysis

444

Amino Acids

49.15

Weight (kDa)

9.07

Isoelectric Point (pI)

43.88

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014745)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g17290 FvH4_5g17290 FvH4_5g17290
malus_domestica MD06G1226600.v1.1
prunus_persica Prupe.5G232800_v2.0.a1 Prupe.5G232900_v2.0.a1
pyrus_communis pycom06g20290
rosa_chinensis RchiOBHm_Chr7g0178481
rosa_laevigata RLG00000005443
rosa_multiflora Rmu_sc0010235.1_g000003
rosa_roxburghii Rroxscaffold_3G00274860
rosa_rugosa Rorug06G0417500
rosa_samantha Rh7AG016000 Rh7BG015600 Rh7CG016300 Rh7DG016000
rosa_wichuraiana Rw7G001260

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 646, 973
Acc65I GGTACC 1 cut(s) 601
AccB1I GGYRCC 2 cut(s) 601, 890
AccB7I CCANNNNNTGG 2 cut(s) 716, 861
AccII CGCG 1 cut(s) 1029
AciI CCGC 3 cut(s) 40, 410, 415
AclWI GGATC 7 cut(s) 59, 518, 634, 639, 709, 1142, 1323
AcoI YGGCCR 1 cut(s) 562
AcsI RAATTY 3 cut(s) 138, 756, 1202
AcuI CTGAAG 1 cut(s) 1236
AcyI GRCGYC 1 cut(s) 43
AdeI CACNNNGTG 1 cut(s) 467
AfaI GTAC 1 cut(s) 603
AfiI CCNNNNNNNGG 6 cut(s) 36, 156, 532, 716, 861, 1079
AgsI TTSAA 8 cut(s) 137, 143, 247, 380, 730, 786, 990, 1197
AjiI CACGTC 1 cut(s) 921
AjnI CCWGG 1 cut(s) 647
AluBI AGCT 3 cut(s) 233, 1085, 1145
AluI AGCT 3 cut(s) 233, 1085, 1145
Alw21I GWGCWC 2 cut(s) 189, 1126
Alw26I GTCTC 1 cut(s) 1283
AlwI GGATC 7 cut(s) 59, 518, 634, 639, 709, 1142, 1323
AoxI GGCC 1 cut(s) 562
ApeKI GCWGC 3 cut(s) 398, 1019, 1142
ApoI RAATTY 3 cut(s) 138, 756, 1202
Asp718I GGTACC 1 cut(s) 601
AspLEI GCGC 1 cut(s) 1029
AspS9I GGNCC 3 cut(s) 391, 1185, 1305
AsuC2I CCSGG 1 cut(s) 465
AsuHPI GGTGA 5 cut(s) 453, 479, 696, 1121, 1247
AsuII TTCGAA 1 cut(s) 769
AvaII GGWCC 3 cut(s) 391, 1185, 1305
AxyI CCTNAGG 1 cut(s) 1244
BaeGI GKGCMC 1 cut(s) 895
BanI GGYRCC 2 cut(s) 601, 890
Bbv12I GWGCWC 2 cut(s) 189, 1126
BbvI GCAGC 3 cut(s) 410, 1031, 1154
BccI CCATC 6 cut(s) 77, 149, 608, 881, 1110, 1125
BceAI ACGGC 2 cut(s) 53, 549
BciT130I CCWGG 1 cut(s) 649
BciVI GTATCC 1 cut(s) 954
BcnI CCSGG 1 cut(s) 465
BcoDI GTCTC 1 cut(s) 1283
BfaI CTAG 4 cut(s) 518, 642, 812, 1166
BfmI CTRYAG 1 cut(s) 1331
BfuAI ACCTGC 2 cut(s) 646, 973
BfuI GTATCC 1 cut(s) 954
BglII AGATCT 1 cut(s) 721
BisI GCNGC 4 cut(s) 41, 399, 1020, 1143
BlsI GCNGC 4 cut(s) 42, 400, 1021, 1144
Bme1390I CCNGG 2 cut(s) 465, 649
Bme18I GGWCC 3 cut(s) 391, 1185, 1305
BmgBI CACGTC 1 cut(s) 921
BmgT120I GGNCC 3 cut(s) 391, 1185, 1305
BmiI GGNNCC 7 cut(s) 256, 393, 603, 892, 1071, 1186, 1251
BmrFI CCNGG 2 cut(s) 465, 649
BmrI ACTGGG 1 cut(s) 1002
BmsI GCATC 4 cut(s) 305, 547, 578, 1014
BmuI ACTGGG 1 cut(s) 1002
BpmI CTGGAG 1 cut(s) 1088
Bpu14I TTCGAA 1 cut(s) 769
BpuEI CTTGAG 2 cut(s) 551, 757
BpuMI CCSGG 1 cut(s) 465
BsaHI GRCGYC 1 cut(s) 43
BsaJI CCNNGG 1 cut(s) 956
BsaXI ACNNNNNCTCC 2 cut(s) 1061, 1091
Bsc4I CCNNNNNNNGG 6 cut(s) 36, 156, 532, 716, 861, 1079
Bse1I ACTGG 4 cut(s) 362, 982, 997, 1253
Bse21I CCTNAGG 1 cut(s) 1244
BseBI CCWGG 1 cut(s) 649
BseDI CCNNGG 1 cut(s) 956
BseGI GGATG 3 cut(s) 232, 296, 1136
BseLI CCNNNNNNNGG 6 cut(s) 36, 156, 532, 716, 861, 1079
BseMII CTCAG 3 cut(s) 107, 420, 765
BseNI ACTGG 4 cut(s) 362, 982, 997, 1253
BseRI GAGGAG 1 cut(s) 325
BseSI GKGCMC 1 cut(s) 895
BseXI GCAGC 3 cut(s) 410, 1031, 1154
BseYI CCCAGC 1 cut(s) 394
BsgI GTGCAG 3 cut(s) 951, 1161, 1193
Bsh1236I CGCG 1 cut(s) 1029
BshFI GGCC 1 cut(s) 564
BshNI GGYRCC 2 cut(s) 601, 890
BsiHKAI GWGCWC 2 cut(s) 189, 1126
BsiSI CCGG 1 cut(s) 464
BslFI GGGAC 1 cut(s) 172
BslI CCNNNNNNNGG 6 cut(s) 36, 156, 532, 716, 861, 1079
BsmAI GTCTC 1 cut(s) 1283
BsmFI GGGAC 1 cut(s) 172
BsmI GAATGC 1 cut(s) 103
BsnI GGCC 1 cut(s) 564
Bsp119I TTCGAA 1 cut(s) 769
Bsp1286I GDGCHC 3 cut(s) 189, 895, 1126
Bsp19I CCATGG 1 cut(s) 956
BspACI CCGC 3 cut(s) 40, 410, 415
BspANI GGCC 1 cut(s) 564
BspCNI CTCAG 3 cut(s) 106, 419, 766
BspFNI CGCG 1 cut(s) 1029
BspLI GGNNCC 7 cut(s) 256, 393, 603, 892, 1071, 1186, 1251
BspMI ACCTGC 2 cut(s) 646, 973
BspPI GGATC 7 cut(s) 59, 518, 634, 639, 709, 1142, 1323
BspT104I TTCGAA 1 cut(s) 769
BspT107I GGYRCC 2 cut(s) 601, 890
BsrI ACTGG 4 cut(s) 362, 982, 997, 1253
BssECI CCNNGG 1 cut(s) 956
BssNI GRCGYC 1 cut(s) 43
BssT1I CCWWGG 1 cut(s) 956
Bst2UI CCWGG 1 cut(s) 649
Bst4CI ACNGT 3 cut(s) 179, 592, 1077
Bst6I CTCTTC 4 cut(s) 14, 590, 731, 1212
BstACI GRCGYC 1 cut(s) 43
BstBI TTCGAA 1 cut(s) 769
BstC8I GCNNGC 1 cut(s) 562
BstDEI CTNAG 4 cut(s) 93, 406, 774, 1244
BstDSI CCRYGG 1 cut(s) 956
BstENI CCTNNNNNAGG 1 cut(s) 530
BstF5I GGATG 3 cut(s) 232, 296, 1136
BstFNI CGCG 1 cut(s) 1029
BstHHI GCGC 1 cut(s) 1029
BstMAI GTCTC 1 cut(s) 1283
BstMWI GCNNNNNNNGC 4 cut(s) 293, 407, 566, 1091
BstNI CCWGG 1 cut(s) 649
BstNSI RCATGY 1 cut(s) 766
BstSCI CCNGG 2 cut(s) 463, 647
BstSFI CTRYAG 1 cut(s) 1331
BstSLI GKGCMC 1 cut(s) 895
BstUI CGCG 1 cut(s) 1029
BstV1I GCAGC 3 cut(s) 410, 1031, 1154
BstX2I RGATCY 4 cut(s) 64, 644, 721, 1315
BstYI RGATCY 4 cut(s) 64, 644, 721, 1315
Bsu36I CCTNAGG 1 cut(s) 1244
BsuI GTATCC 1 cut(s) 954
BsuRI GGCC 1 cut(s) 564
BtgI CCRYGG 1 cut(s) 956
BtgZI GCGATG 2 cut(s) 1037, 1044
BtrI CACGTC 1 cut(s) 921
BtsCI GGATG 3 cut(s) 232, 296, 1136
BtsIMutI CAGTG 3 cut(s) 184, 841, 1073
BveI ACCTGC 2 cut(s) 646, 973
Cac8I GCNNGC 1 cut(s) 562
CfoI GCGC 1 cut(s) 1029
Cfr13I GGNCC 3 cut(s) 391, 1185, 1305
CseI GACGC 1 cut(s) 32
Csp6I GTAC 1 cut(s) 602
CviAII CATG 7 cut(s) 388, 424, 664, 763, 915, 957, 1095
CviQI GTAC 1 cut(s) 602
DdeI CTNAG 4 cut(s) 93, 406, 774, 1244
DraIII CACNNNGTG 1 cut(s) 467
EaeI YGGCCR 1 cut(s) 562
Eam1104I CTCTTC 4 cut(s) 14, 590, 731, 1212
EarI CTCTTC 4 cut(s) 14, 590, 731, 1212
Eco130I CCWWGG 1 cut(s) 956
Eco47I GGWCC 3 cut(s) 391, 1185, 1305
Eco57I CTGAAG 1 cut(s) 1236
Eco81I CCTNAGG 1 cut(s) 1244
EcoNI CCTNNNNNAGG 1 cut(s) 530
EcoO109I RGGNCCY 1 cut(s) 1185
EcoRI GAATTC 1 cut(s) 756
EcoRII CCWGG 1 cut(s) 647
EcoT14I CCWWGG 1 cut(s) 956
ErhI CCWWGG 1 cut(s) 956
FaeI CATG 7 cut(s) 391, 427, 667, 766, 918, 960, 1098
FalI AAGNNNNNCTT 4 cut(s) 275, 307, 791, 823
FaqI GGGAC 1 cut(s) 172
FatI CATG 7 cut(s) 387, 423, 663, 762, 914, 956, 1094
FauNDI CATATG 1 cut(s) 196
Fnu4HI GCNGC 4 cut(s) 41, 399, 1020, 1143
FokI GGATG 3 cut(s) 239, 283, 1143
Fsp4HI GCNGC 4 cut(s) 41, 399, 1020, 1143
FspBI CTAG 4 cut(s) 518, 642, 812, 1166
GlaI GCGC 1 cut(s) 1028
GluI GCNGC 4 cut(s) 41, 399, 1020, 1143
GsaI CCCAGC 1 cut(s) 398
GsuI CTGGAG 1 cut(s) 1088
HaeIII GGCC 1 cut(s) 564
HapII CCGG 1 cut(s) 464
HgaI GACGC 1 cut(s) 32
HhaI GCGC 1 cut(s) 1029
Hin1I GRCGYC 1 cut(s) 43
Hin1II CATG 7 cut(s) 391, 427, 667, 766, 918, 960, 1098
Hin6I GCGC 1 cut(s) 1027
HinP1I GCGC 1 cut(s) 1027
HincII GTYRAC 1 cut(s) 1258
HindII GTYRAC 1 cut(s) 1258
HpaII CCGG 1 cut(s) 464
HphI GGTGA 5 cut(s) 453, 479, 696, 1121, 1247
Hpy166II GTNNAC 3 cut(s) 545, 924, 1258
Hpy188I TCNGA 6 cut(s) 96, 477, 556, 656, 755, 1216
Hpy188III TCNNGA 5 cut(s) 211, 508, 530, 736, 786
Hpy8I GTNNAC 3 cut(s) 545, 924, 1258
Hpy99I CGWCG 1 cut(s) 270
HpyAV CCTTC 4 cut(s) 217, 412, 536, 793
HpyCH4III ACNGT 3 cut(s) 179, 592, 1077
HpyCH4IV ACGT 1 cut(s) 920
HpyF10VI GCNNNNNNNGC 4 cut(s) 293, 407, 566, 1091
HpyF3I CTNAG 4 cut(s) 93, 406, 774, 1244
HpySE526I ACGT 1 cut(s) 920
Hsp92I GRCGYC 1 cut(s) 43
Hsp92II CATG 7 cut(s) 391, 427, 667, 766, 918, 960, 1098
HspAI GCGC 1 cut(s) 1027
KpnI GGTACC 1 cut(s) 605
LmnI GCTCC 3 cut(s) 1069, 1082, 1121
Lsp1109I GCAGC 3 cut(s) 410, 1031, 1154
LweI GCATC 4 cut(s) 305, 547, 578, 1014
MaeI CTAG 4 cut(s) 518, 642, 812, 1166
MaeII ACGT 1 cut(s) 920
MaeIII GTNAC 1 cut(s) 353
MboII GAAGA 7 cut(s) 214, 217, 316, 577, 718, 1094, 1229
MfeI CAATTG 1 cut(s) 339
MflI RGATCY 4 cut(s) 64, 644, 721, 1315
MhlI GDGCHC 3 cut(s) 189, 895, 1126
MluCI AATT 9 cut(s) 138, 339, 432, 756, 816, 908, 1056, 1202, 1265
MmeI TCCRAC 2 cut(s) 697, 927
MnlI CCTC 8 cut(s) 17, 303, 526, 593, 717, 823, 869, 1198
MseI TTAA 2 cut(s) 369, 911
MslI CAYNNNNRTG 4 cut(s) 180, 919, 955, 1129
MspA1I CMGCKG 1 cut(s) 410
MspI CCGG 1 cut(s) 464
MspR9I CCNGG 2 cut(s) 465, 649
MunI CAATTG 1 cut(s) 339
Mva1269I GAATGC 1 cut(s) 103
MvaI CCWGG 1 cut(s) 649
MvnI CGCG 1 cut(s) 1029
MwoI GCNNNNNNNGC 4 cut(s) 293, 407, 566, 1091
NciI CCSGG 1 cut(s) 465
NcoI CCATGG 1 cut(s) 956
NdeI CATATG 1 cut(s) 196
NlaIII CATG 7 cut(s) 391, 427, 667, 766, 918, 960, 1098
NlaIV GGNNCC 7 cut(s) 256, 393, 603, 892, 1071, 1186, 1251
NmuCI GTSAC 1 cut(s) 353
NspI RCATGY 1 cut(s) 766
NspV TTCGAA 1 cut(s) 769
PctI GAATGC 1 cut(s) 103
PflMI CCANNNNNTGG 2 cut(s) 716, 861
PkrI GCNGC 4 cut(s) 42, 400, 1021, 1144
PpuMI RGGWCCY 1 cut(s) 1185
Psp5II RGGWCCY 1 cut(s) 1185
Psp6I CCWGG 1 cut(s) 647
PspFI CCCAGC 1 cut(s) 394
PspGI CCWGG 1 cut(s) 647
PspN4I GGNNCC 7 cut(s) 256, 393, 603, 892, 1071, 1186, 1251
PspPI GGNCC 3 cut(s) 391, 1185, 1305
PspPPI RGGWCCY 1 cut(s) 1185
PsuI RGATCY 4 cut(s) 64, 644, 721, 1315
RsaI GTAC 1 cut(s) 603
RsaNI GTAC 1 cut(s) 602
RseI CAYNNNNRTG 4 cut(s) 180, 919, 955, 1129
SaqAI TTAA 2 cut(s) 369, 911
SatI GCNGC 4 cut(s) 41, 399, 1020, 1143
Sau96I GGNCC 3 cut(s) 391, 1185, 1305
ScrFI CCNGG 2 cut(s) 465, 649
SduI GDGCHC 3 cut(s) 189, 895, 1126
SfaNI GCATC 4 cut(s) 305, 547, 578, 1014
SfcI CTRYAG 1 cut(s) 1331
SfuI TTCGAA 1 cut(s) 769
SinI GGWCC 3 cut(s) 391, 1185, 1305
SmiMI CAYNNNNRTG 4 cut(s) 180, 919, 955, 1129
SmlI CTYRAG 2 cut(s) 530, 736
SmoI CTYRAG 2 cut(s) 530, 736
Sse9I AATT 9 cut(s) 138, 339, 432, 756, 816, 908, 1056, 1202, 1265
SsiI CCGC 3 cut(s) 40, 410, 415
SspI AATATT 1 cut(s) 747
SspMI CTAG 4 cut(s) 518, 642, 812, 1166
StyD4I CCNGG 2 cut(s) 463, 647
StyI CCWWGG 1 cut(s) 956
TaaI ACNGT 3 cut(s) 179, 592, 1077
TaiI ACGT 1 cut(s) 923
TaqI TCGA 2 cut(s) 457, 769
TasI AATT 9 cut(s) 138, 339, 432, 756, 816, 908, 1056, 1202, 1265
TauI GCSGC 1 cut(s) 43
Tru1I TTAA 2 cut(s) 369, 911
Tru9I TTAA 2 cut(s) 369, 911
TscAI CASTG 3 cut(s) 184, 841, 1080
TseFI GTSAC 1 cut(s) 353
TseI GCWGC 3 cut(s) 398, 1019, 1142
Tsp45I GTSAC 1 cut(s) 353
TspDTI ATGAA 3 cut(s) 48, 69, 243
TspRI CASTG 3 cut(s) 184, 841, 1080
Van91I CCANNNNNTGG 2 cut(s) 716, 861
VpaK11BI GGWCC 3 cut(s) 391, 1185, 1305
XagI CCTNNNNNAGG 1 cut(s) 530
XapI RAATTY 3 cut(s) 138, 756, 1202
XceI RCATGY 1 cut(s) 766
XspI CTAG 4 cut(s) 518, 642, 812, 1166
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.