Rw7G001260

EamA-like transporter family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0014745
Physical Location & Seq
Reverse (-)
1062713 .. 1065135
2423 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G001260.1

Sequence Viewer

Length: 1383 bp
ATGGCTTGTTCATCATCAAATATATGGAGGGTGACCACACTTGACTCTGGCTCAAAAATTTCAAGCTACTTTAGCAACCCTGCCTCCATGTTCCTCTTCTTTTCCACTACCATTCCCAACAAACGGCGGCGTCTAATCGCTTGTTCATTAGATCCCATCTCCACTTCATCACTAACATCTCAGAATGGTACTCACAAGAACAGAACAGACAAGAAATCTGTTGAAATTCAAAGAACCAATGATGGGACTTTCTCTGTTTTACACAGTGATGAGCACAAACCATATGAGAAGAAGATCAAGAACAGCAAGAAGGATGAAGCTAAGAATAAGGGGTTGAAATCTGGTTCCAAAAAGCGACGACCTTTGTGGCAAAAGGCTTTCTTTGCATCCAAGAAGATGAGGAGTATTTTGTTGCTCAATGCAATCACAATTGTCTATGCAAGTGACATTCCAGTTATTAAAGAAGTTGAAACTATCATGGACCCAGCAGCCTTCTCAGCGGTGCGGTTTATCATGTCAGCAATTCCATTTCTGCCATTCGTGTTTCGATCACCGGGTGATGTTCAGACCCGAAATGCAGGGATTGAGTTGGGGTTCTGGATCAGTCTAGGCTACCTTCTTGAGGCACTTGGTTTACTTACATCTGATGCTGGCCGTGCATCGTTTATTTCCCTCTTCACAGTTATTGTGGTACCTTTGCTTGATGGCTTGTTTGGATCAATCATACCTGCTAGATCCTGGTTCGGAGTTCTCATGTCTGCTCTTGGGGTTGGTATGCTGGAATGTAGTGGATCACCTCCAAATGTTGGAGATCTCTTCAACTTCTTGAGTGCAATATTTTTCGGAATTCACATGCTTCGAACTGAGCATATTTCAAGAAGCACAAAGAAGGAGAACTTCCTAGCAATTCTTGGATATGAGGTCAGTGTTGTTGCTGTCTTATCCACAATCTGGGTTTTTATTGGAGGGTGGTTTGATGGTGCCCAAAGTTCCAACCAATTAACATGGACGTGGACAGAGATGTGGGATTGGCTTGTTGCATTTCCATGGATACCTGCACTTTATACTGCGATGCGCGATGTTTCGGCAACAGAAACAGCCATAATTTATGGGCTGGAGCCACTGTGGGGAGCTGGATTTGCATGGTTTCTTCTTGGTGAAAGATGGGGAGCACTTGGATGGATCGGTGCAGCTCTTGTGCTTGGTGGAAGCCTTGTGGTGCAGATATTTGGGTCCTCAACTTCTAAAGAATTTATAGTTTCTGAAGAGAGCAAGGAGAAAGGTGATCTCCTAAGGGTTCCAGTTGACCAAAAATTACAAAATGGTCTCTCTACTTCTCCATTAGTTGTCAGGTCCAAAAAGGATCTAACAGATTTTCTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EamA

Protein Analysis

460

Amino Acids

50.85

Weight (kDa)

9.31

Isoelectric Point (pI)

44.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EamA PF00892 135 - 259 6e-09 EamA-like transporter family
EamA PF00892 270 - 407 1.2e-08 EamA-like transporter family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014745)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g17290 FvH4_5g17290 FvH4_5g17290
malus_domestica MD06G1226600.v1.1
prunus_persica Prupe.5G232800_v2.0.a1 Prupe.5G232900_v2.0.a1
pyrus_communis pycom06g20290
rosa_chinensis RchiOBHm_Chr7g0178481
rosa_laevigata RLG00000005443
rosa_multiflora Rmu_sc0010235.1_g000003
rosa_roxburghii Rroxscaffold_3G00274860
rosa_rugosa Rorug06G0417500
rosa_samantha Rh7AG016000 Rh7BG015600 Rh7CG016300 Rh7DG016000
rosa_wichuraiana Rw7G001260

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 736, 1063
Acc65I GGTACC 1 cut(s) 691
AccB1I GGYRCC 2 cut(s) 691, 980
AccB7I CCANNNNNTGG 2 cut(s) 806, 951
AccII CGCG 1 cut(s) 1077
AciI CCGC 3 cut(s) 127, 500, 505
AclWI GGATC 7 cut(s) 146, 608, 724, 729, 799, 1190, 1371
AcoI YGGCCR 1 cut(s) 652
AcsI RAATTY 4 cut(s) 57, 225, 846, 1250
AcuI CTGAAG 1 cut(s) 1284
AcyI GRCGYC 1 cut(s) 130
AdeI CACNNNGTG 1 cut(s) 557
AfaI GTAC 2 cut(s) 190, 693
AfiI CCNNNNNNNGG 6 cut(s) 123, 243, 622, 806, 951, 1127
AgsI TTSAA 7 cut(s) 63, 224, 230, 337, 470, 820, 876
AjiI CACGTC 1 cut(s) 1011
AjnI CCWGG 1 cut(s) 737
AluBI AGCT 4 cut(s) 66, 320, 1133, 1193
AluI AGCT 4 cut(s) 66, 320, 1133, 1193
Alw21I GWGCWC 2 cut(s) 276, 1174
Alw26I GTCTC 1 cut(s) 1331
AlwI GGATC 7 cut(s) 146, 608, 724, 729, 799, 1190, 1371
AoxI GGCC 1 cut(s) 652
ApeKI GCWGC 2 cut(s) 488, 1190
ApoI RAATTY 4 cut(s) 57, 225, 846, 1250
Asp718I GGTACC 1 cut(s) 691
AspLEI GCGC 1 cut(s) 1077
AspS9I GGNCC 3 cut(s) 481, 1233, 1353
AsuC2I CCSGG 1 cut(s) 555
AsuHPI GGTGA 6 cut(s) 43, 543, 569, 786, 1169, 1295
AsuII TTCGAA 1 cut(s) 859
AvaII GGWCC 3 cut(s) 481, 1233, 1353
AxyI CCTNAGG 1 cut(s) 1292
BaeGI GKGCMC 1 cut(s) 985
BanI GGYRCC 2 cut(s) 691, 980
Bbv12I GWGCWC 2 cut(s) 276, 1174
BbvI GCAGC 2 cut(s) 500, 1202
BccI CCATC 6 cut(s) 164, 236, 698, 971, 1158, 1173
BceAI ACGGC 2 cut(s) 140, 639
BciT130I CCWGG 1 cut(s) 739
BciVI GTATCC 1 cut(s) 1044
BcnI CCSGG 1 cut(s) 555
BcoDI GTCTC 1 cut(s) 1331
BfaI CTAG 3 cut(s) 608, 732, 902
BfmI CTRYAG 1 cut(s) 1379
BfuAI ACCTGC 2 cut(s) 736, 1063
BfuI GTATCC 1 cut(s) 1044
BglII AGATCT 1 cut(s) 811
BisI GCNGC 3 cut(s) 128, 489, 1191
BlsI GCNGC 3 cut(s) 129, 490, 1192
Bme1390I CCNGG 2 cut(s) 555, 739
Bme18I GGWCC 3 cut(s) 481, 1233, 1353
BmgBI CACGTC 1 cut(s) 1011
BmgT120I GGNCC 3 cut(s) 481, 1233, 1353
BmiI GGNNCC 7 cut(s) 346, 483, 693, 982, 1119, 1234, 1299
BmrFI CCNGG 2 cut(s) 555, 739
BmsI GCATC 4 cut(s) 395, 637, 668, 1062
BpmI CTGGAG 1 cut(s) 1136
Bpu14I TTCGAA 1 cut(s) 859
BpuEI CTTGAG 2 cut(s) 641, 847
BpuMI CCSGG 1 cut(s) 555
BsaHI GRCGYC 1 cut(s) 130
BsaI GGTCTC 1 cut(s) 1331
BsaJI CCNNGG 1 cut(s) 1046
BsaXI ACNNNNNCTCC 4 cut(s) 68, 98, 1109, 1139
Bsc4I CCNNNNNNNGG 6 cut(s) 123, 243, 622, 806, 951, 1127
Bse1I ACTGG 2 cut(s) 452, 1301
Bse21I CCTNAGG 1 cut(s) 1292
BseBI CCWGG 1 cut(s) 739
BseDI CCNNGG 1 cut(s) 1046
BseGI GGATG 3 cut(s) 319, 386, 1184
BseLI CCNNNNNNNGG 6 cut(s) 123, 243, 622, 806, 951, 1127
BseMII CTCAG 3 cut(s) 194, 510, 855
BseNI ACTGG 2 cut(s) 452, 1301
BseRI GAGGAG 1 cut(s) 415
BseSI GKGCMC 1 cut(s) 985
BseXI GCAGC 2 cut(s) 500, 1202
BseYI CCCAGC 1 cut(s) 484
BsgI GTGCAG 3 cut(s) 1041, 1209, 1241
Bsh1236I CGCG 1 cut(s) 1077
BshFI GGCC 1 cut(s) 654
BshNI GGYRCC 2 cut(s) 691, 980
BsiHKAI GWGCWC 2 cut(s) 276, 1174
BsiSI CCGG 1 cut(s) 554
BslFI GGGAC 1 cut(s) 259
BslI CCNNNNNNNGG 6 cut(s) 123, 243, 622, 806, 951, 1127
BsmAI GTCTC 1 cut(s) 1331
BsmFI GGGAC 1 cut(s) 259
BsnI GGCC 1 cut(s) 654
Bso31I GGTCTC 1 cut(s) 1331
Bsp119I TTCGAA 1 cut(s) 859
Bsp1286I GDGCHC 3 cut(s) 276, 985, 1174
Bsp19I CCATGG 1 cut(s) 1046
BspACI CCGC 3 cut(s) 127, 500, 505
BspANI GGCC 1 cut(s) 654
BspCNI CTCAG 3 cut(s) 193, 509, 856
BspFNI CGCG 1 cut(s) 1077
BspLI GGNNCC 7 cut(s) 346, 483, 693, 982, 1119, 1234, 1299
BspMI ACCTGC 2 cut(s) 736, 1063
BspPI GGATC 7 cut(s) 146, 608, 724, 729, 799, 1190, 1371
BspT104I TTCGAA 1 cut(s) 859
BspT107I GGYRCC 2 cut(s) 691, 980
BspTNI GGTCTC 1 cut(s) 1331
BsrI ACTGG 2 cut(s) 452, 1301
BssECI CCNNGG 1 cut(s) 1046
BssNI GRCGYC 1 cut(s) 130
BssT1I CCWWGG 1 cut(s) 1046
Bst2UI CCWGG 1 cut(s) 739
Bst4CI ACNGT 3 cut(s) 266, 682, 1125
Bst6I CTCTTC 4 cut(s) 101, 680, 821, 1260
BstACI GRCGYC 1 cut(s) 130
BstBI TTCGAA 1 cut(s) 859
BstC8I GCNNGC 1 cut(s) 652
BstDEI CTNAG 5 cut(s) 180, 321, 496, 864, 1292
BstDSI CCRYGG 1 cut(s) 1046
BstEII GGTNACC 1 cut(s) 31
BstENI CCTNNNNNAGG 1 cut(s) 620
BstF5I GGATG 3 cut(s) 319, 386, 1184
BstFNI CGCG 1 cut(s) 1077
BstHHI GCGC 1 cut(s) 1077
BstMAI GTCTC 1 cut(s) 1331
BstMWI GCNNNNNNNGC 5 cut(s) 72, 383, 497, 656, 1139
BstNI CCWGG 1 cut(s) 739
BstNSI RCATGY 1 cut(s) 856
BstPI GGTNACC 1 cut(s) 31
BstSCI CCNGG 2 cut(s) 553, 737
BstSFI CTRYAG 1 cut(s) 1379
BstSLI GKGCMC 1 cut(s) 985
BstUI CGCG 1 cut(s) 1077
BstV1I GCAGC 2 cut(s) 500, 1202
BstX2I RGATCY 4 cut(s) 151, 734, 811, 1363
BstYI RGATCY 4 cut(s) 151, 734, 811, 1363
Bsu36I CCTNAGG 1 cut(s) 1292
BsuI GTATCC 1 cut(s) 1044
BsuRI GGCC 1 cut(s) 654
BtgI CCRYGG 1 cut(s) 1046
BtgZI GCGATG 2 cut(s) 1085, 1092
BtrI CACGTC 1 cut(s) 1011
BtsCI GGATG 3 cut(s) 319, 386, 1184
BtsIMutI CAGTG 3 cut(s) 271, 931, 1121
BveI ACCTGC 2 cut(s) 736, 1063
Cac8I GCNNGC 1 cut(s) 652
CfoI GCGC 1 cut(s) 1077
Cfr13I GGNCC 3 cut(s) 481, 1233, 1353
CseI GACGC 1 cut(s) 119
Csp6I GTAC 2 cut(s) 189, 692
CviAII CATG 8 cut(s) 88, 478, 514, 754, 853, 1005, 1047, 1143
CviQI GTAC 2 cut(s) 189, 692
DdeI CTNAG 5 cut(s) 180, 321, 496, 864, 1292
DraIII CACNNNGTG 1 cut(s) 557
EaeI YGGCCR 1 cut(s) 652
Eam1104I CTCTTC 4 cut(s) 101, 680, 821, 1260
EarI CTCTTC 4 cut(s) 101, 680, 821, 1260
Eco130I CCWWGG 1 cut(s) 1046
Eco31I GGTCTC 1 cut(s) 1331
Eco47I GGWCC 3 cut(s) 481, 1233, 1353
Eco57I CTGAAG 1 cut(s) 1284
Eco81I CCTNAGG 1 cut(s) 1292
Eco91I GGTNACC 1 cut(s) 31
EcoNI CCTNNNNNAGG 1 cut(s) 620
EcoO109I RGGNCCY 1 cut(s) 1233
EcoO65I GGTNACC 1 cut(s) 31
EcoRI GAATTC 1 cut(s) 846
EcoRII CCWGG 1 cut(s) 737
EcoT14I CCWWGG 1 cut(s) 1046
ErhI CCWWGG 1 cut(s) 1046
FaeI CATG 8 cut(s) 91, 481, 517, 757, 856, 1008, 1050, 1146
FalI AAGNNNNNCTT 4 cut(s) 365, 397, 881, 913
FaqI GGGAC 1 cut(s) 259
FatI CATG 8 cut(s) 87, 477, 513, 753, 852, 1004, 1046, 1142
FauNDI CATATG 1 cut(s) 283
Fnu4HI GCNGC 3 cut(s) 128, 489, 1191
FokI GGATG 3 cut(s) 326, 373, 1191
Fsp4HI GCNGC 3 cut(s) 128, 489, 1191
FspBI CTAG 3 cut(s) 608, 732, 902
GlaI GCGC 1 cut(s) 1076
GluI GCNGC 3 cut(s) 128, 489, 1191
GsaI CCCAGC 1 cut(s) 488
GsuI CTGGAG 1 cut(s) 1136
HaeIII GGCC 1 cut(s) 654
HapII CCGG 1 cut(s) 554
HgaI GACGC 1 cut(s) 119
HhaI GCGC 1 cut(s) 1077
Hin1I GRCGYC 1 cut(s) 130
Hin1II CATG 8 cut(s) 91, 481, 517, 757, 856, 1008, 1050, 1146
Hin6I GCGC 1 cut(s) 1075
HinP1I GCGC 1 cut(s) 1075
HincII GTYRAC 1 cut(s) 1306
HindII GTYRAC 1 cut(s) 1306
HinfI GANTC 1 cut(s) 44
HpaII CCGG 1 cut(s) 554
HphI GGTGA 6 cut(s) 43, 543, 569, 786, 1169, 1295
Hpy166II GTNNAC 3 cut(s) 635, 1014, 1306
Hpy188I TCNGA 6 cut(s) 183, 567, 646, 746, 845, 1264
Hpy188III TCNNGA 5 cut(s) 298, 598, 620, 826, 876
Hpy8I GTNNAC 3 cut(s) 635, 1014, 1306
Hpy99I CGWCG 1 cut(s) 360
HpyAV CCTTC 4 cut(s) 304, 502, 626, 883
HpyCH4III ACNGT 3 cut(s) 266, 682, 1125
HpyCH4IV ACGT 1 cut(s) 1010
HpyF10VI GCNNNNNNNGC 5 cut(s) 72, 383, 497, 656, 1139
HpyF3I CTNAG 5 cut(s) 180, 321, 496, 864, 1292
HpySE526I ACGT 1 cut(s) 1010
Hsp92I GRCGYC 1 cut(s) 130
Hsp92II CATG 8 cut(s) 91, 481, 517, 757, 856, 1008, 1050, 1146
HspAI GCGC 1 cut(s) 1075
KpnI GGTACC 1 cut(s) 695
LmnI GCTCC 3 cut(s) 1117, 1130, 1169
Lsp1109I GCAGC 2 cut(s) 500, 1202
LweI GCATC 4 cut(s) 395, 637, 668, 1062
MaeI CTAG 3 cut(s) 608, 732, 902
MaeII ACGT 1 cut(s) 1010
MaeIII GTNAC 2 cut(s) 31, 443
MboII GAAGA 8 cut(s) 88, 301, 304, 406, 667, 808, 1142, 1277
MfeI CAATTG 1 cut(s) 429
MflI RGATCY 4 cut(s) 151, 734, 811, 1363
MhlI GDGCHC 3 cut(s) 276, 985, 1174
MlyI GAGTC 1 cut(s) 38
MmeI TCCRAC 2 cut(s) 787, 1017
MseI TTAA 2 cut(s) 459, 1001
MslI CAYNNNNRTG 4 cut(s) 267, 1009, 1045, 1177
MspA1I CMGCKG 1 cut(s) 500
MspI CCGG 1 cut(s) 554
MspR9I CCNGG 2 cut(s) 555, 739
MunI CAATTG 1 cut(s) 429
MvaI CCWGG 1 cut(s) 739
MvnI CGCG 1 cut(s) 1077
MwoI GCNNNNNNNGC 5 cut(s) 72, 383, 497, 656, 1139
NciI CCSGG 1 cut(s) 555
NcoI CCATGG 1 cut(s) 1046
NdeI CATATG 1 cut(s) 283
NlaIII CATG 8 cut(s) 91, 481, 517, 757, 856, 1008, 1050, 1146
NlaIV GGNNCC 7 cut(s) 346, 483, 693, 982, 1119, 1234, 1299
NmuCI GTSAC 2 cut(s) 31, 443
NspI RCATGY 1 cut(s) 856
NspV TTCGAA 1 cut(s) 859
PflMI CCANNNNNTGG 2 cut(s) 806, 951
PkrI GCNGC 3 cut(s) 129, 490, 1192
PleI GAGTC 1 cut(s) 38
PpsI GAGTC 1 cut(s) 38
PpuMI RGGWCCY 1 cut(s) 1233
Psp5II RGGWCCY 1 cut(s) 1233
Psp6I CCWGG 1 cut(s) 737
PspEI GGTNACC 1 cut(s) 31
PspFI CCCAGC 1 cut(s) 484
PspGI CCWGG 1 cut(s) 737
PspN4I GGNNCC 7 cut(s) 346, 483, 693, 982, 1119, 1234, 1299
PspPI GGNCC 3 cut(s) 481, 1233, 1353
PspPPI RGGWCCY 1 cut(s) 1233
PsuI RGATCY 4 cut(s) 151, 734, 811, 1363
RsaI GTAC 2 cut(s) 190, 693
RsaNI GTAC 2 cut(s) 189, 692
RseI CAYNNNNRTG 4 cut(s) 267, 1009, 1045, 1177
SaqAI TTAA 2 cut(s) 459, 1001
SatI GCNGC 3 cut(s) 128, 489, 1191
Sau96I GGNCC 3 cut(s) 481, 1233, 1353
SchI GAGTC 1 cut(s) 38
ScrFI CCNGG 2 cut(s) 555, 739
SduI GDGCHC 3 cut(s) 276, 985, 1174
SfaNI GCATC 4 cut(s) 395, 637, 668, 1062
SfcI CTRYAG 1 cut(s) 1379
SfuI TTCGAA 1 cut(s) 859
SinI GGWCC 3 cut(s) 481, 1233, 1353
SmiMI CAYNNNNRTG 4 cut(s) 267, 1009, 1045, 1177
SmlI CTYRAG 2 cut(s) 620, 826
SmoI CTYRAG 2 cut(s) 620, 826
SsiI CCGC 3 cut(s) 127, 500, 505
SspI AATATT 1 cut(s) 837
SspMI CTAG 3 cut(s) 608, 732, 902
StyD4I CCNGG 2 cut(s) 553, 737
StyI CCWWGG 1 cut(s) 1046
TaaI ACNGT 3 cut(s) 266, 682, 1125
TaiI ACGT 1 cut(s) 1013
TaqI TCGA 2 cut(s) 547, 859
TauI GCSGC 1 cut(s) 130
Tru1I TTAA 2 cut(s) 459, 1001
Tru9I TTAA 2 cut(s) 459, 1001
TscAI CASTG 3 cut(s) 271, 931, 1128
TseFI GTSAC 2 cut(s) 31, 443
TseI GCWGC 2 cut(s) 488, 1190
Tsp45I GTSAC 2 cut(s) 31, 443
TspDTI ATGAA 3 cut(s) 135, 156, 330
TspRI CASTG 3 cut(s) 271, 931, 1128
Van91I CCANNNNNTGG 2 cut(s) 806, 951
VpaK11BI GGWCC 3 cut(s) 481, 1233, 1353
XagI CCTNNNNNAGG 1 cut(s) 620
XapI RAATTY 4 cut(s) 57, 225, 846, 1250
XceI RCATGY 1 cut(s) 856
XspI CTAG 3 cut(s) 608, 732, 902
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.