MD07G1011200.v1.1

Folate-sensitive fragile site protein Fra10Ac1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0014301
Physical Location & Seq
Forward (+)
1014590 .. 1019833
5244 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1011200.v1.1.491

Sequence Viewer

Length: 744 bp
ATGGCGTCGTTTGGGTCTTTAAAATCTGCAATCTTCGACAGAGAAGAGAGAAAACAGCAGTACCAGGCTCACATTCTCGGCCTCAACGCTTACGACCGCCACAAAAAGTTCGTCAAAGATTACGTTAACTTTTATGGTAGACAAGAATCTTCACATGTCAAGCTCCCAGTTAAAACAGATCAAGACACCCTAAGAGAAGGGTACCGGTTCATAAGATCTGAGGAAGATGATATGGACCGATCTTGGGAGCAAAGGCTGGTAAAGCGATACTATGACAAGCTGTTTAAAGAATACTGCATAGCAGACATGTCACATTACAAGAGTGGTAAGATTGGTCTAAGGTGGAGGACAGAGAAGGAAGTCGTATCTGGTAAAGGGCAGTTCATATGTGGTAACAAACATTGTGATGTAAAGGATGGTTTAGCAAGCTATGAGGTGAACTTTTCTTATTTTGAAGCTGGAGAAAACAAACAAGCCCTTGTCAAATTGGTAACTTGTCAGAGATGCTCTGAAAAGCTTCATTACAAAAGATTGAAAGAGAAAGAGCAAATGGAAAAAGAAAAGAAGGAAGAGAACAGAAGAAAGAGGAATCGATCAGGTGACGGTGATGATACTGATAGTGAGGGGAGCAAAGAAAGGAGAAAAGGGAAAAAGGCTTCAATCTCTACTAGAGAAGATAGAGTTGATGATGAAGACGATGATACAGATAAGTTTGAAGAGTATCTCGAGGGAATGTTTCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Fra10Ac1

Protein Analysis

248

Amino Acids

29.34

Weight (kDa)

8.39

Isoelectric Point (pI)

51.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Fra10Ac1 PF09725 59 - 175 4.6e-51 Folate-sensitive fragile site protein Fra10Ac1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014301)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G15030 AT4G15030 AT4G15030
fragaria_vesca FvH4_7g01110
malus_domestica MD02G1310300.v1.1 MD07G1011200.v1.1
prunus_persica Prupe.2G014400_v2.0.a1
pyrus_communis pycom07g00930
rosa_chinensis RchiOBHm_Chr1g0316111
rosa_laevigata RLG00000030694
rosa_multiflora Rmu_sc0002531.1_g000028
rosa_roxburghii Rroxscaffold_4G00331130
rosa_rugosa Rorug01G0009100
rosa_samantha Rh1AG019100 Rh1BG014100 Rh1CG016700
rosa_wichuraiana Rw1G001240

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 201
AccB1I GGYRCC 1 cut(s) 201
AccI GTMKAC 1 cut(s) 139
AciI CCGC 1 cut(s) 97
AcyI GRCGYC 1 cut(s) 5
AfaI GTAC 2 cut(s) 62, 203
AfiI CCNNNNNNNGG 1 cut(s) 244
AflIII ACRYGT 2 cut(s) 154, 306
AgeI ACCGGT 1 cut(s) 204
AgsI TTSAA 4 cut(s) 455, 535, 660, 716
AjnI CCWGG 1 cut(s) 63
AluBI AGCT 5 cut(s) 163, 280, 429, 458, 517
AluI AGCT 5 cut(s) 163, 280, 429, 458, 517
Ama87I CYCGRG 1 cut(s) 725
AoxI GGCC 1 cut(s) 79
ArsI GACNNNNNNTTYG 2 cut(s) 96, 128
AsiGI ACCGGT 1 cut(s) 204
Asp700I GAANNNNTTC 1 cut(s) 516
Asp718I GGTACC 1 cut(s) 201
AspS9I GGNCC 1 cut(s) 235
AsuHPI GGTGA 3 cut(s) 448, 611, 617
AvaI CYCGRG 1 cut(s) 725
AvaII GGWCC 1 cut(s) 235
BaeI ACNNNNGTAYC 2 cut(s) 44, 77
BanI GGYRCC 1 cut(s) 201
BbsI GAAGAC 1 cut(s) 699
BccI CCATC 1 cut(s) 410
BciT130I CCWGG 1 cut(s) 65
BfaI CTAG 1 cut(s) 669
BglII AGATCT 1 cut(s) 215
Bme1390I CCNGG 1 cut(s) 65
Bme18I GGWCC 1 cut(s) 235
BmeT110I CYCGRG 1 cut(s) 725
BmgT120I GGNCC 1 cut(s) 235
BmiI GGNNCC 1 cut(s) 203
BmrFI CCNGG 1 cut(s) 65
BmrI ACTGGG 1 cut(s) 161
BmsI GCATC 1 cut(s) 494
BmuI ACTGGG 1 cut(s) 161
BpiI GAAGAC 1 cut(s) 699
BpmI CTGGAG 1 cut(s) 480
Bsa29I ATCGAT 1 cut(s) 592
BsaHI GRCGYC 1 cut(s) 5
BsaWI WCCGGW 1 cut(s) 204
Bsc4I CCNNNNNNNGG 1 cut(s) 244
Bse118I RCCGGY 1 cut(s) 204
Bse1I ACTGG 1 cut(s) 167
BseBI CCWGG 1 cut(s) 65
BseCI ATCGAT 1 cut(s) 592
BseGI GGATG 1 cut(s) 421
BseLI CCNNNNNNNGG 1 cut(s) 244
BseMII CTCAG 1 cut(s) 210
BseNI ACTGG 1 cut(s) 167
Bsh1285I CGRYCG 1 cut(s) 97
BshFI GGCC 1 cut(s) 81
BshNI GGYRCC 1 cut(s) 201
BshTI ACCGGT 1 cut(s) 204
BshVI ATCGAT 1 cut(s) 592
BsiEI CGRYCG 1 cut(s) 97
BsiHKCI CYCGRG 1 cut(s) 725
BsiSI CCGG 1 cut(s) 205
BslI CCNNNNNNNGG 1 cut(s) 244
BsnI GGCC 1 cut(s) 81
BsoBI CYCGRG 1 cut(s) 725
Bsp143I GATC 4 cut(s) 178, 215, 239, 593
BspACI CCGC 1 cut(s) 97
BspANI GGCC 1 cut(s) 81
BspCNI CTCAG 1 cut(s) 211
BspDI ATCGAT 1 cut(s) 592
BspLI GGNNCC 1 cut(s) 203
BspT107I GGYRCC 1 cut(s) 201
BsrFI RCCGGY 1 cut(s) 204
BsrI ACTGG 1 cut(s) 167
BssAI RCCGGY 1 cut(s) 204
BssMI GATC 4 cut(s) 178, 215, 239, 593
BssNI GRCGYC 1 cut(s) 5
Bst2UI CCWGG 1 cut(s) 65
Bst4CI ACNGT 1 cut(s) 605
Bst6I CTCTTC 3 cut(s) 39, 564, 711
BstACI GRCGYC 1 cut(s) 5
BstC8I GCNNGC 1 cut(s) 427
BstDEI CTNAG 3 cut(s) 191, 219, 338
BstF5I GGATG 1 cut(s) 421
BstKTI GATC 4 cut(s) 181, 218, 242, 596
BstMBI GATC 4 cut(s) 178, 215, 239, 593
BstMCI CGRYCG 1 cut(s) 97
BstMWI GCNNNNNNNGC 1 cut(s) 262
BstNI CCWGG 1 cut(s) 65
BstNSI RCATGY 2 cut(s) 158, 310
BstSCI CCNGG 1 cut(s) 63
BstV2I GAAGAC 1 cut(s) 699
BstX2I RGATCY 1 cut(s) 215
BstYI RGATCY 1 cut(s) 215
Bsu15I ATCGAT 1 cut(s) 592
BsuRI GGCC 1 cut(s) 81
BsuTUI ATCGAT 1 cut(s) 592
BtsCI GGATG 1 cut(s) 421
Cac8I GCNNGC 1 cut(s) 427
Cfr10I RCCGGY 1 cut(s) 204
Cfr13I GGNCC 1 cut(s) 235
ClaI ATCGAT 1 cut(s) 592
Csp6I GTAC 2 cut(s) 61, 202
CspAI ACCGGT 1 cut(s) 204
CviAII CATG 2 cut(s) 155, 307
CviQI GTAC 2 cut(s) 61, 202
DdeI CTNAG 3 cut(s) 191, 219, 338
DpnI GATC 4 cut(s) 180, 217, 241, 595
DpnII GATC 4 cut(s) 178, 215, 239, 593
DraI TTTAAA 2 cut(s) 21, 286
Eam1104I CTCTTC 3 cut(s) 39, 564, 711
EarI CTCTTC 3 cut(s) 39, 564, 711
Eco47I GGWCC 1 cut(s) 235
Eco88I CYCGRG 1 cut(s) 725
EcoRII CCWGG 1 cut(s) 63
FaeI CATG 2 cut(s) 158, 310
FatI CATG 2 cut(s) 154, 306
FauNDI CATATG 1 cut(s) 386
FblI GTMKAC 1 cut(s) 139
FokI GGATG 1 cut(s) 428
FspBI CTAG 1 cut(s) 669
GsuI CTGGAG 1 cut(s) 480
HaeIII GGCC 1 cut(s) 81
HapII CCGG 1 cut(s) 205
Hin1I GRCGYC 1 cut(s) 5
Hin1II CATG 2 cut(s) 158, 310
HincII GTYRAC 1 cut(s) 127
HindII GTYRAC 1 cut(s) 127
HindIII AAGCTT 1 cut(s) 515
HinfI GANTC 2 cut(s) 146, 589
HpaI GTTAAC 1 cut(s) 127
HpaII CCGG 1 cut(s) 205
HphI GGTGA 3 cut(s) 448, 611, 617
Hpy166II GTNNAC 3 cut(s) 127, 140, 439
Hpy188I TCNGA 3 cut(s) 220, 501, 511
Hpy188III TCNNGA 2 cut(s) 182, 725
Hpy8I GTNNAC 3 cut(s) 127, 140, 439
Hpy99I CGWCG 1 cut(s) 10
HpyAV CCTTC 3 cut(s) 191, 349, 559
HpyCH4III ACNGT 1 cut(s) 605
HpyCH4IV ACGT 1 cut(s) 123
HpyCH4V TGCA 2 cut(s) 29, 297
HpyF10VI GCNNNNNNNGC 1 cut(s) 262
HpyF3I CTNAG 3 cut(s) 191, 219, 338
HpySE526I ACGT 1 cut(s) 123
Hsp92I GRCGYC 1 cut(s) 5
Hsp92II CATG 2 cut(s) 158, 310
KpnI GGTACC 1 cut(s) 205
KspAI GTTAAC 1 cut(s) 127
Kzo9I GATC 4 cut(s) 178, 215, 239, 593
LmnI GCTCC 3 cut(s) 168, 247, 627
LpnPI CCDG 8 cut(s) 50, 77, 180, 218, 242, 354, 444, 582
LweI GCATC 1 cut(s) 494
MaeI CTAG 1 cut(s) 669
MaeII ACGT 1 cut(s) 123
MaeIII GTNAC 4 cut(s) 309, 392, 490, 599
MalI GATC 4 cut(s) 180, 217, 241, 595
MboI GATC 4 cut(s) 178, 215, 239, 593
MboII GAAGA 9 cut(s) 25, 56, 141, 236, 581, 591, 686, 704, 728
MflI RGATCY 1 cut(s) 215
MluCI AATT 1 cut(s) 485
MnlI CCTC 7 cut(s) 92, 214, 339, 427, 579, 616, 721
MroXI GAANNNNTTC 1 cut(s) 516
MseI TTAA 4 cut(s) 20, 126, 171, 285
MslI CAYNNNNRTG 1 cut(s) 405
MspI CCGG 1 cut(s) 205
MspR9I CCNGG 1 cut(s) 65
MvaI CCWGG 1 cut(s) 65
MwoI GCNNNNNNNGC 1 cut(s) 262
NdeI CATATG 1 cut(s) 386
NdeII GATC 4 cut(s) 178, 215, 239, 593
NlaIII CATG 2 cut(s) 158, 310
NlaIV GGNNCC 1 cut(s) 203
NmeAIII GCCGAG 1 cut(s) 57
NmuCI GTSAC 2 cut(s) 309, 599
NspI RCATGY 2 cut(s) 158, 310
PaeR7I CTCGAG 1 cut(s) 725
PciI ACATGT 2 cut(s) 154, 306
PdmI GAANNNNTTC 1 cut(s) 516
PfeI GAWTC 2 cut(s) 146, 589
PinAI ACCGGT 1 cut(s) 204
PscI ACATGT 2 cut(s) 154, 306
Psp6I CCWGG 1 cut(s) 63
PspGI CCWGG 1 cut(s) 63
PspN4I GGNNCC 1 cut(s) 203
PspPI GGNCC 1 cut(s) 235
PsuI RGATCY 1 cut(s) 215
RsaI GTAC 2 cut(s) 62, 203
RsaNI GTAC 2 cut(s) 61, 202
RseI CAYNNNNRTG 1 cut(s) 405
SaqAI TTAA 4 cut(s) 20, 126, 171, 285
Sau3AI GATC 4 cut(s) 178, 215, 239, 593
Sau96I GGNCC 1 cut(s) 235
ScrFI CCNGG 1 cut(s) 65
SetI ASST 9 cut(s) 126, 165, 282, 344, 431, 438, 460, 519, 601
SfaNI GCATC 1 cut(s) 494
Sfr274I CTCGAG 1 cut(s) 725
SinI GGWCC 1 cut(s) 235
SlaI CTCGAG 1 cut(s) 725
SmiMI CAYNNNNRTG 1 cut(s) 405
SmlI CTYRAG 1 cut(s) 725
SmoI CTYRAG 1 cut(s) 725
Sse9I AATT 1 cut(s) 485
SsiI CCGC 1 cut(s) 97
SspMI CTAG 1 cut(s) 669
StyD4I CCNGG 1 cut(s) 63
TaaI ACNGT 1 cut(s) 605
TaiI ACGT 1 cut(s) 126
TaqI TCGA 3 cut(s) 36, 592, 726
TaqII GACCGA 1 cut(s) 252
TasI AATT 1 cut(s) 485
TfiI GAWTC 2 cut(s) 146, 589
Tru1I TTAA 4 cut(s) 20, 126, 171, 285
Tru9I TTAA 4 cut(s) 20, 126, 171, 285
TseFI GTSAC 2 cut(s) 309, 599
Tsp45I GTSAC 2 cut(s) 309, 599
TspDTI ATGAA 4 cut(s) 199, 373, 509, 705
VpaK11BI GGWCC 1 cut(s) 235
XceI RCATGY 2 cut(s) 158, 310
XhoI CTCGAG 1 cut(s) 725
XmiI GTMKAC 1 cut(s) 139
XmnI GAANNNNTTC 1 cut(s) 516
XspI CTAG 1 cut(s) 669
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.