Rroxscaffold_4G00331130

Folate-sensitive fragile site protein Fra10Ac1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0014301
Physical Location & Seq
Reverse (-)
64930110 .. 64940478
10369 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00331130.1

Sequence Viewer

Length: 756 bp
ATGGCCTCGTTTGCGTCGTTAAAATCTGCAATCTTCGACAGAGAAGAGCGAAAACAGCAGTACCAGGATCACATTCGCGGCCTCAACGCTTACGAACGCCACAAGAAGTTTGTCAAAGATTACGTTAGCTATTATGGTAGAGAAGGATCTTCACACGTGAAGTTGCCTGTCAAAACTGATCAAGACACCCTAAGAGAAGGGTACCGGTTTATAAGATCCGAGGAAGATGACATGGACACATCTTGGGAGCAGAGGCTGGTGAAGCGATACTATGACAAGCTGTTTAAAGAATATTGCATAGCTGATATGTCACATTACAAGAGTGGTAAGATTGGTCTTAGGTGGAGGACAGAAAAGGAAGTCGTATCTGGTAAAGGGCAGTTCATATGTGGTAGCAAACATTGCGATCTAAAAGATGGCTTAGCCAGCTACGAGGCAAGTGTTTACTATTTGTCTACAGTAGAACAGCTACATATTCTCCCAAAGAGATCGAAATGTCAAAGAAAAATTATAAAACAAACATATAAAGAAAGATTTGAGAGGGAGCGAGCGAGAAAGAATGAGTCCAACCTCCGCGACTTCGAATCCCTAAAAACCACCGCAGCTCTTCCGGAGTTTCTATCCCTTACTTCGCACACCACTAAACCTTCTTCTTTCTCTCCCTCCTTGGTTACTCAGAAAAAGTGGAAAAACTGCAAAATTGAAAACATAGGAGAAGAAATTTTCGAACACGTTTTGGAGGGATTATTCCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Fra10Ac1

Protein Analysis

251

Amino Acids

29.65

Weight (kDa)

9.21

Isoelectric Point (pI)

47.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Fra10Ac1 PF09725 59 - 170 7.2e-39 Folate-sensitive fragile site protein Fra10Ac1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014301)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G15030 AT4G15030 AT4G15030
fragaria_vesca FvH4_7g01110
malus_domestica MD02G1310300.v1.1 MD07G1011200.v1.1
prunus_persica Prupe.2G014400_v2.0.a1
pyrus_communis pycom07g00930
rosa_chinensis RchiOBHm_Chr1g0316111
rosa_laevigata RLG00000030694
rosa_multiflora Rmu_sc0002531.1_g000028
rosa_roxburghii Rroxscaffold_4G00331130
rosa_rugosa Rorug01G0009100
rosa_samantha Rh1AG019100 Rh1BG014100 Rh1CG016700
rosa_wichuraiana Rw1G001240

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 212, 512
Acc65I GGTACC 1 cut(s) 201
AccB1I GGYRCC 1 cut(s) 201
AccI GTMKAC 1 cut(s) 455
AccII CGCG 2 cut(s) 78, 576
AccIII TCCGGA 1 cut(s) 610
AciI CCGC 3 cut(s) 78, 574, 600
AclWI GGATC 3 cut(s) 75, 154, 210
AcsI RAATTY 1 cut(s) 720
AcvI CACGTG 1 cut(s) 157
AfaI GTAC 2 cut(s) 62, 203
AflIII ACRYGT 2 cut(s) 154, 730
AgeI ACCGGT 1 cut(s) 204
AgsI TTSAA 1 cut(s) 704
AjnI CCWGG 1 cut(s) 63
AluBI AGCT 6 cut(s) 129, 280, 302, 429, 469, 605
AluI AGCT 6 cut(s) 129, 280, 302, 429, 469, 605
AlwI GGATC 3 cut(s) 75, 154, 210
AlwNI CAGNNNCTG 1 cut(s) 256
Aor13HI TCCGGA 1 cut(s) 610
AoxI GGCC 2 cut(s) 3, 79
ApeKI GCWGC 1 cut(s) 602
ApoI RAATTY 1 cut(s) 720
AsiGI ACCGGT 1 cut(s) 204
Asp718I GGTACC 1 cut(s) 201
AsuHPI GGTGA 1 cut(s) 271
AsuII TTCGAA 2 cut(s) 582, 726
BaeI ACNNNNGTAYC 2 cut(s) 44, 77
BanI GGYRCC 1 cut(s) 201
BbrPI CACGTG 1 cut(s) 157
BbvI GCAGC 1 cut(s) 614
BccI CCATC 1 cut(s) 410
BciT130I CCWGG 1 cut(s) 65
BclI TGATCA 1 cut(s) 178
BfmI CTRYAG 1 cut(s) 456
BisI GCNGC 2 cut(s) 79, 603
BlpI GCTNAGC 1 cut(s) 421
BlsI GCNGC 2 cut(s) 80, 604
Bme1390I CCNGG 1 cut(s) 65
BmiI GGNNCC 1 cut(s) 203
BmrFI CCNGG 1 cut(s) 65
Bpu1102I GCTNAGC 1 cut(s) 421
Bpu14I TTCGAA 2 cut(s) 582, 726
BsaAI YACGTR 1 cut(s) 157
BsaJI CCNNGG 2 cut(s) 219, 666
BsaWI WCCGGW 2 cut(s) 204, 610
BsaXI ACNNNNNCTCC 2 cut(s) 462, 492
Bse118I RCCGGY 1 cut(s) 204
Bse3DI GCAATG 1 cut(s) 400
BseAI TCCGGA 1 cut(s) 610
BseBI CCWGG 1 cut(s) 65
BseDI CCNNGG 2 cut(s) 219, 666
BseMI GCAATG 1 cut(s) 400
BseMII CTCAG 1 cut(s) 689
BseXI GCAGC 1 cut(s) 614
Bsh1236I CGCG 2 cut(s) 78, 576
BshFI GGCC 2 cut(s) 5, 81
BshNI GGYRCC 1 cut(s) 201
BshTI ACCGGT 1 cut(s) 204
BsiSI CCGG 2 cut(s) 205, 611
BsnI GGCC 2 cut(s) 5, 81
Bsp119I TTCGAA 2 cut(s) 582, 726
Bsp13I TCCGGA 1 cut(s) 610
Bsp143I GATC 6 cut(s) 67, 146, 178, 215, 406, 488
Bsp1720I GCTNAGC 1 cut(s) 421
BspACI CCGC 3 cut(s) 78, 574, 600
BspANI GGCC 2 cut(s) 5, 81
BspCNI CTCAG 1 cut(s) 688
BspEI TCCGGA 1 cut(s) 610
BspFNI CGCG 2 cut(s) 78, 576
BspLI GGNNCC 1 cut(s) 203
BspPI GGATC 3 cut(s) 75, 154, 210
BspQI GCTCTTC 2 cut(s) 39, 612
BspT104I TTCGAA 2 cut(s) 582, 726
BspT107I GGYRCC 1 cut(s) 201
BsrDI GCAATG 1 cut(s) 400
BsrFI RCCGGY 1 cut(s) 204
BssAI RCCGGY 1 cut(s) 204
BssECI CCNNGG 2 cut(s) 219, 666
BssMI GATC 6 cut(s) 67, 146, 178, 215, 406, 488
BssT1I CCWWGG 1 cut(s) 666
Bst2UI CCWGG 1 cut(s) 65
Bst4CI ACNGT 1 cut(s) 460
Bst6I CTCTTC 2 cut(s) 39, 612
BstAPI GCANNNNNTGC 1 cut(s) 402
BstBAI YACGTR 1 cut(s) 157
BstBI TTCGAA 2 cut(s) 582, 726
BstC8I GCNNGC 2 cut(s) 427, 549
BstDEI CTNAG 4 cut(s) 191, 338, 421, 675
BstFNI CGCG 2 cut(s) 78, 576
BstKTI GATC 6 cut(s) 70, 149, 181, 218, 409, 491
BstMBI GATC 6 cut(s) 67, 146, 178, 215, 406, 488
BstMWI GCNNNNNNNGC 5 cut(s) 11, 55, 262, 402, 426
BstNI CCWGG 1 cut(s) 65
BstSCI CCNGG 1 cut(s) 63
BstSFI CTRYAG 1 cut(s) 456
BstUI CGCG 2 cut(s) 78, 576
BstV1I GCAGC 1 cut(s) 614
BstX2I RGATCY 2 cut(s) 146, 215
BstYI RGATCY 2 cut(s) 146, 215
BsuRI GGCC 2 cut(s) 5, 81
Cac8I GCNNGC 2 cut(s) 427, 549
CaiI CAGNNNCTG 1 cut(s) 256
Cfr10I RCCGGY 1 cut(s) 204
CseI GACGC 1 cut(s) 3
Csp6I GTAC 2 cut(s) 61, 202
CspAI ACCGGT 1 cut(s) 204
CviAII CATG 1 cut(s) 232
CviQI GTAC 2 cut(s) 61, 202
DdeI CTNAG 4 cut(s) 191, 338, 421, 675
DpnI GATC 6 cut(s) 69, 148, 180, 217, 408, 490
DpnII GATC 6 cut(s) 67, 146, 178, 215, 406, 488
DraI TTTAAA 1 cut(s) 286
Eam1104I CTCTTC 2 cut(s) 39, 612
EarI CTCTTC 2 cut(s) 39, 612
Eco130I CCWWGG 1 cut(s) 666
Eco72I CACGTG 1 cut(s) 157
EcoRII CCWGG 1 cut(s) 63
EcoT14I CCWWGG 1 cut(s) 666
ErhI CCWWGG 1 cut(s) 666
FaeI CATG 1 cut(s) 235
FatI CATG 1 cut(s) 231
FauNDI CATATG 1 cut(s) 386
FbaI TGATCA 1 cut(s) 178
FblI GTMKAC 1 cut(s) 455
Fnu4HI GCNGC 2 cut(s) 79, 603
Fsp4HI GCNGC 2 cut(s) 79, 603
GluI GCNGC 2 cut(s) 79, 603
HaeIII GGCC 2 cut(s) 5, 81
HapII CCGG 2 cut(s) 205, 611
HgaI GACGC 1 cut(s) 3
Hin1II CATG 1 cut(s) 235
HinfI GANTC 2 cut(s) 563, 584
HpaII CCGG 2 cut(s) 205, 611
HphI GGTGA 1 cut(s) 271
Hpy166II GTNNAC 2 cut(s) 445, 456
Hpy188I TCNGA 2 cut(s) 220, 678
Hpy188III TCNNGA 2 cut(s) 182, 611
Hpy8I GTNNAC 2 cut(s) 445, 456
Hpy99I CGWCG 1 cut(s) 19
HpyAV CCTTC 3 cut(s) 137, 191, 657
HpyCH4III ACNGT 1 cut(s) 460
HpyCH4IV ACGT 3 cut(s) 123, 156, 732
HpyCH4V TGCA 3 cut(s) 29, 297, 696
HpyF10VI GCNNNNNNNGC 5 cut(s) 11, 55, 262, 402, 426
HpyF3I CTNAG 4 cut(s) 191, 338, 421, 675
HpySE526I ACGT 3 cut(s) 123, 156, 732
Hsp92II CATG 1 cut(s) 235
Kpn2I TCCGGA 1 cut(s) 610
KpnI GGTACC 1 cut(s) 205
Ksp22I TGATCA 1 cut(s) 178
Kzo9I GATC 6 cut(s) 67, 146, 178, 215, 406, 488
LguI GCTCTTC 2 cut(s) 39, 612
LmnI GCTCC 2 cut(s) 247, 544
LpnPI CCDG 8 cut(s) 50, 77, 180, 218, 242, 354, 439, 624
Lsp1109I GCAGC 1 cut(s) 614
MaeII ACGT 3 cut(s) 123, 156, 732
MaeIII GTNAC 2 cut(s) 309, 670
MalI GATC 6 cut(s) 69, 148, 180, 217, 408, 490
MboI GATC 6 cut(s) 67, 146, 178, 215, 406, 488
MboII GAAGA 7 cut(s) 25, 56, 141, 236, 599, 642, 728
MflI RGATCY 2 cut(s) 146, 215
MluCI AATT 3 cut(s) 507, 699, 720
MlyI GAGTC 1 cut(s) 572
MmeI TCCRAC 1 cut(s) 591
MroI TCCGGA 1 cut(s) 610
MseI TTAA 2 cut(s) 20, 285
MspI CCGG 2 cut(s) 205, 611
MspR9I CCNGG 1 cut(s) 65
MvaI CCWGG 1 cut(s) 65
MvnI CGCG 2 cut(s) 78, 576
MwoI GCNNNNNNNGC 5 cut(s) 11, 55, 262, 402, 426
NdeI CATATG 1 cut(s) 386
NdeII GATC 6 cut(s) 67, 146, 178, 215, 406, 488
NlaIII CATG 1 cut(s) 235
NlaIV GGNNCC 1 cut(s) 203
NmuCI GTSAC 1 cut(s) 309
NspV TTCGAA 2 cut(s) 582, 726
PciSI GCTCTTC 2 cut(s) 39, 612
PcsI WCGNNNNNNNCGW 1 cut(s) 14
PfeI GAWTC 1 cut(s) 584
PinAI ACCGGT 1 cut(s) 204
PkrI GCNGC 2 cut(s) 80, 604
PleI GAGTC 1 cut(s) 571
PmaCI CACGTG 1 cut(s) 157
PmlI CACGTG 1 cut(s) 157
PpsI GAGTC 1 cut(s) 571
Ppu21I YACGTR 1 cut(s) 157
PsiI TTATAA 2 cut(s) 212, 512
Psp6I CCWGG 1 cut(s) 63
PspCI CACGTG 1 cut(s) 157
PspGI CCWGG 1 cut(s) 63
PspN4I GGNNCC 1 cut(s) 203
PstNI CAGNNNCTG 1 cut(s) 256
PsuI RGATCY 2 cut(s) 146, 215
RsaI GTAC 2 cut(s) 62, 203
RsaNI GTAC 2 cut(s) 61, 202
SapI GCTCTTC 2 cut(s) 39, 612
SaqAI TTAA 2 cut(s) 20, 285
SatI GCNGC 2 cut(s) 79, 603
Sau3AI GATC 6 cut(s) 67, 146, 178, 215, 406, 488
SchI GAGTC 1 cut(s) 572
ScrFI CCNGG 1 cut(s) 65
SfcI CTRYAG 1 cut(s) 456
SfuI TTCGAA 2 cut(s) 582, 726
Sse9I AATT 3 cut(s) 507, 699, 720
SsiI CCGC 3 cut(s) 78, 574, 600
SspI AATATT 1 cut(s) 293
StyD4I CCNGG 1 cut(s) 63
StyI CCWWGG 1 cut(s) 666
TaaI ACNGT 1 cut(s) 460
TaiI ACGT 3 cut(s) 126, 159, 735
TaqI TCGA 4 cut(s) 36, 491, 582, 726
TasI AATT 3 cut(s) 507, 699, 720
TauI GCSGC 1 cut(s) 81
TfiI GAWTC 1 cut(s) 584
Tru1I TTAA 2 cut(s) 20, 285
Tru9I TTAA 2 cut(s) 20, 285
TseFI GTSAC 1 cut(s) 309
TseI GCWGC 1 cut(s) 602
Tsp45I GTSAC 1 cut(s) 309
TspDTI ATGAA 1 cut(s) 373
XapI RAATTY 1 cut(s) 720
XmiI GTMKAC 1 cut(s) 455
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.