RLG00000030694

Folate-sensitive fragile site protein Fra10Ac1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0014301
Physical Location & Seq
Reverse (-)
66020319 .. 66025891
5573 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000030694

Sequence Viewer

Length: 732 bp
ATGGCTTCGTTTGCGTCGTTAAAATCTGAAATATTCGATAGAGAAGAGCGAAAACAGCAGTACCAGGATCACATTCGCGGCCTCAACGCTTACGAACGCCACAAGAAGTTTGTCAAAGATTACGTTAGCTATTATGGTCGAGAAGGATCTTCACACATGAATTTGCCTGTCAAAACTGATCAAGACACCCTAAGAGAAGGGTACCGGTTTATAAGATCTGAGGAAGATGACATGGACACATCTTGGGAGCAGAGGCTGGTGAAGCGATACTATGACAAGCTGTTTAAAGAATATTGCATAGCTGATATGTCACATTACAAGAGTGGTAAGATAGGTCTTAGGTGGAGGACAGAAAAGGAAGTCGTATCCGGTAAAGGGCAGTTCATATGTGGTAGCAAACATTGCAATCTAAAAGATGGCTTAGCCAGCTACGAGGTGAACTTCTCTTATTATGAAGCTGGAGAAAACAAACAAGCACTGGTCAAATTGGTAACTTGTGAGAGATGTTCGGAAAAGCTTCATTACAAACGACGGAAAGAGAAGGAACACATGGAAAAAGAAAAGAATGAAGATAAGAGAAGAAAGAGGAACCGGTCAGGGGATAGTGATGACACTGATGATGAGGGAAGCAAGGAGAGGAGAAAAGGGAAAAAGGCTTCACTTTCCACTAGTGAACAGAGAGTCAATGATGATGAAAACTTTGACGAGTATCTTGAGGGAATGTTTCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Fra10Ac1

Protein Analysis

244

Amino Acids

28.96

Weight (kDa)

8.4

Isoelectric Point (pI)

49.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Fra10Ac1 PF09725 59 - 175 1.2e-50 Folate-sensitive fragile site protein Fra10Ac1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014301)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G15030 AT4G15030 AT4G15030
fragaria_vesca FvH4_7g01110
malus_domestica MD02G1310300.v1.1 MD07G1011200.v1.1
prunus_persica Prupe.2G014400_v2.0.a1
pyrus_communis pycom07g00930
rosa_chinensis RchiOBHm_Chr1g0316111
rosa_laevigata RLG00000030694
rosa_multiflora Rmu_sc0002531.1_g000028
rosa_roxburghii Rroxscaffold_4G00331130
rosa_rugosa Rorug01G0009100
rosa_samantha Rh1AG019100 Rh1BG014100 Rh1CG016700
rosa_wichuraiana Rw1G001240

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 212
Acc65I GGTACC 1 cut(s) 201
AccB1I GGYRCC 1 cut(s) 201
AccII CGCG 1 cut(s) 78
AciI CCGC 1 cut(s) 78
AclWI GGATC 2 cut(s) 75, 154
AcsI RAATTY 1 cut(s) 160
AfaI GTAC 2 cut(s) 62, 203
AfiI CCNNNNNNNGG 2 cut(s) 375, 598
AgeI ACCGGT 2 cut(s) 204, 591
AhlI ACTAGT 1 cut(s) 668
AjnI CCWGG 1 cut(s) 63
AluBI AGCT 6 cut(s) 129, 280, 302, 429, 458, 517
AluI AGCT 6 cut(s) 129, 280, 302, 429, 458, 517
AlwI GGATC 2 cut(s) 75, 154
AlwNI CAGNNNCTG 1 cut(s) 256
AoxI GGCC 1 cut(s) 79
ApoI RAATTY 1 cut(s) 160
AsiGI ACCGGT 2 cut(s) 204, 591
Asp700I GAANNNNTTC 1 cut(s) 516
Asp718I GGTACC 1 cut(s) 201
AsuHPI GGTGA 2 cut(s) 271, 448
BaeI ACNNNNGTAYC 2 cut(s) 44, 77
BanI GGYRCC 1 cut(s) 201
BccI CCATC 1 cut(s) 410
BciT130I CCWGG 1 cut(s) 65
BciVI GTATCC 1 cut(s) 376
BclI TGATCA 1 cut(s) 178
BcuI ACTAGT 1 cut(s) 668
BfaI CTAG 1 cut(s) 669
BfuI GTATCC 1 cut(s) 376
BglII AGATCT 1 cut(s) 215
BisI GCNGC 1 cut(s) 79
BlpI GCTNAGC 1 cut(s) 421
BlsI GCNGC 1 cut(s) 80
Bme1390I CCNGG 1 cut(s) 65
BmiI GGNNCC 2 cut(s) 203, 590
BmrFI CCNGG 1 cut(s) 65
BpmI CTGGAG 1 cut(s) 480
Bpu1102I GCTNAGC 1 cut(s) 421
BsaWI WCCGGW 3 cut(s) 204, 368, 591
Bsc4I CCNNNNNNNGG 2 cut(s) 375, 598
Bse118I RCCGGY 2 cut(s) 204, 591
Bse1I ACTGG 1 cut(s) 483
Bse3DI GCAATG 1 cut(s) 400
BseBI CCWGG 1 cut(s) 65
BseLI CCNNNNNNNGG 2 cut(s) 375, 598
BseMI GCAATG 1 cut(s) 400
BseMII CTCAG 1 cut(s) 210
BseNI ACTGG 1 cut(s) 483
BseRI GAGGAG 1 cut(s) 652
Bsh1236I CGCG 1 cut(s) 78
BshFI GGCC 1 cut(s) 81
BshNI GGYRCC 1 cut(s) 201
BshTI ACCGGT 2 cut(s) 204, 591
BsiSI CCGG 3 cut(s) 205, 369, 592
BslI CCNNNNNNNGG 2 cut(s) 375, 598
BsnI GGCC 1 cut(s) 81
Bsp143I GATC 4 cut(s) 67, 146, 178, 215
Bsp1720I GCTNAGC 1 cut(s) 421
BspACI CCGC 1 cut(s) 78
BspANI GGCC 1 cut(s) 81
BspCNI CTCAG 1 cut(s) 211
BspFNI CGCG 1 cut(s) 78
BspLI GGNNCC 2 cut(s) 203, 590
BspPI GGATC 2 cut(s) 75, 154
BspQI GCTCTTC 1 cut(s) 39
BspT107I GGYRCC 1 cut(s) 201
BsrDI GCAATG 1 cut(s) 400
BsrFI RCCGGY 2 cut(s) 204, 591
BsrI ACTGG 1 cut(s) 483
BssAI RCCGGY 2 cut(s) 204, 591
BssMI GATC 4 cut(s) 67, 146, 178, 215
Bst2UI CCWGG 1 cut(s) 65
Bst6I CTCTTC 1 cut(s) 39
BstAPI GCANNNNNTGC 1 cut(s) 402
BstC8I GCNNGC 1 cut(s) 427
BstDEI CTNAG 4 cut(s) 191, 219, 338, 421
BstFNI CGCG 1 cut(s) 78
BstKTI GATC 4 cut(s) 70, 149, 181, 218
BstMBI GATC 4 cut(s) 67, 146, 178, 215
BstMWI GCNNNNNNNGC 5 cut(s) 11, 55, 262, 402, 426
BstNI CCWGG 1 cut(s) 65
BstSCI CCNGG 1 cut(s) 63
BstUI CGCG 1 cut(s) 78
BstX2I RGATCY 2 cut(s) 146, 215
BstYI RGATCY 2 cut(s) 146, 215
BsuI GTATCC 1 cut(s) 376
BsuRI GGCC 1 cut(s) 81
BtsIMutI CAGTG 2 cut(s) 476, 612
Cac8I GCNNGC 1 cut(s) 427
CaiI CAGNNNCTG 1 cut(s) 256
Cfr10I RCCGGY 2 cut(s) 204, 591
CseI GACGC 1 cut(s) 3
Csp6I GTAC 2 cut(s) 61, 202
CspAI ACCGGT 2 cut(s) 204, 591
CviAII CATG 3 cut(s) 157, 232, 550
CviQI GTAC 2 cut(s) 61, 202
DdeI CTNAG 4 cut(s) 191, 219, 338, 421
DpnI GATC 4 cut(s) 69, 148, 180, 217
DpnII GATC 4 cut(s) 67, 146, 178, 215
DraI TTTAAA 1 cut(s) 286
Eam1104I CTCTTC 1 cut(s) 39
EarI CTCTTC 1 cut(s) 39
EcoRII CCWGG 1 cut(s) 63
FaeI CATG 3 cut(s) 160, 235, 553
FatI CATG 3 cut(s) 156, 231, 549
FauNDI CATATG 1 cut(s) 386
FbaI TGATCA 1 cut(s) 178
Fnu4HI GCNGC 1 cut(s) 79
Fsp4HI GCNGC 1 cut(s) 79
FspBI CTAG 1 cut(s) 669
GluI GCNGC 1 cut(s) 79
GsuI CTGGAG 1 cut(s) 480
HaeIII GGCC 1 cut(s) 81
HapII CCGG 3 cut(s) 205, 369, 592
HgaI GACGC 1 cut(s) 3
Hin1II CATG 3 cut(s) 160, 235, 553
HindIII AAGCTT 1 cut(s) 515
HinfI GANTC 1 cut(s) 681
HpaII CCGG 3 cut(s) 205, 369, 592
HphI GGTGA 2 cut(s) 271, 448
Hpy166II GTNNAC 2 cut(s) 439, 674
Hpy188I TCNGA 3 cut(s) 28, 220, 511
Hpy188III TCNNGA 3 cut(s) 140, 182, 713
Hpy8I GTNNAC 2 cut(s) 439, 674
Hpy99I CGWCG 2 cut(s) 19, 534
HpyAV CCTTC 3 cut(s) 137, 191, 535
HpyCH4IV ACGT 1 cut(s) 123
HpyCH4V TGCA 2 cut(s) 297, 405
HpyF10VI GCNNNNNNNGC 5 cut(s) 11, 55, 262, 402, 426
HpyF3I CTNAG 4 cut(s) 191, 219, 338, 421
HpySE526I ACGT 1 cut(s) 123
Hsp92II CATG 3 cut(s) 160, 235, 553
KpnI GGTACC 1 cut(s) 205
Ksp22I TGATCA 1 cut(s) 178
Kzo9I GATC 4 cut(s) 67, 146, 178, 215
LguI GCTCTTC 1 cut(s) 39
LmnI GCTCC 1 cut(s) 247
MaeI CTAG 1 cut(s) 669
MaeII ACGT 1 cut(s) 123
MaeIII GTNAC 2 cut(s) 309, 490
MalI GATC 4 cut(s) 69, 148, 180, 217
MboI GATC 4 cut(s) 67, 146, 178, 215
MboII GAAGA 5 cut(s) 56, 141, 236, 581, 591
MflI RGATCY 2 cut(s) 146, 215
MluCI AATT 2 cut(s) 160, 485
MlyI GAGTC 1 cut(s) 690
MnlI CCTC 9 cut(s) 92, 214, 246, 339, 427, 579, 616, 630, 709
MroXI GAANNNNTTC 1 cut(s) 516
MseI TTAA 2 cut(s) 20, 285
MspI CCGG 3 cut(s) 205, 369, 592
MspR9I CCNGG 1 cut(s) 65
MvaI CCWGG 1 cut(s) 65
MvnI CGCG 1 cut(s) 78
MwoI GCNNNNNNNGC 5 cut(s) 11, 55, 262, 402, 426
NdeI CATATG 1 cut(s) 386
NdeII GATC 4 cut(s) 67, 146, 178, 215
NlaIII CATG 3 cut(s) 160, 235, 553
NlaIV GGNNCC 2 cut(s) 203, 590
NmuCI GTSAC 1 cut(s) 309
PciSI GCTCTTC 1 cut(s) 39
PcsI WCGNNNNNNNCGW 1 cut(s) 14
PdmI GAANNNNTTC 1 cut(s) 516
PinAI ACCGGT 2 cut(s) 204, 591
PkrI GCNGC 1 cut(s) 80
PleI GAGTC 1 cut(s) 689
PpsI GAGTC 1 cut(s) 689
PsiI TTATAA 1 cut(s) 212
Psp6I CCWGG 1 cut(s) 63
PspGI CCWGG 1 cut(s) 63
PspN4I GGNNCC 2 cut(s) 203, 590
PstNI CAGNNNCTG 1 cut(s) 256
PsuI RGATCY 2 cut(s) 146, 215
RsaI GTAC 2 cut(s) 62, 203
RsaNI GTAC 2 cut(s) 61, 202
SapI GCTCTTC 1 cut(s) 39
SaqAI TTAA 2 cut(s) 20, 285
SatI GCNGC 1 cut(s) 79
Sau3AI GATC 4 cut(s) 67, 146, 178, 215
SchI GAGTC 1 cut(s) 690
ScrFI CCNGG 1 cut(s) 65
SmlI CTYRAG 1 cut(s) 713
SmoI CTYRAG 1 cut(s) 713
SpeI ACTAGT 1 cut(s) 668
Sse9I AATT 2 cut(s) 160, 485
SsiI CCGC 1 cut(s) 78
SspI AATATT 2 cut(s) 33, 293
SspMI CTAG 1 cut(s) 669
StyD4I CCNGG 1 cut(s) 63
TaiI ACGT 1 cut(s) 126
TaqI TCGA 2 cut(s) 36, 139
TasI AATT 2 cut(s) 160, 485
TauI GCSGC 1 cut(s) 81
Tru1I TTAA 2 cut(s) 20, 285
Tru9I TTAA 2 cut(s) 20, 285
TscAI CASTG 2 cut(s) 483, 619
TseFI GTSAC 1 cut(s) 309
Tsp45I GTSAC 1 cut(s) 309
TspDTI ATGAA 6 cut(s) 173, 373, 468, 509, 582, 708
TspGWI ACGGA 1 cut(s) 547
TspRI CASTG 2 cut(s) 483, 619
XapI RAATTY 1 cut(s) 160
XmnI GAANNNNTTC 1 cut(s) 516
XspI CTAG 1 cut(s) 669
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.