Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr07

Orthogroup ID

OG0007211

Physical Location & Seq

Genomic Coordinates Reverse (-)

5723683 .. 5728210

Gene Structure 4528 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD07G1061200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1602 bp

ATGAACGCCCCAATTATCGACCCATTACAAGGGGATTTCCCAGAAGTGATAGAGGAGTATTTGGAGCATGGCGTTATGAAATGCATCGCCTTCAACCGCCGCGGTACCCTTCTGGCTGCGGGGTGCTCTGACGGAAATTGCATCATATGGGATTTTGAGACCAGGGGCATTGCCAAGGAGCTTCGTGATAAAGATTGTGTTGCGGCGATAACTAGTGTATGTTGGTCAAAGTATGGCCATTGCATTCTTGTGTCTGCTGCGGACAAGTCGTTGACACTATGGGATGTTGTCAGTGGAGAGAAGATAACGCGAACAGTACTGCAACAAACCCCTTTACAAGCTCGGCTTCATCCTGGTTCGCCTACTCCATCTCTTTGCCTGGCATGCCCTCTCTCTTCTGCTCCCATCATTGTTGACTTGAATACTGGAAGCACAGTTGTTCTTCCAGTTTCCATTCCTGACTCTAACCCAGGGCTTACCCCTCTGTCACGGAACAAAATCTCTGAGGGAACTCCACCTTTCTCTCCTACTGCTGCTTGCTTTAATAAGCATGGTGATCTAGTATATGCGGGGAACTCCAAAGGTGAAATACTTGTAATAGATTATAAAAGTGTTCAAGTGCGTGCCGTGGTTCCCATTTCTGGAGGTTCTGTGATTAAGAACATAGTATTCAGCAGGAATGGACAGTATTTGCTTACAAATTCAAATGATAGAACAATCAGGATCTACGAGAACCTACTGCCTCTGAAAGATGGACTTAAAACCTTGGATGACCTAAATGAGACCCTTGATGGAATTGATGGTGTTGAGAAGTTGAAAGCTGTTGGATCAAAATGCTTAACGCTTTTCCGTGAGTTTCAAGATGCCATCACAAAGATGCATTGGAAAGCACCTTGTTTCAGTGGTGATGGTGAGTGGGTGATTGGTGGTTCTGCAAGCAAAGGAGAACACAAGATTTACATATGGGATAGGGCTGGGCATCTTGTGAAAATTCTTGAAGGGCCAAAGGAAGCCTTGATCGATTTGGTGTGGCATCCTGTTCACCCCATTGTTGTCTCTGTTTCACTGACTGGTTTGGTTTATATCTGGGCTAAAGATTACACTGAGAACTGGAGTGCATTTGCTCCTGATTTCAAAGAGCTTGAGGAAAATGAGGAATACATAGAACGAGAGGATGAGTTTGATCTAATCCCTGAAACTGAGAAGGTAAAAGGATCTGATATTAATGAAGATGATGAAGTTGATATTGTGACAGTGGAGAAGGATTCAGCTTTCAGTGATTCAGATATGTCACAGGAAGAGCTTTGCTTTTTACCTGCAATTCCTTCTCCTGATGTTCCGGACCAACAAGATAAGTGCATAGAGAGTTCATCAAAGTTGGTGGATAGTAATTCTGGCTCCCCTATTTCAGAAGAGGGCACACCAAATGGATGTGCAATTGATCATGCATCAAGTCCACTTGAAGGATGCTGGAGCAACACGCTTGAAAAGAAAACGGAAGCCTTCCGAGAAGGGGTTGGAGTTTCAGGCTTAGAAGTTTCAAATACAGCAGCGATGACAACGGTTGATGCTGCTAGGTATGACCATTCTCTTGCATCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0001067 GO:0003006 GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005730 GO:0006325 GO:0006464 GO:0006479 GO:0006807 GO:0006950 GO:0006974 GO:0006996 GO:0007275 GO:0008150 GO:0008152 GO:0008213 GO:0009791 GO:0009987 GO:0010228 GO:0016043 GO:0016569 GO:0016570 GO:0016571 GO:0018022 GO:0018193 GO:0018205 GO:0019538 GO:0022414 GO:0031974 GO:0031981 GO:0032259 GO:0032501 GO:0032502 GO:0032991 GO:0033554 GO:0034708 GO:0034968 GO:0035064 GO:0035097 GO:0036211 GO:0042393 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0043414 GO:0043627 GO:0044212 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0044665 GO:0044666 GO:0048188 GO:0048608 GO:0048731 GO:0048856 GO:0050896 GO:0051276 GO:0051568 GO:0051716 GO:0061458 GO:0070013 GO:0071339 GO:0071704 GO:0071840 GO:0097159 GO:0140030 GO:0140034 GO:1901363 GO:1901564 GO:1902494 GO:1990234

KEGG Pathways

Metabolic & Signaling

ko04934 map04934

Pfam Domains

Protein Families

WD40

Protein Analysis

534

Amino Acids

58.09

Weight (kDa)

4.66

Isoelectric Point (pI)

37.05

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Beta-prop_EML	PF23409	17 - 109	8.8e-06	Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_TEP1_2nd	PF25047	17 - 111	7e-07	TEP-1 second beta-propeller
Beta-prop_EML_2	PF23414	25 - 104	6.3e-08	Echinoderm microtubule-associated protein second beta-propeller
WD40_Prp19	PF24814	25 - 100	3.5e-08	Prp19 WD40 domain
Beta-prop_WDR5	PF25175	26 - 104	5.2e-12	WDR5 beta-propeller domain
Beta-prop_THOC3	PF25174	26 - 118	1.7e-11	THOC3 beta-propeller domain
Beta-prop_WDR3_1st	PF25173	26 - 104	2.1e-09	WDR3 first beta-propeller domain
Beta-prop_WDR36-Utp21_2nd	PF25168	26 - 111	2.7e-06	WDR36/Utp21 second beta-propeller domain
EIF3I	PF24805	29 - 244	9.7e-07	EIF3I
Beta-prop_WDR36-Utp21_1st	PF25171	35 - 243	1e-05	WDR36/Utp21 first beta-propeller
Beta-prop_EML_2	PF23414	177 - 244	1.5e-06	Echinoderm microtubule-associated protein second beta-propeller

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0007211)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G21060
fragaria_vesca	FvH4_2g10510 FvH4_2g10510 FvH4_2g10510 FvH4_2g10510
malus_domestica	MD02G1259400.v1.1 MD07G1061200.v1.1
prunus_persica	Prupe.5G058900_v2.0.a1 Prupe.5G058900_v2.0.a1
pyrus_communis	pycom02g22080
rosa_chinensis	RchiOBHm_Chr6g0268021
rosa_laevigata	RLG00000013939
rosa_multiflora	Rmu_sc0001839.1_g000031 Rmu_sc0003304.1_g000001 Rmu_sc0003304.1_g000039 Rmu_sc0004529.1_g000038
rosa_roxburghii	Rroxscaffold_7G00200120 Rroxscaffold_7G00200140
rosa_rugosa	Rorug06G0038800
rosa_samantha	Rh6AG158100 Rh6BG162100 Rh6CG156300 Rh6DG147700
rosa_wichuraiana	Rw0G021300 Rw6G013760

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	606
Acc36I	ACCTGC	1 cut(s)	1324
Acc65I	GGTACC	1 cut(s)	104
AccB1I	GGYRCC	1 cut(s)	104
AccII	CGCG	2 cut(s)	102, 310
AccIII	TCCGGA	1 cut(s)	1339
AciI	CCGC	7 cut(s)	97, 100, 102, 119, 203, 260, 569
AclWI	GGATC	3 cut(s)	731, 835, 1222
AcoI	YGGCCR	1 cut(s)	235
AcsI	RAATTY	2 cut(s)	700, 990
AfaI	GTAC	2 cut(s)	106, 318
AfiI	CCNNNNNNNGG	4 cut(s)	29, 641, 1463, 1513
AhlI	ACTAGT	1 cut(s)	212
AjnI	CCWGG	4 cut(s)	161, 352, 378, 469
AluBI	AGCT	6 cut(s)	181, 341, 821, 1141, 1271, 1303
AluI	AGCT	6 cut(s)	181, 341, 821, 1141, 1271, 1303
Alw21I	GWGCWC	1 cut(s)	128
Alw26I	GTCTC	3 cut(s)	152, 776, 1060
AlwI	GGATC	3 cut(s)	731, 835, 1222
Aor13HI	TCCGGA	1 cut(s)	1339
AoxI	GGCC	2 cut(s)	235, 1001
ApeKI	GCWGC	5 cut(s)	116, 257, 533, 1550, 1571
ApoI	RAATTY	2 cut(s)	700, 990
AseI	ATTAAT	1 cut(s)	1224
Asp718I	GGTACC	1 cut(s)	104
AspS9I	GGNCC	2 cut(s)	1001, 1342
AsuHPI	GGTGA	6 cut(s)	566, 596, 917, 923, 931, 1034
AvaII	GGWCC	1 cut(s)	1342
BaeGI	GKGCMC	1 cut(s)	1421
BalI	TGGCCA	1 cut(s)	237
BanI	GGYRCC	1 cut(s)	104
Bbv12I	GWGCWC	1 cut(s)	128
BbvI	GCAGC	5 cut(s)	103, 244, 520, 1558, 1562
BccI	CCATC	7 cut(s)	376, 413, 746, 785, 794, 875, 902
BceAI	ACGGC	1 cut(s)	611
BciT130I	CCWGG	4 cut(s)	163, 354, 380, 471
BclI	TGATCA	1 cut(s)	1441
BcoDI	GTCTC	3 cut(s)	152, 776, 1060
BcuI	ACTAGT	1 cut(s)	212
BfaI	CTAG	3 cut(s)	213, 560, 1575
BfuAI	ACCTGC	1 cut(s)	1324
BisI	GCNGC	7 cut(s)	100, 117, 204, 258, 534, 1551, 1572
BlsI	GCNGC	7 cut(s)	101, 118, 205, 259, 535, 1552, 1573
BmcAI	AGTACT	1 cut(s)	318
Bme1390I	CCNGG	4 cut(s)	163, 354, 380, 471
Bme18I	GGWCC	1 cut(s)	1342
BmgT120I	GGNCC	2 cut(s)	1001, 1342
BmiI	GGNNCC	3 cut(s)	106, 633, 1399
BmrFI	CCNGG	4 cut(s)	163, 354, 380, 471
BmsI	GCATC	9 cut(s)	93, 150, 853, 867, 988, 1042, 1457, 1457, 1558
BpmI	CTGGAG	3 cut(s)	663, 1132, 1492
BpuEI	CTTGAG	1 cut(s)	1163
Bsa29I	ATCGAT	1 cut(s)	1020
BsaI	GGTCTC	2 cut(s)	152, 776
BsaJI	CCNNGG	7 cut(s)	100, 162, 174, 469, 470, 627, 765
BsaWI	WCCGGW	1 cut(s)	1339
BsaXI	ACNNNNNCTCC	2 cut(s)	636, 666
Bsc4I	CCNNNNNNNGG	4 cut(s)	29, 641, 1463, 1513
Bse1I	ACTGG	4 cut(s)	430, 446, 1075, 1115
Bse3DI	GCAATG	2 cut(s)	168, 238
BseAI	TCCGGA	1 cut(s)	1339
BseBI	CCWGG	4 cut(s)	163, 354, 380, 471
BseCI	ATCGAT	1 cut(s)	1020
BseDI	CCNNGG	7 cut(s)	100, 162, 174, 469, 470, 627, 765
BseGI	GGATG	7 cut(s)	289, 349, 775, 1033, 1180, 1436, 1472
BseLI	CCNNNNNNNGG	4 cut(s)	29, 641, 1463, 1513
BseMI	GCAATG	2 cut(s)	168, 238
BseMII	CTCAG	3 cut(s)	495, 1095, 1191
BseNI	ACTGG	4 cut(s)	430, 446, 1075, 1115
BseRI	GAGGAG	1 cut(s)	68
BseSI	GKGCMC	1 cut(s)	1421
BseXI	GCAGC	5 cut(s)	103, 244, 520, 1558, 1562
BseYI	CCCAGC	1 cut(s)	974
Bsh1236I	CGCG	2 cut(s)	102, 310
BshFI	GGCC	2 cut(s)	237, 1003
BshNI	GGYRCC	1 cut(s)	104
BshVI	ATCGAT	1 cut(s)	1020
BsiHKAI	GWGCWC	1 cut(s)	128
BsiSI	CCGG	1 cut(s)	1340
BslI	CCNNNNNNNGG	4 cut(s)	29, 641, 1463, 1513
BsmAI	GTCTC	3 cut(s)	152, 776, 1060
BsmI	GAATGC	1 cut(s)	243
BsnI	GGCC	2 cut(s)	237, 1003
Bso31I	GGTCTC	2 cut(s)	152, 776
Bsp1286I	GDGCHC	2 cut(s)	128, 1421
Bsp13I	TCCGGA	1 cut(s)	1339
Bsp143I	GATC	7 cut(s)	556, 723, 827, 1017, 1183, 1214, 1441
BspACI	CCGC	7 cut(s)	97, 100, 102, 119, 203, 260, 569
BspANI	GGCC	2 cut(s)	237, 1003
BspCNI	CTCAG	3 cut(s)	496, 1096, 1192
BspDI	ATCGAT	1 cut(s)	1020
BspEI	TCCGGA	1 cut(s)	1339
BspFNI	CGCG	2 cut(s)	102, 310
BspLI	GGNNCC	3 cut(s)	106, 633, 1399
BspMI	ACCTGC	1 cut(s)	1324
BspPI	GGATC	3 cut(s)	731, 835, 1222
BspQI	GCTCTTC	1 cut(s)	1293
BspT107I	GGYRCC	1 cut(s)	104
BspTNI	GGTCTC	2 cut(s)	152, 776
BsrDI	GCAATG	2 cut(s)	168, 238
BsrI	ACTGG	4 cut(s)	430, 446, 1075, 1115
BssECI	CCNNGG	7 cut(s)	100, 162, 174, 469, 470, 627, 765
BssMI	GATC	7 cut(s)	556, 723, 827, 1017, 1183, 1214, 1441
BssT1I	CCWWGG	2 cut(s)	174, 765
Bst2UI	CCWGG	4 cut(s)	163, 354, 380, 471
Bst4CI	ACNGT	5 cut(s)	316, 436, 687, 1255, 1564
Bst6I	CTCTTC	3 cut(s)	400, 1293, 1407
BstC8I	GCNNGC	4 cut(s)	385, 538, 624, 937
BstDEI	CTNAG	4 cut(s)	504, 1104, 1200, 1531
BstDSI	CCRYGG	2 cut(s)	100, 627
BstF5I	GGATG	7 cut(s)	289, 349, 775, 1033, 1180, 1436, 1472
BstFNI	CGCG	2 cut(s)	102, 310
BstKTI	GATC	7 cut(s)	559, 726, 830, 1020, 1186, 1217, 1444
BstMAI	GTCTC	3 cut(s)	152, 776, 1060
BstMBI	GATC	7 cut(s)	556, 723, 827, 1017, 1183, 1214, 1441
BstMWI	GCNNNNNNNGC	1 cut(s)	384
BstNI	CCWGG	4 cut(s)	163, 354, 380, 471
BstNSI	RCATGY	1 cut(s)	387
BstSCI	CCNGG	4 cut(s)	161, 352, 378, 469
BstSLI	GKGCMC	1 cut(s)	1421
BstUI	CGCG	2 cut(s)	102, 310
BstV1I	GCAGC	5 cut(s)	103, 244, 520, 1558, 1562
BstX2I	RGATCY	2 cut(s)	723, 1214
BstYI	RGATCY	2 cut(s)	723, 1214
Bsu15I	ATCGAT	1 cut(s)	1020
BsuRI	GGCC	2 cut(s)	237, 1003
BsuTUI	ATCGAT	1 cut(s)	1020
BtgI	CCRYGG	2 cut(s)	100, 627
BtgZI	GCGATG	2 cut(s)	70, 1568
BtsCI	GGATG	7 cut(s)	289, 349, 775, 1033, 1180, 1436, 1472
BtsIMutI	CAGTG	6 cut(s)	298, 907, 1064, 1101, 1260, 1282
BveI	ACCTGC	1 cut(s)	1324
Cac8I	GCNNGC	4 cut(s)	385, 538, 624, 937
Cfr13I	GGNCC	2 cut(s)	1001, 1342
Cfr42I	CCGCGG	1 cut(s)	103
ClaI	ATCGAT	1 cut(s)	1020
Csp6I	GTAC	2 cut(s)	105, 317
CspCI	CAANNNNNGTGG	2 cut(s)	1362, 1397
CviAII	CATG	4 cut(s)	68, 384, 551, 1445
CviQI	GTAC	2 cut(s)	105, 317
DdeI	CTNAG	4 cut(s)	504, 1104, 1200, 1531
DpnI	GATC	7 cut(s)	558, 725, 829, 1019, 1185, 1216, 1443
DpnII	GATC	7 cut(s)	556, 723, 827, 1017, 1183, 1214, 1441
EaeI	YGGCCR	1 cut(s)	235
Eam1104I	CTCTTC	3 cut(s)	400, 1293, 1407
EarI	CTCTTC	3 cut(s)	400, 1293, 1407
Eco130I	CCWWGG	2 cut(s)	174, 765
Eco31I	GGTCTC	2 cut(s)	152, 776
Eco47I	GGWCC	1 cut(s)	1342
EcoRII	CCWGG	4 cut(s)	161, 352, 378, 469
EcoT14I	CCWWGG	2 cut(s)	174, 765
EcoT22I	ATGCAT	3 cut(s)	86, 882, 1450
ErhI	CCWWGG	2 cut(s)	174, 765
FaeI	CATG	4 cut(s)	71, 387, 554, 1448
FalI	AAGNNNNNCTT	6 cut(s)	330, 362, 741, 773, 998, 1030
FatI	CATG	4 cut(s)	67, 383, 550, 1444
FauI	CCCGC	2 cut(s)	112, 562
FauNDI	CATATG	2 cut(s)	146, 962
FbaI	TGATCA	1 cut(s)	1441
Fnu4HI	GCNGC	7 cut(s)	100, 117, 204, 258, 534, 1551, 1572
FokI	GGATG	7 cut(s)	296, 336, 782, 1020, 1187, 1443, 1479
Fsp4HI	GCNGC	7 cut(s)	100, 117, 204, 258, 534, 1551, 1572
FspBI	CTAG	3 cut(s)	213, 560, 1575
GluI	GCNGC	7 cut(s)	100, 117, 204, 258, 534, 1551, 1572
GsaI	CCCAGC	1 cut(s)	978
GsuI	CTGGAG	3 cut(s)	663, 1132, 1492
HaeIII	GGCC	2 cut(s)	237, 1003
HapII	CCGG	1 cut(s)	1340
Hin1II	CATG	4 cut(s)	71, 387, 554, 1448
HincII	GTYRAC	2 cut(s)	273, 415
HindII	GTYRAC	2 cut(s)	273, 415
HinfI	GANTC	3 cut(s)	461, 1265, 1280
HpaII	CCGG	1 cut(s)	1340
HphI	GGTGA	6 cut(s)	566, 596, 917, 923, 931, 1034
Hpy166II	GTNNAC	4 cut(s)	273, 415, 1042, 1457
Hpy188I	TCNGA	7 cut(s)	130, 505, 747, 1219, 1285, 1411, 1508
Hpy8I	GTNNAC	4 cut(s)	273, 415, 1042, 1457
HpyAV	CCTTC	9 cut(s)	100, 119, 992, 1198, 1255, 1335, 1457, 1505, 1513
HpyCH4III	ACNGT	5 cut(s)	316, 436, 687, 1255, 1564
HpyF10VI	GCNNNNNNNGC	1 cut(s)	384
HpyF3I	CTNAG	4 cut(s)	504, 1104, 1200, 1531
Hsp92II	CATG	4 cut(s)	71, 387, 554, 1448
Kpn2I	TCCGGA	1 cut(s)	1339
KpnI	GGTACC	1 cut(s)	108
Ksp22I	TGATCA	1 cut(s)	1441
KspI	CCGCGG	1 cut(s)	103
Kzo9I	GATC	7 cut(s)	556, 723, 827, 1017, 1183, 1214, 1441
LguI	GCTCTTC	1 cut(s)	1293
LmnI	GCTCC	6 cut(s)	64, 178, 406, 1129, 1403, 1473
Lsp1109I	GCAGC	5 cut(s)	103, 244, 520, 1558, 1562
LweI	GCATC	9 cut(s)	93, 150, 853, 867, 988, 1042, 1457, 1457, 1558
MaeI	CTAG	3 cut(s)	213, 560, 1575
MaeIII	GTNAC	3 cut(s)	486, 1249, 1290
MalI	GATC	7 cut(s)	558, 725, 829, 1019, 1185, 1216, 1443
MboI	GATC	7 cut(s)	556, 723, 827, 1017, 1183, 1214, 1441
MboII	GAAGA	6 cut(s)	313, 387, 434, 1241, 1310, 1424
MfeI	CAATTG	1 cut(s)	1437
MflI	RGATCY	2 cut(s)	723, 1214
MhlI	GDGCHC	2 cut(s)	128, 1421
MlsI	TGGCCA	1 cut(s)	237
MluCI	AATT	8 cut(s)	12, 136, 700, 795, 990, 1320, 1390, 1437
MluNI	TGGCCA	1 cut(s)	237
MlyI	GAGTC	1 cut(s)	455
MmeI	TCCRAC	2 cut(s)	805, 1498
Mox20I	TGGCCA	1 cut(s)	237
Mph1103I	ATGCAT	3 cut(s)	86, 882, 1450
MroI	TCCGGA	1 cut(s)	1339
MscI	TGGCCA	1 cut(s)	237
MseI	TTAA	5 cut(s)	543, 657, 759, 839, 1224
MslI	CAYNNNNRTG	2 cut(s)	248, 875
Msp20I	TGGCCA	1 cut(s)	237
MspA1I	CMGCKG	1 cut(s)	102
MspI	CCGG	1 cut(s)	1340
MspR9I	CCNGG	4 cut(s)	163, 354, 380, 471
MunI	CAATTG	1 cut(s)	1437
Mva1269I	GAATGC	1 cut(s)	243
MvaI	CCWGG	4 cut(s)	163, 354, 380, 471
MvnI	CGCG	2 cut(s)	102, 310
MwoI	GCNNNNNNNGC	1 cut(s)	384
NdeI	CATATG	2 cut(s)	146, 962
NdeII	GATC	7 cut(s)	556, 723, 827, 1017, 1183, 1214, 1441
NlaIII	CATG	4 cut(s)	71, 387, 554, 1448
NlaIV	GGNNCC	3 cut(s)	106, 633, 1399
NmeAIII	GCCGAG	1 cut(s)	322
NmuCI	GTSAC	3 cut(s)	486, 1249, 1290
NsiI	ATGCAT	3 cut(s)	86, 882, 1450
NspI	RCATGY	1 cut(s)	387
PaeI	GCATGC	1 cut(s)	387
PasI	CCCWGGG	1 cut(s)	470
PciSI	GCTCTTC	1 cut(s)	1293
PctI	GAATGC	1 cut(s)	243
PfeI	GAWTC	2 cut(s)	1265, 1280
PkrI	GCNGC	7 cut(s)	101, 118, 205, 259, 535, 1552, 1573
PleI	GAGTC	1 cut(s)	455
PpsI	GAGTC	1 cut(s)	455
PshBI	ATTAAT	1 cut(s)	1224
PsiI	TTATAA	1 cut(s)	606
Psp6I	CCWGG	4 cut(s)	161, 352, 378, 469
PspFI	CCCAGC	1 cut(s)	974
PspGI	CCWGG	4 cut(s)	161, 352, 378, 469
PspN4I	GGNNCC	3 cut(s)	106, 633, 1399
PspPI	GGNCC	2 cut(s)	1001, 1342
PsuI	RGATCY	2 cut(s)	723, 1214
RsaI	GTAC	2 cut(s)	106, 318
RsaNI	GTAC	2 cut(s)	105, 317
RseI	CAYNNNNRTG	2 cut(s)	248, 875
SacII	CCGCGG	1 cut(s)	103
SapI	GCTCTTC	1 cut(s)	1293
SaqAI	TTAA	5 cut(s)	543, 657, 759, 839, 1224
SatI	GCNGC	7 cut(s)	100, 117, 204, 258, 534, 1551, 1572
Sau3AI	GATC	7 cut(s)	556, 723, 827, 1017, 1183, 1214, 1441
Sau96I	GGNCC	2 cut(s)	1001, 1342
ScaI	AGTACT	1 cut(s)	318
SchI	GAGTC	1 cut(s)	455
ScrFI	CCNGG	4 cut(s)	163, 354, 380, 471
SduI	GDGCHC	2 cut(s)	128, 1421
SfaNI	GCATC	9 cut(s)	93, 150, 853, 867, 988, 1042, 1457, 1457, 1558
Sfr303I	CCGCGG	1 cut(s)	103
SgrBI	CCGCGG	1 cut(s)	103
SinI	GGWCC	1 cut(s)	1342
SmiMI	CAYNNNNRTG	2 cut(s)	248, 875
SmlI	CTYRAG	1 cut(s)	1142
SmoI	CTYRAG	1 cut(s)	1142
SpeI	ACTAGT	1 cut(s)	212
SphI	GCATGC	1 cut(s)	387
Sse9I	AATT	8 cut(s)	12, 136, 700, 795, 990, 1320, 1390, 1437
SsiI	CCGC	7 cut(s)	97, 100, 102, 119, 203, 260, 569
SspMI	CTAG	3 cut(s)	213, 560, 1575
StyD4I	CCNGG	4 cut(s)	161, 352, 378, 469
StyI	CCWWGG	2 cut(s)	174, 765
TaaI	ACNGT	5 cut(s)	316, 436, 687, 1255, 1564
TaqI	TCGA	2 cut(s)	18, 1020
TasI	AATT	8 cut(s)	12, 136, 700, 795, 990, 1320, 1390, 1437
TatI	WGTACW	1 cut(s)	316
TauI	GCSGC	2 cut(s)	102, 206
TfiI	GAWTC	2 cut(s)	1265, 1280
Tru1I	TTAA	5 cut(s)	543, 657, 759, 839, 1224
Tru9I	TTAA	5 cut(s)	543, 657, 759, 839, 1224
TscAI	CASTG	6 cut(s)	298, 907, 1071, 1108, 1260, 1282
TseFI	GTSAC	3 cut(s)	486, 1249, 1290
TseI	GCWGC	5 cut(s)	116, 257, 533, 1550, 1571
Tsp45I	GTSAC	3 cut(s)	486, 1249, 1290
TspDTI	ATGAA	6 cut(s)	17, 92, 338, 1242, 1251, 1359
TspGWI	ACGGA	4 cut(s)	147, 505, 839, 1511
TspRI	CASTG	6 cut(s)	298, 907, 1071, 1108, 1260, 1282
VpaK11BI	GGWCC	1 cut(s)	1342
VspI	ATTAAT	1 cut(s)	1224
XapI	RAATTY	2 cut(s)	700, 990
XceI	RCATGY	1 cut(s)	387
XspI	CTAG	3 cut(s)	213, 560, 1575
ZrmI	AGTACT	1 cut(s)	318
Zsp2I	ATGCAT	3 cut(s)	86, 882, 1450

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.