Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr6B

Orthogroup ID

OG0007211

Physical Location & Seq

Genomic Coordinates Reverse (-)

26364317 .. 26407625

Gene Structure 43309 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh6BG162100.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1536 bp

ATGAACGCTTCAATAATCGACCCACTGCAAGGAGATTTCCCAGAAGTGATAGAGGAGTATTTGGAACATGGGGTTATGAAATGCATTGCCTTCAATCGCCGTGGTACTCTTCTTGCTGCTGGGTGCTCTGATGGTAATTGCATAATATGGGATTTTGAGACCAGGGGCATTGCTAAGGAGCTCCGTGATAAAGACTGTGTTGCTGCCATAACTAGTGTATGTTGGTCAAAGTATGGTCATCGCGTTCTTGTGTCTGCAGCTGACAAATCGTTGACACTATGGGACGTTGTTAGTGGTGAGAAGATAACAAGAACGATACTGCAACAAACCCCTCTACAAGCTCGGTTACATCCTGGTTCCTCTACTCCATCTCTCTGCCTGGCTTGCCCTCTCTCATCTGCTCCCATGATTGTTGACTTGAATACAGGAAGTGCAACTATGCTTCCAGTTTCCATTCCGGACTCTAACCCAGTACTTGCCCCTCCATCGCGGAATAAAATCTCTGATGGAACTCCCCCCTTTTCTCCTACTGCTGCTTGCTTTAATAAGTATGGGGATCTGGTTTATGCAGGGAACTCGAAAGGAGAAATACTTCTAATAGATTATAAAACTGTCCAAGTGCTTGCCATGGTTCCTACTTCTGGAGGTTCCGTGATTAAGAATATAGTATTCAGCAGGAATGGTCAGTATCTGCTTACAAATTCAAATGATAGAACAATAAGGATTTACGAGAACCTTCTGCCCTTGAAAGATGGACTGAAAGCCCTAAATGGCTTAACTGCTCTTGATGGACTAGATGATGCTGAGAAGTTGAAAGTTGTTGGATCAAAATGCTTAACACTATTCCGTGAGTTTCAAGATACCATCACAAAGATGCATTGGAAAGCACCTTGTTTCAGTGGTGATGGTGAGTGGGTAATTGGTGGTTCTGCAAGCAAAGGAGAACACAAGATTTACATATGGGACAGGGCTGGGCATCTTGTGAAAATTCTTGAAGGGCCAAAGGAAGCCTTGATCGATTTAGTGTGGCATCCTGTTCATCCCATTGTTGTCTCTGTTTCCTTGACGGGCTTGGTTTATATTTGGGCTAAAGATTACACTGAGAACTGGAGTGCATTTGCTCCTGATTTTAAGGAGCTCGAGGAAAATGAGGAGTACATAGAACGAGAAGATGAATTTGATCTGATACCTGAAACTGAAAAGGCCAAAGAATCTAATGTTAATGAAGATGATGAAGTTGATATCGTGACAGTGGACAAGGATTCAGCTTTCAGTGATTCAGATATGTCACAGGAAGAGCTATGCTTCTTGCCAGCAATTCCTTTGCCTGATGCTCCGGAGCAACAAGACAAGTGCTTAGAAAGTTCATCAAAGTTGGTGGAAAGTAATAATTCTGGATCCCCTCTTTCAGAAGAGGGCAGACCAAATGGACGTGCAATGAATCATGCATCAAGTCCGCTTGAAGAGGATCCTGGAGCAACACGCTTGAAAAGAAAATGGAAGCCTTCTGAAAAGTGGTTGGACTTGCAGCTTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0001067 GO:0003006 GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005730 GO:0006325 GO:0006464 GO:0006479 GO:0006807 GO:0006950 GO:0006974 GO:0006996 GO:0007275 GO:0008150 GO:0008152 GO:0008213 GO:0009791 GO:0009987 GO:0010228 GO:0016043 GO:0016569 GO:0016570 GO:0016571 GO:0018022 GO:0018193 GO:0018205 GO:0019538 GO:0022414 GO:0031974 GO:0031981 GO:0032259 GO:0032501 GO:0032502 GO:0032991 GO:0033554 GO:0034708 GO:0034968 GO:0035064 GO:0035097 GO:0036211 GO:0042393 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0043414 GO:0043627 GO:0044212 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0044665 GO:0044666 GO:0048188 GO:0048608 GO:0048731 GO:0048856 GO:0050896 GO:0051276 GO:0051568 GO:0051716 GO:0061458 GO:0070013 GO:0071339 GO:0071704 GO:0071840 GO:0097159 GO:0140030 GO:0140034 GO:1901363 GO:1901564 GO:1902494 GO:1990234

KEGG Pathways

Metabolic & Signaling

ko04934 map04934

Pfam Domains

Protein Families

WD40

Protein Analysis

511

Amino Acids

56.3

Weight (kDa)

4.84

Isoelectric Point (pI)

40.95

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Beta-prop_TEP1_2nd	PF25047	14 - 111	8.1e-07	TEP-1 second beta-propeller
Beta-prop_WDR5	PF25175	25 - 105	2e-11	WDR5 beta-propeller domain
Beta-prop_WDR3_1st	PF25173	25 - 104	8.3e-10	WDR3 first beta-propeller domain
WD40_Prp19	PF24814	25 - 100	5e-08	Prp19 WD40 domain
Beta-prop_THOC3	PF25174	26 - 118	5e-12	THOC3 beta-propeller domain
Beta-prop_EML_2	PF23414	26 - 104	2.8e-07	Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR36-Utp21_2nd	PF25168	26 - 110	4.8e-06	WDR36/Utp21 second beta-propeller domain
EIF3I	PF24805	28 - 244	4.2e-08	EIF3I
Beta-prop_WDR36-Utp21_1st	PF25171	35 - 243	7e-06	WDR36/Utp21 first beta-propeller
Beta-prop_WDR5	PF25175	177 - 244	9.6e-06	WDR5 beta-propeller domain
Beta-prop_EML_2	PF23414	177 - 244	9.8e-07	Echinoderm microtubule-associated protein second beta-propeller

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

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Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0007211)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G21060
fragaria_vesca	FvH4_2g10510 FvH4_2g10510 FvH4_2g10510 FvH4_2g10510
malus_domestica	MD02G1259400.v1.1 MD07G1061200.v1.1
prunus_persica	Prupe.5G058900_v2.0.a1 Prupe.5G058900_v2.0.a1
pyrus_communis	pycom02g22080
rosa_chinensis	RchiOBHm_Chr6g0268021
rosa_laevigata	RLG00000013939
rosa_multiflora	Rmu_sc0001839.1_g000031 Rmu_sc0003304.1_g000001 Rmu_sc0003304.1_g000039 Rmu_sc0004529.1_g000038
rosa_roxburghii	Rroxscaffold_7G00200120 Rroxscaffold_7G00200140
rosa_rugosa	Rorug06G0038800
rosa_samantha	Rh6AG158100 Rh6BG162100 Rh6CG156300 Rh6DG147700
rosa_wichuraiana	Rw0G021300 Rw6G013760

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	606
AccII	CGCG	2 cut(s)	243, 490
AccIII	TCCGGA	2 cut(s)	457, 1336
AciI	CCGC	2 cut(s)	490, 1457
AclWI	GGATC	6 cut(s)	564, 832, 1392, 1405, 1463, 1476
AcsI	RAATTY	3 cut(s)	700, 987, 1175
AfaI	GTAC	3 cut(s)	106, 474, 1157
AfiI	CCNNNNNNNGG	2 cut(s)	29, 641
AhlI	ACTAGT	1 cut(s)	212
AjiI	CACGTC	1 cut(s)	1433
AjnI	CCWGG	4 cut(s)	161, 352, 378, 1471
AluBI	AGCT	7 cut(s)	181, 260, 341, 1138, 1268, 1300, 1531
AluI	AGCT	7 cut(s)	181, 260, 341, 1138, 1268, 1300, 1531
Alw21I	GWGCWC	3 cut(s)	128, 183, 1140
Alw26I	GTCTC	2 cut(s)	152, 1057
AlwI	GGATC	6 cut(s)	564, 832, 1392, 1405, 1463, 1476
AlwNI	CAGNNNCTG	1 cut(s)	691
Ama87I	CYCGRG	1 cut(s)	1139
Aor13HI	TCCGGA	2 cut(s)	457, 1336
AoxI	GGCC	2 cut(s)	998, 1203
ApeKI	GCWGC	5 cut(s)	116, 203, 257, 533, 1528
ApoI	RAATTY	3 cut(s)	700, 987, 1175
Asp700I	GAANNNNTTC	1 cut(s)	591
AspS9I	GGNCC	1 cut(s)	998
AsuHPI	GGTGA	3 cut(s)	308, 914, 920
AvaI	CYCGRG	1 cut(s)	1139
BamHI	GGATCC	2 cut(s)	1397, 1468
BanII	GRGCYC	2 cut(s)	183, 1140
Bbv12I	GWGCWC	3 cut(s)	128, 183, 1140
BbvI	GCAGC	4 cut(s)	103, 190, 269, 520
BccI	CCATC	8 cut(s)	125, 376, 493, 500, 746, 782, 872, 899
BceAI	ACGGC	1 cut(s)	84
BcgI	CGANNNNNNTGC	2 cut(s)	558, 592
BciT130I	CCWGG	4 cut(s)	163, 354, 380, 1473
BcoDI	GTCTC	2 cut(s)	152, 1057
BcuI	ACTAGT	1 cut(s)	212
BfaI	CTAG	2 cut(s)	213, 794
BfmI	CTRYAG	1 cut(s)	255
BisI	GCNGC	5 cut(s)	117, 204, 258, 534, 1529
BlsI	GCNGC	5 cut(s)	118, 205, 259, 535, 1530
BmcAI	AGTACT	1 cut(s)	474
Bme1390I	CCNGG	4 cut(s)	163, 354, 380, 1473
BmeT110I	CYCGRG	1 cut(s)	1139
BmgBI	CACGTC	1 cut(s)	1433
BmgT120I	GGNCC	1 cut(s)	998
BmiI	GGNNCC	5 cut(s)	358, 633, 649, 1399, 1470
BmrFI	CCNGG	4 cut(s)	163, 354, 380, 1473
BmrI	ACTGGG	1 cut(s)	464
BmsI	GCATC	6 cut(s)	790, 864, 985, 1039, 1321, 1457
BmuI	ACTGGG	1 cut(s)	464
BpmI	CTGGAG	3 cut(s)	663, 1129, 1494
Bpu10I	CCTNAGC	1 cut(s)	174
Bsa29I	ATCGAT	1 cut(s)	1017
BsaI	GGTCTC	1 cut(s)	152
BsaJI	CCNNGG	3 cut(s)	100, 162, 627
BsaWI	WCCGGW	2 cut(s)	457, 1336
BsaXI	ACNNNNNCTCC	2 cut(s)	636, 666
Bsc4I	CCNNNNNNNGG	2 cut(s)	29, 641
Bse1I	ACTGG	3 cut(s)	446, 470, 1112
Bse3DI	GCAATG	3 cut(s)	84, 168, 1443
BseAI	TCCGGA	2 cut(s)	457, 1336
BseBI	CCWGG	4 cut(s)	163, 354, 380, 1473
BseCI	ATCGAT	1 cut(s)	1017
BseDI	CCNNGG	3 cut(s)	100, 162, 627
BseGI	GGATG	3 cut(s)	349, 1030, 1039
BseLI	CCNNNNNNNGG	2 cut(s)	29, 641
BseMI	GCAATG	3 cut(s)	84, 168, 1443
BseMII	CTCAG	2 cut(s)	795, 1092
BseNI	ACTGG	3 cut(s)	446, 470, 1112
BseRI	GAGGAG	2 cut(s)	68, 1166
BseXI	GCAGC	4 cut(s)	103, 190, 269, 520
BseYI	CCCAGC	2 cut(s)	119, 971
Bsh1236I	CGCG	2 cut(s)	243, 490
BshFI	GGCC	2 cut(s)	1000, 1205
BshVI	ATCGAT	1 cut(s)	1017
BsiHKAI	GWGCWC	3 cut(s)	128, 183, 1140
BsiHKCI	CYCGRG	1 cut(s)	1139
BsiSI	CCGG	2 cut(s)	458, 1337
BslFI	GGGAC	2 cut(s)	296, 977
BslI	CCNNNNNNNGG	2 cut(s)	29, 641
BsmAI	GTCTC	2 cut(s)	152, 1057
BsmFI	GGGAC	2 cut(s)	296, 977
BsnI	GGCC	2 cut(s)	1000, 1205
Bso31I	GGTCTC	1 cut(s)	152
BsoBI	CYCGRG	1 cut(s)	1139
Bsp1286I	GDGCHC	3 cut(s)	128, 183, 1140
Bsp13I	TCCGGA	2 cut(s)	457, 1336
Bsp143I	GATC	6 cut(s)	556, 824, 1014, 1180, 1397, 1468
Bsp19I	CCATGG	1 cut(s)	627
BspACI	CCGC	2 cut(s)	490, 1457
BspANI	GGCC	2 cut(s)	1000, 1205
BspCNI	CTCAG	2 cut(s)	796, 1093
BspDI	ATCGAT	1 cut(s)	1017
BspEI	TCCGGA	2 cut(s)	457, 1336
BspFNI	CGCG	2 cut(s)	243, 490
BspLI	GGNNCC	5 cut(s)	358, 633, 649, 1399, 1470
BspMAI	CTGCAG	1 cut(s)	259
BspPI	GGATC	6 cut(s)	564, 832, 1392, 1405, 1463, 1476
BspQI	GCTCTTC	1 cut(s)	1290
BspTNI	GGTCTC	1 cut(s)	152
BsrDI	GCAATG	3 cut(s)	84, 168, 1443
BsrI	ACTGG	3 cut(s)	446, 470, 1112
BssECI	CCNNGG	3 cut(s)	100, 162, 627
BssMI	GATC	6 cut(s)	556, 824, 1014, 1180, 1397, 1468
BssT1I	CCWWGG	1 cut(s)	627
Bst2UI	CCWGG	4 cut(s)	163, 354, 380, 1473
Bst4CI	ACNGT	3 cut(s)	197, 613, 1252
Bst6I	CTCTTC	4 cut(s)	114, 1290, 1407, 1458
BstC8I	GCNNGC	5 cut(s)	385, 538, 624, 934, 1314
BstDEI	CTNAG	4 cut(s)	174, 804, 1101, 1357
BstDSI	CCRYGG	2 cut(s)	100, 627
BstF5I	GGATG	3 cut(s)	349, 1030, 1039
BstFNI	CGCG	2 cut(s)	243, 490
BstKTI	GATC	6 cut(s)	559, 827, 1017, 1183, 1400, 1471
BstMAI	GTCTC	2 cut(s)	152, 1057
BstMBI	GATC	6 cut(s)	556, 824, 1014, 1180, 1397, 1468
BstMWI	GCNNNNNNNGC	1 cut(s)	384
BstNI	CCWGG	4 cut(s)	163, 354, 380, 1473
BstSCI	CCNGG	4 cut(s)	161, 352, 378, 1471
BstSFI	CTRYAG	1 cut(s)	255
BstUI	CGCG	2 cut(s)	243, 490
BstV1I	GCAGC	4 cut(s)	103, 190, 269, 520
BstX2I	RGATCY	3 cut(s)	556, 1397, 1468
BstYI	RGATCY	3 cut(s)	556, 1397, 1468
Bsu15I	ATCGAT	1 cut(s)	1017
BsuRI	GGCC	2 cut(s)	1000, 1205
BsuTUI	ATCGAT	1 cut(s)	1017
BtgI	CCRYGG	2 cut(s)	100, 627
BtgZI	GCGATG	2 cut(s)	224, 471
BtrI	CACGTC	1 cut(s)	1433
BtsCI	GGATG	3 cut(s)	349, 1030, 1039
BtsI	GCAGTG	1 cut(s)	23
BtsIMutI	CAGTG	5 cut(s)	23, 904, 1098, 1257, 1279
Cac8I	GCNNGC	5 cut(s)	385, 538, 624, 934, 1314
CaiI	CAGNNNCTG	1 cut(s)	691
Cfr13I	GGNCC	1 cut(s)	998
ClaI	ATCGAT	1 cut(s)	1017
Csp6I	GTAC	3 cut(s)	105, 473, 1156
CspCI	CAANNNNNGTGG	4 cut(s)	82, 117, 1359, 1394
CviAII	CATG	4 cut(s)	68, 406, 628, 1445
CviQI	GTAC	3 cut(s)	105, 473, 1156
DdeI	CTNAG	4 cut(s)	174, 804, 1101, 1357
DpnI	GATC	6 cut(s)	558, 826, 1016, 1182, 1399, 1470
DpnII	GATC	6 cut(s)	556, 824, 1014, 1180, 1397, 1468
Eam1104I	CTCTTC	4 cut(s)	114, 1290, 1407, 1458
EarI	CTCTTC	4 cut(s)	114, 1290, 1407, 1458
Ecl136II	GAGCTC	2 cut(s)	181, 1138
Eco130I	CCWWGG	1 cut(s)	627
Eco24I	GRGCYC	2 cut(s)	183, 1140
Eco31I	GGTCTC	1 cut(s)	152
Eco32I	GATATC	1 cut(s)	1243
Eco53kI	GAGCTC	2 cut(s)	181, 1138
Eco88I	CYCGRG	1 cut(s)	1139
EcoICRI	GAGCTC	2 cut(s)	181, 1138
EcoRII	CCWGG	4 cut(s)	161, 352, 378, 1471
EcoRV	GATATC	1 cut(s)	1243
EcoT14I	CCWWGG	1 cut(s)	627
EcoT22I	ATGCAT	3 cut(s)	86, 879, 1450
EcoT38I	GRGCYC	2 cut(s)	183, 1140
ErhI	CCWWGG	1 cut(s)	627
FaeI	CATG	4 cut(s)	71, 409, 631, 1448
FalI	AAGNNNNNCTT	2 cut(s)	995, 1027
FaqI	GGGAC	2 cut(s)	296, 977
FatI	CATG	4 cut(s)	67, 405, 627, 1444
FauNDI	CATATG	1 cut(s)	959
Fnu4HI	GCNGC	5 cut(s)	117, 204, 258, 534, 1529
FokI	GGATG	3 cut(s)	336, 1017, 1026
FriOI	GRGCYC	2 cut(s)	183, 1140
Fsp4HI	GCNGC	5 cut(s)	117, 204, 258, 534, 1529
FspBI	CTAG	2 cut(s)	213, 794
GluI	GCNGC	5 cut(s)	117, 204, 258, 534, 1529
GsaI	CCCAGC	2 cut(s)	123, 975
GsuI	CTGGAG	3 cut(s)	663, 1129, 1494
HaeIII	GGCC	2 cut(s)	1000, 1205
HapII	CCGG	2 cut(s)	458, 1337
Hin1II	CATG	4 cut(s)	71, 409, 631, 1448
HincII	GTYRAC	2 cut(s)	273, 415
HindII	GTYRAC	2 cut(s)	273, 415
HinfI	GANTC	5 cut(s)	461, 1211, 1262, 1277, 1441
HpaII	CCGG	2 cut(s)	458, 1337
HphI	GGTGA	3 cut(s)	308, 914, 920
Hpy166II	GTNNAC	3 cut(s)	273, 415, 1255
Hpy188I	TCNGA	6 cut(s)	130, 505, 1185, 1282, 1411, 1510
Hpy188III	TCNNGA	9 cut(s)	458, 642, 785, 857, 992, 1124, 1246, 1337, 1395
Hpy8I	GTNNAC	3 cut(s)	273, 415, 1255
HpyAV	CCTTC	4 cut(s)	100, 746, 989, 1515
HpyCH4III	ACNGT	3 cut(s)	197, 613, 1252
HpyCH4IV	ACGT	2 cut(s)	285, 1432
HpyF10VI	GCNNNNNNNGC	1 cut(s)	384
HpyF3I	CTNAG	4 cut(s)	174, 804, 1101, 1357
HpySE526I	ACGT	2 cut(s)	285, 1432
Hsp92II	CATG	4 cut(s)	71, 409, 631, 1448
Kpn2I	TCCGGA	2 cut(s)	457, 1336
Kzo9I	GATC	6 cut(s)	556, 824, 1014, 1180, 1397, 1468
LguI	GCTCTTC	1 cut(s)	1290
LmnI	GCTCC	8 cut(s)	178, 186, 406, 1126, 1135, 1339, 1339, 1475
Lsp1109I	GCAGC	4 cut(s)	103, 190, 269, 520
LweI	GCATC	6 cut(s)	790, 864, 985, 1039, 1321, 1457
MaeI	CTAG	2 cut(s)	213, 794
MaeII	ACGT	2 cut(s)	285, 1432
MaeIII	GTNAC	3 cut(s)	345, 1246, 1287
MalI	GATC	6 cut(s)	558, 826, 1016, 1182, 1399, 1470
MboI	GATC	6 cut(s)	556, 824, 1014, 1180, 1397, 1468
MboII	GAAGA	7 cut(s)	101, 313, 1181, 1238, 1307, 1424, 1475
MflI	RGATCY	3 cut(s)	556, 1397, 1468
MhlI	GDGCHC	3 cut(s)	128, 183, 1140
MluCI	AATT	7 cut(s)	136, 700, 918, 987, 1175, 1317, 1390
MlyI	GAGTC	1 cut(s)	455
MmeI	TCCRAC	2 cut(s)	802, 1500
Mph1103I	ATGCAT	3 cut(s)	86, 879, 1450
MroI	TCCGGA	2 cut(s)	457, 1336
MroXI	GAANNNNTTC	1 cut(s)	591
MseI	TTAA	6 cut(s)	543, 657, 776, 836, 1131, 1221
MslI	CAYNNNNRTG	1 cut(s)	872
MspA1I	CMGCKG	1 cut(s)	260
MspI	CCGG	2 cut(s)	458, 1337
MspR9I	CCNGG	4 cut(s)	163, 354, 380, 1473
MvaI	CCWGG	4 cut(s)	163, 354, 380, 1473
MvnI	CGCG	2 cut(s)	243, 490
MwoI	GCNNNNNNNGC	1 cut(s)	384
NcoI	CCATGG	1 cut(s)	627
NdeI	CATATG	1 cut(s)	959
NdeII	GATC	6 cut(s)	556, 824, 1014, 1180, 1397, 1468
NlaIII	CATG	4 cut(s)	71, 409, 631, 1448
NlaIV	GGNNCC	5 cut(s)	358, 633, 649, 1399, 1470
NmuCI	GTSAC	2 cut(s)	1246, 1287
NsiI	ATGCAT	3 cut(s)	86, 879, 1450
PaeR7I	CTCGAG	1 cut(s)	1139
PciSI	GCTCTTC	1 cut(s)	1290
PdmI	GAANNNNTTC	1 cut(s)	591
PfeI	GAWTC	4 cut(s)	1211, 1262, 1277, 1441
PfoI	TCCNGGA	1 cut(s)	1471
PkrI	GCNGC	5 cut(s)	118, 205, 259, 535, 1530
PleI	GAGTC	1 cut(s)	455
PpsI	GAGTC	1 cut(s)	455
PsiI	TTATAA	1 cut(s)	606
Psp124BI	GAGCTC	2 cut(s)	183, 1140
Psp6I	CCWGG	4 cut(s)	161, 352, 378, 1471
PspFI	CCCAGC	2 cut(s)	119, 971
PspGI	CCWGG	4 cut(s)	161, 352, 378, 1471
PspN4I	GGNNCC	5 cut(s)	358, 633, 649, 1399, 1470
PspPI	GGNCC	1 cut(s)	998
PspXI	VCTCGAGB	1 cut(s)	1139
PstI	CTGCAG	1 cut(s)	259
PstNI	CAGNNNCTG	1 cut(s)	691
PsuI	RGATCY	3 cut(s)	556, 1397, 1468
PvuII	CAGCTG	1 cut(s)	260
RsaI	GTAC	3 cut(s)	106, 474, 1157
RsaNI	GTAC	3 cut(s)	105, 473, 1156
RseI	CAYNNNNRTG	1 cut(s)	872
SacI	GAGCTC	2 cut(s)	183, 1140
SapI	GCTCTTC	1 cut(s)	1290
SaqAI	TTAA	6 cut(s)	543, 657, 776, 836, 1131, 1221
SatI	GCNGC	5 cut(s)	117, 204, 258, 534, 1529
Sau3AI	GATC	6 cut(s)	556, 824, 1014, 1180, 1397, 1468
Sau96I	GGNCC	1 cut(s)	998
ScaI	AGTACT	1 cut(s)	474
SchI	GAGTC	1 cut(s)	455
ScrFI	CCNGG	4 cut(s)	163, 354, 380, 1473
SduI	GDGCHC	3 cut(s)	128, 183, 1140
SfaNI	GCATC	6 cut(s)	790, 864, 985, 1039, 1321, 1457
SfcI	CTRYAG	1 cut(s)	255
Sfr274I	CTCGAG	1 cut(s)	1139
SlaI	CTCGAG	1 cut(s)	1139
SmiMI	CAYNNNNRTG	1 cut(s)	872
SmlI	CTYRAG	1 cut(s)	1139
SmoI	CTYRAG	1 cut(s)	1139
SpeI	ACTAGT	1 cut(s)	212
Sse9I	AATT	7 cut(s)	136, 700, 918, 987, 1175, 1317, 1390
SsiI	CCGC	2 cut(s)	490, 1457
SspMI	CTAG	2 cut(s)	213, 794
SstI	GAGCTC	2 cut(s)	183, 1140
StyD4I	CCNGG	4 cut(s)	161, 352, 378, 1471
StyI	CCWWGG	1 cut(s)	627
TaaI	ACNGT	3 cut(s)	197, 613, 1252
TaiI	ACGT	2 cut(s)	288, 1435
TaqI	TCGA	4 cut(s)	18, 578, 1017, 1140
TasI	AATT	7 cut(s)	136, 700, 918, 987, 1175, 1317, 1390
TatI	WGTACW	2 cut(s)	472, 1155
TfiI	GAWTC	4 cut(s)	1211, 1262, 1277, 1441
Tru1I	TTAA	6 cut(s)	543, 657, 776, 836, 1131, 1221
Tru9I	TTAA	6 cut(s)	543, 657, 776, 836, 1131, 1221
TscAI	CASTG	5 cut(s)	30, 904, 1105, 1257, 1279
TseFI	GTSAC	2 cut(s)	1246, 1287
TseI	GCWGC	5 cut(s)	116, 203, 257, 533, 1528
Tsp45I	GTSAC	2 cut(s)	1246, 1287
TspDTI	ATGAA	8 cut(s)	17, 92, 1028, 1188, 1239, 1248, 1356, 1454
TspGWI	ACGGA	3 cut(s)	173, 640, 836
TspRI	CASTG	5 cut(s)	30, 904, 1105, 1257, 1279
XapI	RAATTY	3 cut(s)	700, 987, 1175
XhoI	CTCGAG	1 cut(s)	1139
XmnI	GAANNNNTTC	1 cut(s)	591
XspI	CTAG	2 cut(s)	213, 794
ZrmI	AGTACT	1 cut(s)	474
Zsp2I	ATGCAT	3 cut(s)	86, 879, 1450

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.