MD07G1066500.v1.1

Alkaline neutral invertase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0016415
Physical Location & Seq
Reverse (-)
6278995 .. 6279785
791 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1066500.v1.1.491

Sequence Viewer

Length: 333 bp
ATGCAGCTCACTGTCGCTTGCATAAAGATGGGTAGACCTGAAATTGCTGCAAAAGCTGTTGAGATTGCCGAGAAACGGGTTTCGCGAGACAATTGGCCAGAATACTATGATACCAAAAGAGGTAGGTTCATTGGAAAACAAGCACACCTGTTCCGGGCCTGGTCAATTGCAGGATACCCTGTCGTAAAGCTCCTGCTCGCTGATCCTGGTAAAGCCAAGATTTTGATAACCGAAGAGGATTCTGAACTCGTGAATGCCTTCTCCTGCATGTTTAGTGCTAACCCAAGAAGAAAGCGAGGTCGAAAGAATTTGAAGCAGACCTACATTGTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009536 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_100

Protein Analysis

111

Amino Acids

12.57

Weight (kDa)

9.95

Isoelectric Point (pI)

55.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_100 PF12899 2 - 69 8.2e-22 Alkaline and neutral invertase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 34
AccII CGCG 1 cut(s) 85
AclWI GGATC 1 cut(s) 197
AcoI YGGCCR 1 cut(s) 95
AcsI RAATTY 1 cut(s) 307
AfiI CCNNNNNNNGG 2 cut(s) 75, 154
AgsI TTSAA 1 cut(s) 313
AjnI CCWGG 2 cut(s) 158, 205
AluBI AGCT 3 cut(s) 7, 56, 190
AluI AGCT 3 cut(s) 7, 56, 190
Alw26I GTCTC 1 cut(s) 81
AlwI GGATC 1 cut(s) 197
AoxI GGCC 2 cut(s) 95, 156
ApeKI GCWGC 2 cut(s) 4, 47
ApoI RAATTY 1 cut(s) 307
Asp700I GAANNNNTTC 1 cut(s) 257
AspS9I GGNCC 1 cut(s) 156
AsuC2I CCSGG 1 cut(s) 155
BalI TGGCCA 1 cut(s) 97
BarI GAAGNNNNNNTAC 1 cut(s) 305
BauI CACGAG 1 cut(s) 248
BbvI GCAGC 2 cut(s) 16, 34
BccI CCATC 1 cut(s) 22
BciT130I CCWGG 2 cut(s) 160, 207
BciVI GTATCC 1 cut(s) 167
BcnI CCSGG 1 cut(s) 155
BcoDI GTCTC 1 cut(s) 81
BfuI GTATCC 1 cut(s) 167
BisI GCNGC 2 cut(s) 5, 48
BlsI GCNGC 2 cut(s) 6, 49
Bme1390I CCNGG 3 cut(s) 155, 160, 207
BmgT120I GGNCC 1 cut(s) 156
BmrFI CCNGG 3 cut(s) 155, 160, 207
BpuMI CCSGG 1 cut(s) 155
Bsc4I CCNNNNNNNGG 2 cut(s) 75, 154
BseBI CCWGG 2 cut(s) 160, 207
BseLI CCNNNNNNNGG 2 cut(s) 75, 154
BseXI GCAGC 2 cut(s) 16, 34
Bsh1236I CGCG 1 cut(s) 85
BshFI GGCC 2 cut(s) 97, 158
BsiSI CCGG 1 cut(s) 154
BslI CCNNNNNNNGG 2 cut(s) 75, 154
BsmAI GTCTC 1 cut(s) 81
BsmI GAATGC 1 cut(s) 259
BsnI GGCC 2 cut(s) 97, 158
Bsp143I GATC 1 cut(s) 202
Bsp68I TCGCGA 1 cut(s) 85
BspANI GGCC 2 cut(s) 97, 158
BspFNI CGCG 1 cut(s) 85
BspPI GGATC 1 cut(s) 197
BssMI GATC 1 cut(s) 202
BssSI CACGAG 1 cut(s) 248
Bst2BI CACGAG 1 cut(s) 248
Bst2UI CCWGG 2 cut(s) 160, 207
Bst4CI ACNGT 1 cut(s) 13
Bst6I CTCTTC 1 cut(s) 228
BstC8I GCNNGC 2 cut(s) 19, 198
BstFNI CGCG 1 cut(s) 85
BstKTI GATC 1 cut(s) 205
BstMAI GTCTC 1 cut(s) 81
BstMBI GATC 1 cut(s) 202
BstMWI GCNNNNNNNGC 1 cut(s) 53
BstNI CCWGG 2 cut(s) 160, 207
BstNSI RCATGY 1 cut(s) 271
BstSCI CCNGG 3 cut(s) 153, 158, 205
BstUI CGCG 1 cut(s) 85
BstV1I GCAGC 2 cut(s) 16, 34
BsuI GTATCC 1 cut(s) 167
BsuRI GGCC 2 cut(s) 97, 158
BtsIMutI CAGTG 1 cut(s) 9
BtuMI TCGCGA 1 cut(s) 85
Cac8I GCNNGC 2 cut(s) 19, 198
Cfr13I GGNCC 1 cut(s) 156
CviAII CATG 1 cut(s) 268
CviJI RGCY 6 cut(s) 7, 56, 97, 158, 190, 215
CviKI_1 RGCY 6 cut(s) 7, 56, 97, 158, 190, 215
DpnI GATC 1 cut(s) 204
DpnII GATC 1 cut(s) 202
EaeI YGGCCR 1 cut(s) 95
Eam1104I CTCTTC 1 cut(s) 228
EarI CTCTTC 1 cut(s) 228
EcoRII CCWGG 2 cut(s) 158, 205
FaeI CATG 1 cut(s) 271
FaiI YATR 4 cut(s) 23, 108, 269, 331
FatI CATG 1 cut(s) 267
FblI GTMKAC 1 cut(s) 34
Fnu4HI GCNGC 2 cut(s) 5, 48
Fsp4HI GCNGC 2 cut(s) 5, 48
GluI GCNGC 2 cut(s) 5, 48
HaeIII GGCC 2 cut(s) 97, 158
HapII CCGG 1 cut(s) 154
Hin1II CATG 1 cut(s) 271
HinfI GANTC 1 cut(s) 239
HpaII CCGG 1 cut(s) 154
Hpy166II GTNNAC 1 cut(s) 35
Hpy188I TCNGA 1 cut(s) 244
Hpy188III TCNNGA 2 cut(s) 84, 250
Hpy8I GTNNAC 1 cut(s) 35
HpyAV CCTTC 1 cut(s) 268
HpyCH4III ACNGT 1 cut(s) 13
HpyCH4V TGCA 5 cut(s) 4, 21, 50, 170, 267
HpyF10VI GCNNNNNNNGC 1 cut(s) 53
Hsp92II CATG 1 cut(s) 271
Kzo9I GATC 1 cut(s) 202
LmnI GCTCC 1 cut(s) 195
Lsp1109I GCAGC 2 cut(s) 16, 34
MalI GATC 1 cut(s) 204
MboI GATC 1 cut(s) 202
MboII GAAGA 2 cut(s) 245, 300
MfeI CAATTG 2 cut(s) 91, 165
MlsI TGGCCA 1 cut(s) 97
MluCI AATT 4 cut(s) 42, 91, 165, 307
MluNI TGGCCA 1 cut(s) 97
MnlI CCTC 3 cut(s) 113, 229, 290
Mox20I TGGCCA 1 cut(s) 97
MroXI GAANNNNTTC 1 cut(s) 257
MscI TGGCCA 1 cut(s) 97
MslI CAYNNNNRTG 1 cut(s) 26
Msp20I TGGCCA 1 cut(s) 97
MspI CCGG 1 cut(s) 154
MspR9I CCNGG 3 cut(s) 155, 160, 207
MunI CAATTG 2 cut(s) 91, 165
Mva1269I GAATGC 1 cut(s) 259
MvaI CCWGG 2 cut(s) 160, 207
MvnI CGCG 1 cut(s) 85
MwoI GCNNNNNNNGC 1 cut(s) 53
NciI CCSGG 1 cut(s) 155
NdeII GATC 1 cut(s) 202
NlaIII CATG 1 cut(s) 271
NmeAIII GCCGAG 1 cut(s) 94
NruI TCGCGA 1 cut(s) 85
NspI RCATGY 1 cut(s) 271
PcsI WCGNNNNNNNCGW 1 cut(s) 82
PctI GAATGC 1 cut(s) 259
PdmI GAANNNNTTC 1 cut(s) 257
PfeI GAWTC 1 cut(s) 239
PkrI GCNGC 2 cut(s) 6, 49
Psp6I CCWGG 2 cut(s) 158, 205
PspGI CCWGG 2 cut(s) 158, 205
PspPI GGNCC 1 cut(s) 156
RruI TCGCGA 1 cut(s) 85
RseI CAYNNNNRTG 1 cut(s) 26
SatI GCNGC 2 cut(s) 5, 48
Sau3AI GATC 1 cut(s) 202
Sau96I GGNCC 1 cut(s) 156
ScrFI CCNGG 3 cut(s) 155, 160, 207
SetI ASST 9 cut(s) 9, 40, 58, 124, 128, 150, 192, 301, 323
SmiMI CAYNNNNRTG 1 cut(s) 26
Sse9I AATT 4 cut(s) 42, 91, 165, 307
StyD4I CCNGG 3 cut(s) 153, 158, 205
TaaI ACNGT 1 cut(s) 13
TaqI TCGA 1 cut(s) 301
TasI AATT 4 cut(s) 42, 91, 165, 307
TfiI GAWTC 1 cut(s) 239
TscAI CASTG 1 cut(s) 16
TseI GCWGC 2 cut(s) 4, 47
TspDTI ATGAA 1 cut(s) 118
TspRI CASTG 1 cut(s) 16
XapI RAATTY 1 cut(s) 307
XceI RCATGY 1 cut(s) 271
XmiI GTMKAC 1 cut(s) 34
XmnI GAANNNNTTC 1 cut(s) 257
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.