Prupe.6G291200_v2.0.a1

Alkaline neutral invertase CINV2-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp06
OG0016415
Physical Location & Seq
Reverse (-)
26864548 .. 26869512
4965 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.6G291200.1

Sequence Viewer

Length: 537 bp
ATGGAAGGCGGAGATGGCGGTGCAACGACGGAAAATTCCGGGTTGAACCAATCGATACTGCCGACGATTCTTGAGTTTTCCGGTTGGGAAACTGGTGGTTCGGGTTCGGGACTTCGAGCCAGAGCTAATGGGACCGGTGCTGTTGATCGAGGTGGCCGAAAATGGATTTTACTTGTAAAGAAGATTTCCGAACAACAACCCAGAGAAGTTTCATGCAGTTATCACAATGGTGGCTCTTGGCCAACACTGCTGTGGTTGTGTACTGCAGCATGTATCAAGACGGGGAAGCCTCAGACTGTTAAGAGAGCAATTGAACAGGTGGAGCAGAGGCTCTCAAAAGACCGGTGGCCAGAATACTATGACGGCAAGGCAGGGAGATATATCAGGAAGCAAGCGAGGAAGTACCAGACCTGGAGCATCTCAGGATATCTGGTAGCTAAACTGATGATCGAGAACCCAGTCAATCTTTCACTAATCTCTCTTGAAGAGGACAAGAAGATAGCCAAGCCAAGGCTCACCCGCTCCACTTCCTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_100

Protein Analysis

179

Amino Acids

19.74

Weight (kDa)

9.68

Isoelectric Point (pI)

46.77

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 522
AciI CCGC 3 cut(s) 9, 18, 520
AcoI YGGCCR 3 cut(s) 154, 239, 347
AcsI RAATTY 1 cut(s) 34
AfaI GTAC 2 cut(s) 262, 404
AfiI CCNNNNNNNGG 1 cut(s) 510
AgeI ACCGGT 2 cut(s) 134, 342
AgsI TTSAA 3 cut(s) 46, 314, 485
AjnI CCWGG 1 cut(s) 410
AleI CACNNNNGTG 2 cut(s) 228, 250
AluBI AGCT 2 cut(s) 125, 437
AluI AGCT 2 cut(s) 125, 437
AoxI GGCC 3 cut(s) 154, 239, 347
ApeKI GCWGC 1 cut(s) 266
ApoI RAATTY 1 cut(s) 34
AsiGI ACCGGT 2 cut(s) 134, 342
AspS9I GGNCC 1 cut(s) 132
AsuC2I CCSGG 1 cut(s) 40
AsuHPI GGTGA 1 cut(s) 508
AvaII GGWCC 1 cut(s) 132
BalI TGGCCA 2 cut(s) 241, 349
BbvI GCAGC 1 cut(s) 278
BccI CCATC 1 cut(s) 8
BceAI ACGGC 1 cut(s) 379
BciT130I CCWGG 1 cut(s) 412
BcnI CCSGG 1 cut(s) 40
BfmI CTRYAG 1 cut(s) 264
BisI GCNGC 1 cut(s) 267
BlsI GCNGC 1 cut(s) 268
Bme1390I CCNGG 2 cut(s) 40, 412
Bme18I GGWCC 1 cut(s) 132
BmgT120I GGNCC 1 cut(s) 132
BmiI GGNNCC 1 cut(s) 133
BmrFI CCNGG 2 cut(s) 40, 412
BmrI ACTGGG 1 cut(s) 452
BmsI GCATC 1 cut(s) 426
BmuI ACTGGG 1 cut(s) 452
BpmI CTGGAG 1 cut(s) 433
BpuEI CTTGAG 1 cut(s) 92
BpuMI CCSGG 1 cut(s) 40
Bsa29I ATCGAT 1 cut(s) 53
BsaJI CCNNGG 1 cut(s) 509
BsaWI WCCGGW 3 cut(s) 80, 134, 342
Bsc4I CCNNNNNNNGG 1 cut(s) 510
Bse118I RCCGGY 2 cut(s) 134, 342
Bse1I ACTGG 2 cut(s) 97, 458
BseBI CCWGG 1 cut(s) 412
BseCI ATCGAT 1 cut(s) 53
BseDI CCNNGG 1 cut(s) 509
BseLI CCNNNNNNNGG 1 cut(s) 510
BseMII CTCAG 2 cut(s) 305, 435
BseNI ACTGG 2 cut(s) 97, 458
BseXI GCAGC 1 cut(s) 278
BshFI GGCC 3 cut(s) 156, 241, 349
BshTI ACCGGT 2 cut(s) 134, 342
BshVI ATCGAT 1 cut(s) 53
BsiSI CCGG 4 cut(s) 39, 81, 135, 343
BslFI GGGAC 2 cut(s) 123, 145
BslI CCNNNNNNNGG 1 cut(s) 510
BsmFI GGGAC 2 cut(s) 123, 145
BsnI GGCC 3 cut(s) 156, 241, 349
Bsp143I GATC 2 cut(s) 145, 447
BspACI CCGC 3 cut(s) 9, 18, 520
BspANI GGCC 3 cut(s) 156, 241, 349
BspCNI CTCAG 2 cut(s) 304, 434
BspDI ATCGAT 1 cut(s) 53
BspLI GGNNCC 1 cut(s) 133
BspMAI CTGCAG 1 cut(s) 268
BsrBI CCGCTC 1 cut(s) 522
BsrFI RCCGGY 2 cut(s) 134, 342
BsrI ACTGG 2 cut(s) 97, 458
BssAI RCCGGY 2 cut(s) 134, 342
BssECI CCNNGG 1 cut(s) 509
BssMI GATC 2 cut(s) 145, 447
BssT1I CCWWGG 1 cut(s) 509
Bst2UI CCWGG 1 cut(s) 412
Bst4CI ACNGT 1 cut(s) 298
Bst6I CTCTTC 1 cut(s) 480
BstC8I GCNNGC 1 cut(s) 393
BstDEI CTNAG 2 cut(s) 291, 421
BstKTI GATC 2 cut(s) 148, 450
BstMBI GATC 2 cut(s) 145, 447
BstMWI GCNNNNNNNGC 2 cut(s) 15, 247
BstNI CCWGG 1 cut(s) 412
BstNSI RCATGY 1 cut(s) 273
BstSCI CCNGG 2 cut(s) 38, 410
BstSFI CTRYAG 1 cut(s) 264
BstV1I GCAGC 1 cut(s) 278
Bsu15I ATCGAT 1 cut(s) 53
BsuRI GGCC 3 cut(s) 156, 241, 349
BsuTUI ATCGAT 1 cut(s) 53
BtsI GCAGTG 1 cut(s) 245
BtsIMutI CAGTG 1 cut(s) 245
Cac8I GCNNGC 1 cut(s) 393
Cfr10I RCCGGY 2 cut(s) 134, 342
Cfr13I GGNCC 1 cut(s) 132
ClaI ATCGAT 1 cut(s) 53
Csp6I GTAC 2 cut(s) 261, 403
CspAI ACCGGT 2 cut(s) 134, 342
CviAII CATG 2 cut(s) 213, 270
CviQI GTAC 2 cut(s) 261, 403
DdeI CTNAG 2 cut(s) 291, 421
DpnI GATC 2 cut(s) 147, 449
DpnII GATC 2 cut(s) 145, 447
EaeI YGGCCR 3 cut(s) 154, 239, 347
Eam1104I CTCTTC 1 cut(s) 480
EarI CTCTTC 1 cut(s) 480
EciI GGCGGA 1 cut(s) 24
Eco130I CCWWGG 1 cut(s) 509
Eco32I GATATC 1 cut(s) 428
Eco47I GGWCC 1 cut(s) 132
EcoRII CCWGG 1 cut(s) 410
EcoRV GATATC 1 cut(s) 428
EcoT14I CCWWGG 1 cut(s) 509
ErhI CCWWGG 1 cut(s) 509
FaeI CATG 2 cut(s) 216, 273
FaiI YATR 4 cut(s) 214, 271, 360, 381
FaqI GGGAC 2 cut(s) 123, 145
FatI CATG 2 cut(s) 212, 269
FauI CCCGC 1 cut(s) 527
Fnu4HI GCNGC 1 cut(s) 267
Fsp4HI GCNGC 1 cut(s) 267
GluI GCNGC 1 cut(s) 267
GsuI CTGGAG 1 cut(s) 433
HaeIII GGCC 3 cut(s) 156, 241, 349
HapII CCGG 4 cut(s) 39, 81, 135, 343
Hin1II CATG 2 cut(s) 216, 273
HinfI GANTC 1 cut(s) 67
HpaII CCGG 4 cut(s) 39, 81, 135, 343
HphI GGTGA 1 cut(s) 508
Hpy166II GTNNAC 1 cut(s) 261
Hpy188I TCNGA 2 cut(s) 190, 294
Hpy188III TCNNGA 7 cut(s) 71, 108, 277, 385, 423, 451, 482
Hpy8I GTNNAC 1 cut(s) 261
Hpy99I CGWCG 2 cut(s) 31, 67
HpyCH4III ACNGT 1 cut(s) 298
HpyCH4V TGCA 3 cut(s) 23, 216, 266
HpyF10VI GCNNNNNNNGC 2 cut(s) 15, 247
HpyF3I CTNAG 2 cut(s) 291, 421
Hsp92II CATG 2 cut(s) 216, 273
Kzo9I GATC 2 cut(s) 145, 447
LmnI GCTCC 3 cut(s) 322, 414, 527
Lsp1109I GCAGC 1 cut(s) 278
LweI GCATC 1 cut(s) 426
MalI GATC 2 cut(s) 147, 449
MbiI CCGCTC 1 cut(s) 522
MboI GATC 2 cut(s) 145, 447
MboII GAAGA 3 cut(s) 193, 497, 508
MfeI CAATTG 1 cut(s) 309
MlsI TGGCCA 2 cut(s) 241, 349
MluCI AATT 2 cut(s) 34, 309
MluNI TGGCCA 2 cut(s) 241, 349
MnlI CCTC 5 cut(s) 143, 300, 321, 390, 481
Mox20I TGGCCA 2 cut(s) 241, 349
MscI TGGCCA 2 cut(s) 241, 349
MseI TTAA 1 cut(s) 300
MslI CAYNNNNRTG 2 cut(s) 228, 250
Msp20I TGGCCA 2 cut(s) 241, 349
MspI CCGG 4 cut(s) 39, 81, 135, 343
MspR9I CCNGG 2 cut(s) 40, 412
MunI CAATTG 1 cut(s) 309
MvaI CCWGG 1 cut(s) 412
MwoI GCNNNNNNNGC 2 cut(s) 15, 247
NciI CCSGG 1 cut(s) 40
NdeII GATC 2 cut(s) 145, 447
NlaIII CATG 2 cut(s) 216, 273
NlaIV GGNNCC 1 cut(s) 133
NspI RCATGY 1 cut(s) 273
OliI CACNNNNGTG 2 cut(s) 228, 250
PcsI WCGNNNNNNNCGW 2 cut(s) 59, 154
PfeI GAWTC 1 cut(s) 67
PinAI ACCGGT 2 cut(s) 134, 342
PkrI GCNGC 1 cut(s) 268
Psp6I CCWGG 1 cut(s) 410
PspGI CCWGG 1 cut(s) 410
PspN4I GGNNCC 1 cut(s) 133
PspPI GGNCC 1 cut(s) 132
PstI CTGCAG 1 cut(s) 268
RsaI GTAC 2 cut(s) 262, 404
RsaNI GTAC 2 cut(s) 261, 403
RseI CAYNNNNRTG 2 cut(s) 228, 250
SaqAI TTAA 1 cut(s) 300
SatI GCNGC 1 cut(s) 267
Sau3AI GATC 2 cut(s) 145, 447
Sau96I GGNCC 1 cut(s) 132
ScrFI CCNGG 2 cut(s) 40, 412
SetI ASST 5 cut(s) 127, 154, 321, 413, 439
SfaNI GCATC 1 cut(s) 426
SfcI CTRYAG 1 cut(s) 264
SinI GGWCC 1 cut(s) 132
SmiMI CAYNNNNRTG 2 cut(s) 228, 250
SmlI CTYRAG 1 cut(s) 71
SmoI CTYRAG 1 cut(s) 71
Sse9I AATT 2 cut(s) 34, 309
SsiI CCGC 3 cut(s) 9, 18, 520
StyD4I CCNGG 2 cut(s) 38, 410
StyI CCWWGG 1 cut(s) 509
TaaI ACNGT 1 cut(s) 298
TaqI TCGA 4 cut(s) 53, 115, 148, 450
TasI AATT 2 cut(s) 34, 309
TatI WGTACW 1 cut(s) 260
TfiI GAWTC 1 cut(s) 67
Tru1I TTAA 1 cut(s) 300
Tru9I TTAA 1 cut(s) 300
TscAI CASTG 1 cut(s) 252
TseI GCWGC 1 cut(s) 266
TspDTI ATGAA 1 cut(s) 201
TspGWI ACGGA 1 cut(s) 44
TspRI CASTG 1 cut(s) 252
VpaK11BI GGWCC 1 cut(s) 132
XapI RAATTY 1 cut(s) 34
XceI RCATGY 1 cut(s) 273
XcmI CCANNNNNNNNNTGG 1 cut(s) 249
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.