MD07G1110800.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0013711
Physical Location & Seq
Reverse (-)
12762550 .. 12763695
1146 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1110800.v1.1.491

Sequence Viewer

Length: 453 bp
ATGGGAGGAGGAACCATGCCTATCATCCACGTCAGACATTCTTCTTCTTCCTCCTCCTCCTCCTCCAACAATCTTCCAGTTCCATCCAGTAGCAGTGTTCAAGTGGGACCCATCAAACCCTCGTGCACTTGTTCATACTGCTTCTGTGACTCTGGGAGTGAGACCTGCAGAAAACATGAACAAGACAAGAGAGCAAGTCGGACGTCGTTGCCGAGCTCCGTTTTTGGTCCAGTTCCGTCCCGATTCGAAGTCGAAAATGCAATCCTTGCTCTTCTCAGTTTTCTGCAGGGAATAACTAGTTCGTCTCAGTCAGAGTTGCAGTGGCTTCAGCCGATATTAGATTCTTGTGTTACAAGAAAGTTGTTATCTTATGGACACAGAAAAGTTTACGATGCTTTCCAATCGTTGCAAACTGATCCTTCTGTTAAGGTGCTGCCTTGTTTTTGCCACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

151

Amino Acids

16.26

Weight (kDa)

8.46

Isoelectric Point (pI)

86.15

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 206
Acc36I ACCTGC 1 cut(s) 173
AclWI GGATC 1 cut(s) 410
AcuI CTGAAG 1 cut(s) 311
AcyI GRCGYC 1 cut(s) 203
AgsI TTSAA 1 cut(s) 101
AhlI ACTAGT 1 cut(s) 296
AjiI CACGTC 1 cut(s) 31
AluBI AGCT 1 cut(s) 216
AluI AGCT 1 cut(s) 216
Alw21I GWGCWC 2 cut(s) 128, 218
Alw26I GTCTC 2 cut(s) 155, 309
Alw44I GTGCAC 1 cut(s) 124
AlwI GGATC 1 cut(s) 410
ApaLI GTGCAC 1 cut(s) 124
ApeKI GCWGC 1 cut(s) 433
AspS9I GGNCC 2 cut(s) 107, 227
AsuII TTCGAA 1 cut(s) 246
AvaII GGWCC 2 cut(s) 107, 227
BaeGI GKGCMC 1 cut(s) 128
BanII GRGCYC 1 cut(s) 218
BauI CACGAG 1 cut(s) 121
Bbv12I GWGCWC 2 cut(s) 128, 218
BbvI GCAGC 1 cut(s) 420
BccI CCATC 2 cut(s) 91, 119
BcoDI GTCTC 2 cut(s) 155, 309
BcuI ACTAGT 1 cut(s) 296
BfaI CTAG 1 cut(s) 297
BfmI CTRYAG 2 cut(s) 166, 284
BfuAI ACCTGC 1 cut(s) 173
BisI GCNGC 1 cut(s) 434
BlsI GCNGC 1 cut(s) 435
Bme18I GGWCC 2 cut(s) 107, 227
BmgBI CACGTC 1 cut(s) 31
BmgT120I GGNCC 2 cut(s) 107, 227
BmiI GGNNCC 3 cut(s) 13, 108, 109
BmsI GCATC 1 cut(s) 382
Bpu14I TTCGAA 1 cut(s) 246
BsaHI GRCGYC 1 cut(s) 203
BsaI GGTCTC 1 cut(s) 155
Bse1I ACTGG 3 cut(s) 77, 87, 230
BseGI GGATG 2 cut(s) 24, 83
BseMII CTCAG 2 cut(s) 289, 320
BseNI ACTGG 3 cut(s) 77, 87, 230
BseRI GAGGAG 5 cut(s) 21, 43, 46, 49, 52
BseSI GKGCMC 1 cut(s) 128
BseXI GCAGC 1 cut(s) 420
BsiHKAI GWGCWC 2 cut(s) 128, 218
BslFI GGGAC 2 cut(s) 120, 223
BsmAI GTCTC 2 cut(s) 155, 309
BsmBI CGTCTC 1 cut(s) 309
BsmFI GGGAC 2 cut(s) 120, 223
Bso31I GGTCTC 1 cut(s) 155
Bsp119I TTCGAA 1 cut(s) 246
Bsp1286I GDGCHC 2 cut(s) 128, 218
Bsp143I GATC 1 cut(s) 415
BspCNI CTCAG 2 cut(s) 288, 319
BspLI GGNNCC 3 cut(s) 13, 108, 109
BspMAI CTGCAG 2 cut(s) 170, 288
BspMI ACCTGC 1 cut(s) 173
BspPI GGATC 1 cut(s) 410
BspQI GCTCTTC 1 cut(s) 276
BspT104I TTCGAA 1 cut(s) 246
BspTNI GGTCTC 1 cut(s) 155
BsrI ACTGG 3 cut(s) 77, 87, 230
BssMI GATC 1 cut(s) 415
BssNI GRCGYC 1 cut(s) 203
BssSI CACGAG 1 cut(s) 121
Bst2BI CACGAG 1 cut(s) 121
Bst6I CTCTTC 1 cut(s) 276
BstACI GRCGYC 1 cut(s) 203
BstAPI GCANNNNNTGC 1 cut(s) 266
BstBI TTCGAA 1 cut(s) 246
BstDEI CTNAG 2 cut(s) 275, 306
BstF5I GGATG 2 cut(s) 24, 83
BstKTI GATC 1 cut(s) 418
BstMAI GTCTC 2 cut(s) 155, 309
BstMBI GATC 1 cut(s) 415
BstMWI GCNNNNNNNGC 1 cut(s) 266
BstSFI CTRYAG 2 cut(s) 166, 284
BstSLI GKGCMC 1 cut(s) 128
BstV1I GCAGC 1 cut(s) 420
BtrI CACGTC 1 cut(s) 31
BtsCI GGATG 2 cut(s) 24, 83
BtsI GCAGTG 2 cut(s) 100, 326
BtsIMutI CAGTG 2 cut(s) 100, 326
BveI ACCTGC 1 cut(s) 173
Cfr13I GGNCC 2 cut(s) 107, 227
CviAII CATG 2 cut(s) 16, 176
CviJI RGCY 3 cut(s) 216, 325, 331
CviKI_1 RGCY 3 cut(s) 216, 325, 331
DdeI CTNAG 2 cut(s) 275, 306
DpnI GATC 1 cut(s) 417
DpnII GATC 1 cut(s) 415
Eam1104I CTCTTC 1 cut(s) 276
EarI CTCTTC 1 cut(s) 276
Ecl136II GAGCTC 1 cut(s) 216
Eco24I GRGCYC 1 cut(s) 218
Eco31I GGTCTC 1 cut(s) 155
Eco47I GGWCC 2 cut(s) 107, 227
Eco53kI GAGCTC 1 cut(s) 216
Eco57I CTGAAG 1 cut(s) 311
EcoICRI GAGCTC 1 cut(s) 216
EcoO109I RGGNCCY 1 cut(s) 107
EcoT38I GRGCYC 1 cut(s) 218
Esp3I CGTCTC 1 cut(s) 309
FaeI CATG 2 cut(s) 19, 179
FaiI YATR 4 cut(s) 17, 136, 177, 372
FaqI GGGAC 2 cut(s) 120, 223
FatI CATG 2 cut(s) 15, 175
Fnu4HI GCNGC 1 cut(s) 434
FokI GGATG 2 cut(s) 11, 70
FriOI GRGCYC 1 cut(s) 218
Fsp4HI GCNGC 1 cut(s) 434
FspBI CTAG 1 cut(s) 297
GluI GCNGC 1 cut(s) 434
Hin1I GRCGYC 1 cut(s) 203
Hin1II CATG 2 cut(s) 19, 179
HinfI GANTC 3 cut(s) 149, 243, 341
Hpy166II GTNNAC 2 cut(s) 126, 388
Hpy188I TCNGA 3 cut(s) 35, 201, 313
Hpy188III TCNNGA 1 cut(s) 240
Hpy8I GTNNAC 2 cut(s) 126, 388
Hpy99I CGWCG 1 cut(s) 208
HpyAV CCTTC 1 cut(s) 429
HpyCH4IV ACGT 2 cut(s) 30, 203
HpyCH4V TGCA 6 cut(s) 126, 168, 260, 286, 319, 409
HpyF10VI GCNNNNNNNGC 1 cut(s) 266
HpyF3I CTNAG 2 cut(s) 275, 306
HpySE526I ACGT 2 cut(s) 30, 203
Hsp92I GRCGYC 1 cut(s) 203
Hsp92II CATG 2 cut(s) 19, 179
KflI GGGWCCC 1 cut(s) 107
Kzo9I GATC 1 cut(s) 415
LguI GCTCTTC 1 cut(s) 276
LmnI GCTCC 1 cut(s) 221
LpnPI CCDG 6 cut(s) 90, 100, 138, 178, 243, 272
Lsp1109I GCAGC 1 cut(s) 420
LweI GCATC 1 cut(s) 382
MaeI CTAG 1 cut(s) 297
MaeII ACGT 2 cut(s) 30, 203
MaeIII GTNAC 2 cut(s) 146, 349
MalI GATC 1 cut(s) 417
MboI GATC 1 cut(s) 415
MboII GAAGA 5 cut(s) 33, 36, 39, 65, 263
MhlI GDGCHC 2 cut(s) 128, 218
MlyI GAGTC 1 cut(s) 143
MmeI TCCRAC 2 cut(s) 90, 179
MnlI CCTC 6 cut(s) 61, 64, 67, 70, 73, 130
MseI TTAA 1 cut(s) 426
MwoI GCNNNNNNNGC 1 cut(s) 266
NdeII GATC 1 cut(s) 415
NlaIII CATG 2 cut(s) 19, 179
NlaIV GGNNCC 3 cut(s) 13, 108, 109
NmeAIII GCCGAG 1 cut(s) 237
NmuCI GTSAC 1 cut(s) 146
NspV TTCGAA 1 cut(s) 246
PciSI GCTCTTC 1 cut(s) 276
PcsI WCGNNNNNNNCGW 1 cut(s) 209
PfeI GAWTC 2 cut(s) 243, 341
PkrI GCNGC 1 cut(s) 435
PleI GAGTC 1 cut(s) 143
PpsI GAGTC 1 cut(s) 143
PpuMI RGGWCCY 1 cut(s) 107
Psp124BI GAGCTC 1 cut(s) 218
Psp5II RGGWCCY 1 cut(s) 107
PspN4I GGNNCC 3 cut(s) 13, 108, 109
PspPI GGNCC 2 cut(s) 107, 227
PspPPI RGGWCCY 1 cut(s) 107
PstI CTGCAG 2 cut(s) 170, 288
SacI GAGCTC 1 cut(s) 218
SapI GCTCTTC 1 cut(s) 276
SaqAI TTAA 1 cut(s) 426
SatI GCNGC 1 cut(s) 434
Sau3AI GATC 1 cut(s) 415
Sau96I GGNCC 2 cut(s) 107, 227
SchI GAGTC 1 cut(s) 143
SduI GDGCHC 2 cut(s) 128, 218
SetI ASST 5 cut(s) 33, 167, 206, 218, 432
SfaNI GCATC 1 cut(s) 382
SfcI CTRYAG 2 cut(s) 166, 284
SfuI TTCGAA 1 cut(s) 246
SinI GGWCC 2 cut(s) 107, 227
SpeI ACTAGT 1 cut(s) 296
SspMI CTAG 1 cut(s) 297
SstI GAGCTC 1 cut(s) 218
TaiI ACGT 2 cut(s) 33, 206
TaqI TCGA 2 cut(s) 246, 252
TfiI GAWTC 2 cut(s) 243, 341
Tru1I TTAA 1 cut(s) 426
Tru9I TTAA 1 cut(s) 426
TscAI CASTG 2 cut(s) 100, 326
TseFI GTSAC 1 cut(s) 146
TseI GCWGC 1 cut(s) 433
Tsp45I GTSAC 1 cut(s) 146
TspDTI ATGAA 2 cut(s) 123, 192
TspGWI ACGGA 2 cut(s) 208, 225
TspRI CASTG 2 cut(s) 100, 326
VneI GTGCAC 1 cut(s) 124
VpaK11BI GGWCC 2 cut(s) 107, 227
XspI CTAG 1 cut(s) 297
ZraI GACGTC 1 cut(s) 204
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.