MD07G1139100.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0010869
Physical Location & Seq
Reverse (-)
20167868 .. 20170192
2325 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1139100.v1.1.491

Sequence Viewer

Length: 237 bp
ATGAACTCAAAAACCGAAAGGCTGGTGAGGAGGACGACCATGGTGTCCACTGTCACTGCTGCCTATTTCCTCTTGACTGCTGATTACGGCTCCGAGCCCAACGCTCTCGACCCTATTAAGAAAGCTATACAGTCTGCAGAAGGTTCTGTTAAAGACTTCATTTTCAGATCAAAGAGTCCACCTCAGGAAAGCAAAATCGAGAAGCTGGGCGCTACTGATGCAAAGGACCGTCCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

79

Amino Acids

8.6

Weight (kDa)

9.4

Isoelectric Point (pI)

49.58

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 43
AluBI AGCT 2 cut(s) 125, 205
AluI AGCT 2 cut(s) 125, 205
ApeKI GCWGC 1 cut(s) 59
AspLEI GCGC 1 cut(s) 212
AspS9I GGNCC 1 cut(s) 226
AsuHPI GGTGA 1 cut(s) 37
AvaII GGWCC 1 cut(s) 226
AxyI CCTNAGG 1 cut(s) 183
BanII GRGCYC 1 cut(s) 99
BbvI GCAGC 1 cut(s) 46
BceAI ACGGC 1 cut(s) 103
BfmI CTRYAG 1 cut(s) 135
BfoI RGCGCY 1 cut(s) 213
BisI GCNGC 1 cut(s) 60
BlsI GCNGC 1 cut(s) 61
Bme18I GGWCC 1 cut(s) 226
BmgT120I GGNCC 1 cut(s) 226
BmiI GGNNCC 1 cut(s) 91
BmsI GCATC 1 cut(s) 208
BplI GAGNNNNNCTC 2 cut(s) 166, 198
BsaJI CCNNGG 1 cut(s) 39
Bse21I CCTNAGG 1 cut(s) 183
BseDI CCNNGG 1 cut(s) 39
BseMII CTCAG 1 cut(s) 197
BseRI GAGGAG 1 cut(s) 43
BseXI GCAGC 1 cut(s) 46
BseYI CCCAGC 1 cut(s) 205
Bsp1286I GDGCHC 1 cut(s) 99
Bsp143I GATC 1 cut(s) 167
Bsp19I CCATGG 1 cut(s) 39
BspCNI CTCAG 1 cut(s) 196
BspLI GGNNCC 1 cut(s) 91
BspMAI CTGCAG 1 cut(s) 139
BssECI CCNNGG 1 cut(s) 39
BssMI GATC 1 cut(s) 167
BssT1I CCWWGG 1 cut(s) 39
Bst4CI ACNGT 3 cut(s) 52, 132, 230
BstDEI CTNAG 2 cut(s) 183, 234
BstDSI CCRYGG 1 cut(s) 39
BstH2I RGCGCY 1 cut(s) 213
BstHHI GCGC 1 cut(s) 212
BstKTI GATC 1 cut(s) 170
BstMBI GATC 1 cut(s) 167
BstMWI GCNNNNNNNGC 1 cut(s) 218
BstSFI CTRYAG 1 cut(s) 135
BstV1I GCAGC 1 cut(s) 46
Bsu36I CCTNAGG 1 cut(s) 183
BtgI CCRYGG 1 cut(s) 39
BtsI GCAGTG 1 cut(s) 54
BtsIMutI CAGTG 2 cut(s) 48, 54
CfoI GCGC 1 cut(s) 212
Cfr13I GGNCC 1 cut(s) 226
CviAII CATG 1 cut(s) 40
CviJI RGCY 5 cut(s) 22, 90, 97, 125, 205
CviKI_1 RGCY 5 cut(s) 22, 90, 97, 125, 205
DdeI CTNAG 2 cut(s) 183, 234
DpnI GATC 1 cut(s) 169
DpnII GATC 1 cut(s) 167
DrdI GACNNNNNNGTC 1 cut(s) 43
DseDI GACNNNNNNGTC 1 cut(s) 43
Eco130I CCWWGG 1 cut(s) 39
Eco24I GRGCYC 1 cut(s) 99
Eco47I GGWCC 1 cut(s) 226
Eco81I CCTNAGG 1 cut(s) 183
EcoT14I CCWWGG 1 cut(s) 39
EcoT38I GRGCYC 1 cut(s) 99
ErhI CCWWGG 1 cut(s) 39
FaeI CATG 1 cut(s) 43
FaiI YATR 2 cut(s) 41, 128
FatI CATG 1 cut(s) 39
Fnu4HI GCNGC 1 cut(s) 60
FriOI GRGCYC 1 cut(s) 99
Fsp4HI GCNGC 1 cut(s) 60
GlaI GCGC 1 cut(s) 211
GluI GCNGC 1 cut(s) 60
GsaI CCCAGC 1 cut(s) 209
HaeII RGCGCY 1 cut(s) 213
HhaI GCGC 1 cut(s) 212
Hin1II CATG 1 cut(s) 43
Hin6I GCGC 1 cut(s) 210
HinP1I GCGC 1 cut(s) 210
HinfI GANTC 1 cut(s) 175
HphI GGTGA 1 cut(s) 37
Hpy166II GTNNAC 2 cut(s) 48, 179
Hpy188I TCNGA 2 cut(s) 94, 167
Hpy188III TCNNGA 4 cut(s) 73, 107, 185, 199
Hpy8I GTNNAC 2 cut(s) 48, 179
HpyAV CCTTC 1 cut(s) 134
HpyCH4III ACNGT 3 cut(s) 52, 132, 230
HpyCH4V TGCA 2 cut(s) 137, 221
HpyF10VI GCNNNNNNNGC 1 cut(s) 218
HpyF3I CTNAG 2 cut(s) 183, 234
Hsp92II CATG 1 cut(s) 43
HspAI GCGC 1 cut(s) 210
Kzo9I GATC 1 cut(s) 167
LmnI GCTCC 1 cut(s) 95
LpnPI CCDG 3 cut(s) 8, 170, 191
Lsp1109I GCAGC 1 cut(s) 46
LweI GCATC 1 cut(s) 208
MaeIII GTNAC 1 cut(s) 52
MalI GATC 1 cut(s) 169
MboI GATC 1 cut(s) 167
MhlI GDGCHC 1 cut(s) 99
MlyI GAGTC 1 cut(s) 184
MnlI CCTC 4 cut(s) 21, 24, 80, 192
MseI TTAA 2 cut(s) 117, 150
MwoI GCNNNNNNNGC 1 cut(s) 218
NcoI CCATGG 1 cut(s) 39
NdeII GATC 1 cut(s) 167
NlaIII CATG 1 cut(s) 43
NlaIV GGNNCC 1 cut(s) 91
NmuCI GTSAC 1 cut(s) 52
PkrI GCNGC 1 cut(s) 61
PleI GAGTC 1 cut(s) 183
PpsI GAGTC 1 cut(s) 183
PspFI CCCAGC 1 cut(s) 205
PspN4I GGNNCC 1 cut(s) 91
PspPI GGNCC 1 cut(s) 226
PstI CTGCAG 1 cut(s) 139
SaqAI TTAA 2 cut(s) 117, 150
SatI GCNGC 1 cut(s) 60
Sau3AI GATC 1 cut(s) 167
Sau96I GGNCC 1 cut(s) 226
SchI GAGTC 1 cut(s) 184
SduI GDGCHC 1 cut(s) 99
SetI ASST 4 cut(s) 127, 145, 184, 207
SfaNI GCATC 1 cut(s) 208
SfcI CTRYAG 1 cut(s) 135
SgeI CNNG 8 cut(s) 35, 52, 85, 106, 119, 197, 211, 218
SinI GGWCC 1 cut(s) 226
StyI CCWWGG 1 cut(s) 39
TaaI ACNGT 3 cut(s) 52, 132, 230
TaqI TCGA 2 cut(s) 108, 198
Tru1I TTAA 2 cut(s) 117, 150
Tru9I TTAA 2 cut(s) 117, 150
TscAI CASTG 2 cut(s) 55, 61
TseFI GTSAC 1 cut(s) 52
TseI GCWGC 1 cut(s) 59
Tsp45I GTSAC 1 cut(s) 52
TspDTI ATGAA 2 cut(s) 17, 148
TspRI CASTG 2 cut(s) 55, 61
VpaK11BI GGWCC 1 cut(s) 226
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.