MD07G1229300.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0009039
Physical Location & Seq
Reverse (-)
30432241 .. 30434343
2103 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1229300.v1.1.491

Sequence Viewer

Length: 612 bp
ATGCGCACGTTAGCATCCAAGCTCGCCACTTATTCAAAGTATTACCGTACAAGAAACCCGGTGCGCAATGCGCCTTCCCGCAATTTCTCAACTTACAACGGAAGAGATGAGCTCTCTCTTGAAGAGGAAGCTGAAAGAAAAATTGGGTGGATGTTGAAACTGATATTTGCTGGGACTGCAACTTGCGTAGCTTACAACATAATGCCTTATATGGGTGATACTTTGTTGCAACAGTCCGTGTCGTTGTTGACAGTCAAGGATCCGTTGTTTAAGAGAATGGGGGCTTCTCGATTAGCTCAGTTTGCGGTTGATGATGAAAGGAGGATGAAAATAGTGGAGATGGGTGGTGCTCGAGAGCTGGTGAATATGTTGGGCGGTGCTAAAGATGACCGCACAAGAAAGGAGGCTTTGAAAGCTCTTTCTGCTCTGTCACCTTCAGATAAAGCTCTTGGAGCTTTACACCAGGCTGGGGCAATCTCGGTTATCAGATCTAGCCCGGATTCCCTGGAGAATGCTGAAATTGAGAAATACAAGTCAAGCTTACTCAGAAGATTCCAAGATCTGAGGTACGATTTTCCAACCGCAGATGTTTCAAGGTCTTTAAACTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

204

Amino Acids

22.75

Weight (kDa)

9.61

Isoelectric Point (pI)

49.57

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 2 cut(s) 5, 65
AciI CCGC 5 cut(s) 79, 305, 375, 391, 582
AclWI GGATC 2 cut(s) 254, 267
AcuI CTGAAG 1 cut(s) 420
AfaI GTAC 2 cut(s) 49, 569
AfiI CCNNNNNNNGG 2 cut(s) 212, 469
AgsI TTSAA 5 cut(s) 36, 122, 157, 412, 594
AjnI CCWGG 2 cut(s) 462, 504
AjuI GAANNNNNNNTTGG 2 cut(s) 126, 158
Alw21I GWGCWC 2 cut(s) 114, 352
AlwI GGATC 2 cut(s) 254, 267
Ama87I CYCGRG 1 cut(s) 351
AspLEI GCGC 3 cut(s) 6, 66, 73
AsuC2I CCSGG 2 cut(s) 59, 497
AsuHPI GGTGA 3 cut(s) 227, 373, 423
AvaI CYCGRG 1 cut(s) 351
BamHI GGATCC 1 cut(s) 259
BanII GRGCYC 1 cut(s) 114
Bbv12I GWGCWC 2 cut(s) 114, 352
BccI CCATC 1 cut(s) 334
BciT130I CCWGG 2 cut(s) 464, 506
BcnI CCSGG 2 cut(s) 59, 497
BfaI CTAG 1 cut(s) 492
BglII AGATCT 2 cut(s) 488, 559
Bme1390I CCNGG 4 cut(s) 59, 464, 497, 506
BmeT110I CYCGRG 1 cut(s) 351
BmiI GGNNCC 1 cut(s) 261
BmrFI CCNGG 4 cut(s) 59, 464, 497, 506
BmsI GCATC 1 cut(s) 23
BplI GAGNNNNNCTC 2 cut(s) 96, 128
BpmI CTGGAG 1 cut(s) 527
BpuMI CCSGG 2 cut(s) 59, 497
BsaJI CCNNGG 1 cut(s) 504
Bsc4I CCNNNNNNNGG 2 cut(s) 212, 469
Bse3DI GCAATG 1 cut(s) 73
BseBI CCWGG 2 cut(s) 464, 506
BseDI CCNNGG 1 cut(s) 504
BseGI GGATG 3 cut(s) 14, 156, 330
BseLI CCNNNNNNNGG 2 cut(s) 212, 469
BseMI GCAATG 1 cut(s) 73
BseMII CTCAG 3 cut(s) 311, 554, 559
BseYI CCCAGC 2 cut(s) 170, 467
BsiHKAI GWGCWC 2 cut(s) 114, 352
BsiHKCI CYCGRG 1 cut(s) 351
BsiSI CCGG 2 cut(s) 59, 497
BslFI GGGAC 1 cut(s) 187
BslI CCNNNNNNNGG 2 cut(s) 212, 469
BsmFI GGGAC 1 cut(s) 187
BsmI GAATGC 1 cut(s) 517
BsoBI CYCGRG 1 cut(s) 351
Bsp1286I GDGCHC 2 cut(s) 114, 352
Bsp143I GATC 3 cut(s) 259, 488, 559
BspACI CCGC 5 cut(s) 79, 305, 375, 391, 582
BspCNI CTCAG 3 cut(s) 310, 555, 558
BspLI GGNNCC 1 cut(s) 261
BspPI GGATC 2 cut(s) 254, 267
BsrDI GCAATG 1 cut(s) 73
BssECI CCNNGG 1 cut(s) 504
BssMI GATC 3 cut(s) 259, 488, 559
Bst2UI CCWGG 2 cut(s) 464, 506
Bst4CI ACNGT 3 cut(s) 47, 234, 253
Bst6I CTCTTC 2 cut(s) 97, 117
BstC8I GCNNGC 1 cut(s) 24
BstDEI CTNAG 3 cut(s) 297, 545, 563
BstF5I GGATG 3 cut(s) 14, 156, 330
BstHHI GCGC 3 cut(s) 6, 66, 73
BstKTI GATC 3 cut(s) 262, 491, 562
BstMBI GATC 3 cut(s) 259, 488, 559
BstMWI GCNNNNNNNGC 6 cut(s) 70, 176, 302, 413, 422, 452
BstNI CCWGG 2 cut(s) 464, 506
BstSCI CCNGG 4 cut(s) 57, 462, 495, 504
BstX2I RGATCY 3 cut(s) 259, 488, 559
BstYI RGATCY 3 cut(s) 259, 488, 559
BtsCI GGATG 3 cut(s) 14, 156, 330
Cac8I GCNNGC 1 cut(s) 24
CfoI GCGC 3 cut(s) 6, 66, 73
Csp6I GTAC 2 cut(s) 48, 568
CviQI GTAC 2 cut(s) 48, 568
DdeI CTNAG 3 cut(s) 297, 545, 563
DpnI GATC 3 cut(s) 261, 490, 561
DpnII GATC 3 cut(s) 259, 488, 559
DraI TTTAAA 1 cut(s) 603
Eam1104I CTCTTC 2 cut(s) 97, 117
EarI CTCTTC 2 cut(s) 97, 117
Ecl136II GAGCTC 1 cut(s) 112
Eco24I GRGCYC 1 cut(s) 114
Eco53kI GAGCTC 1 cut(s) 112
Eco57I CTGAAG 1 cut(s) 420
Eco88I CYCGRG 1 cut(s) 351
EcoICRI GAGCTC 1 cut(s) 112
EcoRII CCWGG 2 cut(s) 462, 504
EcoT38I GRGCYC 1 cut(s) 114
FaiI YATR 4 cut(s) 200, 210, 212, 368
FalI AAGNNNNNCTT 2 cut(s) 524, 556
FaqI GGGAC 1 cut(s) 187
FauI CCCGC 1 cut(s) 86
FokI GGATG 2 cut(s) 163, 337
FriOI GRGCYC 1 cut(s) 114
FspAI RTGCGCAY 1 cut(s) 5
FspBI CTAG 1 cut(s) 492
FspI TGCGCA 2 cut(s) 5, 65
GlaI GCGC 3 cut(s) 5, 65, 72
GsaI CCCAGC 2 cut(s) 174, 471
GsuI CTGGAG 1 cut(s) 527
HapII CCGG 2 cut(s) 59, 497
HhaI GCGC 3 cut(s) 6, 66, 73
Hin6I GCGC 3 cut(s) 4, 64, 71
HinP1I GCGC 3 cut(s) 4, 64, 71
HincII GTYRAC 1 cut(s) 249
HindII GTYRAC 1 cut(s) 249
HindIII AAGCTT 1 cut(s) 538
HinfI GANTC 2 cut(s) 500, 552
HpaII CCGG 2 cut(s) 59, 497
HphI GGTGA 3 cut(s) 227, 373, 423
Hpy166II GTNNAC 1 cut(s) 249
Hpy188I TCNGA 4 cut(s) 439, 488, 548, 564
Hpy188III TCNNGA 3 cut(s) 119, 288, 353
Hpy8I GTNNAC 1 cut(s) 249
HpyAV CCTTC 2 cut(s) 84, 444
HpyCH4III ACNGT 3 cut(s) 47, 234, 253
HpyCH4IV ACGT 1 cut(s) 8
HpyCH4V TGCA 2 cut(s) 179, 229
HpyF10VI GCNNNNNNNGC 6 cut(s) 70, 176, 302, 413, 422, 452
HpyF3I CTNAG 3 cut(s) 297, 545, 563
HpySE526I ACGT 1 cut(s) 8
HspAI GCGC 3 cut(s) 4, 64, 71
Kzo9I GATC 3 cut(s) 259, 488, 559
LmnI GCTCC 1 cut(s) 452
LpnPI CCDG 9 cut(s) 72, 156, 344, 449, 453, 476, 491, 510, 518
LweI GCATC 1 cut(s) 23
MaeI CTAG 1 cut(s) 492
MaeII ACGT 1 cut(s) 8
MaeIII GTNAC 1 cut(s) 429
MalI GATC 3 cut(s) 261, 490, 561
MboI GATC 3 cut(s) 259, 488, 559
MboII GAAGA 3 cut(s) 114, 134, 561
MflI RGATCY 3 cut(s) 259, 488, 559
MhlI GDGCHC 2 cut(s) 114, 352
MluCI AATT 3 cut(s) 82, 141, 519
MmeI TCCRAC 1 cut(s) 602
MnlI CCTC 4 cut(s) 118, 315, 397, 558
MseI TTAA 3 cut(s) 270, 602, 610
MspI CCGG 2 cut(s) 59, 497
MspR9I CCNGG 4 cut(s) 59, 464, 497, 506
Mva1269I GAATGC 1 cut(s) 517
MvaI CCWGG 2 cut(s) 464, 506
MwoI GCNNNNNNNGC 6 cut(s) 70, 176, 302, 413, 422, 452
NciI CCSGG 2 cut(s) 59, 497
NdeII GATC 3 cut(s) 259, 488, 559
NlaIV GGNNCC 1 cut(s) 261
NmuCI GTSAC 1 cut(s) 429
NsbI TGCGCA 2 cut(s) 5, 65
PaeR7I CTCGAG 1 cut(s) 351
PctI GAATGC 1 cut(s) 517
PfeI GAWTC 2 cut(s) 500, 552
Psp124BI GAGCTC 1 cut(s) 114
Psp6I CCWGG 2 cut(s) 462, 504
PspFI CCCAGC 2 cut(s) 170, 467
PspGI CCWGG 2 cut(s) 462, 504
PspN4I GGNNCC 1 cut(s) 261
PsuI RGATCY 3 cut(s) 259, 488, 559
RsaI GTAC 2 cut(s) 49, 569
RsaNI GTAC 2 cut(s) 48, 568
SacI GAGCTC 1 cut(s) 114
SaqAI TTAA 3 cut(s) 270, 602, 610
Sau3AI GATC 3 cut(s) 259, 488, 559
ScrFI CCNGG 4 cut(s) 59, 464, 497, 506
SduI GDGCHC 2 cut(s) 114, 352
SfaNI GCATC 1 cut(s) 23
Sfr274I CTCGAG 1 cut(s) 351
SlaI CTCGAG 1 cut(s) 351
SmlI CTYRAG 1 cut(s) 351
SmoI CTYRAG 1 cut(s) 351
Sse9I AATT 3 cut(s) 82, 141, 519
SsiI CCGC 5 cut(s) 79, 305, 375, 391, 582
SspMI CTAG 1 cut(s) 492
SstI GAGCTC 1 cut(s) 114
StyD4I CCNGG 4 cut(s) 57, 462, 495, 504
TaaI ACNGT 3 cut(s) 47, 234, 253
TaiI ACGT 1 cut(s) 11
TaqI TCGA 2 cut(s) 289, 352
TasI AATT 3 cut(s) 82, 141, 519
TfiI GAWTC 2 cut(s) 500, 552
Tru1I TTAA 3 cut(s) 270, 602, 610
Tru9I TTAA 3 cut(s) 270, 602, 610
TseFI GTSAC 1 cut(s) 429
Tsp45I GTSAC 1 cut(s) 429
TspDTI ATGAA 2 cut(s) 330, 341
TspGWI ACGGA 3 cut(s) 114, 226, 252
XhoI CTCGAG 1 cut(s) 351
XspI CTAG 1 cut(s) 492
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.