pycom01g17720

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0009039
Physical Location & Seq
Reverse (-)
16888063 .. 16889789
1727 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom01g17720.1

Sequence Viewer

Length: 612 bp
ATGCGCACTTTAGCATCCAAGCTCACCGCTTATTCGAAGTATTACTGTACAAGAAGCCCGGTGCGCAATGCGCCGCCCCGCAATTTCTCAACTCACAACGGCAGAGATGAACTCTCCCTCGAAGAGGAAGCTGAAAGAAAAATTGGATGGATGTTGAAGCTGATATTTGCTGGGACTGCAACTTGCGTAGCTTACAACATCATGCCTTATATGGGGGATACTTTGTTGCAACAGTCCGTTTCGCTGTTGACAGTCAAGGATCCTTTGTTTAAGAGAATGGGGGCTTCTCGATTGGCTCAGTTTGCGGTTGATGATGAAAGGAGAATGAAAATTGTGGAGATGGGTGGTGCTCAAGAGCTCGTGAATATGTTGGGGGCCGCTAAAGATGATAGCACAAGGAAGGAGGCTTTGAAAGCTCTTACCGCTCTATCACCCTCAGATAAAGCTCTTGGAGCTTTACACCAAGCTGGGGCTATCCCAGTTATCAGATCCACCCCTGATTCTCTGGAGAATGCTGAAATCGAGAAATACAAGTTGGGATTACTCAGGAGATTCCAAGATCAGAGATACGATTTTCCAACCGCAGATGTTTCAAGGTCTTTAGATTATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

204

Amino Acids

22.7

Weight (kDa)

9.02

Isoelectric Point (pI)

52.76

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 2 cut(s) 5, 65
AccBSI CCGCTC 1 cut(s) 425
AciI CCGC 7 cut(s) 27, 74, 79, 305, 378, 423, 582
AclWI GGATC 3 cut(s) 254, 267, 483
AfaI GTAC 1 cut(s) 49
AfiI CCNNNNNNNGG 3 cut(s) 124, 212, 469
AgsI TTSAA 3 cut(s) 157, 412, 594
AjuI GAANNNNNNNTTGG 4 cut(s) 126, 158, 518, 550
AluBI AGCT 9 cut(s) 22, 131, 160, 191, 358, 416, 446, 455, 467
AluI AGCT 9 cut(s) 22, 131, 160, 191, 358, 416, 446, 455, 467
Alw21I GWGCWC 2 cut(s) 352, 360
AlwI GGATC 3 cut(s) 254, 267, 483
AoxI GGCC 1 cut(s) 375
AspLEI GCGC 3 cut(s) 6, 66, 73
AspS9I GGNCC 1 cut(s) 375
AsuC2I CCSGG 1 cut(s) 59
AsuHPI GGTGA 2 cut(s) 16, 423
AsuII TTCGAA 1 cut(s) 35
BamHI GGATCC 1 cut(s) 259
BanII GRGCYC 1 cut(s) 360
BauI CACGAG 1 cut(s) 359
Bbv12I GWGCWC 2 cut(s) 352, 360
BccI CCATC 2 cut(s) 141, 334
BceAI ACGGC 1 cut(s) 115
BciVI GTATCC 1 cut(s) 211
BcnI CCSGG 1 cut(s) 59
BfuI GTATCC 1 cut(s) 211
BisI GCNGC 2 cut(s) 74, 378
BlsI GCNGC 2 cut(s) 75, 379
Bme1390I CCNGG 1 cut(s) 59
BmgT120I GGNCC 1 cut(s) 375
BmiI GGNNCC 2 cut(s) 261, 376
BmrFI CCNGG 1 cut(s) 59
BmrI ACTGGG 1 cut(s) 473
BmsI GCATC 1 cut(s) 23
BmuI ACTGGG 1 cut(s) 473
BplI GAGNNNNNCTC 2 cut(s) 96, 128
BpmI CTGGAG 1 cut(s) 527
Bpu14I TTCGAA 1 cut(s) 35
BpuEI CTTGAG 1 cut(s) 336
BpuMI CCSGG 1 cut(s) 59
Bsc4I CCNNNNNNNGG 3 cut(s) 124, 212, 469
Bse1I ACTGG 1 cut(s) 479
Bse3DI GCAATG 1 cut(s) 73
BseGI GGATG 3 cut(s) 14, 152, 156
BseLI CCNNNNNNNGG 3 cut(s) 124, 212, 469
BseMI GCAATG 1 cut(s) 73
BseMII CTCAG 3 cut(s) 311, 450, 559
BseNI ACTGG 1 cut(s) 479
BseYI CCCAGC 2 cut(s) 170, 467
BshFI GGCC 1 cut(s) 377
BsiHKAI GWGCWC 2 cut(s) 352, 360
BsiSI CCGG 1 cut(s) 59
BslFI GGGAC 1 cut(s) 187
BslI CCNNNNNNNGG 3 cut(s) 124, 212, 469
BsmFI GGGAC 1 cut(s) 187
BsmI GAATGC 1 cut(s) 517
BsnI GGCC 1 cut(s) 377
Bsp119I TTCGAA 1 cut(s) 35
Bsp1286I GDGCHC 2 cut(s) 352, 360
Bsp1407I TGTACA 1 cut(s) 47
Bsp143I GATC 3 cut(s) 259, 488, 559
BspACI CCGC 7 cut(s) 27, 74, 79, 305, 378, 423, 582
BspANI GGCC 1 cut(s) 377
BspCNI CTCAG 3 cut(s) 310, 449, 558
BspLI GGNNCC 2 cut(s) 261, 376
BspPI GGATC 3 cut(s) 254, 267, 483
BspT104I TTCGAA 1 cut(s) 35
BsrBI CCGCTC 1 cut(s) 425
BsrDI GCAATG 1 cut(s) 73
BsrGI TGTACA 1 cut(s) 47
BsrI ACTGG 1 cut(s) 479
BssMI GATC 3 cut(s) 259, 488, 559
BssSI CACGAG 1 cut(s) 359
Bst2BI CACGAG 1 cut(s) 359
Bst4CI ACNGT 3 cut(s) 47, 234, 253
Bst6I CTCTTC 1 cut(s) 117
BstAUI TGTACA 1 cut(s) 47
BstBI TTCGAA 1 cut(s) 35
BstDEI CTNAG 3 cut(s) 297, 436, 545
BstENI CCTNNNNNAGG 1 cut(s) 122
BstF5I GGATG 3 cut(s) 14, 152, 156
BstHHI GCGC 3 cut(s) 6, 66, 73
BstKTI GATC 3 cut(s) 262, 491, 562
BstMBI GATC 3 cut(s) 259, 488, 559
BstMWI GCNNNNNNNGC 7 cut(s) 63, 70, 176, 302, 413, 422, 452
BstSCI CCNGG 1 cut(s) 57
BstX2I RGATCY 2 cut(s) 259, 488
BstYI RGATCY 2 cut(s) 259, 488
BsuI GTATCC 1 cut(s) 211
BsuRI GGCC 1 cut(s) 377
BtsCI GGATG 3 cut(s) 14, 152, 156
CfoI GCGC 3 cut(s) 6, 66, 73
Cfr13I GGNCC 1 cut(s) 375
Csp6I GTAC 1 cut(s) 48
CviAII CATG 1 cut(s) 202
CviQI GTAC 1 cut(s) 48
DdeI CTNAG 3 cut(s) 297, 436, 545
DpnI GATC 3 cut(s) 261, 490, 561
DpnII GATC 3 cut(s) 259, 488, 559
Eam1104I CTCTTC 1 cut(s) 117
EarI CTCTTC 1 cut(s) 117
Ecl136II GAGCTC 1 cut(s) 358
Eco24I GRGCYC 1 cut(s) 360
Eco53kI GAGCTC 1 cut(s) 358
EcoICRI GAGCTC 1 cut(s) 358
EcoNI CCTNNNNNAGG 1 cut(s) 122
EcoT38I GRGCYC 1 cut(s) 360
FaeI CATG 1 cut(s) 205
FaiI YATR 4 cut(s) 203, 210, 212, 368
FaqI GGGAC 1 cut(s) 187
FatI CATG 1 cut(s) 201
FauI CCCGC 1 cut(s) 86
Fnu4HI GCNGC 2 cut(s) 74, 378
FokI GGATG 2 cut(s) 159, 163
FriOI GRGCYC 1 cut(s) 360
Fsp4HI GCNGC 2 cut(s) 74, 378
FspAI RTGCGCAY 1 cut(s) 5
FspI TGCGCA 2 cut(s) 5, 65
GlaI GCGC 3 cut(s) 5, 65, 72
GluI GCNGC 2 cut(s) 74, 378
GsaI CCCAGC 2 cut(s) 174, 471
GsuI CTGGAG 1 cut(s) 527
HaeIII GGCC 1 cut(s) 377
HapII CCGG 1 cut(s) 59
HhaI GCGC 3 cut(s) 6, 66, 73
Hin1II CATG 1 cut(s) 205
Hin6I GCGC 3 cut(s) 4, 64, 71
HinP1I GCGC 3 cut(s) 4, 64, 71
HincII GTYRAC 1 cut(s) 249
HindII GTYRAC 1 cut(s) 249
HinfI GANTC 2 cut(s) 500, 552
HpaII CCGG 1 cut(s) 59
HphI GGTGA 2 cut(s) 16, 423
Hpy166II GTNNAC 1 cut(s) 249
Hpy188I TCNGA 3 cut(s) 439, 488, 564
Hpy188III TCNNGA 6 cut(s) 288, 353, 361, 506, 523, 547
Hpy8I GTNNAC 1 cut(s) 249
HpyAV CCTTC 1 cut(s) 394
HpyCH4III ACNGT 3 cut(s) 47, 234, 253
HpyCH4V TGCA 2 cut(s) 179, 229
HpyF10VI GCNNNNNNNGC 7 cut(s) 63, 70, 176, 302, 413, 422, 452
HpyF3I CTNAG 3 cut(s) 297, 436, 545
Hsp92II CATG 1 cut(s) 205
HspAI GCGC 3 cut(s) 4, 64, 71
Kzo9I GATC 3 cut(s) 259, 488, 559
LmnI GCTCC 1 cut(s) 452
LpnPI CCDG 7 cut(s) 72, 156, 453, 491, 492, 510, 532
LweI GCATC 1 cut(s) 23
MalI GATC 3 cut(s) 261, 490, 561
MbiI CCGCTC 1 cut(s) 425
MboI GATC 3 cut(s) 259, 488, 559
MboII GAAGA 1 cut(s) 134
MflI RGATCY 2 cut(s) 259, 488
MhlI GDGCHC 2 cut(s) 352, 360
MluCI AATT 3 cut(s) 82, 141, 330
MmeI TCCRAC 1 cut(s) 602
MnlI CCTC 4 cut(s) 118, 128, 397, 445
MseI TTAA 2 cut(s) 270, 610
MspI CCGG 1 cut(s) 59
MspR9I CCNGG 1 cut(s) 59
Mva1269I GAATGC 1 cut(s) 517
MwoI GCNNNNNNNGC 7 cut(s) 63, 70, 176, 302, 413, 422, 452
NciI CCSGG 1 cut(s) 59
NdeII GATC 3 cut(s) 259, 488, 559
NlaIII CATG 1 cut(s) 205
NlaIV GGNNCC 2 cut(s) 261, 376
NsbI TGCGCA 2 cut(s) 5, 65
NspV TTCGAA 1 cut(s) 35
PctI GAATGC 1 cut(s) 517
PfeI GAWTC 2 cut(s) 500, 552
PkrI GCNGC 2 cut(s) 75, 379
Psp124BI GAGCTC 1 cut(s) 360
PspFI CCCAGC 2 cut(s) 170, 467
PspN4I GGNNCC 2 cut(s) 261, 376
PspPI GGNCC 1 cut(s) 375
PsuI RGATCY 2 cut(s) 259, 488
RsaI GTAC 1 cut(s) 49
RsaNI GTAC 1 cut(s) 48
SacI GAGCTC 1 cut(s) 360
SaqAI TTAA 2 cut(s) 270, 610
SatI GCNGC 2 cut(s) 74, 378
Sau3AI GATC 3 cut(s) 259, 488, 559
Sau96I GGNCC 1 cut(s) 375
ScrFI CCNGG 1 cut(s) 59
SduI GDGCHC 2 cut(s) 352, 360
SfaNI GCATC 1 cut(s) 23
SfuI TTCGAA 1 cut(s) 35
SmlI CTYRAG 1 cut(s) 351
SmoI CTYRAG 1 cut(s) 351
Sse9I AATT 3 cut(s) 82, 141, 330
SsiI CCGC 7 cut(s) 27, 74, 79, 305, 378, 423, 582
SstI GAGCTC 1 cut(s) 360
StyD4I CCNGG 1 cut(s) 57
TaaI ACNGT 3 cut(s) 47, 234, 253
TaqI TCGA 4 cut(s) 35, 120, 289, 522
TasI AATT 3 cut(s) 82, 141, 330
TatI WGTACW 1 cut(s) 47
TauI GCSGC 2 cut(s) 76, 380
TfiI GAWTC 2 cut(s) 500, 552
Tru1I TTAA 2 cut(s) 270, 610
Tru9I TTAA 2 cut(s) 270, 610
TspDTI ATGAA 3 cut(s) 123, 330, 341
TspGWI ACGGA 1 cut(s) 226
XagI CCTNNNNNAGG 1 cut(s) 122
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.