MD07G1276200.v1.1

Belongs to the MT-A70-like family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0005856
Physical Location & Seq
Reverse (-)
34180608 .. 34186313
5706 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1276200.v1.1.491

Sequence Viewer

Length: 3285 bp
ATGAAGAAAAAACAAGAAGAGAGCAGTTTGGAAAAGTTGAGCAGTTGGTATCAGGACGGCGAATTAGAGAACAGGCAAGATGGGGGAGATAAGTCGGGAGGTAGAGGGTCAGTTCGAACCGAAGAAAGTGATAAAAGGAAAATGAGCTCAAAGCTTACACAGCATGAGGATTCTCAGAGTAAAAGTAAGAATAAAGAGGAAAGACCCTATGATGGAGAGCTTGAAAAGACACTTGGTAGAGACTCCAGGCATTCGGAGAGCAAGGAAAGTAGCCATGAGAGGACGCATGGTTCTTCTGAACAGGTGAAGACCTCAAGAAGAAGATGGGATGAATCAGAAGGTGGGAGAAAGGCAGAAGAAAGTCATATTGAAAAATCTGATTCAAGAAGCAGTAAGCCTTCTGATCCTAAGTATGAGAGTTCTAAGGAAAAGAGTGTCACTGTGAGAAATGAACCCAGTGACAGGAGAATCCGTGGTGTGGATTCAAACAGTGATAGGCCTACCAAATCTAACAGGGAAGAGAGAAAACCTGATTTGGAAAAGAGTAAGATTAAAAGCAGGACCGAAACACTGGAAGAAGGCAACAGTGATAGTCCTGTCACTCGTGAAGATAGATCACACAGGGAGAAAACTGAAAAACATAGACAGCAGAGAACTCCAACTGGTCGGGATGTTGCTGAAAGCAAGGAGAGGTCTTTGAATGCAGATGAAGAAGCAAATGCGGGGACGAAAGACAAGGGTCCTAGAGAAGTAGGAAGTACCACCAGGTCAAGGACACCTGATAGGAGTGGGAGGCGTTATCAGGATTCAGAATACGATTATGACAGGAATTTTAATCTTAAACGGAAGGAACTGGAAAAAGATGGTTATAGGAATGATCGACCTAAAGGCAGAGATGATGACTGGACTGACAGGAGTAGGGATCGGGAAGGTTCCAAAGAGAACTGGAAAAGAAGGCAACCCAGCAGTAGTGAAAAAGACTCCAAAAATGGGGACATCAATTATGATCAGGGTAGGGAGTGGGAGCTGCCACGACACGGTCGTGAGAGGACTGACAGTGAAAGGCCTCATGGTCGGTCTGGTAATAGGAAAGATGGAAGCCGTGGTGAAGCTGTGAAGACCTCATCAAATTTTGGAATTTCAAATGAAAATTATGATGTGATTGAGATCCAAACTAAACCAATAGATTACGGAAGAGTAGAATCAGCATCCAACTTTGCCAGGAGGACCGAAGCTGGTCAGCAATCTGATGGGAAATCAGCACCAAGTGAGGAAGAGTGGGCATATCTGCAGGATGACAGAACAAGGAGTGATATGTATGGATCGGGGCCACCTAGGGAAGATTCAAAGGAAAGATATACAGATGATATTAACTCCTTGCGAGATCAGAATTCATGGAGGGAGGACTCTGATGCCCATGGAGGCAAAGGAAGAGGACAGAAAGGTTCCATGCCTGGGTGTGGTACTGGTGGCCAAAGTTCTGGGGGTGGTTCACAGCCTCCATATGGAAATGCGGAGCCAGGTTCCTTCAATAGAAATGCTTCGCAAGGGATGAAAGGGGGGAGAGTTGGTAGAGGAGGAAGGGGTAGGCTTACTGGAAGAGATAGCCAACCGGTAGGAATTCCACTTCCAATAATGGGATCGCCGTTTGGCCCTCTTGGAATGCCACCACCTGGACCTATGCAGCCACTTACACCTAGCATGTCACCTGCTCCAGGTCCACCGATGTCCCATGGTGTCTTCATTCCTCCATTTTCTCCTCCCGTGTGGCCTGGGGCTCGGGGAGTTGATATGAATATGTTAGGTGTCCCACCTGGTCTCTCCTCAGTCCCTCCTGGACCATCAGGCCCAAGATTTCCTACTAATATGGGTACTCCGACAAACCCTGCCATGTTTTTTAACCAATCTGGCCATGGAAGAGGAGTTCCTCCAAACATATCTGGCCCTGGTTTTAATTCTGCAGGACCAATGGGACGAGGAACTCCGGCTGATAAAAATCAAGGTGGTTGGTTTCCTCATAAAAGTAGTGGACCTCCTGGTAAAGCTCCTTCCAGAGGAGAGCAGAATGATTACTCTCAAAATTTTGTCGATACCGGTATGCGACCCCAGAATTTCATCAGGGAGCTAGAGCTTACAAATGTTGTGGAGGATTACCCCAAGCTGAGGGAGCTTATACAGAAAAAGGATGAGATTGTGGCTCATTCTGCTTCACATCCCATGTATTACAAGTGTGACCTGAAAGAGTTTGAGCTATCCCCAGAGTTTTTTGGAACCAAGTTTGATGTCATTCTTGTGGATCCACCTTGGGAGGAATACGTTCATCGTGCTCCTGGTGTTGCTGACCACACAGAGTATTGGACATTTGAAGAAATAATGAATCTTAAGATTGAGGCAATAGCAGACACACCTTCTTTCATTTTTCTTTGGGTGGGTGATGGCATGGGTCTTGAGCAGGGTCGTCAATGTCTAAAAAAGTGGGGCTTTCGTAGGTGTGAAGATATATGTTGGGTGAAGACAAACAAAACTAATGCAACTCCTGGTTTGCGGCACGATGCTCATACTTTGTTTCAGCACTCAAAGGAACATTGCTTGATGGGAATAAAAGGCACTGTTCGTCGCAGCACTGATGGTCATATAATCCACGCCAACATTGATACAGATGTGATAATAGCCGAGGAACCCCCGTACGGTTCAACCGAAAAGCCTGAGGATATGTACAGGATTATTGAGCATTTTGCCCTTGGCCGCAGAAGGCTTGAGCTTTTTGGTGAAGACCACAACATTCGGTCTGGCTGGCTGACTGTTGGTAAAGGACTATCCTCATCGAATTTTAATACCGAGGGCTATATCAAGAACTTTGCTGACAAGGACGGGAAGGTTTGGCAAGGAGGGGGTGGACGAAACCCGCCTCCAGAGGCACCTCATCTTGTTGTGACAACTCCCGACATAGAAGCACTTCGGCCCAAGTCACCAATGAAAAACCAGCAGCAATTGCAGCAGCAGAATTCATCGTCCATTTCTCTGACACCGGCAAATTCCTCCAACAGAAGACCTGCCGGAAATTCGCCACAAAATCCAATTGCTCTTGGTATAAACCAAGAAGGTTCAAATTCCAATCCCTCAACTCCAGCTTCTTGGGGGCCTCCACCAATGGACGGTTTTAAAGGGAGAGACGGCAACAATATGTCGTCCGATGATAAGGTTTTTGATATGCATGTATACTCCGGACAGCCAAATGCAGAGTTTGTAGATTTTGAGTCTCACAGACATATGAATTTGTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MT-A70

Protein Analysis

1095

Amino Acids

122.07

Weight (kDa)

7.03

Isoelectric Point (pI)

58.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MT-A70 PF05063 760 - 937 5.9e-52 MT-A70
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1717
Acc36I ACCTGC 2 cut(s) 1717, 3061
AccB1I GGYRCC 1 cut(s) 2917
AccB7I CCANNNNNTGG 1 cut(s) 1673
AccI GTMKAC 1 cut(s) 3219
AccIII TCCGGA 1 cut(s) 3224
AciI CCGC 5 cut(s) 722, 1514, 2545, 2746, 2906
AclWI GGATC 7 cut(s) 398, 930, 1162, 1330, 1648, 2291, 2304
AcoI YGGCCR 3 cut(s) 1471, 1909, 2743
AdeI CACNNNGTG 1 cut(s) 1268
AfaI GTAC 5 cut(s) 760, 1465, 1873, 2687, 2717
AflII CTTAAG 1 cut(s) 2381
AgeI ACCGGT 2 cut(s) 1612, 2093
AjuI GAANNNNNNNTTGG 2 cut(s) 216, 248
AleI CACNNNNGTG 1 cut(s) 1041
AloI GAACNNNNNNTCC 4 cut(s) 274, 306, 1908, 1940
Alw21I GWGCWC 2 cut(s) 149, 2329
Alw26I GTCTC 4 cut(s) 234, 1823, 3165, 3264
AlwI GGATC 7 cut(s) 398, 930, 1162, 1330, 1648, 2291, 2304
Ama87I CYCGRG 1 cut(s) 1779
Aor13HI TCCGGA 1 cut(s) 3224
ApeKI GCWGC 6 cut(s) 1027, 1684, 2619, 2986, 2995, 2998
AsiGI ACCGGT 2 cut(s) 1612, 2093
Asp700I GAANNNNTTC 3 cut(s) 1540, 2316, 2955
AspA2I CCTAGG 1 cut(s) 1334
AsuHPI GGTGA 7 cut(s) 316, 1118, 1698, 2444, 2521, 2780, 2961
AsuII TTCGAA 1 cut(s) 115
AvaI CYCGRG 1 cut(s) 1779
AvaII GGWCC 8 cut(s) 561, 740, 1227, 1676, 1718, 1838, 1964, 2030
AvrII CCTAGG 1 cut(s) 1334
AxyI CCTNAGG 1 cut(s) 2706
BaeI ACNNNNGTAYC 2 cut(s) 2646, 2679
BalI TGGCCA 2 cut(s) 1473, 1911
BamHI GGATCC 1 cut(s) 2296
BanI GGYRCC 1 cut(s) 2917
BanII GRGCYC 2 cut(s) 149, 1780
BarI GAAGNNNNNNTAC 2 cut(s) 2032, 2064
BauI CACGAG 1 cut(s) 603
BbsI GAAGAC 6 cut(s) 314, 1124, 1732, 2519, 2778, 3055
Bbv12I GWGCWC 2 cut(s) 149, 2329
BbvCI CCTCAGC 1 cut(s) 2162
BbvI GCAGC 6 cut(s) 1014, 1696, 2631, 2998, 3007, 3010
BceAI ACGGC 4 cut(s) 73, 1086, 1630, 3190
BclI TGATCA 1 cut(s) 1006
BcoDI GTCTC 4 cut(s) 234, 1823, 3165, 3264
BfaI CTAG 4 cut(s) 744, 1335, 1698, 2126
BfmI CTRYAG 2 cut(s) 1289, 1959
BfrI CTTAAG 1 cut(s) 2381
BfuAI ACCTGC 2 cut(s) 1717, 3061
BisI GCNGC 8 cut(s) 1028, 1685, 2546, 2620, 2746, 2987, 2996, 2999
BlnI CCTAGG 1 cut(s) 1334
BlsI GCNGC 8 cut(s) 1029, 1686, 2547, 2621, 2747, 2988, 2997, 3000
Bme18I GGWCC 8 cut(s) 561, 740, 1227, 1676, 1718, 1838, 1964, 2030
BmeT110I CYCGRG 1 cut(s) 1779
BmrI ACTGGG 1 cut(s) 450
BmsI GCATC 3 cut(s) 1217, 1402, 2542
BmuI ACTGGG 1 cut(s) 450
BoxI GACNNNNGTC 1 cut(s) 738
BpiI GAAGAC 6 cut(s) 314, 1124, 1732, 2519, 2778, 3055
BpmI CTGGAG 4 cut(s) 229, 1698, 2895, 3111
Bpu10I CCTNAGC 1 cut(s) 2162
Bpu14I TTCGAA 1 cut(s) 115
BpuEI CTTGAG 3 cut(s) 298, 2468, 2777
BsaBI GATNNNNATC 2 cut(s) 1166, 1995
BsaI GGTCTC 1 cut(s) 1823
BsaWI WCCGGW 3 cut(s) 1612, 2093, 3224
BsaXI ACNNNNNCTCC 4 cut(s) 457, 487, 1555, 1585
Bse118I RCCGGY 3 cut(s) 1612, 2093, 3028
Bse1I ACTGG 8 cut(s) 456, 576, 667, 858, 908, 950, 1471, 1600
Bse21I CCTNAGG 1 cut(s) 2706
Bse3DI GCAATG 1 cut(s) 2584
Bse8I GATNNNNATC 2 cut(s) 1166, 1995
BseAI TCCGGA 1 cut(s) 3224
BseGI GGATG 7 cut(s) 334, 676, 1208, 1300, 1557, 2191, 2212
BseJI GATNNNNATC 2 cut(s) 1166, 1995
BseMI GCAATG 1 cut(s) 2584
BseMII CTCAG 4 cut(s) 188, 1839, 2153, 2697
BseNI ACTGG 8 cut(s) 456, 576, 667, 858, 908, 950, 1471, 1600
BseRI GAGGAG 5 cut(s) 1590, 1749, 1813, 1935, 2070
BseXI GCAGC 6 cut(s) 1014, 1696, 2631, 2998, 3007, 3010
BseYI CCCAGC 1 cut(s) 962
Bsh1285I CGRYCG 1 cut(s) 1042
BshNI GGYRCC 1 cut(s) 2917
BshTI ACCGGT 2 cut(s) 1612, 2093
BsiEI CGRYCG 1 cut(s) 1042
BsiHKAI GWGCWC 2 cut(s) 149, 2329
BsiHKCI CYCGRG 1 cut(s) 1779
BsiSI CCGG 6 cut(s) 1613, 1985, 2094, 3029, 3057, 3225
BsiWI CGTACG 1 cut(s) 2685
BslFI GGGAC 6 cut(s) 739, 1007, 1714, 1793, 1814, 1986
BsmAI GTCTC 4 cut(s) 234, 1823, 3165, 3264
BsmBI CGTCTC 1 cut(s) 3165
BsmFI GGGAC 6 cut(s) 739, 1007, 1714, 1793, 1814, 1986
BsmI GAATGC 3 cut(s) 250, 706, 1668
Bso31I GGTCTC 1 cut(s) 1823
BsoBI CYCGRG 1 cut(s) 1779
Bsp119I TTCGAA 1 cut(s) 115
Bsp1286I GDGCHC 3 cut(s) 149, 1780, 2329
Bsp13I TCCGGA 1 cut(s) 3224
Bsp1407I TGTACA 1 cut(s) 2715
Bsp19I CCATGG 3 cut(s) 1417, 1732, 1912
BspACI CCGC 5 cut(s) 722, 1514, 2545, 2746, 2906
BspCNI CTCAG 4 cut(s) 187, 1838, 2154, 2698
BspEI TCCGGA 1 cut(s) 3224
BspMAI CTGCAG 2 cut(s) 1293, 1963
BspMI ACCTGC 2 cut(s) 1717, 3061
BspPI GGATC 7 cut(s) 398, 930, 1162, 1330, 1648, 2291, 2304
BspT104I TTCGAA 1 cut(s) 115
BspT107I GGYRCC 1 cut(s) 2917
BspTI CTTAAG 1 cut(s) 2381
BspTNI GGTCTC 1 cut(s) 1823
BsrDI GCAATG 1 cut(s) 2584
BsrFI RCCGGY 3 cut(s) 1612, 2093, 3028
BsrGI TGTACA 1 cut(s) 2715
BsrI ACTGG 8 cut(s) 456, 576, 667, 858, 908, 950, 1471, 1600
BssAI RCCGGY 3 cut(s) 1612, 2093, 3028
BssNAI GTATAC 1 cut(s) 3220
BssSI CACGAG 1 cut(s) 603
BssT1I CCWWGG 6 cut(s) 1334, 1417, 1732, 1912, 2303, 2740
Bst1107I GTATAC 1 cut(s) 3220
Bst2BI CACGAG 1 cut(s) 603
Bst4CI ACNGT 9 cut(s) 442, 491, 587, 1040, 1058, 2611, 2690, 2803, 3158
Bst6I CTCTTC 7 cut(s) 12, 513, 1189, 1269, 1426, 1594, 1912
BstAFI CTTAAG 1 cut(s) 2381
BstAPI GCANNNNNTGC 1 cut(s) 2992
BstAUI TGTACA 1 cut(s) 2715
BstBI TTCGAA 1 cut(s) 115
BstC8I GCNNGC 1 cut(s) 2795
BstDEI CTNAG 6 cut(s) 174, 408, 423, 1825, 2162, 2706
BstDSI CCRYGG 5 cut(s) 472, 1102, 1417, 1732, 1912
BstENI CCTNNNNNAGG 2 cut(s) 1713, 2052
BstF5I GGATG 7 cut(s) 334, 676, 1208, 1300, 1557, 2191, 2212
BstMAI GTCTC 4 cut(s) 234, 1823, 3165, 3264
BstMCI CGRYCG 1 cut(s) 1042
BstMWI GCNNNNNNNGC 5 cut(s) 160, 2167, 2204, 2992, 2995
BstNSI RCATGY 2 cut(s) 1705, 3218
BstPAI GACNNNNGTC 1 cut(s) 738
BstSFI CTRYAG 2 cut(s) 1289, 1959
BstV1I GCAGC 6 cut(s) 1014, 1696, 2631, 2998, 3007, 3010
BstV2I GAAGAC 6 cut(s) 314, 1124, 1732, 2519, 2778, 3055
BstX2I RGATCY 2 cut(s) 1167, 2296
BstXI CCANNNNNNTGG 2 cut(s) 1481, 3135
BstYI RGATCY 2 cut(s) 1167, 2296
BstZ17I GTATAC 1 cut(s) 3220
Bsu36I CCTNAGG 1 cut(s) 2706
BtgI CCRYGG 5 cut(s) 472, 1102, 1417, 1732, 1912
BtsCI GGATG 7 cut(s) 334, 676, 1208, 1300, 1557, 2191, 2212
BtsIMutI CAGTG 8 cut(s) 438, 463, 496, 569, 592, 1063, 2607, 2622
BveI ACCTGC 2 cut(s) 1717, 3061
Cac8I GCNNGC 1 cut(s) 2795
Cfr10I RCCGGY 3 cut(s) 1612, 2093, 3028
CseI GACGC 1 cut(s) 292
CsiI ACCWGGT 2 cut(s) 764, 1813
Csp6I GTAC 5 cut(s) 759, 1464, 1872, 2686, 2716
CspAI ACCGGT 2 cut(s) 1612, 2093
CspCI CAANNNNNGTGG 2 cut(s) 2124, 2159
CviQI GTAC 5 cut(s) 759, 1464, 1872, 2686, 2716
DdeI CTNAG 6 cut(s) 174, 408, 423, 1825, 2162, 2706
DraI TTTAAA 1 cut(s) 3163
DraIII CACNNNGTG 1 cut(s) 1268
EaeI YGGCCR 3 cut(s) 1471, 1909, 2743
Eam1104I CTCTTC 7 cut(s) 12, 513, 1189, 1269, 1426, 1594, 1912
EarI CTCTTC 7 cut(s) 12, 513, 1189, 1269, 1426, 1594, 1912
Ecl136II GAGCTC 1 cut(s) 147
Eco130I CCWWGG 6 cut(s) 1334, 1417, 1732, 1912, 2303, 2740
Eco147I AGGCCT 2 cut(s) 499, 1066
Eco24I GRGCYC 2 cut(s) 149, 1780
Eco31I GGTCTC 1 cut(s) 1823
Eco47I GGWCC 8 cut(s) 561, 740, 1227, 1676, 1718, 1838, 1964, 2030
Eco53kI GAGCTC 1 cut(s) 147
Eco81I CCTNAGG 1 cut(s) 2706
Eco88I CYCGRG 1 cut(s) 1779
EcoICRI GAGCTC 1 cut(s) 147
EcoNI CCTNNNNNAGG 2 cut(s) 1713, 2052
EcoO109I RGGNCCY 2 cut(s) 740, 3140
EcoRI GAATTC 3 cut(s) 1390, 1620, 3004
EcoT14I CCWWGG 6 cut(s) 1334, 1417, 1732, 1912, 2303, 2740
EcoT22I ATGCAT 1 cut(s) 3216
EcoT38I GRGCYC 2 cut(s) 149, 1780
ErhI CCWWGG 6 cut(s) 1334, 1417, 1732, 1912, 2303, 2740
Esp3I CGTCTC 1 cut(s) 3165
FalI AAGNNNNNCTT 2 cut(s) 2465, 2497
FaqI GGGAC 6 cut(s) 739, 1007, 1714, 1793, 1814, 1986
FauI CCCGC 2 cut(s) 715, 2913
FauNDI CATATG 2 cut(s) 1504, 3270
FbaI TGATCA 1 cut(s) 1006
FblI GTMKAC 1 cut(s) 3219
Fnu4HI GCNGC 8 cut(s) 1028, 1685, 2546, 2620, 2746, 2987, 2996, 2999
FokI GGATG 7 cut(s) 341, 683, 1195, 1307, 1564, 2198, 2199
FriOI GRGCYC 2 cut(s) 149, 1780
Fsp4HI GCNGC 8 cut(s) 1028, 1685, 2546, 2620, 2746, 2987, 2996, 2999
FspBI CTAG 4 cut(s) 744, 1335, 1698, 2126
GluI GCNGC 8 cut(s) 1028, 1685, 2546, 2620, 2746, 2987, 2996, 2999
GsaI CCCAGC 1 cut(s) 966
GsuI CTGGAG 4 cut(s) 229, 1698, 2895, 3111
HapII CCGG 6 cut(s) 1613, 1985, 2094, 3029, 3057, 3225
HgaI GACGC 1 cut(s) 292
HindIII AAGCTT 1 cut(s) 152
HpaII CCGG 6 cut(s) 1613, 1985, 2094, 3029, 3057, 3225
HphI GGTGA 7 cut(s) 316, 1118, 1698, 2444, 2521, 2780, 2961
Hpy166II GTNNAC 5 cut(s) 1493, 1721, 2030, 2897, 3220
Hpy8I GTNNAC 5 cut(s) 1493, 1721, 2030, 2897, 3220
Hpy99I CGWCG 1 cut(s) 2619
HpyCH4III ACNGT 9 cut(s) 442, 491, 587, 1040, 1058, 2611, 2690, 2803, 3158
HpyCH4IV ACGT 1 cut(s) 2316
HpyCH4V TGCA 8 cut(s) 704, 1291, 1684, 1961, 2531, 2995, 3214, 3239
HpyF10VI GCNNNNNNNGC 5 cut(s) 160, 2167, 2204, 2992, 2995
HpyF3I CTNAG 6 cut(s) 174, 408, 423, 1825, 2162, 2706
HpySE526I ACGT 1 cut(s) 2316
Kpn2I TCCGGA 1 cut(s) 3224
Ksp22I TGATCA 1 cut(s) 1006
LmnI GCTCC 7 cut(s) 1024, 1516, 1717, 2050, 2122, 2167, 2332
Lsp1109I GCAGC 6 cut(s) 1014, 1696, 2631, 2998, 3007, 3010
LweI GCATC 3 cut(s) 1217, 1402, 2542
MabI ACCWGGT 2 cut(s) 764, 1813
MaeI CTAG 4 cut(s) 744, 1335, 1698, 2126
MaeII ACGT 1 cut(s) 2316
MaeIII GTNAC 7 cut(s) 436, 458, 598, 1704, 2231, 2932, 2967
MfeI CAATTG 2 cut(s) 2990, 3078
MflI RGATCY 2 cut(s) 1167, 2296
MhlI GDGCHC 3 cut(s) 149, 1780, 2329
MlsI TGGCCA 2 cut(s) 1473, 1911
MluNI TGGCCA 2 cut(s) 1473, 1911
MlyI GAGTC 4 cut(s) 236, 974, 1400, 3266
MmeI TCCRAC 4 cut(s) 683, 1236, 1901, 3066
Mox20I TGGCCA 2 cut(s) 1473, 1911
Mph1103I ATGCAT 1 cut(s) 3216
MroI TCCGGA 1 cut(s) 3224
MroXI GAANNNNTTC 3 cut(s) 1540, 2316, 2955
MscI TGGCCA 2 cut(s) 1473, 1911
MseI TTAA 9 cut(s) 552, 834, 840, 1371, 1899, 1953, 2382, 2832, 3162
MslI CAYNNNNRTG 2 cut(s) 1041, 2291
Msp20I TGGCCA 2 cut(s) 1473, 1911
MspCI CTTAAG 1 cut(s) 2381
MspI CCGG 6 cut(s) 1613, 1985, 2094, 3029, 3057, 3225
MunI CAATTG 2 cut(s) 2990, 3078
Mva1269I GAATGC 3 cut(s) 250, 706, 1668
MwoI GCNNNNNNNGC 5 cut(s) 160, 2167, 2204, 2992, 2995
NcoI CCATGG 3 cut(s) 1417, 1732, 1912
NdeI CATATG 2 cut(s) 1504, 3270
NmeAIII GCCGAG 1 cut(s) 2698
NmuCI GTSAC 7 cut(s) 436, 458, 598, 1704, 2231, 2932, 2967
NsiI ATGCAT 1 cut(s) 3216
NspI RCATGY 2 cut(s) 1705, 3218
NspV TTCGAA 1 cut(s) 115
OliI CACNNNNGTG 1 cut(s) 1041
PaqCI CACCTGC 1 cut(s) 1717
PceI AGGCCT 2 cut(s) 499, 1066
PcsI WCGNNNNNNNCGW 2 cut(s) 1039, 2694
PctI GAATGC 3 cut(s) 250, 706, 1668
PdmI GAANNNNTTC 3 cut(s) 1540, 2316, 2955
PfeI GAWTC 9 cut(s) 170, 332, 380, 468, 482, 806, 1202, 1343, 2377
Pfl23II CGTACG 1 cut(s) 2685
PflFI GACNNNGTC 1 cut(s) 1038
PflMI CCANNNNNTGG 1 cut(s) 1673
PfoI TCCNGGA 1 cut(s) 1834
PinAI ACCGGT 2 cut(s) 1612, 2093
PkrI GCNGC 8 cut(s) 1029, 1686, 2547, 2621, 2747, 2988, 2997, 3000
PleI GAGTC 4 cut(s) 236, 974, 1400, 3265
PpsI GAGTC 4 cut(s) 236, 974, 1400, 3265
PpuMI RGGWCCY 1 cut(s) 740
PshAI GACNNNNGTC 1 cut(s) 738
Psp124BI GAGCTC 1 cut(s) 149
Psp5II RGGWCCY 1 cut(s) 740
PspFI CCCAGC 1 cut(s) 962
PspLI CGTACG 1 cut(s) 2685
PspPPI RGGWCCY 1 cut(s) 740
PstI CTGCAG 2 cut(s) 1293, 1963
PsuI RGATCY 2 cut(s) 1167, 2296
PsyI GACNNNGTC 1 cut(s) 1038
RsaI GTAC 5 cut(s) 760, 1465, 1873, 2687, 2717
RsaNI GTAC 5 cut(s) 759, 1464, 1872, 2686, 2716
RseI CAYNNNNRTG 2 cut(s) 1041, 2291
SacI GAGCTC 1 cut(s) 149
SaqAI TTAA 9 cut(s) 552, 834, 840, 1371, 1899, 1953, 2382, 2832, 3162
SatI GCNGC 8 cut(s) 1028, 1685, 2546, 2620, 2746, 2987, 2996, 2999
SchI GAGTC 4 cut(s) 236, 974, 1400, 3266
SduI GDGCHC 3 cut(s) 149, 1780, 2329
SexAI ACCWGGT 2 cut(s) 764, 1813
SfaNI GCATC 3 cut(s) 1217, 1402, 2542
SfcI CTRYAG 2 cut(s) 1289, 1959
SfuI TTCGAA 1 cut(s) 115
SinI GGWCC 8 cut(s) 561, 740, 1227, 1676, 1718, 1838, 1964, 2030
SmiMI CAYNNNNRTG 2 cut(s) 1041, 2291
SmlI CTYRAG 4 cut(s) 313, 2381, 2447, 2756
SmoI CTYRAG 4 cut(s) 313, 2381, 2447, 2756
SseBI AGGCCT 2 cut(s) 499, 1066
SsiI CCGC 5 cut(s) 722, 1514, 2545, 2746, 2906
SspMI CTAG 4 cut(s) 744, 1335, 1698, 2126
SstI GAGCTC 1 cut(s) 149
StuI AGGCCT 2 cut(s) 499, 1066
StyI CCWWGG 6 cut(s) 1334, 1417, 1732, 1912, 2303, 2740
TaaI ACNGT 9 cut(s) 442, 491, 587, 1040, 1058, 2611, 2690, 2803, 3158
TaiI ACGT 1 cut(s) 2319
TaqI TCGA 4 cut(s) 115, 880, 2088, 2825
TaqII GACCGA 4 cut(s) 578, 1065, 1244, 2775
TatI WGTACW 1 cut(s) 2715
TauI GCSGC 2 cut(s) 2548, 2748
TfiI GAWTC 9 cut(s) 170, 332, 380, 468, 482, 806, 1202, 1343, 2377
Tru1I TTAA 9 cut(s) 552, 834, 840, 1371, 1899, 1953, 2382, 2832, 3162
Tru9I TTAA 9 cut(s) 552, 834, 840, 1371, 1899, 1953, 2382, 2832, 3162
TscAI CASTG 8 cut(s) 445, 463, 496, 576, 592, 1063, 2614, 2629
TseFI GTSAC 7 cut(s) 436, 458, 598, 1704, 2231, 2932, 2967
TseI GCWGC 6 cut(s) 1027, 1684, 2619, 2986, 2995, 2998
Tsp45I GTSAC 7 cut(s) 436, 458, 598, 1704, 2231, 2932, 2967
TspGWI ACGGA 3 cut(s) 461, 859, 1206
TspRI CASTG 8 cut(s) 445, 463, 496, 576, 592, 1063, 2614, 2629
Tth111I GACNNNGTC 1 cut(s) 1038
Van91I CCANNNNNTGG 1 cut(s) 1673
Vha464I CTTAAG 1 cut(s) 2381
VpaK11BI GGWCC 8 cut(s) 561, 740, 1227, 1676, 1718, 1838, 1964, 2030
XagI CCTNNNNNAGG 2 cut(s) 1713, 2052
XceI RCATGY 2 cut(s) 1705, 3218
XcmI CCANNNNNNNNNTGG 1 cut(s) 1910
XmaJI CCTAGG 1 cut(s) 1334
XmiI GTMKAC 1 cut(s) 3219
XmnI GAANNNNTTC 3 cut(s) 1540, 2316, 2955
XspI CTAG 4 cut(s) 744, 1335, 1698, 2126
Zsp2I ATGCAT 1 cut(s) 3216
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.