Rroxscaffold_2G00127270

Belongs to the MT-A70-like family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0005856
Physical Location & Seq
Forward (+)
62743175 .. 62748058
4884 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00127270.1

Sequence Viewer

Length: 3153 bp
ATGGATTCACCTGAACGTGGTCGGAGTTATGTGAAACGTGATGTGGAGGATGGTTCTGATTTGAAGAGTGATAGGGCGGGAGATGATGAAGAGTGGGAGGGCAGTGATAAGAGGAAGCATCGTTCGAGCAGATCAAGGAAATTGGGTAATGGAGAAGAGGCGGGAGGACTAGATGGCAGTGGAAGAAGAAGAAGTCATGGGGATAGAAGTGAGAGCCGTAAACGATCAGGTGGCTCTAGTAATGCAGATAGTGATGAAGAGGACTATGACTCCAGAAAAGAGTCGCGTTCTAAGCTCATGAAGAAGAAACAAGAAGAGAGTAGTTTGGAGAAGTTGAGCAGCTGGTATCAGGATGGAGAATTGGAGAATAGGCAAGATGGTGGAGATAATAAGAGCAAAGAAGAAAGGTCTCATGATGGAGAACATGAAAAGACACTGGACAGAGATTCCAAGTATGCTGACAGGAAAGAAAGCATTCGAGAGAAGACTCATGGATCTTCCGAACAGGTGAGGACTTCGAGAAGAAAATGGGATGAATCAGATGGTGGCAAGAAAGCAGAAGAAAGTTATCATGAAAGATCTGATTCAAGAAGTAGTAAGCCTTCTGATCCTAAGTATGAGACTTCTAAAGAGAAAACTGTGTCAGCAAGAAATGAACCAAGCGAAAGTAAAATCAGGGCAATAGATTCAAGCAGTGAAAGGGGTACTAAATCTAATAACAAGGAAGAGAGAAAAGCTGATGCGGAAAAGAGTAAGAGCAAAAGCAGGGGAGAAATACTTGAAGAAGATAACAGGGGCAGTCCTATCACTCGTGAAGACAGATCAGGCAAGGAGAAAGCTGAAAAGCATAGACAGCAGAGAACTCCCACTGCTCGCGATGCTGCTGAGGGTAGGGAGAGGTCGTCTAATGCAGATGAAGATGCAAGTGCAGGGATTAAAGATAAGGGTGCTAGAGAATTTGGAAATACTACCAGGTCAAGGACACCTGAGAGGCCCGGGAGGCGATATCAGGATTCAGAACACTTTGAGATGGATTATGACCGAAGTTTTAATCTCAAGCGGAAAGAACTGGAAAAAGATGGTTATAGGGACGACCGACCTAAAGGCAGAGATGATAATTATAGTGACAGGAATAGGGAGCGGGAAGTCCCCAAAAGAGAACTGGAAAAGACGGCAAACCCCCCAGTAATGACAAGGATTCCAAAAATGGGGATATTAATTATGATCATAGCAGGGAGTGGGAGCTGCCAAGACACGGTCGTGAGAGGGGTGACAACGAAAGACCTCATGGTCGGTCTGATCCAAACTAAGCCAATAGATTATGGAAGAGCAGAGTTGGGATCCAACTTTTCTAGGAGAAACGAAGTTGGTCAACAATCCGATGGAAAATCAGCATCAAATGATGAAGAATGGACAAGGAAGAGTGATATGTATGGGTCTGGACCACCTAGGGAAGATTCAAAGGAAAGATATACTGATGATACTACCTCCTTGCGAGACCAGAGTTCATGGAAGGATGAGTTTGACTCCCATGGAGTGAAGGGAAGGGGACAAAGAGGCTCTATGCCTGGCCGCAGTGCTGGTGGCCAGAGTTCTAGTGGTGGCTCACAGCCTCCATATGGAAATGCAGAGCCAGGACCCTTCAATAGAAATGCTTCTCAAGGAGTGAAAGGGGGCCACTTACCCCCTAGCATGTCACCAGCTCCAGGTCCGCCAATGTTTCCATTTTCTCCACCTGTGTGGGCTGGGGCTCGAGGCGTTGACATCACCAATCTGGCCCTGGAAGAGGAGGTCCTCCGAGCATATCTGGCCCTGGTTTTAATTCTGCAGGGCCAATGGGGACGAGGAACTCCTGCTGATAAAAATCAAGGTGGTTGGGTTCCTCATAAAAGTAGTGGACCTCCTGGTAAAGCTCCTTCTAGAGGAGAGCAGAACGATTACTCTCAAAATTTTGTTGATACTGGTATGCGGCCTCAGAATTTCATCAGGGAGCTAGAGCTTACAAATGTTGTGGAGGATTACCCCAAGCTTAGGGAGCTTATACAGAAAAAAGATGAGATTGTGGCAAAATCTGCTTCTCATCCCATGTACCATAAATGTGACCTGAAAGAGTTTGAGCTGTCTCCGGAGTTCTTTGGAACCAAGTTTGATGTGATTCTTGTTGATCCACCATGGGAGGAATACGTTCATCGTGCTCCTGGTGTTGCTGACCATACAGAGTATTGGACATTTGAAGAAATAATGAACCTTAAGATTGAGGCAATAGCAGACACACCTTCCTTTATTTTTCTTTGGGTGGGTGATGGCATGGGTCTTGAGCAGGGCCGTCAATGTCTGAAAAAGTGGGGATTTCGTAGGTGTGAAGATATATGTTGGGTGAAGACGAACAAAACTAATGCAACTCCTGGTTTGCGGCATGATTCTCATACATTGTTTCAGCACTCAAAGGAACATTGCTTGATGGGTATAAAAGGCACCGTTCGTCGCAGCACTGATGGCCATATAATCCATGCCAACATTGATACCGATGTAATAATAGCTGAGGAACCCCCATATGGTTCAACCCAGAAGCCTGAGGACATGTACAGGATAATTGAGCATTTTGCCCTTGGTCGCAGGAGGCTTGAGCTCTTTGGTGAAGACCACAATATTCGGGCTGGTTGGCTGACTGCTGGTAAAGGATTATCCTCATCAAACTTTAATACCGAGGCTTATCTCCGGAACTTTGCTGACAAGGATGGCAAAGTTTGGCAAGGAGGGGGTGGACGAAATCCACCTCCAGAGGCACCTCATCTGGTTGTAACAACCCCTGATATAGAAGCCCTTCGGCCCAAGTCACCAATGAAAAACCAGCAGCAGATGCAGCACCAGCAATCAACATCTATTTCTCTGACAACAGCAAATTCCTCCAACAGAAGGCCTGCTGGAAACTCACCACAAAATCCAAGCGCTCTAAGTATGAACCAAGAATCTTCAAGTTCCAATCCCTCAACTCCCGCTCCTTGGGCTTCTTCGCCAATGGAGGGTTTTAAAGGGAGAGAAGGCAGCATTATGCCTTCAGATGATAAGGTTTTTGATATGTATGGTTACAGCGGACAGGCTAATGGTGACTATATAGATTTTGAGTCTCAGAGACACATGAATTTGTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MT-A70

Protein Analysis

1050

Amino Acids

117.87

Weight (kDa)

6.49

Isoelectric Point (pI)

58.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MT-A70 PF05063 716 - 893 1.2e-51 MT-A70
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1289
AccB1I GGYRCC 2 cut(s) 2474, 2785
AccBSI CCGCTC 2 cut(s) 1141, 2999
AccII CGCG 2 cut(s) 286, 876
AccIII TCCGGA 2 cut(s) 2125, 2718
AclWI GGATC 6 cut(s) 502, 602, 1294, 1335, 1348, 2159
AcoI YGGCCR 3 cut(s) 1570, 1585, 2497
AcsI RAATTY 5 cut(s) 956, 1948, 1978, 2902, 3142
AcuI CTGAAG 1 cut(s) 3042
AfaI GTAC 3 cut(s) 706, 2090, 2585
AfeI AGCGCT 1 cut(s) 2950
AflII CTTAAG 1 cut(s) 2249
AflIII ACRYGT 1 cut(s) 2580
AgsI TTSAA 9 cut(s) 64, 588, 690, 782, 1461, 1645, 2234, 2562, 2976
AjnI CCWGG 9 cut(s) 971, 1567, 1633, 1705, 1779, 1812, 1903, 2197, 2404
AleI CACNNNNGTG 2 cut(s) 1259, 1738
AloI GAACNNNNNNTCC 2 cut(s) 106, 138
Alw21I GWGCWC 2 cut(s) 2197, 2631
Alw26I GTCTC 6 cut(s) 414, 614, 1491, 2127, 3127, 3132
AlwI GGATC 6 cut(s) 502, 602, 1294, 1335, 1348, 2159
Ama87I CYCGRG 2 cut(s) 995, 1752
Aor13HI TCCGGA 2 cut(s) 2125, 2718
Aor51HI AGCGCT 1 cut(s) 2950
ApeKI GCWGC 7 cut(s) 339, 881, 1245, 2487, 2854, 2863, 3045
ApoI RAATTY 5 cut(s) 956, 1948, 1978, 2902, 3142
AseI ATTAAT 1 cut(s) 1217
Asp700I GAANNNNTTC 4 cut(s) 474, 1654, 2184, 2823
AspA2I CCTAGG 1 cut(s) 1448
AspLEI GCGC 1 cut(s) 2951
AsuC2I CCSGG 2 cut(s) 996, 997
AvaI CYCGRG 2 cut(s) 995, 1752
AvaII GGWCC 5 cut(s) 1442, 1637, 1709, 1792, 1898
AvrII CCTAGG 1 cut(s) 1448
AxyI CCTNAGG 1 cut(s) 2574
BaeI ACNNNNGTAYC 2 cut(s) 2514, 2547
BalI TGGCCA 2 cut(s) 1587, 2499
BamHI GGATCC 1 cut(s) 1340
BanI GGYRCC 2 cut(s) 2474, 2785
BanII GRGCYC 2 cut(s) 1753, 2631
BarI GAAGNNNNNNTAC 4 cut(s) 586, 618, 1900, 1932
BauI CACGAG 1 cut(s) 810
BbsI GAAGAC 4 cut(s) 491, 822, 2387, 2646
Bbv12I GWGCWC 2 cut(s) 2197, 2631
BbvCI CCTCAGC 2 cut(s) 885, 2541
BbvI GCAGC 7 cut(s) 351, 868, 1232, 2499, 2866, 2875, 3057
BceAI ACGGC 3 cut(s) 201, 1188, 2310
BciT130I CCWGG 9 cut(s) 973, 1569, 1635, 1707, 1781, 1814, 1905, 2199, 2406
BclI TGATCA 1 cut(s) 1224
BcnI CCSGG 2 cut(s) 996, 997
BcoDI GTCTC 6 cut(s) 414, 614, 1491, 2127, 3127, 3132
BfaI CTAG 9 cut(s) 170, 237, 951, 1353, 1449, 1596, 1689, 1920, 1994
BfmI CTRYAG 1 cut(s) 1826
BfoI RGCGCY 1 cut(s) 2952
BfrI CTTAAG 1 cut(s) 2249
BglI GCCNNNNNGGC 1 cut(s) 1000
BglII AGATCT 1 cut(s) 578
BlnI CCTAGG 1 cut(s) 1448
Bme18I GGWCC 5 cut(s) 1442, 1637, 1709, 1792, 1898
BmeT110I CYCGRG 2 cut(s) 995, 1752
BmiI GGNNCC 8 cut(s) 1342, 1639, 1676, 1881, 2140, 2476, 2547, 2787
BmrI ACTGGG 1 cut(s) 1178
BmsI GCATC 6 cut(s) 127, 730, 868, 910, 1403, 2850
BmuI ACTGGG 1 cut(s) 1178
BpiI GAAGAC 4 cut(s) 491, 822, 2387, 2646
BplI GAGNNNNNCTC 2 cut(s) 1511, 1543
BpmI CTGGAG 3 cut(s) 256, 1689, 2763
Bpu10I CCTNAGC 3 cut(s) 885, 2030, 2541
BpuEI CTTGAG 4 cut(s) 1040, 1644, 2336, 2645
BpuMI CCSGG 2 cut(s) 996, 997
BsaBI GATNNNNATC 1 cut(s) 1863
BsaI GGTCTC 2 cut(s) 414, 1491
BsaJI CCNNGG 9 cut(s) 995, 1448, 1531, 1779, 1812, 2171, 2608, 2706, 3002
BsaWI WCCGGW 2 cut(s) 2125, 2718
BsaXI ACNNNNNCTCC 6 cut(s) 254, 284, 887, 917, 2983, 3013
Bse1I ACTGG 5 cut(s) 441, 1074, 1167, 1184, 1966
Bse21I CCTNAGG 1 cut(s) 2574
Bse3DI GCAATG 1 cut(s) 2452
Bse8I GATNNNNATC 1 cut(s) 1863
BseAI TCCGGA 2 cut(s) 2125, 2718
BseBI CCWGG 9 cut(s) 973, 1569, 1635, 1707, 1781, 1814, 1905, 2199, 2406
BseDI CCNNGG 9 cut(s) 995, 1448, 1531, 1779, 1812, 2171, 2608, 2706, 3002
BseGI GGATG 6 cut(s) 55, 358, 538, 1522, 2080, 2743
BseJI GATNNNNATC 1 cut(s) 1863
BseMI GCAATG 1 cut(s) 2452
BseMII CTCAG 6 cut(s) 876, 978, 1988, 2532, 2565, 3143
BseNI ACTGG 5 cut(s) 441, 1074, 1167, 1184, 1966
BseRI GAGGAG 2 cut(s) 1802, 1938
BseXI GCAGC 7 cut(s) 351, 868, 1232, 2499, 2866, 2875, 3057
BseYI CCCAGC 1 cut(s) 1745
BsgI GTGCAG 1 cut(s) 948
Bsh1236I CGCG 2 cut(s) 286, 876
Bsh1285I CGRYCG 2 cut(s) 1096, 1260
BshNI GGYRCC 2 cut(s) 2474, 2785
BsiEI CGRYCG 2 cut(s) 1096, 1260
BsiHKAI GWGCWC 2 cut(s) 2197, 2631
BsiHKCI CYCGRG 2 cut(s) 995, 1752
BsiSI CCGG 3 cut(s) 996, 2126, 2719
BslFI GGGAC 4 cut(s) 1103, 1133, 1563, 1854
BsmAI GTCTC 6 cut(s) 414, 614, 1491, 2127, 3127, 3132
BsmFI GGGAC 4 cut(s) 1103, 1133, 1563, 1854
BsmI GAATGC 1 cut(s) 474
Bso31I GGTCTC 2 cut(s) 414, 1491
BsoBI CYCGRG 2 cut(s) 995, 1752
Bsp1286I GDGCHC 3 cut(s) 1753, 2197, 2631
Bsp13I TCCGGA 2 cut(s) 2125, 2718
Bsp1407I TGTACA 1 cut(s) 2583
Bsp19I CCATGG 2 cut(s) 1531, 2171
Bsp68I TCGCGA 1 cut(s) 876
BspCNI CTCAG 6 cut(s) 877, 979, 1987, 2533, 2566, 3142
BspEI TCCGGA 2 cut(s) 2125, 2718
BspFNI CGCG 2 cut(s) 286, 876
BspHI TCATGA 3 cut(s) 297, 412, 571
BspLI GGNNCC 8 cut(s) 1342, 1639, 1676, 1881, 2140, 2476, 2547, 2787
BspMAI CTGCAG 1 cut(s) 1830
BspPI GGATC 6 cut(s) 502, 602, 1294, 1335, 1348, 2159
BspQI GCTCTTC 1 cut(s) 1321
BspT107I GGYRCC 2 cut(s) 2474, 2785
BspTI CTTAAG 1 cut(s) 2249
BspTNI GGTCTC 2 cut(s) 414, 1491
BsrBI CCGCTC 2 cut(s) 1141, 2999
BsrDI GCAATG 1 cut(s) 2452
BsrGI TGTACA 1 cut(s) 2583
BsrI ACTGG 5 cut(s) 441, 1074, 1167, 1184, 1966
BssECI CCNNGG 9 cut(s) 995, 1448, 1531, 1779, 1812, 2171, 2608, 2706, 3002
BssSI CACGAG 1 cut(s) 810
BssT1I CCWWGG 5 cut(s) 1448, 1531, 2171, 2608, 3002
Bst2BI CACGAG 1 cut(s) 810
Bst2UI CCWGG 9 cut(s) 973, 1569, 1635, 1707, 1781, 1814, 1905, 2199, 2406
Bst4CI ACNGT 3 cut(s) 640, 1258, 2479
Bst6I CTCTTC 9 cut(s) 59, 84, 150, 252, 309, 720, 1321, 1415, 1779
BstAFI CTTAAG 1 cut(s) 2249
BstAPI GCANNNNNTGC 2 cut(s) 2072, 2860
BstAUI TGTACA 1 cut(s) 2583
BstC8I GCNNGC 2 cut(s) 874, 2922
BstDSI CCRYGG 2 cut(s) 1531, 2171
BstENI CCTNNNNNAGG 2 cut(s) 1784, 1920
BstF5I GGATG 6 cut(s) 55, 358, 538, 1522, 2080, 2743
BstFNI CGCG 2 cut(s) 286, 876
BstH2I RGCGCY 1 cut(s) 2952
BstHHI GCGC 1 cut(s) 2951
BstMAI GTCTC 6 cut(s) 414, 614, 1491, 2127, 3127, 3132
BstMCI CGRYCG 2 cut(s) 1096, 1260
BstNI CCWGG 9 cut(s) 973, 1569, 1635, 1707, 1781, 1814, 1905, 2199, 2406
BstNSI RCATGY 2 cut(s) 1696, 2584
BstSFI CTRYAG 1 cut(s) 1826
BstUI CGCG 2 cut(s) 286, 876
BstV1I GCAGC 7 cut(s) 351, 868, 1232, 2499, 2866, 2875, 3057
BstV2I GAAGAC 4 cut(s) 491, 822, 2387, 2646
BstX2I RGATCY 3 cut(s) 494, 578, 1340
BstXI CCANNNNNNTGG 1 cut(s) 1740
BstYI RGATCY 3 cut(s) 494, 578, 1340
Bsu36I CCTNAGG 1 cut(s) 2574
BtgI CCRYGG 2 cut(s) 1531, 2171
BtgZI GCGATG 1 cut(s) 891
BtsCI GGATG 6 cut(s) 55, 358, 538, 1522, 2080, 2743
BtsI GCAGTG 5 cut(s) 109, 184, 700, 867, 1582
BtsIMutI CAGTG 7 cut(s) 109, 184, 434, 700, 867, 1582, 2490
BtuMI TCGCGA 1 cut(s) 876
Cac8I GCNNGC 2 cut(s) 874, 2922
CciI TCATGA 3 cut(s) 297, 412, 571
CfoI GCGC 1 cut(s) 2951
Cfr9I CCCGGG 1 cut(s) 995
CsiI ACCWGGT 1 cut(s) 971
Csp6I GTAC 3 cut(s) 705, 2089, 2584
CspCI CAANNNNNGTGG 2 cut(s) 1992, 2027
CviQI GTAC 3 cut(s) 705, 2089, 2584
DraI TTTAAA 1 cut(s) 3031
DrdI GACNNNNNNGTC 1 cut(s) 1289
DseDI GACNNNNNNGTC 1 cut(s) 1289
EaeI YGGCCR 3 cut(s) 1570, 1585, 2497
Eam1104I CTCTTC 9 cut(s) 59, 84, 150, 252, 309, 720, 1321, 1415, 1779
EarI CTCTTC 9 cut(s) 59, 84, 150, 252, 309, 720, 1321, 1415, 1779
EciI GGCGGA 1 cut(s) 1701
Ecl136II GAGCTC 1 cut(s) 2629
Eco130I CCWWGG 5 cut(s) 1448, 1531, 2171, 2608, 3002
Eco147I AGGCCT 1 cut(s) 2920
Eco24I GRGCYC 2 cut(s) 1753, 2631
Eco31I GGTCTC 2 cut(s) 414, 1491
Eco32I GATATC 1 cut(s) 1007
Eco47I GGWCC 5 cut(s) 1442, 1637, 1709, 1792, 1898
Eco47III AGCGCT 1 cut(s) 2950
Eco53kI GAGCTC 1 cut(s) 2629
Eco57I CTGAAG 1 cut(s) 3042
Eco81I CCTNAGG 1 cut(s) 2574
Eco88I CYCGRG 2 cut(s) 995, 1752
EcoICRI GAGCTC 1 cut(s) 2629
EcoNI CCTNNNNNAGG 2 cut(s) 1784, 1920
EcoO109I RGGNCCY 2 cut(s) 1637, 1792
EcoRII CCWGG 9 cut(s) 971, 1567, 1633, 1705, 1779, 1812, 1903, 2197, 2404
EcoRV GATATC 1 cut(s) 1007
EcoT14I CCWWGG 5 cut(s) 1448, 1531, 2171, 2608, 3002
EcoT38I GRGCYC 2 cut(s) 1753, 2631
ErhI CCWWGG 5 cut(s) 1448, 1531, 2171, 2608, 3002
FaqI GGGAC 4 cut(s) 1103, 1133, 1563, 1854
FauI CCCGC 4 cut(s) 70, 154, 1134, 3004
FauNDI CATATG 2 cut(s) 1618, 2554
FbaI TGATCA 1 cut(s) 1224
FokI GGATG 6 cut(s) 62, 365, 545, 1529, 2067, 2750
FriOI GRGCYC 2 cut(s) 1753, 2631
FspBI CTAG 9 cut(s) 170, 237, 951, 1353, 1449, 1596, 1689, 1920, 1994
GlaI GCGC 1 cut(s) 2950
GsaI CCCAGC 1 cut(s) 1749
GsuI CTGGAG 3 cut(s) 256, 1689, 2763
HaeII RGCGCY 1 cut(s) 2952
HapII CCGG 3 cut(s) 996, 2126, 2719
HhaI GCGC 1 cut(s) 2951
Hin6I GCGC 1 cut(s) 2949
HinP1I GCGC 1 cut(s) 2949
HincII GTYRAC 2 cut(s) 1373, 1762
HindII GTYRAC 2 cut(s) 1373, 1762
HindIII AAGCTT 1 cut(s) 2027
HpaII CCGG 3 cut(s) 996, 2126, 2719
Hpy166II GTNNAC 5 cut(s) 221, 1373, 1762, 1898, 2765
Hpy8I GTNNAC 5 cut(s) 221, 1373, 1762, 1898, 2765
Hpy99I CGWCG 1 cut(s) 2487
HpyCH4III ACNGT 3 cut(s) 640, 1258, 2479
HpyCH4IV ACGT 3 cut(s) 16, 37, 2184
HpyCH4V TGCA 8 cut(s) 245, 911, 923, 929, 1628, 1828, 2399, 2863
HpySE526I ACGT 3 cut(s) 16, 37, 2184
HspAI GCGC 1 cut(s) 2949
Kpn2I TCCGGA 2 cut(s) 2125, 2718
Ksp22I TGATCA 1 cut(s) 1224
LguI GCTCTTC 1 cut(s) 1321
LmnI GCTCC 8 cut(s) 1138, 1242, 1708, 1918, 1990, 2035, 2200, 3004
Lsp1109I GCAGC 7 cut(s) 351, 868, 1232, 2499, 2866, 2875, 3057
LweI GCATC 6 cut(s) 127, 730, 868, 910, 1403, 2850
MabI ACCWGGT 1 cut(s) 971
MaeI CTAG 9 cut(s) 170, 237, 951, 1353, 1449, 1596, 1689, 1920, 1994
MaeII ACGT 3 cut(s) 16, 37, 2184
MaeIII GTNAC 8 cut(s) 1124, 1270, 1695, 2099, 2800, 2835, 3086, 3107
MbiI CCGCTC 2 cut(s) 1141, 2999
MflI RGATCY 3 cut(s) 494, 578, 1340
MhlI GDGCHC 3 cut(s) 1753, 2197, 2631
MlsI TGGCCA 2 cut(s) 1587, 2499
MluNI TGGCCA 2 cut(s) 1587, 2499
MlyI GAGTC 5 cut(s) 263, 290, 481, 1520, 3134
MmeI TCCRAC 2 cut(s) 1368, 2934
Mox20I TGGCCA 2 cut(s) 1587, 2499
MroI TCCGGA 2 cut(s) 2125, 2718
MroXI GAANNNNTTC 4 cut(s) 474, 1654, 2184, 2823
MscI TGGCCA 2 cut(s) 1587, 2499
MseI TTAA 7 cut(s) 936, 1050, 1217, 1820, 2250, 2700, 3030
MslI CAYNNNNRTG 3 cut(s) 1259, 1738, 2097
Msp20I TGGCCA 2 cut(s) 1587, 2499
MspA1I CMGCKG 2 cut(s) 342, 3093
MspCI CTTAAG 1 cut(s) 2249
MspI CCGG 3 cut(s) 996, 2126, 2719
Mva1269I GAATGC 1 cut(s) 474
MvaI CCWGG 9 cut(s) 973, 1569, 1635, 1707, 1781, 1814, 1905, 2199, 2406
MvnI CGCG 2 cut(s) 286, 876
NciI CCSGG 2 cut(s) 996, 997
NcoI CCATGG 2 cut(s) 1531, 2171
NdeI CATATG 2 cut(s) 1618, 2554
NlaIV GGNNCC 8 cut(s) 1342, 1639, 1676, 1881, 2140, 2476, 2547, 2787
NmuCI GTSAC 6 cut(s) 1124, 1270, 1695, 2099, 2835, 3107
NruI TCGCGA 1 cut(s) 876
NspI RCATGY 2 cut(s) 1696, 2584
OliI CACNNNNGTG 2 cut(s) 1259, 1738
PaeR7I CTCGAG 1 cut(s) 1752
PagI TCATGA 3 cut(s) 297, 412, 571
PceI AGGCCT 1 cut(s) 2920
PciI ACATGT 1 cut(s) 2580
PciSI GCTCTTC 1 cut(s) 1321
PctI GAATGC 1 cut(s) 474
PdmI GAANNNNTTC 4 cut(s) 474, 1654, 2184, 2823
PflFI GACNNNGTC 1 cut(s) 1256
PleI GAGTC 5 cut(s) 263, 289, 481, 1520, 3133
PpsI GAGTC 5 cut(s) 263, 289, 481, 1520, 3133
PpuMI RGGWCCY 2 cut(s) 1637, 1792
PscI ACATGT 1 cut(s) 2580
PshBI ATTAAT 1 cut(s) 1217
Psp124BI GAGCTC 1 cut(s) 2631
Psp5II RGGWCCY 2 cut(s) 1637, 1792
Psp6I CCWGG 9 cut(s) 971, 1567, 1633, 1705, 1779, 1812, 1903, 2197, 2404
PspFI CCCAGC 1 cut(s) 1745
PspGI CCWGG 9 cut(s) 971, 1567, 1633, 1705, 1779, 1812, 1903, 2197, 2404
PspN4I GGNNCC 8 cut(s) 1342, 1639, 1676, 1881, 2140, 2476, 2547, 2787
PspPPI RGGWCCY 2 cut(s) 1637, 1792
PspXI VCTCGAGB 1 cut(s) 1752
PstI CTGCAG 1 cut(s) 1830
PsuI RGATCY 3 cut(s) 494, 578, 1340
PsyI GACNNNGTC 1 cut(s) 1256
PvuII CAGCTG 1 cut(s) 342
RruI TCGCGA 1 cut(s) 876
RsaI GTAC 3 cut(s) 706, 2090, 2585
RsaNI GTAC 3 cut(s) 705, 2089, 2584
RseI CAYNNNNRTG 3 cut(s) 1259, 1738, 2097
SacI GAGCTC 1 cut(s) 2631
SapI GCTCTTC 1 cut(s) 1321
SaqAI TTAA 7 cut(s) 936, 1050, 1217, 1820, 2250, 2700, 3030
SchI GAGTC 5 cut(s) 263, 290, 481, 1520, 3134
SduI GDGCHC 3 cut(s) 1753, 2197, 2631
SexAI ACCWGGT 1 cut(s) 971
SfaNI GCATC 6 cut(s) 127, 730, 868, 910, 1403, 2850
SfcI CTRYAG 1 cut(s) 1826
Sfr274I CTCGAG 1 cut(s) 1752
SinI GGWCC 5 cut(s) 1442, 1637, 1709, 1792, 1898
SlaI CTCGAG 1 cut(s) 1752
SmaI CCCGGG 1 cut(s) 997
SmiMI CAYNNNNRTG 3 cut(s) 1259, 1738, 2097
SmlI CTYRAG 6 cut(s) 1055, 1659, 1752, 2249, 2315, 2624
SmoI CTYRAG 6 cut(s) 1055, 1659, 1752, 2249, 2315, 2624
SseBI AGGCCT 1 cut(s) 2920
SspI AATATT 1 cut(s) 2650
SspMI CTAG 9 cut(s) 170, 237, 951, 1353, 1449, 1596, 1689, 1920, 1994
SstI GAGCTC 1 cut(s) 2631
StuI AGGCCT 1 cut(s) 2920
StyI CCWWGG 5 cut(s) 1448, 1531, 2171, 2608, 3002
TaaI ACNGT 3 cut(s) 640, 1258, 2479
TaiI ACGT 3 cut(s) 19, 40, 2187
TaqI TCGA 4 cut(s) 125, 478, 518, 1753
TaqII GACCGA 3 cut(s) 1056, 1110, 1283
TatI WGTACW 1 cut(s) 2583
TauI GCSGC 3 cut(s) 1575, 1972, 2416
Tru1I TTAA 7 cut(s) 936, 1050, 1217, 1820, 2250, 2700, 3030
Tru9I TTAA 7 cut(s) 936, 1050, 1217, 1820, 2250, 2700, 3030
TscAI CASTG 7 cut(s) 109, 184, 441, 700, 874, 1582, 2497
TseFI GTSAC 6 cut(s) 1124, 1270, 1695, 2099, 2835, 3107
TseI GCWGC 7 cut(s) 339, 881, 1245, 2487, 2854, 2863, 3045
Tsp45I GTSAC 6 cut(s) 1124, 1270, 1695, 2099, 2835, 3107
TspMI CCCGGG 1 cut(s) 995
TspRI CASTG 7 cut(s) 109, 184, 441, 700, 874, 1582, 2497
Tth111I GACNNNGTC 1 cut(s) 1256
Vha464I CTTAAG 1 cut(s) 2249
VpaK11BI GGWCC 5 cut(s) 1442, 1637, 1709, 1792, 1898
VspI ATTAAT 1 cut(s) 1217
XagI CCTNNNNNAGG 2 cut(s) 1784, 1920
XapI RAATTY 5 cut(s) 956, 1948, 1978, 2902, 3142
XbaI TCTAGA 1 cut(s) 1919
XceI RCATGY 2 cut(s) 1696, 2584
XcmI CCANNNNNNNNNTGG 3 cut(s) 1159, 1595, 1777
XhoI CTCGAG 1 cut(s) 1752
XmaI CCCGGG 1 cut(s) 995
XmaJI CCTAGG 1 cut(s) 1448
XmnI GAANNNNTTC 4 cut(s) 474, 1654, 2184, 2823
XspI CTAG 9 cut(s) 170, 237, 951, 1353, 1449, 1596, 1689, 1920, 1994
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.