RLG00000018264

Belongs to the MT-A70-like family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0005856
Physical Location & Seq
Reverse (-)
27714632 .. 27719531
4900 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000018264

Sequence Viewer

Length: 3549 bp
ATGGATTCACCTGAACGTAGTCGGAGTTATGTGAAACGAGATGTGGAGGATGGTTCTGATTTGAAGAGTGATAGGGCGGGAGATGATGAAGAGTGGGAGGGCAGTGATAAGAGGAAGCATCGTTCGAGCAGATCAAGGAAATCGGGTAATGGAGAAGAGGCGGGAGGACTAGATGGCAGTGGAAGAAGAAGAAGTCATGGGGATAGAAGTGAGAGCCGTAAACGATCAGGTGGCTCTAGCAATGCGGATAGTGATGAAGAAGACTATGACTCGAGAAAAGAGTCACGTTCTAAGCTGATGAAGAAGAAACAAGAAGAGAGTAGTTTGGAGAAGTTGAAGAGCTGTTATCAGGATGGAGAATTGGAGAATAGGCAAGATGGTGGAGATAAGTCAGGGAGTAGAGGGCTGATTAGAGCTGAAGAAAATGAAAGAAGGAAACTGGCTTCAAAGCTCGCACAGCACGAGAATTCCCACACTAAAAGTAAGAGCAAAGAAGAAAGGTCTCATGATGGAGAACATGAAAAGACACTGGACAGAGATTCCAAGTATGCTGACAGGAAAGAAAGCATTCGAGAGAAGACTCATGGATCTTCCGAACAGGTAAGGACTTCGAGAAGAAAATGGGATGAATCAGATGGTGGCAAGAAAGCAGAAGAAAGTTATCATGAAAGATCTGATTCAAGAAGTAGTAAGCCTTCTGATCCAAAGTATGAGACTTCTAAAGAGAAAACTGTGTCAGCAAGAAATGAACCAAGCGAAAGTAAAATCAGGGGAATAGATTCAAGCAGTGAAAGGGGTACTAAATCTAATAACAAGGAAGAGAGAAAAGCTGATGCGGAAAAGAGTAAGAGCAAAAGCAGGGGAGAAATACTTGAAGAAGATAACAGGGGCAGTCCTATCACTCGTGAAGACAGATCAGGCAAGGAGAAAGCTGAAAAGCATAGACAGCAGAGAACTCCCACTGCTCGTGATGCTGCTGAGGGTAGGGAGAGGTCGTCTAATGCTGATGAAGATGCAAGTGCAGGGATTAAAGATAAGGGTGCTAGAGAATTTGGAAATACTACCAGGTCAAGGACACCTGAGAGGCCTGGGAGGCGATATCAGGATTCAGAACACTTTGAGACGGATTATGACCGAAGTTTTAATCTCAAGCGGAAAGAACTGGAAAAAGATGGTTATAGGGACGACCGACCTAAAGGCAGAGATGATAACTATAGTGACAGGAATAGGGACCGGGAAGTCCCCAAAGAGAACTGGAAAAGACGGCAAACCCCCAGTAATGACAAGGATTCCAAAAATGGGGATATTAGTTATGATCATAGCAGGGAGTGGGAGCTGCCGAGACACGGTCGTGAGAGGGGTGACAACGAAAGACCTCATGGTCGGTCTGGTAATAGGAAAGATGGAAGCCGGGGTGAAGCTGTGAAAACTTCATCAAACTTTGGAATTTCAAATGAAAATTATGATGTGATTGAGATCCAAACTAAGCCAATAGATTATGGAAGAGCAGAGTTGGGATCCAACTTTTCTAGGAGAAACGAAGTTGGTCAACAATCCGATGGAAAATCAGCATCAAATGATGAAGAATGGACAAGGAAGAGTGATATGTATGGGTCTGGACCACCTAGGGAAGATTCAAAGGAAAGATATACTGATGATACTACCTCCTTACGAGACCAGAGTTCATGGAAGGATGAGTTTGACTCCCATGGAGTGAAGGGAAGGGGACAAAGAGGTTCTATGCCTGGCCGCAATGCTGGTGGCCAGAGTTCTAGTGGTGGTTCACAGCCTCCATATGGAAATGCAGAGCCAGGACCCTTCAATAGAAATACTTCTCAAGGAGTGAAAGGGGGTAGAGGCGGGAGAGGAGGAAGGGGTAGGCTTACTGGAAGAGATGGCCAGCAGGTAGCAATTCCAATTCCGATAATGGGATCACCATTTGGACCTATTGGAATGCCTCCGCCTGGACCCATGCAGCCACTTACCCCTAGCATGTCACCAGCTCCAGGTCCGCCAATGTTTCCATTTTCTCCACCTGTGTGGGCTGGGGCTCGAGGCGTTGACATCAGTATGTTATCCATCCCACCCACTGTTCCTCATGGATCATCAGGCCCAAGATTTCCTCCTAATATGGTGACTCCAACTAACCCTTCCATGTTTTTTAGCCAATCTGGGCCTGGAAGAGGAGGTCCTCCGAGCATATCTGGCCCTGGTTTTAATTCTGCAGCGCCAATGGGACGAGGAACTCCTGCTGATAAAAATCAAGGTGGTTGGGTTCCTCATAAAAGTAGTGGACCTCCTGGTAAAGCTCCTTCTAGAGGAGAGCAGAACGATTACTCTCAAAATTTTGTTGATACTGGTATGCGGCCCCAGAATTTCATCAGGGAGCTAGAGCTTACAAATGTTGTGGAGGATTACCCCAAGCTTAGGGAGCTTATACAGAAAAAAGATGAGATTGTGGCAAAATCTGCTTCTCATCCCATGTACCATAAATGTGATCTGAAAGAGTTTGAGCTTTCTCCGGAGTTCTTTGGAACCAAGTTTGATGTGATTCTTGTTGATCCACCATGGGAGGAATACGTTCATCGCGCTCCTGGTGTTGCTGACCATACAGAGTATTGGACATTTGAAGAAATAATGAACCTTAAGATTGAGGCAATAGCAGACACACCTTCCTTTATTTTTCTTTGGGTGGGTGATGGCATGGGTCTTGAGCAGGGCCGTCAATGTCTGAAAAAGTGGGGATTTCGTAGGTGTGAAGATATATGTTGGGTGAAGACGAACAAAACTAATGCAACTCCTGGTTTGCGCCATGATTCTCATACATTGTTTCAGCACTCAAAGGAACATTGCTTGATGGGTATAAAAGGCACCGTTCGTCGCAGCACTGATGGCCATATAATCCATGCCAACATTGATACCGATGTAATAATAGCTGAGGAACCCCCATATGGTTCAACCCAGAAGCCTGAGGACATGTACAGGATAATTGAGCATTTTGCGCTTGGTCGCAGGAGGCTTGAGCTCTTTGGTGAAGACCACAATATTCGGGCTGGTTGGCTGACTGTTGGTAAAGGATTATCCTCATCAAACTTTAATGCTGAGGCTTATCTCCGGAACTTTGCTGACAAGGATGGGAAAGTTTGGCAAGGAGGGGGTGGACGAAATCCACCTCCAGAGGCACCTCATCTGGTTGTAACGACCCCTGATATAGAAGCCCTTCGGCCCAAGTCACCAATGAAAAACCAACAGCAGATGCAGCACCAGCAATCAACATCTATTTCTCTGACAACAGCAAATTCCTCCAACAGAAGGCCTGCTGGAAACTCACCACAAAATCCAAGCGCTCTAGGTATGAACCAAGAAGCTTCAAGTTCCAATCCCTCAACTCCAGCTCCTTGGGCTTCTTCGCCAATGGAGGGTTTTAAAGGGAGAGAGGGCGGCATTATGCCTTCAGATGATAAGGTTTTTGATATGTATGGTTACAGCGGACAGGCAAATGGTGACTATATAGATTTTGAGTCTCAGAGACACATGAATTTGTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MT-A70

Protein Analysis

1183

Amino Acids

131.9

Weight (kDa)

8.3

Isoelectric Point (pI)

56.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MT-A70 PF05063 848 - 1025 3.1e-52 MT-A70
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 1238, 1380
Acc36I ACCTGC 1 cut(s) 1894
AccB1I GGYRCC 2 cut(s) 2870, 3181
AccII CGCG 1 cut(s) 2589
AccIII TCCGGA 2 cut(s) 2521, 3114
AclWI GGATC 8 cut(s) 595, 695, 1471, 1512, 1525, 1939, 2110, 2555
AcoI YGGCCR 4 cut(s) 1747, 1762, 1897, 2893
AcsI RAATTY 7 cut(s) 466, 1049, 1446, 2344, 2374, 3298, 3538
AcuI CTGAAG 2 cut(s) 438, 3438
AfaI GTAC 3 cut(s) 799, 2486, 2981
AfeI AGCGCT 1 cut(s) 3346
AflII CTTAAG 1 cut(s) 2645
AflIII ACRYGT 1 cut(s) 2976
AjuI GAANNNNNNNTTGG 2 cut(s) 2134, 2166
AleI CACNNNNGTG 2 cut(s) 1350, 2038
AloI GAACNNNNNNTCC 2 cut(s) 106, 138
Alw21I GWGCWC 1 cut(s) 3027
Alw26I GTCTC 7 cut(s) 507, 707, 1115, 1336, 1668, 3523, 3528
AlwI GGATC 8 cut(s) 595, 695, 1471, 1512, 1525, 1939, 2110, 2555
Ama87I CYCGRG 2 cut(s) 271, 2052
Aor13HI TCCGGA 2 cut(s) 2521, 3114
Aor51HI AGCGCT 1 cut(s) 3346
ApeKI GCWGC 6 cut(s) 974, 1336, 1975, 2225, 2883, 3259
ApoI RAATTY 7 cut(s) 466, 1049, 1446, 2344, 2374, 3298, 3538
Asp700I GAANNNNTTC 5 cut(s) 567, 778, 1831, 2580, 3219
AspA2I CCTAGG 1 cut(s) 1625
AspLEI GCGC 5 cut(s) 2230, 2591, 2811, 3004, 3347
AsuC2I CCSGG 2 cut(s) 1235, 1412
AvaI CYCGRG 2 cut(s) 271, 2052
AvaII GGWCC 8 cut(s) 1231, 1619, 1814, 1943, 1967, 2009, 2189, 2294
AvrII CCTAGG 1 cut(s) 1625
AxyI CCTNAGG 1 cut(s) 2970
BaeI ACNNNNGTAYC 2 cut(s) 2910, 2943
BalI TGGCCA 3 cut(s) 1764, 1899, 2895
BamHI GGATCC 1 cut(s) 1517
BanI GGYRCC 2 cut(s) 2870, 3181
BanII GRGCYC 2 cut(s) 2053, 3027
BarI GAAGNNNNNNTAC 4 cut(s) 679, 711, 2296, 2328
BauI CACGAG 3 cut(s) 461, 903, 966
BbsI GAAGAC 5 cut(s) 267, 584, 915, 2783, 3042
Bbv12I GWGCWC 1 cut(s) 3027
BbvCI CCTCAGC 3 cut(s) 978, 2937, 3102
BbvI GCAGC 6 cut(s) 961, 1323, 1987, 2237, 2895, 3271
BceAI ACGGC 3 cut(s) 201, 1280, 2706
BclI TGATCA 1 cut(s) 1315
BcnI CCSGG 2 cut(s) 1235, 1412
BcoDI GTCTC 7 cut(s) 507, 707, 1115, 1336, 1668, 3523, 3528
BfmI CTRYAG 2 cut(s) 1213, 2223
BfoI RGCGCY 2 cut(s) 2231, 3348
BfrI CTTAAG 1 cut(s) 2645
BfuAI ACCTGC 1 cut(s) 1894
BglI GCCNNNNNGGC 1 cut(s) 1093
BglII AGATCT 1 cut(s) 671
BisI GCNGC 9 cut(s) 975, 1337, 1750, 1976, 2226, 2366, 2884, 3260, 3442
BlnI CCTAGG 1 cut(s) 1625
BlsI GCNGC 9 cut(s) 976, 1338, 1751, 1977, 2227, 2367, 2885, 3261, 3443
Bme18I GGWCC 8 cut(s) 1231, 1619, 1814, 1943, 1967, 2009, 2189, 2294
BmeT110I CYCGRG 2 cut(s) 271, 2052
BmrI ACTGGG 1 cut(s) 1269
BmsI GCATC 6 cut(s) 127, 823, 961, 1003, 1580, 3246
BmuI ACTGGG 1 cut(s) 1269
BpiI GAAGAC 5 cut(s) 267, 584, 915, 2783, 3042
BplI GAGNNNNNCTC 2 cut(s) 1688, 1720
BpmI CTGGAG 3 cut(s) 1989, 3159, 3375
Bpu10I CCTNAGC 4 cut(s) 978, 2426, 2937, 3102
BpuEI CTTGAG 4 cut(s) 1133, 1821, 2732, 3041
BpuMI CCSGG 2 cut(s) 1235, 1412
BsaBI GATNNNNATC 2 cut(s) 1475, 2259
BsaI GGTCTC 2 cut(s) 507, 1668
BsaJI CCNNGG 7 cut(s) 1088, 1411, 1625, 1708, 2209, 2567, 3398
BsaWI WCCGGW 2 cut(s) 2521, 3114
BsaXI ACNNNNNCTCC 4 cut(s) 980, 1010, 3379, 3409
Bse1I ACTGG 7 cut(s) 444, 534, 1167, 1259, 1275, 1891, 2362
Bse21I CCTNAGG 1 cut(s) 2970
Bse3DI GCAATG 3 cut(s) 247, 1759, 2848
Bse8I GATNNNNATC 2 cut(s) 1475, 2259
BseAI TCCGGA 2 cut(s) 2521, 3114
BseDI CCNNGG 7 cut(s) 1088, 1411, 1625, 1708, 2209, 2567, 3398
BseGI GGATG 7 cut(s) 55, 358, 631, 1699, 2079, 2476, 3139
BseJI GATNNNNATC 2 cut(s) 1475, 2259
BseMI GCAATG 3 cut(s) 247, 1759, 2848
BseMII CTCAG 6 cut(s) 969, 1071, 2928, 2961, 3093, 3539
BseNI ACTGG 7 cut(s) 444, 534, 1167, 1259, 1275, 1891, 2362
BseRI GAGGAG 3 cut(s) 1881, 2199, 2334
BseXI GCAGC 6 cut(s) 961, 1323, 1987, 2237, 2895, 3271
BseYI CCCAGC 1 cut(s) 2045
BsgI GTGCAG 1 cut(s) 1041
Bsh1236I CGCG 1 cut(s) 2589
Bsh1285I CGRYCG 2 cut(s) 1189, 1351
BshNI GGYRCC 2 cut(s) 2870, 3181
BsiEI CGRYCG 2 cut(s) 1189, 1351
BsiHKAI GWGCWC 1 cut(s) 3027
BsiHKCI CYCGRG 2 cut(s) 271, 2052
BsiSI CCGG 4 cut(s) 1234, 1411, 2522, 3115
BslFI GGGAC 5 cut(s) 1196, 1226, 1244, 1740, 2250
BsmAI GTCTC 7 cut(s) 507, 707, 1115, 1336, 1668, 3523, 3528
BsmBI CGTCTC 1 cut(s) 1115
BsmFI GGGAC 5 cut(s) 1196, 1226, 1244, 1740, 2250
BsmI GAATGC 2 cut(s) 567, 1959
Bso31I GGTCTC 2 cut(s) 507, 1668
BsoBI CYCGRG 2 cut(s) 271, 2052
Bsp1286I GDGCHC 2 cut(s) 2053, 3027
Bsp13I TCCGGA 2 cut(s) 2521, 3114
Bsp1407I TGTACA 1 cut(s) 2979
Bsp19I CCATGG 2 cut(s) 1708, 2567
BspCNI CTCAG 6 cut(s) 970, 1072, 2929, 2962, 3094, 3538
BspEI TCCGGA 2 cut(s) 2521, 3114
BspFNI CGCG 1 cut(s) 2589
BspHI TCATGA 2 cut(s) 505, 664
BspMAI CTGCAG 1 cut(s) 2227
BspMI ACCTGC 1 cut(s) 1894
BspPI GGATC 8 cut(s) 595, 695, 1471, 1512, 1525, 1939, 2110, 2555
BspQI GCTCTTC 2 cut(s) 332, 1498
BspT107I GGYRCC 2 cut(s) 2870, 3181
BspTI CTTAAG 1 cut(s) 2645
BspTNI GGTCTC 2 cut(s) 507, 1668
BsrDI GCAATG 3 cut(s) 247, 1759, 2848
BsrGI TGTACA 1 cut(s) 2979
BsrI ACTGG 7 cut(s) 444, 534, 1167, 1259, 1275, 1891, 2362
BssECI CCNNGG 7 cut(s) 1088, 1411, 1625, 1708, 2209, 2567, 3398
BssSI CACGAG 3 cut(s) 461, 903, 966
BssT1I CCWWGG 4 cut(s) 1625, 1708, 2567, 3398
Bst2BI CACGAG 3 cut(s) 461, 903, 966
Bst4CI ACNGT 5 cut(s) 733, 1349, 2092, 2875, 3067
BstAFI CTTAAG 1 cut(s) 2645
BstAPI GCANNNNNTGC 1 cut(s) 2468
BstAUI TGTACA 1 cut(s) 2979
BstC8I GCNNGC 3 cut(s) 453, 1901, 3318
BstDEI CTNAG 9 cut(s) 291, 978, 1080, 1485, 2426, 2937, 2970, 3102, 3525
BstDSI CCRYGG 2 cut(s) 1708, 2567
BstENI CCTNNNNNAGG 2 cut(s) 2181, 2316
BstF5I GGATG 7 cut(s) 55, 358, 631, 1699, 2079, 2476, 3139
BstFNI CGCG 1 cut(s) 2589
BstH2I RGCGCY 2 cut(s) 2231, 3348
BstHHI GCGC 5 cut(s) 2230, 2591, 2811, 3004, 3347
BstMAI GTCTC 7 cut(s) 507, 707, 1115, 1336, 1668, 3523, 3528
BstMCI CGRYCG 2 cut(s) 1189, 1351
BstNSI RCATGY 2 cut(s) 1996, 2980
BstSFI CTRYAG 2 cut(s) 1213, 2223
BstUI CGCG 1 cut(s) 2589
BstV1I GCAGC 6 cut(s) 961, 1323, 1987, 2237, 2895, 3271
BstV2I GAAGAC 5 cut(s) 267, 584, 915, 2783, 3042
BstX2I RGATCY 4 cut(s) 587, 671, 1476, 1517
BstXI CCANNNNNNTGG 2 cut(s) 2040, 3399
BstYI RGATCY 4 cut(s) 587, 671, 1476, 1517
Bsu36I CCTNAGG 1 cut(s) 2970
BtgI CCRYGG 2 cut(s) 1708, 2567
BtgZI GCGATG 1 cut(s) 2570
BtsCI GGATG 7 cut(s) 55, 358, 631, 1699, 2079, 2476, 3139
BtsI GCAGTG 4 cut(s) 109, 184, 793, 960
BtsIMutI CAGTG 7 cut(s) 109, 184, 527, 793, 960, 2088, 2886
BveI ACCTGC 1 cut(s) 1894
Cac8I GCNNGC 3 cut(s) 453, 1901, 3318
CciI TCATGA 2 cut(s) 505, 664
CfoI GCGC 5 cut(s) 2230, 2591, 2811, 3004, 3347
CsiI ACCWGGT 1 cut(s) 1064
Csp6I GTAC 3 cut(s) 798, 2485, 2980
CspCI CAANNNNNGTGG 4 cut(s) 1741, 1776, 2388, 2423
CviQI GTAC 3 cut(s) 798, 2485, 2980
DdeI CTNAG 9 cut(s) 291, 978, 1080, 1485, 2426, 2937, 2970, 3102, 3525
DraI TTTAAA 1 cut(s) 3427
DrdI GACNNNNNNGTC 2 cut(s) 1238, 1380
DseDI GACNNNNNNGTC 2 cut(s) 1238, 1380
EaeI YGGCCR 4 cut(s) 1747, 1762, 1897, 2893
EciI GGCGGA 2 cut(s) 1950, 2001
Ecl136II GAGCTC 1 cut(s) 3025
Eco130I CCWWGG 4 cut(s) 1625, 1708, 2567, 3398
Eco147I AGGCCT 2 cut(s) 1087, 3316
Eco24I GRGCYC 2 cut(s) 2053, 3027
Eco31I GGTCTC 2 cut(s) 507, 1668
Eco32I GATATC 1 cut(s) 1100
Eco47I GGWCC 8 cut(s) 1231, 1619, 1814, 1943, 1967, 2009, 2189, 2294
Eco47III AGCGCT 1 cut(s) 3346
Eco53kI GAGCTC 1 cut(s) 3025
Eco57I CTGAAG 2 cut(s) 438, 3438
Eco81I CCTNAGG 1 cut(s) 2970
Eco88I CYCGRG 2 cut(s) 271, 2052
EcoICRI GAGCTC 1 cut(s) 3025
EcoNI CCTNNNNNAGG 2 cut(s) 2181, 2316
EcoO109I RGGNCCY 2 cut(s) 1814, 2189
EcoRI GAATTC 1 cut(s) 466
EcoRV GATATC 1 cut(s) 1100
EcoT14I CCWWGG 4 cut(s) 1625, 1708, 2567, 3398
EcoT38I GRGCYC 2 cut(s) 2053, 3027
ErhI CCWWGG 4 cut(s) 1625, 1708, 2567, 3398
Esp3I CGTCTC 1 cut(s) 1115
FaqI GGGAC 5 cut(s) 1196, 1226, 1244, 1740, 2250
FauI CCCGC 3 cut(s) 70, 154, 1853
FauNDI CATATG 2 cut(s) 1795, 2950
FbaI TGATCA 1 cut(s) 1315
Fnu4HI GCNGC 9 cut(s) 975, 1337, 1750, 1976, 2226, 2366, 2884, 3260, 3442
FokI GGATG 7 cut(s) 62, 365, 638, 1706, 2066, 2463, 3146
FriOI GRGCYC 2 cut(s) 2053, 3027
Fsp4HI GCNGC 9 cut(s) 975, 1337, 1750, 1976, 2226, 2366, 2884, 3260, 3442
GlaI GCGC 5 cut(s) 2229, 2590, 2810, 3003, 3346
GluI GCNGC 9 cut(s) 975, 1337, 1750, 1976, 2226, 2366, 2884, 3260, 3442
GsaI CCCAGC 1 cut(s) 2049
GsuI CTGGAG 3 cut(s) 1989, 3159, 3375
HaeII RGCGCY 2 cut(s) 2231, 3348
HapII CCGG 4 cut(s) 1234, 1411, 2522, 3115
HhaI GCGC 5 cut(s) 2230, 2591, 2811, 3004, 3347
Hin6I GCGC 5 cut(s) 2228, 2589, 2809, 3002, 3345
HinP1I GCGC 5 cut(s) 2228, 2589, 2809, 3002, 3345
HincII GTYRAC 2 cut(s) 1550, 2062
HindII GTYRAC 2 cut(s) 1550, 2062
HindIII AAGCTT 2 cut(s) 2423, 3366
HpaII CCGG 4 cut(s) 1234, 1411, 2522, 3115
Hpy166II GTNNAC 6 cut(s) 221, 1550, 1784, 2062, 2294, 3161
Hpy8I GTNNAC 6 cut(s) 221, 1550, 1784, 2062, 2294, 3161
Hpy99I CGWCG 1 cut(s) 2883
HpyCH4III ACNGT 5 cut(s) 733, 1349, 2092, 2875, 3067
HpyCH4IV ACGT 3 cut(s) 16, 286, 2580
HpyCH4V TGCA 7 cut(s) 1016, 1022, 1805, 1975, 2225, 2795, 3259
HpyF3I CTNAG 9 cut(s) 291, 978, 1080, 1485, 2426, 2937, 2970, 3102, 3525
HpySE526I ACGT 3 cut(s) 16, 286, 2580
HspAI GCGC 5 cut(s) 2228, 2589, 2809, 3002, 3345
Kpn2I TCCGGA 2 cut(s) 2521, 3114
Ksp22I TGATCA 1 cut(s) 1315
LguI GCTCTTC 2 cut(s) 332, 1498
LmnI GCTCC 7 cut(s) 1333, 2008, 2314, 2386, 2431, 2596, 3400
Lsp1109I GCAGC 6 cut(s) 961, 1323, 1987, 2237, 2895, 3271
LweI GCATC 6 cut(s) 127, 823, 961, 1003, 1580, 3246
MabI ACCWGGT 1 cut(s) 1064
MaeII ACGT 3 cut(s) 16, 286, 2580
MaeIII GTNAC 9 cut(s) 282, 1217, 1361, 1995, 2134, 3196, 3231, 3482, 3503
MflI RGATCY 4 cut(s) 587, 671, 1476, 1517
MhlI GDGCHC 2 cut(s) 2053, 3027
MlsI TGGCCA 3 cut(s) 1764, 1899, 2895
MluNI TGGCCA 3 cut(s) 1764, 1899, 2895
MlyI GAGTC 6 cut(s) 263, 290, 574, 1697, 2131, 3530
MmeI TCCRAC 3 cut(s) 1545, 2165, 3330
Mox20I TGGCCA 3 cut(s) 1764, 1899, 2895
MroI TCCGGA 2 cut(s) 2521, 3114
MroXI GAANNNNTTC 5 cut(s) 567, 778, 1831, 2580, 3219
MscI TGGCCA 3 cut(s) 1764, 1899, 2895
MseI TTAA 6 cut(s) 1029, 1143, 2217, 2646, 3096, 3426
MslI CAYNNNNRTG 4 cut(s) 1350, 2038, 2069, 2493
Msp20I TGGCCA 3 cut(s) 1764, 1899, 2895
MspA1I CMGCKG 1 cut(s) 3489
MspCI CTTAAG 1 cut(s) 2645
MspI CCGG 4 cut(s) 1234, 1411, 2522, 3115
Mva1269I GAATGC 2 cut(s) 567, 1959
MvnI CGCG 1 cut(s) 2589
NciI CCSGG 2 cut(s) 1235, 1412
NcoI CCATGG 2 cut(s) 1708, 2567
NdeI CATATG 2 cut(s) 1795, 2950
NmeAIII GCCGAG 1 cut(s) 1365
NmuCI GTSAC 7 cut(s) 282, 1217, 1361, 1995, 2134, 3231, 3503
NspI RCATGY 2 cut(s) 1996, 2980
OliI CACNNNNGTG 2 cut(s) 1350, 2038
PaeR7I CTCGAG 2 cut(s) 271, 2052
PagI TCATGA 2 cut(s) 505, 664
PceI AGGCCT 2 cut(s) 1087, 3316
PciI ACATGT 1 cut(s) 2976
PciSI GCTCTTC 2 cut(s) 332, 1498
PcsI WCGNNNNNNNCGW 1 cut(s) 459
PctI GAATGC 2 cut(s) 567, 1959
PdmI GAANNNNTTC 5 cut(s) 567, 778, 1831, 2580, 3219
PflFI GACNNNGTC 1 cut(s) 1347
PkrI GCNGC 9 cut(s) 976, 1338, 1751, 1977, 2227, 2367, 2885, 3261, 3443
PleI GAGTC 6 cut(s) 263, 289, 574, 1697, 2131, 3529
PpsI GAGTC 6 cut(s) 263, 289, 574, 1697, 2131, 3529
PpuMI RGGWCCY 2 cut(s) 1814, 2189
PscI ACATGT 1 cut(s) 2976
Psp124BI GAGCTC 1 cut(s) 3027
Psp5II RGGWCCY 2 cut(s) 1814, 2189
PspFI CCCAGC 1 cut(s) 2045
PspPPI RGGWCCY 2 cut(s) 1814, 2189
PspXI VCTCGAGB 1 cut(s) 2052
PstI CTGCAG 1 cut(s) 2227
PsuI RGATCY 4 cut(s) 587, 671, 1476, 1517
PsyI GACNNNGTC 1 cut(s) 1347
RsaI GTAC 3 cut(s) 799, 2486, 2981
RsaNI GTAC 3 cut(s) 798, 2485, 2980
RseI CAYNNNNRTG 4 cut(s) 1350, 2038, 2069, 2493
SacI GAGCTC 1 cut(s) 3027
SapI GCTCTTC 2 cut(s) 332, 1498
SaqAI TTAA 6 cut(s) 1029, 1143, 2217, 2646, 3096, 3426
SatI GCNGC 9 cut(s) 975, 1337, 1750, 1976, 2226, 2366, 2884, 3260, 3442
SchI GAGTC 6 cut(s) 263, 290, 574, 1697, 2131, 3530
SduI GDGCHC 2 cut(s) 2053, 3027
SexAI ACCWGGT 1 cut(s) 1064
SfaNI GCATC 6 cut(s) 127, 823, 961, 1003, 1580, 3246
SfcI CTRYAG 2 cut(s) 1213, 2223
Sfr274I CTCGAG 2 cut(s) 271, 2052
SinI GGWCC 8 cut(s) 1231, 1619, 1814, 1943, 1967, 2009, 2189, 2294
SlaI CTCGAG 2 cut(s) 271, 2052
SmiMI CAYNNNNRTG 4 cut(s) 1350, 2038, 2069, 2493
SmlI CTYRAG 7 cut(s) 271, 1148, 1836, 2052, 2645, 2711, 3020
SmoI CTYRAG 7 cut(s) 271, 1148, 1836, 2052, 2645, 2711, 3020
SseBI AGGCCT 2 cut(s) 1087, 3316
SspI AATATT 1 cut(s) 3046
SstI GAGCTC 1 cut(s) 3027
StuI AGGCCT 2 cut(s) 1087, 3316
StyI CCWWGG 4 cut(s) 1625, 1708, 2567, 3398
TaaI ACNGT 5 cut(s) 733, 1349, 2092, 2875, 3067
TaiI ACGT 3 cut(s) 19, 289, 2583
TaqI TCGA 5 cut(s) 125, 272, 571, 611, 2053
TaqII GACCGA 3 cut(s) 1149, 1203, 1374
TatI WGTACW 1 cut(s) 2979
TauI GCSGC 3 cut(s) 1752, 2368, 3444
Tru1I TTAA 6 cut(s) 1029, 1143, 2217, 2646, 3096, 3426
Tru9I TTAA 6 cut(s) 1029, 1143, 2217, 2646, 3096, 3426
TscAI CASTG 7 cut(s) 109, 184, 534, 793, 967, 2095, 2893
TseFI GTSAC 7 cut(s) 282, 1217, 1361, 1995, 2134, 3231, 3503
TseI GCWGC 6 cut(s) 974, 1336, 1975, 2225, 2883, 3259
Tsp45I GTSAC 7 cut(s) 282, 1217, 1361, 1995, 2134, 3231, 3503
TspGWI ACGGA 1 cut(s) 1139
TspRI CASTG 7 cut(s) 109, 184, 534, 793, 967, 2095, 2893
Tth111I GACNNNGTC 1 cut(s) 1347
Vha464I CTTAAG 1 cut(s) 2645
VpaK11BI GGWCC 8 cut(s) 1231, 1619, 1814, 1943, 1967, 2009, 2189, 2294
XagI CCTNNNNNAGG 2 cut(s) 2181, 2316
XapI RAATTY 7 cut(s) 466, 1049, 1446, 2344, 2374, 3298, 3538
XbaI TCTAGA 1 cut(s) 2315
XceI RCATGY 2 cut(s) 1996, 2980
XcmI CCANNNNNNNNNTGG 2 cut(s) 1772, 2174
XhoI CTCGAG 2 cut(s) 271, 2052
XmaJI CCTAGG 1 cut(s) 1625
XmnI GAANNNNTTC 5 cut(s) 567, 778, 1831, 2580, 3219
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.