Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr07

Orthogroup ID

OG0012917

Physical Location & Seq

Genomic Coordinates Forward (+)

34729027 .. 34732087

Gene Structure 3061 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD07G1285000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1323 bp

ATGTTGAGGGCAGAAGGTTATTCTGTTGGTTGTTATACTAGCCCGCACATTCAAACTATAAGGGAACGTATATCAGTGGGCAGGTTTGCTGAACCGGTGTCAGCGAAGGCGTTGAGTTCTCTTTTTGATAGGGTTAAGAAGATTCTGGATCATGCAATAGAAGTTGAAAATGGGCGTACAAGTCATTTTGAGGTTCTCACTGCTTTGGCATTCACATTGTTTGCTCAAGAGAATGTTGACATAGCAGTTATTGAGGCTGGGTTGGGGGGCGCACGTGATGCAACTAATGTCATTTGTAGCTCTGAACTTGCTGCATCGGTCATTACTACGATAGGTGAGGAGCATTTGGCTGCACTTGGGGGTTCTTTGGAAAGCATTGCAATGGCGAAGTCTGGAATCATTAAACATGGCTGTCCAGTGGTTCTGGGTGGTCCATTCCTCCCACATATTGAACAAATTCTTCGTGATAAAGCATTGTTGATGAGTTCCCCTGTAGTATTAGCATCTGATACTGGAAATAAAAGTAAGATAAATGGTGTTAGCATGCTCAATGGCAGACCTTTCCAATCTTGTGATATAGTCATACGAGTTGAAAGGGACTTAAAGCTGTTCATTGAGCTATTGGATGTGAAACTATACATGCTTGGAACTCACCAACTTCAGAATGCAGCAACTGCTACTTGTGCAGCACTATGTCTCCGCAACTTAGGATGGAGAATTTCAGACGGATCCATTAGAACTGGTTTAGAGCAAACAAGATTGTTTGGAAGAAGTCAGTTTCTTACATCAAAAGAAGCTCAGGCATTAGGATTATCTGGGACAACAATATTGCTTGATGGAGCCCACACCAAAGAGTCTGCTAGAGCGCTGATGGACACTGTAAAGATGACATTTCCAGAGGCAGGACTGACTCTTGTGGTTGCAATGGCTAGTGACAAAGATCATCTAGGTTTTGCAAAAGAGTGCCTGTCAGGTGGGCATGTAGAGGGTGTTGTTCTGACCGAAGCTAACATCGCTGGGGCAACATCTCGCACAACTGCAGCCTCATCGTTAAGAGATTGCTGGATCCAAGCTTCACAAGAATTGGAAACAGATATTCTTCACGACGAAATGCCTGAACACCAAAACTCAACTATGGATCAATTACTTTGCATAAGCAAGTCAGGAAATAAACCGATTTTGTCCACTGAGGGTTCGTTTCAGGCTTCCCTGAAGATAACAAATCAGATTATCAAAGGAAGAACGGGGGACCGGTTGCAAATTGTTGTAATTGCTGGTTCCTTACATATTGTTTCACTGGTGTTAGCTACCCTTCATGATTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0003674 GO:0003824 GO:0004326 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005759 GO:0006082 GO:0006575 GO:0006725 GO:0006732 GO:0006760 GO:0006761 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0008841 GO:0009058 GO:0009108 GO:0009396 GO:0009790 GO:0009791 GO:0009793 GO:0009987 GO:0010154 GO:0016053 GO:0016874 GO:0016879 GO:0016881 GO:0018130 GO:0019438 GO:0019752 GO:0022414 GO:0031974 GO:0032501 GO:0032502 GO:0034641 GO:0042398 GO:0042558 GO:0042559 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043436 GO:0043603 GO:0043604 GO:0043648 GO:0043650 GO:0044237 GO:0044249 GO:0044271 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044429 GO:0044444 GO:0044446 GO:0044464 GO:0046394 GO:0046452 GO:0046483 GO:0046900 GO:0046901 GO:0048316 GO:0048608 GO:0048731 GO:0048856 GO:0051186 GO:0051188 GO:0061458 GO:0070013 GO:0071704 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00790 ko01100 map00790 map01100

Pfam Domains

Protein Families

Mur_ligase_M

Protein Analysis

441

Amino Acids

47.11

Weight (kDa)

6.07

Isoelectric Point (pI)

40.51

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Mur_ligase_M	PF08245	49 - 230	7.6e-07	Mur ligase middle domain
Mur_ligase_C	PF02875	272 - 347	3.9e-06	Mur ligase, glutamate ligase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0012917)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G41480
fragaria_vesca	FvH4_7g31010 FvH4_7g31010
malus_domestica	MD07G1285000.v1.1
prunus_persica	Prupe.2G307100_v2.0.a1
pyrus_communis	pycom07g25870
rosa_chinensis	RchiOBHm_Chr1g0380091
rosa_laevigata	RLG00000026333
rosa_multiflora	Rmu_sc0001232.1_g000007 Rmu_sc0004184.1_g000001
rosa_roxburghii	Rroxscaffold_4G00279720
rosa_rugosa	Rorug01G0417300 Rorug01G0417400
rosa_samantha	Rh1AG442500 Rh1BG397500 Rh1CG410900 Rh1DG428300
rosa_wichuraiana	Rw1G038420

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	72
AciI	CCGC	2 cut(s)	44, 700
AclWI	GGATC	6 cut(s)	156, 723, 736, 1060, 1073, 1146
AcsI	RAATTY	2 cut(s)	456, 717
AcuI	CTGAAG	2 cut(s)	644, 1232
AcvI	CACGTG	1 cut(s)	275
AfaI	GTAC	1 cut(s)	178
AfeI	AGCGCT	1 cut(s)	867
AfiI	CCNNNNNNNGG	1 cut(s)	902
AgeI	ACCGGT	2 cut(s)	94, 1251
AgsI	TTSAA	4 cut(s)	53, 167, 452, 593
AluBI	AGCT	7 cut(s)	300, 607, 619, 797, 1007, 1073, 1307
AluI	AGCT	7 cut(s)	300, 607, 619, 797, 1007, 1073, 1307
Alw26I	GTCTC	1 cut(s)	701
AlwI	GGATC	6 cut(s)	156, 723, 736, 1060, 1073, 1146
AlwNI	CAGNNNCTG	1 cut(s)	674
Aor51HI	AGCGCT	1 cut(s)	867
ApeKI	GCWGC	5 cut(s)	311, 350, 668, 686, 1040
ApoI	RAATTY	2 cut(s)	456, 717
AsiGI	ACCGGT	2 cut(s)	94, 1251
Asp700I	GAANNNNTTC	1 cut(s)	456
AspLEI	GCGC	2 cut(s)	272, 868
AspS9I	GGNCC	2 cut(s)	431, 1249
AsuHPI	GGTGA	2 cut(s)	347, 644
AvaII	GGWCC	2 cut(s)	431, 1249
BamHI	GGATCC	2 cut(s)	728, 1065
BanII	GRGCYC	1 cut(s)	844
BbrPI	CACGTG	1 cut(s)	275
BbvI	GCAGC	5 cut(s)	298, 337, 680, 698, 1052
BccI	CCATC	3 cut(s)	705, 830, 865
BcgI	CGANNNNNNTGC	2 cut(s)	1029, 1063
BcoDI	GTCTC	1 cut(s)	701
BfaI	CTAG	4 cut(s)	39, 861, 930, 947
BfmI	CTRYAG	2 cut(s)	492, 1038
BfoI	RGCGCY	1 cut(s)	869
BfuAI	ACCTGC	1 cut(s)	72
BisI	GCNGC	5 cut(s)	312, 351, 669, 687, 1041
BlsI	GCNGC	5 cut(s)	313, 352, 670, 688, 1042
Bme18I	GGWCC	2 cut(s)	431, 1249
BmgT120I	GGNCC	2 cut(s)	431, 1249
BmiI	GGNNCC	5 cut(s)	730, 841, 1067, 1250, 1279
BmsI	GCATC	3 cut(s)	268, 323, 512
Bpu10I	CCTNAGC	1 cut(s)	798
BpuEI	CTTGAG	1 cut(s)	210
BsaAI	YACGTR	1 cut(s)	275
BsaWI	WCCGGW	2 cut(s)	94, 1251
BsaXI	ACNNNNNCTCC	2 cut(s)	681, 711
Bsc4I	CCNNNNNNNGG	1 cut(s)	902
Bse118I	RCCGGY	2 cut(s)	94, 1251
Bse1I	ACTGG	4 cut(s)	416, 517, 745, 1302
Bse3DI	GCAATG	3 cut(s)	375, 387, 930
BseGI	GGATG	2 cut(s)	631, 716
BseLI	CCNNNNNNNGG	1 cut(s)	902
BseMI	GCAATG	3 cut(s)	375, 387, 930
BseMII	CTCAG	2 cut(s)	812, 1179
BseNI	ACTGG	4 cut(s)	416, 517, 745, 1302
BseRI	GAGGAG	1 cut(s)	353
BseXI	GCAGC	5 cut(s)	298, 337, 680, 698, 1052
BseYI	CCCAGC	2 cut(s)	257, 1016
BsgI	GTGCAG	2 cut(s)	336, 705
BshTI	ACCGGT	2 cut(s)	94, 1251
BsiSI	CCGG	2 cut(s)	95, 1252
BslFI	GGGAC	3 cut(s)	611, 832, 1262
BslI	CCNNNNNNNGG	1 cut(s)	902
BsmAI	GTCTC	1 cut(s)	701
BsmFI	GGGAC	3 cut(s)	611, 832, 1262
BsmI	GAATGC	2 cut(s)	209, 670
Bsp1286I	GDGCHC	1 cut(s)	844
Bsp143I	GATC	5 cut(s)	148, 728, 940, 1065, 1138
BspACI	CCGC	2 cut(s)	44, 700
BspCNI	CTCAG	2 cut(s)	811, 1180
BspHI	TCATGA	1 cut(s)	1315
BspLI	GGNNCC	5 cut(s)	730, 841, 1067, 1250, 1279
BspMAI	CTGCAG	1 cut(s)	1042
BspMI	ACCTGC	1 cut(s)	72
BspPI	GGATC	6 cut(s)	156, 723, 736, 1060, 1073, 1146
BsrDI	GCAATG	3 cut(s)	375, 387, 930
BsrFI	RCCGGY	2 cut(s)	94, 1251
BsrI	ACTGG	4 cut(s)	416, 517, 745, 1302
BssAI	RCCGGY	2 cut(s)	94, 1251
BssMI	GATC	5 cut(s)	148, 728, 940, 1065, 1138
Bst4CI	ACNGT	1 cut(s)	880
BstAPI	GCANNNNNTGC	2 cut(s)	278, 674
BstBAI	YACGTR	1 cut(s)	275
BstC8I	GCNNGC	2 cut(s)	44, 545
BstDEI	CTNAG	3 cut(s)	706, 798, 1188
BstF5I	GGATG	2 cut(s)	631, 716
BstH2I	RGCGCY	1 cut(s)	869
BstHHI	GCGC	2 cut(s)	272, 868
BstKTI	GATC	5 cut(s)	151, 731, 943, 1068, 1141
BstMAI	GTCTC	1 cut(s)	701
BstMBI	GATC	5 cut(s)	148, 728, 940, 1065, 1138
BstMWI	GCNNNNNNNGC	4 cut(s)	278, 674, 683, 1013
BstNSI	RCATGY	3 cut(s)	547, 643, 983
BstSFI	CTRYAG	2 cut(s)	492, 1038
BstV1I	GCAGC	5 cut(s)	298, 337, 680, 698, 1052
BstX2I	RGATCY	2 cut(s)	728, 1065
BstYI	RGATCY	2 cut(s)	728, 1065
BtgZI	GCGATG	1 cut(s)	997
BtsCI	GGATG	2 cut(s)	631, 716
BtsI	GCAGTG	1 cut(s)	198
BtsIMutI	CAGTG	6 cut(s)	81, 198, 423, 876, 1185, 1295
BveI	ACCTGC	1 cut(s)	72
Cac8I	GCNNGC	2 cut(s)	44, 545
CaiI	CAGNNNCTG	1 cut(s)	674
CciI	TCATGA	1 cut(s)	1315
CfoI	GCGC	2 cut(s)	272, 868
Cfr10I	RCCGGY	2 cut(s)	94, 1251
Cfr13I	GGNCC	2 cut(s)	431, 1249
Csp6I	GTAC	1 cut(s)	177
CspAI	ACCGGT	2 cut(s)	94, 1251
CviAII	CATG	6 cut(s)	152, 407, 544, 640, 980, 1316
CviQI	GTAC	1 cut(s)	177
DdeI	CTNAG	3 cut(s)	706, 798, 1188
DpnI	GATC	5 cut(s)	150, 730, 942, 1067, 1140
DpnII	GATC	5 cut(s)	148, 728, 940, 1065, 1138
Eco24I	GRGCYC	1 cut(s)	844
Eco47I	GGWCC	2 cut(s)	431, 1249
Eco47III	AGCGCT	1 cut(s)	867
Eco57I	CTGAAG	2 cut(s)	644, 1232
Eco72I	CACGTG	1 cut(s)	275
EcoT38I	GRGCYC	1 cut(s)	844
FaeI	CATG	6 cut(s)	155, 410, 547, 643, 983, 1319
FaqI	GGGAC	3 cut(s)	611, 832, 1262
FatI	CATG	6 cut(s)	151, 406, 543, 639, 979, 1315
FauI	CCCGC	1 cut(s)	51
Fnu4HI	GCNGC	5 cut(s)	312, 351, 669, 687, 1041
FokI	GGATG	2 cut(s)	638, 723
FriOI	GRGCYC	1 cut(s)	844
Fsp4HI	GCNGC	5 cut(s)	312, 351, 669, 687, 1041
FspBI	CTAG	4 cut(s)	39, 861, 930, 947
GlaI	GCGC	2 cut(s)	271, 867
GluI	GCNGC	5 cut(s)	312, 351, 669, 687, 1041
GsaI	CCCAGC	2 cut(s)	261, 1020
HaeII	RGCGCY	1 cut(s)	869
HapII	CCGG	2 cut(s)	95, 1252
HhaI	GCGC	2 cut(s)	272, 868
Hin1II	CATG	6 cut(s)	155, 410, 547, 643, 983, 1319
Hin6I	GCGC	2 cut(s)	270, 866
HinP1I	GCGC	2 cut(s)	270, 866
HincII	GTYRAC	1 cut(s)	238
HindII	GTYRAC	1 cut(s)	238
HindIII	AAGCTT	1 cut(s)	1071
HinfI	GANTC	4 cut(s)	142, 396, 854, 910
HpaII	CCGG	2 cut(s)	95, 1252
HphI	GGTGA	2 cut(s)	347, 644
Hpy166II	GTNNAC	2 cut(s)	238, 1185
Hpy188I	TCNGA	6 cut(s)	304, 508, 663, 724, 999, 1227
Hpy188III	TCNNGA	8 cut(s)	146, 227, 393, 464, 896, 1103, 1164, 1316
Hpy8I	GTNNAC	2 cut(s)	238, 1185
Hpy99I	CGWCG	1 cut(s)	1109
HpyAV	CCTTC	3 cut(s)	8, 100, 1322
HpyCH4III	ACNGT	1 cut(s)	880
HpyCH4IV	ACGT	2 cut(s)	67, 274
HpyF10VI	GCNNNNNNNGC	4 cut(s)	278, 674, 683, 1013
HpyF3I	CTNAG	3 cut(s)	706, 798, 1188
HpySE526I	ACGT	2 cut(s)	67, 274
Hsp92II	CATG	6 cut(s)	155, 410, 547, 643, 983, 1319
HspAI	GCGC	2 cut(s)	270, 866
Kzo9I	GATC	5 cut(s)	148, 728, 940, 1065, 1138
LmnI	GCTCC	2 cut(s)	340, 839
Lsp1109I	GCAGC	5 cut(s)	298, 337, 680, 698, 1052
LweI	GCATC	3 cut(s)	268, 323, 512
MaeI	CTAG	4 cut(s)	39, 861, 930, 947
MaeII	ACGT	2 cut(s)	67, 274
MaeIII	GTNAC	1 cut(s)	932
MalI	GATC	5 cut(s)	150, 730, 942, 1067, 1140
MboI	GATC	5 cut(s)	148, 728, 940, 1065, 1138
MboII	GAAGA	6 cut(s)	151, 452, 780, 1091, 1225, 1251
MflI	RGATCY	2 cut(s)	728, 1065
MhlI	GDGCHC	1 cut(s)	844
MluCI	AATT	6 cut(s)	456, 717, 1082, 1142, 1260, 1269
MlyI	GAGTC	2 cut(s)	863, 904
MnlI	CCTC	8 cut(s)	184, 247, 331, 449, 892, 979, 1054, 1183
MroXI	GAANNNNTTC	1 cut(s)	456
MseI	TTAA	5 cut(s)	135, 402, 602, 1052, 1321
MslI	CAYNNNNRTG	1 cut(s)	380
MspI	CCGG	2 cut(s)	95, 1252
Mva1269I	GAATGC	2 cut(s)	209, 670
MwoI	GCNNNNNNNGC	4 cut(s)	278, 674, 683, 1013
NdeII	GATC	5 cut(s)	148, 728, 940, 1065, 1138
NlaIII	CATG	6 cut(s)	155, 410, 547, 643, 983, 1319
NlaIV	GGNNCC	5 cut(s)	730, 841, 1067, 1250, 1279
NmuCI	GTSAC	1 cut(s)	932
NspI	RCATGY	3 cut(s)	547, 643, 983
PaeI	GCATGC	1 cut(s)	547
PagI	TCATGA	1 cut(s)	1315
PctI	GAATGC	2 cut(s)	209, 670
PdmI	GAANNNNTTC	1 cut(s)	456
PfeI	GAWTC	2 cut(s)	142, 396
PinAI	ACCGGT	2 cut(s)	94, 1251
PkrI	GCNGC	5 cut(s)	313, 352, 670, 688, 1042
PleI	GAGTC	2 cut(s)	862, 904
PmaCI	CACGTG	1 cut(s)	275
PmlI	CACGTG	1 cut(s)	275
PpsI	GAGTC	2 cut(s)	862, 904
Ppu21I	YACGTR	1 cut(s)	275
PspCI	CACGTG	1 cut(s)	275
PspFI	CCCAGC	2 cut(s)	257, 1016
PspN4I	GGNNCC	5 cut(s)	730, 841, 1067, 1250, 1279
PspPI	GGNCC	2 cut(s)	431, 1249
PstI	CTGCAG	1 cut(s)	1042
PstNI	CAGNNNCTG	1 cut(s)	674
PsuI	RGATCY	2 cut(s)	728, 1065
RsaI	GTAC	1 cut(s)	178
RsaNI	GTAC	1 cut(s)	177
RseI	CAYNNNNRTG	1 cut(s)	380
SaqAI	TTAA	5 cut(s)	135, 402, 602, 1052, 1321
SatI	GCNGC	5 cut(s)	312, 351, 669, 687, 1041
Sau3AI	GATC	5 cut(s)	148, 728, 940, 1065, 1138
Sau96I	GGNCC	2 cut(s)	431, 1249
SchI	GAGTC	2 cut(s)	863, 904
SduI	GDGCHC	1 cut(s)	844
SfaNI	GCATC	3 cut(s)	268, 323, 512
SfcI	CTRYAG	2 cut(s)	492, 1038
SinI	GGWCC	2 cut(s)	431, 1249
SmiMI	CAYNNNNRTG	1 cut(s)	380
SmlI	CTYRAG	1 cut(s)	225
SmoI	CTYRAG	1 cut(s)	225
SphI	GCATGC	1 cut(s)	547
Sse9I	AATT	6 cut(s)	456, 717, 1082, 1142, 1260, 1269
SsiI	CCGC	2 cut(s)	44, 700
SspI	AATATT	1 cut(s)	828
SspMI	CTAG	4 cut(s)	39, 861, 930, 947
TaaI	ACNGT	1 cut(s)	880
TaiI	ACGT	2 cut(s)	70, 277
TaqII	GACCGA	2 cut(s)	307, 1016
TasI	AATT	6 cut(s)	456, 717, 1082, 1142, 1260, 1269
TfiI	GAWTC	2 cut(s)	142, 396
Tru1I	TTAA	5 cut(s)	135, 402, 602, 1052, 1321
Tru9I	TTAA	5 cut(s)	135, 402, 602, 1052, 1321
TscAI	CASTG	6 cut(s)	81, 205, 423, 883, 1192, 1302
TseFI	GTSAC	1 cut(s)	932
TseI	GCWGC	5 cut(s)	311, 350, 668, 686, 1040
Tsp45I	GTSAC	1 cut(s)	932
TspDTI	ATGAA	2 cut(s)	601, 1304
TspGWI	ACGGA	1 cut(s)	741
TspRI	CASTG	6 cut(s)	81, 205, 423, 883, 1192, 1302
VpaK11BI	GGWCC	2 cut(s)	431, 1249
XapI	RAATTY	2 cut(s)	456, 717
XceI	RCATGY	3 cut(s)	547, 643, 983
XmnI	GAANNNNTTC	1 cut(s)	456
XspI	CTAG	4 cut(s)	39, 861, 930, 947

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.