Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr1

Orthogroup ID

OG0012917

Physical Location & Seq

Genomic Coordinates Forward (+)

65963161 .. 65966615

Gene Structure 3455 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw1G038420.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1584 bp

ATGAAAATTCTGAAGCTTTTCCGGCGATGCTCCGTCATTACTAACCGGACGGGCCCAATTGCCTCAGTTCGGGTCGGGTTATTGGGTAATGCGGGCCCAAAAGAGGGTTTTGACTTGATGGAGTACATGGACTCACTCAAGAACTACGAGAAATCGGGGGTTCCGGTCGGGGCGGGTACGGATTCTGACGACGGGTTCGATCTGGGTCGGATGCGGACGTTGATGGAGCTTCTGGGTCGTCCTCATTTCAAGTTCAAGGCGGTTCACATTGCCGGAACGAAAGGAAAAGGATCAACAGCTGCATTTCTGTGCAACATTTTGAGGGCAGAAGGGTATTCAGTTGGTTGTTATACTAGCCCACATATTCGAACTATCAGGGAGCGAATAACTTTGGGAAGGTCTGGTGAGCCGGTGTCGGCAAAGGCTTTGAATTCTCTGTTTCAGGAGAATAAGGAGACTCTGGATCGTGCAATGAAAGTTGAAATTGGATATATTAGTCACTTTGAGGTTCTTACTGCTCTGGCATTCACACTATTTGCTAAGAGAAGGTTGATATTGCACTCTGGACTTGCCACATCGGTCATTACTACAATAGGAGAGGAACATTTGGCTGCACTTGGGGGTTCTTTGGAAAGCATTGCAATGGCGAAGTCTGGAATCATGAAACATGGCCGCCCAGTGGTTTTGGGCGGTCCATTTCTTCCACATATTGAGTGCATTCTTCGTGATAAAGCTCTGTTGATGAGTTCTCCTGTTGTATTAGCGTCTGATACTAGAAATAGAAGCAAGATAAATGGTTTTAGCATGCACGATGGTAGACCCTTCCAATCTTGTGATATAGTGCTACAACTTGAGAGTGACTTAAAGCTGTCAATTGAATTATTGGATTTGAAACTATACATGCTTGGAAGTCACCAACTGCAGAATGCTGCAACTGCTACTTGTGCAGCACTATGTCTCCGAAACTTAGGATGGAGAATTTCAGATGCATCCATTAGAACTGGTTTACAGCAGACACATCTTCTTGGAAGAAGTCAATTTCTAACATCGAAGGAAGCTCAGGCATTGAGATTGTATGGAGCAACAATATTGCTTGATGGAGCCCACACCAAAGAATCTGCTAAAGCGCTGATGGACACTATAAAGATGACTTTCCCAGAGTCGCGATTGACTCTTGTGGTTGCAATGGCAAATGACAAAGATCATCTAGGTTTCGCAAGAGAGTTTCTTTCAGGTGGGCATCTAGAGGGTGTCCTCTGTACAGAAGCTAACATTGCTGGGGGAAAATCTCGGACAACTGCAGCGTCAGTGCTAAGAAATTGCTGGATCCAAGCTTCCAAAGAATTGGGCCTAGATATTGTTCATGATCAAATGACAGAATGTCAAAACTTTTCAATGGACCAATTAGTTGGCTTAAGCACGTTGGAAAAGAGACCATTATTGGCTGCTGTGCCTTCATTTCCGGATTCCATCAAGATTGCAAATCTGATTTCCAGAGGAAGAACTAGGGATCAATCTCATATTACTGTAATCACTGGCTCTCTACATATAGTTTCATTGACATTAGCCACCCTCAATAGTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0003674 GO:0003824 GO:0004326 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005759 GO:0006082 GO:0006575 GO:0006725 GO:0006732 GO:0006760 GO:0006761 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0008841 GO:0009058 GO:0009108 GO:0009396 GO:0009790 GO:0009791 GO:0009793 GO:0009987 GO:0010154 GO:0016053 GO:0016874 GO:0016879 GO:0016881 GO:0018130 GO:0019438 GO:0019752 GO:0022414 GO:0031974 GO:0032501 GO:0032502 GO:0034641 GO:0042398 GO:0042558 GO:0042559 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043436 GO:0043603 GO:0043604 GO:0043648 GO:0043650 GO:0044237 GO:0044249 GO:0044271 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044429 GO:0044444 GO:0044446 GO:0044464 GO:0046394 GO:0046452 GO:0046483 GO:0046900 GO:0046901 GO:0048316 GO:0048608 GO:0048731 GO:0048856 GO:0051186 GO:0051188 GO:0061458 GO:0070013 GO:0071704 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00790 ko01100 map00790 map01100

Pfam Domains

Protein Families

Mur_ligase_M

Protein Analysis

527

Amino Acids

57.3

Weight (kDa)

9.2

Isoelectric Point (pI)

46.45

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Mur_ligase_C	PF02875	360 - 424	1.9e-06	Mur ligase, glutamate ligase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0012917)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G41480
fragaria_vesca	FvH4_7g31010 FvH4_7g31010
malus_domestica	MD07G1285000.v1.1
prunus_persica	Prupe.2G307100_v2.0.a1
pyrus_communis	pycom07g25870
rosa_chinensis	RchiOBHm_Chr1g0380091
rosa_laevigata	RLG00000026333
rosa_multiflora	Rmu_sc0001232.1_g000007 Rmu_sc0004184.1_g000001
rosa_roxburghii	Rroxscaffold_4G00279720
rosa_rugosa	Rorug01G0417300 Rorug01G0417400
rosa_samantha	Rh1AG442500 Rh1BG397500 Rh1CG410900 Rh1DG428300
rosa_wichuraiana	Rw1G038420

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccI	GTMKAC	1 cut(s)	817
AccII	CGCG	1 cut(s)	1165
AccIII	TCCGGA	1 cut(s)	1462
AciI	CCGC	6 cut(s)	92, 173, 214, 260, 673, 690
AclWI	GGATC	5 cut(s)	298, 471, 1321, 1334, 1518
AcoI	YGGCCR	1 cut(s)	670
AcsI	RAATTY	3 cut(s)	6, 430, 978
AcuI	CTGAAG	1 cut(s)	32
AfaI	GTAC	3 cut(s)	125, 178, 1261
AfeI	AGCGCT	1 cut(s)	1128
AfiI	CCNNNNNNNGG	4 cut(s)	69, 103, 104, 679
AflII	CTTAAG	1 cut(s)	1414
AgsI	TTSAA	7 cut(s)	250, 256, 430, 482, 878, 892, 1395
AhdI	GACNNNNNGTC	1 cut(s)	1380
AluBI	AGCT	8 cut(s)	16, 229, 299, 734, 868, 1058, 1268, 1334
AluI	AGCT	8 cut(s)	16, 229, 299, 734, 868, 1058, 1268, 1334
Alw26I	GTCTC	3 cut(s)	449, 962, 1426
AlwI	GGATC	5 cut(s)	298, 471, 1321, 1334, 1518
Aor13HI	TCCGGA	1 cut(s)	1462
Aor51HI	AGCGCT	1 cut(s)	1128
AoxI	GGCC	4 cut(s)	52, 94, 670, 1348
ApaI	GGGCCC	2 cut(s)	56, 98
ApeKI	GCWGC	6 cut(s)	299, 611, 929, 947, 1301, 1445
ApoI	RAATTY	3 cut(s)	6, 430, 978
ArsI	GACNNNNNNTTYG	2 cut(s)	179, 211
Asp700I	GAANNNNTTC	1 cut(s)	17
AspLEI	GCGC	1 cut(s)	1129
AspS9I	GGNCC	7 cut(s)	52, 53, 94, 95, 692, 1348, 1399
AsuHPI	GGTGA	2 cut(s)	416, 905
AsuII	TTCGAA	1 cut(s)	367
AvaII	GGWCC	2 cut(s)	692, 1399
BaeGI	GKGCMC	2 cut(s)	56, 98
BamHI	GGATCC	1 cut(s)	1326
BanII	GRGCYC	3 cut(s)	56, 98, 1105
BbvI	GCAGC	6 cut(s)	286, 598, 916, 959, 1313, 1432
BccI	CCATC	7 cut(s)	112, 217, 806, 966, 1091, 1126, 1478
BclI	TGATCA	1 cut(s)	1366
BcoDI	GTCTC	3 cut(s)	449, 962, 1426
BfaI	CTAG	6 cut(s)	354, 774, 1208, 1244, 1352, 1506
BfmI	CTRYAG	2 cut(s)	920, 1299
BfoI	RGCGCY	1 cut(s)	1130
BfrI	CTTAAG	1 cut(s)	1414
BisI	GCNGC	7 cut(s)	300, 612, 673, 930, 948, 1302, 1446
BlsI	GCNGC	7 cut(s)	301, 613, 674, 931, 949, 1303, 1447
Bme18I	GGWCC	2 cut(s)	692, 1399
BmeRI	GACNNNNNGTC	1 cut(s)	1380
BmgT120I	GGNCC	7 cut(s)	52, 53, 94, 95, 692, 1348, 1399
BmiI	GGNNCC	5 cut(s)	54, 96, 162, 1102, 1328
BmrI	ACTGGG	1 cut(s)	671
BmsI	GCATC	5 cut(s)	17, 201, 976, 998, 1249
BmuI	ACTGGG	1 cut(s)	671
Bpu10I	CCTNAGC	1 cut(s)	1059
Bpu14I	TTCGAA	1 cut(s)	367
BpuEI	CTTGAG	2 cut(s)	122, 872
BsaI	GGTCTC	1 cut(s)	1426
BsaWI	WCCGGW	3 cut(s)	45, 163, 1462
BsaXI	ACNNNNNCTCC	2 cut(s)	942, 972
Bsc4I	CCNNNNNNNGG	4 cut(s)	69, 103, 104, 679
Bse118I	RCCGGY	1 cut(s)	409
Bse1I	ACTGG	3 cut(s)	677, 1006, 1540
Bse3DI	GCAATG	6 cut(s)	267, 477, 636, 648, 1191, 1272
BseAI	TCCGGA	1 cut(s)	1462
BseGI	GGATG	3 cut(s)	216, 977, 989
BseLI	CCNNNNNNNGG	4 cut(s)	69, 103, 104, 679
BseMI	GCAATG	6 cut(s)	267, 477, 636, 648, 1191, 1272
BseMII	CTCAG	2 cut(s)	78, 1073
BseNI	ACTGG	3 cut(s)	677, 1006, 1540
BseSI	GKGCMC	2 cut(s)	56, 98
BseXI	GCAGC	6 cut(s)	286, 598, 916, 959, 1313, 1432
BseYI	CCCAGC	1 cut(s)	1277
BsgI	GTGCAG	2 cut(s)	597, 966
Bsh1236I	CGCG	1 cut(s)	1165
Bsh1285I	CGRYCG	1 cut(s)	168
BshFI	GGCC	4 cut(s)	54, 96, 672, 1350
BsiEI	CGRYCG	1 cut(s)	168
BsiSI	CCGG	6 cut(s)	22, 46, 164, 273, 410, 1463
BslI	CCNNNNNNNGG	4 cut(s)	69, 103, 104, 679
BsmAI	GTCTC	3 cut(s)	449, 962, 1426
BsmI	GAATGC	3 cut(s)	524, 717, 931
BsnI	GGCC	4 cut(s)	54, 96, 672, 1350
Bso31I	GGTCTC	1 cut(s)	1426
Bsp119I	TTCGAA	1 cut(s)	367
Bsp120I	GGGCCC	2 cut(s)	52, 94
Bsp1286I	GDGCHC	3 cut(s)	56, 98, 1105
Bsp13I	TCCGGA	1 cut(s)	1462
Bsp1407I	TGTACA	1 cut(s)	1259
Bsp143I	GATC	7 cut(s)	199, 290, 463, 1201, 1326, 1366, 1510
Bsp68I	TCGCGA	1 cut(s)	1165
BspACI	CCGC	6 cut(s)	92, 173, 214, 260, 673, 690
BspANI	GGCC	4 cut(s)	54, 96, 672, 1350
BspCNI	CTCAG	2 cut(s)	77, 1072
BspEI	TCCGGA	1 cut(s)	1462
BspFNI	CGCG	1 cut(s)	1165
BspHI	TCATGA	2 cut(s)	660, 1363
BspLI	GGNNCC	5 cut(s)	54, 96, 162, 1102, 1328
BspMAI	CTGCAG	2 cut(s)	924, 1303
BspPI	GGATC	5 cut(s)	298, 471, 1321, 1334, 1518
BspT104I	TTCGAA	1 cut(s)	367
BspTI	CTTAAG	1 cut(s)	1414
BspTNI	GGTCTC	1 cut(s)	1426
BsrDI	GCAATG	6 cut(s)	267, 477, 636, 648, 1191, 1272
BsrFI	RCCGGY	1 cut(s)	409
BsrGI	TGTACA	1 cut(s)	1259
BsrI	ACTGG	3 cut(s)	677, 1006, 1540
BssAI	RCCGGY	1 cut(s)	409
BssMI	GATC	7 cut(s)	199, 290, 463, 1201, 1326, 1366, 1510
Bst4CI	ACNGT	1 cut(s)	1528
BstAFI	CTTAAG	1 cut(s)	1414
BstAUI	TGTACA	1 cut(s)	1259
BstBI	TTCGAA	1 cut(s)	367
BstC8I	GCNNGC	2 cut(s)	94, 806
BstDEI	CTNAG	5 cut(s)	64, 540, 967, 1059, 1313
BstF5I	GGATG	3 cut(s)	216, 977, 989
BstFNI	CGCG	1 cut(s)	1165
BstH2I	RGCGCY	1 cut(s)	1130
BstHHI	GCGC	1 cut(s)	1129
BstKTI	GATC	7 cut(s)	202, 293, 466, 1204, 1329, 1369, 1513
BstMAI	GTCTC	3 cut(s)	449, 962, 1426
BstMBI	GATC	7 cut(s)	199, 290, 463, 1201, 1326, 1366, 1510
BstMCI	CGRYCG	1 cut(s)	168
BstMWI	GCNNNNNNNGC	4 cut(s)	22, 935, 944, 1274
BstNSI	RCATGY	2 cut(s)	808, 904
BstSFI	CTRYAG	2 cut(s)	920, 1299
BstSLI	GKGCMC	2 cut(s)	56, 98
BstUI	CGCG	1 cut(s)	1165
BstV1I	GCAGC	6 cut(s)	286, 598, 916, 959, 1313, 1432
BstX2I	RGATCY	1 cut(s)	1326
BstXI	CCANNNNNNTGG	2 cut(s)	1345, 1409
BstYI	RGATCY	1 cut(s)	1326
BsuRI	GGCC	4 cut(s)	54, 96, 672, 1350
BtgZI	GCGATG	1 cut(s)	40
BtsCI	GGATG	3 cut(s)	216, 977, 989
BtsIMutI	CAGTG	3 cut(s)	684, 1314, 1533
BtuMI	TCGCGA	1 cut(s)	1165
Cac8I	GCNNGC	2 cut(s)	94, 806
CciI	TCATGA	2 cut(s)	660, 1363
CfoI	GCGC	1 cut(s)	1129
Cfr10I	RCCGGY	1 cut(s)	409
Cfr13I	GGNCC	7 cut(s)	52, 53, 94, 95, 692, 1348, 1399
CseI	GACGC	2 cut(s)	753, 1293
Csp6I	GTAC	3 cut(s)	124, 177, 1260
CviAII	CATG	6 cut(s)	127, 661, 668, 805, 901, 1364
CviQI	GTAC	3 cut(s)	124, 177, 1260
DdeI	CTNAG	5 cut(s)	64, 540, 967, 1059, 1313
DpnI	GATC	7 cut(s)	201, 292, 465, 1203, 1328, 1368, 1512
DpnII	GATC	7 cut(s)	199, 290, 463, 1201, 1326, 1366, 1510
DriI	GACNNNNNGTC	1 cut(s)	1380
EaeI	YGGCCR	1 cut(s)	670
Eam1105I	GACNNNNNGTC	1 cut(s)	1380
Eco24I	GRGCYC	3 cut(s)	56, 98, 1105
Eco31I	GGTCTC	1 cut(s)	1426
Eco47I	GGWCC	2 cut(s)	692, 1399
Eco47III	AGCGCT	1 cut(s)	1128
Eco57I	CTGAAG	1 cut(s)	32
EcoRI	GAATTC	1 cut(s)	430
EcoT22I	ATGCAT	1 cut(s)	991
EcoT38I	GRGCYC	3 cut(s)	56, 98, 1105
FaeI	CATG	6 cut(s)	130, 664, 671, 808, 904, 1367
FatI	CATG	6 cut(s)	126, 660, 667, 804, 900, 1363
FauI	CCCGC	2 cut(s)	85, 166
FbaI	TGATCA	1 cut(s)	1366
FblI	GTMKAC	1 cut(s)	817
Fnu4HI	GCNGC	7 cut(s)	300, 612, 673, 930, 948, 1302, 1446
FokI	GGATG	3 cut(s)	223, 976, 984
FriOI	GRGCYC	3 cut(s)	56, 98, 1105
Fsp4HI	GCNGC	7 cut(s)	300, 612, 673, 930, 948, 1302, 1446
FspBI	CTAG	6 cut(s)	354, 774, 1208, 1244, 1352, 1506
GlaI	GCGC	1 cut(s)	1128
GluI	GCNGC	7 cut(s)	300, 612, 673, 930, 948, 1302, 1446
GsaI	CCCAGC	1 cut(s)	1281
HaeII	RGCGCY	1 cut(s)	1130
HaeIII	GGCC	4 cut(s)	54, 96, 672, 1350
HapII	CCGG	6 cut(s)	22, 46, 164, 273, 410, 1463
HgaI	GACGC	2 cut(s)	753, 1293
HhaI	GCGC	1 cut(s)	1129
Hin1II	CATG	6 cut(s)	130, 664, 671, 808, 904, 1367
Hin6I	GCGC	1 cut(s)	1127
HinP1I	GCGC	1 cut(s)	1127
HindIII	AAGCTT	2 cut(s)	14, 1332
HinfI	GANTC	8 cut(s)	131, 182, 457, 657, 1115, 1160, 1171, 1466
HpaII	CCGG	6 cut(s)	22, 46, 164, 273, 410, 1463
HphI	GGTGA	2 cut(s)	416, 905
Hpy166II	GTNNAC	3 cut(s)	265, 818, 1007
Hpy188I	TCNGA	8 cut(s)	12, 187, 210, 769, 962, 985, 1293, 1488
Hpy8I	GTNNAC	3 cut(s)	265, 818, 1007
Hpy99I	CGWCG	1 cut(s)	194
HpyAV	CCTTC	6 cut(s)	323, 390, 540, 832, 1045, 1464
HpyCH4III	ACNGT	1 cut(s)	1528
HpyCH4IV	ACGT	2 cut(s)	218, 1421
HpyF10VI	GCNNNNNNNGC	4 cut(s)	22, 935, 944, 1274
HpyF3I	CTNAG	5 cut(s)	64, 540, 967, 1059, 1313
HpySE526I	ACGT	2 cut(s)	218, 1421
Hsp92II	CATG	6 cut(s)	130, 664, 671, 808, 904, 1367
HspAI	GCGC	1 cut(s)	1127
Kpn2I	TCCGGA	1 cut(s)	1462
Ksp22I	TGATCA	1 cut(s)	1366
Kzo9I	GATC	7 cut(s)	199, 290, 463, 1201, 1326, 1366, 1510
LmnI	GCTCC	5 cut(s)	35, 226, 379, 1079, 1100
Lsp1109I	GCAGC	6 cut(s)	286, 598, 916, 959, 1313, 1432
LweI	GCATC	5 cut(s)	17, 201, 976, 998, 1249
MaeI	CTAG	6 cut(s)	354, 774, 1208, 1244, 1352, 1506
MaeII	ACGT	2 cut(s)	218, 1421
MaeIII	GTNAC	3 cut(s)	497, 857, 911
MalI	GATC	7 cut(s)	201, 292, 465, 1203, 1328, 1368, 1512
MboI	GATC	7 cut(s)	199, 290, 463, 1201, 1326, 1366, 1510
MboII	GAAGA	5 cut(s)	692, 713, 1013, 1041, 1512
MfeI	CAATTG	2 cut(s)	57, 873
MflI	RGATCY	1 cut(s)	1326
MhlI	GDGCHC	3 cut(s)	56, 98, 1105
MlyI	GAGTC	4 cut(s)	125, 451, 1165, 1169
MmeI	TCCRAC	2 cut(s)	188, 1404
Mph1103I	ATGCAT	1 cut(s)	991
MroI	TCCGGA	1 cut(s)	1462
MroXI	GAANNNNTTC	1 cut(s)	17
MseI	TTAA	3 cut(s)	863, 1415, 1582
MslI	CAYNNNNRTG	2 cut(s)	307, 641
MspA1I	CMGCKG	1 cut(s)	299
MspCI	CTTAAG	1 cut(s)	1414
MspI	CCGG	6 cut(s)	22, 46, 164, 273, 410, 1463
MunI	CAATTG	2 cut(s)	57, 873
Mva1269I	GAATGC	3 cut(s)	524, 717, 931
MvnI	CGCG	1 cut(s)	1165
MwoI	GCNNNNNNNGC	4 cut(s)	22, 935, 944, 1274
NdeII	GATC	7 cut(s)	199, 290, 463, 1201, 1326, 1366, 1510
NlaIII	CATG	6 cut(s)	130, 664, 671, 808, 904, 1367
NlaIV	GGNNCC	5 cut(s)	54, 96, 162, 1102, 1328
NmuCI	GTSAC	3 cut(s)	497, 857, 911
NruI	TCGCGA	1 cut(s)	1165
NsiI	ATGCAT	1 cut(s)	991
NspI	RCATGY	2 cut(s)	808, 904
NspV	TTCGAA	1 cut(s)	367
PaeI	GCATGC	1 cut(s)	808
PagI	TCATGA	2 cut(s)	660, 1363
PcsI	WCGNNNNNNNCGW	1 cut(s)	195
PctI	GAATGC	3 cut(s)	524, 717, 931
PdmI	GAANNNNTTC	1 cut(s)	17
PfeI	GAWTC	4 cut(s)	182, 657, 1115, 1466
PkrI	GCNGC	7 cut(s)	301, 613, 674, 931, 949, 1303, 1447
PleI	GAGTC	4 cut(s)	125, 451, 1165, 1168
PpsI	GAGTC	4 cut(s)	125, 451, 1165, 1168
PspFI	CCCAGC	1 cut(s)	1277
PspN4I	GGNNCC	5 cut(s)	54, 96, 162, 1102, 1328
PspOMI	GGGCCC	2 cut(s)	52, 94
PspPI	GGNCC	7 cut(s)	52, 53, 94, 95, 692, 1348, 1399
PstI	CTGCAG	2 cut(s)	924, 1303
PsuI	RGATCY	1 cut(s)	1326
PvuII	CAGCTG	1 cut(s)	299
RruI	TCGCGA	1 cut(s)	1165
RsaI	GTAC	3 cut(s)	125, 178, 1261
RsaNI	GTAC	3 cut(s)	124, 177, 1260
RseI	CAYNNNNRTG	2 cut(s)	307, 641
SaqAI	TTAA	3 cut(s)	863, 1415, 1582
SatI	GCNGC	7 cut(s)	300, 612, 673, 930, 948, 1302, 1446
Sau3AI	GATC	7 cut(s)	199, 290, 463, 1201, 1326, 1366, 1510
Sau96I	GGNCC	7 cut(s)	52, 53, 94, 95, 692, 1348, 1399
SchI	GAGTC	4 cut(s)	125, 451, 1165, 1169
SduI	GDGCHC	3 cut(s)	56, 98, 1105
SfaNI	GCATC	5 cut(s)	17, 201, 976, 998, 1249
SfcI	CTRYAG	2 cut(s)	920, 1299
SfuI	TTCGAA	1 cut(s)	367
SinI	GGWCC	2 cut(s)	692, 1399
SmiMI	CAYNNNNRTG	2 cut(s)	307, 641
SmlI	CTYRAG	3 cut(s)	137, 851, 1414
SmoI	CTYRAG	3 cut(s)	137, 851, 1414
SphI	GCATGC	1 cut(s)	808
SsiI	CCGC	6 cut(s)	92, 173, 214, 260, 673, 690
SspI	AATATT	1 cut(s)	1089
SspMI	CTAG	6 cut(s)	354, 774, 1208, 1244, 1352, 1506
TaaI	ACNGT	1 cut(s)	1528
TaiI	ACGT	2 cut(s)	221, 1424
TaqI	TCGA	3 cut(s)	198, 367, 1049
TaqII	GACCGA	1 cut(s)	568
TatI	WGTACW	2 cut(s)	123, 1259
TauI	GCSGC	1 cut(s)	675
TfiI	GAWTC	4 cut(s)	182, 657, 1115, 1466
Tru1I	TTAA	3 cut(s)	863, 1415, 1582
Tru9I	TTAA	3 cut(s)	863, 1415, 1582
TscAI	CASTG	3 cut(s)	684, 1314, 1540
TseFI	GTSAC	3 cut(s)	497, 857, 911
TseI	GCWGC	6 cut(s)	299, 611, 929, 947, 1301, 1445
Tsp45I	GTSAC	3 cut(s)	497, 857, 911
TspDTI	ATGAA	6 cut(s)	17, 488, 677, 1352, 1446, 1545
TspGWI	ACGGA	2 cut(s)	22, 194
TspRI	CASTG	3 cut(s)	684, 1314, 1540
Vha464I	CTTAAG	1 cut(s)	1414
VpaK11BI	GGWCC	2 cut(s)	692, 1399
XapI	RAATTY	3 cut(s)	6, 430, 978
XbaI	TCTAGA	1 cut(s)	1243
XceI	RCATGY	2 cut(s)	808, 904
XmiI	GTMKAC	1 cut(s)	817
XmnI	GAANNNNTTC	1 cut(s)	17
XspI	CTAG	6 cut(s)	354, 774, 1208, 1244, 1352, 1506
Zsp2I	ATGCAT	1 cut(s)	991

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.