Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000004

Orthogroup ID

OG0012917

Physical Location & Seq

Genomic Coordinates Reverse (-)

2437239 .. 2441058

Gene Structure 3820 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_4G00279720.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1668 bp

ATGAAAATTCTCAAGCTTTTCCGGCAATGCTCCGTCATTGCTAACCGGACGGGCCCAATTGCCTCAGTTCGGGTCGGGCTATTGGGTAATGCGGGGCCCAAAAGAGGGTTTTGCTGGAGCACAGAGGATTCGGAGCTGAAGGACTTGATGGAGTACATGGACTCACTCAAGAACTACGAGAAATCTGGGGTTCCGGTCGGGGCGGGTACGGATTCTGACGACGGGTTCGACCTGGGTCGGATGCGGACGTTGATGGAGCTTCTGGGTCGTCCTCATTTCAAGTTCAAGGCGGTTCATATTGCCGGAACGAAAGGAAAAGGATCAACAGCTGCATTTCTGTGCAACATTTTGAGGGCAGAAGGGTATTCAGTTGGTTGTTATACTAGCCCACATATTCGAACTATCACGGAGCGAATAACTTTGGGAAGGTCTGGTGAGCCGGTGTCGGCAAAGGCTTTGAATTCTCTGTTTCAAGAGAATAAGGAGACTCTGGATCGTGCAATGAAAGTTGAAAATGGATATATTAGTCACTTTGAGGTTCTTACTGCTCTGGCATTCACACTATTTGCTAAAGAGAAGGTTGATATTGCAGTGATTGAGGCTGGCTTGGGGGGTGCACGGGATGCAACTAATGTCATTCCTAGCTCTGGACTTGCCACATCGGTCATTACTACAATAGGAGAGGAACATTTGGCTGCACTTGGAGGTTCTTTGGAAAGCATTGCAATGGCAAAGTCTGGAATCATGAAACATGGCCGCCCAGTGGTTTTGGGCGGTCCATTTCTTCCACATATTGAGTGCATTCTTCGTGATAAAGCTCTGTTGATGAGTTCTCCTGTAGTATTAGCGTCTGATACTAGAAATAGAAGCAAGATAAATGGTTTTAGCATGCACGATGGTAGACCCTTCCAATCTTGTGATATAGTGCTACAACTTGAGAGTGACTTAAAGCTGTCAATTGAATTATTGGATTTGAAACTATACATGCTTGGAAGTCACCAACTGCAGAATGCTGCAACTGCTAGTTGTGCAGCACTGTGTCTCCGAAACTTAGGATGGAGAATTTCAGATGCATCCATTAGAACTGGTTTACAGCAGACGCATCTTCTTGGAAGAAGTCAATTTCTAACATCGAAGGAAGCTCAGGCATTGGGATTGTCTGGAGCAACAATATTGCTTGATGGAGCCCACACCAAAGAATCTGCTAAAGCGCTGATGGAAACCATAAAGATGACTTTCCCAGAGTCGCGATTGACTCTTGTGGTTGCAATGGCAAATGACAAAGATCATCTAGGTTTCGCAAGAGAGTTTCTTTCAGGTGGGCATCTAGAGAGTGTCCTCTGTACAGAAGCTAACATTGCTGGGGGAAAATCTCGGACAACTGCAGCGTCGGTGCTTAGAAATTGCTGGATCCAAGCTTCCAAAGAATTGGGCCTAGATATTGTTCATGATCAAATGACAGAATGTCAAAACTTTCCAATGGACCAATTAGTTGGCTTAAGCACGTCGGAAAAGAGACCATTATTGGCTGCTGTGCCTTCATTTCCAGATTCCATCAAGATTGCAAATCTGATTTCCAGAGGAAGAACTAGGGATCAATCTCATATTACTGTAATCACTGGCTCTCTACATATAGTTTCATTGACATTAGCCACCCTCAATAGTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0003674 GO:0003824 GO:0004326 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005759 GO:0006082 GO:0006575 GO:0006725 GO:0006732 GO:0006760 GO:0006761 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0008841 GO:0009058 GO:0009108 GO:0009396 GO:0009790 GO:0009791 GO:0009793 GO:0009987 GO:0010154 GO:0016053 GO:0016874 GO:0016879 GO:0016881 GO:0018130 GO:0019438 GO:0019752 GO:0022414 GO:0031974 GO:0032501 GO:0032502 GO:0034641 GO:0042398 GO:0042558 GO:0042559 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043436 GO:0043603 GO:0043604 GO:0043648 GO:0043650 GO:0044237 GO:0044249 GO:0044271 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044429 GO:0044444 GO:0044446 GO:0044464 GO:0046394 GO:0046452 GO:0046483 GO:0046900 GO:0046901 GO:0048316 GO:0048608 GO:0048731 GO:0048856 GO:0051186 GO:0051188 GO:0061458 GO:0070013 GO:0071704 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00790 ko01100 map00790 map01100

Pfam Domains

Protein Families

Mur_ligase_M

Protein Analysis

555

Amino Acids

59.82

Weight (kDa)

8.54

Isoelectric Point (pI)

46.08

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Mur_ligase_M	PF08245	100 - 345	9.8e-08	Mur ligase middle domain
Mur_ligase_C	PF02875	387 - 454	6e-07	Mur ligase, glutamate ligase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0012917)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G41480
fragaria_vesca	FvH4_7g31010 FvH4_7g31010
malus_domestica	MD07G1285000.v1.1
prunus_persica	Prupe.2G307100_v2.0.a1
pyrus_communis	pycom07g25870
rosa_chinensis	RchiOBHm_Chr1g0380091
rosa_laevigata	RLG00000026333
rosa_multiflora	Rmu_sc0001232.1_g000007 Rmu_sc0004184.1_g000001
rosa_roxburghii	Rroxscaffold_4G00279720
rosa_rugosa	Rorug01G0417300 Rorug01G0417400
rosa_samantha	Rh1AG442500 Rh1BG397500 Rh1CG410900 Rh1DG428300
rosa_wichuraiana	Rw1G038420

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccI	GTMKAC	1 cut(s)	901
AccII	CGCG	1 cut(s)	1249
AciI	CCGC	6 cut(s)	92, 203, 244, 290, 757, 774
AclWI	GGATC	5 cut(s)	328, 501, 1405, 1418, 1602
AcoI	YGGCCR	1 cut(s)	754
AcsI	RAATTY	3 cut(s)	6, 460, 1062
AcuI	CTGAAG	1 cut(s)	158
AfaI	GTAC	3 cut(s)	155, 208, 1345
AfeI	AGCGCT	1 cut(s)	1212
AfiI	CCNNNNNNNGG	5 cut(s)	69, 104, 105, 647, 763
AflII	CTTAAG	1 cut(s)	1498
AgsI	TTSAA	7 cut(s)	280, 286, 460, 473, 512, 962, 976
AhdI	GACNNNNNGTC	1 cut(s)	1464
AjiI	CACGTC	1 cut(s)	1506
AjnI	CCWGG	1 cut(s)	231
Alw21I	GWGCWC	2 cut(s)	122, 619
Alw26I	GTCTC	3 cut(s)	479, 1046, 1510
Alw44I	GTGCAC	1 cut(s)	615
AlwI	GGATC	5 cut(s)	328, 501, 1405, 1418, 1602
Aor51HI	AGCGCT	1 cut(s)	1212
AoxI	GGCC	4 cut(s)	52, 95, 754, 1432
ApaI	GGGCCC	2 cut(s)	56, 99
ApaLI	GTGCAC	1 cut(s)	615
ApeKI	GCWGC	6 cut(s)	329, 695, 1013, 1031, 1385, 1529
ApoI	RAATTY	3 cut(s)	6, 460, 1062
ArsI	GACNNNNNNTTYG	2 cut(s)	209, 241
AspLEI	GCGC	1 cut(s)	1213
AspS9I	GGNCC	7 cut(s)	52, 53, 95, 96, 776, 1432, 1483
AsuHPI	GGTGA	2 cut(s)	446, 989
AsuII	TTCGAA	1 cut(s)	397
AvaII	GGWCC	2 cut(s)	776, 1483
BaeGI	GKGCMC	3 cut(s)	56, 99, 619
BamHI	GGATCC	1 cut(s)	1410
BanII	GRGCYC	3 cut(s)	56, 99, 1189
Bbv12I	GWGCWC	2 cut(s)	122, 619
BbvI	GCAGC	6 cut(s)	316, 682, 1000, 1043, 1397, 1516
BccI	CCATC	7 cut(s)	142, 247, 890, 1050, 1175, 1210, 1562
BciT130I	CCWGG	1 cut(s)	233
BclI	TGATCA	1 cut(s)	1450
BcoDI	GTCTC	3 cut(s)	479, 1046, 1510
BfaI	CTAG	8 cut(s)	384, 642, 858, 1023, 1292, 1328, 1436, 1590
BfmI	CTRYAG	3 cut(s)	837, 1004, 1383
BfoI	RGCGCY	1 cut(s)	1214
BfrI	CTTAAG	1 cut(s)	1498
BisI	GCNGC	7 cut(s)	330, 696, 757, 1014, 1032, 1386, 1530
BlsI	GCNGC	7 cut(s)	331, 697, 758, 1015, 1033, 1387, 1531
Bme1390I	CCNGG	1 cut(s)	233
Bme18I	GGWCC	2 cut(s)	776, 1483
BmeRI	GACNNNNNGTC	1 cut(s)	1464
BmgBI	CACGTC	1 cut(s)	1506
BmgT120I	GGNCC	7 cut(s)	52, 53, 95, 96, 776, 1432, 1483
BmiI	GGNNCC	6 cut(s)	54, 96, 97, 192, 1186, 1412
BmrFI	CCNGG	1 cut(s)	233
BmrI	ACTGGG	1 cut(s)	755
BmsI	GCATC	6 cut(s)	231, 613, 1060, 1082, 1111, 1333
BmuI	ACTGGG	1 cut(s)	755
BoxI	GACNNNNGTC	1 cut(s)	234
BpmI	CTGGAG	2 cut(s)	136, 1182
Bpu10I	CCTNAGC	1 cut(s)	1143
Bpu14I	TTCGAA	1 cut(s)	397
BpuEI	CTTGAG	2 cut(s)	152, 956
BsaI	GGTCTC	1 cut(s)	1510
BsaJI	CCNNGG	1 cut(s)	232
BsaWI	WCCGGW	2 cut(s)	45, 193
BsaXI	ACNNNNNCTCC	2 cut(s)	1026, 1056
Bsc4I	CCNNNNNNNGG	5 cut(s)	69, 104, 105, 647, 763
Bse118I	RCCGGY	1 cut(s)	439
Bse1I	ACTGG	3 cut(s)	761, 1090, 1624
Bse3DI	GCAATG	7 cut(s)	32, 36, 507, 720, 732, 1275, 1356
BseBI	CCWGG	1 cut(s)	233
BseDI	CCNNGG	1 cut(s)	232
BseGI	GGATG	4 cut(s)	246, 628, 1061, 1073
BseLI	CCNNNNNNNGG	5 cut(s)	69, 104, 105, 647, 763
BseMI	GCAATG	7 cut(s)	32, 36, 507, 720, 732, 1275, 1356
BseMII	CTCAG	2 cut(s)	78, 1157
BseNI	ACTGG	3 cut(s)	761, 1090, 1624
BseSI	GKGCMC	3 cut(s)	56, 99, 619
BseXI	GCAGC	6 cut(s)	316, 682, 1000, 1043, 1397, 1516
BseYI	CCCAGC	1 cut(s)	1361
BsgI	GTGCAG	2 cut(s)	681, 1050
Bsh1236I	CGCG	1 cut(s)	1249
Bsh1285I	CGRYCG	1 cut(s)	198
BshFI	GGCC	4 cut(s)	54, 97, 756, 1434
BsiEI	CGRYCG	1 cut(s)	198
BsiHKAI	GWGCWC	2 cut(s)	122, 619
BsiSI	CCGG	5 cut(s)	22, 46, 194, 303, 440
BslI	CCNNNNNNNGG	5 cut(s)	69, 104, 105, 647, 763
BsmAI	GTCTC	3 cut(s)	479, 1046, 1510
BsmI	GAATGC	3 cut(s)	554, 801, 1015
BsnI	GGCC	4 cut(s)	54, 97, 756, 1434
Bso31I	GGTCTC	1 cut(s)	1510
Bsp119I	TTCGAA	1 cut(s)	397
Bsp120I	GGGCCC	2 cut(s)	52, 95
Bsp1286I	GDGCHC	5 cut(s)	56, 99, 122, 619, 1189
Bsp1407I	TGTACA	1 cut(s)	1343
Bsp143I	GATC	6 cut(s)	320, 493, 1285, 1410, 1450, 1594
Bsp68I	TCGCGA	1 cut(s)	1249
BspACI	CCGC	6 cut(s)	92, 203, 244, 290, 757, 774
BspANI	GGCC	4 cut(s)	54, 97, 756, 1434
BspCNI	CTCAG	2 cut(s)	77, 1156
BspFNI	CGCG	1 cut(s)	1249
BspHI	TCATGA	2 cut(s)	744, 1447
BspLI	GGNNCC	6 cut(s)	54, 96, 97, 192, 1186, 1412
BspMAI	CTGCAG	2 cut(s)	1008, 1387
BspPI	GGATC	5 cut(s)	328, 501, 1405, 1418, 1602
BspT104I	TTCGAA	1 cut(s)	397
BspTI	CTTAAG	1 cut(s)	1498
BspTNI	GGTCTC	1 cut(s)	1510
BsrDI	GCAATG	7 cut(s)	32, 36, 507, 720, 732, 1275, 1356
BsrFI	RCCGGY	1 cut(s)	439
BsrGI	TGTACA	1 cut(s)	1343
BsrI	ACTGG	3 cut(s)	761, 1090, 1624
BssAI	RCCGGY	1 cut(s)	439
BssECI	CCNNGG	1 cut(s)	232
BssMI	GATC	6 cut(s)	320, 493, 1285, 1410, 1450, 1594
Bst2UI	CCWGG	1 cut(s)	233
Bst4CI	ACNGT	2 cut(s)	1038, 1612
BstAFI	CTTAAG	1 cut(s)	1498
BstAPI	GCANNNNNTGC	1 cut(s)	623
BstAUI	TGTACA	1 cut(s)	1343
BstBI	TTCGAA	1 cut(s)	397
BstC8I	GCNNGC	2 cut(s)	604, 890
BstDEI	CTNAG	4 cut(s)	64, 1051, 1143, 1397
BstF5I	GGATG	4 cut(s)	246, 628, 1061, 1073
BstFNI	CGCG	1 cut(s)	1249
BstH2I	RGCGCY	1 cut(s)	1214
BstHHI	GCGC	1 cut(s)	1213
BstKTI	GATC	6 cut(s)	323, 496, 1288, 1413, 1453, 1597
BstMAI	GTCTC	3 cut(s)	479, 1046, 1510
BstMBI	GATC	6 cut(s)	320, 493, 1285, 1410, 1450, 1594
BstMCI	CGRYCG	1 cut(s)	198
BstMWI	GCNNNNNNNGC	5 cut(s)	22, 623, 1019, 1028, 1358
BstNI	CCWGG	1 cut(s)	233
BstNSI	RCATGY	2 cut(s)	892, 988
BstPAI	GACNNNNGTC	1 cut(s)	234
BstSCI	CCNGG	1 cut(s)	231
BstSFI	CTRYAG	3 cut(s)	837, 1004, 1383
BstSLI	GKGCMC	3 cut(s)	56, 99, 619
BstUI	CGCG	1 cut(s)	1249
BstV1I	GCAGC	6 cut(s)	316, 682, 1000, 1043, 1397, 1516
BstX2I	RGATCY	1 cut(s)	1410
BstXI	CCANNNNNNTGG	2 cut(s)	1429, 1493
BstYI	RGATCY	1 cut(s)	1410
BsuRI	GGCC	4 cut(s)	54, 97, 756, 1434
BtrI	CACGTC	1 cut(s)	1506
BtsCI	GGATG	4 cut(s)	246, 628, 1061, 1073
BtsI	GCAGTG	1 cut(s)	597
BtsIMutI	CAGTG	4 cut(s)	597, 768, 1034, 1617
BtuMI	TCGCGA	1 cut(s)	1249
Cac8I	GCNNGC	2 cut(s)	604, 890
CciI	TCATGA	2 cut(s)	744, 1447
CfoI	GCGC	1 cut(s)	1213
Cfr10I	RCCGGY	1 cut(s)	439
Cfr13I	GGNCC	7 cut(s)	52, 53, 95, 96, 776, 1432, 1483
CseI	GACGC	3 cut(s)	837, 1108, 1377
Csp6I	GTAC	3 cut(s)	154, 207, 1344
CviAII	CATG	6 cut(s)	157, 745, 752, 889, 985, 1448
CviQI	GTAC	3 cut(s)	154, 207, 1344
DdeI	CTNAG	4 cut(s)	64, 1051, 1143, 1397
DpnI	GATC	6 cut(s)	322, 495, 1287, 1412, 1452, 1596
DpnII	GATC	6 cut(s)	320, 493, 1285, 1410, 1450, 1594
DriI	GACNNNNNGTC	1 cut(s)	1464
EaeI	YGGCCR	1 cut(s)	754
Eam1105I	GACNNNNNGTC	1 cut(s)	1464
Eco24I	GRGCYC	3 cut(s)	56, 99, 1189
Eco31I	GGTCTC	1 cut(s)	1510
Eco47I	GGWCC	2 cut(s)	776, 1483
Eco47III	AGCGCT	1 cut(s)	1212
Eco57I	CTGAAG	1 cut(s)	158
EcoO109I	RGGNCCY	1 cut(s)	95
EcoRI	GAATTC	1 cut(s)	460
EcoRII	CCWGG	1 cut(s)	231
EcoT22I	ATGCAT	1 cut(s)	1075
EcoT38I	GRGCYC	3 cut(s)	56, 99, 1189
FaeI	CATG	6 cut(s)	160, 748, 755, 892, 988, 1451
FatI	CATG	6 cut(s)	156, 744, 751, 888, 984, 1447
FauI	CCCGC	2 cut(s)	85, 196
FbaI	TGATCA	1 cut(s)	1450
FblI	GTMKAC	1 cut(s)	901
Fnu4HI	GCNGC	7 cut(s)	330, 696, 757, 1014, 1032, 1386, 1530
FokI	GGATG	4 cut(s)	253, 635, 1060, 1068
FriOI	GRGCYC	3 cut(s)	56, 99, 1189
Fsp4HI	GCNGC	7 cut(s)	330, 696, 757, 1014, 1032, 1386, 1530
FspBI	CTAG	8 cut(s)	384, 642, 858, 1023, 1292, 1328, 1436, 1590
GlaI	GCGC	1 cut(s)	1212
GluI	GCNGC	7 cut(s)	330, 696, 757, 1014, 1032, 1386, 1530
GsaI	CCCAGC	1 cut(s)	1365
GsuI	CTGGAG	2 cut(s)	136, 1182
HaeII	RGCGCY	1 cut(s)	1214
HaeIII	GGCC	4 cut(s)	54, 97, 756, 1434
HapII	CCGG	5 cut(s)	22, 46, 194, 303, 440
HgaI	GACGC	3 cut(s)	837, 1108, 1377
HhaI	GCGC	1 cut(s)	1213
Hin1II	CATG	6 cut(s)	160, 748, 755, 892, 988, 1451
Hin6I	GCGC	1 cut(s)	1211
HinP1I	GCGC	1 cut(s)	1211
HindIII	AAGCTT	2 cut(s)	14, 1416
HinfI	GANTC	9 cut(s)	128, 161, 212, 487, 741, 1199, 1244, 1255, 1550
HpaII	CCGG	5 cut(s)	22, 46, 194, 303, 440
HphI	GGTGA	2 cut(s)	446, 989
Hpy166II	GTNNAC	3 cut(s)	617, 902, 1091
Hpy188I	TCNGA	9 cut(s)	133, 217, 240, 853, 1046, 1069, 1377, 1510, 1572
Hpy8I	GTNNAC	3 cut(s)	617, 902, 1091
Hpy99I	CGWCG	3 cut(s)	224, 1393, 1510
HpyAV	CCTTC	7 cut(s)	133, 353, 420, 571, 916, 1129, 1548
HpyCH4III	ACNGT	2 cut(s)	1038, 1612
HpyCH4IV	ACGT	2 cut(s)	248, 1505
HpyF10VI	GCNNNNNNNGC	5 cut(s)	22, 623, 1019, 1028, 1358
HpyF3I	CTNAG	4 cut(s)	64, 1051, 1143, 1397
HpySE526I	ACGT	2 cut(s)	248, 1505
Hsp92II	CATG	6 cut(s)	160, 748, 755, 892, 988, 1451
HspAI	GCGC	1 cut(s)	1211
Ksp22I	TGATCA	1 cut(s)	1450
Kzo9I	GATC	6 cut(s)	320, 493, 1285, 1410, 1450, 1594
LmnI	GCTCC	7 cut(s)	35, 117, 133, 256, 409, 1163, 1184
Lsp1109I	GCAGC	6 cut(s)	316, 682, 1000, 1043, 1397, 1516
LweI	GCATC	6 cut(s)	231, 613, 1060, 1082, 1111, 1333
MaeI	CTAG	8 cut(s)	384, 642, 858, 1023, 1292, 1328, 1436, 1590
MaeII	ACGT	2 cut(s)	248, 1505
MaeIII	GTNAC	3 cut(s)	527, 941, 995
MalI	GATC	6 cut(s)	322, 495, 1287, 1412, 1452, 1596
MboI	GATC	6 cut(s)	320, 493, 1285, 1410, 1450, 1594
MboII	GAAGA	5 cut(s)	776, 797, 1097, 1125, 1596
MfeI	CAATTG	2 cut(s)	57, 957
MflI	RGATCY	1 cut(s)	1410
MhlI	GDGCHC	5 cut(s)	56, 99, 122, 619, 1189
MlyI	GAGTC	4 cut(s)	155, 481, 1249, 1253
MmeI	TCCRAC	2 cut(s)	218, 1488
Mph1103I	ATGCAT	1 cut(s)	1075
MseI	TTAA	3 cut(s)	947, 1499, 1666
MslI	CAYNNNNRTG	3 cut(s)	337, 725, 1229
MspA1I	CMGCKG	1 cut(s)	329
MspCI	CTTAAG	1 cut(s)	1498
MspI	CCGG	5 cut(s)	22, 46, 194, 303, 440
MspR9I	CCNGG	1 cut(s)	233
MunI	CAATTG	2 cut(s)	57, 957
Mva1269I	GAATGC	3 cut(s)	554, 801, 1015
MvaI	CCWGG	1 cut(s)	233
MvnI	CGCG	1 cut(s)	1249
MwoI	GCNNNNNNNGC	5 cut(s)	22, 623, 1019, 1028, 1358
NdeII	GATC	6 cut(s)	320, 493, 1285, 1410, 1450, 1594
NlaIII	CATG	6 cut(s)	160, 748, 755, 892, 988, 1451
NlaIV	GGNNCC	6 cut(s)	54, 96, 97, 192, 1186, 1412
NmuCI	GTSAC	3 cut(s)	527, 941, 995
NruI	TCGCGA	1 cut(s)	1249
NsiI	ATGCAT	1 cut(s)	1075
NspI	RCATGY	2 cut(s)	892, 988
NspV	TTCGAA	1 cut(s)	397
PaeI	GCATGC	1 cut(s)	892
PagI	TCATGA	2 cut(s)	744, 1447
PcsI	WCGNNNNNNNCGW	1 cut(s)	225
PctI	GAATGC	3 cut(s)	554, 801, 1015
PfeI	GAWTC	5 cut(s)	128, 212, 741, 1199, 1550
PkrI	GCNGC	7 cut(s)	331, 697, 758, 1015, 1033, 1387, 1531
PleI	GAGTC	4 cut(s)	155, 481, 1249, 1252
PpsI	GAGTC	4 cut(s)	155, 481, 1249, 1252
PshAI	GACNNNNGTC	1 cut(s)	234
Psp6I	CCWGG	1 cut(s)	231
PspFI	CCCAGC	1 cut(s)	1361
PspGI	CCWGG	1 cut(s)	231
PspN4I	GGNNCC	6 cut(s)	54, 96, 97, 192, 1186, 1412
PspOMI	GGGCCC	2 cut(s)	52, 95
PspPI	GGNCC	7 cut(s)	52, 53, 95, 96, 776, 1432, 1483
PstI	CTGCAG	2 cut(s)	1008, 1387
PsuI	RGATCY	1 cut(s)	1410
PvuII	CAGCTG	1 cut(s)	329
RruI	TCGCGA	1 cut(s)	1249
RsaI	GTAC	3 cut(s)	155, 208, 1345
RsaNI	GTAC	3 cut(s)	154, 207, 1344
RseI	CAYNNNNRTG	3 cut(s)	337, 725, 1229
SaqAI	TTAA	3 cut(s)	947, 1499, 1666
SatI	GCNGC	7 cut(s)	330, 696, 757, 1014, 1032, 1386, 1530
Sau3AI	GATC	6 cut(s)	320, 493, 1285, 1410, 1450, 1594
Sau96I	GGNCC	7 cut(s)	52, 53, 95, 96, 776, 1432, 1483
SchI	GAGTC	4 cut(s)	155, 481, 1249, 1253
ScrFI	CCNGG	1 cut(s)	233
SduI	GDGCHC	5 cut(s)	56, 99, 122, 619, 1189
SfaNI	GCATC	6 cut(s)	231, 613, 1060, 1082, 1111, 1333
SfcI	CTRYAG	3 cut(s)	837, 1004, 1383
SfuI	TTCGAA	1 cut(s)	397
SinI	GGWCC	2 cut(s)	776, 1483
SmiMI	CAYNNNNRTG	3 cut(s)	337, 725, 1229
SmlI	CTYRAG	4 cut(s)	11, 167, 935, 1498
SmoI	CTYRAG	4 cut(s)	11, 167, 935, 1498
SphI	GCATGC	1 cut(s)	892
SsiI	CCGC	6 cut(s)	92, 203, 244, 290, 757, 774
SspI	AATATT	1 cut(s)	1173
SspMI	CTAG	8 cut(s)	384, 642, 858, 1023, 1292, 1328, 1436, 1590
StyD4I	CCNGG	1 cut(s)	231
TaaI	ACNGT	2 cut(s)	1038, 1612
TaiI	ACGT	2 cut(s)	251, 1508
TaqI	TCGA	3 cut(s)	228, 397, 1133
TaqII	GACCGA	1 cut(s)	652
TatI	WGTACW	2 cut(s)	153, 1343
TauI	GCSGC	1 cut(s)	759
TfiI	GAWTC	5 cut(s)	128, 212, 741, 1199, 1550
Tru1I	TTAA	3 cut(s)	947, 1499, 1666
Tru9I	TTAA	3 cut(s)	947, 1499, 1666
TscAI	CASTG	4 cut(s)	597, 768, 1041, 1624
TseFI	GTSAC	3 cut(s)	527, 941, 995
TseI	GCWGC	6 cut(s)	329, 695, 1013, 1031, 1385, 1529
Tsp45I	GTSAC	3 cut(s)	527, 941, 995
TspDTI	ATGAA	7 cut(s)	17, 284, 518, 761, 1436, 1530, 1629
TspGWI	ACGGA	3 cut(s)	22, 224, 422
TspRI	CASTG	4 cut(s)	597, 768, 1041, 1624
Vha464I	CTTAAG	1 cut(s)	1498
VneI	GTGCAC	1 cut(s)	615
VpaK11BI	GGWCC	2 cut(s)	776, 1483
XapI	RAATTY	3 cut(s)	6, 460, 1062
XbaI	TCTAGA	1 cut(s)	1327
XceI	RCATGY	2 cut(s)	892, 988
XmiI	GTMKAC	1 cut(s)	901
XspI	CTAG	8 cut(s)	384, 642, 858, 1023, 1292, 1328, 1436, 1590
Zsp2I	ATGCAT	1 cut(s)	1075

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.