MD07G1311100.v1.1

Folate-Biopterin Transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0011273
Physical Location & Seq
Reverse (-)
36606360 .. 36609477
3118 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1311100.v1.1.491

Sequence Viewer

Length: 1323 bp
ATGCTAGAGCCATCGTCGGAGGGTGACGCGGACAAGATCCGGAAGTTGGTGGGGTTGGGGTACTGGGTGCAGGGCTTCAGGTGCTTCCCATGGATGGCCGTCAACTTCTTCCTCAAGGACGGCCTCCACGTGGACCCCTCCACTCTCCAGCTCCTGCAGAACTCCGCCAACCTCCCCATGGTCGCCAAGCCCCTTTACGGTGTCGTTTCAGACGCCGTCTACATTGCTGGCCAGCACCGTATTCCCTATATTGCCCTTGGAGCGGTCTTACAAGCAGTGTCATGGCTAGCAATAGCAATGCTCTCTGAATCAGGCATCTCAATTGGAACCATAACACTATATCTCCTCCTCAGTAATCTCGGTGCTTCAATTGCTGAGGTAGCCAATGATGCCATTGTTGCAGAGGCAGGAAAACAGTCTACTTCCCCAAAAAATTCCCAACCGTCATCCTCAGGCGAGCTTCAATCATTTTCTTCGATGGCTTCAGCTATGGGTGGAGTCCTTGGATGTCTCCTAGGTGGTCTTGCCATTGACAGATTATCTCCCCAAAAGATGTTCCTGTTCTATGGCCTTCTCCTCACTGTCCACTTTTTGATAAGCATTTTTGTCCAGGAAAGCTCTTTGAACCATACCGAAGGAACAGCCAATGTTGGGATTCGAGAGAAACTTTTTGAGCTCTTGGTTGCTTTAAGAGAACCTGAGATCACTTACTCAATATCATGGTTTGCGGCATCTTATGCCATTATTCCAGCATTGACAGGCACCATGTTCTTTTACCAGGCACAGTACTTGAATATTGACTCATCTCTGTTGGGAATCTCAAAGGTGTTTGGCCAAGTGGCAATGCTTCTATGGAGTGTCATTTATAATCGTCACTTGAAGTCAGTCAGACCTAGGAAACTAGTTTCTGCCATTCAGGTTACAATGGCTATATTTATGATTTCTGATGCGTTGTTTGTAAATAGGATTTATCAAAATTACATGGGGGTGCCAGACAATTTCTACGTCGTGGTGTTTTCTGGCATTTCGGAGGTTCTCTTGTTTATGAAGATTTTGCCCTTCAGCATTCTTATAGCCAAACTCTGCCCACGAGGATGCGAGGGATCTCTAATGGCGTTTGCGATGTCTGCAATAGCGGTTGCATTCATAGTAAGTGGATATCTGGGTGTTGTGCTTGCATCGTATGTTGGGGTCACGGGAAATGACTTTTCAGGGTTTCCACTTGCCCTACTAATACAAGCACTGCTCACAGTGTTGCCACTATATTGGTCATCGTGTATCCCTGATGATGTAAAATCAAAATCCCGTCAGAAGGAGGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BT1

Protein Analysis

441

Amino Acids

47.83

Weight (kDa)

6.53

Isoelectric Point (pI)

35.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BT1 PF03092 24 - 431 9.4e-111 BT1 family
MFS_1 PF07690 32 - 381 2.2e-13 Major Facilitator Superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011273)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G64890
fragaria_vesca FvH4_7g34292
malus_domestica MD07G1311100.v1.1
prunus_persica Prupe.2G330100_v2.0.a1
pyrus_communis pycom07g28340
rosa_chinensis RchiOBHm_Chr1g0384681
rosa_laevigata RLG00000026016
rosa_multiflora Rmu_sc0012136.1_g000022
rosa_roxburghii Rroxscaffold_4G00276470 Rroxscaffold_7G00218000
rosa_rugosa Rorug01G0450700 Rorug01G0450700 Rorug01G0450800
rosa_samantha Rh1AG475200 Rh1BG433400 Rh1CG446800 Rh1CG446900 Rh1DG465900 Rh6BG010000
rosa_wichuraiana Rw1G039740

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 867
AccB1I GGYRCC 2 cut(s) 761, 988
AccBSI CCGCTC 1 cut(s) 263
AccI GTMKAC 2 cut(s) 219, 419
AccII CGCG 1 cut(s) 29
AccIII TCCGGA 1 cut(s) 39
AciI CCGC 5 cut(s) 29, 165, 263, 728, 1136
AclWI GGATC 2 cut(s) 31, 1111
AcoI YGGCCR 3 cut(s) 96, 229, 832
AcsI RAATTY 1 cut(s) 433
AcuI CTGAAG 3 cut(s) 61, 468, 1045
AcvI CACGTG 1 cut(s) 130
AcyI GRCGYC 1 cut(s) 213
AfaI GTAC 2 cut(s) 62, 788
AfiI CCNNNNNNNGG 8 cut(s) 46, 94, 130, 178, 197, 262, 651, 1312
AgsI TTSAA 5 cut(s) 369, 464, 625, 793, 880
AhlI ACTAGT 1 cut(s) 901
AjnI CCWGG 2 cut(s) 609, 777
AluBI AGCT 5 cut(s) 151, 460, 488, 618, 676
AluI AGCT 5 cut(s) 151, 460, 488, 618, 676
Alw21I GWGCWC 1 cut(s) 678
Alw26I GTCTC 1 cut(s) 515
AlwI GGATC 2 cut(s) 31, 1111
AlwNI CAGNNNCTG 1 cut(s) 154
Aor13HI TCCGGA 1 cut(s) 39
AoxI GGCC 5 cut(s) 96, 121, 229, 568, 832
ApoI RAATTY 1 cut(s) 433
AspA2I CCTAGG 2 cut(s) 514, 893
AspS9I GGNCC 1 cut(s) 133
AsuHPI GGTGA 1 cut(s) 35
AsuNHI GCTAGC 1 cut(s) 286
AvaII GGWCC 1 cut(s) 133
AvrII CCTAGG 2 cut(s) 514, 893
AxyI CCTNAGG 1 cut(s) 451
BalI TGGCCA 2 cut(s) 231, 834
BanI GGYRCC 2 cut(s) 761, 988
BanII GRGCYC 1 cut(s) 678
BauI CACGAG 1 cut(s) 1089
BbrPI CACGTG 1 cut(s) 130
Bbv12I GWGCWC 1 cut(s) 678
BbvCI CCTCAGC 1 cut(s) 375
BccI CCATC 3 cut(s) 19, 88, 472
BceAI ACGGC 3 cut(s) 83, 136, 200
BciT130I CCWGG 2 cut(s) 611, 779
BciVI GTATCC 1 cut(s) 1289
BcoDI GTCTC 1 cut(s) 515
BcuI ACTAGT 1 cut(s) 901
BfaI CTAG 5 cut(s) 5, 287, 515, 894, 902
BfmI CTRYAG 1 cut(s) 155
BfuI GTATCC 1 cut(s) 1289
BisI GCNGC 1 cut(s) 729
BlnI CCTAGG 2 cut(s) 514, 893
BlsI GCNGC 1 cut(s) 730
BmcAI AGTACT 1 cut(s) 788
Bme1390I CCNGG 2 cut(s) 611, 779
Bme18I GGWCC 1 cut(s) 133
BmgT120I GGNCC 1 cut(s) 133
BmiI GGNNCC 4 cut(s) 135, 328, 763, 990
BmrFI CCNGG 2 cut(s) 611, 779
BmrI ACTGGG 1 cut(s) 73
BmsI GCATC 6 cut(s) 324, 379, 740, 937, 1085, 1187
BmtI GCTAGC 1 cut(s) 290
BmuI ACTGGG 1 cut(s) 73
BpmI CTGGAG 1 cut(s) 131
Bpu10I CCTNAGC 1 cut(s) 375
BpuEI CTTGAG 1 cut(s) 98
BsaAI YACGTR 1 cut(s) 130
BsaHI GRCGYC 1 cut(s) 213
BsaJI CCNNGG 6 cut(s) 89, 177, 256, 502, 514, 893
BsaWI WCCGGW 1 cut(s) 39
BsaXI ACNNNNNCTCC 2 cut(s) 327, 357
Bsc4I CCNNNNNNNGG 8 cut(s) 46, 94, 130, 178, 197, 262, 651, 1312
Bse1I ACTGG 1 cut(s) 68
Bse21I CCTNAGG 1 cut(s) 451
Bse3DI GCAATG 3 cut(s) 222, 303, 849
BseAI TCCGGA 1 cut(s) 39
BseBI CCWGG 2 cut(s) 611, 779
BseDI CCNNGG 6 cut(s) 89, 177, 256, 502, 514, 893
BseGI GGATG 4 cut(s) 99, 446, 512, 1100
BseLI CCNNNNNNNGG 8 cut(s) 46, 94, 130, 178, 197, 262, 651, 1312
BseMI GCAATG 3 cut(s) 222, 303, 849
BseMII CTCAG 4 cut(s) 364, 366, 465, 690
BseNI ACTGG 1 cut(s) 68
BseRI GAGGAG 3 cut(s) 335, 338, 566
BsgI GTGCAG 1 cut(s) 89
Bsh1236I CGCG 1 cut(s) 29
BshFI GGCC 5 cut(s) 98, 123, 231, 570, 834
BshNI GGYRCC 2 cut(s) 761, 988
BsiHKAI GWGCWC 1 cut(s) 678
BsiSI CCGG 1 cut(s) 40
BslI CCNNNNNNNGG 8 cut(s) 46, 94, 130, 178, 197, 262, 651, 1312
BsmAI GTCTC 1 cut(s) 515
BsmI GAATGC 2 cut(s) 1065, 1142
BsnI GGCC 5 cut(s) 98, 123, 231, 570, 834
Bsp1286I GDGCHC 1 cut(s) 678
Bsp13I TCCGGA 1 cut(s) 39
Bsp143I GATC 3 cut(s) 36, 702, 1103
Bsp19I CCATGG 2 cut(s) 89, 177
BspACI CCGC 5 cut(s) 29, 165, 263, 728, 1136
BspANI GGCC 5 cut(s) 98, 123, 231, 570, 834
BspCNI CTCAG 4 cut(s) 363, 367, 464, 691
BspEI TCCGGA 1 cut(s) 39
BspFNI CGCG 1 cut(s) 29
BspLI GGNNCC 4 cut(s) 135, 328, 763, 990
BspMAI CTGCAG 1 cut(s) 159
BspOI GCTAGC 1 cut(s) 290
BspPI GGATC 2 cut(s) 31, 1111
BspT107I GGYRCC 2 cut(s) 761, 988
BsrBI CCGCTC 1 cut(s) 263
BsrDI GCAATG 3 cut(s) 222, 303, 849
BsrI ACTGG 1 cut(s) 68
BssECI CCNNGG 6 cut(s) 89, 177, 256, 502, 514, 893
BssMI GATC 3 cut(s) 36, 702, 1103
BssNI GRCGYC 1 cut(s) 213
BssSI CACGAG 1 cut(s) 1089
BssT1I CCWWGG 6 cut(s) 89, 177, 256, 502, 514, 893
Bst2BI CACGAG 1 cut(s) 1089
Bst2UI CCWGG 2 cut(s) 611, 779
Bst4CI ACNGT 7 cut(s) 200, 239, 417, 444, 583, 786, 1252
BstACI GRCGYC 1 cut(s) 213
BstAPI GCANNNNNTGC 1 cut(s) 737
BstBAI YACGTR 1 cut(s) 130
BstC8I GCNNGC 5 cut(s) 229, 233, 288, 458, 1176
BstDEI CTNAG 4 cut(s) 350, 375, 451, 699
BstDSI CCRYGG 2 cut(s) 89, 177
BstF5I GGATG 4 cut(s) 99, 446, 512, 1100
BstFNI CGCG 1 cut(s) 29
BstKTI GATC 3 cut(s) 39, 705, 1106
BstMAI GTCTC 1 cut(s) 515
BstMBI GATC 3 cut(s) 36, 702, 1103
BstMWI GCNNNNNNNGC 8 cut(s) 81, 260, 371, 380, 389, 398, 737, 1127
BstNI CCWGG 2 cut(s) 611, 779
BstSCI CCNGG 2 cut(s) 609, 777
BstSFI CTRYAG 1 cut(s) 155
BstUI CGCG 1 cut(s) 29
BstX2I RGATCY 2 cut(s) 36, 1103
BstXI CCANNNNNNTGG 1 cut(s) 1266
BstYI RGATCY 2 cut(s) 36, 1103
Bsu36I CCTNAGG 1 cut(s) 451
BsuI GTATCC 1 cut(s) 1289
BsuRI GGCC 5 cut(s) 98, 123, 231, 570, 834
BtgI CCRYGG 2 cut(s) 89, 177
BtgZI GCGATG 1 cut(s) 1136
BtsCI GGATG 4 cut(s) 99, 446, 512, 1100
BtsI GCAGTG 2 cut(s) 282, 1241
BtsIMutI CAGTG 4 cut(s) 282, 579, 1241, 1257
Cac8I GCNNGC 5 cut(s) 229, 233, 288, 458, 1176
CaiI CAGNNNCTG 1 cut(s) 154
Cfr13I GGNCC 1 cut(s) 133
CseI GACGC 2 cut(s) 35, 221
Csp6I GTAC 2 cut(s) 61, 787
CviAII CATG 6 cut(s) 90, 178, 282, 720, 766, 982
CviQI GTAC 2 cut(s) 61, 787
DdeI CTNAG 4 cut(s) 350, 375, 451, 699
DpnI GATC 3 cut(s) 38, 704, 1105
DpnII GATC 3 cut(s) 36, 702, 1103
EaeI YGGCCR 3 cut(s) 96, 229, 832
EciI GGCGGA 1 cut(s) 154
Ecl136II GAGCTC 1 cut(s) 676
Eco130I CCWWGG 6 cut(s) 89, 177, 256, 502, 514, 893
Eco24I GRGCYC 1 cut(s) 678
Eco32I GATATC 1 cut(s) 1160
Eco47I GGWCC 1 cut(s) 133
Eco53kI GAGCTC 1 cut(s) 676
Eco57I CTGAAG 3 cut(s) 61, 468, 1045
Eco72I CACGTG 1 cut(s) 130
Eco81I CCTNAGG 1 cut(s) 451
EcoICRI GAGCTC 1 cut(s) 676
EcoRII CCWGG 2 cut(s) 609, 777
EcoRV GATATC 1 cut(s) 1160
EcoT14I CCWWGG 6 cut(s) 89, 177, 256, 502, 514, 893
EcoT38I GRGCYC 1 cut(s) 678
ErhI CCWWGG 6 cut(s) 89, 177, 256, 502, 514, 893
FaeI CATG 6 cut(s) 93, 181, 285, 723, 769, 985
FatI CATG 6 cut(s) 89, 177, 281, 719, 765, 981
FblI GTMKAC 2 cut(s) 219, 419
Fnu4HI GCNGC 1 cut(s) 729
FokI GGATG 4 cut(s) 106, 433, 519, 1107
FriOI GRGCYC 1 cut(s) 678
Fsp4HI GCNGC 1 cut(s) 729
FspBI CTAG 5 cut(s) 5, 287, 515, 894, 902
GluI GCNGC 1 cut(s) 729
GsuI CTGGAG 1 cut(s) 131
HaeIII GGCC 5 cut(s) 98, 123, 231, 570, 834
HapII CCGG 1 cut(s) 40
HgaI GACGC 2 cut(s) 35, 221
Hin1I GRCGYC 1 cut(s) 213
Hin1II CATG 6 cut(s) 93, 181, 285, 723, 769, 985
HincII GTYRAC 1 cut(s) 103
HindII GTYRAC 1 cut(s) 103
HinfI GANTC 5 cut(s) 308, 498, 655, 800, 816
HpaII CCGG 1 cut(s) 40
HphI GGTGA 1 cut(s) 35
Hpy166II GTNNAC 5 cut(s) 103, 133, 220, 420, 586
Hpy188I TCNGA 7 cut(s) 19, 211, 307, 890, 946, 1030, 1311
Hpy188III TCNNGA 2 cut(s) 40, 659
Hpy8I GTNNAC 5 cut(s) 103, 133, 220, 420, 586
Hpy99I CGWCG 2 cut(s) 19, 1010
HpyAV CCTTC 4 cut(s) 581, 629, 1069, 1306
HpyCH4III ACNGT 7 cut(s) 200, 239, 417, 444, 583, 786, 1252
HpyCH4IV ACGT 2 cut(s) 129, 1005
HpyCH4V TGCA 6 cut(s) 70, 157, 401, 1130, 1142, 1178
HpyF10VI GCNNNNNNNGC 8 cut(s) 81, 260, 371, 380, 389, 398, 737, 1127
HpyF3I CTNAG 4 cut(s) 350, 375, 451, 699
HpySE526I ACGT 2 cut(s) 129, 1005
Hsp92I GRCGYC 1 cut(s) 213
Hsp92II CATG 6 cut(s) 93, 181, 285, 723, 769, 985
Kpn2I TCCGGA 1 cut(s) 39
Kzo9I GATC 3 cut(s) 36, 702, 1103
LmnI GCTCC 2 cut(s) 156, 260
LweI GCATC 6 cut(s) 324, 379, 740, 937, 1085, 1187
MaeI CTAG 5 cut(s) 5, 287, 515, 894, 902
MaeII ACGT 2 cut(s) 129, 1005
MaeIII GTNAC 4 cut(s) 23, 872, 919, 1192
MalI GATC 3 cut(s) 38, 704, 1105
MbiI CCGCTC 1 cut(s) 263
MboI GATC 3 cut(s) 36, 702, 1103
MboII GAAGA 3 cut(s) 100, 465, 1060
MfeI CAATTG 2 cut(s) 321, 369
MflI RGATCY 2 cut(s) 36, 1103
MhlI GDGCHC 1 cut(s) 678
MlsI TGGCCA 2 cut(s) 231, 834
MluCI AATT 5 cut(s) 321, 369, 433, 976, 997
MluNI TGGCCA 2 cut(s) 231, 834
MlyI GAGTC 2 cut(s) 507, 794
Mox20I TGGCCA 2 cut(s) 231, 834
MroI TCCGGA 1 cut(s) 39
MscI TGGCCA 2 cut(s) 231, 834
MseI TTAA 1 cut(s) 689
MslI CAYNNNNRTG 2 cut(s) 986, 1093
Msp20I TGGCCA 2 cut(s) 231, 834
MspI CCGG 1 cut(s) 40
MspR9I CCNGG 2 cut(s) 611, 779
MunI CAATTG 2 cut(s) 321, 369
Mva1269I GAATGC 2 cut(s) 1065, 1142
MvaI CCWGG 2 cut(s) 611, 779
MvnI CGCG 1 cut(s) 29
MwoI GCNNNNNNNGC 8 cut(s) 81, 260, 371, 380, 389, 398, 737, 1127
NcoI CCATGG 2 cut(s) 89, 177
NdeII GATC 3 cut(s) 36, 702, 1103
NheI GCTAGC 1 cut(s) 286
NlaIII CATG 6 cut(s) 93, 181, 285, 723, 769, 985
NlaIV GGNNCC 4 cut(s) 135, 328, 763, 990
NmuCI GTSAC 3 cut(s) 23, 872, 1192
PcsI WCGNNNNNNNCGW 1 cut(s) 126
PctI GAATGC 2 cut(s) 1065, 1142
PfeI GAWTC 3 cut(s) 308, 655, 816
PflFI GACNNNGTC 1 cut(s) 215
PfoI TCCNGGA 1 cut(s) 609
PkrI GCNGC 1 cut(s) 730
PleI GAGTC 2 cut(s) 506, 794
PmaCI CACGTG 1 cut(s) 130
PmlI CACGTG 1 cut(s) 130
PpsI GAGTC 2 cut(s) 506, 794
Ppu21I YACGTR 1 cut(s) 130
PsiI TTATAA 1 cut(s) 867
Psp124BI GAGCTC 1 cut(s) 678
Psp6I CCWGG 2 cut(s) 609, 777
PspCI CACGTG 1 cut(s) 130
PspGI CCWGG 2 cut(s) 609, 777
PspN4I GGNNCC 4 cut(s) 135, 328, 763, 990
PspPI GGNCC 1 cut(s) 133
PstI CTGCAG 1 cut(s) 159
PstNI CAGNNNCTG 1 cut(s) 154
PsuI RGATCY 2 cut(s) 36, 1103
PsyI GACNNNGTC 1 cut(s) 215
RsaI GTAC 2 cut(s) 62, 788
RsaNI GTAC 2 cut(s) 61, 787
RseI CAYNNNNRTG 2 cut(s) 986, 1093
SacI GAGCTC 1 cut(s) 678
SaqAI TTAA 1 cut(s) 689
SatI GCNGC 1 cut(s) 729
Sau3AI GATC 3 cut(s) 36, 702, 1103
Sau96I GGNCC 1 cut(s) 133
ScaI AGTACT 1 cut(s) 788
SchI GAGTC 2 cut(s) 507, 794
ScrFI CCNGG 2 cut(s) 611, 779
SduI GDGCHC 1 cut(s) 678
SfaNI GCATC 6 cut(s) 324, 379, 740, 937, 1085, 1187
SfcI CTRYAG 1 cut(s) 155
SinI GGWCC 1 cut(s) 133
SmiMI CAYNNNNRTG 2 cut(s) 986, 1093
SmlI CTYRAG 1 cut(s) 113
SmoI CTYRAG 1 cut(s) 113
SpeI ACTAGT 1 cut(s) 901
Sse9I AATT 5 cut(s) 321, 369, 433, 976, 997
SsiI CCGC 5 cut(s) 29, 165, 263, 728, 1136
SspI AATATT 1 cut(s) 796
SspMI CTAG 5 cut(s) 5, 287, 515, 894, 902
SstI GAGCTC 1 cut(s) 678
StyD4I CCNGG 2 cut(s) 609, 777
StyI CCWWGG 6 cut(s) 89, 177, 256, 502, 514, 893
TaaI ACNGT 7 cut(s) 200, 239, 417, 444, 583, 786, 1252
TaiI ACGT 2 cut(s) 132, 1008
TaqI TCGA 2 cut(s) 476, 658
TasI AATT 5 cut(s) 321, 369, 433, 976, 997
TatI WGTACW 1 cut(s) 786
TauI GCSGC 1 cut(s) 731
TfiI GAWTC 3 cut(s) 308, 655, 816
Tru1I TTAA 1 cut(s) 689
Tru9I TTAA 1 cut(s) 689
TscAI CASTG 4 cut(s) 282, 586, 1248, 1257
TseFI GTSAC 3 cut(s) 23, 872, 1192
Tsp45I GTSAC 3 cut(s) 23, 872, 1192
TspDTI ATGAA 2 cut(s) 1061, 1135
TspRI CASTG 4 cut(s) 282, 586, 1248, 1257
Tth111I GACNNNGTC 1 cut(s) 215
VpaK11BI GGWCC 1 cut(s) 133
XapI RAATTY 1 cut(s) 433
XcmI CCANNNNNNNNNTGG 1 cut(s) 175
XmaJI CCTAGG 2 cut(s) 514, 893
XmiI GTMKAC 2 cut(s) 219, 419
XspI CTAG 5 cut(s) 5, 287, 515, 894, 902
ZrmI AGTACT 1 cut(s) 788
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.