Rh1AG475200

Folate-Biopterin Transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1A
OG0011273
Physical Location & Seq
Forward (+)
68591542 .. 68594754
3213 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1AG475200.1

Sequence Viewer

Length: 1329 bp
ATGGTATTGGTGACGTCGTCGGAGGCGAAGATCCGGAAGGTGTTGGGATTGGGATACTGGGTGCAGGGCTTCAGGTGCTTCCCATGGTTGGCCGTCAACTTCTTCCTTAAGGACGGACTCCAAGTGGACTCCTCCACTCTCCAGCTCCTCCAGAACTCCGCCAACCTACCCATGGTCGGAAAGCCCCTTTACGGTCTCGTTTCCGACGCCATCTACATTGCTGGCCAGCACCGCATTCCCTATCTCGCCCTTGGTGCGTTCTTACAAGCAGTGTCATGGTTAGCTATAGCAATCCTCTCCCAATCAGGCATCTCAATTAGCGCTATGACCCTATATCTCCTCCTCAGTAATTTGGGTGCTTCAATTGCTGAGGTTGCCAATGATGCCATTGTTGCAGAGGTAGGAAAACAGCCTGCTTCCTCCAAAAGTCCACAACCATCATCCTCGGGCGAGCTTCAGTCTTTTGTTTGGATGTCTTCAGCAGTGGGTGGAATCCTAGGATTTCTCCTAGGTGGTATTGCCATCGACAGATATGCTCCCCAAAAGATGTTCCTGTTCTACGGCTTTCTCCTCGGTGTTCAGTTTTTCATTAGCATTCTTGTCAAGGAGAGCTCTATGAACCTTCCCAAGAGAAGATCTGATGTTGGAATTCGAGAGAAGCTTTCCGAGCTGTCAATTGCATTAAAAAAACCTGAGATTGCTTACTCAATTACATGGTTTGCAGCATCATATGCCATCATTCCATCATTGACAGGCACGATGTTCTTTTACCAGACAGAATATTTGAATATTGACTCATCGGTGTTGGGAATCTCAAAGGTGTTTGGCCAGGCGGCAATGCTTCTATGGAGTGTCATCTATAATCGTCACCTGAAACCGGTAACACCGAGGAAACTAATTTCAGCCATTCAGGTTACCATGGCTTTATTTATGATTTCTGATGCGTTGTTTGCAAATGGGTTTTACCGGAATATGGGGCTGTCAGACAATTTTTACATCGTAATATTTTCTGGCCTCTCAGAGGTTCTCTTGTTTATTAAGATTCTGCCCTTCAGCATTCTTGTAGCCAAGCTCTGCCCACCGGGATGCGAGGGATCTCTAATGGCATTTGTAATGTCTGCAATAGCAGTTGCGTTGATTATGAGCGGATATCTAGGTATTGCGCTTGCATCTTATGTTGGGATCACAGGAAATGATTTTTCAGGGCTTCCGCTTGCCTTCCTAATACAAGCATTTCTCACACTCTTGCCACTCTATTGGTCATCATGTATCCCTATCCTTGAATCGGAACCCGATCATGTAAAAGCAAAACCCAGTAAGAAGGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BT1

Protein Analysis

442

Amino Acids

48.08

Weight (kDa)

8.95

Isoelectric Point (pI)

40.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BT1 PF03092 22 - 434 9.5e-113 BT1 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011273)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G64890
fragaria_vesca FvH4_7g34292
malus_domestica MD07G1311100.v1.1
prunus_persica Prupe.2G330100_v2.0.a1
pyrus_communis pycom07g28340
rosa_chinensis RchiOBHm_Chr1g0384681
rosa_laevigata RLG00000026016
rosa_multiflora Rmu_sc0012136.1_g000022
rosa_roxburghii Rroxscaffold_4G00276470 Rroxscaffold_7G00218000
rosa_rugosa Rorug01G0450700 Rorug01G0450700 Rorug01G0450800
rosa_samantha Rh1AG475200 Rh1BG433400 Rh1CG446800 Rh1CG446900 Rh1DG465900 Rh6BG010000
rosa_wichuraiana Rw1G039740

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 17
AccBSI CCGCTC 1 cut(s) 1146
AccIII TCCGGA 1 cut(s) 33
AciI CCGC 5 cut(s) 159, 232, 833, 1146, 1211
AclWI GGATC 3 cut(s) 25, 1102, 1190
AcoI YGGCCR 3 cut(s) 90, 223, 826
AcsI RAATTY 1 cut(s) 648
AcuI CTGAAG 4 cut(s) 55, 440, 462, 1036
AcyI GRCGYC 2 cut(s) 14, 207
AfeI AGCGCT 1 cut(s) 322
AfiI CCNNNNNNNGG 8 cut(s) 88, 172, 176, 191, 877, 973, 1021, 1285
AflII CTTAAG 1 cut(s) 107
AgeI ACCGGT 1 cut(s) 877
AgsI TTSAA 3 cut(s) 363, 787, 1283
AjnI CCWGG 1 cut(s) 828
AluBI AGCT 7 cut(s) 145, 284, 454, 612, 661, 670, 1072
AluI AGCT 7 cut(s) 145, 284, 454, 612, 661, 670, 1072
Alw21I GWGCWC 1 cut(s) 614
Alw26I GTCTC 1 cut(s) 200
AlwI GGATC 3 cut(s) 25, 1102, 1190
Ama87I CYCGRG 1 cut(s) 445
Aor13HI TCCGGA 1 cut(s) 33
Aor51HI AGCGCT 1 cut(s) 322
AoxI GGCC 4 cut(s) 90, 223, 826, 1012
ApeKI GCWGC 1 cut(s) 722
ApoI RAATTY 1 cut(s) 648
ArsI GACNNNNNNTTYG 2 cut(s) 766, 798
AsiGI ACCGGT 1 cut(s) 877
AspA2I CCTAGG 2 cut(s) 496, 508
AspLEI GCGC 2 cut(s) 323, 1165
AsuC2I CCSGG 1 cut(s) 1083
AsuHPI GGTGA 2 cut(s) 22, 860
AvaI CYCGRG 1 cut(s) 445
AvrII CCTAGG 2 cut(s) 496, 508
BalI TGGCCA 2 cut(s) 225, 828
BanII GRGCYC 1 cut(s) 614
BbsI GAAGAC 1 cut(s) 468
Bbv12I GWGCWC 1 cut(s) 614
BbvCI CCTCAGC 1 cut(s) 369
BbvI GCAGC 1 cut(s) 734
BccI CCATC 5 cut(s) 218, 445, 530, 743, 751
BceAI ACGGC 2 cut(s) 77, 577
BcgI CGANNNNNNTGC 2 cut(s) 515, 549
BciT130I CCWGG 1 cut(s) 830
BciVI GTATCC 2 cut(s) 47, 1280
BcnI CCSGG 1 cut(s) 1083
BcoDI GTCTC 1 cut(s) 200
BfaI CTAG 3 cut(s) 497, 509, 1154
BfmI CTRYAG 1 cut(s) 285
BfoI RGCGCY 1 cut(s) 324
BfrI CTTAAG 1 cut(s) 107
BfuI GTATCC 2 cut(s) 47, 1280
BglII AGATCT 1 cut(s) 635
BisI GCNGC 2 cut(s) 723, 834
BlnI CCTAGG 2 cut(s) 496, 508
BlsI GCNGC 2 cut(s) 724, 835
Bme1390I CCNGG 2 cut(s) 830, 1083
BmeT110I CYCGRG 1 cut(s) 445
BmiI GGNNCC 1 cut(s) 1290
BmrFI CCNGG 2 cut(s) 830, 1083
BmrI ACTGGG 2 cut(s) 67, 1308
BmsI GCATC 6 cut(s) 318, 373, 734, 931, 1076, 1178
BmuI ACTGGG 2 cut(s) 67, 1308
BpiI GAAGAC 1 cut(s) 468
BpmI CTGGAG 2 cut(s) 125, 134
Bpu10I CCTNAGC 1 cut(s) 369
BpuMI CCSGG 1 cut(s) 1083
BsaHI GRCGYC 2 cut(s) 14, 207
BsaI GGTCTC 1 cut(s) 200
BsaJI CCNNGG 9 cut(s) 83, 171, 250, 444, 496, 508, 571, 887, 918
BsaWI WCCGGW 3 cut(s) 33, 877, 966
Bsc4I CCNNNNNNNGG 8 cut(s) 88, 172, 176, 191, 877, 973, 1021, 1285
Bse118I RCCGGY 1 cut(s) 877
Bse1I ACTGG 2 cut(s) 62, 1314
Bse3DI GCAATG 2 cut(s) 216, 843
BseAI TCCGGA 1 cut(s) 33
BseBI CCWGG 1 cut(s) 830
BseDI CCNNGG 9 cut(s) 83, 171, 250, 444, 496, 508, 571, 887, 918
BseGI GGATG 3 cut(s) 440, 477, 1091
BseLI CCNNNNNNNGG 8 cut(s) 88, 172, 176, 191, 877, 973, 1021, 1285
BseMI GCAATG 2 cut(s) 216, 843
BseMII CTCAG 4 cut(s) 358, 360, 684, 1032
BseNI ACTGG 2 cut(s) 62, 1314
BseRI GAGGAG 5 cut(s) 121, 137, 329, 332, 560
BseXI GCAGC 1 cut(s) 734
BsgI GTGCAG 1 cut(s) 83
BshFI GGCC 4 cut(s) 92, 225, 828, 1014
BshTI ACCGGT 1 cut(s) 877
BsiHKAI GWGCWC 1 cut(s) 614
BsiHKCI CYCGRG 1 cut(s) 445
BsiSI CCGG 4 cut(s) 34, 878, 967, 1082
BslI CCNNNNNNNGG 8 cut(s) 88, 172, 176, 191, 877, 973, 1021, 1285
BsmAI GTCTC 1 cut(s) 200
BsmI GAATGC 3 cut(s) 234, 594, 1056
BsnI GGCC 4 cut(s) 92, 225, 828, 1014
Bso31I GGTCTC 1 cut(s) 200
BsoBI CYCGRG 1 cut(s) 445
Bsp1286I GDGCHC 1 cut(s) 614
Bsp13I TCCGGA 1 cut(s) 33
Bsp143I GATC 5 cut(s) 30, 635, 1094, 1182, 1294
Bsp19I CCATGG 3 cut(s) 83, 171, 918
BspACI CCGC 5 cut(s) 159, 232, 833, 1146, 1211
BspANI GGCC 4 cut(s) 92, 225, 828, 1014
BspCNI CTCAG 4 cut(s) 357, 361, 685, 1031
BspEI TCCGGA 1 cut(s) 33
BspLI GGNNCC 1 cut(s) 1290
BspPI GGATC 3 cut(s) 25, 1102, 1190
BspTI CTTAAG 1 cut(s) 107
BspTNI GGTCTC 1 cut(s) 200
BsrBI CCGCTC 1 cut(s) 1146
BsrDI GCAATG 2 cut(s) 216, 843
BsrFI RCCGGY 1 cut(s) 877
BsrI ACTGG 2 cut(s) 62, 1314
BssAI RCCGGY 1 cut(s) 877
BssECI CCNNGG 9 cut(s) 83, 171, 250, 444, 496, 508, 571, 887, 918
BssMI GATC 5 cut(s) 30, 635, 1094, 1182, 1294
BssNI GRCGYC 2 cut(s) 14, 207
BssT1I CCWWGG 6 cut(s) 83, 171, 250, 496, 508, 918
Bst2UI CCWGG 1 cut(s) 830
Bst4CI ACNGT 1 cut(s) 194
BstACI GRCGYC 2 cut(s) 14, 207
BstAFI CTTAAG 1 cut(s) 107
BstAPI GCANNNNNTGC 1 cut(s) 731
BstC8I GCNNGC 6 cut(s) 223, 227, 414, 452, 1167, 1215
BstDEI CTNAG 4 cut(s) 344, 369, 693, 1018
BstDSI CCRYGG 3 cut(s) 83, 171, 918
BstEII GGTNACC 1 cut(s) 913
BstENI CCTNNNNNAGG 1 cut(s) 1019
BstF5I GGATG 3 cut(s) 440, 477, 1091
BstH2I RGCGCY 1 cut(s) 324
BstHHI GCGC 2 cut(s) 323, 1165
BstKTI GATC 5 cut(s) 33, 638, 1097, 1185, 1297
BstMAI GTCTC 1 cut(s) 200
BstMBI GATC 5 cut(s) 30, 635, 1094, 1182, 1294
BstNI CCWGG 1 cut(s) 830
BstPI GGTNACC 1 cut(s) 913
BstSCI CCNGG 2 cut(s) 828, 1081
BstSFI CTRYAG 1 cut(s) 285
BstV1I GCAGC 1 cut(s) 734
BstV2I GAAGAC 1 cut(s) 468
BstX2I RGATCY 3 cut(s) 30, 635, 1094
BstXI CCANNNNNNTGG 1 cut(s) 1257
BstYI RGATCY 3 cut(s) 30, 635, 1094
BsuI GTATCC 2 cut(s) 47, 1280
BsuRI GGCC 4 cut(s) 92, 225, 828, 1014
BtgI CCRYGG 3 cut(s) 83, 171, 918
BtsCI GGATG 3 cut(s) 440, 477, 1091
BtsI GCAGTG 2 cut(s) 276, 489
BtsIMutI CAGTG 2 cut(s) 276, 489
Cac8I GCNNGC 6 cut(s) 223, 227, 414, 452, 1167, 1215
CfoI GCGC 2 cut(s) 323, 1165
Cfr10I RCCGGY 1 cut(s) 877
CseI GACGC 1 cut(s) 215
CspAI ACCGGT 1 cut(s) 877
CviAII CATG 7 cut(s) 84, 172, 276, 714, 919, 1266, 1298
DdeI CTNAG 4 cut(s) 344, 369, 693, 1018
DpnI GATC 5 cut(s) 32, 637, 1096, 1184, 1296
DpnII GATC 5 cut(s) 30, 635, 1094, 1182, 1294
EaeI YGGCCR 3 cut(s) 90, 223, 826
EciI GGCGGA 1 cut(s) 148
Ecl136II GAGCTC 1 cut(s) 612
Eco130I CCWWGG 6 cut(s) 83, 171, 250, 496, 508, 918
Eco24I GRGCYC 1 cut(s) 614
Eco31I GGTCTC 1 cut(s) 200
Eco32I GATATC 1 cut(s) 1151
Eco47III AGCGCT 1 cut(s) 322
Eco53kI GAGCTC 1 cut(s) 612
Eco57I CTGAAG 4 cut(s) 55, 440, 462, 1036
Eco88I CYCGRG 1 cut(s) 445
Eco91I GGTNACC 1 cut(s) 913
EcoICRI GAGCTC 1 cut(s) 612
EcoNI CCTNNNNNAGG 1 cut(s) 1019
EcoO65I GGTNACC 1 cut(s) 913
EcoRI GAATTC 1 cut(s) 648
EcoRII CCWGG 1 cut(s) 828
EcoRV GATATC 1 cut(s) 1151
EcoT14I CCWWGG 6 cut(s) 83, 171, 250, 496, 508, 918
EcoT38I GRGCYC 1 cut(s) 614
ErhI CCWWGG 6 cut(s) 83, 171, 250, 496, 508, 918
FaeI CATG 7 cut(s) 87, 175, 279, 717, 922, 1269, 1301
FatI CATG 7 cut(s) 83, 171, 275, 713, 918, 1265, 1297
FauNDI CATATG 1 cut(s) 730
Fnu4HI GCNGC 2 cut(s) 723, 834
FokI GGATG 3 cut(s) 427, 484, 1098
FriOI GRGCYC 1 cut(s) 614
Fsp4HI GCNGC 2 cut(s) 723, 834
FspBI CTAG 3 cut(s) 497, 509, 1154
GlaI GCGC 2 cut(s) 322, 1164
GluI GCNGC 2 cut(s) 723, 834
GsuI CTGGAG 2 cut(s) 125, 134
HaeII RGCGCY 1 cut(s) 324
HaeIII GGCC 4 cut(s) 92, 225, 828, 1014
HapII CCGG 4 cut(s) 34, 878, 967, 1082
HgaI GACGC 1 cut(s) 215
HhaI GCGC 2 cut(s) 323, 1165
Hin1I GRCGYC 2 cut(s) 14, 207
Hin1II CATG 7 cut(s) 87, 175, 279, 717, 922, 1269, 1301
Hin6I GCGC 2 cut(s) 321, 1163
HinP1I GCGC 2 cut(s) 321, 1163
HincII GTYRAC 1 cut(s) 97
HindII GTYRAC 1 cut(s) 97
HindIII AAGCTT 1 cut(s) 659
HinfI GANTC 7 cut(s) 117, 128, 492, 794, 810, 1042, 1283
HpaII CCGG 4 cut(s) 34, 878, 967, 1082
HphI GGTGA 2 cut(s) 22, 860
Hpy166II GTNNAC 3 cut(s) 97, 127, 431
Hpy188I TCNGA 9 cut(s) 22, 179, 205, 640, 667, 940, 985, 1021, 1288
Hpy188III TCNNGA 3 cut(s) 34, 151, 653
Hpy8I GTNNAC 3 cut(s) 97, 127, 431
Hpy99I CGWCG 3 cut(s) 19, 22, 209
HpyAV CCTTC 5 cut(s) 31, 632, 1060, 1228, 1315
HpyCH4III ACNGT 1 cut(s) 194
HpyCH4IV ACGT 1 cut(s) 14
HpyCH4V TGCA 7 cut(s) 64, 395, 680, 722, 953, 1121, 1169
HpyF3I CTNAG 4 cut(s) 344, 369, 693, 1018
HpySE526I ACGT 1 cut(s) 14
Hsp92I GRCGYC 2 cut(s) 14, 207
Hsp92II CATG 7 cut(s) 87, 175, 279, 717, 922, 1269, 1301
HspAI GCGC 2 cut(s) 321, 1163
Kpn2I TCCGGA 1 cut(s) 33
Kzo9I GATC 5 cut(s) 30, 635, 1094, 1182, 1294
LmnI GCTCC 2 cut(s) 150, 541
Lsp1109I GCAGC 1 cut(s) 734
LweI GCATC 6 cut(s) 318, 373, 734, 931, 1076, 1178
MaeI CTAG 3 cut(s) 497, 509, 1154
MaeII ACGT 1 cut(s) 14
MaeIII GTNAC 4 cut(s) 10, 866, 880, 913
MalI GATC 5 cut(s) 32, 637, 1096, 1184, 1296
MbiI CCGCTC 1 cut(s) 1146
MboI GATC 5 cut(s) 30, 635, 1094, 1182, 1294
MboII GAAGA 4 cut(s) 40, 94, 468, 645
MfeI CAATTG 2 cut(s) 363, 675
MflI RGATCY 3 cut(s) 30, 635, 1094
MhlI GDGCHC 1 cut(s) 614
MlsI TGGCCA 2 cut(s) 225, 828
MluCI AATT 8 cut(s) 315, 349, 363, 648, 675, 708, 897, 988
MluNI TGGCCA 2 cut(s) 225, 828
MlyI GAGTC 3 cut(s) 111, 122, 788
MmeI TCCRAC 3 cut(s) 157, 228, 625
Mox20I TGGCCA 2 cut(s) 225, 828
MroI TCCGGA 1 cut(s) 33
MscI TGGCCA 2 cut(s) 225, 828
MseI TTAA 3 cut(s) 108, 683, 1038
MslI CAYNNNNRTG 1 cut(s) 1084
Msp20I TGGCCA 2 cut(s) 225, 828
MspCI CTTAAG 1 cut(s) 107
MspI CCGG 4 cut(s) 34, 878, 967, 1082
MspR9I CCNGG 2 cut(s) 830, 1083
MunI CAATTG 2 cut(s) 363, 675
Mva1269I GAATGC 3 cut(s) 234, 594, 1056
MvaI CCWGG 1 cut(s) 830
NciI CCSGG 1 cut(s) 1083
NcoI CCATGG 3 cut(s) 83, 171, 918
NdeI CATATG 1 cut(s) 730
NdeII GATC 5 cut(s) 30, 635, 1094, 1182, 1294
NlaIII CATG 7 cut(s) 87, 175, 279, 717, 922, 1269, 1301
NlaIV GGNNCC 1 cut(s) 1290
NmuCI GTSAC 2 cut(s) 10, 866
PcsI WCGNNNNNNNCGW 1 cut(s) 23
PctI GAATGC 3 cut(s) 234, 594, 1056
PfeI GAWTC 4 cut(s) 492, 810, 1042, 1283
PflFI GACNNNGTC 1 cut(s) 16
PinAI ACCGGT 1 cut(s) 877
PkrI GCNGC 2 cut(s) 724, 835
PleI GAGTC 3 cut(s) 111, 122, 788
PpsI GAGTC 3 cut(s) 111, 122, 788
Psp124BI GAGCTC 1 cut(s) 614
Psp6I CCWGG 1 cut(s) 828
PspEI GGTNACC 1 cut(s) 913
PspGI CCWGG 1 cut(s) 828
PspN4I GGNNCC 1 cut(s) 1290
PsuI RGATCY 3 cut(s) 30, 635, 1094
PsyI GACNNNGTC 1 cut(s) 16
RseI CAYNNNNRTG 1 cut(s) 1084
SacI GAGCTC 1 cut(s) 614
SaqAI TTAA 3 cut(s) 108, 683, 1038
SatI GCNGC 2 cut(s) 723, 834
Sau3AI GATC 5 cut(s) 30, 635, 1094, 1182, 1294
SchI GAGTC 3 cut(s) 111, 122, 788
ScrFI CCNGG 2 cut(s) 830, 1083
SduI GDGCHC 1 cut(s) 614
SfaNI GCATC 6 cut(s) 318, 373, 734, 931, 1076, 1178
SfcI CTRYAG 1 cut(s) 285
SmiMI CAYNNNNRTG 1 cut(s) 1084
SmlI CTYRAG 1 cut(s) 107
SmoI CTYRAG 1 cut(s) 107
Sse9I AATT 8 cut(s) 315, 349, 363, 648, 675, 708, 897, 988
SsiI CCGC 5 cut(s) 159, 232, 833, 1146, 1211
SspI AATATT 3 cut(s) 782, 790, 1005
SspMI CTAG 3 cut(s) 497, 509, 1154
SstI GAGCTC 1 cut(s) 614
StyD4I CCNGG 2 cut(s) 828, 1081
StyI CCWWGG 6 cut(s) 83, 171, 250, 496, 508, 918
TaaI ACNGT 1 cut(s) 194
TaiI ACGT 1 cut(s) 17
TaqI TCGA 2 cut(s) 525, 652
TasI AATT 8 cut(s) 315, 349, 363, 648, 675, 708, 897, 988
TauI GCSGC 1 cut(s) 836
TfiI GAWTC 4 cut(s) 492, 810, 1042, 1283
Tru1I TTAA 3 cut(s) 108, 683, 1038
Tru9I TTAA 3 cut(s) 108, 683, 1038
TscAI CASTG 2 cut(s) 276, 489
TseFI GTSAC 2 cut(s) 10, 866
TseI GCWGC 1 cut(s) 722
Tsp45I GTSAC 2 cut(s) 10, 866
TspDTI ATGAA 2 cut(s) 577, 632
TspGWI ACGGA 1 cut(s) 129
TspRI CASTG 2 cut(s) 276, 489
Tth111I GACNNNGTC 1 cut(s) 16
Vha464I CTTAAG 1 cut(s) 107
XagI CCTNNNNNAGG 1 cut(s) 1019
XapI RAATTY 1 cut(s) 648
XcmI CCANNNNNNNNNTGG 1 cut(s) 169
XmaJI CCTAGG 2 cut(s) 496, 508
XspI CTAG 3 cut(s) 497, 509, 1154
ZraI GACGTC 1 cut(s) 15
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.