Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr08

Orthogroup ID

OG0006609

Physical Location & Seq

Genomic Coordinates Forward (+)

473952 .. 480099

Gene Structure 6148 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD08G1005300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1632 bp

ATGGCAAACGAGACCCAAGATGCTTCGCAGGCCACCGTTAATGGCGACAATGCGCAATCTCTCTGCGACGTCGTCCGAGGGTTGCCTAGTCGCGCCGTCGTAGACTTATGGTCCTCCGGCGTATGCACCAGATGCATCTTACGGTTGTTTGGGATCAGAGAGCATGATTACTATCGTTCTTCACTATCGCGGTCAATCTTGAGTTCAGTTCTTGGAGATTCAGAGAAACATTTGGTGGATAGTAATGTTAGAGAACCGGTAGTTTGTAGAGTTTGTTTGGGGATCTTGCAGTTCACTTATTCTGCGGATAACAAGTTGGTGGTAGAAAGGGAGAGTGCCAGTGAATTGGCTGTTTCCATTTCCGATCTGATTAGGCAACAGGGCTTTCAGATTGACGGCTTTTCTCTTGAAGTATCCATACCTCCAATTATCCAGGAAAATGATCGTATTGCTTGGCTATATATGAAAAGAAAGTTTGAATCTGAACTTTGGTTCCAAGGAAAATCACTCTCTGAATGCATTTCTGCAAAGGATGCTCTAAAAATTTCTCTTACGGAGATCCTTGAAACAATGTTGGGTTGTAAGTCCAGCATAAGCAGTTTCCGCATCCGTTTGACATACACTCACCGTATAGCAACAATAACAGTTGATAGTTCTAATGAGGGGTGCAAAAGAAGAAAAACAGGTTCAGGCAATGGTTTGGACAGAATTAATGATGACATCAAATGCTCTGGGTTTTCGACAAGTGAAGCTGATGCGGTTGTTCAAAGGTCTTTTGTGAGTTTGCAAGCTATTGAATCGCCTGAGTGCCTGAAGTTACCTCTAGAGCAGTCCAGTGAACCTTGCTGTTTAGAGTTTCTCTGCTACCGGACGCAAATTTATGTTGGTGGGAGATACCTGAAGTACTCGAGAAATGTGAGTCAATCCTGTTGGATTATTGATGATGAGAGAATGGGAGAAGCATCTGTCGAGGAGATACTAGGTAGCAACGTTCTTCCTATATGTCGTGGTGATAACTACAAGTTTCATGCTGCCGGAAGAGAAGATATCGATGTAAGGATGTTGGGTTCTGGAAGGCCGTTCCTGCTTGAGATACAAAACGCTCGCCTTGTCCCGTCTGATGACTCTGTAAAAAGTTTGGAAATAGCGATAAATAACTTGGAAAATAAATTGGTAGGCGTGAAAAATCTGAAGCTGGTTGATAGTCAGTCATGGGATCTAATGCGTGAAGGGGAAGCAGAGAAACAGAAGCAGTATGTTGCGTTAGTGTGGATTTCTCGCCCTCTCAAGGATGAGGACTTGCATTTTATATCTTCACTTAAAGACACTCATATTCAGCAGAGTACTCCCATCAGGGTGCTTCACCGCCGAAGTCCATTAGAACGAGAAAAGATCATACATTGGATGAGAATTGAGAAGATTGTTGGAAGTTCTCAGTATTTTCTTTTGCATCTGTGTACCCAGGCTGGAACGTACATTAAGGAATTCGTTCATGGGGATCTTGGACGCACCCAACCTAGCATTGGATCAATTTTTAGTTGTAGAGCAGAGATACTGCAGCTCGATGTAACAGATGACCGTAATTCTAGAAAACCACCCTGCTGCAGCTGCGGCTTTTTGGGGTTGATCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

No domains found.

Protein Analysis

544

Amino Acids

61.09

Weight (kDa)

6.14

Isoelectric Point (pI)

53.1

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pus10_N_euk	PF21237	89 - 209	9.6e-09	Eukaryotic Pus10, N-terminal
Pus10_C	PF21238	294 - 525	3.1e-84	Pus10, C-terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006609)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G20410 AT1G20410
fragaria_vesca	FvH4_2g37810 FvH4_2g37810 FvH4_2g37810 FvH4_2g37810 FvH4_2g37810 FvH4_2g37810 FvH4_2g37810
malus_domestica	MD08G1005300.v1.1 MD15G1004600.v1.1 MD15G1004800.v1.1
prunus_persica	Prupe.1G357400_v2.0.a1 Prupe.1G357400_v2.0.a1 Prupe.1G357400_v2.0.a1
rosa_chinensis	RchiOBHm_Chr6g0303901
rosa_laevigata	RLG00000011036
rosa_multiflora	Rmu_co8406005.1_g000001
rosa_roxburghii	Rroxscaffold_7G00164290
rosa_rugosa	Rorug06G0325700 Rorug06G0325800
rosa_samantha	Rh6AG440000 Rh6BG463000 Rh6CG452300 Rh6DG439300
rosa_wichuraiana	Rw6G038100

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	72
Acc16I	TGCGCA	1 cut(s)	54
AccI	GTMKAC	1 cut(s)	102
AccII	CGCG	2 cut(s)	93, 190
AciI	CCGC	6 cut(s)	190, 305, 604, 758, 1366, 1611
AclI	AACGTT	1 cut(s)	990
AclWI	GGATC	6 cut(s)	161, 290, 553, 1224, 1506, 1534
AcsI	RAATTY	3 cut(s)	543, 876, 1484
AcuI	CTGAAG	3 cut(s)	833, 920, 1211
AcyI	GRCGYC	1 cut(s)	69
AfaI	GTAC	4 cut(s)	905, 1345, 1459, 1475
AfiI	CCNNNNNNNGG	2 cut(s)	1288, 1523
AgeI	ACCGGT	1 cut(s)	256
AgsI	TTSAA	5 cut(s)	410, 479, 566, 767, 797
AhdI	GACNNNNNGTC	1 cut(s)	109
AjnI	CCWGG	2 cut(s)	432, 1461
AloI	GAACNNNNNNTCC	4 cut(s)	477, 509, 1051, 1083
AluBI	AGCT	5 cut(s)	752, 791, 1195, 1561, 1608
AluI	AGCT	5 cut(s)	752, 791, 1195, 1561, 1608
Alw26I	GTCTC	1 cut(s)	5
AlwI	GGATC	6 cut(s)	161, 290, 553, 1224, 1506, 1534
Ama87I	CYCGRG	1 cut(s)	907
AoxI	GGCC	2 cut(s)	30, 1076
ApeKI	GCWGC	5 cut(s)	1031, 1558, 1602, 1605, 1608
ApoI	RAATTY	3 cut(s)	543, 876, 1484
AseI	ATTAAT	1 cut(s)	711
AsiGI	ACCGGT	1 cut(s)	256
Asp700I	GAANNNNTTC	1 cut(s)	1488
AspLEI	GCGC	2 cut(s)	55, 95
AspS9I	GGNCC	1 cut(s)	111
AsuHPI	GGTGA	3 cut(s)	617, 1022, 1355
AvaI	CYCGRG	1 cut(s)	907
AvaII	GGWCC	1 cut(s)	111
BarI	GAAGNNNNNNTAC	2 cut(s)	402, 434
BbvI	GCAGC	5 cut(s)	1018, 1570, 1589, 1595, 1617
BccI	CCATC	1 cut(s)	1358
BceAI	ACGGC	3 cut(s)	80, 412, 1063
BciT130I	CCWGG	2 cut(s)	434, 1463
BciVI	GTATCC	1 cut(s)	424
BcoDI	GTCTC	1 cut(s)	5
BfaI	CTAG	5 cut(s)	87, 824, 980, 1518, 1587
BfmI	CTRYAG	2 cut(s)	1556, 1603
BfuI	GTATCC	1 cut(s)	424
BisI	GCNGC	6 cut(s)	1032, 1559, 1603, 1606, 1609, 1612
BlsI	GCNGC	6 cut(s)	1033, 1560, 1604, 1607, 1610, 1613
BmcAI	AGTACT	2 cut(s)	905, 1345
Bme1390I	CCNGG	2 cut(s)	434, 1463
Bme18I	GGWCC	1 cut(s)	111
BmeRI	GACNNNNNGTC	1 cut(s)	109
BmeT110I	CYCGRG	1 cut(s)	907
BmgT120I	GGNCC	1 cut(s)	111
BmiI	GGNNCC	1 cut(s)	494
BmrFI	CCNGG	2 cut(s)	434, 1463
BmsI	GCATC	8 cut(s)	10, 122, 144, 523, 615, 745, 971, 1459
BpuEI	CTTGAG	3 cut(s)	220, 1109, 1271
Bsa29I	ATCGAT	1 cut(s)	1050
BsaBI	GATNNNNATC	1 cut(s)	171
BsaHI	GRCGYC	1 cut(s)	69
BsaI	GGTCTC	1 cut(s)	5
BsaJI	CCNNGG	3 cut(s)	76, 496, 1461
BsaWI	WCCGGW	2 cut(s)	256, 867
Bsc4I	CCNNNNNNNGG	2 cut(s)	1288, 1523
Bse118I	RCCGGY	1 cut(s)	256
Bse1I	ACTGG	2 cut(s)	339, 834
Bse3DI	GCAATG	1 cut(s)	700
Bse8I	GATNNNNATC	1 cut(s)	171
BseBI	CCWGG	2 cut(s)	434, 1463
BseCI	ATCGAT	1 cut(s)	1050
BseDI	CCNNGG	3 cut(s)	76, 496, 1461
BseGI	GGATG	5 cut(s)	538, 606, 1065, 1297, 1410
BseJI	GATNNNNATC	1 cut(s)	171
BseLI	CCNNNNNNNGG	2 cut(s)	1288, 1523
BseMI	GCAATG	1 cut(s)	700
BseMII	CTCAG	2 cut(s)	795, 1448
BseNI	ACTGG	2 cut(s)	339, 834
BseRI	GAGGAG	1 cut(s)	986
BseXI	GCAGC	5 cut(s)	1018, 1570, 1589, 1595, 1617
Bsh1236I	CGCG	2 cut(s)	93, 190
BshFI	GGCC	2 cut(s)	32, 1078
BshTI	ACCGGT	1 cut(s)	256
BshVI	ATCGAT	1 cut(s)	1050
BsiHKCI	CYCGRG	1 cut(s)	907
BsiSI	CCGG	4 cut(s)	117, 257, 868, 1035
BslFI	GGGAC	1 cut(s)	1097
BslI	CCNNNNNNNGG	2 cut(s)	1288, 1523
BsmAI	GTCTC	1 cut(s)	5
BsmFI	GGGAC	1 cut(s)	1097
BsmI	GAATGC	1 cut(s)	521
BsnI	GGCC	2 cut(s)	32, 1078
Bso31I	GGTCTC	1 cut(s)	5
BsoBI	CYCGRG	1 cut(s)	907
BspACI	CCGC	6 cut(s)	190, 305, 604, 758, 1366, 1611
BspANI	GGCC	2 cut(s)	32, 1078
BspCNI	CTCAG	2 cut(s)	796, 1447
BspDI	ATCGAT	1 cut(s)	1050
BspFNI	CGCG	2 cut(s)	93, 190
BspLI	GGNNCC	1 cut(s)	494
BspMAI	CTGCAG	2 cut(s)	1560, 1607
BspPI	GGATC	6 cut(s)	161, 290, 553, 1224, 1506, 1534
BspTNI	GGTCTC	1 cut(s)	5
BsrDI	GCAATG	1 cut(s)	700
BsrFI	RCCGGY	1 cut(s)	256
BsrI	ACTGG	2 cut(s)	339, 834
BssAI	RCCGGY	1 cut(s)	256
BssECI	CCNNGG	3 cut(s)	76, 496, 1461
BssNI	GRCGYC	1 cut(s)	69
BssT1I	CCWWGG	1 cut(s)	496
Bst2UI	CCWGG	2 cut(s)	434, 1463
Bst4CI	ACNGT	5 cut(s)	37, 144, 629, 646, 1580
Bst6I	CTCTTC	1 cut(s)	1033
BstACI	GRCGYC	1 cut(s)	69
BstAPI	GCANNNNNTGC	2 cut(s)	132, 533
BstC8I	GCNNGC	3 cut(s)	30, 789, 1105
BstDEI	CTNAG	2 cut(s)	804, 1434
BstF5I	GGATG	5 cut(s)	538, 606, 1065, 1297, 1410
BstFNI	CGCG	2 cut(s)	93, 190
BstHHI	GCGC	2 cut(s)	55, 95
BstMAI	GTCTC	1 cut(s)	5
BstMWI	GCNNNNNNNGC	7 cut(s)	29, 132, 533, 603, 1084, 1608, 1611
BstNI	CCWGG	2 cut(s)	434, 1463
BstSCI	CCNGG	2 cut(s)	432, 1461
BstSFI	CTRYAG	2 cut(s)	1556, 1603
BstUI	CGCG	2 cut(s)	93, 190
BstV1I	GCAGC	5 cut(s)	1018, 1570, 1589, 1595, 1617
BstX2I	RGATCY	4 cut(s)	282, 558, 1216, 1498
BstXI	CCANNNNNNTGG	1 cut(s)	346
BstYI	RGATCY	4 cut(s)	282, 558, 1216, 1498
Bsu15I	ATCGAT	1 cut(s)	1050
BsuI	GTATCC	1 cut(s)	424
BsuRI	GGCC	2 cut(s)	32, 1078
BsuTUI	ATCGAT	1 cut(s)	1050
BtsCI	GGATG	5 cut(s)	538, 606, 1065, 1297, 1410
BtsIMutI	CAGTG	2 cut(s)	346, 841
Cac8I	GCNNGC	3 cut(s)	30, 789, 1105
CfoI	GCGC	2 cut(s)	55, 95
Cfr10I	RCCGGY	1 cut(s)	256
Cfr13I	GGNCC	1 cut(s)	111
ClaI	ATCGAT	1 cut(s)	1050
CseI	GACGC	2 cut(s)	880, 1515
Csp6I	GTAC	4 cut(s)	904, 1344, 1458, 1474
CspAI	ACCGGT	1 cut(s)	256
CviAII	CATG	4 cut(s)	164, 1028, 1212, 1493
CviQI	GTAC	4 cut(s)	904, 1344, 1458, 1474
DdeI	CTNAG	2 cut(s)	804, 1434
DriI	GACNNNNNGTC	1 cut(s)	109
Eam1104I	CTCTTC	1 cut(s)	1033
Eam1105I	GACNNNNNGTC	1 cut(s)	109
EarI	CTCTTC	1 cut(s)	1033
Eco130I	CCWWGG	1 cut(s)	496
Eco31I	GGTCTC	1 cut(s)	5
Eco32I	GATATC	1 cut(s)	1048
Eco47I	GGWCC	1 cut(s)	111
Eco57I	CTGAAG	3 cut(s)	833, 920, 1211
Eco88I	CYCGRG	1 cut(s)	907
EcoRI	GAATTC	1 cut(s)	1484
EcoRII	CCWGG	2 cut(s)	432, 1461
EcoRV	GATATC	1 cut(s)	1048
EcoT14I	CCWWGG	1 cut(s)	496
EcoT22I	ATGCAT	2 cut(s)	137, 521
ErhI	CCWWGG	1 cut(s)	496
FaeI	CATG	4 cut(s)	167, 1031, 1215, 1496
FaqI	GGGAC	1 cut(s)	1097
FatI	CATG	4 cut(s)	163, 1027, 1211, 1492
FblI	GTMKAC	1 cut(s)	102
Fnu4HI	GCNGC	6 cut(s)	1032, 1559, 1603, 1606, 1609, 1612
FokI	GGATG	5 cut(s)	545, 593, 1072, 1304, 1417
Fsp4HI	GCNGC	6 cut(s)	1032, 1559, 1603, 1606, 1609, 1612
FspBI	CTAG	5 cut(s)	87, 824, 980, 1518, 1587
FspI	TGCGCA	1 cut(s)	54
GlaI	GCGC	2 cut(s)	54, 94
GluI	GCNGC	6 cut(s)	1032, 1559, 1603, 1606, 1609, 1612
HaeIII	GGCC	2 cut(s)	32, 1078
HapII	CCGG	4 cut(s)	117, 257, 868, 1035
HgaI	GACGC	2 cut(s)	880, 1515
HhaI	GCGC	2 cut(s)	55, 95
Hin1I	GRCGYC	1 cut(s)	69
Hin1II	CATG	4 cut(s)	167, 1031, 1215, 1496
Hin6I	GCGC	2 cut(s)	53, 93
HinP1I	GCGC	2 cut(s)	53, 93
HinfI	GANTC	5 cut(s)	218, 479, 797, 919, 1124
HpaII	CCGG	4 cut(s)	117, 257, 868, 1035
HphI	GGTGA	3 cut(s)	617, 1022, 1355
Hpy166II	GTNNAC	4 cut(s)	103, 294, 839, 1458
Hpy188III	TCNNGA	6 cut(s)	199, 407, 824, 909, 1071, 1587
Hpy8I	GTNNAC	4 cut(s)	103, 294, 839, 1458
Hpy99I	CGWCG	3 cut(s)	71, 74, 101
HpyAV	CCTTC	2 cut(s)	1068, 1223
HpyCH4III	ACNGT	5 cut(s)	37, 144, 629, 646, 1580
HpyCH4IV	ACGT	3 cut(s)	69, 990, 1472
HpyF10VI	GCNNNNNNNGC	7 cut(s)	29, 132, 533, 603, 1084, 1608, 1611
HpyF3I	CTNAG	2 cut(s)	804, 1434
HpySE526I	ACGT	3 cut(s)	69, 990, 1472
Hsp92I	GRCGYC	1 cut(s)	69
Hsp92II	CATG	4 cut(s)	167, 1031, 1215, 1496
HspAI	GCGC	2 cut(s)	53, 93
Lsp1109I	GCAGC	5 cut(s)	1018, 1570, 1589, 1595, 1617
LweI	GCATC	8 cut(s)	10, 122, 144, 523, 615, 745, 971, 1459
MaeI	CTAG	5 cut(s)	87, 824, 980, 1518, 1587
MaeII	ACGT	3 cut(s)	69, 990, 1472
MaeIII	GTNAC	2 cut(s)	816, 1567
MboII	GAAGA	7 cut(s)	171, 687, 986, 1050, 1055, 1305, 1429
MflI	RGATCY	4 cut(s)	282, 558, 1216, 1498
MlyI	GAGTC	2 cut(s)	928, 1118
MmeI	TCCRAC	2 cut(s)	911, 1405
MnlI	CCTC	8 cut(s)	71, 124, 432, 655, 831, 964, 1288, 1293
Mph1103I	ATGCAT	2 cut(s)	137, 521
MroXI	GAANNNNTTC	1 cut(s)	1488
MseI	TTAA	4 cut(s)	39, 711, 1320, 1479
MslI	CAYNNNNRTG	1 cut(s)	1355
MspA1I	CMGCKG	1 cut(s)	1608
MspI	CCGG	4 cut(s)	117, 257, 868, 1035
MspR9I	CCNGG	2 cut(s)	434, 1463
Mva1269I	GAATGC	1 cut(s)	521
MvaI	CCWGG	2 cut(s)	434, 1463
MvnI	CGCG	2 cut(s)	93, 190
MwoI	GCNNNNNNNGC	7 cut(s)	29, 132, 533, 603, 1084, 1608, 1611
NlaIII	CATG	4 cut(s)	167, 1031, 1215, 1496
NlaIV	GGNNCC	1 cut(s)	494
NsbI	TGCGCA	1 cut(s)	54
NsiI	ATGCAT	2 cut(s)	137, 521
PaeR7I	CTCGAG	1 cut(s)	907
PctI	GAATGC	1 cut(s)	521
PdmI	GAANNNNTTC	1 cut(s)	1488
PfeI	GAWTC	3 cut(s)	218, 479, 797
PflFI	GACNNNGTC	1 cut(s)	71
PfoI	TCCNGGA	1 cut(s)	432
PinAI	ACCGGT	1 cut(s)	256
PkrI	GCNGC	6 cut(s)	1033, 1560, 1604, 1607, 1610, 1613
PleI	GAGTC	2 cut(s)	927, 1118
PpsI	GAGTC	2 cut(s)	927, 1118
PshBI	ATTAAT	1 cut(s)	711
Psp1406I	AACGTT	1 cut(s)	990
Psp6I	CCWGG	2 cut(s)	432, 1461
PspGI	CCWGG	2 cut(s)	432, 1461
PspN4I	GGNNCC	1 cut(s)	494
PspPI	GGNCC	1 cut(s)	111
PstI	CTGCAG	2 cut(s)	1560, 1607
PsuI	RGATCY	4 cut(s)	282, 558, 1216, 1498
PsyI	GACNNNGTC	1 cut(s)	71
PvuII	CAGCTG	1 cut(s)	1608
RsaI	GTAC	4 cut(s)	905, 1345, 1459, 1475
RsaNI	GTAC	4 cut(s)	904, 1344, 1458, 1474
RseI	CAYNNNNRTG	1 cut(s)	1355
SaqAI	TTAA	4 cut(s)	39, 711, 1320, 1479
SatI	GCNGC	6 cut(s)	1032, 1559, 1603, 1606, 1609, 1612
Sau96I	GGNCC	1 cut(s)	111
ScaI	AGTACT	2 cut(s)	905, 1345
SchI	GAGTC	2 cut(s)	928, 1118
ScrFI	CCNGG	2 cut(s)	434, 1463
SfaNI	GCATC	8 cut(s)	10, 122, 144, 523, 615, 745, 971, 1459
SfcI	CTRYAG	2 cut(s)	1556, 1603
Sfr274I	CTCGAG	1 cut(s)	907
SinI	GGWCC	1 cut(s)	111
SlaI	CTCGAG	1 cut(s)	907
SmiMI	CAYNNNNRTG	1 cut(s)	1355
SmlI	CTYRAG	4 cut(s)	199, 907, 1088, 1286
SmoI	CTYRAG	4 cut(s)	199, 907, 1088, 1286
SsiI	CCGC	6 cut(s)	190, 305, 604, 758, 1366, 1611
SspMI	CTAG	5 cut(s)	87, 824, 980, 1518, 1587
StyD4I	CCNGG	2 cut(s)	432, 1461
StyI	CCWWGG	1 cut(s)	496
TaaI	ACNGT	5 cut(s)	37, 144, 629, 646, 1580
TaiI	ACGT	3 cut(s)	72, 993, 1475
TaqI	TCGA	5 cut(s)	740, 908, 969, 1050, 1563
TatI	WGTACW	2 cut(s)	903, 1343
TauI	GCSGC	1 cut(s)	1614
TfiI	GAWTC	3 cut(s)	218, 479, 797
Tru1I	TTAA	4 cut(s)	39, 711, 1320, 1479
Tru9I	TTAA	4 cut(s)	39, 711, 1320, 1479
TscAI	CASTG	2 cut(s)	346, 841
TseI	GCWGC	5 cut(s)	1031, 1558, 1602, 1605, 1608
TspDTI	ATGAA	3 cut(s)	479, 1016, 1481
TspGWI	ACGGA	2 cut(s)	569, 599
TspRI	CASTG	2 cut(s)	346, 841
Tth111I	GACNNNGTC	1 cut(s)	71
VpaK11BI	GGWCC	1 cut(s)	111
VspI	ATTAAT	1 cut(s)	711
XapI	RAATTY	3 cut(s)	543, 876, 1484
XbaI	TCTAGA	2 cut(s)	823, 1586
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1520
XhoI	CTCGAG	1 cut(s)	907
XmiI	GTMKAC	1 cut(s)	102
XmnI	GAANNNNTTC	1 cut(s)	1488
XspI	CTAG	5 cut(s)	87, 824, 980, 1518, 1587
ZraI	GACGTC	1 cut(s)	70
ZrmI	AGTACT	2 cut(s)	905, 1345
Zsp2I	ATGCAT	2 cut(s)	137, 521

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.