Rmu_co8406005.1_g000001

tRNA pseudouridine synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_co8406005.1
OG0006609
Physical Location & Seq
Reverse (-)
1 .. 1264
1264 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_co8406005.1_g000001.1.cds

Sequence Viewer

Length: 351 bp
atgggtagcaacattcttcctatatgtcgtggtgacaagtacaaatttcatgctgcgggcagagaagatattgatgttcgaatgttgggttccggaaggcctttcctggttgaggtacaaaatgcacgctatgtcccatcgcaggagtctgtaaaagatctgcaaacaaagataaataatttgcaaaataaattggttggtgtgaaaaatctgaaactggtgggcagtcaatcatgggatctcatgcgtgaaggagaagcagagaaacagaagcaatacactgcacttgtgtggacttctcgcattctcaaggatgaagacttgcagtctatatcttcaattaaggacatg
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

117

Amino Acids

13.3

Weight (kDa)

9.07

Isoelectric Point (pI)

47.96

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 92
AciI CCGC 1 cut(s) 56
AclWI GGATC 1 cut(s) 246
AcsI RAATTY 1 cut(s) 44
AfaI GTAC 2 cut(s) 41, 117
AfiI CCNNNNNNNGG 2 cut(s) 112, 142
AgsI TTSAA 1 cut(s) 339
AhdI GACNNNNNGTC 1 cut(s) 325
AjnI CCWGG 1 cut(s) 105
AleI CACNNNNGTG 1 cut(s) 289
AlwI GGATC 1 cut(s) 246
Aor13HI TCCGGA 1 cut(s) 92
AoxI GGCC 1 cut(s) 98
ApeKI GCWGC 1 cut(s) 53
ApoI RAATTY 1 cut(s) 44
AsuHPI GGTGA 1 cut(s) 44
AsuII TTCGAA 1 cut(s) 79
BbsI GAAGAC 1 cut(s) 324
BbvI GCAGC 1 cut(s) 40
BccI CCATC 1 cut(s) 145
BciT130I CCWGG 1 cut(s) 107
BglII AGATCT 1 cut(s) 157
BisI GCNGC 1 cut(s) 54
BlsI GCNGC 1 cut(s) 55
Bme1390I CCNGG 1 cut(s) 107
BmeRI GACNNNNNGTC 1 cut(s) 325
BmiI GGNNCC 1 cut(s) 91
BmrFI CCNGG 1 cut(s) 107
BpiI GAAGAC 1 cut(s) 324
Bpu14I TTCGAA 1 cut(s) 79
BpuEI CTTGAG 1 cut(s) 293
BsaWI WCCGGW 1 cut(s) 92
Bsc4I CCNNNNNNNGG 2 cut(s) 112, 142
Bse1I ACTGG 1 cut(s) 222
BseAI TCCGGA 1 cut(s) 92
BseBI CCWGG 1 cut(s) 107
BseGI GGATG 1 cut(s) 319
BseLI CCNNNNNNNGG 2 cut(s) 112, 142
BseNI ACTGG 1 cut(s) 222
BseXI GCAGC 1 cut(s) 40
BsgI GTGCAG 1 cut(s) 267
BshFI GGCC 1 cut(s) 100
BsiSI CCGG 1 cut(s) 93
BslFI GGGAC 1 cut(s) 119
BslI CCNNNNNNNGG 2 cut(s) 112, 142
BsmFI GGGAC 1 cut(s) 119
BsmI GAATGC 1 cut(s) 303
BsnI GGCC 1 cut(s) 100
Bsp119I TTCGAA 1 cut(s) 79
Bsp13I TCCGGA 1 cut(s) 92
Bsp143I GATC 2 cut(s) 157, 238
BspACI CCGC 1 cut(s) 56
BspANI GGCC 1 cut(s) 100
BspEI TCCGGA 1 cut(s) 92
BspLI GGNNCC 1 cut(s) 91
BspPI GGATC 1 cut(s) 246
BspT104I TTCGAA 1 cut(s) 79
BsrI ACTGG 1 cut(s) 222
BssMI GATC 2 cut(s) 157, 238
Bst2UI CCWGG 1 cut(s) 107
BstBI TTCGAA 1 cut(s) 79
BstC8I GCNNGC 2 cut(s) 58, 127
BstENI CCTNNNNNAGG 1 cut(s) 110
BstF5I GGATG 1 cut(s) 319
BstKTI GATC 2 cut(s) 160, 241
BstMBI GATC 2 cut(s) 157, 238
BstNI CCWGG 1 cut(s) 107
BstSCI CCNGG 1 cut(s) 105
BstV1I GCAGC 1 cut(s) 40
BstV2I GAAGAC 1 cut(s) 324
BstX2I RGATCY 2 cut(s) 157, 238
BstYI RGATCY 2 cut(s) 157, 238
BsuRI GGCC 1 cut(s) 100
BtgZI GCGATG 1 cut(s) 123
BtsCI GGATG 1 cut(s) 319
BtsI GCAGTG 1 cut(s) 279
BtsIMutI CAGTG 1 cut(s) 279
Cac8I GCNNGC 2 cut(s) 58, 127
Csp6I GTAC 2 cut(s) 40, 116
CviAII CATG 4 cut(s) 50, 234, 244, 349
CviJI RGCY 1 cut(s) 100
CviKI_1 RGCY 1 cut(s) 100
CviQI GTAC 2 cut(s) 40, 116
DpnI GATC 2 cut(s) 159, 240
DpnII GATC 2 cut(s) 157, 238
DriI GACNNNNNGTC 1 cut(s) 325
Eam1105I GACNNNNNGTC 1 cut(s) 325
Eco147I AGGCCT 1 cut(s) 100
EcoNI CCTNNNNNAGG 1 cut(s) 110
EcoRII CCWGG 1 cut(s) 105
FaeI CATG 3 cut(s) 53, 237, 247
FaiI YATR 8 cut(s) 23, 25, 51, 132, 235, 245, 332, 350
FaqI GGGAC 1 cut(s) 119
FatI CATG 3 cut(s) 49, 233, 243
FauI CCCGC 1 cut(s) 49
Fnu4HI GCNGC 1 cut(s) 54
FokI GGATG 1 cut(s) 326
Fsp4HI GCNGC 1 cut(s) 54
GluI GCNGC 1 cut(s) 54
HaeIII GGCC 1 cut(s) 100
HapII CCGG 1 cut(s) 93
Hin1II CATG 3 cut(s) 53, 237, 247
HinfI GANTC 1 cut(s) 146
HpaII CCGG 1 cut(s) 93
HphI GGTGA 1 cut(s) 44
Hpy166II GTNNAC 1 cut(s) 294
Hpy188I TCNGA 1 cut(s) 213
Hpy188III TCNNGA 1 cut(s) 93
Hpy8I GTNNAC 1 cut(s) 294
HpyAV CCTTC 2 cut(s) 90, 245
HpyCH4V TGCA 5 cut(s) 125, 163, 184, 284, 325
Hsp92II CATG 3 cut(s) 53, 237, 247
Kpn2I TCCGGA 1 cut(s) 92
Kzo9I GATC 2 cut(s) 157, 238
LpnPI CCDG 5 cut(s) 92, 106, 119, 128, 203
Lsp1109I GCAGC 1 cut(s) 40
MaeIII GTNAC 1 cut(s) 32
MalI GATC 2 cut(s) 159, 240
MboI GATC 2 cut(s) 157, 238
MboII GAAGA 4 cut(s) 8, 77, 327, 329
MflI RGATCY 2 cut(s) 157, 238
MluCI AATT 4 cut(s) 44, 178, 191, 339
MlyI GAGTC 1 cut(s) 155
MnlI CCTC 1 cut(s) 106
MroI TCCGGA 1 cut(s) 92
MseI TTAA 1 cut(s) 342
MslI CAYNNNNRTG 1 cut(s) 289
MspI CCGG 1 cut(s) 93
MspR9I CCNGG 1 cut(s) 107
Mva1269I GAATGC 1 cut(s) 303
MvaI CCWGG 1 cut(s) 107
NdeII GATC 2 cut(s) 157, 238
NlaIII CATG 3 cut(s) 53, 237, 247
NlaIV GGNNCC 1 cut(s) 91
NmuCI GTSAC 1 cut(s) 32
NspV TTCGAA 1 cut(s) 79
OliI CACNNNNGTG 1 cut(s) 289
PceI AGGCCT 1 cut(s) 100
PctI GAATGC 1 cut(s) 303
PkrI GCNGC 1 cut(s) 55
PleI GAGTC 1 cut(s) 154
PpsI GAGTC 1 cut(s) 154
Psp6I CCWGG 1 cut(s) 105
PspGI CCWGG 1 cut(s) 105
PspN4I GGNNCC 1 cut(s) 91
PsuI RGATCY 2 cut(s) 157, 238
RsaI GTAC 2 cut(s) 41, 117
RsaNI GTAC 2 cut(s) 40, 116
RseI CAYNNNNRTG 1 cut(s) 289
SaqAI TTAA 1 cut(s) 342
SatI GCNGC 1 cut(s) 54
Sau3AI GATC 2 cut(s) 157, 238
SchI GAGTC 1 cut(s) 155
ScrFI CCNGG 1 cut(s) 107
SetI ASST 1 cut(s) 117
SfuI TTCGAA 1 cut(s) 79
SmiMI CAYNNNNRTG 1 cut(s) 289
SmlI CTYRAG 1 cut(s) 308
SmoI CTYRAG 1 cut(s) 308
Sse9I AATT 4 cut(s) 44, 178, 191, 339
SseBI AGGCCT 1 cut(s) 100
SsiI CCGC 1 cut(s) 56
StuI AGGCCT 1 cut(s) 100
StyD4I CCNGG 1 cut(s) 105
TaqI TCGA 1 cut(s) 79
TasI AATT 4 cut(s) 44, 178, 191, 339
TatI WGTACW 1 cut(s) 39
Tru1I TTAA 1 cut(s) 342
Tru9I TTAA 1 cut(s) 342
TscAI CASTG 1 cut(s) 286
TseFI GTSAC 1 cut(s) 32
TseI GCWGC 1 cut(s) 53
Tsp45I GTSAC 1 cut(s) 32
TspDTI ATGAA 2 cut(s) 38, 330
TspRI CASTG 1 cut(s) 286
XagI CCTNNNNNAGG 1 cut(s) 110
XapI RAATTY 1 cut(s) 44
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.