MD08G1034800.v1.1

Protein of unknown function (DUF502)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0015365
Physical Location & Seq
Reverse (-)
2446589 .. 2449091
2503 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1034800.v1.1.491

Sequence Viewer

Length: 936 bp
ATGGCGACCAGAGAGAGAGAAAGGGAAAGGGATCTCGAGCTCCTCATCCCAGTCGCTGCCGTTTCCGAAACCGGAGGAGATTCCAAATCGTCCTCCCCAGCAAACTCCCCACCTCCCACCACCTCCCATTCCCATCATCAAACCGGCCGCGAGGCCTTTTCGAAAGTTATTCAGAGCTGGGCTTCCAAGAAGTTTATGACAGGATGTGTCATTCTTCTTCCAATAGCTATTACATTCTACGTCACATGGGCTTTCATTAAATTTGTTGATGGTTTCTTCTCCCCAATCTACAATCACCTCGGGATCAATATTTTCGGTCTTGGATTTGCTACCTCCCTCACCTTCATCTTCTTAGTCGGCATTTTCATGCAATCGTGGTTGGGAACTTCTGTTCTTACTCTTTGGGAGTGGTTTATTAAGAAAATGCCGCTTATAAGCTATATCTACGCTGCATCAAAACAAATTAGCATAGCAATTTCACCAGATCAGAGCTCGAAAGCCTTTAAGGAAGTGGCGATCATAAGGCATCCAAGAATTGGGGAGTATGCATTAGGGTTTATCACTTCAACTGTTGTTCTTTCTCAAAGAAACGTAGGAGAAGAACTATGCTGTGTTTATATACCTACGAACCACCTTTATCTTGGGGATATTTTTCTGATCAGTCCCAAGGATATTCTGAGACCTAATCTGTCCGTTCGAGAGGGGATTGAAATTGTCATCTCCGGAGGTCTATCCATACCGCAACTTTTAACTACGGTGGATGCACAAGTCCTTACACCAAGAAGAGTTCCGCACTTTGAAGAGCCAAAAAAGTTGTCAAACTCAAACAAAGTTTTGCAGAGGAAGTTTAGAGGCTCAAAAGCGGCCGGATCGTACCGGGAATGGAGTGAAGAACACCATAGCAGAATACTGAAATGCAGTAAGAAGCCCCTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF502

Protein Analysis

312

Amino Acids

34.84

Weight (kDa)

9.46

Isoelectric Point (pI)

40.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF502 PF04367 112 - 211 7.3e-25 Protein of unknown function (DUF502)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015365)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G18460
fragaria_vesca FvH4_2g39890 FvH4_2g39890 FvH4_2g39890
malus_domestica MD08G1034800.v1.1 MD15G1031600.v1.1
prunus_persica Prupe.1G380400_v2.0.a1
pyrus_communis pycom15g02730
rosa_chinensis RchiOBHm_Chr6g0300911
rosa_laevigata RLG00000011291
rosa_multiflora Rmu_sc0006254.1_g000003
rosa_roxburghii Rroxscaffold_7G00167320
rosa_samantha Rh6AG413000 Rh6BG420200 Rh6CG427700 Rh6DG413000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 434
AccB7I CCANNNNNTGG 1 cut(s) 536
AccII CGCG 1 cut(s) 150
AccIII TCCGGA 1 cut(s) 722
AciI CCGC 5 cut(s) 148, 428, 740, 791, 863
AclWI GGATC 3 cut(s) 39, 311, 877
AcoI YGGCCR 2 cut(s) 145, 864
AcsI RAATTY 1 cut(s) 260
AfaI GTAC 1 cut(s) 875
AfiI CCNNNNNNNGG 1 cut(s) 536
AgsI TTSAA 3 cut(s) 567, 710, 800
AloI GAACNNNNNNTCC 2 cut(s) 375, 407
AluBI AGCT 5 cut(s) 40, 177, 227, 438, 492
AluI AGCT 5 cut(s) 40, 177, 227, 438, 492
Alw21I GWGCWC 2 cut(s) 42, 494
Alw26I GTCTC 1 cut(s) 673
AlwI GGATC 3 cut(s) 39, 311, 877
AlwNI CAGNNNCTG 1 cut(s) 56
Ama87I CYCGRG 2 cut(s) 35, 299
Aor13HI TCCGGA 1 cut(s) 722
AoxI GGCC 3 cut(s) 145, 153, 864
ApeKI GCWGC 2 cut(s) 56, 449
ApoI RAATTY 1 cut(s) 260
ArsI GACNNNNNNTTYG 2 cut(s) 800, 832
AsuC2I CCSGG 1 cut(s) 878
AsuHPI GGTGA 3 cut(s) 287, 331, 471
AsuII TTCGAA 1 cut(s) 161
AvaI CYCGRG 2 cut(s) 35, 299
BanII GRGCYC 2 cut(s) 42, 494
Bbv12I GWGCWC 2 cut(s) 42, 494
BbvI GCAGC 2 cut(s) 43, 436
BccI CCATC 2 cut(s) 141, 263
BceAI ACGGC 1 cut(s) 44
BclI TGATCA 1 cut(s) 657
BcnI CCSGG 1 cut(s) 878
BcoDI GTCTC 1 cut(s) 673
BisI GCNGC 5 cut(s) 57, 148, 428, 450, 864
BlsI GCNGC 5 cut(s) 58, 149, 429, 451, 865
Bme1390I CCNGG 1 cut(s) 878
BmeT110I CYCGRG 2 cut(s) 35, 299
BmrFI CCNGG 1 cut(s) 878
BmrI ACTGGG 1 cut(s) 44
BmsI GCATC 3 cut(s) 461, 535, 751
BmuI ACTGGG 1 cut(s) 44
Bpu14I TTCGAA 1 cut(s) 161
BpuMI CCSGG 1 cut(s) 878
BsaI GGTCTC 1 cut(s) 673
BsaJI CCNNGG 2 cut(s) 298, 666
BsaWI WCCGGW 2 cut(s) 71, 722
Bsc4I CCNNNNNNNGG 1 cut(s) 536
Bse118I RCCGGY 1 cut(s) 143
Bse1I ACTGG 1 cut(s) 50
BseAI TCCGGA 1 cut(s) 722
BseDI CCNNGG 2 cut(s) 298, 666
BseGI GGATG 4 cut(s) 45, 209, 526, 766
BseLI CCNNNNNNNGG 1 cut(s) 536
BseMII CTCAG 1 cut(s) 668
BseNI ACTGG 1 cut(s) 50
BseRI GAGGAG 2 cut(s) 32, 90
BseX3I CGGCCG 2 cut(s) 145, 864
BseXI GCAGC 2 cut(s) 43, 436
BseYI CCCAGC 2 cut(s) 97, 177
Bsh1236I CGCG 1 cut(s) 150
Bsh1285I CGRYCG 2 cut(s) 148, 867
BshFI GGCC 3 cut(s) 147, 155, 866
BsiEI CGRYCG 2 cut(s) 148, 867
BsiHKAI GWGCWC 2 cut(s) 42, 494
BsiHKCI CYCGRG 2 cut(s) 35, 299
BsiSI CCGG 5 cut(s) 72, 144, 723, 867, 877
BslFI GGGAC 1 cut(s) 648
BslI CCNNNNNNNGG 1 cut(s) 536
BsmAI GTCTC 1 cut(s) 673
BsmFI GGGAC 1 cut(s) 648
BsnI GGCC 3 cut(s) 147, 155, 866
Bso31I GGTCTC 1 cut(s) 673
BsoBI CYCGRG 2 cut(s) 35, 299
Bsp119I TTCGAA 1 cut(s) 161
Bsp1286I GDGCHC 2 cut(s) 42, 494
Bsp13I TCCGGA 1 cut(s) 722
Bsp143I GATC 6 cut(s) 31, 303, 484, 516, 657, 869
BspACI CCGC 5 cut(s) 148, 428, 740, 791, 863
BspANI GGCC 3 cut(s) 147, 155, 866
BspCNI CTCAG 1 cut(s) 669
BspEI TCCGGA 1 cut(s) 722
BspFNI CGCG 1 cut(s) 150
BspPI GGATC 3 cut(s) 39, 311, 877
BspQI GCTCTTC 1 cut(s) 795
BspT104I TTCGAA 1 cut(s) 161
BspTNI GGTCTC 1 cut(s) 673
BsrFI RCCGGY 1 cut(s) 143
BsrI ACTGG 1 cut(s) 50
BssAI RCCGGY 1 cut(s) 143
BssECI CCNNGG 2 cut(s) 298, 666
BssMI GATC 6 cut(s) 31, 303, 484, 516, 657, 869
BssT1I CCWWGG 1 cut(s) 666
Bst4CI ACNGT 2 cut(s) 571, 757
Bst6I CTCTTC 2 cut(s) 778, 795
BstBI TTCGAA 1 cut(s) 161
BstDEI CTNAG 2 cut(s) 352, 677
BstF5I GGATG 4 cut(s) 45, 209, 526, 766
BstFNI CGCG 1 cut(s) 150
BstKTI GATC 6 cut(s) 34, 306, 487, 519, 660, 872
BstMAI GTCTC 1 cut(s) 673
BstMBI GATC 6 cut(s) 31, 303, 484, 516, 657, 869
BstMCI CGRYCG 2 cut(s) 148, 867
BstSCI CCNGG 1 cut(s) 876
BstUI CGCG 1 cut(s) 150
BstV1I GCAGC 2 cut(s) 43, 436
BstX2I RGATCY 1 cut(s) 31
BstYI RGATCY 1 cut(s) 31
BstZI CGGCCG 2 cut(s) 145, 864
BsuRI GGCC 3 cut(s) 147, 155, 866
BtsCI GGATG 4 cut(s) 45, 209, 526, 766
CaiI CAGNNNCTG 1 cut(s) 56
Cfr10I RCCGGY 1 cut(s) 143
Csp6I GTAC 1 cut(s) 874
CviAII CATG 2 cut(s) 246, 367
CviQI GTAC 1 cut(s) 874
DdeI CTNAG 2 cut(s) 352, 677
DpnI GATC 6 cut(s) 33, 305, 486, 518, 659, 871
DpnII GATC 6 cut(s) 31, 303, 484, 516, 657, 869
EaeI YGGCCR 2 cut(s) 145, 864
EagI CGGCCG 2 cut(s) 145, 864
Eam1104I CTCTTC 2 cut(s) 778, 795
EarI CTCTTC 2 cut(s) 778, 795
Ecl136II GAGCTC 2 cut(s) 40, 492
EclXI CGGCCG 2 cut(s) 145, 864
Eco130I CCWWGG 1 cut(s) 666
Eco147I AGGCCT 1 cut(s) 155
Eco24I GRGCYC 2 cut(s) 42, 494
Eco31I GGTCTC 1 cut(s) 673
Eco52I CGGCCG 2 cut(s) 145, 864
Eco53kI GAGCTC 2 cut(s) 40, 492
Eco88I CYCGRG 2 cut(s) 35, 299
EcoICRI GAGCTC 2 cut(s) 40, 492
EcoT14I CCWWGG 1 cut(s) 666
EcoT22I ATGCAT 1 cut(s) 550
EcoT38I GRGCYC 2 cut(s) 42, 494
ErhI CCWWGG 1 cut(s) 666
FaeI CATG 2 cut(s) 249, 370
FaqI GGGAC 1 cut(s) 648
FatI CATG 2 cut(s) 245, 366
FbaI TGATCA 1 cut(s) 657
Fnu4HI GCNGC 5 cut(s) 57, 148, 428, 450, 864
FokI GGATG 4 cut(s) 32, 216, 513, 773
FriOI GRGCYC 2 cut(s) 42, 494
Fsp4HI GCNGC 5 cut(s) 57, 148, 428, 450, 864
GluI GCNGC 5 cut(s) 57, 148, 428, 450, 864
GsaI CCCAGC 2 cut(s) 101, 181
HaeIII GGCC 3 cut(s) 147, 155, 866
HapII CCGG 5 cut(s) 72, 144, 723, 867, 877
Hin1II CATG 2 cut(s) 249, 370
HinfI GANTC 1 cut(s) 80
HpaII CCGG 5 cut(s) 72, 144, 723, 867, 877
HphI GGTGA 3 cut(s) 287, 331, 471
Hpy188I TCNGA 5 cut(s) 67, 174, 489, 657, 678
Hpy188III TCNNGA 4 cut(s) 35, 301, 698, 723
HpyAV CCTTC 1 cut(s) 352
HpyCH4III ACNGT 2 cut(s) 571, 757
HpyCH4IV ACGT 2 cut(s) 240, 591
HpyCH4V TGCA 6 cut(s) 370, 452, 548, 764, 838, 918
HpyF3I CTNAG 2 cut(s) 352, 677
HpySE526I ACGT 2 cut(s) 240, 591
Hsp92II CATG 2 cut(s) 249, 370
Kpn2I TCCGGA 1 cut(s) 722
Ksp22I TGATCA 1 cut(s) 657
Kzo9I GATC 6 cut(s) 31, 303, 484, 516, 657, 869
LguI GCTCTTC 1 cut(s) 795
LmnI GCTCC 1 cut(s) 45
Lsp1109I GCAGC 2 cut(s) 43, 436
LweI GCATC 3 cut(s) 461, 535, 751
MaeII ACGT 2 cut(s) 240, 591
MaeIII GTNAC 1 cut(s) 241
MalI GATC 6 cut(s) 33, 305, 486, 518, 659, 871
MboI GATC 6 cut(s) 31, 303, 484, 516, 657, 869
MboII GAAGA 8 cut(s) 206, 209, 268, 340, 611, 795, 812, 902
MflI RGATCY 1 cut(s) 31
MhlI GDGCHC 2 cut(s) 42, 494
MluCI AATT 5 cut(s) 260, 462, 474, 534, 711
Mph1103I ATGCAT 1 cut(s) 550
MroI TCCGGA 1 cut(s) 722
MseI TTAA 4 cut(s) 258, 417, 504, 749
MslI CAYNNNNRTG 1 cut(s) 365
MspI CCGG 5 cut(s) 72, 144, 723, 867, 877
MspR9I CCNGG 1 cut(s) 878
MvnI CGCG 1 cut(s) 150
NciI CCSGG 1 cut(s) 878
NdeII GATC 6 cut(s) 31, 303, 484, 516, 657, 869
NlaIII CATG 2 cut(s) 249, 370
NmuCI GTSAC 1 cut(s) 241
NsiI ATGCAT 1 cut(s) 550
NspV TTCGAA 1 cut(s) 161
PaeR7I CTCGAG 1 cut(s) 35
PceI AGGCCT 1 cut(s) 155
PciSI GCTCTTC 1 cut(s) 795
PfeI GAWTC 1 cut(s) 80
PflMI CCANNNNNTGG 1 cut(s) 536
PkrI GCNGC 5 cut(s) 58, 149, 429, 451, 865
PsiI TTATAA 1 cut(s) 434
Psp124BI GAGCTC 2 cut(s) 42, 494
PspFI CCCAGC 2 cut(s) 97, 177
PstNI CAGNNNCTG 1 cut(s) 56
PsuI RGATCY 1 cut(s) 31
RsaI GTAC 1 cut(s) 875
RsaNI GTAC 1 cut(s) 874
RseI CAYNNNNRTG 1 cut(s) 365
SacI GAGCTC 2 cut(s) 42, 494
SapI GCTCTTC 1 cut(s) 795
SaqAI TTAA 4 cut(s) 258, 417, 504, 749
SatI GCNGC 5 cut(s) 57, 148, 428, 450, 864
Sau3AI GATC 6 cut(s) 31, 303, 484, 516, 657, 869
ScrFI CCNGG 1 cut(s) 878
SduI GDGCHC 2 cut(s) 42, 494
SfaNI GCATC 3 cut(s) 461, 535, 751
Sfr274I CTCGAG 1 cut(s) 35
SfuI TTCGAA 1 cut(s) 161
SlaI CTCGAG 1 cut(s) 35
SmiMI CAYNNNNRTG 1 cut(s) 365
SmlI CTYRAG 1 cut(s) 35
SmoI CTYRAG 1 cut(s) 35
Sse9I AATT 5 cut(s) 260, 462, 474, 534, 711
SseBI AGGCCT 1 cut(s) 155
SsiI CCGC 5 cut(s) 148, 428, 740, 791, 863
SspI AATATT 1 cut(s) 310
SstI GAGCTC 2 cut(s) 42, 494
StuI AGGCCT 1 cut(s) 155
StyD4I CCNGG 1 cut(s) 876
StyI CCWWGG 1 cut(s) 666
TaaI ACNGT 2 cut(s) 571, 757
TaiI ACGT 2 cut(s) 243, 594
TaqI TCGA 4 cut(s) 36, 161, 494, 697
TaqII GACCGA 1 cut(s) 305
TasI AATT 5 cut(s) 260, 462, 474, 534, 711
TauI GCSGC 3 cut(s) 150, 430, 866
TfiI GAWTC 1 cut(s) 80
Tru1I TTAA 4 cut(s) 258, 417, 504, 749
Tru9I TTAA 4 cut(s) 258, 417, 504, 749
TseFI GTSAC 1 cut(s) 241
TseI GCWGC 2 cut(s) 56, 449
Tsp45I GTSAC 1 cut(s) 241
TspDTI ATGAA 3 cut(s) 244, 334, 355
TspGWI ACGGA 1 cut(s) 682
Van91I CCANNNNNTGG 1 cut(s) 536
XapI RAATTY 1 cut(s) 260
XcmI CCANNNNNNNNNTGG 1 cut(s) 638
XhoI CTCGAG 1 cut(s) 35
Zsp2I ATGCAT 1 cut(s) 550
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.