MD15G1031600.v1.1

Protein of unknown function (DUF502)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0015365
Physical Location & Seq
Reverse (-)
1919894 .. 1922344
2451 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1031600.v1.1.491

Sequence Viewer

Length: 939 bp
ATGGCGACGAGAGGGAGAGAAAGGGATCTCGAGCTCCTCATCCCAGTCGCTGCCGTTTCCGAAAACGGAGGAGATTCCAAATCCTCCTCCCCACCAAACTCCCCACCTCCCACCACCTCCCATTCCCATCATCAAACCGGCCACGAGGTTTTCGCATTTTCGAAAGTTATTCGGAGCTGGGCTTCCAAGAAGTTTATGACAGGATGTGTCATTCTTCTTCCAATAGCTATTACGTTCTACATCACATGGGCTTTCATTAAATTTGTTGATGGTTTATTCTCCCCAATCTACAACCACCTAGGAGTCAATATTTTCGGTCTTGGATTTGCCACCTCCCTCGTCTTCATCTTCTTAGTCGGCGTTTTCATGCAGTCGTGGTTGGGAACTTCTGTTCTTACTCTATGGGAGTGGTTTATTACGAAAATGCCACTTATAAGCTATATCTATGCTGCATCAAAGCAAATTAGCACAGCAATATCACCAGATCAGAGCATAAACGCCTTCAAGGAAGTGGCGATCATAAGGCATCCAAGAATCGGGGAGTATGCATTAGGGTTTATCACTTCAACAGTTGTTCTTGATCAAAGAAACGTAGGAGAAGAGCTATGCTGCGTTTATGTACCTACCAACCATCTTTATCTGGGAGACATTTTTCTGATCAGTCCCAAGGATATTCTGAGACCAAATCTGTCAGTTGGAGAGGGGATTGAAATTGTCATCTCAGGAGGTCTGTCAATACCGCAACTTTTAACTACGGTGGATGCACAAGTCCTTACACCCAGAAGAGTTCCCCACTTTGAAGAGCTACAACAGAAGTTGTCAAACTCAAACAAAGTTTTGCAGAGAAAGTTTAGAGGCTCAAACGCGGCCGGATCGGATCGGGAATGGAGGGAAGAACACCATAGCATAACCCTGAAATGCAGTGAAAAGTCCCTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF502

Protein Analysis

313

Amino Acids

34.7

Weight (kDa)

8.31

Isoelectric Point (pI)

40.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF502 PF04367 112 - 211 5.5e-25 Protein of unknown function (DUF502)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015365)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G18460
fragaria_vesca FvH4_2g39890 FvH4_2g39890 FvH4_2g39890
malus_domestica MD08G1034800.v1.1 MD15G1031600.v1.1
prunus_persica Prupe.1G380400_v2.0.a1
pyrus_communis pycom15g02730
rosa_chinensis RchiOBHm_Chr6g0300911
rosa_laevigata RLG00000011291
rosa_multiflora Rmu_sc0006254.1_g000003
rosa_roxburghii Rroxscaffold_7G00167320
rosa_samantha Rh6AG413000 Rh6BG420200 Rh6CG427700 Rh6DG413000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 434
AccII CGCG 1 cut(s) 866
AciI CCGC 2 cut(s) 740, 866
AclWI GGATC 3 cut(s) 33, 880, 885
AcoI YGGCCR 2 cut(s) 139, 867
AcsI RAATTY 1 cut(s) 260
AfaI GTAC 1 cut(s) 621
AfiI CCNNNNNNNGG 1 cut(s) 536
AgsI TTSAA 4 cut(s) 505, 567, 710, 800
AloI GAACNNNNNNTCC 2 cut(s) 375, 407
AluBI AGCT 6 cut(s) 34, 177, 227, 438, 604, 805
AluI AGCT 6 cut(s) 34, 177, 227, 438, 604, 805
Alw21I GWGCWC 1 cut(s) 36
Alw26I GTCTC 2 cut(s) 639, 673
AlwI GGATC 3 cut(s) 33, 880, 885
AlwNI CAGNNNCTG 1 cut(s) 50
Ama87I CYCGRG 1 cut(s) 29
AoxI GGCC 2 cut(s) 139, 867
ApeKI GCWGC 3 cut(s) 50, 449, 609
ApoI RAATTY 1 cut(s) 260
AspA2I CCTAGG 1 cut(s) 298
AsuHPI GGTGA 1 cut(s) 471
AsuII TTCGAA 1 cut(s) 161
AvaI CYCGRG 1 cut(s) 29
AvrII CCTAGG 1 cut(s) 298
BanII GRGCYC 1 cut(s) 36
BauI CACGAG 1 cut(s) 143
BbsI GAAGAC 1 cut(s) 334
Bbv12I GWGCWC 1 cut(s) 36
BbvI GCAGC 3 cut(s) 37, 436, 596
BccI CCATC 3 cut(s) 135, 263, 639
BceAI ACGGC 1 cut(s) 38
BclI TGATCA 2 cut(s) 580, 657
BcoDI GTCTC 2 cut(s) 639, 673
BfaI CTAG 1 cut(s) 299
BisI GCNGC 4 cut(s) 51, 450, 610, 867
BlnI CCTAGG 1 cut(s) 298
BlsI GCNGC 4 cut(s) 52, 451, 611, 868
BmeT110I CYCGRG 1 cut(s) 29
BmrI ACTGGG 1 cut(s) 38
BmsI GCATC 3 cut(s) 461, 535, 751
BmuI ACTGGG 1 cut(s) 38
BpiI GAAGAC 1 cut(s) 334
Bpu14I TTCGAA 1 cut(s) 161
BsaI GGTCTC 1 cut(s) 673
BsaJI CCNNGG 2 cut(s) 298, 666
Bsc4I CCNNNNNNNGG 1 cut(s) 536
Bse118I RCCGGY 1 cut(s) 137
Bse1I ACTGG 1 cut(s) 44
BseDI CCNNGG 2 cut(s) 298, 666
BseGI GGATG 4 cut(s) 39, 209, 526, 766
BseLI CCNNNNNNNGG 1 cut(s) 536
BseMII CTCAG 2 cut(s) 668, 735
BseNI ACTGG 1 cut(s) 44
BseRI GAGGAG 3 cut(s) 26, 76, 84
BseX3I CGGCCG 1 cut(s) 867
BseXI GCAGC 3 cut(s) 37, 436, 596
BseYI CCCAGC 1 cut(s) 177
Bsh1236I CGCG 1 cut(s) 866
Bsh1285I CGRYCG 1 cut(s) 870
BshFI GGCC 2 cut(s) 141, 869
BsiEI CGRYCG 1 cut(s) 870
BsiHKAI GWGCWC 1 cut(s) 36
BsiHKCI CYCGRG 1 cut(s) 29
BsiSI CCGG 2 cut(s) 138, 870
BslFI GGGAC 2 cut(s) 648, 916
BslI CCNNNNNNNGG 1 cut(s) 536
BsmAI GTCTC 2 cut(s) 639, 673
BsmFI GGGAC 2 cut(s) 648, 916
BsnI GGCC 2 cut(s) 141, 869
Bso31I GGTCTC 1 cut(s) 673
BsoBI CYCGRG 1 cut(s) 29
Bsp119I TTCGAA 1 cut(s) 161
Bsp1286I GDGCHC 1 cut(s) 36
Bsp143I GATC 7 cut(s) 25, 484, 516, 580, 657, 872, 877
BspACI CCGC 2 cut(s) 740, 866
BspANI GGCC 2 cut(s) 141, 869
BspCNI CTCAG 2 cut(s) 669, 734
BspFNI CGCG 1 cut(s) 866
BspPI GGATC 3 cut(s) 33, 880, 885
BspQI GCTCTTC 2 cut(s) 594, 795
BspT104I TTCGAA 1 cut(s) 161
BspTNI GGTCTC 1 cut(s) 673
BsrFI RCCGGY 1 cut(s) 137
BsrI ACTGG 1 cut(s) 44
BssAI RCCGGY 1 cut(s) 137
BssECI CCNNGG 2 cut(s) 298, 666
BssMI GATC 7 cut(s) 25, 484, 516, 580, 657, 872, 877
BssSI CACGAG 1 cut(s) 143
BssT1I CCWWGG 2 cut(s) 298, 666
Bst2BI CACGAG 1 cut(s) 143
Bst4CI ACNGT 2 cut(s) 571, 757
Bst6I CTCTTC 3 cut(s) 594, 778, 795
BstBI TTCGAA 1 cut(s) 161
BstDEI CTNAG 3 cut(s) 352, 677, 721
BstF5I GGATG 4 cut(s) 39, 209, 526, 766
BstFNI CGCG 1 cut(s) 866
BstKTI GATC 7 cut(s) 28, 487, 519, 583, 660, 875, 880
BstMAI GTCTC 2 cut(s) 639, 673
BstMBI GATC 7 cut(s) 25, 484, 516, 580, 657, 872, 877
BstMCI CGRYCG 1 cut(s) 870
BstUI CGCG 1 cut(s) 866
BstV1I GCAGC 3 cut(s) 37, 436, 596
BstV2I GAAGAC 1 cut(s) 334
BstX2I RGATCY 1 cut(s) 25
BstYI RGATCY 1 cut(s) 25
BstZI CGGCCG 1 cut(s) 867
BsuRI GGCC 2 cut(s) 141, 869
BtsCI GGATG 4 cut(s) 39, 209, 526, 766
BtsI GCAGTG 1 cut(s) 928
BtsIMutI CAGTG 1 cut(s) 928
CaiI CAGNNNCTG 1 cut(s) 50
Cfr10I RCCGGY 1 cut(s) 137
Csp6I GTAC 1 cut(s) 620
CviAII CATG 2 cut(s) 246, 367
CviQI GTAC 1 cut(s) 620
DdeI CTNAG 3 cut(s) 352, 677, 721
DpnI GATC 7 cut(s) 27, 486, 518, 582, 659, 874, 879
DpnII GATC 7 cut(s) 25, 484, 516, 580, 657, 872, 877
EaeI YGGCCR 2 cut(s) 139, 867
EagI CGGCCG 1 cut(s) 867
Eam1104I CTCTTC 3 cut(s) 594, 778, 795
EarI CTCTTC 3 cut(s) 594, 778, 795
Ecl136II GAGCTC 1 cut(s) 34
EclXI CGGCCG 1 cut(s) 867
Eco130I CCWWGG 2 cut(s) 298, 666
Eco24I GRGCYC 1 cut(s) 36
Eco31I GGTCTC 1 cut(s) 673
Eco52I CGGCCG 1 cut(s) 867
Eco53kI GAGCTC 1 cut(s) 34
Eco88I CYCGRG 1 cut(s) 29
EcoICRI GAGCTC 1 cut(s) 34
EcoT14I CCWWGG 2 cut(s) 298, 666
EcoT22I ATGCAT 1 cut(s) 550
EcoT38I GRGCYC 1 cut(s) 36
ErhI CCWWGG 2 cut(s) 298, 666
FaeI CATG 2 cut(s) 249, 370
FaqI GGGAC 2 cut(s) 648, 916
FatI CATG 2 cut(s) 245, 366
FbaI TGATCA 2 cut(s) 580, 657
Fnu4HI GCNGC 4 cut(s) 51, 450, 610, 867
FokI GGATG 4 cut(s) 26, 216, 513, 773
FriOI GRGCYC 1 cut(s) 36
Fsp4HI GCNGC 4 cut(s) 51, 450, 610, 867
FspBI CTAG 1 cut(s) 299
GluI GCNGC 4 cut(s) 51, 450, 610, 867
GsaI CCCAGC 1 cut(s) 181
HaeIII GGCC 2 cut(s) 141, 869
HapII CCGG 2 cut(s) 138, 870
Hin1II CATG 2 cut(s) 249, 370
HinfI GANTC 3 cut(s) 74, 303, 534
HpaII CCGG 2 cut(s) 138, 870
HphI GGTGA 1 cut(s) 471
Hpy188I TCNGA 6 cut(s) 61, 174, 489, 657, 678, 877
Hpy188III TCNNGA 4 cut(s) 29, 578, 723, 881
Hpy99I CGWCG 1 cut(s) 10
HpyAV CCTTC 1 cut(s) 511
HpyCH4III ACNGT 2 cut(s) 571, 757
HpyCH4IV ACGT 2 cut(s) 233, 591
HpyCH4V TGCA 6 cut(s) 370, 452, 548, 764, 841, 921
HpyF3I CTNAG 3 cut(s) 352, 677, 721
HpySE526I ACGT 2 cut(s) 233, 591
Hsp92II CATG 2 cut(s) 249, 370
Ksp22I TGATCA 2 cut(s) 580, 657
Kzo9I GATC 7 cut(s) 25, 484, 516, 580, 657, 872, 877
LguI GCTCTTC 2 cut(s) 594, 795
LmnI GCTCC 2 cut(s) 39, 174
Lsp1109I GCAGC 3 cut(s) 37, 436, 596
LweI GCATC 3 cut(s) 461, 535, 751
MaeI CTAG 1 cut(s) 299
MaeII ACGT 2 cut(s) 233, 591
MalI GATC 7 cut(s) 27, 486, 518, 582, 659, 874, 879
MboI GATC 7 cut(s) 25, 484, 516, 580, 657, 872, 877
MboII GAAGA 8 cut(s) 206, 209, 334, 340, 611, 795, 812, 905
MflI RGATCY 1 cut(s) 25
MhlI GDGCHC 1 cut(s) 36
MluCI AATT 3 cut(s) 260, 462, 711
MlyI GAGTC 1 cut(s) 312
MmeI TCCRAC 1 cut(s) 676
Mph1103I ATGCAT 1 cut(s) 550
MseI TTAA 2 cut(s) 258, 749
MspI CCGG 2 cut(s) 138, 870
MvnI CGCG 1 cut(s) 866
NdeII GATC 7 cut(s) 25, 484, 516, 580, 657, 872, 877
NlaIII CATG 2 cut(s) 249, 370
NsiI ATGCAT 1 cut(s) 550
NspV TTCGAA 1 cut(s) 161
PaeR7I CTCGAG 1 cut(s) 29
PciSI GCTCTTC 2 cut(s) 594, 795
PfeI GAWTC 2 cut(s) 74, 534
PkrI GCNGC 4 cut(s) 52, 451, 611, 868
PleI GAGTC 1 cut(s) 311
PpsI GAGTC 1 cut(s) 311
PsiI TTATAA 1 cut(s) 434
Psp124BI GAGCTC 1 cut(s) 36
PspFI CCCAGC 1 cut(s) 177
PstNI CAGNNNCTG 1 cut(s) 50
PsuI RGATCY 1 cut(s) 25
RsaI GTAC 1 cut(s) 621
RsaNI GTAC 1 cut(s) 620
SacI GAGCTC 1 cut(s) 36
SapI GCTCTTC 2 cut(s) 594, 795
SaqAI TTAA 2 cut(s) 258, 749
SatI GCNGC 4 cut(s) 51, 450, 610, 867
Sau3AI GATC 7 cut(s) 25, 484, 516, 580, 657, 872, 877
SchI GAGTC 1 cut(s) 312
SduI GDGCHC 1 cut(s) 36
SfaNI GCATC 3 cut(s) 461, 535, 751
Sfr274I CTCGAG 1 cut(s) 29
SfuI TTCGAA 1 cut(s) 161
SlaI CTCGAG 1 cut(s) 29
SmlI CTYRAG 1 cut(s) 29
SmoI CTYRAG 1 cut(s) 29
Sse9I AATT 3 cut(s) 260, 462, 711
SsiI CCGC 2 cut(s) 740, 866
SspI AATATT 1 cut(s) 310
SspMI CTAG 1 cut(s) 299
SstI GAGCTC 1 cut(s) 36
StyI CCWWGG 2 cut(s) 298, 666
TaaI ACNGT 2 cut(s) 571, 757
TaiI ACGT 2 cut(s) 236, 594
TaqI TCGA 2 cut(s) 30, 161
TaqII GACCGA 1 cut(s) 305
TasI AATT 3 cut(s) 260, 462, 711
TauI GCSGC 1 cut(s) 869
TfiI GAWTC 2 cut(s) 74, 534
Tru1I TTAA 2 cut(s) 258, 749
Tru9I TTAA 2 cut(s) 258, 749
TscAI CASTG 1 cut(s) 928
TseI GCWGC 3 cut(s) 50, 449, 609
TspDTI ATGAA 3 cut(s) 244, 334, 355
TspGWI ACGGA 1 cut(s) 81
TspRI CASTG 1 cut(s) 928
XapI RAATTY 1 cut(s) 260
XhoI CTCGAG 1 cut(s) 29
XmaJI CCTAGG 1 cut(s) 298
XspI CTAG 1 cut(s) 299
Zsp2I ATGCAT 1 cut(s) 550
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.