Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr08

Orthogroup ID

OG0009276

Physical Location & Seq

Genomic Coordinates Reverse (-)

4281898 .. 4294234

Gene Structure 12337 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD08G1054700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2958 bp

ATGAGTTCGTTCTCGATTACGCGGAAGAAGACTCCGTTCCAGAAGCACAGGGAGGAGGAAGAGGCAAAGAAAAAGAGAGCGGAGGATGAAACAGCTCGCTTATATGCGGAGTTTGTGGAGTCATTTCAAGGGGATAACGCACCGGGGTCGAAGGCCTTTGTTCGTGGAGGAACAATCAATCCCAATGAGAAGGTGAAGCCGGATTCCGAAGGTGAAAAGTCCAAAGATGGGGTATCTGTTCCAAAGAAGGGGAGTAGGTATGTTCCATCTTTCTTACCACCACCTTTGGCATCCAAAGGGAAAGAATCCGAGAAGAAGAGGGAGGAGGAGAGACCGAGGGAGAAAGAAAAAGGAAAGTCTCGAAACATAGATCATTTTATGGAAGAGTTGAAGCGTGAGCAGGAGATGAGGGAGAGGCGAAATCAAGACCGTGAAAATTGGCATGATGGGCGGCAGATTGAGAATTCTACACCATCCAGTCGCTTTGATGAACTTCCTGATGAATTTGATCCAAGTGGAAAGCTTCTTGGATCATTTGATGATGGTGATCCACAAACTACTAATCTATACGTTGGAAATCTTTCGCCAAAGGTTGATGAAAATTTTCTTTTGCGAACTTTTGGAAGATTTGGGCCTATTGCTAGTGTGAAAATAATGTGGCCTAGGACAGAAGAGGAGCGAAGGCGCCAAAGAAATTGTGGCTTTGTAGCTTTCATGAACAGAGCTGATGGACAGGCAGCGAAAGATGAAATGCAAGGAGTGGTGGTTTATGAGTATGAGTTGAAGATTGGGTGGGGCAAGTCTGTTGCTCTACCATCACAAGCTCTACCTGCTCCCCCTCCAGGACATATGGCCATCAGGAGTGAGGGTTCCACTGTAATCTTGTCTGGTCCATCAGGTTCATCAGGCCCGCCTGTCACTTCTGTTCCTAGTCAGAATTCTGAACTGGTTCTTACTCCAAATGTTCCTGATATAACCGTTGTTCCACCAGAGGATGATCACCTCCGCCATGTGATTGATACAATGGCTTTGTATGTTCTGGATGGTGGATGTTCCTTTGAACAAGCTATTATGGAGAGAGGTAGAGGGAATCCTCTTTTCAACTTCTTGTTTGAACTTGGCTCAAAAGAGCACACCTACTATGTTTGGAGACTCTATTCATTTGCTCAGGGTGATACTCTACAAAGGTGGCGAACAGAACCTTTCATTATGATTACTGGTAGTGGAAGATGGATTCCACCACCTCTGCCAACAGTAAAAAGCCCAGAACATGAGAAAGAGGCCGGTACAACATATGCTGCAGGAAGAAGCAGGCGTGTAGAGCCAGAAAGAACACTTACTGATTCACAGAGGGATGAATTTGAAGATATGTTACGTGCATTAACATTAGAGAGGAGTCAGATAAAGGATGCTATGGGATTTGCATTGGACAATGCTGATGCAGCTGGGGAGATAGTTGAAGTTCTTACAGAATCTTTGACTCTTAAGGAGACCCCTATCCCAACCAAAGTAGCAAGACTCATGCTTGTCTCTGATGTGCTTCACAATAGCAGTGCTCCGGTAAAGAATGCATCTGCATACCGCACCAAATTTGAAGCTTCATTACCAGACATAATGGAGAGCTTTAATGATTTGTATCGTAGCATAACAGGAAGGATCACTGCTGAGGCCCTTAAGGAAAGAGTTCTGAAAGTATTGCAAGTATGGTCGGACTGGTTTCTTTTTTCAGACGCTTACGTGAATGGATTACGGGCTACTTTTCTTCGGTCTGGGAACTCTCATGTGGTCCCCTTTCACTCCATATGTGGTGAAGCACCTGAAATAGACAAAAAGACCACTTCCGAAGATACAAGTGATGCTAGTAAAACCAATCAAGATGCTGCTTTGGCAATGGGCAAAGGAGCAGCTATGAGGGAGTTAATGAGTCTTCCCCTAGCTGAGTTGGAAAGACGCTGCAGACATAATGGATTGTCTCTTGTTGGTGGTCGAGAAACAATGGTTGCACGTTTGCTTAGTCTTGAAGAAGCAGAAAAACAAAGGGGCTACGACTTAGATGATGACTTGAAATTTGCTCAGAAGCATTCAAGTTCAGCTAGAAATTCAAGTAGCCGCAGAGAAAGGAATATTGAACCAGACCCAGTGGAAATGTCTGGATGGAATCGTAATGTAGAGGATGAGTTACAGTCTCAAGGGAAGGGTTCCCTACCCTTGGCCCAGACCTTTCCCATCCCGCATCCTGAACTAAATGCGTTTACAAAGAAAGAGAAGAGTGACCCTATTCTACCAGCTTCTAAATGGGCGCGAGAGGATGATGATAGTGATGACGAACAAAAGCAGGCTCGGGGTCTCGGGTTAAGCTACTCATCTTCCGGAAGTGAAAATGCTGGTGATGGTCCCAGTAAAGCTGATGAGACGGAGGTCGCAACTGATGCAAGCATTCAAGCACAACCTGACAGTGGAATTAGTGAGGAGCAGAGGCAAAAGTTGAGACGTTTGGAAGTCGCTTTGATAGAATATCGTGAATCTCTTGAGGAGCGGGGAATAAAAAATCTTGAAGAAATTGAGAGGAAGGTTTTAATGTATCGGAAACGGCTTGAATCAGAATATGGGTTATCAGATTCCAGTGAATATGCTTCCGGAAGTAAGAGAACATCCTCAGAGAGGGACAGACGGGATGCCGATACTCGTGATGCTTCAAGAAAGCGGCACCGCAGTGGAAGCCAAGGTGATAGTCCACTACAAAGATCATCAAACAGAGATCGGGAAAGGGAAAATGATTTAGACAGAGACCGGGAAAGGCAGCGGGATAGAAACAGAGACAGACCCCGTGATTTTGAAGTTGATAGGGGGAGGGGCCGGGACCTGGATCGTGAGAAGAGTGGAAGTAGAGACAGGGATGACCATGAGAGAGATAGAAGCAGAGAAGGAGACAGGGATAGGAGGAGAAGAATGAAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0031974 GO:0031981 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0070013

KEGG Pathways

Metabolic & Signaling

ko03040 map03040

Pfam Domains

Protein Families

RRM_1 Surp cwf21

Protein Analysis

986

Amino Acids

111.63

Weight (kDa)

5.86

Isoelectric Point (pI)

55.16

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
RRM_1	PF00076	189 - 261	9.1e-11	RNA recognition motif
Surp	PF01805	336 - 388	7.7e-16	Surp module
CID	PF04818	453 - 581	8.5e-08	CID domain
cwf21	PF08312	824 - 868	3.7e-06	cwf21 domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009276)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G10800 AT5G25060
fragaria_vesca	FvH4_2g41230 FvH4_2g41230 FvH4_2g41230
malus_domestica	MD08G1054700.v1.1 MD15G1042300.v1.1
prunus_persica	Prupe.1G395500_v2.0.a1 Prupe.1G395500_v2.0.a1 Prupe.1G395500_v2.0.a1 Prupe.1G395500_v2.0.a1
pyrus_communis	pycom08g04510 pycom15g03940
rosa_chinensis	RchiOBHm_Chr6g0299281
rosa_laevigata	RLG00000011446
rosa_roxburghii	Rroxscaffold_7G00168830
rosa_rugosa	Rorug06G0286800
rosa_samantha	Rh6AG398300 Rh6BG406700 Rh6CG412800 Rh6DG399500
rosa_wichuraiana	Rw6G034860

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	838
AccB1I	GGYRCC	2 cut(s)	684, 2706
AccBSI	CCGCTC	2 cut(s)	80, 2536
AccII	CGCG	2 cut(s)	22, 2302
AccIII	TCCGGA	2 cut(s)	2369, 2636
AclWI	GGATC	5 cut(s)	503, 538, 542, 1664, 2874
AcoI	YGGCCR	1 cut(s)	852
AcsI	RAATTY	8 cut(s)	463, 503, 601, 937, 1358, 1589, 2066, 2098
AcyI	GRCGYC	1 cut(s)	685
AfaI	GTAC	1 cut(s)	1288
AfiI	CCNNNNNNNGG	7 cut(s)	228, 248, 842, 2209, 2456, 2661, 2863
AflII	CTTAAG	2 cut(s)	1484, 1673
AjnI	CCWGG	2 cut(s)	841, 2862
AleI	CACNNNNGTG	1 cut(s)	2712
AloI	GAACNNNNNNTCC	4 cut(s)	163, 195, 853, 885
Alw21I	GWGCWC	2 cut(s)	1134, 1558
AlwI	GGATC	5 cut(s)	503, 538, 542, 1664, 2874
Ama87I	CYCGRG	2 cut(s)	2340, 2348
Aor13HI	TCCGGA	2 cut(s)	2369, 2636
AoxI	GGCC	9 cut(s)	153, 632, 659, 852, 907, 1281, 1668, 2211, 2854
ApeKI	GCWGC	7 cut(s)	737, 1298, 1442, 1880, 1904, 1953, 2800
ApoI	RAATTY	8 cut(s)	463, 503, 601, 937, 1358, 1589, 2066, 2098
ArsI	GACNNNNNNTTYG	2 cut(s)	2051, 2083
Asp700I	GAANNNNTTC	4 cut(s)	580, 603, 948, 1683
AspA2I	CCTAGG	1 cut(s)	662
AspLEI	GCGC	2 cut(s)	687, 2302
AspS9I	GGNCC	9 cut(s)	632, 890, 908, 1669, 1786, 2212, 2393, 2854, 2860
AsuC2I	CCSGG	3 cut(s)	144, 2792, 2858
AsuHPI	GGTGA	8 cut(s)	205, 224, 557, 992, 1184, 1820, 2399, 2738
AvaI	CYCGRG	2 cut(s)	2340, 2348
AvaII	GGWCC	4 cut(s)	890, 1786, 2393, 2860
AvrII	CCTAGG	1 cut(s)	662
BalI	TGGCCA	1 cut(s)	854
BanI	GGYRCC	2 cut(s)	684, 2706
BarI	GAAGNNNNNNTAC	4 cut(s)	1356, 1388, 1587, 1619
BauI	CACGAG	1 cut(s)	2685
BbsI	GAAGAC	2 cut(s)	35, 1919
Bbv12I	GWGCWC	2 cut(s)	1134, 1558
BbvCI	CCTCAGC	1 cut(s)	1665
BbvI	GCAGC	7 cut(s)	749, 1285, 1454, 1867, 1916, 1940, 2812
BceAI	ACGGC	1 cut(s)	2606
BcgI	CGANNNNNNTGC	2 cut(s)	129, 163
BciT130I	CCWGG	2 cut(s)	843, 2864
BclI	TGATCA	1 cut(s)	997
BcnI	CCSGG	3 cut(s)	144, 2792, 2858
BfaI	CTAG	6 cut(s)	642, 663, 930, 1860, 1934, 2094
BfmI	CTRYAG	2 cut(s)	1299, 1954
BfoI	RGCGCY	1 cut(s)	688
BfrI	CTTAAG	2 cut(s)	1484, 1673
BfuAI	ACCTGC	1 cut(s)	838
BlnI	CCTAGG	1 cut(s)	662
Bme1390I	CCNGG	5 cut(s)	144, 843, 2792, 2858, 2864
Bme18I	GGWCC	4 cut(s)	890, 1786, 2393, 2860
BmeT110I	CYCGRG	2 cut(s)	2340, 2348
BmgT120I	GGNCC	9 cut(s)	632, 890, 908, 1669, 1786, 2212, 2393, 2854, 2860
BmiI	GGNNCC	8 cut(s)	686, 871, 1788, 2200, 2395, 2708, 2855, 2861
BmrFI	CCNGG	5 cut(s)	144, 843, 2792, 2858, 2864
BmrI	ACTGGG	2 cut(s)	2132, 2391
BmuI	ACTGGG	2 cut(s)	2132, 2391
BoxI	GACNNNNGTC	1 cut(s)	2417
BpiI	GAAGAC	2 cut(s)	35, 1919
BpmI	CTGGAG	1 cut(s)	825
Bpu10I	CCTNAGC	2 cut(s)	1167, 1665
BpuEI	CTTGAG	2 cut(s)	2172, 2549
BpuMI	CCSGG	3 cut(s)	144, 2792, 2858
BsaAI	YACGTR	2 cut(s)	1376, 1738
BsaBI	GATNNNNATC	2 cut(s)	546, 1635
BsaHI	GRCGYC	1 cut(s)	685
BsaI	GGTCTC	4 cut(s)	325, 1484, 2351, 2781
BsaJI	CCNNGG	5 cut(s)	143, 335, 662, 2208, 2722
BsaWI	WCCGGW	3 cut(s)	1558, 2369, 2636
BsaXI	ACNNNNNCTCC	4 cut(s)	750, 780, 853, 883
Bsc4I	CCNNNNNNNGG	7 cut(s)	228, 248, 842, 2209, 2456, 2661, 2863
Bse118I	RCCGGY	1 cut(s)	1283
Bse1I	ACTGG	7 cut(s)	477, 951, 1222, 1718, 2138, 2397, 2622
Bse3DI	GCAATG	1 cut(s)	1896
Bse8I	GATNNNNATC	2 cut(s)	546, 1635
BseAI	TCCGGA	2 cut(s)	2369, 2636
BseBI	CCWGG	2 cut(s)	843, 2864
BseDI	CCNNGG	5 cut(s)	143, 335, 662, 2208, 2722
BseJI	GATNNNNATC	2 cut(s)	546, 1635
BseLI	CCNNNNNNNGG	7 cut(s)	228, 248, 842, 2209, 2456, 2661, 2863
BseMI	GCAATG	1 cut(s)	1896
BseMII	CTCAG	5 cut(s)	1181, 1656, 1929, 2087, 2670
BseNI	ACTGG	7 cut(s)	477, 951, 1222, 1718, 2138, 2397, 2622
BseRI	GAGGAG	8 cut(s)	68, 338, 341, 689, 1408, 2483, 2546, 2956
BseXI	GCAGC	7 cut(s)	749, 1285, 1454, 1867, 1916, 1940, 2812
BseYI	CCCAGC	1 cut(s)	1445
Bsh1236I	CGCG	2 cut(s)	22, 2302
BshFI	GGCC	9 cut(s)	155, 634, 661, 854, 909, 1283, 1670, 2213, 2856
BshNI	GGYRCC	2 cut(s)	684, 2706
BsiHKAI	GWGCWC	2 cut(s)	1134, 1558
BsiHKCI	CYCGRG	2 cut(s)	2340, 2348
BsiSI	CCGG	8 cut(s)	143, 200, 1284, 1559, 2370, 2637, 2791, 2857
BslFI	GGGAC	4 cut(s)	1772, 2379, 2678, 2873
BslI	CCNNNNNNNGG	7 cut(s)	228, 248, 842, 2209, 2456, 2661, 2863
BsmBI	CGTCTC	2 cut(s)	2405, 2482
BsmFI	GGGAC	4 cut(s)	1772, 2379, 2678, 2873
BsmI	GAATGC	3 cut(s)	1573, 2080, 2436
BsnI	GGCC	9 cut(s)	155, 634, 661, 854, 909, 1283, 1670, 2213, 2856
Bso31I	GGTCTC	4 cut(s)	325, 1484, 2351, 2781
BsoBI	CYCGRG	2 cut(s)	2340, 2348
Bsp1286I	GDGCHC	2 cut(s)	1134, 1558
Bsp13I	TCCGGA	2 cut(s)	2369, 2636
Bsp143I	GATC	9 cut(s)	370, 508, 530, 547, 997, 1656, 2744, 2758, 2866
BspANI	GGCC	9 cut(s)	155, 634, 661, 854, 909, 1283, 1670, 2213, 2856
BspCNI	CTCAG	5 cut(s)	1180, 1657, 1930, 2086, 2669
BspEI	TCCGGA	2 cut(s)	2369, 2636
BspFNI	CGCG	2 cut(s)	22, 2302
BspHI	TCATGA	1 cut(s)	714
BspLI	GGNNCC	8 cut(s)	686, 871, 1788, 2200, 2395, 2708, 2855, 2861
BspMAI	CTGCAG	2 cut(s)	1303, 1958
BspMI	ACCTGC	1 cut(s)	838
BspPI	GGATC	5 cut(s)	503, 538, 542, 1664, 2874
BspT107I	GGYRCC	2 cut(s)	684, 2706
BspTI	CTTAAG	2 cut(s)	1484, 1673
BspTNI	GGTCTC	4 cut(s)	325, 1484, 2351, 2781
BsrBI	CCGCTC	2 cut(s)	80, 2536
BsrDI	GCAATG	1 cut(s)	1896
BsrFI	RCCGGY	1 cut(s)	1283
BsrI	ACTGG	7 cut(s)	477, 951, 1222, 1718, 2138, 2397, 2622
BssAI	RCCGGY	1 cut(s)	1283
BssECI	CCNNGG	5 cut(s)	143, 335, 662, 2208, 2722
BssMI	GATC	9 cut(s)	370, 508, 530, 547, 997, 1656, 2744, 2758, 2866
BssNI	GRCGYC	1 cut(s)	685
BssSI	CACGAG	1 cut(s)	2685
BssT1I	CCWWGG	3 cut(s)	662, 2208, 2722
Bst2BI	CACGAG	1 cut(s)	2685
Bst2UI	CCWGG	2 cut(s)	843, 2864
Bst4CI	ACNGT	6 cut(s)	431, 877, 979, 1255, 2184, 2456
Bst6I	CTCTTC	6 cut(s)	54, 311, 378, 666, 2261, 2870
BstACI	GRCGYC	1 cut(s)	685
BstAFI	CTTAAG	2 cut(s)	1484, 1673
BstAPI	GCANNNNNTGC	1 cut(s)	2429
BstBAI	YACGTR	2 cut(s)	1376, 1738
BstC8I	GCNNGC	5 cut(s)	97, 911, 1313, 2337, 2434
BstDEI	CTNAG	7 cut(s)	1167, 1665, 1938, 2012, 2050, 2073, 2656
BstENI	CCTNNNNNAGG	1 cut(s)	2659
BstFNI	CGCG	2 cut(s)	22, 2302
BstH2I	RGCGCY	1 cut(s)	688
BstHHI	GCGC	2 cut(s)	687, 2302
BstKTI	GATC	9 cut(s)	373, 511, 533, 550, 1000, 1659, 2747, 2761, 2869
BstMBI	GATC	9 cut(s)	370, 508, 530, 547, 997, 1656, 2744, 2758, 2866
BstMWI	GCNNNNNNNGC	7 cut(s)	448, 830, 1321, 1442, 1886, 2429, 2718
BstNI	CCWGG	2 cut(s)	843, 2864
BstPAI	GACNNNNGTC	1 cut(s)	2417
BstSCI	CCNGG	5 cut(s)	142, 841, 2790, 2856, 2862
BstSFI	CTRYAG	2 cut(s)	1299, 1954
BstUI	CGCG	2 cut(s)	22, 2302
BstV1I	GCAGC	7 cut(s)	749, 1285, 1454, 1867, 1916, 1940, 2812
BstV2I	GAAGAC	2 cut(s)	35, 1919
BsuRI	GGCC	9 cut(s)	155, 634, 661, 854, 909, 1283, 1670, 2213, 2856
BtsI	GCAGTG	3 cut(s)	1558, 1659, 2719
BtsIMutI	CAGTG	7 cut(s)	873, 1558, 1659, 2145, 2461, 2629, 2719
BveI	ACCTGC	1 cut(s)	838
Cac8I	GCNNGC	5 cut(s)	97, 911, 1313, 2337, 2434
CciI	TCATGA	1 cut(s)	714
CfoI	GCGC	2 cut(s)	687, 2302
Cfr10I	RCCGGY	1 cut(s)	1283
Cfr13I	GGNCC	9 cut(s)	632, 890, 908, 1669, 1786, 2212, 2393, 2854, 2860
CseI	GACGC	2 cut(s)	1739, 1959
Csp6I	GTAC	1 cut(s)	1287
CspCI	CAANNNNNGTGG	2 cut(s)	267, 302
CviAII	CATG	7 cut(s)	443, 715, 1010, 1271, 1522, 1781, 2903
CviQI	GTAC	1 cut(s)	1287
DdeI	CTNAG	7 cut(s)	1167, 1665, 1938, 2012, 2050, 2073, 2656
DinI	GGCGCC	1 cut(s)	686
DpnI	GATC	9 cut(s)	372, 510, 532, 549, 999, 1658, 2746, 2760, 2868
DpnII	GATC	9 cut(s)	370, 508, 530, 547, 997, 1656, 2744, 2758, 2866
EaeI	YGGCCR	1 cut(s)	852
Eam1104I	CTCTTC	6 cut(s)	54, 311, 378, 666, 2261, 2870
EarI	CTCTTC	6 cut(s)	54, 311, 378, 666, 2261, 2870
EciI	GGCGGA	1 cut(s)	995
Eco130I	CCWWGG	3 cut(s)	662, 2208, 2722
Eco147I	AGGCCT	1 cut(s)	155
Eco31I	GGTCTC	4 cut(s)	325, 1484, 2351, 2781
Eco47I	GGWCC	4 cut(s)	890, 1786, 2393, 2860
Eco88I	CYCGRG	2 cut(s)	2340, 2348
EcoNI	CCTNNNNNAGG	1 cut(s)	2659
EcoO109I	RGGNCCY	2 cut(s)	1669, 2860
EcoRI	GAATTC	2 cut(s)	463, 937
EcoRII	CCWGG	2 cut(s)	841, 2862
EcoT14I	CCWWGG	3 cut(s)	662, 2208, 2722
EcoT22I	ATGCAT	1 cut(s)	1573
EgeI	GGCGCC	1 cut(s)	686
EheI	GGCGCC	1 cut(s)	686
ErhI	CCWWGG	3 cut(s)	662, 2208, 2722
Esp3I	CGTCTC	2 cut(s)	2405, 2482
FaeI	CATG	7 cut(s)	446, 718, 1013, 1274, 1525, 1784, 2906
FaqI	GGGAC	4 cut(s)	1772, 2379, 2678, 2873
FatI	CATG	7 cut(s)	442, 714, 1009, 1270, 1521, 1780, 2902
FauI	CCCGC	4 cut(s)	918, 2238, 2529, 2796
FauNDI	CATATG	3 cut(s)	849, 1294, 1802
FbaI	TGATCA	1 cut(s)	997
FspBI	CTAG	6 cut(s)	642, 663, 930, 1860, 1934, 2094
GlaI	GCGC	2 cut(s)	686, 2301
GsaI	CCCAGC	1 cut(s)	1449
GsuI	CTGGAG	1 cut(s)	825
HaeII	RGCGCY	1 cut(s)	688
HaeIII	GGCC	9 cut(s)	155, 634, 661, 854, 909, 1283, 1670, 2213, 2856
HapII	CCGG	8 cut(s)	143, 200, 1284, 1559, 2370, 2637, 2791, 2857
HgaI	GACGC	2 cut(s)	1739, 1959
HhaI	GCGC	2 cut(s)	687, 2302
Hin1I	GRCGYC	1 cut(s)	685
Hin1II	CATG	7 cut(s)	446, 718, 1013, 1274, 1525, 1784, 2906
Hin6I	GCGC	2 cut(s)	685, 2300
HinP1I	GCGC	2 cut(s)	685, 2300
HindIII	AAGCTT	2 cut(s)	521, 1596
HpaII	CCGG	8 cut(s)	143, 200, 1284, 1559, 2370, 2637, 2791, 2857
HphI	GGTGA	8 cut(s)	205, 224, 557, 992, 1184, 1820, 2399, 2738
Hpy166II	GTNNAC	2 cut(s)	2253, 2735
Hpy8I	GTNNAC	2 cut(s)	2253, 2735
HpyAV	CCTTC	9 cut(s)	145, 184, 203, 241, 675, 1647, 2188, 2563, 2918
HpyCH4III	ACNGT	6 cut(s)	431, 877, 979, 1255, 2184, 2456
HpyCH4IV	ACGT	5 cut(s)	570, 1375, 1737, 2005, 2491
HpyF10VI	GCNNNNNNNGC	7 cut(s)	448, 830, 1321, 1442, 1886, 2429, 2718
HpyF3I	CTNAG	7 cut(s)	1167, 1665, 1938, 2012, 2050, 2073, 2656
HpySE526I	ACGT	5 cut(s)	570, 1375, 1737, 2005, 2491
Hsp92I	GRCGYC	1 cut(s)	685
Hsp92II	CATG	7 cut(s)	446, 718, 1013, 1274, 1525, 1784, 2906
HspAI	GCGC	2 cut(s)	685, 2300
KasI	GGCGCC	1 cut(s)	684
Kpn2I	TCCGGA	2 cut(s)	2369, 2636
Ksp22I	TGATCA	1 cut(s)	997
Kzo9I	GATC	9 cut(s)	370, 508, 530, 547, 997, 1656, 2744, 2758, 2866
LmnI	GCTCC	6 cut(s)	676, 838, 1561, 1901, 2470, 2533
Lsp1109I	GCAGC	7 cut(s)	749, 1285, 1454, 1867, 1916, 1940, 2812
MaeI	CTAG	6 cut(s)	642, 663, 930, 1860, 1934, 2094
MaeII	ACGT	5 cut(s)	570, 1375, 1737, 2005, 2491
MaeIII	GTNAC	4 cut(s)	916, 1371, 2178, 2270
MalI	GATC	9 cut(s)	372, 510, 532, 549, 999, 1658, 2746, 2760, 2868
MbiI	CCGCTC	2 cut(s)	80, 2536
MboI	GATC	9 cut(s)	370, 508, 530, 547, 997, 1656, 2744, 2758, 2866
MhlI	GDGCHC	2 cut(s)	1134, 1558
MlsI	TGGCCA	1 cut(s)	854
MluNI	TGGCCA	1 cut(s)	854
Mly113I	GGCGCC	1 cut(s)	685
MlyI	GAGTC	7 cut(s)	25, 128, 1146, 1405, 1474, 1512, 1933
MmeI	TCCRAC	3 cut(s)	553, 1689, 1923
Mox20I	TGGCCA	1 cut(s)	854
Mph1103I	ATGCAT	1 cut(s)	1573
MroI	TCCGGA	2 cut(s)	2369, 2636
MroXI	GAANNNNTTC	4 cut(s)	580, 603, 948, 1683
MscI	TGGCCA	1 cut(s)	854
MseI	TTAA	7 cut(s)	1382, 1485, 1626, 1674, 1919, 2354, 2576
MslI	CAYNNNNRTG	1 cut(s)	2712
Msp20I	TGGCCA	1 cut(s)	854
MspA1I	CMGCKG	2 cut(s)	1445, 2803
MspCI	CTTAAG	2 cut(s)	1484, 1673
MspI	CCGG	8 cut(s)	143, 200, 1284, 1559, 2370, 2637, 2791, 2857
MspR9I	CCNGG	5 cut(s)	144, 843, 2792, 2858, 2864
Mva1269I	GAATGC	3 cut(s)	1573, 2080, 2436
MvaI	CCWGG	2 cut(s)	843, 2864
MvnI	CGCG	2 cut(s)	22, 2302
MwoI	GCNNNNNNNGC	7 cut(s)	448, 830, 1321, 1442, 1886, 2429, 2718
NarI	GGCGCC	1 cut(s)	685
NciI	CCSGG	3 cut(s)	144, 2792, 2858
NdeI	CATATG	3 cut(s)	849, 1294, 1802
NdeII	GATC	9 cut(s)	370, 508, 530, 547, 997, 1656, 2744, 2758, 2866
NlaIII	CATG	7 cut(s)	446, 718, 1013, 1274, 1525, 1784, 2906
NlaIV	GGNNCC	8 cut(s)	686, 871, 1788, 2200, 2395, 2708, 2855, 2861
NmuCI	GTSAC	2 cut(s)	916, 2270
NsiI	ATGCAT	1 cut(s)	1573
OliI	CACNNNNGTG	1 cut(s)	2712
PagI	TCATGA	1 cut(s)	714
PceI	AGGCCT	1 cut(s)	155
PctI	GAATGC	3 cut(s)	1573, 2080, 2436
PdmI	GAANNNNTTC	4 cut(s)	580, 603, 948, 1683
PfoI	TCCNGGA	1 cut(s)	841
PleI	GAGTC	7 cut(s)	25, 127, 1146, 1404, 1474, 1512, 1932
PluTI	GGCGCC	1 cut(s)	688
PpsI	GAGTC	7 cut(s)	25, 127, 1146, 1404, 1474, 1512, 1932
Ppu21I	YACGTR	2 cut(s)	1376, 1738
PpuMI	RGGWCCY	1 cut(s)	2860
PshAI	GACNNNNGTC	1 cut(s)	2417
Psp5II	RGGWCCY	1 cut(s)	2860
Psp6I	CCWGG	2 cut(s)	841, 2862
PspFI	CCCAGC	1 cut(s)	1445
PspGI	CCWGG	2 cut(s)	841, 2862
PspN4I	GGNNCC	8 cut(s)	686, 871, 1788, 2200, 2395, 2708, 2855, 2861
PspPI	GGNCC	9 cut(s)	632, 890, 908, 1669, 1786, 2212, 2393, 2854, 2860
PspPPI	RGGWCCY	1 cut(s)	2860
PstI	CTGCAG	2 cut(s)	1303, 1958
PvuII	CAGCTG	1 cut(s)	1445
RsaI	GTAC	1 cut(s)	1288
RsaNI	GTAC	1 cut(s)	1287
RseI	CAYNNNNRTG	1 cut(s)	2712
SaqAI	TTAA	7 cut(s)	1382, 1485, 1626, 1674, 1919, 2354, 2576
Sau3AI	GATC	9 cut(s)	370, 508, 530, 547, 997, 1656, 2744, 2758, 2866
Sau96I	GGNCC	9 cut(s)	632, 890, 908, 1669, 1786, 2212, 2393, 2854, 2860
SchI	GAGTC	7 cut(s)	25, 128, 1146, 1405, 1474, 1512, 1933
ScrFI	CCNGG	5 cut(s)	144, 843, 2792, 2858, 2864
SduI	GDGCHC	2 cut(s)	1134, 1558
SfcI	CTRYAG	2 cut(s)	1299, 1954
SfoI	GGCGCC	1 cut(s)	686
SinI	GGWCC	4 cut(s)	890, 1786, 2393, 2860
SmiMI	CAYNNNNRTG	1 cut(s)	2712
SmlI	CTYRAG	4 cut(s)	1484, 1673, 2187, 2528
SmoI	CTYRAG	4 cut(s)	1484, 1673, 2187, 2528
SseBI	AGGCCT	1 cut(s)	155
SspDI	GGCGCC	1 cut(s)	684
SspI	AATATT	1 cut(s)	2125
SspMI	CTAG	6 cut(s)	642, 663, 930, 1860, 1934, 2094
StuI	AGGCCT	1 cut(s)	155
StyD4I	CCNGG	5 cut(s)	142, 841, 2790, 2856, 2862
StyI	CCWWGG	3 cut(s)	662, 2208, 2722
TaaI	ACNGT	6 cut(s)	431, 877, 979, 1255, 2184, 2456
TaiI	ACGT	5 cut(s)	573, 1378, 1740, 2008, 2494
TaqI	TCGA	4 cut(s)	14, 149, 361, 1987
TaqII	GACCGA	2 cut(s)	349, 1755
TauI	GCSGC	3 cut(s)	454, 2112, 2707
Tru1I	TTAA	7 cut(s)	1382, 1485, 1626, 1674, 1919, 2354, 2576
Tru9I	TTAA	7 cut(s)	1382, 1485, 1626, 1674, 1919, 2354, 2576
TscAI	CASTG	7 cut(s)	880, 1558, 1666, 2145, 2461, 2629, 2719
TseFI	GTSAC	2 cut(s)	916, 2270
TseI	GCWGC	7 cut(s)	737, 1298, 1442, 1880, 1904, 1953, 2800
Tsp45I	GTSAC	2 cut(s)	916, 2270
TspGWI	ACGGA	2 cut(s)	24, 2429
TspRI	CASTG	7 cut(s)	880, 1558, 1666, 2145, 2461, 2629, 2719
Vha464I	CTTAAG	2 cut(s)	1484, 1673
VpaK11BI	GGWCC	4 cut(s)	890, 1786, 2393, 2860
XagI	CCTNNNNNAGG	1 cut(s)	2659
XapI	RAATTY	8 cut(s)	463, 503, 601, 937, 1358, 1589, 2066, 2098
XcmI	CCANNNNNNNNNTGG	2 cut(s)	695, 2292
XmaJI	CCTAGG	1 cut(s)	662
XmnI	GAANNNNTTC	4 cut(s)	580, 603, 948, 1683
XspI	CTAG	6 cut(s)	642, 663, 930, 1860, 1934, 2094
Zsp2I	ATGCAT	1 cut(s)	1573

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.