Rorug06G0286800

U2 snRNP-associated SURP motif-containing

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0009276
Physical Location & Seq
Reverse (-)
43687306 .. 43697412
10107 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0286800.1

Sequence Viewer

Length: 4320 bp
ATGGAGGAAGCGCTTGAACTCGCACGCGCCAAGGACACCAAGGAGCGCATGGCCGGAGTGGAGCGCCTCCACCAGCTTCTCGAAGCTTCCAGGAAGAGCCTCACTTCGGCCGAGGTCACTGCGCTCGTCGATTGCTGCATGGATCTTCTCAAGGACAACAACTTCCGAGTCTCCCAGGGCGCTCTTCAGGCCCTCGCCTCCGCCGCCGTCCTCTCCGGCGACCATCTCAAGCTTCATTTCAATGCGCTTGTCCCCGCCGTCGTCGAGCGCCTCGGCGATGCCAAGCAGCCCGTTCGTGACGCCGCCCGGAGGCTCCTGCTTACTCTTATGGAGGTTTCTTCTCCAACACTCATTGTTGAAAGAGCAGGTTCTTATGCCTGGGCACACAAAAGTTGGAGAGTTAGAGAGGAGTTTGCACGAACTGTCACCTCAGCAATTGGACTTTTTGCATCAACCGAACTTCCTCTTCAGCGTGCTATACTTCCTCCGATTCTGCAGATGTTAAGTGATTCAAATCCCGGTGTAAGGGACGCAGCTATATTGTGCATTGAGGAGATGTATACGCAGGCAGGGACTCAGTTCCGTGATGAACTTCAGCGGCACCATCTTCCCATGTCCATGGTGAAGGATATTAATGCAAGGCTAGAGAGGATTGAACCTAAGAATCGCTCATCAGATGGACTTGCTGCTGTAGAAACAAAGCCCTTGAGCCATAACCCAAAGAGAAATAGTCCAAAGGCTAAGAGTTCCTCAAGGGAGGTATCTCTATTTGGAGCGGAAACTGATCCAACTGTAAAATCTGTAGACCCTATAAAAGTTTATTCTGAAAAGGAACTAATAAGAGAAGTGGAGAAAATAGCTTCTACCCTTGTACCAGAGAAAGACTGGTCAATTCGGATTGCTGCAATGCAGAGAATAGAAGGGCTTGTATATGGAGGTGCTGCTGATTATCAATCATTTCGGGGACTTTTGAAGCAACTTGTGAATCCTCTGAACACACAGTTATCTGATCGAAGGTCTAGCATAGTGAAGCAGGCTTGCCATTTGTTATGCTTTTTGTCGAAGGAGCTATTGGGAGATTTTGAGGCATATGCAGAGATTTTCATTCCAGTCCTTTTCAAGCTGGTTGTGATCACTGTACTTGTAATTGCAGAGTCTGCAGATAACTGCATAAAAACGATGTTGCGTAACTGTAAAGTTGCTCGGGTGCTCCCTCGTATTGCGGATTGTGCAAAGAATGACCGTAATGCAATACTTCGAGCAAGGTGCTGTGACTATGCACTTTTGATACTGGAGTATTGGGCCGATGAACCTGAAATACAGAGATCGGCAGATTTGTATGAAGATCTAATAAGATGTTGTGTTGCAGATGCAATGAGCGAGGTCCGTTCAACTGCAAGAATGTGCTATAGAATGTTTTCAAAAACCTGGCCAGAGCGTTCCCGACGACTATTTTCACTATTTGATCCCGTTATTCAAAGGTTAATCAATGAAGAGGATGGGGGGATACATAGGCGACATGCTTCTCCATCTGTTCGTGATAGGGGTACACCGGTCGCATTTACTCCCCAGCCATCTGCTTCTGCTAATCTACCTGGATATGGAACTTCGGCTATTGTTGCAATGGATAGAAGTTCAAGTTTATCTTCTGGGACTTCATTCTCTTCAGGACTTCATCTGTCACAAGCGAAGTCACATGGTAAAGGTACTGAGCGTAGTCTGGAAAGTGTGTTACATGCAAGCAAACAGAAGGTCAGTGCAATTGAAAGCATGCTTAGAGGTTTGGAGTTGTCTGACAGACATAATTCTTCAACTCTCCGGTCATCTAGTCTGGATCTAGGAGTTGACCCTCCATCATCTCGTGATCCACCATTCCCTGCTGCTGTCCCAGCTTCTAATCATCTTTCAAACTCTCTAATGACAGATTCAACCACCTCAAGTAACAAAGGTAGTAACCGCAATGGTGGCCTGGTTTTGTCTGATATCATTACGCAAATTCAAGCTTCCAAAGATTCTGCTAAATCATCATATCGCAGTAATTTATCAGCTGAAACACTGCCAACTGTTTCATCCTACAACATAAAGAGGGCTTCTGAGAGAATCCACGAAAGGGGTTTCATTGAAGAGAATACTGACGGTAGGGAGGCTAGGCGAACTATGAATCATCAAGCTGACAGGCATTATTTGGACACATCTCACAGGGATGGAAACTTTAGGGATTCACATAATAACCATATCCCCAATTTTCAAAGGCCCCTCTTAAGAAAGAATGCTACAGGAAGGATATCTGCAGGCAGGAGGAGAAGTTTTGATGATAGCCAACTCTCACAGGAGATGGCAAATTATGTAGAAGGTCCAGCATCGCTAAATGATGCTCTGAGTGAAGGACTAAGTTCATCTTCAGACTGGTCAGCTAGGGTTGCTGCATTTAATTATCTTCGGTCTTTGTTGCAGCAAGGCCAAAGAGGAATTCAAGAAGTGATTCAGAATTTCGAGAAAGTAATGAAGTTGTTTTTCCAACACTTGGATGATCCGCACCACAAAGTTGCACAAGCAGCCCTTTCAACACTTGCAGACATTATTCCATCATGCCGTAAGCCCTTTGAAAGTTACATGGAAAGGATTCTACCTCATGTTTTCTCACGGTTAATTGACCCAAAGGAGTTAGTTAGGCAACCTTGCTCAACAACTTTGGTCATCGTTGGCAAGACTTACAGTGTAGATTCCCTGTTACCTGCTTTGCTTCGTTCACTAGATGAACAGAGGTCACCAAAGGCTAAATTAGCTGTTATTGAGTTTTCTATTGGTTCATTTAACAAGCATTCAGTGAATACTGAAGGTTCTGGTAATAGTGGCATCCTAAAATTATGGCTTTCGAAGTTGACACCATTGGTCCATGATAAAAACACTAAACTAAAGGAGGCAGCTATTACATGCATAATCTCTGTATACTCCCACTTTGATCCAACAGCTGTCTTGAATTTTATTCTTAGCTTGTCAGTTGAAGAACAAAATTCGTTGAGAAGAGCCTTGAAACAAAGTACTCCTCGCATCGAAGTGGACCTTATGAACTTTTTGCAAAACAAGAAAGAGCGACAGCGCAAGTCTTCTTATGATCCGTCTGATGCAGTTGGAACATCTTCCGAAGAAGGATATGTTAGTGCCTCAAAGAAGAGTCATTTCTTTGGGAGGTATTCTGCTGGTTCAGTTGATAGTGATGGTGGCAGGAAGTGGAGTTCAACCCAAGAAACAGCCTTGGTCACTGGCTCGGTTGGCCAAGCAGCTTCTGATCAGACTGGGGAAAACTTGTATCAGAATTTTGAGACTGGTTCTAACATTGATGTTCTCAATGCAAAATCCAAGGATTCAACTTACATGATCAGTGCCATGAATCAGAGCTCTGGTTCCTGGACTAGCCCTCTGGACAATGGCGAAGGTAGAGTAAATTTAGAAAGTTTGCATACTCATTCCCTGGATGTTAATGGGGTTTTGAACTCGGACCATATTGGGGCTGCTGAGAGCATTGGGCATAGTGAAGCTTCCACCGACTTGGACCAGAATCACCACCAACTTAAAGCTTTAAAGGTTAATTCCATTCCAGACTCAGGTCCCAGCATTCCTCAGATACTCCACCTGATTAGCACTGGAACTGAGGAAAGTCCTGCAGAAAGTAAGCGTGGTGCACTTCAACAGCTAATCGAAGCTTCTAAAACTAATGACCACTCTATTTGGACCAAGTACTTCAATCAAATTTTGACAGTTGTACTTGAGGTGTTGGATGATTTTGACTCCTCCATCCGGGAGCTCTCTCTTTCATTGATTATTGAAATGCTTAAGAACCAGAAAGCTGCAATGGAAGATTCTATTGAGATCGTAATTGAGAAGCTGCTGCATGTTACAAAGGACTTAGTTCCCCAGGTTTCAAATGAATCAGAGCACTGCTTGAGTATTGTTCTGTCGCAGTATGATCCATTCAGATGTTTAAGTGTCATTGTCCCTTTATTGGTAACTGAAGATGAGAAAACTCTTGTTACTTGCATAAACTGTTTGACAAAGCTTGTAGGCAGGCTTTCACAGGAGGAACTAATGGCTCAATTACCATCTTTTTTGCCTGCACTGTTCGAAGCGTTTGGAAATCAGAGTGCTGATGTTCGCAAGACTGTTGTCTTCTGTTTAGTTGATATATATATCATGCTTGGAAAAGCATTCTTGCCATATCTGGAGGGGCTCAACAGCACACAGTTACGGTTGGTGACTATTTATGCTAATCGAATTTCACAGGCGAGGACAGGCACGCCAATAGACACTAACCATGATTAG

Protein Analysis

1439

Amino Acids

159.34

Weight (kDa)

6.74

Isoelectric Point (pI)

46.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CEP104-like_TOG PF21040 10 - 180 7e-10 Centrosomal protein CEP104-like, TOG domain
XMAP215_CLASP_TOG PF21041 11 - 213 6.4e-10 XMAP215/Dis1/CLASP, TOG domain
HEAT_GCN1_C PF25786 18 - 116 2.1e-06 GCN1-like, C-terminal HEAT repeats
CLASP_N PF12348 280 - 496 3.5e-48 CLASP N terminal
CLASP_N PF12348 796 - 987 4.1e-09 CLASP N terminal
CEP104-like_TOG PF21040 800 - 943 1.6e-08 Centrosomal protein CEP104-like, TOG domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 356, 2743
AccB1I GGYRCC 1 cut(s) 600
AccB7I CCANNNNNTGG 1 cut(s) 2524
AccBSI CCGCTC 1 cut(s) 776
AccI GTMKAC 3 cut(s) 560, 804, 2949
AccII CGCG 1 cut(s) 27
AciI CCGC 9 cut(s) 201, 204, 255, 303, 598, 776, 1223, 1957, 2534
AclWI GGATC 9 cut(s) 150, 779, 1460, 1842, 1859, 2525, 2957, 3110, 3962
AcoI YGGCCR 4 cut(s) 51, 108, 1430, 3274
AcsI RAATTY 9 cut(s) 1995, 2469, 2488, 2980, 3013, 3316, 3445, 3750, 4272
AcuI CTGAAG 7 cut(s) 170, 452, 578, 1650, 2385, 2856, 4032
AcyI GRCGYC 1 cut(s) 300
AfaI GTAC 7 cut(s) 873, 1140, 1549, 1708, 3043, 3740, 3765
AfeI AGCGCT 1 cut(s) 12
AflII CTTAAG 2 cut(s) 2260, 3833
AgeI ACCGGT 1 cut(s) 1552
AjnI CCWGG 9 cut(s) 89, 174, 377, 1427, 1594, 1968, 3407, 3471, 3915
AjuI GAANNNNNNNTTGG 4 cut(s) 1055, 1087, 2454, 2486
AloI GAACNNNNNNTCC 2 cut(s) 3220, 3252
Alw21I GWGCWC 5 cut(s) 1212, 3401, 3685, 3807, 3939
Alw26I GTCTC 2 cut(s) 175, 3317
Alw44I GTGCAC 1 cut(s) 3681
AlwI GGATC 9 cut(s) 150, 779, 1460, 1842, 1859, 2525, 2957, 3110, 3962
AlwNI CAGNNNCTG 2 cut(s) 1157, 3287
Ama87I CYCGRG 1 cut(s) 1203
Aor51HI AGCGCT 1 cut(s) 12
AoxI GGCC 9 cut(s) 51, 108, 189, 1302, 1430, 1966, 2252, 2458, 3274
ApaLI GTGCAC 1 cut(s) 3681
ApoI RAATTY 9 cut(s) 1995, 2469, 2488, 2980, 3013, 3316, 3445, 3750, 4272
ArsI GACNNNNNNTTYG 2 cut(s) 1737, 1769
AseI ATTAAT 1 cut(s) 633
AsiGI ACCGGT 1 cut(s) 1552
Asp700I GAANNNNTTC 4 cut(s) 1417, 2481, 2622, 3139
AspLEI GCGC 9 cut(s) 13, 29, 48, 66, 124, 182, 247, 270, 3102
AsuC2I CCSGG 3 cut(s) 307, 519, 3800
AsuHPI GGTGA 5 cut(s) 418, 634, 2760, 3554, 4264
AsuII TTCGAA 2 cut(s) 2876, 4122
AvaI CYCGRG 1 cut(s) 1203
AvaII GGWCC 8 cut(s) 1384, 2354, 2893, 3061, 3499, 3553, 3608, 3732
BaeGI GKGCMC 2 cut(s) 385, 3685
BaeI ACNNNNGTAYC 6 cut(s) 855, 888, 1280, 1313, 1539, 1572
BalI TGGCCA 2 cut(s) 1432, 3276
BanI GGYRCC 1 cut(s) 600
BanII GRGCYC 3 cut(s) 3401, 3807, 4230
BauI CACGAG 1 cut(s) 1860
BbsI GAAGAC 2 cut(s) 3099, 4159
Bbv12I GWGCWC 5 cut(s) 1212, 3401, 3685, 3807, 3939
BbvCI CCTCAGC 1 cut(s) 430
BceAI ACGGC 3 cut(s) 191, 242, 2577
BcgI CGANNNNNNTGC 6 cut(s) 101, 135, 275, 309, 1248, 1282
BciT130I CCWGG 9 cut(s) 91, 176, 379, 1429, 1596, 1970, 3409, 3473, 3917
BciVI GTATCC 1 cut(s) 1500
BclI TGATCA 3 cut(s) 1131, 3289, 3378
BcnI CCSGG 3 cut(s) 307, 519, 3800
BcoDI GTCTC 2 cut(s) 175, 3317
BfaI CTAG 8 cut(s) 644, 1020, 1827, 1838, 2148, 2415, 2753, 3414
BfmI CTRYAG 8 cut(s) 494, 690, 801, 1158, 1408, 2274, 2289, 3663
BfoI RGCGCY 4 cut(s) 14, 67, 183, 271
BfrI CTTAAG 2 cut(s) 2260, 3833
BfuAI ACCTGC 2 cut(s) 356, 2743
BfuI GTATCC 1 cut(s) 1500
BglII AGATCT 1 cut(s) 1345
BmcAI AGTACT 2 cut(s) 3043, 3740
Bme18I GGWCC 8 cut(s) 1384, 2354, 2893, 3061, 3499, 3553, 3608, 3732
BmeT110I CYCGRG 1 cut(s) 1203
BmiI GGNNCC 5 cut(s) 314, 602, 2255, 3406, 3610
BmrI ACTGGG 1 cut(s) 3306
BmsI GCATC 8 cut(s) 268, 458, 1360, 2362, 2369, 2865, 3060, 3115
BmuI ACTGGG 1 cut(s) 3306
BoxI GACNNNNGTC 2 cut(s) 3606, 4163
BpiI GAAGAC 2 cut(s) 3099, 4159
BpmI CTGGAG 2 cut(s) 1313, 4241
Bpu10I CCTNAGC 1 cut(s) 430
Bpu14I TTCGAA 2 cut(s) 2876, 4122
BpuEI CTTGAG 7 cut(s) 134, 212, 727, 736, 1921, 3788, 3964
BpuMI CCSGG 3 cut(s) 307, 519, 3800
BsaBI GATNNNNATC 1 cut(s) 513
BsaHI GRCGYC 1 cut(s) 300
BsaWI WCCGGW 2 cut(s) 1552, 1818
BsaXI ACNNNNNCTCC 2 cut(s) 2290, 2320
Bse118I RCCGGY 1 cut(s) 1552
Bse1I ACTGG 8 cut(s) 890, 1109, 1296, 2411, 3268, 3301, 3331, 3649
Bse3DI GCAATG 5 cut(s) 912, 1380, 1629, 1966, 3858
Bse8I GATNNNNATC 1 cut(s) 513
BseBI CCWGG 9 cut(s) 91, 176, 379, 1429, 1596, 1970, 3409, 3473, 3917
BseGI GGATG 8 cut(s) 1504, 2069, 2209, 2533, 2856, 3481, 3784, 3795
BseJI GATNNNNATC 1 cut(s) 513
BseMI GCAATG 5 cut(s) 912, 1380, 1629, 1966, 3858
BseMII CTCAG 9 cut(s) 444, 590, 1701, 2085, 2369, 3507, 3618, 3635, 3642
BseNI ACTGG 8 cut(s) 890, 1109, 1296, 2411, 3268, 3301, 3331, 3649
BseRI GAGGAG 5 cut(s) 422, 566, 2314, 3036, 3781
BseSI GKGCMC 2 cut(s) 385, 3685
BseX3I CGGCCG 1 cut(s) 108
BseYI CCCAGC 3 cut(s) 1569, 1888, 3611
BsgI GTGCAG 1 cut(s) 4098
Bsh1236I CGCG 1 cut(s) 27
Bsh1285I CGRYCG 2 cut(s) 111, 1557
BshFI GGCC 9 cut(s) 53, 110, 191, 1304, 1432, 1968, 2254, 2460, 3276
BshNI GGYRCC 1 cut(s) 600
BshTI ACCGGT 1 cut(s) 1552
BsiEI CGRYCG 2 cut(s) 111, 1557
BsiHKAI GWGCWC 5 cut(s) 1212, 3401, 3685, 3807, 3939
BsiHKCI CYCGRG 1 cut(s) 1203
BsiSI CCGG 7 cut(s) 54, 216, 307, 519, 1553, 1819, 3799
BslFI GGGAC 8 cut(s) 236, 542, 586, 978, 1666, 1871, 3594, 3980
BsmAI GTCTC 2 cut(s) 175, 3317
BsmFI GGGAC 8 cut(s) 236, 542, 586, 978, 1666, 1871, 3594, 3980
BsmI GAATGC 4 cut(s) 2275, 2821, 3615, 4205
BsnI GGCC 9 cut(s) 53, 110, 191, 1304, 1432, 1968, 2254, 2460, 3276
BsoBI CYCGRG 1 cut(s) 1203
Bsp119I TTCGAA 2 cut(s) 2876, 4122
Bsp1286I GDGCHC 7 cut(s) 385, 1212, 3401, 3685, 3807, 3939, 4230
Bsp19I CCATGG 1 cut(s) 618
BspACI CCGC 9 cut(s) 201, 204, 255, 303, 598, 776, 1223, 1957, 2534
BspANI GGCC 9 cut(s) 53, 110, 191, 1304, 1432, 1968, 2254, 2460, 3276
BspCNI CTCAG 9 cut(s) 443, 589, 1702, 2086, 2370, 3508, 3617, 3634, 3643
BspFNI CGCG 1 cut(s) 27
BspLI GGNNCC 5 cut(s) 314, 602, 2255, 3406, 3610
BspMAI CTGCAG 4 cut(s) 498, 1162, 2293, 3667
BspMI ACCTGC 2 cut(s) 356, 2743
BspPI GGATC 9 cut(s) 150, 779, 1460, 1842, 1859, 2525, 2957, 3110, 3962
BspQI GCTCTTC 3 cut(s) 89, 189, 3019
BspT104I TTCGAA 2 cut(s) 2876, 4122
BspT107I GGYRCC 1 cut(s) 600
BspTI CTTAAG 2 cut(s) 2260, 3833
BsrBI CCGCTC 1 cut(s) 776
BsrDI GCAATG 5 cut(s) 912, 1380, 1629, 1966, 3858
BsrFI RCCGGY 1 cut(s) 1552
BsrI ACTGG 8 cut(s) 890, 1109, 1296, 2411, 3268, 3301, 3331, 3649
BssAI RCCGGY 1 cut(s) 1552
BssNAI GTATAC 2 cut(s) 561, 2950
BssNI GRCGYC 1 cut(s) 300
BssSI CACGAG 1 cut(s) 1860
BssT1I CCWWGG 5 cut(s) 30, 39, 618, 3255, 3360
Bst1107I GTATAC 2 cut(s) 561, 2950
Bst2BI CACGAG 1 cut(s) 1860
Bst2UI CCWGG 9 cut(s) 91, 176, 379, 1429, 1596, 1970, 3409, 3473, 3917
Bst6I CTCTTC 8 cut(s) 89, 189, 471, 1488, 1669, 2118, 3019, 3167
BstACI GRCGYC 1 cut(s) 300
BstAFI CTTAAG 2 cut(s) 2260, 3833
BstAPI GCANNNNNTGC 1 cut(s) 1157
BstBI TTCGAA 2 cut(s) 2876, 4122
BstDSI CCRYGG 1 cut(s) 618
BstEII GGTNACC 1 cut(s) 2766
BstF5I GGATG 8 cut(s) 1504, 2069, 2209, 2533, 2856, 3481, 3784, 3795
BstFNI CGCG 1 cut(s) 27
BstH2I RGCGCY 4 cut(s) 14, 67, 183, 271
BstHHI GCGC 9 cut(s) 13, 29, 48, 66, 124, 182, 247, 270, 3102
BstMAI GTCTC 2 cut(s) 175, 3317
BstMCI CGRYCG 2 cut(s) 111, 1557
BstNI CCWGG 9 cut(s) 91, 176, 379, 1429, 1596, 1970, 3409, 3473, 3917
BstNSI RCATGY 5 cut(s) 1523, 1739, 1774, 2937, 3896
BstPAI GACNNNNGTC 2 cut(s) 3606, 4163
BstPI GGTNACC 1 cut(s) 2766
BstSFI CTRYAG 8 cut(s) 494, 690, 801, 1158, 1408, 2274, 2289, 3663
BstSLI GKGCMC 2 cut(s) 385, 3685
BstUI CGCG 1 cut(s) 27
BstV2I GAAGAC 2 cut(s) 3099, 4159
BstX2I RGATCY 3 cut(s) 142, 1345, 1834
BstXI CCANNNNNNTGG 2 cut(s) 619, 3550
BstYI RGATCY 3 cut(s) 142, 1345, 1834
BstZ17I GTATAC 2 cut(s) 561, 2950
BstZI CGGCCG 1 cut(s) 108
BsuI GTATCC 1 cut(s) 1500
BsuRI GGCC 9 cut(s) 53, 110, 191, 1304, 1432, 1968, 2254, 2460, 3276
BtgI CCRYGG 1 cut(s) 618
BtgZI GCGATG 2 cut(s) 291, 2346
BtsCI GGATG 8 cut(s) 1504, 2069, 2209, 2533, 2856, 3481, 3784, 3795
BtsI GCAGTG 3 cut(s) 117, 2054, 3937
BveI ACCTGC 2 cut(s) 356, 2743
CaiI CAGNNNCTG 2 cut(s) 1157, 3287
CfoI GCGC 9 cut(s) 13, 29, 48, 66, 124, 182, 247, 270, 3102
Cfr10I RCCGGY 1 cut(s) 1552
CseI GACGC 2 cut(s) 308, 539
Csp6I GTAC 7 cut(s) 872, 1139, 1548, 1707, 3042, 3739, 3764
CspAI ACCGGT 1 cut(s) 1552
CspCI CAANNNNNGTGG 2 cut(s) 3710, 3745
CviQI GTAC 7 cut(s) 872, 1139, 1548, 1707, 3042, 3739, 3764
DraI TTTAAA 1 cut(s) 3582
EaeI YGGCCR 4 cut(s) 51, 108, 1430, 3274
EagI CGGCCG 1 cut(s) 108
Eam1104I CTCTTC 8 cut(s) 89, 189, 471, 1488, 1669, 2118, 3019, 3167
EarI CTCTTC 8 cut(s) 89, 189, 471, 1488, 1669, 2118, 3019, 3167
EciI GGCGGA 1 cut(s) 190
Ecl136II GAGCTC 2 cut(s) 3399, 3805
EclXI CGGCCG 1 cut(s) 108
Eco130I CCWWGG 5 cut(s) 30, 39, 618, 3255, 3360
Eco24I GRGCYC 3 cut(s) 3401, 3807, 4230
Eco32I GATATC 2 cut(s) 1984, 2286
Eco47I GGWCC 8 cut(s) 1384, 2354, 2893, 3061, 3499, 3553, 3608, 3732
Eco47III AGCGCT 1 cut(s) 12
Eco52I CGGCCG 1 cut(s) 108
Eco53kI GAGCTC 2 cut(s) 3399, 3805
Eco57I CTGAAG 7 cut(s) 170, 452, 578, 1650, 2385, 2856, 4032
Eco88I CYCGRG 1 cut(s) 1203
Eco91I GGTNACC 1 cut(s) 2766
EcoICRI GAGCTC 2 cut(s) 3399, 3805
EcoO109I RGGNCCY 3 cut(s) 190, 2253, 3608
EcoO65I GGTNACC 1 cut(s) 2766
EcoRI GAATTC 1 cut(s) 2469
EcoRII CCWGG 9 cut(s) 89, 174, 377, 1427, 1594, 1968, 3407, 3471, 3915
EcoRV GATATC 2 cut(s) 1984, 2286
EcoT14I CCWWGG 5 cut(s) 30, 39, 618, 3255, 3360
EcoT22I ATGCAT 1 cut(s) 2939
EcoT38I GRGCYC 3 cut(s) 3401, 3807, 4230
ErhI CCWWGG 5 cut(s) 30, 39, 618, 3255, 3360
FalI AAGNNNNNCTT 8 cut(s) 1630, 1662, 2383, 2415, 2544, 2576, 3048, 3080
FaqI GGGAC 8 cut(s) 236, 542, 586, 978, 1666, 1871, 3594, 3980
FauI CCCGC 1 cut(s) 262
FauNDI CATATG 1 cut(s) 1090
FbaI TGATCA 3 cut(s) 1131, 3289, 3378
FblI GTMKAC 3 cut(s) 560, 804, 2949
FokI GGATG 8 cut(s) 1511, 2056, 2216, 2540, 2843, 3488, 3782, 3791
FriOI GRGCYC 3 cut(s) 3401, 3807, 4230
FspBI CTAG 8 cut(s) 644, 1020, 1827, 1838, 2148, 2415, 2753, 3414
GlaI GCGC 9 cut(s) 12, 28, 47, 65, 123, 181, 246, 269, 3101
GsaI CCCAGC 3 cut(s) 1573, 1892, 3615
GsuI CTGGAG 2 cut(s) 1313, 4241
HaeII RGCGCY 4 cut(s) 14, 67, 183, 271
HaeIII GGCC 9 cut(s) 53, 110, 191, 1304, 1432, 1968, 2254, 2460, 3276
HapII CCGG 7 cut(s) 54, 216, 307, 519, 1553, 1819, 3799
HgaI GACGC 2 cut(s) 308, 539
HhaI GCGC 9 cut(s) 13, 29, 48, 66, 124, 182, 247, 270, 3102
Hin1I GRCGYC 1 cut(s) 300
Hin6I GCGC 9 cut(s) 11, 27, 46, 64, 122, 180, 245, 268, 3100
HinP1I GCGC 9 cut(s) 11, 27, 46, 64, 122, 180, 245, 268, 3100
HincII GTYRAC 2 cut(s) 1846, 2883
HindII GTYRAC 2 cut(s) 1846, 2883
HindIII AAGCTT 7 cut(s) 84, 230, 2001, 3537, 3576, 3702, 4055
HpaII CCGG 7 cut(s) 54, 216, 307, 519, 1553, 1819, 3799
HphI GGTGA 5 cut(s) 418, 634, 2760, 3554, 4264
Hpy166II GTNNAC 9 cut(s) 561, 805, 1550, 1846, 2750, 2883, 2950, 3061, 3683
Hpy8I GTNNAC 9 cut(s) 561, 805, 1550, 1846, 2750, 2883, 2950, 3061, 3683
Hpy99I CGWCG 4 cut(s) 131, 263, 266, 1449
Hsp92I GRCGYC 1 cut(s) 300
HspAI GCGC 9 cut(s) 11, 27, 46, 64, 122, 180, 245, 268, 3100
Ksp22I TGATCA 3 cut(s) 1131, 3289, 3378
LguI GCTCTTC 3 cut(s) 89, 189, 3019
LmnI GCTCC 7 cut(s) 43, 61, 318, 773, 1066, 1215, 3802
LweI GCATC 8 cut(s) 268, 458, 1360, 2362, 2369, 2865, 3060, 3115
MaeI CTAG 8 cut(s) 644, 1020, 1827, 1838, 2148, 2415, 2753, 3414
MbiI CCGCTC 1 cut(s) 776
MfeI CAATTG 2 cut(s) 435, 1761
MflI RGATCY 3 cut(s) 142, 1345, 1834
MhlI GDGCHC 7 cut(s) 385, 1212, 3401, 3685, 3807, 3939, 4230
MlsI TGGCCA 2 cut(s) 1432, 3276
MluNI TGGCCA 2 cut(s) 1432, 3276
MlyI GAGTC 6 cut(s) 177, 568, 1163, 3184, 3596, 3782
MmeI TCCRAC 7 cut(s) 368, 374, 812, 2542, 2990, 3112, 3756
Mox20I TGGCCA 2 cut(s) 1432, 3276
Mph1103I ATGCAT 1 cut(s) 2939
MroXI GAANNNNTTC 4 cut(s) 1417, 2481, 2622, 3139
MscI TGGCCA 2 cut(s) 1432, 3276
MslI CAYNNNNRTG 6 cut(s) 240, 617, 2202, 2526, 3056, 3976
Msp20I TGGCCA 2 cut(s) 1432, 3276
MspA1I CMGCKG 3 cut(s) 598, 2048, 2972
MspCI CTTAAG 2 cut(s) 2260, 3833
MspI CCGG 7 cut(s) 54, 216, 307, 519, 1553, 1819, 3799
MunI CAATTG 2 cut(s) 435, 1761
Mva1269I GAATGC 4 cut(s) 2275, 2821, 3615, 4205
MvaI CCWGG 9 cut(s) 91, 176, 379, 1429, 1596, 1970, 3409, 3473, 3917
MvnI CGCG 1 cut(s) 27
NciI CCSGG 3 cut(s) 307, 519, 3800
NcoI CCATGG 1 cut(s) 618
NdeI CATATG 1 cut(s) 1090
NlaIV GGNNCC 5 cut(s) 314, 602, 2255, 3406, 3610
NmeAIII GCCGAG 2 cut(s) 136, 252
NmuCI GTSAC 9 cut(s) 115, 296, 424, 1271, 1680, 1692, 2766, 3259, 4252
NsiI ATGCAT 1 cut(s) 2939
NspI RCATGY 5 cut(s) 1523, 1739, 1774, 2937, 3896
NspV TTCGAA 2 cut(s) 2876, 4122
PaeI GCATGC 1 cut(s) 1774
PasI CCCWGGG 1 cut(s) 175
PciSI GCTCTTC 3 cut(s) 89, 189, 3019
PctI GAATGC 4 cut(s) 2275, 2821, 3615, 4205
PdmI GAANNNNTTC 4 cut(s) 1417, 2481, 2622, 3139
PflMI CCANNNNNTGG 1 cut(s) 2524
PfoI TCCNGGA 3 cut(s) 89, 3407, 3798
PinAI ACCGGT 1 cut(s) 1552
PleI GAGTC 6 cut(s) 176, 568, 1162, 3183, 3596, 3782
PpsI GAGTC 6 cut(s) 176, 568, 1162, 3183, 3596, 3782
PpuMI RGGWCCY 1 cut(s) 3608
PshAI GACNNNNGTC 2 cut(s) 3606, 4163
PshBI ATTAAT 1 cut(s) 633
Psp124BI GAGCTC 2 cut(s) 3401, 3807
Psp5II RGGWCCY 1 cut(s) 3608
Psp6I CCWGG 9 cut(s) 89, 174, 377, 1427, 1594, 1968, 3407, 3471, 3915
PspEI GGTNACC 1 cut(s) 2766
PspFI CCCAGC 3 cut(s) 1569, 1888, 3611
PspGI CCWGG 9 cut(s) 89, 174, 377, 1427, 1594, 1968, 3407, 3471, 3915
PspN4I GGNNCC 5 cut(s) 314, 602, 2255, 3406, 3610
PspPPI RGGWCCY 1 cut(s) 3608
PsrI GAACNNNNNNTAC 2 cut(s) 1302, 1334
PstI CTGCAG 4 cut(s) 498, 1162, 2293, 3667
PstNI CAGNNNCTG 2 cut(s) 1157, 3287
PsuI RGATCY 3 cut(s) 142, 1345, 1834
PvuII CAGCTG 2 cut(s) 2048, 2972
RsaI GTAC 7 cut(s) 873, 1140, 1549, 1708, 3043, 3740, 3765
RsaNI GTAC 7 cut(s) 872, 1139, 1548, 1707, 3042, 3739, 3764
RseI CAYNNNNRTG 6 cut(s) 240, 617, 2202, 2526, 3056, 3976
SacI GAGCTC 2 cut(s) 3401, 3807
SapI GCTCTTC 3 cut(s) 89, 189, 3019
ScaI AGTACT 2 cut(s) 3043, 3740
SchI GAGTC 6 cut(s) 177, 568, 1163, 3184, 3596, 3782
SduI GDGCHC 7 cut(s) 385, 1212, 3401, 3685, 3807, 3939, 4230
SfaNI GCATC 8 cut(s) 268, 458, 1360, 2362, 2369, 2865, 3060, 3115
SfcI CTRYAG 8 cut(s) 494, 690, 801, 1158, 1408, 2274, 2289, 3663
SfuI TTCGAA 2 cut(s) 2876, 4122
SinI GGWCC 8 cut(s) 1384, 2354, 2893, 3061, 3499, 3553, 3608, 3732
SmiMI CAYNNNNRTG 6 cut(s) 240, 617, 2202, 2526, 3056, 3976
SmlI CTYRAG 9 cut(s) 149, 227, 706, 751, 1936, 2260, 3767, 3833, 3943
SmoI CTYRAG 9 cut(s) 149, 227, 706, 751, 1936, 2260, 3767, 3833, 3943
SphI GCATGC 1 cut(s) 1774
SsiI CCGC 9 cut(s) 201, 204, 255, 303, 598, 776, 1223, 1957, 2534
SspMI CTAG 8 cut(s) 644, 1020, 1827, 1838, 2148, 2415, 2753, 3414
SstI GAGCTC 2 cut(s) 3401, 3807
StyI CCWWGG 5 cut(s) 30, 39, 618, 3255, 3360
TaqII GACCGA 1 cut(s) 2430
TatI WGTACW 4 cut(s) 1138, 3041, 3738, 3763
TauI GCSGC 3 cut(s) 206, 305, 601
TseFI GTSAC 9 cut(s) 115, 296, 424, 1271, 1680, 1692, 2766, 3259, 4252
Tsp45I GTSAC 9 cut(s) 115, 296, 424, 1271, 1680, 1692, 2766, 3259, 4252
TspGWI ACGGA 3 cut(s) 572, 1376, 3108
Van91I CCANNNNNTGG 1 cut(s) 2524
Vha464I CTTAAG 2 cut(s) 2260, 3833
VneI GTGCAC 1 cut(s) 3681
VpaK11BI GGWCC 8 cut(s) 1384, 2354, 2893, 3061, 3499, 3553, 3608, 3732
VspI ATTAAT 1 cut(s) 633
XapI RAATTY 9 cut(s) 1995, 2469, 2488, 2980, 3013, 3316, 3445, 3750, 4272
XceI RCATGY 5 cut(s) 1523, 1739, 1774, 2937, 3896
XcmI CCANNNNNNNNNTGG 2 cut(s) 46, 882
XmiI GTMKAC 3 cut(s) 560, 804, 2949
XmnI GAANNNNTTC 4 cut(s) 1417, 2481, 2622, 3139
XspI CTAG 8 cut(s) 644, 1020, 1827, 1838, 2148, 2415, 2753, 3414
ZrmI AGTACT 2 cut(s) 3043, 3740
Zsp2I ATGCAT 1 cut(s) 2939
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.