Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr08

Orthogroup ID

OG0006486

Physical Location & Seq

Genomic Coordinates Reverse (-)

4701285 .. 4705253

Gene Structure 3969 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD08G1059000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1878 bp

ATGACCGTTGAGCGGGTACTCGACGAACCCAGAGACCAGTTTCCCATTGGGATGCGTGTGCTTGCCGTGGACGATGATCCCATTTGCCTCAAGCTCTTAGAGGCTTTGCTCCGTCGATGCCAATATCATGTTACGACGACGAGTCAGGCAATCACTGCATTGGAGTTGTTGAGAGAGAACAAGGATAAGTTTGATCTGGTTATCAGTGACGTGCATATGCCAGACATGGATGGATTCAAGCTGCTTGAGCTTGTCGGGCTTGAGATGGATCTCCCTGTTATAATGTTGTCTGTAAATGGGGATCACAAGCTTGTGATGAAGGGAATCACCCATGGAGCCTGCGATTACCTCCTAAAACCCGTTCGAATTGAGCAGCTGAAGAACATCTGGCAACACGTGATAAGGAGAAAGAAGGTTGATTCAAAGGACCAGAACTCTGGCAATCAAGACAAGTCTGCTGGATGTGGCGAAGGAGGAGTGGGGTCAGTAGGCACAGGAAATTCAGATCAGAATGCAAAGCTCAATAAGAAACGTAAGGATCAGAATGATGGCGAGGATGAGGATCATGATGAGGATGAATATGAAAATGACGATCCTTCAACCCAGAAAAAACCTCGTGTTGTTTGGTCTGTGGATCTGCATCGCAAGTTTGTGGCCGCTGTGAATCAGTTGGGCATTGACAAGGCTGTACCGAAAAAGATTCTAGATTTGATGAATGTTGAAAAGCTTACAAGAGAAAATGTTGCAAGCCATCTCCAGAAATATAGGCTCTACCTGAAAAGAATCAGCTGTGTGGCAAACCAACAAGCTAATATGGTGGCAGCCTTAGGCACTTCGGATTCTTCATATTTGCGAACGGCTTCTATGAATGGAGTTGGAAATTACCATACATTGACTGGACCTGCGCAGTTTCATAACAATGGATACAGATCCTTCCCGCCTAGTGGGATGATTGGTAGATTGAACACTCCTGCTGGAGTGGGTCTGCATTGCCTTCCTTCATCCGGAATGTTTCAATTGGGTCATGCACAGAATCCAAGCAACGCCATTAATGATCAGCCAGTCATGTTTCCTGGAAACCGTAATGGAAATATGCTTCCGCCACACTTGGAACTTGATCAACAACATAATAGGGGTTCTACCTATGGCGCACCAATTTATCCCGATTCCAGTGGTTTTACAGATACAAAAGTGACGAATAGGTCAAATGATCCTTTTGGTCTTACAGACAACACAATGTTAAGACATACTCGGGATTCCGAAGTTGGAAGAAATCATGTAATGCAGTCTTCCGTATCAATGCCGTCCTTAAAACCAGTACTGGCAGCCCCATTTCTGGATCACGGACGGTTGAATGACAACTGGCCAACCGCTGCTCAGCCATCTGCGTTCCGGTCAAATTCTTTCTCAGCGGTCGACTCTTTTAAACAACCTACTCTCCTTGATTCAAGAATAGGATCACAGATCCCGGCATCTACAATGAGCAATAATTCGGATCCAACAATTAACAATGACCTGTTTCTGGGGTGGGATGAGCCAAAGCAGGATTCCCCGTTCCAGTCAGATGTCATGTGTAGCTCAATGAACAATTTGATGCCTGTCAATGATAATGTGGCTCCTTCCCACCAGAATTTGGACGCCAGAAGTTCAGCCTTCCAAAGAAACAGCGAGTTCAATTTGATCGGACAACCAAATTTTCTTGACCCTTTACTCATGAACTATGATGGTGTTGAAATATCAACTATGGATGCACCATTGAAGTTGAAGCAGCAGGGGTACCTTATGGACCAACGAAAGCCACAGGGGGTTTATGTCCCCCACAATGCTGGTTCGTTGGATGAGTTAGTGAGCTCAATGATCAAACAGGAAAGGAGATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000156 GO:0000160 GO:0001101 GO:0003674 GO:0003700 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0006950 GO:0007154 GO:0007165 GO:0007275 GO:0008150 GO:0009414 GO:0009415 GO:0009628 GO:0009653 GO:0009719 GO:0009725 GO:0009735 GO:0009736 GO:0009755 GO:0009888 GO:0009889 GO:0009966 GO:0009987 GO:0010015 GO:0010033 GO:0010035 GO:0010073 GO:0010074 GO:0010075 GO:0010082 GO:0010380 GO:0010468 GO:0010492 GO:0010556 GO:0010646 GO:0019219 GO:0019222 GO:0019827 GO:0022622 GO:0023051 GO:0023052 GO:0031323 GO:0031326 GO:0031537 GO:0032501 GO:0032502 GO:0032870 GO:0035556 GO:0040008 GO:0042221 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0048364 GO:0048367 GO:0048507 GO:0048509 GO:0048580 GO:0048583 GO:0048638 GO:0048731 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051171 GO:0051193 GO:0051239 GO:0051252 GO:0051716 GO:0060089 GO:0060255 GO:0065007 GO:0070887 GO:0071310 GO:0071368 GO:0071495 GO:0080022 GO:0080036 GO:0080050 GO:0080090 GO:0080113 GO:0090056 GO:0098727 GO:0099402 GO:0140110 GO:1901401 GO:1901463 GO:1901700 GO:1903506 GO:1905392 GO:2000026 GO:2000112 GO:2000241 GO:2000280 GO:2001141

KEGG Pathways

Metabolic & Signaling

ko04075 map04075

Pfam Domains

Protein Families

Myb_DNA-binding Response_reg

Protein Analysis

626

Amino Acids

69.43

Weight (kDa)

6.03

Isoelectric Point (pI)

40.8

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Response_reg	PF00072	20 - 128	7.7e-24	Response regulator receiver domain
Myb_DNA-binding	PF00249	206 - 256	3.2e-07	Myb-like DNA-binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0006486)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G25180 AT4G31920
fragaria_vesca	FvH4_2g40890 FvH4_2g40890 FvH4_2g40890 FvH4_2g40890 FvH4_2g40890 FvH4_2g40890
malus_domestica	MD08G1059000.v1.1 MD15G1037400.v1.1
prunus_persica	Prupe.1G391400_v2.0.a1 Prupe.1G391400_v2.0.a1
pyrus_communis	pycom08g04890 pycom15g03450 pycom15g03460
rosa_chinensis	RchiOBHm_Chr6g0299681
rosa_laevigata	RLG00000011397
rosa_multiflora	Rmu_co8516769.1_g000001 Rmu_sc0002895.1_g000008
rosa_roxburghii	Rroxscaffold_7G00168450
rosa_rugosa	Rorug06G0291500
rosa_samantha	Rh6AG402300 Rh6BG410800 Rh6CG416600 Rh6DG403700
rosa_wichuraiana	Rw6G035220

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	281
AasI	GACNNNNNNGTC	1 cut(s)	1201
Acc16I	TGCGCA	1 cut(s)	906
Acc36I	ACCTGC	1 cut(s)	910
Acc65I	GGTACC	1 cut(s)	1776
AccB1I	GGYRCC	1 cut(s)	1776
AccB7I	CCANNNNNTGG	1 cut(s)	1633
AccBSI	CCGCTC	1 cut(s)	13
AccI	GTMKAC	1 cut(s)	1416
AccIII	TCCGGA	1 cut(s)	1004
AciI	CCGC	6 cut(s)	13, 657, 938, 1100, 1371, 1412
AcoI	YGGCCR	2 cut(s)	654, 1364
AcsI	RAATTY	4 cut(s)	499, 1399, 1630, 1693
AcuI	CTGAAG	1 cut(s)	398
AcvI	CACGTG	1 cut(s)	397
AcyI	GRCGYC	1 cut(s)	1638
AfaI	GTAC	4 cut(s)	18, 690, 1320, 1778
AfiI	CCNNNNNNNGG	6 cut(s)	12, 944, 1004, 1336, 1522, 1633
AflIII	ACRYGT	1 cut(s)	394
AhdI	GACNNNNNGTC	1 cut(s)	141
AjiI	CACGTC	1 cut(s)	211
AjnI	CCWGG	1 cut(s)	1072
AjuI	GAANNNNNNNTTGG	2 cut(s)	1531, 1563
AloI	GAACNNNNNNTCC	2 cut(s)	1538, 1570
Alw21I	GWGCWC	1 cut(s)	1853
Alw26I	GTCTC	1 cut(s)	27
Ama87I	CYCGRG	1 cut(s)	1251
Aor13HI	TCCGGA	1 cut(s)	1004
AoxI	GGCC	2 cut(s)	654, 1364
ApeKI	GCWGC	6 cut(s)	241, 373, 821, 1325, 1373, 1768
ApoI	RAATTY	4 cut(s)	499, 1399, 1630, 1693
AseI	ATTAAT	1 cut(s)	1050
Asp700I	GAANNNNTTC	1 cut(s)	859
Asp718I	GGTACC	1 cut(s)	1776
AspLEI	GCGC	2 cut(s)	907, 1151
AspS9I	GGNCC	3 cut(s)	427, 899, 1786
AsuC2I	CCSGG	1 cut(s)	1469
AsuHPI	GGTGA	1 cut(s)	319
AsuII	TTCGAA	1 cut(s)	364
AvaI	CYCGRG	1 cut(s)	1251
AvaII	GGWCC	3 cut(s)	427, 899, 1786
AxyI	CCTNAGG	1 cut(s)	826
BaeI	ACNNNNGTAYC	2 cut(s)	1176, 1209
BalI	TGGCCA	1 cut(s)	1366
BamHI	GGATCC	1 cut(s)	1495
BanI	GGYRCC	1 cut(s)	1776
BanII	GRGCYC	1 cut(s)	1853
BauI	CACGAG	1 cut(s)	615
BbrPI	CACGTG	1 cut(s)	397
BbsI	GAAGAC	1 cut(s)	1281
Bbv12I	GWGCWC	1 cut(s)	1853
BbvI	GCAGC	6 cut(s)	228, 385, 833, 1337, 1360, 1780
BccI	CCATC	6 cut(s)	224, 259, 542, 759, 1390, 1718
BceAI	ACGGC	3 cut(s)	50, 873, 1288
BciT130I	CCWGG	1 cut(s)	1074
BciVI	GTATCC	1 cut(s)	917
BclI	TGATCA	3 cut(s)	1054, 1117, 1857
BcnI	CCSGG	1 cut(s)	1469
BcoDI	GTCTC	1 cut(s)	27
BfaI	CTAG	2 cut(s)	704, 942
BfuAI	ACCTGC	1 cut(s)	910
BfuI	GTATCC	1 cut(s)	917
BisI	GCNGC	7 cut(s)	242, 374, 657, 822, 1326, 1374, 1769
BlpI	GCTNAGC	1 cut(s)	1377
BlsI	GCNGC	7 cut(s)	243, 375, 658, 823, 1327, 1375, 1770
BmcAI	AGTACT	1 cut(s)	1320
Bme1390I	CCNGG	2 cut(s)	1074, 1469
Bme18I	GGWCC	3 cut(s)	427, 899, 1786
BmeRI	GACNNNNNGTC	1 cut(s)	141
BmeT110I	CYCGRG	1 cut(s)	1251
BmgBI	CACGTC	1 cut(s)	211
BmgT120I	GGNCC	3 cut(s)	427, 899, 1786
BmiI	GGNNCC	4 cut(s)	337, 1497, 1617, 1778
BmrFI	CCNGG	2 cut(s)	1074, 1469
BmsI	GCATC	6 cut(s)	42, 107, 649, 1481, 1584, 1738
BpiI	GAAGAC	1 cut(s)	1281
BpmI	CTGGAG	2 cut(s)	740, 996
Bpu1102I	GCTNAGC	1 cut(s)	1377
Bpu14I	TTCGAA	1 cut(s)	364
BpuEI	CTTGAG	3 cut(s)	74, 266, 281
BpuMI	CCSGG	1 cut(s)	1469
BsaAI	YACGTR	1 cut(s)	397
BsaBI	GATNNNNATC	3 cut(s)	561, 639, 928
BsaHI	GRCGYC	1 cut(s)	1638
BsaI	GGTCTC	1 cut(s)	27
BsaJI	CCNNGG	2 cut(s)	66, 331
BsaWI	WCCGGW	2 cut(s)	1004, 1392
BsaXI	ACNNNNNCTCC	2 cut(s)	1422, 1452
Bsc4I	CCNNNNNNNGG	6 cut(s)	12, 944, 1004, 1336, 1522, 1633
Bse1I	ACTGG	8 cut(s)	37, 901, 1061, 1170, 1316, 1326, 1367, 1558
Bse21I	CCTNAGG	1 cut(s)	826
Bse3DI	GCAATG	1 cut(s)	988
Bse8I	GATNNNNATC	3 cut(s)	561, 639, 928
BseAI	TCCGGA	1 cut(s)	1004
BseBI	CCWGG	1 cut(s)	1074
BseDI	CCNNGG	2 cut(s)	66, 331
BseJI	GATNNNNATC	3 cut(s)	561, 639, 928
BseLI	CCNNNNNNNGG	6 cut(s)	12, 944, 1004, 1336, 1522, 1633
BseMI	GCAATG	1 cut(s)	988
BseMII	CTCAG	2 cut(s)	1391, 1422
BseNI	ACTGG	8 cut(s)	37, 901, 1061, 1170, 1316, 1326, 1367, 1558
BseRI	GAGGAG	1 cut(s)	489
BseXI	GCAGC	6 cut(s)	228, 385, 833, 1337, 1360, 1780
Bsh1285I	CGRYCG	1 cut(s)	1416
BshFI	GGCC	2 cut(s)	656, 1366
BshNI	GGYRCC	1 cut(s)	1776
BsiEI	CGRYCG	1 cut(s)	1416
BsiHKAI	GWGCWC	1 cut(s)	1853
BsiHKCI	CYCGRG	1 cut(s)	1251
BsiSI	CCGG	3 cut(s)	1005, 1393, 1469
BslFI	GGGAC	1 cut(s)	1799
BslI	CCNNNNNNNGG	6 cut(s)	12, 944, 1004, 1336, 1522, 1633
BsmAI	GTCTC	1 cut(s)	27
BsmFI	GGGAC	1 cut(s)	1799
BsmI	GAATGC	1 cut(s)	517
BsnI	GGCC	2 cut(s)	656, 1366
Bso31I	GGTCTC	1 cut(s)	27
BsoBI	CYCGRG	1 cut(s)	1251
Bsp119I	TTCGAA	1 cut(s)	364
Bsp1286I	GDGCHC	1 cut(s)	1853
Bsp13I	TCCGGA	1 cut(s)	1004
Bsp1720I	GCTNAGC	1 cut(s)	1377
Bsp19I	CCATGG	1 cut(s)	331
BspACI	CCGC	6 cut(s)	13, 657, 938, 1100, 1371, 1412
BspANI	GGCC	2 cut(s)	656, 1366
BspCNI	CTCAG	2 cut(s)	1390, 1421
BspEI	TCCGGA	1 cut(s)	1004
BspHI	TCATGA	2 cut(s)	565, 1713
BspLI	GGNNCC	4 cut(s)	337, 1497, 1617, 1778
BspMI	ACCTGC	1 cut(s)	910
BspT104I	TTCGAA	1 cut(s)	364
BspT107I	GGYRCC	1 cut(s)	1776
BspTNI	GGTCTC	1 cut(s)	27
BsrBI	CCGCTC	1 cut(s)	13
BsrDI	GCAATG	1 cut(s)	988
BsrI	ACTGG	8 cut(s)	37, 901, 1061, 1170, 1316, 1326, 1367, 1558
BssECI	CCNNGG	2 cut(s)	66, 331
BssNI	GRCGYC	1 cut(s)	1638
BssSI	CACGAG	1 cut(s)	615
BssT1I	CCWWGG	1 cut(s)	331
Bst2BI	CACGAG	1 cut(s)	615
Bst2UI	CCWGG	1 cut(s)	1074
Bst4CI	ACNGT	3 cut(s)	7, 1082, 1350
BstACI	GRCGYC	1 cut(s)	1638
BstAPI	GCANNNNNTGC	1 cut(s)	155
BstBAI	YACGTR	1 cut(s)	397
BstBI	TTCGAA	1 cut(s)	364
BstC8I	GCNNGC	3 cut(s)	63, 340, 748
BstDEI	CTNAG	4 cut(s)	97, 826, 1377, 1408
BstDSI	CCRYGG	2 cut(s)	66, 331
BstHHI	GCGC	2 cut(s)	907, 1151
BstMAI	GTCTC	1 cut(s)	27
BstMCI	CGRYCG	1 cut(s)	1416
BstMWI	GCNNNNNNNGC	3 cut(s)	155, 247, 256
BstNI	CCWGG	1 cut(s)	1074
BstSCI	CCNGG	2 cut(s)	1072, 1467
BstV1I	GCAGC	6 cut(s)	228, 385, 833, 1337, 1360, 1780
BstV2I	GAAGAC	1 cut(s)	1281
BstX2I	RGATCY	5 cut(s)	268, 634, 929, 1464, 1495
BstXI	CCANNNNNNTGG	2 cut(s)	437, 1826
BstYI	RGATCY	5 cut(s)	268, 634, 929, 1464, 1495
Bsu36I	CCTNAGG	1 cut(s)	826
BsuI	GTATCC	1 cut(s)	917
BsuRI	GGCC	2 cut(s)	656, 1366
BtgI	CCRYGG	2 cut(s)	66, 331
BtgZI	GCGATG	1 cut(s)	626
BtrI	CACGTC	1 cut(s)	211
BtsI	GCAGTG	1 cut(s)	153
BtsIMutI	CAGTG	3 cut(s)	153, 211, 1177
BveI	ACCTGC	1 cut(s)	910
Cac8I	GCNNGC	3 cut(s)	63, 340, 748
CciI	TCATGA	2 cut(s)	565, 1713
CfoI	GCGC	2 cut(s)	907, 1151
Cfr13I	GGNCC	3 cut(s)	427, 899, 1786
CseI	GACGC	1 cut(s)	1646
Csp6I	GTAC	4 cut(s)	17, 689, 1319, 1777
CviAII	CATG	9 cut(s)	128, 226, 332, 566, 1025, 1066, 1277, 1570, 1714
CviQI	GTAC	4 cut(s)	17, 689, 1319, 1777
DdeI	CTNAG	4 cut(s)	97, 826, 1377, 1408
DraI	TTTAAA	1 cut(s)	1426
DrdI	GACNNNNNNGTC	1 cut(s)	1201
DriI	GACNNNNNGTC	1 cut(s)	141
DseDI	GACNNNNNNGTC	1 cut(s)	1201
EaeI	YGGCCR	2 cut(s)	654, 1364
Eam1105I	GACNNNNNGTC	1 cut(s)	141
EciI	GGCGGA	1 cut(s)	1089
Ecl136II	GAGCTC	1 cut(s)	1851
Eco130I	CCWWGG	1 cut(s)	331
Eco24I	GRGCYC	1 cut(s)	1853
Eco31I	GGTCTC	1 cut(s)	27
Eco47I	GGWCC	3 cut(s)	427, 899, 1786
Eco53kI	GAGCTC	1 cut(s)	1851
Eco57I	CTGAAG	1 cut(s)	398
Eco72I	CACGTG	1 cut(s)	397
Eco81I	CCTNAGG	1 cut(s)	826
Eco88I	CYCGRG	1 cut(s)	1251
EcoICRI	GAGCTC	1 cut(s)	1851
EcoRII	CCWGG	1 cut(s)	1072
EcoT14I	CCWWGG	1 cut(s)	331
EcoT38I	GRGCYC	1 cut(s)	1853
ErhI	CCWWGG	1 cut(s)	331
FaeI	CATG	9 cut(s)	131, 229, 335, 569, 1028, 1069, 1280, 1573, 1717
FaqI	GGGAC	1 cut(s)	1799
FatI	CATG	9 cut(s)	127, 225, 331, 565, 1024, 1065, 1276, 1569, 1713
FauI	CCCGC	2 cut(s)	6, 945
FauNDI	CATATG	1 cut(s)	216
FbaI	TGATCA	3 cut(s)	1054, 1117, 1857
FblI	GTMKAC	1 cut(s)	1416
Fnu4HI	GCNGC	7 cut(s)	242, 374, 657, 822, 1326, 1374, 1769
FriOI	GRGCYC	1 cut(s)	1853
Fsp4HI	GCNGC	7 cut(s)	242, 374, 657, 822, 1326, 1374, 1769
FspBI	CTAG	2 cut(s)	704, 942
FspI	TGCGCA	1 cut(s)	906
GlaI	GCGC	2 cut(s)	906, 1150
GluI	GCNGC	7 cut(s)	242, 374, 657, 822, 1326, 1374, 1769
GsuI	CTGGAG	2 cut(s)	740, 996
HaeIII	GGCC	2 cut(s)	656, 1366
HapII	CCGG	3 cut(s)	1005, 1393, 1469
HgaI	GACGC	1 cut(s)	1646
HhaI	GCGC	2 cut(s)	907, 1151
Hin1I	GRCGYC	1 cut(s)	1638
Hin1II	CATG	9 cut(s)	131, 229, 335, 569, 1028, 1069, 1280, 1573, 1717
Hin6I	GCGC	2 cut(s)	905, 1149
HinP1I	GCGC	2 cut(s)	905, 1149
HincII	GTYRAC	1 cut(s)	1417
HindII	GTYRAC	1 cut(s)	1417
HindIII	AAGCTT	2 cut(s)	308, 725
HpaII	CCGG	3 cut(s)	1005, 1393, 1469
HphI	GGTGA	1 cut(s)	319
Hpy166II	GTNNAC	2 cut(s)	70, 1417
Hpy188I	TCNGA	8 cut(s)	505, 510, 543, 838, 1261, 1495, 1564, 1685
Hpy8I	GTNNAC	2 cut(s)	70, 1417
Hpy99I	CGWCG	4 cut(s)	26, 117, 139, 142
HpyAV	CCTTC	9 cut(s)	313, 406, 464, 606, 943, 1004, 1008, 1629, 1663
HpyCH4III	ACNGT	3 cut(s)	7, 1082, 1350
HpyCH4IV	ACGT	3 cut(s)	210, 396, 532
HpyCH4V	TGCA	9 cut(s)	158, 214, 515, 640, 746, 988, 1028, 1285, 1751
HpyF10VI	GCNNNNNNNGC	3 cut(s)	155, 247, 256
HpyF3I	CTNAG	4 cut(s)	97, 826, 1377, 1408
HpySE526I	ACGT	3 cut(s)	210, 396, 532
Hsp92I	GRCGYC	1 cut(s)	1638
Hsp92II	CATG	9 cut(s)	131, 229, 335, 569, 1028, 1069, 1280, 1573, 1717
HspAI	GCGC	2 cut(s)	905, 1149
Kpn2I	TCCGGA	1 cut(s)	1004
KpnI	GGTACC	1 cut(s)	1780
Ksp22I	TGATCA	3 cut(s)	1054, 1117, 1857
LmnI	GCTCC	3 cut(s)	114, 335, 1621
Lsp1109I	GCAGC	6 cut(s)	228, 385, 833, 1337, 1360, 1780
LweI	GCATC	6 cut(s)	42, 107, 649, 1481, 1584, 1738
MaeI	CTAG	2 cut(s)	704, 942
MaeII	ACGT	3 cut(s)	210, 396, 532
MaeIII	GTNAC	3 cut(s)	130, 206, 1192
MbiI	CCGCTC	1 cut(s)	13
MboII	GAAGA	4 cut(s)	391, 834, 1281, 1281
MfeI	CAATTG	1 cut(s)	1016
MflI	RGATCY	5 cut(s)	268, 634, 929, 1464, 1495
MhlI	GDGCHC	1 cut(s)	1853
MlsI	TGGCCA	1 cut(s)	1366
MluNI	TGGCCA	1 cut(s)	1366
MlyI	GAGTC	2 cut(s)	151, 1412
MmeI	TCCRAC	4 cut(s)	856, 1246, 1523, 1815
MnlI	CCTC	8 cut(s)	94, 98, 359, 467, 547, 553, 565, 624
Mox20I	TGGCCA	1 cut(s)	1366
MroI	TCCGGA	1 cut(s)	1004
MroXI	GAANNNNTTC	1 cut(s)	859
MscI	TGGCCA	1 cut(s)	1366
MseI	TTAA	5 cut(s)	1050, 1241, 1310, 1425, 1506
MslI	CAYNNNNRTG	2 cut(s)	50, 918
Msp20I	TGGCCA	1 cut(s)	1366
MspA1I	CMGCKG	5 cut(s)	376, 659, 789, 1373, 1412
MspI	CCGG	3 cut(s)	1005, 1393, 1469
MspR9I	CCNGG	2 cut(s)	1074, 1469
MunI	CAATTG	1 cut(s)	1016
Mva1269I	GAATGC	1 cut(s)	517
MvaI	CCWGG	1 cut(s)	1074
MwoI	GCNNNNNNNGC	3 cut(s)	155, 247, 256
NciI	CCSGG	1 cut(s)	1469
NcoI	CCATGG	1 cut(s)	331
NdeI	CATATG	1 cut(s)	216
NlaIII	CATG	9 cut(s)	131, 229, 335, 569, 1028, 1069, 1280, 1573, 1717
NlaIV	GGNNCC	4 cut(s)	337, 1497, 1617, 1778
NmuCI	GTSAC	2 cut(s)	206, 1192
NsbI	TGCGCA	1 cut(s)	906
NspV	TTCGAA	1 cut(s)	364
PagI	TCATGA	2 cut(s)	565, 1713
PctI	GAATGC	1 cut(s)	517
PdmI	GAANNNNTTC	1 cut(s)	859
PflMI	CCANNNNNTGG	1 cut(s)	1633
PfoI	TCCNGGA	1 cut(s)	1072
PkrI	GCNGC	7 cut(s)	243, 375, 658, 823, 1327, 1375, 1770
PleI	GAGTC	2 cut(s)	150, 1412
PmaCI	CACGTG	1 cut(s)	397
PmlI	CACGTG	1 cut(s)	397
PpsI	GAGTC	2 cut(s)	150, 1412
Ppu21I	YACGTR	1 cut(s)	397
PshBI	ATTAAT	1 cut(s)	1050
PsiI	TTATAA	1 cut(s)	281
Psp124BI	GAGCTC	1 cut(s)	1853
Psp6I	CCWGG	1 cut(s)	1072
PspCI	CACGTG	1 cut(s)	397
PspGI	CCWGG	1 cut(s)	1072
PspN4I	GGNNCC	4 cut(s)	337, 1497, 1617, 1778
PspPI	GGNCC	3 cut(s)	427, 899, 1786
PsuI	RGATCY	5 cut(s)	268, 634, 929, 1464, 1495
PvuII	CAGCTG	2 cut(s)	376, 789
RsaI	GTAC	4 cut(s)	18, 690, 1320, 1778
RsaNI	GTAC	4 cut(s)	17, 689, 1319, 1777
RseI	CAYNNNNRTG	2 cut(s)	50, 918
SacI	GAGCTC	1 cut(s)	1853
SalI	GTCGAC	1 cut(s)	1415
SaqAI	TTAA	5 cut(s)	1050, 1241, 1310, 1425, 1506
SatI	GCNGC	7 cut(s)	242, 374, 657, 822, 1326, 1374, 1769
Sau96I	GGNCC	3 cut(s)	427, 899, 1786
ScaI	AGTACT	1 cut(s)	1320
SchI	GAGTC	2 cut(s)	151, 1412
ScrFI	CCNGG	2 cut(s)	1074, 1469
SduI	GDGCHC	1 cut(s)	1853
SfaNI	GCATC	6 cut(s)	42, 107, 649, 1481, 1584, 1738
SfuI	TTCGAA	1 cut(s)	364
SinI	GGWCC	3 cut(s)	427, 899, 1786
SmiMI	CAYNNNNRTG	2 cut(s)	50, 918
SmlI	CTYRAG	3 cut(s)	89, 245, 260
SmoI	CTYRAG	3 cut(s)	89, 245, 260
SsiI	CCGC	6 cut(s)	13, 657, 938, 1100, 1371, 1412
SspMI	CTAG	2 cut(s)	704, 942
SstI	GAGCTC	1 cut(s)	1853
StyD4I	CCNGG	2 cut(s)	1072, 1467
StyI	CCWWGG	1 cut(s)	331
TaaI	ACNGT	3 cut(s)	7, 1082, 1350
TaiI	ACGT	3 cut(s)	213, 399, 535
TaqI	TCGA	4 cut(s)	21, 115, 364, 1416
TatI	WGTACW	1 cut(s)	1318
TauI	GCSGC	1 cut(s)	659
Tru1I	TTAA	5 cut(s)	1050, 1241, 1310, 1425, 1506
Tru9I	TTAA	5 cut(s)	1050, 1241, 1310, 1425, 1506
TscAI	CASTG	3 cut(s)	160, 211, 1177
TseFI	GTSAC	2 cut(s)	206, 1192
TseI	GCWGC	6 cut(s)	241, 373, 821, 1325, 1373, 1768
Tsp45I	GTSAC	2 cut(s)	206, 1192
TspGWI	ACGGA	3 cut(s)	101, 1282, 1359
TspRI	CASTG	3 cut(s)	160, 211, 1177
Van91I	CCANNNNNTGG	1 cut(s)	1633
VpaK11BI	GGWCC	3 cut(s)	427, 899, 1786
VspI	ATTAAT	1 cut(s)	1050
XapI	RAATTY	4 cut(s)	499, 1399, 1630, 1693
XbaI	TCTAGA	1 cut(s)	703
XcmI	CCANNNNNNNNNTGG	2 cut(s)	44, 893
XmiI	GTMKAC	1 cut(s)	1416
XmnI	GAANNNNTTC	1 cut(s)	859
XspI	CTAG	2 cut(s)	704, 942
ZrmI	AGTACT	1 cut(s)	1320

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.