MD08G1078700.v1.1
ERF Family

Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0005362
Physical Location & Seq
Reverse (-)
6555333 .. 6560489
5157 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1078700.v1.1.491

Sequence Viewer

Length: 2544 bp
ATGGAGAAGATCTGCGTCGCGGTTCGGGTGAGGCCTCCGGTGACTCACGATTCCTCCAGCGGAGCCTTCTGGAAGGTCGACGACAACCGCATTTCGCTCCACAAGTCCCACGGCACGCCGATCTCCGGCATCTCCTACGCTTTCGACCATGTGTTCGATGAAGGTTGTACGAATTCTAGGGTTTACGAGCTTCTCACTAAGGACATTATTCATGCCGCAGTTGAAGGATTCAATGGAACTGCGTTTGCTTATGGGCAGACCAGCAGTGGGAAGACTTTCACCATGAATGGTTCCGAAACAGATCCGGGTATCATTCATCGAGCTGTTAGAGATGTCTTTGACAGAATCCAGATGATGTCCCATCGGGAGTTTCTGATTCGAGTATCCTACATGGAATTGTACAATGAGGAAATTAACGACCTTTTTGAAGTAGAGAACCAGAAATTGCAGATTCATGAGAGTTTGGAGCGTGGCATATTTGTTGCTGGCCTCAGGGAGGAAATTGTCAGTAACGCTGAACAGGTGTTGAAGCTCATTGAATCCGGAGAAGTTAATAGGCACTTTGGAGAAACAAATATGAATGCTCGGAGTAGTAGATCTCACACAATATTCAGAATGGTAATTGAAAGCCATTCGAAGGACATTACTTGTTCAAGTACTGATGCTATTCGTGTCTCAGTCTTGAATTTGGTAGATTTAGCTGGTTCTGAACGGATTGCCAAAACTGGAGCTGGCGGAGTACGTCTGAAGGAGGGAAAGTACATAAACAAGAGCTTAATGATTCTTGGAAATGTAATTAACAAACTTAGTGATGGCGCAAAAAACAGGGGTCACATTCCTTATCGCGATAGTAAGCTTACCCGTATACTTCAACCTGCCCTTGGAGGCAATGCAAAAACTTCAATTATTTGTACTATAGCACCAGAAGAGGTACACATTGAGGAAACAAAGGGGACCCTTCAATTTGCTAGCAGAGCTAAGCGCATCACTAATTGTGCTCAAGTTAATGAGATTTTGACAGATGCAGCCTTACTGAAGCGACAAAAACAAGAGATAGAAGAGCTTCGTATGAAACTTCAGGGATCCCATGCTGGGGTGCTGGAGCAAGAAATACTGAAGTTGCGAAACGACTTGCTTCAGTATGAACTAGAACGCGAGAAGCTTGAAATGGAACTGGAAGAGCAGCGGAAATCACATAAACAACGCGAACAATGCATCAGGGATCAACAGATGAAAACTGACAATCTCAGTAGTCTTTCTACTATTTCAGACTTTGAGAGAAGTTCTAGTCAGGCACAAGATTCTGGGAGACAAAGCCTTAAGGAAGTTAGTAGTGACAGCAGTAGTAAATCTCAAGGAGATATCTTCAGTACCCCATCCAATTTCAAGGCAGCTCCCCATTCCTTCGTTGTCAAGCGATCAAATTATTCACAGTTGCCCAGCTTTAGCCCTCTTCCAGATGCTTTTAGCAATGTGGTTGATGAAGACACATGGTTTAAAATGAACAAAGGTTTCATAGCTGACCTTGATTCCCTTCAGATAACTCCTGCTAGAAAAGTTCAATCCTTTCCGCCAAGTGATGAAACTCCCGGCTCAAATGAGAACTACAAGCACGAGGTCCACAATTTGAAGAGACAGCTAGAAATTGCTGTTGAAGAGAGAGATGATCTCAAGAGGAAACATGCCGAACAATCTGTATTGAATGATCAATTAGTTCGGGAAATATCTGAACTCCAAAAAGAAGCACAACTAATTCGAGACATCCCTCAAAGGCTTTGTGAATGTGCAGCAACCTGCAAAGATATTTATGTGGATGTTTTATCAAAGACTCAGAGCTTTATATCTGATGAAAAATCTTCTGCGGCAACATTGCTTTCGAGCATAAGTGAAATTGGCACAAGCCTCTTTACGACTCTCGAAGCCCAATTCTCAGCGGCATTTGATGAGGACCAAAGATTGTTCTCTAGGAATAGTTCTCTAATTGAAGAACAGCGAAAAATTCTCTCCGAGAGGTTGAACAGCACAATCAAATTGTTCGTATCATCAGAACCATCAAATCTTGAGAATGAGCAAGTGAGAAATTCGCTATGCCGCAGTGAATACAAGGACTGCACAAGGGGAGGAGAAACTGCCTGTTGGGAGGGAAAACTAAGCAATGAGCTCAGCACTATCAAGGCAAGATACAATGGCCTGGAGGAAGAGCTAGATTCGAATAACCAGCTTCTAGAGGAATCCAAGCAAAGATATGATGCCATGGAAGCTGAGTTTCGGCTTTTGAAAGAAGAAAGAGATTCCTTGCACAAAAAGGTATCTGAATCATCTCAAACACTTGCTCTGGTTACTGACCAAAAGGAAAATGTTGTGAAGGATCTGAATCACGAGTTACTGAGAAGGAAAGATCTTGAAGAAAAAATCAAACATTTCAGTGTCGCTTTTGGGTGTCGGAAGACATCGCTCATGTCTTTCCACAGCGAGTTTAAGTCTAAGATTGAGAGTTTGAGAGCCAAAAATCCAGTTTCAGTACCCAAATCTGTTGGATGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000070 GO:0000226 GO:0000278 GO:0000280 GO:0000775 GO:0000776 GO:0000779 GO:0000793 GO:0000819 GO:0001932 GO:0001934 GO:0002376 GO:0002478 GO:0002495 GO:0002504 GO:0003674 GO:0003774 GO:0003777 GO:0003824 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0005737 GO:0005819 GO:0005828 GO:0005829 GO:0005856 GO:0005871 GO:0005874 GO:0005875 GO:0005876 GO:0006810 GO:0006890 GO:0006928 GO:0006996 GO:0007010 GO:0007017 GO:0007018 GO:0007049 GO:0007051 GO:0007052 GO:0007059 GO:0007079 GO:0007080 GO:0007088 GO:0007346 GO:0008017 GO:0008092 GO:0008150 GO:0008608 GO:0009893 GO:0009987 GO:0010562 GO:0010564 GO:0010604 GO:0010965 GO:0015630 GO:0015631 GO:0016043 GO:0016192 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019220 GO:0019222 GO:0019882 GO:0019884 GO:0019886 GO:0022402 GO:0022607 GO:0030071 GO:0030496 GO:0031323 GO:0031325 GO:0031399 GO:0031401 GO:0031974 GO:0031981 GO:0032268 GO:0032270 GO:0032991 GO:0033043 GO:0033044 GO:0033045 GO:0033047 GO:0033674 GO:0034508 GO:0034622 GO:0042325 GO:0042327 GO:0043085 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043515 GO:0043549 GO:0043933 GO:0044085 GO:0044093 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044430 GO:0044444 GO:0044446 GO:0044464 GO:0044877 GO:0045859 GO:0045860 GO:0045937 GO:0048002 GO:0048193 GO:0048285 GO:0048518 GO:0048522 GO:0050000 GO:0050789 GO:0050790 GO:0050794 GO:0051128 GO:0051171 GO:0051173 GO:0051174 GO:0051179 GO:0051233 GO:0051234 GO:0051246 GO:0051247 GO:0051276 GO:0051303 GO:0051305 GO:0051310 GO:0051315 GO:0051338 GO:0051347 GO:0051382 GO:0051383 GO:0051640 GO:0051641 GO:0051649 GO:0051656 GO:0051726 GO:0051783 GO:0051983 GO:0060255 GO:0065003 GO:0065004 GO:0065007 GO:0065009 GO:0070013 GO:0070925 GO:0071824 GO:0071840 GO:0072686 GO:0080090 GO:0098687 GO:0098813 GO:0099080 GO:0099081 GO:0099512 GO:0099513 GO:0099606 GO:0099607 GO:0140014 GO:1901987 GO:1901990 GO:1902099 GO:1902850 GO:1903047 GO:1905818 GO:1990023
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Kinesin

Protein Analysis

848

Amino Acids

95.76

Weight (kDa)

6.0

Isoelectric Point (pI)

51.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Kinesin PF00225 9 - 329 1.4e-109 Kinesin motor domain
Microtub_bd PF16796 37 - 141 2.2e-18 Microtubule binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 883, 1802
AccI GTMKAC 2 cut(s) 78, 865
AccII CGCG 4 cut(s) 20, 846, 1155, 1206
AccIII TCCGGA 1 cut(s) 542
AclWI GGATC 5 cut(s) 296, 1077, 1090, 1230, 2376
AcsI RAATTY 4 cut(s) 172, 685, 1998, 2080
AcuI CTGAAG 7 cut(s) 767, 1055, 1061, 1121, 1136, 1351, 1520
AfaI GTAC 9 cut(s) 169, 401, 658, 741, 761, 913, 933, 1372, 2523
AfiI CCNNNNNNNGG 7 cut(s) 125, 267, 496, 637, 881, 1092, 1093
AflII CTTAAG 1 cut(s) 1319
AjnI CCWGG 1 cut(s) 2190
Alw21I GWGCWC 2 cut(s) 1000, 2163
Alw26I GTCTC 4 cut(s) 679, 1303, 1627, 1752
AlwI GGATC 5 cut(s) 296, 1077, 1090, 1230, 2376
Aor13HI TCCGGA 1 cut(s) 542
AoxI GGCC 3 cut(s) 32, 487, 2188
ApeKI GCWGC 4 cut(s) 1025, 1183, 1391, 1787
ApoI RAATTY 4 cut(s) 172, 685, 1998, 2080
ArsI GACNNNNNNTTYG 2 cut(s) 137, 169
Asp700I GAANNNNTTC 2 cut(s) 275, 1062
AspLEI GCGC 2 cut(s) 818, 984
AspS9I GGNCC 3 cut(s) 954, 1618, 1948
AsuC2I CCSGG 2 cut(s) 306, 1590
AsuHPI GGTGA 3 cut(s) 40, 52, 271
AsuII TTCGAA 2 cut(s) 635, 2210
AsuNHI GCTAGC 1 cut(s) 968
AvaII GGWCC 3 cut(s) 954, 1618, 1948
AxyI CCTNAGG 1 cut(s) 491
BamHI GGATCC 1 cut(s) 1082
BanII GRGCYC 1 cut(s) 2163
BauI CACGAG 2 cut(s) 1613, 2378
BbsI GAAGAC 3 cut(s) 278, 1491, 2453
Bbv12I GWGCWC 2 cut(s) 1000, 2163
BbvI GCAGC 4 cut(s) 1037, 1195, 1403, 1799
BccI CCATC 4 cut(s) 369, 806, 1384, 2059
BceAI ACGGC 1 cut(s) 127
BciT130I CCWGG 1 cut(s) 2192
BciVI GTATCC 1 cut(s) 394
BclI TGATCA 1 cut(s) 1705
BcnI CCSGG 2 cut(s) 306, 1590
BcoDI GTCTC 4 cut(s) 679, 1303, 1627, 1752
BfaI CTAG 9 cut(s) 177, 969, 1148, 1287, 1551, 1640, 1965, 2204, 2225
BfmI CTRYAG 1 cut(s) 915
BfrI CTTAAG 1 cut(s) 1319
BfuAI ACCTGC 2 cut(s) 883, 1802
BfuI GTATCC 1 cut(s) 394
BglII AGATCT 3 cut(s) 9, 596, 2398
BisI GCNGC 8 cut(s) 216, 1026, 1184, 1392, 1788, 1863, 1935, 2092
BlpI GCTNAGC 2 cut(s) 978, 2162
BlsI GCNGC 8 cut(s) 217, 1027, 1185, 1393, 1789, 1864, 1936, 2093
BmcAI AGTACT 1 cut(s) 658
Bme1390I CCNGG 3 cut(s) 306, 1590, 2192
Bme18I GGWCC 3 cut(s) 954, 1618, 1948
BmgT120I GGNCC 3 cut(s) 954, 1618, 1948
BmiI GGNNCC 5 cut(s) 64, 292, 955, 956, 1084
BmrFI CCNGG 3 cut(s) 306, 1590, 2192
BmsI GCATC 7 cut(s) 138, 652, 993, 1012, 1224, 1450, 2239
BmtI GCTAGC 1 cut(s) 972
BpiI GAAGAC 3 cut(s) 278, 1491, 2453
BplI GAGNNNNNCTC 2 cut(s) 1653, 1685
BpmI CTGGAG 4 cut(s) 40, 747, 1121, 2213
Bpu1102I GCTNAGC 2 cut(s) 978, 2162
Bpu14I TTCGAA 2 cut(s) 635, 2210
BpuEI CTTGAG 4 cut(s) 984, 1338, 1655, 2081
BpuMI CCSGG 2 cut(s) 306, 1590
BsaBI GATNNNNATC 1 cut(s) 2373
BsaJI CCNNGG 3 cut(s) 109, 880, 2253
BsaWI WCCGGW 2 cut(s) 37, 542
BsaXI ACNNNNNCTCC 4 cut(s) 38, 68, 743, 773
Bsc4I CCNNNNNNNGG 7 cut(s) 125, 267, 496, 637, 881, 1092, 1093
Bse1I ACTGG 3 cut(s) 730, 1179, 2513
Bse21I CCTNAGG 1 cut(s) 491
Bse3DI GCAATG 4 cut(s) 895, 1477, 1868, 2161
Bse8I GATNNNNATC 1 cut(s) 2373
BseAI TCCGGA 1 cut(s) 542
BseBI CCWGG 1 cut(s) 2192
BseDI CCNNGG 3 cut(s) 109, 880, 2253
BseGI GGATG 4 cut(s) 1376, 1761, 1819, 2543
BseJI GATNNNNATC 1 cut(s) 2373
BseLI CCNNNNNNNGG 7 cut(s) 125, 267, 496, 637, 881, 1092, 1093
BseMI GCAATG 4 cut(s) 895, 1477, 1868, 2161
BseMII CTCAG 8 cut(s) 505, 690, 1261, 1844, 1944, 2176, 2253, 2378
BseNI ACTGG 3 cut(s) 730, 1179, 2513
BseRI GAGGAG 1 cut(s) 2136
BseXI GCAGC 4 cut(s) 1037, 1195, 1403, 1799
BseYI CCCAGC 2 cut(s) 1091, 1439
BsgI GTGCAG 2 cut(s) 1806, 2095
Bsh1236I CGCG 4 cut(s) 20, 846, 1155, 1206
BshFI GGCC 3 cut(s) 34, 489, 2190
BsiHKAI GWGCWC 2 cut(s) 1000, 2163
BsiSI CCGG 5 cut(s) 38, 126, 305, 543, 1590
BslFI GGGAC 3 cut(s) 91, 343, 967
BslI CCNNNNNNNGG 7 cut(s) 125, 267, 496, 637, 881, 1092, 1093
BsmAI GTCTC 4 cut(s) 679, 1303, 1627, 1752
BsmFI GGGAC 3 cut(s) 91, 343, 967
BsmI GAATGC 1 cut(s) 586
BsnI GGCC 3 cut(s) 34, 489, 2190
Bsp119I TTCGAA 2 cut(s) 635, 2210
Bsp1286I GDGCHC 2 cut(s) 1000, 2163
Bsp13I TCCGGA 1 cut(s) 542
Bsp1407I TGTACA 1 cut(s) 399
Bsp1720I GCTNAGC 2 cut(s) 978, 2162
Bsp19I CCATGG 1 cut(s) 2253
Bsp68I TCGCGA 1 cut(s) 846
BspANI GGCC 3 cut(s) 34, 489, 2190
BspCNI CTCAG 8 cut(s) 504, 689, 1260, 1843, 1943, 2175, 2254, 2379
BspEI TCCGGA 1 cut(s) 542
BspFNI CGCG 4 cut(s) 20, 846, 1155, 1206
BspHI TCATGA 1 cut(s) 454
BspLI GGNNCC 5 cut(s) 64, 292, 955, 956, 1084
BspMI ACCTGC 2 cut(s) 883, 1802
BspOI GCTAGC 1 cut(s) 972
BspPI GGATC 5 cut(s) 296, 1077, 1090, 1230, 2376
BspQI GCTCTTC 3 cut(s) 1053, 1173, 2193
BspT104I TTCGAA 2 cut(s) 635, 2210
BspTI CTTAAG 1 cut(s) 1319
BsrDI GCAATG 4 cut(s) 895, 1477, 1868, 2161
BsrGI TGTACA 1 cut(s) 399
BsrI ACTGG 3 cut(s) 730, 1179, 2513
BssECI CCNNGG 3 cut(s) 109, 880, 2253
BssNAI GTATAC 1 cut(s) 866
BssSI CACGAG 2 cut(s) 1613, 2378
BssT1I CCWWGG 2 cut(s) 880, 2253
Bst1107I GTATAC 1 cut(s) 866
Bst2BI CACGAG 2 cut(s) 1613, 2378
Bst2UI CCWGG 1 cut(s) 2192
Bst4CI ACNGT 1 cut(s) 1434
Bst6I CTCTTC 7 cut(s) 921, 1053, 1173, 1458, 1625, 1650, 2193
BstAFI CTTAAG 1 cut(s) 1319
BstAUI TGTACA 1 cut(s) 399
BstBI TTCGAA 2 cut(s) 635, 2210
BstC8I GCNNGC 4 cut(s) 116, 487, 733, 970
BstDSI CCRYGG 2 cut(s) 109, 2253
BstENI CCTNNNNNAGG 1 cut(s) 494
BstF5I GGATG 4 cut(s) 1376, 1761, 1819, 2543
BstFNI CGCG 4 cut(s) 20, 846, 1155, 1206
BstHHI GCGC 2 cut(s) 818, 984
BstMAI GTCTC 4 cut(s) 679, 1303, 1627, 1752
BstMWI GCNNNNNNNGC 3 cut(s) 974, 1212, 2258
BstNI CCWGG 1 cut(s) 2192
BstNSI RCATGY 1 cut(s) 1685
BstSCI CCNGG 3 cut(s) 304, 1588, 2190
BstSFI CTRYAG 1 cut(s) 915
BstUI CGCG 4 cut(s) 20, 846, 1155, 1206
BstV1I GCAGC 4 cut(s) 1037, 1195, 1403, 1799
BstV2I GAAGAC 3 cut(s) 278, 1491, 2453
BstX2I RGATCY 6 cut(s) 9, 301, 596, 1082, 2368, 2398
BstYI RGATCY 6 cut(s) 9, 301, 596, 1082, 2368, 2398
BstZ17I GTATAC 1 cut(s) 866
Bsu36I CCTNAGG 1 cut(s) 491
BsuI GTATCC 1 cut(s) 394
BsuRI GGCC 3 cut(s) 34, 489, 2190
BtgI CCRYGG 2 cut(s) 109, 2253
BtgZI GCGATG 1 cut(s) 2436
BtsCI GGATG 4 cut(s) 1376, 1761, 1819, 2543
BtsI GCAGTG 2 cut(s) 271, 2101
BtsIMutI CAGTG 3 cut(s) 271, 2101, 2431
BtuMI TCGCGA 1 cut(s) 846
BveI ACCTGC 2 cut(s) 883, 1802
Cac8I GCNNGC 4 cut(s) 116, 487, 733, 970
CciI TCATGA 1 cut(s) 454
CfoI GCGC 2 cut(s) 818, 984
Cfr13I GGNCC 3 cut(s) 954, 1618, 1948
CseI GACGC 1 cut(s) 4
Csp6I GTAC 9 cut(s) 168, 400, 657, 740, 760, 912, 932, 1371, 2522
CviQI GTAC 9 cut(s) 168, 400, 657, 740, 760, 912, 932, 1371, 2522
DraI TTTAAA 1 cut(s) 1498
Eam1104I CTCTTC 7 cut(s) 921, 1053, 1173, 1458, 1625, 1650, 2193
EarI CTCTTC 7 cut(s) 921, 1053, 1173, 1458, 1625, 1650, 2193
EciI GGCGGA 2 cut(s) 750, 1560
Ecl136II GAGCTC 1 cut(s) 2161
Eco130I CCWWGG 2 cut(s) 880, 2253
Eco147I AGGCCT 1 cut(s) 34
Eco24I GRGCYC 1 cut(s) 2163
Eco32I GATATC 1 cut(s) 1363
Eco47I GGWCC 3 cut(s) 954, 1618, 1948
Eco53kI GAGCTC 1 cut(s) 2161
Eco57I CTGAAG 7 cut(s) 767, 1055, 1061, 1121, 1136, 1351, 1520
Eco81I CCTNAGG 1 cut(s) 491
EcoICRI GAGCTC 1 cut(s) 2161
EcoNI CCTNNNNNAGG 1 cut(s) 494
EcoO109I RGGNCCY 1 cut(s) 954
EcoRI GAATTC 1 cut(s) 172
EcoRII CCWGG 1 cut(s) 2190
EcoRV GATATC 1 cut(s) 1363
EcoT14I CCWWGG 2 cut(s) 880, 2253
EcoT22I ATGCAT 1 cut(s) 1217
EcoT38I GRGCYC 1 cut(s) 2163
ErhI CCWWGG 2 cut(s) 880, 2253
FaqI GGGAC 3 cut(s) 91, 343, 967
FbaI TGATCA 1 cut(s) 1705
FblI GTMKAC 2 cut(s) 78, 865
Fnu4HI GCNGC 8 cut(s) 216, 1026, 1184, 1392, 1788, 1863, 1935, 2092
FokI GGATG 3 cut(s) 1363, 1748, 1826
FriOI GRGCYC 1 cut(s) 2163
Fsp4HI GCNGC 8 cut(s) 216, 1026, 1184, 1392, 1788, 1863, 1935, 2092
FspBI CTAG 9 cut(s) 177, 969, 1148, 1287, 1551, 1640, 1965, 2204, 2225
GlaI GCGC 2 cut(s) 817, 983
GluI GCNGC 8 cut(s) 216, 1026, 1184, 1392, 1788, 1863, 1935, 2092
GsaI CCCAGC 2 cut(s) 1095, 1443
GsuI CTGGAG 4 cut(s) 40, 747, 1121, 2213
HaeIII GGCC 3 cut(s) 34, 489, 2190
HapII CCGG 5 cut(s) 38, 126, 305, 543, 1590
HgaI GACGC 1 cut(s) 4
HhaI GCGC 2 cut(s) 818, 984
Hin6I GCGC 2 cut(s) 816, 982
HinP1I GCGC 2 cut(s) 816, 982
HincII GTYRAC 1 cut(s) 79
HindII GTYRAC 1 cut(s) 79
HindIII AAGCTT 2 cut(s) 854, 1160
HpaII CCGG 5 cut(s) 38, 126, 305, 543, 1590
HphI GGTGA 3 cut(s) 40, 52, 271
Hpy166II GTNNAC 5 cut(s) 79, 184, 866, 934, 1621
Hpy8I GTNNAC 5 cut(s) 79, 184, 866, 934, 1621
Hpy99I CGWCG 2 cut(s) 20, 83
HpyCH4III ACNGT 1 cut(s) 1434
HpyCH4IV ACGT 1 cut(s) 742
HpyCH4V TGCA 8 cut(s) 448, 893, 1025, 1215, 1787, 1797, 2112, 2299
HpyF10VI GCNNNNNNNGC 3 cut(s) 974, 1212, 2258
HpySE526I ACGT 1 cut(s) 742
HspAI GCGC 2 cut(s) 816, 982
KflI GGGWCCC 1 cut(s) 954
Kpn2I TCCGGA 1 cut(s) 542
Ksp22I TGATCA 1 cut(s) 1705
LguI GCTCTTC 3 cut(s) 1053, 1173, 2193
LmnI GCTCC 6 cut(s) 62, 102, 466, 728, 1102, 1399
Lsp1109I GCAGC 4 cut(s) 1037, 1195, 1403, 1799
LweI GCATC 7 cut(s) 138, 652, 993, 1012, 1224, 1450, 2239
MaeI CTAG 9 cut(s) 177, 969, 1148, 1287, 1551, 1640, 1965, 2204, 2225
MaeII ACGT 1 cut(s) 742
MaeIII GTNAC 6 cut(s) 40, 509, 830, 1334, 2338, 2382
MflI RGATCY 6 cut(s) 9, 301, 596, 1082, 2368, 2398
MhlI GDGCHC 2 cut(s) 1000, 2163
MlyI GAGTC 3 cut(s) 37, 1822, 1906
MmeI TCCRAC 2 cut(s) 2423, 2515
Mph1103I ATGCAT 1 cut(s) 1217
MroI TCCGGA 1 cut(s) 542
MroXI GAANNNNTTC 2 cut(s) 275, 1062
MseI TTAA 8 cut(s) 414, 552, 776, 798, 1005, 1320, 1497, 2478
MslI CAYNNNNRTG 1 cut(s) 2424
MspA1I CMGCKG 3 cut(s) 60, 1186, 1934
MspCI CTTAAG 1 cut(s) 1319
MspI CCGG 5 cut(s) 38, 126, 305, 543, 1590
MspR9I CCNGG 3 cut(s) 306, 1590, 2192
Mva1269I GAATGC 1 cut(s) 586
MvaI CCWGG 1 cut(s) 2192
MvnI CGCG 4 cut(s) 20, 846, 1155, 1206
MwoI GCNNNNNNNGC 3 cut(s) 974, 1212, 2258
NciI CCSGG 2 cut(s) 306, 1590
NcoI CCATGG 1 cut(s) 2253
NheI GCTAGC 1 cut(s) 968
NlaIV GGNNCC 5 cut(s) 64, 292, 955, 956, 1084
NmuCI GTSAC 3 cut(s) 40, 830, 1334
NruI TCGCGA 1 cut(s) 846
NsiI ATGCAT 1 cut(s) 1217
NspI RCATGY 1 cut(s) 1685
NspV TTCGAA 2 cut(s) 635, 2210
PagI TCATGA 1 cut(s) 454
PceI AGGCCT 1 cut(s) 34
PciSI GCTCTTC 3 cut(s) 1053, 1173, 2193
PctI GAATGC 1 cut(s) 586
PdmI GAANNNNTTC 2 cut(s) 275, 1062
PkrI GCNGC 8 cut(s) 217, 1027, 1185, 1393, 1789, 1864, 1936, 2093
PleI GAGTC 3 cut(s) 37, 1822, 1906
PpsI GAGTC 3 cut(s) 37, 1822, 1906
PpuMI RGGWCCY 1 cut(s) 954
Psp124BI GAGCTC 1 cut(s) 2163
Psp5II RGGWCCY 1 cut(s) 954
Psp6I CCWGG 1 cut(s) 2190
PspFI CCCAGC 2 cut(s) 1091, 1439
PspGI CCWGG 1 cut(s) 2190
PspN4I GGNNCC 5 cut(s) 64, 292, 955, 956, 1084
PspPI GGNCC 3 cut(s) 954, 1618, 1948
PspPPI RGGWCCY 1 cut(s) 954
PsuI RGATCY 6 cut(s) 9, 301, 596, 1082, 2368, 2398
RruI TCGCGA 1 cut(s) 846
RsaI GTAC 9 cut(s) 169, 401, 658, 741, 761, 913, 933, 1372, 2523
RsaNI GTAC 9 cut(s) 168, 400, 657, 740, 760, 912, 932, 1371, 2522
RseI CAYNNNNRTG 1 cut(s) 2424
SacI GAGCTC 1 cut(s) 2163
SalI GTCGAC 1 cut(s) 77
SapI GCTCTTC 3 cut(s) 1053, 1173, 2193
SaqAI TTAA 8 cut(s) 414, 552, 776, 798, 1005, 1320, 1497, 2478
SatI GCNGC 8 cut(s) 216, 1026, 1184, 1392, 1788, 1863, 1935, 2092
Sau96I GGNCC 3 cut(s) 954, 1618, 1948
ScaI AGTACT 1 cut(s) 658
SchI GAGTC 3 cut(s) 37, 1822, 1906
ScrFI CCNGG 3 cut(s) 306, 1590, 2192
SduI GDGCHC 2 cut(s) 1000, 2163
SfaNI GCATC 7 cut(s) 138, 652, 993, 1012, 1224, 1450, 2239
SfcI CTRYAG 1 cut(s) 915
SfuI TTCGAA 2 cut(s) 635, 2210
SinI GGWCC 3 cut(s) 954, 1618, 1948
SmiMI CAYNNNNRTG 1 cut(s) 2424
SmlI CTYRAG 5 cut(s) 999, 1319, 1353, 1670, 2060
SmoI CTYRAG 5 cut(s) 999, 1319, 1353, 1670, 2060
SseBI AGGCCT 1 cut(s) 34
SspI AATATT 1 cut(s) 609
SspMI CTAG 9 cut(s) 177, 969, 1148, 1287, 1551, 1640, 1965, 2204, 2225
SstI GAGCTC 1 cut(s) 2163
StuI AGGCCT 1 cut(s) 34
StyD4I CCNGG 3 cut(s) 304, 1588, 2190
StyI CCWWGG 2 cut(s) 880, 2253
TaaI ACNGT 1 cut(s) 1434
TaiI ACGT 1 cut(s) 745
TatI WGTACW 4 cut(s) 399, 656, 759, 911
TauI GCSGC 4 cut(s) 218, 1865, 1937, 2094
Tru1I TTAA 8 cut(s) 414, 552, 776, 798, 1005, 1320, 1497, 2478
Tru9I TTAA 8 cut(s) 414, 552, 776, 798, 1005, 1320, 1497, 2478
TscAI CASTG 3 cut(s) 271, 2101, 2431
TseFI GTSAC 3 cut(s) 40, 830, 1334
TseI GCWGC 4 cut(s) 1025, 1183, 1391, 1787
Tsp45I GTSAC 3 cut(s) 40, 830, 1334
TspGWI ACGGA 1 cut(s) 727
TspRI CASTG 3 cut(s) 271, 2101, 2431
Vha464I CTTAAG 1 cut(s) 1319
VpaK11BI GGWCC 3 cut(s) 954, 1618, 1948
XagI CCTNNNNNAGG 1 cut(s) 494
XapI RAATTY 4 cut(s) 172, 685, 1998, 2080
XbaI TCTAGA 1 cut(s) 2224
XceI RCATGY 1 cut(s) 1685
XmiI GTMKAC 2 cut(s) 78, 865
XmnI GAANNNNTTC 2 cut(s) 275, 1062
XspI CTAG 9 cut(s) 177, 969, 1148, 1287, 1551, 1640, 1965, 2204, 2225
ZrmI AGTACT 1 cut(s) 658
Zsp2I ATGCAT 1 cut(s) 1217
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.