MD15G1065700.v1.1
ERF Family

Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0005362
Physical Location & Seq
Reverse (-)
4567451 .. 4572496
5046 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1065700.v1.1.491

Sequence Viewer

Length: 2682 bp
ATGAATAAATGGTATTTTCGTAAACTGAACGAAATTCAAATCCTGCTCCCCTCCAAAAAGCAAAGAGCATTTGGCCGCCTGCGGAAGTTGAACTCCATCAGTCCCTCAAACTCTCGGAGAGAAACAACATCGACGGCTCTAATGGAGAAGATCTGCGTTGCGGTTCGAGTGAGGCCTCCGGTAGCTCACGATTCCTCCACCGGAACTATCTGGAAGGTCGACGACAACCGCATTTCGCTCCACAAGCCCCACGGCACGCCGATCTCCGGTATCTCCTACGCTTTCGATCATGTGTTCGATGAAGATTGTACGAATTCTAGGGTTTATGAGCTGCTCACCAAGGACATTATTCATGCCGTAGTCGAAGGATTTAATGGAACTGCGTTTGCTTATGGACAGACTAGCAGTGGGAAGACTTTCACCATGAATGGTTCCAAAACAGATCCAGGTATCATTCATCGAGCCGTTAGAGATGTTTTTGACAGAATCCAGATGATGTCACACCGGGAGTTTCTGATTCGAGTATCCTACATGGAATTGTACAATGAGGAAATTAACGACCTTTTTGCAGTAGAGAATCAGAAATTGCAGATTCATGAGAGTTTGGAGCGTGGCATATTTGTTGCTGGCCTCAGGGAGGAAATTGTCAGTAATGCTGAACAGGTGTTGAAGCTCATTGAATCTGGAGAAGTTAATAGGCACTTTGGAGAAACAAACATGAATGCTCGGAGTAGTAGATCTCACACAATATTCAGAATGGTAATTGAAAGCCATGCGAAGGACACTAGTTCAAGTGCTGATGCTATTCGTGTCTCTGTCTTGAATTTGGTAGATTTAGCTGGTTCTGAAAGAATTGCTAAAACTGGAGCTGGCGGAGTACGTCTGAAGGAAGGAAAGTACATAAACAAGAGCTTAATGATTCTTGGTAATGTAATTAACAAACTTAGTGATGGCGCAAAAAACAGGGGTCACATCCCTTATCGTGATAGTAAGCTTACCCGTATACTTCAACCTGCCCTTGGAGGCAATGCCAAAACTTCAATTATATGCACTATAGCACCAGAAGAGGTACACATTGAGGAAACAAAGGGAACCCTTCAATTTGCTAGCAGAGCTAAGTGCATCACTAATTGTGCTCAAGTGAATGAGATTTTGACAGACGCAGCCTTACTGAAGCGGCAAAAACAAGAGATAGAAGATCTTCGTATGAAACTTCAGGGATCACATGCCGGGGTGCTGGAGCAAGAAATATTGAAGTTAAGAAATGACTTACTACAGTATGAACTAGAGCGTGAGAAGCTGGAAATGGAACTGGAAGAGCAGCGGAAATCACATAAACAACAGGAACAATGCATCAGGGATCAACAGATGAAAACTGACAATCTCAGTGGTCTTTCTACTATTTCAGACTTCGAAAGAAGTTCTAGTCAGGCACAGGATTCTGGGACACACAGCCTTAAGGAAGACAGTAGTGACAGCAGTAGTAAATCTCAAGGAGATATCTTCAGTACCCCATCAAATTTCAAGGCAGCTACCCATTCCTTCATTGTCAAGCGTTCAAATTACTCACCATTGCCCAGTTTTAGCCCTCTTCCAGATGCTTTTAGCAATGTGGTTGATGAAGACACATGGTTGAAAATGAACAAAGGTTTCATAGCTGACCTTGATTCCCTTCAAATGACTCCTGCAAGAAAAGTTCAATCCTTTCCGCCAAGTGACGAAGTTCCTGGCTCAACTGAGAACTACAAGCAGGATGTCCAGAATTTGAAGAGACAACTAGAACTTGCCGTTGGAGAGAGAGATGATCTCAAGAGGAAACATGCTGAACAAGCTGCACTGAACGATCAATTAGTTCGGGATATATCTGAACTACAAAAAGAAGCAAAGCTTATTCGAGAAATCCCTCATAGGCTTTCTGAATGTGCAGCAACTTGCAAAGACTTTTATGTGGATGTGTTATCAAAGACTCAGAGCTTTATATCTGATGAAAAATCTTCGGTGGCAACATTGCTGTCAAGCATAAGTGAAATTGGCACAAACATCTTTACAACTCTCGAAACCCATTTCTCAGAGGCATTTGATGATGGCCAAAGATCATTCTTTGCGAGTAGTTCTCTAATCCAAGAACAGCACAAAGTGCTCTCTGAGAGGTTGAACAGCGCTGTTAAATTATTGGTATCATCAGAGCCATCAAGTGTTGAGAATGAACAAGTGAGGAATTCGCCATGCAGCAGTAAATACAAGGACTACGCAAGGGGAGGAGAAACTGCCGGTTGGGAGGAAAAACTCAGCAATGAGCTCAACACTATCAAGGAAAGATACCGCGGCCTGGAGGAAGAGCTGGATTCGAATAACCAGCTTCTGGAGAAATCCAAGCAAAGATACGATGCCTTGGAAGCTGAATTTCGGCTTTTGAAAGAAGAAAGAGATTCCTTGCACAAAAAGGTATCTGAATCATCTCAAACACTTGCTCTGGTTACCGACCAAAAGGAAAATGTTGTGAAGGATCTGAATCATGAGTTGTTGAGAAGGAAAGATCTTGAAGAACAAATCAAACAGTTCCGTGTTGTTTTTGGTTGTCGGAAGACATCGCTCATGTCTTTCCATAGTGAGTTTAAGTCGAAACTCGAGAGTTTGAGGGCCAAAAATCCAGATTCAGTACCCAAATTTGTTGGATGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000070 GO:0000226 GO:0000278 GO:0000280 GO:0000775 GO:0000776 GO:0000779 GO:0000793 GO:0000819 GO:0001932 GO:0001934 GO:0002376 GO:0002478 GO:0002495 GO:0002504 GO:0003674 GO:0003774 GO:0003777 GO:0003824 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0005737 GO:0005819 GO:0005828 GO:0005829 GO:0005856 GO:0005871 GO:0005874 GO:0005875 GO:0005876 GO:0006810 GO:0006890 GO:0006928 GO:0006996 GO:0007010 GO:0007017 GO:0007018 GO:0007049 GO:0007051 GO:0007052 GO:0007059 GO:0007079 GO:0007080 GO:0007088 GO:0007346 GO:0008017 GO:0008092 GO:0008150 GO:0008608 GO:0009893 GO:0009987 GO:0010562 GO:0010564 GO:0010604 GO:0010965 GO:0015630 GO:0015631 GO:0016043 GO:0016192 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019220 GO:0019222 GO:0019882 GO:0019884 GO:0019886 GO:0022402 GO:0022607 GO:0030071 GO:0030496 GO:0031323 GO:0031325 GO:0031399 GO:0031401 GO:0031974 GO:0031981 GO:0032268 GO:0032270 GO:0032991 GO:0033043 GO:0033044 GO:0033045 GO:0033047 GO:0033674 GO:0034508 GO:0034622 GO:0042325 GO:0042327 GO:0043085 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043515 GO:0043549 GO:0043933 GO:0044085 GO:0044093 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044430 GO:0044444 GO:0044446 GO:0044464 GO:0044877 GO:0045859 GO:0045860 GO:0045937 GO:0048002 GO:0048193 GO:0048285 GO:0048518 GO:0048522 GO:0050000 GO:0050789 GO:0050790 GO:0050794 GO:0051128 GO:0051171 GO:0051173 GO:0051174 GO:0051179 GO:0051233 GO:0051234 GO:0051246 GO:0051247 GO:0051276 GO:0051303 GO:0051305 GO:0051310 GO:0051315 GO:0051338 GO:0051347 GO:0051382 GO:0051383 GO:0051640 GO:0051641 GO:0051649 GO:0051656 GO:0051726 GO:0051783 GO:0051983 GO:0060255 GO:0065003 GO:0065004 GO:0065007 GO:0065009 GO:0070013 GO:0070925 GO:0071824 GO:0071840 GO:0072686 GO:0080090 GO:0098687 GO:0098813 GO:0099080 GO:0099081 GO:0099512 GO:0099513 GO:0099606 GO:0099607 GO:0140014 GO:1901987 GO:1901990 GO:1902099 GO:1902850 GO:1903047 GO:1905818 GO:1990023
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Kinesin

Protein Analysis

894

Amino Acids

100.93

Weight (kDa)

6.6

Isoelectric Point (pI)

50.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Kinesin PF00225 56 - 375 1.5e-110 Kinesin motor domain
Microtub_bd PF16796 86 - 188 2.3e-18 Microtubule binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1021
AccB7I CCANNNNNTGG 1 cut(s) 2362
AccI GTMKAC 2 cut(s) 219, 1003
AccII CGCG 1 cut(s) 2325
AclWI GGATC 4 cut(s) 437, 1228, 1368, 2514
AcoI YGGCCR 2 cut(s) 73, 2086
AcsI RAATTY 8 cut(s) 33, 313, 823, 1519, 1762, 2218, 2402, 2666
AcuI CTGAAG 4 cut(s) 905, 1193, 1199, 1489
AdeI CACNNNGTG 1 cut(s) 2137
AfaI GTAC 7 cut(s) 310, 542, 879, 899, 1071, 1510, 2661
AfeI AGCGCT 1 cut(s) 2161
AfiI CCNNNNNNNGG 6 cut(s) 266, 637, 778, 1019, 2329, 2362
AflII CTTAAG 1 cut(s) 1457
AhlI ACTAGT 1 cut(s) 785
AjnI CCWGG 3 cut(s) 445, 1726, 2328
AjuI GAANNNNNNNTTGG 2 cut(s) 1773, 1805
Alw21I GWGCWC 3 cut(s) 1138, 2142, 2301
Alw26I GTCTC 2 cut(s) 817, 1765
AlwI GGATC 4 cut(s) 437, 1228, 1368, 2514
AlwNI CAGNNNCTG 1 cut(s) 2362
Ama87I CYCGRG 1 cut(s) 2627
Aor51HI AGCGCT 1 cut(s) 2161
AoxI GGCC 6 cut(s) 73, 173, 628, 2086, 2326, 2640
ApeKI GCWGC 7 cut(s) 331, 1163, 1321, 1529, 1832, 1925, 2229
ApoI RAATTY 8 cut(s) 33, 313, 823, 1519, 1762, 2218, 2402, 2666
Asp700I GAANNNNTTC 2 cut(s) 416, 1200
AspLEI GCGC 2 cut(s) 956, 2162
AspS9I GGNCC 1 cut(s) 2640
AsuC2I CCSGG 2 cut(s) 506, 1231
AsuHPI GGTGA 3 cut(s) 328, 412, 1560
AsuII TTCGAA 2 cut(s) 1413, 2348
AsuNHI GCTAGC 1 cut(s) 1106
AvaI CYCGRG 1 cut(s) 2627
AxyI CCTNAGG 1 cut(s) 632
BalI TGGCCA 1 cut(s) 2088
BanII GRGCYC 1 cut(s) 2301
BbsI GAAGAC 4 cut(s) 419, 1470, 1629, 2591
Bbv12I GWGCWC 3 cut(s) 1138, 2142, 2301
BbvI GCAGC 7 cut(s) 318, 1175, 1333, 1541, 1819, 1937, 2241
BccI CCATC 5 cut(s) 104, 944, 1522, 2078, 2197
BceAI ACGGC 5 cut(s) 150, 268, 341, 449, 1772
BcgI CGANNNNNNTGC 2 cut(s) 548, 582
BciT130I CCWGG 3 cut(s) 447, 1728, 2330
BciVI GTATCC 1 cut(s) 535
BcnI CCSGG 2 cut(s) 506, 1231
BcoDI GTCTC 2 cut(s) 817, 1765
BcuI ACTAGT 1 cut(s) 785
BfaI CTAG 7 cut(s) 318, 402, 786, 1107, 1286, 1425, 1778
BfmI CTRYAG 2 cut(s) 1053, 1274
BfoI RGCGCY 1 cut(s) 2163
BfrI CTTAAG 1 cut(s) 1457
BfuAI ACCTGC 1 cut(s) 1021
BfuI GTATCC 1 cut(s) 535
BglII AGATCT 4 cut(s) 150, 737, 1198, 2536
Bme1390I CCNGG 5 cut(s) 447, 506, 1231, 1728, 2330
BmeT110I CYCGRG 1 cut(s) 2627
BmgT120I GGNCC 1 cut(s) 2640
BmiI GGNNCC 2 cut(s) 433, 1093
BmrFI CCNGG 5 cut(s) 447, 506, 1231, 1728, 2330
BmrI ACTGGG 1 cut(s) 1572
BmsI GCATC 5 cut(s) 790, 1131, 1362, 1588, 2377
BmtI GCTAGC 1 cut(s) 1110
BmuI ACTGGG 1 cut(s) 1572
BpiI GAAGAC 4 cut(s) 419, 1470, 1629, 2591
BplI GAGNNNNNCTC 4 cut(s) 1791, 1823, 2098, 2130
BpmI CTGGAG 5 cut(s) 705, 885, 1259, 2351, 2384
Bpu14I TTCGAA 2 cut(s) 1413, 2348
BpuEI CTTGAG 3 cut(s) 1122, 1476, 1793
BpuMI CCSGG 2 cut(s) 506, 1231
BsaBI GATNNNNATC 1 cut(s) 2511
BsaJI CCNNGG 6 cut(s) 250, 339, 1018, 1230, 2323, 2391
BsaWI WCCGGW 3 cut(s) 178, 200, 266
BsaXI ACNNNNNCTCC 2 cut(s) 179, 209
Bsc4I CCNNNNNNNGG 6 cut(s) 266, 637, 778, 1019, 2329, 2362
Bse118I RCCGGY 1 cut(s) 2270
Bse1I ACTGG 3 cut(s) 868, 1317, 1578
Bse21I CCTNAGG 1 cut(s) 632
Bse3DI GCAATG 5 cut(s) 1033, 1571, 1615, 2006, 2299
Bse8I GATNNNNATC 1 cut(s) 2511
BseBI CCWGG 3 cut(s) 447, 1728, 2330
BseDI CCNNGG 6 cut(s) 250, 339, 1018, 1230, 2323, 2391
BseGI GGATG 4 cut(s) 972, 1759, 1957, 2681
BseJI GATNNNNATC 1 cut(s) 2511
BseLI CCNNNNNNNGG 6 cut(s) 266, 637, 778, 1019, 2329, 2362
BseMI GCAATG 5 cut(s) 1033, 1571, 1615, 2006, 2299
BseMII CTCAG 7 cut(s) 646, 1399, 1728, 1982, 2082, 2136, 2302
BseNI ACTGG 3 cut(s) 868, 1317, 1578
BseRI GAGGAG 1 cut(s) 2274
BseXI GCAGC 7 cut(s) 318, 1175, 1333, 1541, 1819, 1937, 2241
BsgI GTGCAG 2 cut(s) 1818, 1944
Bsh1236I CGCG 1 cut(s) 2325
BshFI GGCC 6 cut(s) 75, 175, 630, 2088, 2328, 2642
BsiHKAI GWGCWC 3 cut(s) 1138, 2142, 2301
BsiHKCI CYCGRG 1 cut(s) 2627
BsiSI CCGG 6 cut(s) 179, 201, 267, 505, 1230, 2271
BslFI GGGAC 2 cut(s) 87, 1459
BslI CCNNNNNNNGG 6 cut(s) 266, 637, 778, 1019, 2329, 2362
BsmAI GTCTC 2 cut(s) 817, 1765
BsmFI GGGAC 2 cut(s) 87, 1459
BsmI GAATGC 1 cut(s) 727
BsnI GGCC 6 cut(s) 75, 175, 630, 2088, 2328, 2642
BsoBI CYCGRG 1 cut(s) 2627
Bsp119I TTCGAA 2 cut(s) 1413, 2348
Bsp1286I GDGCHC 3 cut(s) 1138, 2142, 2301
Bsp1407I TGTACA 1 cut(s) 540
BspANI GGCC 6 cut(s) 75, 175, 630, 2088, 2328, 2642
BspCNI CTCAG 7 cut(s) 645, 1398, 1729, 1981, 2081, 2137, 2301
BspFNI CGCG 1 cut(s) 2325
BspHI TCATGA 2 cut(s) 595, 2515
BspLI GGNNCC 2 cut(s) 433, 1093
BspMI ACCTGC 1 cut(s) 1021
BspOI GCTAGC 1 cut(s) 1110
BspPI GGATC 4 cut(s) 437, 1228, 1368, 2514
BspQI GCTCTTC 2 cut(s) 1311, 2331
BspT104I TTCGAA 2 cut(s) 1413, 2348
BspTI CTTAAG 1 cut(s) 1457
BsrDI GCAATG 5 cut(s) 1033, 1571, 1615, 2006, 2299
BsrFI RCCGGY 1 cut(s) 2270
BsrGI TGTACA 1 cut(s) 540
BsrI ACTGG 3 cut(s) 868, 1317, 1578
BssAI RCCGGY 1 cut(s) 2270
BssECI CCNNGG 6 cut(s) 250, 339, 1018, 1230, 2323, 2391
BssNAI GTATAC 1 cut(s) 1004
BssT1I CCWWGG 3 cut(s) 339, 1018, 2391
Bst1107I GTATAC 1 cut(s) 1004
Bst2UI CCWGG 3 cut(s) 447, 1728, 2330
Bst4CI ACNGT 3 cut(s) 1278, 1469, 2559
Bst6I CTCTTC 5 cut(s) 1059, 1311, 1596, 1763, 2331
BstAFI CTTAAG 1 cut(s) 1457
BstAPI GCANNNNNTGC 1 cut(s) 2137
BstAUI TGTACA 1 cut(s) 540
BstBI TTCGAA 2 cut(s) 1413, 2348
BstC8I GCNNGC 5 cut(s) 80, 257, 628, 871, 1108
BstDEI CTNAG 9 cut(s) 632, 944, 1116, 1385, 1737, 1968, 2068, 2145, 2288
BstDSI CCRYGG 2 cut(s) 250, 2323
BstEII GGTNACC 1 cut(s) 2476
BstENI CCTNNNNNAGG 1 cut(s) 635
BstF5I GGATG 4 cut(s) 972, 1759, 1957, 2681
BstFNI CGCG 1 cut(s) 2325
BstH2I RGCGCY 1 cut(s) 2163
BstHHI GCGC 2 cut(s) 956, 2162
BstMAI GTCTC 2 cut(s) 817, 1765
BstMWI GCNNNNNNNGC 6 cut(s) 244, 1112, 1297, 1829, 2137, 2396
BstNI CCWGG 3 cut(s) 447, 1728, 2330
BstNSI RCATGY 2 cut(s) 1229, 1823
BstPI GGTNACC 1 cut(s) 2476
BstSCI CCNGG 5 cut(s) 445, 504, 1229, 1726, 2328
BstSFI CTRYAG 2 cut(s) 1053, 1274
BstUI CGCG 1 cut(s) 2325
BstV1I GCAGC 7 cut(s) 318, 1175, 1333, 1541, 1819, 1937, 2241
BstV2I GAAGAC 4 cut(s) 419, 1470, 1629, 2591
BstX2I RGATCY 6 cut(s) 150, 442, 737, 1198, 2506, 2536
BstYI RGATCY 6 cut(s) 150, 442, 737, 1198, 2506, 2536
BstZ17I GTATAC 1 cut(s) 1004
Bsu36I CCTNAGG 1 cut(s) 632
BsuI GTATCC 1 cut(s) 535
BsuRI GGCC 6 cut(s) 75, 175, 630, 2088, 2328, 2642
BtgI CCRYGG 2 cut(s) 250, 2323
BtgZI GCGATG 1 cut(s) 2574
BtsCI GGATG 4 cut(s) 972, 1759, 1957, 2681
BtsI GCAGTG 1 cut(s) 412
BtsIMutI CAGTG 3 cut(s) 412, 1393, 1835
BveI ACCTGC 1 cut(s) 1021
Cac8I GCNNGC 5 cut(s) 80, 257, 628, 871, 1108
CaiI CAGNNNCTG 1 cut(s) 2362
CciI TCATGA 2 cut(s) 595, 2515
CfoI GCGC 2 cut(s) 956, 2162
Cfr10I RCCGGY 1 cut(s) 2270
Cfr13I GGNCC 1 cut(s) 2640
Cfr42I CCGCGG 1 cut(s) 2326
CseI GACGC 1 cut(s) 1169
Csp6I GTAC 7 cut(s) 309, 541, 878, 898, 1070, 1509, 2660
CspCI CAANNNNNGTGG 2 cut(s) 1369, 1404
CviQI GTAC 7 cut(s) 309, 541, 878, 898, 1070, 1509, 2660
DdeI CTNAG 9 cut(s) 632, 944, 1116, 1385, 1737, 1968, 2068, 2145, 2288
DraIII CACNNNGTG 1 cut(s) 2137
EaeI YGGCCR 2 cut(s) 73, 2086
Eam1104I CTCTTC 5 cut(s) 1059, 1311, 1596, 1763, 2331
EarI CTCTTC 5 cut(s) 1059, 1311, 1596, 1763, 2331
EciI GGCGGA 2 cut(s) 888, 1698
Ecl136II GAGCTC 1 cut(s) 2299
Eco130I CCWWGG 3 cut(s) 339, 1018, 2391
Eco147I AGGCCT 1 cut(s) 175
Eco24I GRGCYC 1 cut(s) 2301
Eco32I GATATC 1 cut(s) 1501
Eco47III AGCGCT 1 cut(s) 2161
Eco53kI GAGCTC 1 cut(s) 2299
Eco57I CTGAAG 4 cut(s) 905, 1193, 1199, 1489
Eco81I CCTNAGG 1 cut(s) 632
Eco88I CYCGRG 1 cut(s) 2627
Eco91I GGTNACC 1 cut(s) 2476
EcoICRI GAGCTC 1 cut(s) 2299
EcoNI CCTNNNNNAGG 1 cut(s) 635
EcoO65I GGTNACC 1 cut(s) 2476
EcoRI GAATTC 2 cut(s) 313, 2218
EcoRII CCWGG 3 cut(s) 445, 1726, 2328
EcoRV GATATC 1 cut(s) 1501
EcoT14I CCWWGG 3 cut(s) 339, 1018, 2391
EcoT22I ATGCAT 1 cut(s) 1355
EcoT38I GRGCYC 1 cut(s) 2301
ErhI CCWWGG 3 cut(s) 339, 1018, 2391
FalI AAGNNNNNCTT 2 cut(s) 1872, 1904
FaqI GGGAC 2 cut(s) 87, 1459
FblI GTMKAC 2 cut(s) 219, 1003
FokI GGATG 3 cut(s) 959, 1766, 1964
FriOI GRGCYC 1 cut(s) 2301
FspBI CTAG 7 cut(s) 318, 402, 786, 1107, 1286, 1425, 1778
GlaI GCGC 2 cut(s) 955, 2161
GsuI CTGGAG 5 cut(s) 705, 885, 1259, 2351, 2384
HaeII RGCGCY 1 cut(s) 2163
HaeIII GGCC 6 cut(s) 75, 175, 630, 2088, 2328, 2642
HapII CCGG 6 cut(s) 179, 201, 267, 505, 1230, 2271
HgaI GACGC 1 cut(s) 1169
HhaI GCGC 2 cut(s) 956, 2162
Hin6I GCGC 2 cut(s) 954, 2160
HinP1I GCGC 2 cut(s) 954, 2160
HincII GTYRAC 1 cut(s) 220
HindII GTYRAC 1 cut(s) 220
HindIII AAGCTT 2 cut(s) 992, 1886
HpaII CCGG 6 cut(s) 179, 201, 267, 505, 1230, 2271
HphI GGTGA 3 cut(s) 328, 412, 1560
Hpy166II GTNNAC 4 cut(s) 23, 220, 1004, 1072
Hpy8I GTNNAC 4 cut(s) 23, 220, 1004, 1072
Hpy99I CGWCG 2 cut(s) 136, 224
HpyCH4III ACNGT 3 cut(s) 1278, 1469, 2559
HpyCH4IV ACGT 1 cut(s) 880
HpyF10VI GCNNNNNNNGC 6 cut(s) 244, 1112, 1297, 1829, 2137, 2396
HpyF3I CTNAG 9 cut(s) 632, 944, 1116, 1385, 1737, 1968, 2068, 2145, 2288
HpySE526I ACGT 1 cut(s) 880
HspAI GCGC 2 cut(s) 954, 2160
KspI CCGCGG 1 cut(s) 2326
LguI GCTCTTC 2 cut(s) 1311, 2331
LmnI GCTCC 5 cut(s) 51, 243, 607, 866, 1240
Lsp1109I GCAGC 7 cut(s) 318, 1175, 1333, 1541, 1819, 1937, 2241
LweI GCATC 5 cut(s) 790, 1131, 1362, 1588, 2377
MaeI CTAG 7 cut(s) 318, 402, 786, 1107, 1286, 1425, 1778
MaeII ACGT 1 cut(s) 880
MaeIII GTNAC 5 cut(s) 498, 968, 1472, 1715, 2476
MflI RGATCY 6 cut(s) 150, 442, 737, 1198, 2506, 2536
MhlI GDGCHC 3 cut(s) 1138, 2142, 2301
MlsI TGGCCA 1 cut(s) 2088
MluNI TGGCCA 1 cut(s) 2088
MlyI GAGTC 2 cut(s) 1675, 1960
MmeI TCCRAC 3 cut(s) 1771, 2561, 2653
Mox20I TGGCCA 1 cut(s) 2088
Mph1103I ATGCAT 1 cut(s) 1355
MroXI GAANNNNTTC 2 cut(s) 416, 1200
MscI TGGCCA 1 cut(s) 2088
MseI TTAA 9 cut(s) 372, 555, 693, 914, 936, 1259, 1458, 2166, 2616
Msp20I TGGCCA 1 cut(s) 2088
MspA1I CMGCKG 2 cut(s) 1324, 2325
MspCI CTTAAG 1 cut(s) 1457
MspI CCGG 6 cut(s) 179, 201, 267, 505, 1230, 2271
MspR9I CCNGG 5 cut(s) 447, 506, 1231, 1728, 2330
Mva1269I GAATGC 1 cut(s) 727
MvaI CCWGG 3 cut(s) 447, 1728, 2330
MvnI CGCG 1 cut(s) 2325
MwoI GCNNNNNNNGC 6 cut(s) 244, 1112, 1297, 1829, 2137, 2396
NciI CCSGG 2 cut(s) 506, 1231
NheI GCTAGC 1 cut(s) 1106
NlaIV GGNNCC 2 cut(s) 433, 1093
NmuCI GTSAC 4 cut(s) 498, 968, 1472, 1715
NsiI ATGCAT 1 cut(s) 1355
NspI RCATGY 2 cut(s) 1229, 1823
NspV TTCGAA 2 cut(s) 1413, 2348
PaeR7I CTCGAG 1 cut(s) 2627
PagI TCATGA 2 cut(s) 595, 2515
PceI AGGCCT 1 cut(s) 175
PciSI GCTCTTC 2 cut(s) 1311, 2331
PctI GAATGC 1 cut(s) 727
PdmI GAANNNNTTC 2 cut(s) 416, 1200
PflMI CCANNNNNTGG 1 cut(s) 2362
PleI GAGTC 2 cut(s) 1675, 1960
PpsI GAGTC 2 cut(s) 1675, 1960
Psp124BI GAGCTC 1 cut(s) 2301
Psp6I CCWGG 3 cut(s) 445, 1726, 2328
PspEI GGTNACC 1 cut(s) 2476
PspGI CCWGG 3 cut(s) 445, 1726, 2328
PspN4I GGNNCC 2 cut(s) 433, 1093
PspPI GGNCC 1 cut(s) 2640
PstNI CAGNNNCTG 1 cut(s) 2362
PsuI RGATCY 6 cut(s) 150, 442, 737, 1198, 2506, 2536
RsaI GTAC 7 cut(s) 310, 542, 879, 899, 1071, 1510, 2661
RsaNI GTAC 7 cut(s) 309, 541, 878, 898, 1070, 1509, 2660
SacI GAGCTC 1 cut(s) 2301
SacII CCGCGG 1 cut(s) 2326
SalI GTCGAC 1 cut(s) 218
SapI GCTCTTC 2 cut(s) 1311, 2331
SaqAI TTAA 9 cut(s) 372, 555, 693, 914, 936, 1259, 1458, 2166, 2616
Sau96I GGNCC 1 cut(s) 2640
SchI GAGTC 2 cut(s) 1675, 1960
ScrFI CCNGG 5 cut(s) 447, 506, 1231, 1728, 2330
SduI GDGCHC 3 cut(s) 1138, 2142, 2301
SfaNI GCATC 5 cut(s) 790, 1131, 1362, 1588, 2377
SfcI CTRYAG 2 cut(s) 1053, 1274
Sfr274I CTCGAG 1 cut(s) 2627
Sfr303I CCGCGG 1 cut(s) 2326
SfuI TTCGAA 2 cut(s) 1413, 2348
SgrBI CCGCGG 1 cut(s) 2326
SlaI CTCGAG 1 cut(s) 2627
SmlI CTYRAG 5 cut(s) 1137, 1457, 1491, 1808, 2627
SmoI CTYRAG 5 cut(s) 1137, 1457, 1491, 1808, 2627
SpeI ACTAGT 1 cut(s) 785
SseBI AGGCCT 1 cut(s) 175
SspI AATATT 2 cut(s) 750, 1251
SspMI CTAG 7 cut(s) 318, 402, 786, 1107, 1286, 1425, 1778
SstI GAGCTC 1 cut(s) 2301
StuI AGGCCT 1 cut(s) 175
StyD4I CCNGG 5 cut(s) 445, 504, 1229, 1726, 2328
StyI CCWWGG 3 cut(s) 339, 1018, 2391
TaaI ACNGT 3 cut(s) 1278, 1469, 2559
TaiI ACGT 1 cut(s) 883
TatI WGTACW 2 cut(s) 540, 897
TauI GCSGC 3 cut(s) 78, 1180, 2328
Tru1I TTAA 9 cut(s) 372, 555, 693, 914, 936, 1259, 1458, 2166, 2616
Tru9I TTAA 9 cut(s) 372, 555, 693, 914, 936, 1259, 1458, 2166, 2616
TscAI CASTG 3 cut(s) 412, 1393, 1842
TseFI GTSAC 4 cut(s) 498, 968, 1472, 1715
TseI GCWGC 7 cut(s) 331, 1163, 1321, 1529, 1832, 1925, 2229
Tsp45I GTSAC 4 cut(s) 498, 968, 1472, 1715
TspGWI ACGGA 1 cut(s) 2552
TspRI CASTG 3 cut(s) 412, 1393, 1842
Van91I CCANNNNNTGG 1 cut(s) 2362
Vha464I CTTAAG 1 cut(s) 1457
XagI CCTNNNNNAGG 1 cut(s) 635
XapI RAATTY 8 cut(s) 33, 313, 823, 1519, 1762, 2218, 2402, 2666
XceI RCATGY 2 cut(s) 1229, 1823
XhoI CTCGAG 1 cut(s) 2627
XmiI GTMKAC 2 cut(s) 219, 1003
XmnI GAANNNNTTC 2 cut(s) 416, 1200
XspI CTAG 7 cut(s) 318, 402, 786, 1107, 1286, 1425, 1778
Zsp2I ATGCAT 1 cut(s) 1355
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.