Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr08

Orthogroup ID

OG0008520

Physical Location & Seq

Genomic Coordinates Reverse (-)

7707440 .. 7709916

Gene Structure 2477 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD08G1092600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 978 bp

ATGGGTGGTTGGGCAATTGCGGTTCACGGCGGAGCGGGTGTCGACCCAAACCTCCCCATGGAGCGTCAGGAGCAGGCCAAACAACTCCTCACGCGGTGCCTCAATCTCGGCATCTCTGCTCTCCGCTCCGATCTCACCGCCATCGATGTTGTTGAACTCGTTGTTCGGGAATTGGAAACCGATCCCTTCTTCAACTCTGGCCGTGGATCCGCTCTCACCGCTAAAGGGACGGTTGAGATGGAAGCCAGCATCATGGACGGACCCAAGAGACGGTGCGGCGCCGTTTCGGGCTTAACCACGGTTAAGAATCCCGTTTCACTCGCCCGCCTCGTCATGGAAAAATCCCCTCATTCCTACCTCGCCTTCTCCGGCGCCGAAGACTTCGCCCGACAACAGGGCGTTGAGGTCGTGGACAATGAGTATTTCATCACGGAGGATAATGTGGGGATGCTCAAATTGGCCAAGGAGGCCAACACAATTTTGTTTGATTATAGGATTCCATTAGGATTGGAGAGTTGCAGTGCAGGCGTAGAGAGTCCGTTGGTCATGAACGGGCTTCCCATTAGAGTTTACGCCCCTGAGACCGTTGGGTGTGTGGTGGTAGATAGCCAAGGGCGTTGCGCTGCAGCTACATCCACCGGTGGGCTCATGAACAAAATGACTGGTCGAATCGGTGACTCGCCACTCATTGGTGCCGGTACCTATGCATGTAACCTTTGCGGGGTCTCGTGCACTGGAGAGGGCGAGGCAATCATACGCGGGACACTCGCGCGTGAGGTGGCAGCAGTTATGGAGTACAAGGGCCTGGGGCTGCAAGATGCAGTGGATTTTGTGATCAAAGAGAGGCTTGATGATGGCAAGGCCGGACTCATAGCTGTGTCTAACAAAGGTGAAGTAGCTTATGGGTTTAATTGTATGGGAATGTTTAGGGGGTGTGCTACCGAGGATGGGTTCATGGAGGTTGAGATTTGGGAGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004067 GO:0004175 GO:0004190 GO:0006508 GO:0006807 GO:0008150 GO:0008152 GO:0008233 GO:0009056 GO:0009057 GO:0016787 GO:0016810 GO:0016811 GO:0019538 GO:0030163 GO:0043170 GO:0044238 GO:0070001 GO:0070011 GO:0071704 GO:0140096 GO:1901564 GO:1901565 GO:1901575

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Asparaginase_2

Protein Analysis

326

Amino Acids

34.48

Weight (kDa)

4.79

Isoelectric Point (pI)

33.96

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Asparaginase_2	PF01112	4 - 321	3.5e-101	Asparaginase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008520)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G16150
fragaria_vesca	FvH4_2g33770
malus_domestica	MD08G1092600.v1.1 MD15G1077000.v1.1
prunus_persica	Prupe.1G430400_v2.0.a1 Prupe.1G430400_v2.0.a1 Prupe.1G430400_v2.0.a1
pyrus_communis	pycom08g07530 pycom15g07170
rosa_chinensis	RchiOBHm_Chr4g0445551 RchiOBHm_Chr6g0308781
rosa_laevigata	RLG00000010619
rosa_multiflora	Rmu_sc0000871.1_g000036 Rmu_sc0009346.1_g000001 Rmu_sc0027152.1_g000001 Rmu_sc0027153.1_g000001 Rmu_sc0027154.1_g000001
rosa_roxburghii	Rroxscaffold_7G00159940
rosa_rugosa	Rorug06G0368100
rosa_samantha	Rh4DG432200 Rh6CG494400 Rh6DG479700
rosa_wichuraiana	Rw6G041790

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	1 cut(s)	698
AccB1I	GGYRCC	5 cut(s)	96, 278, 371, 692, 698
AccB7I	CCANNNNNTGG	1 cut(s)	689
AccBSI	CCGCTC	3 cut(s)	35, 126, 212
AccI	GTMKAC	1 cut(s)	42
AccII	CGCG	4 cut(s)	94, 759, 770, 772
AclWI	GGATC	3 cut(s)	176, 201, 214
AcoI	YGGCCR	2 cut(s)	199, 459
AcyI	GRCGYC	2 cut(s)	279, 372
AdeI	CACNNNGTG	1 cut(s)	96
AfaI	GTAC	2 cut(s)	700, 797
AfiI	CCNNNNNNNGG	9 cut(s)	58, 225, 270, 334, 394, 642, 689, 721, 948
AgeI	ACCGGT	1 cut(s)	638
AgsI	TTSAA	2 cut(s)	155, 193
AjnI	CCWGG	1 cut(s)	804
AluBI	AGCT	3 cut(s)	629, 875, 899
AluI	AGCT	3 cut(s)	629, 875, 899
Alw21I	GWGCWC	1 cut(s)	734
Alw26I	GTCTC	3 cut(s)	262, 575, 730
Alw44I	GTGCAC	1 cut(s)	730
AlwI	GGATC	3 cut(s)	176, 201, 214
AoxI	GGCC	6 cut(s)	75, 199, 459, 468, 802, 861
ApaLI	GTGCAC	1 cut(s)	730
ApeKI	GCWGC	4 cut(s)	623, 626, 782, 811
AsiGI	ACCGGT	1 cut(s)	638
Asp718I	GGTACC	1 cut(s)	698
AspLEI	GCGC	4 cut(s)	281, 374, 623, 772
AspS9I	GGNCC	2 cut(s)	260, 802
AsuHPI	GGTGA	4 cut(s)	127, 208, 686, 902
AvaII	GGWCC	1 cut(s)	260
BaeGI	GKGCMC	1 cut(s)	734
BalI	TGGCCA	1 cut(s)	461
BamHI	GGATCC	1 cut(s)	206
BanI	GGYRCC	5 cut(s)	96, 278, 371, 692, 698
BanII	GRGCYC	1 cut(s)	648
BauI	CACGAG	1 cut(s)	727
BbsI	GAAGAC	1 cut(s)	384
Bbv12I	GWGCWC	1 cut(s)	734
BbvI	GCAGC	4 cut(s)	610, 638, 794, 798
BccI	CCATC	4 cut(s)	149, 232, 848, 941
BceAI	ACGGC	3 cut(s)	43, 186, 266
BciT130I	CCWGG	1 cut(s)	806
BclI	TGATCA	1 cut(s)	834
BcoDI	GTCTC	3 cut(s)	262, 575, 730
BfmI	CTRYAG	1 cut(s)	624
BfoI	RGCGCY	2 cut(s)	282, 375
BglI	GCCNNNNNGGC	1 cut(s)	467
BisI	GCNGC	5 cut(s)	277, 624, 627, 783, 812
BlsI	GCNGC	5 cut(s)	278, 625, 628, 784, 813
Bme1390I	CCNGG	1 cut(s)	806
Bme18I	GGWCC	1 cut(s)	260
BmgT120I	GGNCC	2 cut(s)	260, 802
BmiI	GGNNCC	7 cut(s)	98, 208, 262, 280, 373, 694, 700
BmrFI	CCNGG	1 cut(s)	806
BmsI	GCATC	4 cut(s)	120, 258, 438, 808
BpiI	GAAGAC	1 cut(s)	384
BpmI	CTGGAG	1 cut(s)	756
Bsa29I	ATCGAT	1 cut(s)	144
BsaHI	GRCGYC	2 cut(s)	279, 372
BsaI	GGTCTC	2 cut(s)	575, 730
BsaJI	CCNNGG	7 cut(s)	57, 202, 297, 462, 610, 805, 942
BsaWI	WCCGGW	1 cut(s)	638
BsaXI	ACNNNNNCTCC	2 cut(s)	24, 54
Bsc4I	CCNNNNNNNGG	9 cut(s)	58, 225, 270, 334, 394, 642, 689, 721, 948
Bse118I	RCCGGY	2 cut(s)	638, 695
Bse1I	ACTGG	2 cut(s)	667, 739
BseBI	CCWGG	1 cut(s)	806
BseCI	ATCGAT	1 cut(s)	144
BseDI	CCNNGG	7 cut(s)	57, 202, 297, 462, 610, 805, 942
BseGI	GGATG	3 cut(s)	453, 632, 952
BseLI	CCNNNNNNNGG	9 cut(s)	58, 225, 270, 334, 394, 642, 689, 721, 948
BseMII	CTCAG	1 cut(s)	570
BseNI	ACTGG	2 cut(s)	667, 739
BseRI	GAGGAG	1 cut(s)	77
BseSI	GKGCMC	1 cut(s)	734
BseXI	GCAGC	4 cut(s)	610, 638, 794, 798
BsgI	GTGCAG	1 cut(s)	543
Bsh1236I	CGCG	4 cut(s)	94, 759, 770, 772
BshFI	GGCC	6 cut(s)	77, 201, 461, 470, 804, 863
BshNI	GGYRCC	5 cut(s)	96, 278, 371, 692, 698
BshTI	ACCGGT	1 cut(s)	638
BshVI	ATCGAT	1 cut(s)	144
BsiHKAI	GWGCWC	1 cut(s)	734
BsiSI	CCGG	4 cut(s)	369, 639, 696, 864
BslFI	GGGAC	2 cut(s)	241, 775
BslI	CCNNNNNNNGG	9 cut(s)	58, 225, 270, 334, 394, 642, 689, 721, 948
BsmAI	GTCTC	3 cut(s)	262, 575, 730
BsmBI	CGTCTC	1 cut(s)	262
BsmFI	GGGAC	2 cut(s)	241, 775
BsnI	GGCC	6 cut(s)	77, 201, 461, 470, 804, 863
Bso31I	GGTCTC	2 cut(s)	575, 730
Bsp1286I	GDGCHC	2 cut(s)	648, 734
Bsp143I	GATC	4 cut(s)	130, 181, 206, 834
Bsp19I	CCATGG	1 cut(s)	57
BspANI	GGCC	6 cut(s)	77, 201, 461, 470, 804, 863
BspCNI	CTCAG	1 cut(s)	571
BspDI	ATCGAT	1 cut(s)	144
BspFNI	CGCG	4 cut(s)	94, 759, 770, 772
BspHI	TCATGA	2 cut(s)	546, 648
BspLI	GGNNCC	7 cut(s)	98, 208, 262, 280, 373, 694, 700
BspMAI	CTGCAG	1 cut(s)	628
BspPI	GGATC	3 cut(s)	176, 201, 214
BspT107I	GGYRCC	5 cut(s)	96, 278, 371, 692, 698
BspTNI	GGTCTC	2 cut(s)	575, 730
BsrBI	CCGCTC	3 cut(s)	35, 126, 212
BsrFI	RCCGGY	2 cut(s)	638, 695
BsrI	ACTGG	2 cut(s)	667, 739
BssAI	RCCGGY	2 cut(s)	638, 695
BssECI	CCNNGG	7 cut(s)	57, 202, 297, 462, 610, 805, 942
BssMI	GATC	4 cut(s)	130, 181, 206, 834
BssNI	GRCGYC	2 cut(s)	279, 372
BssSI	CACGAG	1 cut(s)	727
BssT1I	CCWWGG	3 cut(s)	57, 462, 610
Bst2BI	CACGAG	1 cut(s)	727
Bst2UI	CCWGG	1 cut(s)	806
Bst4CI	ACNGT	4 cut(s)	232, 273, 301, 586
BstACI	GRCGYC	2 cut(s)	279, 372
BstC8I	GCNNGC	4 cut(s)	75, 247, 325, 526
BstDEI	CTNAG	1 cut(s)	579
BstDSI	CCRYGG	3 cut(s)	57, 202, 297
BstF5I	GGATG	3 cut(s)	453, 632, 952
BstFNI	CGCG	4 cut(s)	94, 759, 770, 772
BstH2I	RGCGCY	2 cut(s)	282, 375
BstHHI	GCGC	4 cut(s)	281, 374, 623, 772
BstKTI	GATC	4 cut(s)	133, 184, 209, 837
BstMAI	GTCTC	3 cut(s)	262, 575, 730
BstMBI	GATC	4 cut(s)	130, 181, 206, 834
BstMWI	GCNNNNNNNGC	4 cut(s)	70, 218, 467, 525
BstNI	CCWGG	1 cut(s)	806
BstNSI	RCATGY	1 cut(s)	711
BstSCI	CCNGG	1 cut(s)	804
BstSFI	CTRYAG	1 cut(s)	624
BstSLI	GKGCMC	1 cut(s)	734
BstUI	CGCG	4 cut(s)	94, 759, 770, 772
BstV1I	GCAGC	4 cut(s)	610, 638, 794, 798
BstV2I	GAAGAC	1 cut(s)	384
BstX2I	RGATCY	1 cut(s)	206
BstXI	CCANNNNNNTGG	1 cut(s)	253
BstYI	RGATCY	1 cut(s)	206
Bsu15I	ATCGAT	1 cut(s)	144
BsuRI	GGCC	6 cut(s)	77, 201, 461, 470, 804, 863
BsuTUI	ATCGAT	1 cut(s)	144
BtgI	CCRYGG	3 cut(s)	57, 202, 297
BtsCI	GGATG	3 cut(s)	453, 632, 952
BtsI	GCAGTG	2 cut(s)	526, 828
BtsIMutI	CAGTG	3 cut(s)	526, 732, 828
Cac8I	GCNNGC	4 cut(s)	75, 247, 325, 526
CciI	TCATGA	2 cut(s)	546, 648
CfoI	GCGC	4 cut(s)	281, 374, 623, 772
Cfr10I	RCCGGY	2 cut(s)	638, 695
Cfr13I	GGNCC	2 cut(s)	260, 802
ClaI	ATCGAT	1 cut(s)	144
CseI	GACGC	1 cut(s)	53
Csp6I	GTAC	2 cut(s)	699, 796
CspAI	ACCGGT	1 cut(s)	638
CviAII	CATG	7 cut(s)	58, 253, 334, 547, 649, 708, 955
CviQI	GTAC	2 cut(s)	699, 796
DdeI	CTNAG	1 cut(s)	579
DinI	GGCGCC	2 cut(s)	280, 373
DpnI	GATC	4 cut(s)	132, 183, 208, 836
DpnII	GATC	4 cut(s)	130, 181, 206, 834
DraIII	CACNNNGTG	1 cut(s)	96
EaeI	YGGCCR	2 cut(s)	199, 459
EciI	GGCGGA	1 cut(s)	45
Eco130I	CCWWGG	3 cut(s)	57, 462, 610
Eco24I	GRGCYC	1 cut(s)	648
Eco31I	GGTCTC	2 cut(s)	575, 730
Eco47I	GGWCC	1 cut(s)	260
EcoO109I	RGGNCCY	1 cut(s)	802
EcoRII	CCWGG	1 cut(s)	804
EcoT14I	CCWWGG	3 cut(s)	57, 462, 610
EcoT22I	ATGCAT	1 cut(s)	709
EcoT38I	GRGCYC	1 cut(s)	648
EgeI	GGCGCC	2 cut(s)	280, 373
EheI	GGCGCC	2 cut(s)	280, 373
ErhI	CCWWGG	3 cut(s)	57, 462, 610
Esp3I	CGTCTC	1 cut(s)	262
FaeI	CATG	7 cut(s)	61, 256, 337, 550, 652, 711, 958
FalI	AAGNNNNNCTT	2 cut(s)	831, 863
FaqI	GGGAC	2 cut(s)	241, 775
FatI	CATG	7 cut(s)	57, 252, 333, 546, 648, 707, 954
FauI	CCCGC	4 cut(s)	28, 332, 713, 752
FbaI	TGATCA	1 cut(s)	834
FblI	GTMKAC	1 cut(s)	42
Fnu4HI	GCNGC	5 cut(s)	277, 624, 627, 783, 812
FokI	GGATG	3 cut(s)	460, 619, 959
FriOI	GRGCYC	1 cut(s)	648
Fsp4HI	GCNGC	5 cut(s)	277, 624, 627, 783, 812
GlaI	GCGC	4 cut(s)	280, 373, 622, 771
GluI	GCNGC	5 cut(s)	277, 624, 627, 783, 812
GsuI	CTGGAG	1 cut(s)	756
HaeII	RGCGCY	2 cut(s)	282, 375
HaeIII	GGCC	6 cut(s)	77, 201, 461, 470, 804, 863
HapII	CCGG	4 cut(s)	369, 639, 696, 864
HgaI	GACGC	1 cut(s)	53
HhaI	GCGC	4 cut(s)	281, 374, 623, 772
Hin1I	GRCGYC	2 cut(s)	279, 372
Hin1II	CATG	7 cut(s)	61, 256, 337, 550, 652, 711, 958
Hin6I	GCGC	4 cut(s)	279, 372, 621, 770
HinP1I	GCGC	4 cut(s)	279, 372, 621, 770
HincII	GTYRAC	1 cut(s)	43
HindII	GTYRAC	1 cut(s)	43
HinfI	GANTC	6 cut(s)	307, 496, 535, 669, 677, 867
HpaII	CCGG	4 cut(s)	369, 639, 696, 864
HphI	GGTGA	4 cut(s)	127, 208, 686, 902
Hpy166II	GTNNAC	5 cut(s)	25, 43, 412, 571, 732
Hpy188I	TCNGA	1 cut(s)	130
Hpy188III	TCNNGA	4 cut(s)	68, 167, 547, 649
Hpy8I	GTNNAC	5 cut(s)	25, 43, 412, 571, 732
HpyAV	CCTTC	2 cut(s)	196, 373
HpyCH4III	ACNGT	4 cut(s)	232, 273, 301, 586
HpyCH4V	TGCA	7 cut(s)	519, 524, 626, 707, 732, 814, 821
HpyF10VI	GCNNNNNNNGC	4 cut(s)	70, 218, 467, 525
HpyF3I	CTNAG	1 cut(s)	579
Hsp92I	GRCGYC	2 cut(s)	279, 372
Hsp92II	CATG	7 cut(s)	61, 256, 337, 550, 652, 711, 958
HspAI	GCGC	4 cut(s)	279, 372, 621, 770
KasI	GGCGCC	2 cut(s)	278, 371
KpnI	GGTACC	1 cut(s)	702
Ksp22I	TGATCA	1 cut(s)	834
Kzo9I	GATC	4 cut(s)	130, 181, 206, 834
LmnI	GCTCC	4 cut(s)	32, 61, 70, 131
Lsp1109I	GCAGC	4 cut(s)	610, 638, 794, 798
LweI	GCATC	4 cut(s)	120, 258, 438, 808
MaeIII	GTNAC	2 cut(s)	674, 710
MalI	GATC	4 cut(s)	132, 183, 208, 836
MbiI	CCGCTC	3 cut(s)	35, 126, 212
MboI	GATC	4 cut(s)	130, 181, 206, 834
MboII	GAAGA	2 cut(s)	181, 389
MfeI	CAATTG	1 cut(s)	15
MflI	RGATCY	1 cut(s)	206
MhlI	GDGCHC	2 cut(s)	648, 734
MlsI	TGGCCA	1 cut(s)	461
MluCI	AATT	5 cut(s)	15, 170, 455, 477, 910
MluNI	TGGCCA	1 cut(s)	461
Mly113I	GGCGCC	2 cut(s)	279, 372
MlyI	GAGTC	3 cut(s)	544, 671, 861
Mox20I	TGGCCA	1 cut(s)	461
Mph1103I	ATGCAT	1 cut(s)	709
MscI	TGGCCA	1 cut(s)	461
MseI	TTAA	3 cut(s)	293, 303, 909
MslI	CAYNNNNRTG	1 cut(s)	875
Msp20I	TGGCCA	1 cut(s)	461
MspI	CCGG	4 cut(s)	369, 639, 696, 864
MspR9I	CCNGG	1 cut(s)	806
MunI	CAATTG	1 cut(s)	15
MvaI	CCWGG	1 cut(s)	806
MvnI	CGCG	4 cut(s)	94, 759, 770, 772
MwoI	GCNNNNNNNGC	4 cut(s)	70, 218, 467, 525
NarI	GGCGCC	2 cut(s)	279, 372
NcoI	CCATGG	1 cut(s)	57
NdeII	GATC	4 cut(s)	130, 181, 206, 834
NlaIII	CATG	7 cut(s)	61, 256, 337, 550, 652, 711, 958
NlaIV	GGNNCC	7 cut(s)	98, 208, 262, 280, 373, 694, 700
NmeAIII	GCCGAG	1 cut(s)	87
NmuCI	GTSAC	1 cut(s)	674
NsiI	ATGCAT	1 cut(s)	709
NspI	RCATGY	1 cut(s)	711
PagI	TCATGA	2 cut(s)	546, 648
PcsI	WCGNNNNNNNCGW	1 cut(s)	327
PfeI	GAWTC	3 cut(s)	307, 496, 669
PflMI	CCANNNNNTGG	1 cut(s)	689
PinAI	ACCGGT	1 cut(s)	638
PkrI	GCNGC	5 cut(s)	278, 625, 628, 784, 813
PleI	GAGTC	3 cut(s)	543, 671, 861
PluTI	GGCGCC	2 cut(s)	282, 375
PpsI	GAGTC	3 cut(s)	543, 671, 861
Psp6I	CCWGG	1 cut(s)	804
PspGI	CCWGG	1 cut(s)	804
PspN4I	GGNNCC	7 cut(s)	98, 208, 262, 280, 373, 694, 700
PspPI	GGNCC	2 cut(s)	260, 802
PstI	CTGCAG	1 cut(s)	628
PsuI	RGATCY	1 cut(s)	206
RsaI	GTAC	2 cut(s)	700, 797
RsaNI	GTAC	2 cut(s)	699, 796
RseI	CAYNNNNRTG	1 cut(s)	875
SalI	GTCGAC	1 cut(s)	41
SaqAI	TTAA	3 cut(s)	293, 303, 909
SatI	GCNGC	5 cut(s)	277, 624, 627, 783, 812
Sau3AI	GATC	4 cut(s)	130, 181, 206, 834
Sau96I	GGNCC	2 cut(s)	260, 802
SchI	GAGTC	3 cut(s)	544, 671, 861
ScrFI	CCNGG	1 cut(s)	806
SduI	GDGCHC	2 cut(s)	648, 734
SfaNI	GCATC	4 cut(s)	120, 258, 438, 808
SfcI	CTRYAG	1 cut(s)	624
SfiI	GGCCNNNNNGGCC	1 cut(s)	467
SfoI	GGCGCC	2 cut(s)	280, 373
SgrAI	CRCCGGYG	1 cut(s)	638
SinI	GGWCC	1 cut(s)	260
SmiMI	CAYNNNNRTG	1 cut(s)	875
Sse9I	AATT	5 cut(s)	15, 170, 455, 477, 910
SspDI	GGCGCC	2 cut(s)	278, 371
StyD4I	CCNGG	1 cut(s)	804
StyI	CCWWGG	3 cut(s)	57, 462, 610
TaaI	ACNGT	4 cut(s)	232, 273, 301, 586
TaqI	TCGA	3 cut(s)	42, 144, 667
TasI	AATT	5 cut(s)	15, 170, 455, 477, 910
TatI	WGTACW	1 cut(s)	795
TauI	GCSGC	1 cut(s)	279
TfiI	GAWTC	3 cut(s)	307, 496, 669
Tru1I	TTAA	3 cut(s)	293, 303, 909
Tru9I	TTAA	3 cut(s)	293, 303, 909
TscAI	CASTG	3 cut(s)	526, 739, 828
TseFI	GTSAC	1 cut(s)	674
TseI	GCWGC	4 cut(s)	623, 626, 782, 811
Tsp45I	GTSAC	1 cut(s)	674
TspDTI	ATGAA	4 cut(s)	415, 563, 665, 943
TspGWI	ACGGA	3 cut(s)	273, 446, 528
TspRI	CASTG	3 cut(s)	526, 739, 828
Van91I	CCANNNNNTGG	1 cut(s)	689
VneI	GTGCAC	1 cut(s)	730
VpaK11BI	GGWCC	1 cut(s)	260
XceI	RCATGY	1 cut(s)	711
XmiI	GTMKAC	1 cut(s)	42
Zsp2I	ATGCAT	1 cut(s)	709

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.