Rh6CG494400

isoaspartyl peptidase L-asparaginase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6C
OG0008520
Physical Location & Seq
Reverse (-)
66013801 .. 66016153
2353 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6CG494400.1

Sequence Viewer

Length: 984 bp
ATGGGCGGTTGGGCTATTGCTGTGCACGGTGGCGCTGGCGTCGACCCAAATCTCCCTCTCGAGCGCCAAGAACAAGCCAAACAGCTCCTCACTCGCTGCCTCAATCTCGGAATCTCTGCTCTCCGTTCTGATCTTCCCGCCATTGATGTTGTTGAACTTGTTGTTAGGGAATTGGAAACGGATCCGTTTTTCAATTCCGGCCGTGGATCCGCCCTGAATGAAAATGGCAGGGTTGAGATGGAAGCCAGCATCATGGATGGACCCAAAAGACGATGCGGCGCCGTATCGGGGTTGAGCACCGTCAAGAACCCGATCTCGCTTGCCCGACTCGTCATGGATAAGTCCCCGCATTCCTACCTTGCCTTCTCCGGCGCCGAAAGCTTCGCCAGGCAACAGGGCGTTGAGCTGGTGGACAATGACTATTTTATCACGGAGGAGAATGTGGGGATGCTCAAGTTGGCCAAGGAAGCCAACACCATCTTGTTTGATTACAGAATCCCACTTGGGATGGAGACTTGCGCTGCTGGTGTGGAGAGCCCGTGCCTCCAGATGAACGGGCTGCCCATAAGCGTGTACGCACCCGAGACGGTCGGATGTGTGGTCGTGGACAGCCAGGGCCGCTGCGCAGCTGCCACCTCTACCGGTGGGCTCATGAACAAAATGACAGGTCGCATTGGGGACTCGCCACTCATTGGGGCTGGTACCTACGCGTGTGACCTCTGTGGGATCTCTTGCACAGGAGAGGGAGAGGCCATCATACGCGGGACACTGGCGCGTGAAGTTGCTGCTGTGATGGAGTACAAGGGCTTAGGCCTTCAGGAGGCTGTGGACTTTGTGATAAAGAACAGGCTCGATGAAGGAAAGGCCGGACTCATTGCTGTGTCCAACAAAGGAGAAGTGGCTTGTGGGTTTAACTGTTGCGGAATGTTCAGGGGGAGTGCAACGGAGGATGGGTTCATGGAGGTTGGGATTTGGGATCAGTAG

Protein Analysis

327

Amino Acids

34.59

Weight (kDa)

4.75

Isoelectric Point (pI)

34.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Asparaginase_2 PF01112 4 - 324 1.4e-99 Asparaginase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 625
Acc65I GGTACC 1 cut(s) 701
AccB1I GGYRCC 3 cut(s) 278, 371, 701
AccB7I CCANNNNNTGG 1 cut(s) 692
AccI GTMKAC 1 cut(s) 42
AccII CGCG 3 cut(s) 710, 762, 775
AciI CCGC 8 cut(s) 6, 138, 210, 276, 347, 619, 762, 921
AclWI GGATC 5 cut(s) 176, 189, 201, 214, 734
AcoI YGGCCR 2 cut(s) 199, 459
AcuI CTGAAG 1 cut(s) 800
AcyI GRCGYC 3 cut(s) 39, 279, 372
AfaI GTAC 3 cut(s) 575, 703, 800
AfiI CCNNNNNNNGG 3 cut(s) 288, 692, 820
AflIII ACRYGT 1 cut(s) 708
AgeI ACCGGT 1 cut(s) 641
AgsI TTSAA 2 cut(s) 155, 193
AjnI CCWGG 2 cut(s) 386, 612
AloI GAACNNNNNNTCC 2 cut(s) 938, 970
AluBI AGCT 4 cut(s) 85, 381, 406, 629
AluI AGCT 4 cut(s) 85, 381, 406, 629
Alw21I GWGCWC 2 cut(s) 27, 299
Alw26I GTCTC 2 cut(s) 506, 578
Alw44I GTGCAC 1 cut(s) 23
AlwI GGATC 5 cut(s) 176, 189, 201, 214, 734
Ama87I CYCGRG 2 cut(s) 59, 581
AoxI GGCC 6 cut(s) 199, 459, 616, 750, 811, 864
ApaLI GTGCAC 1 cut(s) 23
ApeKI GCWGC 7 cut(s) 96, 521, 559, 621, 626, 629, 785
AsiGI ACCGGT 1 cut(s) 641
Asp718I GGTACC 1 cut(s) 701
AspLEI GCGC 7 cut(s) 35, 66, 281, 374, 521, 626, 775
AspS9I GGNCC 2 cut(s) 260, 616
AvaI CYCGRG 2 cut(s) 59, 581
AvaII GGWCC 1 cut(s) 260
BaeGI GKGCMC 1 cut(s) 27
BalI TGGCCA 1 cut(s) 461
BamHI GGATCC 2 cut(s) 181, 206
BanI GGYRCC 3 cut(s) 278, 371, 701
BanII GRGCYC 2 cut(s) 539, 651
Bbv12I GWGCWC 2 cut(s) 27, 299
BbvI GCAGC 7 cut(s) 83, 508, 546, 608, 616, 638, 772
BccI CCATC 7 cut(s) 232, 251, 485, 502, 761, 787, 944
BceAI ACGGC 2 cut(s) 186, 266
BciT130I CCWGG 2 cut(s) 388, 614
BcoDI GTCTC 2 cut(s) 506, 578
BfoI RGCGCY 4 cut(s) 36, 67, 282, 375
BisI GCNGC 9 cut(s) 97, 277, 522, 560, 619, 622, 627, 630, 786
BlsI GCNGC 9 cut(s) 98, 278, 523, 561, 620, 623, 628, 631, 787
Bme1390I CCNGG 2 cut(s) 388, 614
Bme18I GGWCC 1 cut(s) 260
BmeT110I CYCGRG 2 cut(s) 59, 581
BmgT120I GGNCC 2 cut(s) 260, 616
BmiI GGNNCC 6 cut(s) 183, 208, 262, 280, 373, 703
BmrFI CCNGG 2 cut(s) 388, 614
BmsI GCATC 3 cut(s) 258, 263, 438
BpmI CTGGAG 1 cut(s) 530
Bpu10I CCTNAGC 1 cut(s) 808
BpuEI CTTGAG 1 cut(s) 437
BsaHI GRCGYC 3 cut(s) 39, 279, 372
BsaJI CCNNGG 3 cut(s) 202, 462, 613
BsaWI WCCGGW 1 cut(s) 641
BsaXI ACNNNNNCTCC 4 cut(s) 524, 554, 938, 968
Bsc4I CCNNNNNNNGG 3 cut(s) 288, 692, 820
Bse118I RCCGGY 1 cut(s) 641
Bse1I ACTGG 1 cut(s) 774
Bse3DI GCAATG 1 cut(s) 873
BseBI CCWGG 2 cut(s) 388, 614
BseDI CCNNGG 3 cut(s) 202, 462, 613
BseGI GGATG 5 cut(s) 262, 453, 513, 599, 955
BseLI CCNNNNNNNGG 3 cut(s) 288, 692, 820
BseMI GCAATG 1 cut(s) 873
BseNI ACTGG 1 cut(s) 774
BseRI GAGGAG 2 cut(s) 77, 449
BseSI GKGCMC 1 cut(s) 27
BseX3I CGGCCG 1 cut(s) 199
BseXI GCAGC 7 cut(s) 83, 508, 546, 608, 616, 638, 772
Bsh1236I CGCG 3 cut(s) 710, 762, 775
Bsh1285I CGRYCG 2 cut(s) 202, 591
BshFI GGCC 6 cut(s) 201, 461, 618, 752, 813, 866
BshNI GGYRCC 3 cut(s) 278, 371, 701
BshTI ACCGGT 1 cut(s) 641
BsiEI CGRYCG 2 cut(s) 202, 591
BsiHKAI GWGCWC 2 cut(s) 27, 299
BsiHKCI CYCGRG 2 cut(s) 59, 581
BsiSI CCGG 4 cut(s) 198, 369, 642, 867
BslFI GGGAC 3 cut(s) 328, 692, 778
BslI CCNNNNNNNGG 3 cut(s) 288, 692, 820
BsmAI GTCTC 2 cut(s) 506, 578
BsmBI CGTCTC 1 cut(s) 578
BsmFI GGGAC 3 cut(s) 328, 692, 778
BsmI GAATGC 1 cut(s) 349
BsnI GGCC 6 cut(s) 201, 461, 618, 752, 813, 866
BsoBI CYCGRG 2 cut(s) 59, 581
Bsp1286I GDGCHC 4 cut(s) 27, 299, 539, 651
Bsp143I GATC 6 cut(s) 130, 181, 206, 312, 726, 976
BspACI CCGC 8 cut(s) 6, 138, 210, 276, 347, 619, 762, 921
BspANI GGCC 6 cut(s) 201, 461, 618, 752, 813, 866
BspFNI CGCG 3 cut(s) 710, 762, 775
BspHI TCATGA 1 cut(s) 651
BspLI GGNNCC 6 cut(s) 183, 208, 262, 280, 373, 703
BspPI GGATC 5 cut(s) 176, 189, 201, 214, 734
BspT107I GGYRCC 3 cut(s) 278, 371, 701
BsrDI GCAATG 1 cut(s) 873
BsrFI RCCGGY 1 cut(s) 641
BsrI ACTGG 1 cut(s) 774
BssAI RCCGGY 1 cut(s) 641
BssECI CCNNGG 3 cut(s) 202, 462, 613
BssMI GATC 6 cut(s) 130, 181, 206, 312, 726, 976
BssNI GRCGYC 3 cut(s) 39, 279, 372
BssT1I CCWWGG 1 cut(s) 462
Bst2UI CCWGG 2 cut(s) 388, 614
Bst4CI ACNGT 4 cut(s) 29, 301, 589, 917
BstACI GRCGYC 3 cut(s) 39, 279, 372
BstC8I GCNNGC 3 cut(s) 37, 247, 321
BstDEI CTNAG 1 cut(s) 808
BstDSI CCRYGG 1 cut(s) 202
BstENI CCTNNNNNAGG 1 cut(s) 818
BstF5I GGATG 5 cut(s) 262, 453, 513, 599, 955
BstFNI CGCG 3 cut(s) 710, 762, 775
BstH2I RGCGCY 4 cut(s) 36, 67, 282, 375
BstHHI GCGC 7 cut(s) 35, 66, 281, 374, 521, 626, 775
BstKTI GATC 6 cut(s) 133, 184, 209, 315, 729, 979
BstMAI GTCTC 2 cut(s) 506, 578
BstMBI GATC 6 cut(s) 130, 181, 206, 312, 726, 976
BstMCI CGRYCG 2 cut(s) 202, 591
BstMWI GCNNNNNNNGC 3 cut(s) 378, 467, 618
BstNI CCWGG 2 cut(s) 388, 614
BstSCI CCNGG 2 cut(s) 386, 612
BstSLI GKGCMC 1 cut(s) 27
BstUI CGCG 3 cut(s) 710, 762, 775
BstV1I GCAGC 7 cut(s) 83, 508, 546, 608, 616, 638, 772
BstX2I RGATCY 3 cut(s) 181, 206, 726
BstXI CCANNNNNNTGG 1 cut(s) 253
BstYI RGATCY 3 cut(s) 181, 206, 726
BstZI CGGCCG 1 cut(s) 199
BsuRI GGCC 6 cut(s) 201, 461, 618, 752, 813, 866
BtgI CCRYGG 1 cut(s) 202
BtsCI GGATG 5 cut(s) 262, 453, 513, 599, 955
BtsIMutI CAGTG 1 cut(s) 767
Cac8I GCNNGC 3 cut(s) 37, 247, 321
CciI TCATGA 1 cut(s) 651
CfoI GCGC 7 cut(s) 35, 66, 281, 374, 521, 626, 775
Cfr10I RCCGGY 1 cut(s) 641
Cfr13I GGNCC 2 cut(s) 260, 616
CseI GACGC 1 cut(s) 28
Csp6I GTAC 3 cut(s) 574, 702, 799
CspAI ACCGGT 1 cut(s) 641
CviAII CATG 4 cut(s) 253, 334, 652, 958
CviQI GTAC 3 cut(s) 574, 702, 799
DdeI CTNAG 1 cut(s) 808
DinI GGCGCC 2 cut(s) 280, 373
DpnI GATC 6 cut(s) 132, 183, 208, 314, 728, 978
DpnII GATC 6 cut(s) 130, 181, 206, 312, 726, 976
EaeI YGGCCR 2 cut(s) 199, 459
EagI CGGCCG 1 cut(s) 199
EciI GGCGGA 1 cut(s) 199
EclXI CGGCCG 1 cut(s) 199
Eco130I CCWWGG 1 cut(s) 462
Eco147I AGGCCT 1 cut(s) 813
Eco24I GRGCYC 2 cut(s) 539, 651
Eco47I GGWCC 1 cut(s) 260
Eco52I CGGCCG 1 cut(s) 199
Eco57I CTGAAG 1 cut(s) 800
Eco88I CYCGRG 2 cut(s) 59, 581
EcoNI CCTNNNNNAGG 1 cut(s) 818
EcoRII CCWGG 2 cut(s) 386, 612
EcoT14I CCWWGG 1 cut(s) 462
EcoT38I GRGCYC 2 cut(s) 539, 651
EgeI GGCGCC 2 cut(s) 280, 373
EheI GGCGCC 2 cut(s) 280, 373
ErhI CCWWGG 1 cut(s) 462
Esp3I CGTCTC 1 cut(s) 578
FaeI CATG 4 cut(s) 256, 337, 655, 961
FaiI YATR 6 cut(s) 254, 335, 566, 653, 758, 959
FaqI GGGAC 3 cut(s) 328, 692, 778
FatI CATG 4 cut(s) 252, 333, 651, 957
FauI CCCGC 3 cut(s) 145, 354, 755
FblI GTMKAC 1 cut(s) 42
Fnu4HI GCNGC 9 cut(s) 97, 277, 522, 560, 619, 622, 627, 630, 786
FokI GGATG 5 cut(s) 269, 460, 520, 606, 962
FriOI GRGCYC 2 cut(s) 539, 651
Fsp4HI GCNGC 9 cut(s) 97, 277, 522, 560, 619, 622, 627, 630, 786
FspI TGCGCA 1 cut(s) 625
GlaI GCGC 7 cut(s) 34, 65, 280, 373, 520, 625, 774
GluI GCNGC 9 cut(s) 97, 277, 522, 560, 619, 622, 627, 630, 786
GsuI CTGGAG 1 cut(s) 530
HaeII RGCGCY 4 cut(s) 36, 67, 282, 375
HaeIII GGCC 6 cut(s) 201, 461, 618, 752, 813, 866
HapII CCGG 4 cut(s) 198, 369, 642, 867
HgaI GACGC 1 cut(s) 28
HhaI GCGC 7 cut(s) 35, 66, 281, 374, 521, 626, 775
Hin1I GRCGYC 3 cut(s) 39, 279, 372
Hin1II CATG 4 cut(s) 256, 337, 655, 961
Hin6I GCGC 7 cut(s) 33, 64, 279, 372, 519, 624, 773
HinP1I GCGC 7 cut(s) 33, 64, 279, 372, 519, 624, 773
HincII GTYRAC 1 cut(s) 43
HindII GTYRAC 1 cut(s) 43
HindIII AAGCTT 1 cut(s) 379
HinfI GANTC 5 cut(s) 111, 327, 495, 680, 870
HpaII CCGG 4 cut(s) 198, 369, 642, 867
Hpy166II GTNNAC 6 cut(s) 25, 43, 412, 574, 607, 829
Hpy188I TCNGA 3 cut(s) 110, 130, 593
Hpy188III TCNNGA 5 cut(s) 59, 304, 547, 652, 818
Hpy8I GTNNAC 6 cut(s) 25, 43, 412, 574, 607, 829
Hpy99I CGWCG 1 cut(s) 44
HpyAV CCTTC 3 cut(s) 373, 824, 851
HpyCH4III ACNGT 4 cut(s) 29, 301, 589, 917
HpyCH4V TGCA 3 cut(s) 25, 735, 941
HpyF10VI GCNNNNNNNGC 3 cut(s) 378, 467, 618
HpyF3I CTNAG 1 cut(s) 808
Hsp92I GRCGYC 3 cut(s) 39, 279, 372
Hsp92II CATG 4 cut(s) 256, 337, 655, 961
HspAI GCGC 7 cut(s) 33, 64, 279, 372, 519, 624, 773
KasI GGCGCC 2 cut(s) 278, 371
KpnI GGTACC 1 cut(s) 705
Kzo9I GATC 6 cut(s) 130, 181, 206, 312, 726, 976
LmnI GCTCC 1 cut(s) 90
Lsp1109I GCAGC 7 cut(s) 83, 508, 546, 608, 616, 638, 772
LweI GCATC 3 cut(s) 258, 263, 438
MaeIII GTNAC 1 cut(s) 713
MalI GATC 6 cut(s) 132, 183, 208, 314, 728, 978
MboI GATC 6 cut(s) 130, 181, 206, 312, 726, 976
MboII GAAGA 1 cut(s) 125
MflI RGATCY 3 cut(s) 181, 206, 726
MhlI GDGCHC 4 cut(s) 27, 299, 539, 651
MlsI TGGCCA 1 cut(s) 461
MluCI AATT 2 cut(s) 170, 193
MluI ACGCGT 1 cut(s) 708
MluNI TGGCCA 1 cut(s) 461
Mly113I GGCGCC 2 cut(s) 279, 372
MlyI GAGTC 3 cut(s) 321, 674, 864
MmeI TCCRAC 2 cut(s) 571, 909
Mox20I TGGCCA 1 cut(s) 461
MscI TGGCCA 1 cut(s) 461
MseI TTAA 1 cut(s) 912
MslI CAYNNNNRTG 2 cut(s) 569, 878
Msp20I TGGCCA 1 cut(s) 461
MspA1I CMGCKG 2 cut(s) 621, 629
MspI CCGG 4 cut(s) 198, 369, 642, 867
MspR9I CCNGG 2 cut(s) 388, 614
Mva1269I GAATGC 1 cut(s) 349
MvaI CCWGG 2 cut(s) 388, 614
MvnI CGCG 3 cut(s) 710, 762, 775
MwoI GCNNNNNNNGC 3 cut(s) 378, 467, 618
NarI GGCGCC 2 cut(s) 279, 372
NdeII GATC 6 cut(s) 130, 181, 206, 312, 726, 976
NlaIII CATG 4 cut(s) 256, 337, 655, 961
NlaIV GGNNCC 6 cut(s) 183, 208, 262, 280, 373, 703
NmuCI GTSAC 1 cut(s) 713
NsbI TGCGCA 1 cut(s) 625
PaeR7I CTCGAG 1 cut(s) 59
PagI TCATGA 1 cut(s) 651
PceI AGGCCT 1 cut(s) 813
PctI GAATGC 1 cut(s) 349
PfeI GAWTC 2 cut(s) 111, 495
PflMI CCANNNNNTGG 1 cut(s) 692
PinAI ACCGGT 1 cut(s) 641
PkrI GCNGC 9 cut(s) 98, 278, 523, 561, 620, 623, 628, 631, 787
PleI GAGTC 3 cut(s) 321, 674, 864
PluTI GGCGCC 2 cut(s) 282, 375
PpsI GAGTC 3 cut(s) 321, 674, 864
Psp6I CCWGG 2 cut(s) 386, 612
PspGI CCWGG 2 cut(s) 386, 612
PspN4I GGNNCC 6 cut(s) 183, 208, 262, 280, 373, 703
PspPI GGNCC 2 cut(s) 260, 616
PsuI RGATCY 3 cut(s) 181, 206, 726
PvuII CAGCTG 1 cut(s) 629
RsaI GTAC 3 cut(s) 575, 703, 800
RsaNI GTAC 3 cut(s) 574, 702, 799
RseI CAYNNNNRTG 2 cut(s) 569, 878
SalI GTCGAC 1 cut(s) 41
SaqAI TTAA 1 cut(s) 912
SatI GCNGC 9 cut(s) 97, 277, 522, 560, 619, 622, 627, 630, 786
Sau3AI GATC 6 cut(s) 130, 181, 206, 312, 726, 976
Sau96I GGNCC 2 cut(s) 260, 616
SchI GAGTC 3 cut(s) 321, 674, 864
ScrFI CCNGG 2 cut(s) 388, 614
SduI GDGCHC 4 cut(s) 27, 299, 539, 651
SfaNI GCATC 3 cut(s) 258, 263, 438
SfoI GGCGCC 2 cut(s) 280, 373
Sfr274I CTCGAG 1 cut(s) 59
SinI GGWCC 1 cut(s) 260
SlaI CTCGAG 1 cut(s) 59
SmiMI CAYNNNNRTG 2 cut(s) 569, 878
SmlI CTYRAG 2 cut(s) 59, 452
SmoI CTYRAG 2 cut(s) 59, 452
Sse9I AATT 2 cut(s) 170, 193
SseBI AGGCCT 1 cut(s) 813
SsiI CCGC 8 cut(s) 6, 138, 210, 276, 347, 619, 762, 921
SspDI GGCGCC 2 cut(s) 278, 371
StuI AGGCCT 1 cut(s) 813
StyD4I CCNGG 2 cut(s) 386, 612
StyI CCWWGG 1 cut(s) 462
TaaI ACNGT 4 cut(s) 29, 301, 589, 917
TaqI TCGA 3 cut(s) 42, 60, 852
TasI AATT 2 cut(s) 170, 193
TatI WGTACW 1 cut(s) 798
TauI GCSGC 2 cut(s) 279, 621
TfiI GAWTC 2 cut(s) 111, 495
Tru1I TTAA 1 cut(s) 912
Tru9I TTAA 1 cut(s) 912
TscAI CASTG 1 cut(s) 774
TseFI GTSAC 1 cut(s) 713
TseI GCWGC 7 cut(s) 96, 521, 559, 621, 626, 629, 785
Tsp45I GTSAC 1 cut(s) 713
TspDTI ATGAA 5 cut(s) 234, 566, 668, 870, 946
TspGWI ACGGA 5 cut(s) 113, 174, 194, 446, 959
TspRI CASTG 1 cut(s) 774
Van91I CCANNNNNTGG 1 cut(s) 692
VneI GTGCAC 1 cut(s) 23
VpaK11BI GGWCC 1 cut(s) 260
XagI CCTNNNNNAGG 1 cut(s) 818
XhoI CTCGAG 1 cut(s) 59
XmiI GTMKAC 1 cut(s) 42
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.