Rh4DG432200

isoaspartyl peptidase L-asparaginase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0008520
Physical Location & Seq
Forward (+)
63632936 .. 63634233
1298 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG432200.1

Sequence Viewer

Length: 981 bp
ATGGGCGGTTGGGCTATTGCTGTGCACGGTGGCGCTGGTGTCGACCCAAATCTCCCTCTCGAGCGCCAAGAACAAGCCAAACAGCTCGTCACTCGCTGCCTCAATCTCGGAATCTCTGCTCTCCGTTCTGATCTTCCCGCCATTGATGTTGTTGAACTTGTTGTTAGGGAATTGGAAACGGATCCGTTTTTCAATTCCTGCCGTGGATCCGCCCTGAATGAAAATGGCAGGGTTGAGATGGAAGCCAGCATCATGGATGGACCCAAAAGACGATGCGGCGCCGTATCGAGGTTGAGCACCGTCAAGAACCCGATCTCGCTTGCCCGACTCGTCATGGATAAGTCCCCGCATTCCTACCTTGCCTTCTCCGGCGCAGAAAGCTTCGCCAGGCAACAGGGCGTTGAGCTGGTGGACAATGACTATTTTATCACGGAGGAGAATGTGGGGATGCTCAAGTTGGCCAAGGAAGCCAACACCATCTTGTTTGATTACAGAATTCCACTTGGGATGGAGACTTGCGCTGCTGGTGTGGAGAGCCCGATACAGATGAACGGGCTGCCCATAAGCGTGTACGCACCCGAGACGGTCGGATGTGTGGTCGTTGACAGCCAGGGCCGCTGCGCAGCTGCCACCTCTACCAGTGGGCTCATGAACAAAATGACAGGTCGCATTGGGGACTCGCCACTCATCGGGGCTGATACCTACGCGTGTGACCTCTGTGGGGTCTCTTGCACAGGAGAGGGAGAGGCCATCATACGCGGGACACTGGCGCGTGAAGTTTCTGCTGTGATGGAGTACAAGGGCTTAGGCCTTCAGGACGCTGTGGACTTTGTGATCAAGAACAGGCTCGATGAAGGAAAGGCCGGACTCATTGCTGTGTCCAACAAAGGAGAAGTGGCTTGTGGGTTTAACTGCTGCGGAATGTTCAGGGGGAGTGCAACAGAGGATGGGTTCATGGAGGTTGGGATTTGGGATCAGTAG

Protein Analysis

326

Amino Acids

34.69

Weight (kDa)

4.75

Isoelectric Point (pI)

34.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Asparaginase_2 PF01112 4 - 323 1.8e-95 Asparaginase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 622
AccB1I GGYRCC 1 cut(s) 278
AccI GTMKAC 1 cut(s) 42
AccII CGCG 3 cut(s) 707, 759, 772
AciI CCGC 8 cut(s) 6, 138, 210, 276, 347, 616, 759, 918
AclWI GGATC 4 cut(s) 176, 189, 201, 214
AcoI YGGCCR 1 cut(s) 459
AcsI RAATTY 1 cut(s) 495
AcuI CTGAAG 1 cut(s) 797
AcyI GRCGYC 1 cut(s) 279
AfaI GTAC 2 cut(s) 572, 797
AfiI CCNNNNNNNGG 3 cut(s) 288, 689, 721
AflIII ACRYGT 1 cut(s) 705
AgsI TTSAA 2 cut(s) 155, 193
AjnI CCWGG 2 cut(s) 386, 609
AluBI AGCT 4 cut(s) 85, 381, 406, 626
AluI AGCT 4 cut(s) 85, 381, 406, 626
Alw21I GWGCWC 2 cut(s) 27, 299
Alw26I GTCTC 3 cut(s) 506, 575, 730
Alw44I GTGCAC 1 cut(s) 23
AlwI GGATC 4 cut(s) 176, 189, 201, 214
Ama87I CYCGRG 2 cut(s) 59, 578
AoxI GGCC 5 cut(s) 459, 613, 747, 808, 861
ApaLI GTGCAC 1 cut(s) 23
ApeKI GCWGC 7 cut(s) 96, 521, 556, 618, 623, 626, 915
ApoI RAATTY 1 cut(s) 495
ArsI GACNNNNNNTTYG 2 cut(s) 72, 104
AspLEI GCGC 7 cut(s) 35, 66, 281, 374, 521, 623, 772
AspS9I GGNCC 2 cut(s) 260, 613
AvaI CYCGRG 2 cut(s) 59, 578
AvaII GGWCC 1 cut(s) 260
BaeGI GKGCMC 1 cut(s) 27
BalI TGGCCA 1 cut(s) 461
BamHI GGATCC 2 cut(s) 181, 206
BanI GGYRCC 1 cut(s) 278
BanII GRGCYC 2 cut(s) 539, 648
Bbv12I GWGCWC 2 cut(s) 27, 299
BbvI GCAGC 7 cut(s) 83, 508, 543, 605, 613, 635, 902
BccI CCATC 7 cut(s) 232, 251, 485, 502, 758, 784, 941
BceAI ACGGC 2 cut(s) 186, 266
BciT130I CCWGG 2 cut(s) 388, 611
BclI TGATCA 1 cut(s) 834
BcoDI GTCTC 3 cut(s) 506, 575, 730
BfoI RGCGCY 3 cut(s) 36, 67, 282
BisI GCNGC 9 cut(s) 97, 277, 522, 557, 616, 619, 624, 627, 916
BlsI GCNGC 9 cut(s) 98, 278, 523, 558, 617, 620, 625, 628, 917
Bme1390I CCNGG 2 cut(s) 388, 611
Bme18I GGWCC 1 cut(s) 260
BmeT110I CYCGRG 2 cut(s) 59, 578
BmgT120I GGNCC 2 cut(s) 260, 613
BmiI GGNNCC 4 cut(s) 183, 208, 262, 280
BmrFI CCNGG 2 cut(s) 388, 611
BmsI GCATC 3 cut(s) 258, 263, 438
Bpu10I CCTNAGC 1 cut(s) 805
BpuEI CTTGAG 1 cut(s) 437
BsaHI GRCGYC 1 cut(s) 279
BsaI GGTCTC 1 cut(s) 730
BsaJI CCNNGG 3 cut(s) 202, 462, 610
Bsc4I CCNNNNNNNGG 3 cut(s) 288, 689, 721
Bse1I ACTGG 2 cut(s) 639, 771
Bse3DI GCAATG 1 cut(s) 870
BseBI CCWGG 2 cut(s) 388, 611
BseDI CCNNGG 3 cut(s) 202, 462, 610
BseGI GGATG 5 cut(s) 262, 453, 513, 596, 952
BseLI CCNNNNNNNGG 3 cut(s) 288, 689, 721
BseMI GCAATG 1 cut(s) 870
BseNI ACTGG 2 cut(s) 639, 771
BseRI GAGGAG 1 cut(s) 449
BseSI GKGCMC 1 cut(s) 27
BseXI GCAGC 7 cut(s) 83, 508, 543, 605, 613, 635, 902
Bsh1236I CGCG 3 cut(s) 707, 759, 772
Bsh1285I CGRYCG 1 cut(s) 588
BshFI GGCC 5 cut(s) 461, 615, 749, 810, 863
BshNI GGYRCC 1 cut(s) 278
BsiEI CGRYCG 1 cut(s) 588
BsiHKAI GWGCWC 2 cut(s) 27, 299
BsiHKCI CYCGRG 2 cut(s) 59, 578
BsiSI CCGG 2 cut(s) 369, 864
BslFI GGGAC 3 cut(s) 328, 689, 775
BslI CCNNNNNNNGG 3 cut(s) 288, 689, 721
BsmAI GTCTC 3 cut(s) 506, 575, 730
BsmBI CGTCTC 1 cut(s) 575
BsmFI GGGAC 3 cut(s) 328, 689, 775
BsmI GAATGC 1 cut(s) 349
BsnI GGCC 5 cut(s) 461, 615, 749, 810, 863
Bso31I GGTCTC 1 cut(s) 730
BsoBI CYCGRG 2 cut(s) 59, 578
Bsp1286I GDGCHC 4 cut(s) 27, 299, 539, 648
Bsp143I GATC 6 cut(s) 130, 181, 206, 312, 834, 973
BspACI CCGC 8 cut(s) 6, 138, 210, 276, 347, 616, 759, 918
BspANI GGCC 5 cut(s) 461, 615, 749, 810, 863
BspFNI CGCG 3 cut(s) 707, 759, 772
BspHI TCATGA 1 cut(s) 648
BspLI GGNNCC 4 cut(s) 183, 208, 262, 280
BspPI GGATC 4 cut(s) 176, 189, 201, 214
BspT107I GGYRCC 1 cut(s) 278
BspTNI GGTCTC 1 cut(s) 730
BsrDI GCAATG 1 cut(s) 870
BsrI ACTGG 2 cut(s) 639, 771
BssECI CCNNGG 3 cut(s) 202, 462, 610
BssMI GATC 6 cut(s) 130, 181, 206, 312, 834, 973
BssNI GRCGYC 1 cut(s) 279
BssT1I CCWWGG 1 cut(s) 462
Bst2UI CCWGG 2 cut(s) 388, 611
Bst4CI ACNGT 3 cut(s) 29, 301, 586
BstACI GRCGYC 1 cut(s) 279
BstC8I GCNNGC 2 cut(s) 247, 321
BstDEI CTNAG 1 cut(s) 805
BstDSI CCRYGG 1 cut(s) 202
BstF5I GGATG 5 cut(s) 262, 453, 513, 596, 952
BstFNI CGCG 3 cut(s) 707, 759, 772
BstH2I RGCGCY 3 cut(s) 36, 67, 282
BstHHI GCGC 7 cut(s) 35, 66, 281, 374, 521, 623, 772
BstKTI GATC 6 cut(s) 133, 184, 209, 315, 837, 976
BstMAI GTCTC 3 cut(s) 506, 575, 730
BstMBI GATC 6 cut(s) 130, 181, 206, 312, 834, 973
BstMCI CGRYCG 1 cut(s) 588
BstMWI GCNNNNNNNGC 3 cut(s) 378, 467, 615
BstNI CCWGG 2 cut(s) 388, 611
BstSCI CCNGG 2 cut(s) 386, 609
BstSLI GKGCMC 1 cut(s) 27
BstUI CGCG 3 cut(s) 707, 759, 772
BstV1I GCAGC 7 cut(s) 83, 508, 543, 605, 613, 635, 902
BstX2I RGATCY 2 cut(s) 181, 206
BstXI CCANNNNNNTGG 1 cut(s) 253
BstYI RGATCY 2 cut(s) 181, 206
BsuRI GGCC 5 cut(s) 461, 615, 749, 810, 863
BtgI CCRYGG 1 cut(s) 202
BtsCI GGATG 5 cut(s) 262, 453, 513, 596, 952
BtsIMutI CAGTG 2 cut(s) 646, 764
Cac8I GCNNGC 2 cut(s) 247, 321
CciI TCATGA 1 cut(s) 648
CfoI GCGC 7 cut(s) 35, 66, 281, 374, 521, 623, 772
Cfr13I GGNCC 2 cut(s) 260, 613
CseI GACGC 1 cut(s) 827
Csp6I GTAC 2 cut(s) 571, 796
CviAII CATG 4 cut(s) 253, 334, 649, 955
CviQI GTAC 2 cut(s) 571, 796
DdeI CTNAG 1 cut(s) 805
DinI GGCGCC 1 cut(s) 280
DpnI GATC 6 cut(s) 132, 183, 208, 314, 836, 975
DpnII GATC 6 cut(s) 130, 181, 206, 312, 834, 973
EaeI YGGCCR 1 cut(s) 459
EciI GGCGGA 1 cut(s) 199
Eco130I CCWWGG 1 cut(s) 462
Eco147I AGGCCT 1 cut(s) 810
Eco24I GRGCYC 2 cut(s) 539, 648
Eco31I GGTCTC 1 cut(s) 730
Eco47I GGWCC 1 cut(s) 260
Eco57I CTGAAG 1 cut(s) 797
Eco88I CYCGRG 2 cut(s) 59, 578
EcoRI GAATTC 1 cut(s) 495
EcoRII CCWGG 2 cut(s) 386, 609
EcoT14I CCWWGG 1 cut(s) 462
EcoT38I GRGCYC 2 cut(s) 539, 648
EgeI GGCGCC 1 cut(s) 280
EheI GGCGCC 1 cut(s) 280
ErhI CCWWGG 1 cut(s) 462
Esp3I CGTCTC 1 cut(s) 575
FaeI CATG 4 cut(s) 256, 337, 652, 958
FaiI YATR 6 cut(s) 254, 335, 563, 650, 755, 956
FaqI GGGAC 3 cut(s) 328, 689, 775
FatI CATG 4 cut(s) 252, 333, 648, 954
FauI CCCGC 3 cut(s) 145, 354, 752
FbaI TGATCA 1 cut(s) 834
FblI GTMKAC 1 cut(s) 42
Fnu4HI GCNGC 9 cut(s) 97, 277, 522, 557, 616, 619, 624, 627, 916
FokI GGATG 5 cut(s) 269, 460, 520, 603, 959
FriOI GRGCYC 2 cut(s) 539, 648
Fsp4HI GCNGC 9 cut(s) 97, 277, 522, 557, 616, 619, 624, 627, 916
FspI TGCGCA 1 cut(s) 622
GlaI GCGC 7 cut(s) 34, 65, 280, 373, 520, 622, 771
GluI GCNGC 9 cut(s) 97, 277, 522, 557, 616, 619, 624, 627, 916
HaeII RGCGCY 3 cut(s) 36, 67, 282
HaeIII GGCC 5 cut(s) 461, 615, 749, 810, 863
HapII CCGG 2 cut(s) 369, 864
HgaI GACGC 1 cut(s) 827
HhaI GCGC 7 cut(s) 35, 66, 281, 374, 521, 623, 772
Hin1I GRCGYC 1 cut(s) 279
Hin1II CATG 4 cut(s) 256, 337, 652, 958
Hin6I GCGC 7 cut(s) 33, 64, 279, 372, 519, 621, 770
HinP1I GCGC 7 cut(s) 33, 64, 279, 372, 519, 621, 770
HincII GTYRAC 2 cut(s) 43, 604
HindII GTYRAC 2 cut(s) 43, 604
HindIII AAGCTT 1 cut(s) 379
HinfI GANTC 4 cut(s) 111, 327, 677, 867
HpaII CCGG 2 cut(s) 369, 864
Hpy166II GTNNAC 6 cut(s) 25, 43, 412, 571, 604, 826
Hpy188I TCNGA 3 cut(s) 110, 130, 590
Hpy188III TCNNGA 5 cut(s) 59, 304, 649, 815, 838
Hpy8I GTNNAC 6 cut(s) 25, 43, 412, 571, 604, 826
HpyAV CCTTC 3 cut(s) 373, 821, 848
HpyCH4III ACNGT 3 cut(s) 29, 301, 586
HpyCH4V TGCA 3 cut(s) 25, 732, 938
HpyF10VI GCNNNNNNNGC 3 cut(s) 378, 467, 615
HpyF3I CTNAG 1 cut(s) 805
Hsp92I GRCGYC 1 cut(s) 279
Hsp92II CATG 4 cut(s) 256, 337, 652, 958
HspAI GCGC 7 cut(s) 33, 64, 279, 372, 519, 621, 770
KasI GGCGCC 1 cut(s) 278
Ksp22I TGATCA 1 cut(s) 834
Kzo9I GATC 6 cut(s) 130, 181, 206, 312, 834, 973
Lsp1109I GCAGC 7 cut(s) 83, 508, 543, 605, 613, 635, 902
LweI GCATC 3 cut(s) 258, 263, 438
MaeIII GTNAC 2 cut(s) 88, 710
MalI GATC 6 cut(s) 132, 183, 208, 314, 836, 975
MboI GATC 6 cut(s) 130, 181, 206, 312, 834, 973
MboII GAAGA 1 cut(s) 125
MflI RGATCY 2 cut(s) 181, 206
MhlI GDGCHC 4 cut(s) 27, 299, 539, 648
MlsI TGGCCA 1 cut(s) 461
MluCI AATT 3 cut(s) 170, 193, 495
MluI ACGCGT 1 cut(s) 705
MluNI TGGCCA 1 cut(s) 461
Mly113I GGCGCC 1 cut(s) 279
MlyI GAGTC 3 cut(s) 321, 671, 861
MmeI TCCRAC 2 cut(s) 568, 906
Mox20I TGGCCA 1 cut(s) 461
MscI TGGCCA 1 cut(s) 461
MseI TTAA 1 cut(s) 909
MslI CAYNNNNRTG 2 cut(s) 566, 875
Msp20I TGGCCA 1 cut(s) 461
MspA1I CMGCKG 2 cut(s) 618, 626
MspI CCGG 2 cut(s) 369, 864
MspR9I CCNGG 2 cut(s) 388, 611
Mva1269I GAATGC 1 cut(s) 349
MvaI CCWGG 2 cut(s) 388, 611
MvnI CGCG 3 cut(s) 707, 759, 772
MwoI GCNNNNNNNGC 3 cut(s) 378, 467, 615
NarI GGCGCC 1 cut(s) 279
NdeII GATC 6 cut(s) 130, 181, 206, 312, 834, 973
NlaIII CATG 4 cut(s) 256, 337, 652, 958
NlaIV GGNNCC 4 cut(s) 183, 208, 262, 280
NmuCI GTSAC 2 cut(s) 88, 710
NsbI TGCGCA 1 cut(s) 622
PaeR7I CTCGAG 1 cut(s) 59
PagI TCATGA 1 cut(s) 648
PceI AGGCCT 1 cut(s) 810
PctI GAATGC 1 cut(s) 349
PfeI GAWTC 1 cut(s) 111
PkrI GCNGC 9 cut(s) 98, 278, 523, 558, 617, 620, 625, 628, 917
PleI GAGTC 3 cut(s) 321, 671, 861
PluTI GGCGCC 1 cut(s) 282
PpsI GAGTC 3 cut(s) 321, 671, 861
Psp6I CCWGG 2 cut(s) 386, 609
PspGI CCWGG 2 cut(s) 386, 609
PspN4I GGNNCC 4 cut(s) 183, 208, 262, 280
PspPI GGNCC 2 cut(s) 260, 613
PsuI RGATCY 2 cut(s) 181, 206
PvuII CAGCTG 1 cut(s) 626
RsaI GTAC 2 cut(s) 572, 797
RsaNI GTAC 2 cut(s) 571, 796
RseI CAYNNNNRTG 2 cut(s) 566, 875
SalI GTCGAC 1 cut(s) 41
SaqAI TTAA 1 cut(s) 909
SatI GCNGC 9 cut(s) 97, 277, 522, 557, 616, 619, 624, 627, 916
Sau3AI GATC 6 cut(s) 130, 181, 206, 312, 834, 973
Sau96I GGNCC 2 cut(s) 260, 613
SchI GAGTC 3 cut(s) 321, 671, 861
ScrFI CCNGG 2 cut(s) 388, 611
SduI GDGCHC 4 cut(s) 27, 299, 539, 648
SfaNI GCATC 3 cut(s) 258, 263, 438
SfoI GGCGCC 1 cut(s) 280
Sfr274I CTCGAG 1 cut(s) 59
SinI GGWCC 1 cut(s) 260
SlaI CTCGAG 1 cut(s) 59
SmiMI CAYNNNNRTG 2 cut(s) 566, 875
SmlI CTYRAG 2 cut(s) 59, 452
SmoI CTYRAG 2 cut(s) 59, 452
Sse9I AATT 3 cut(s) 170, 193, 495
SseBI AGGCCT 1 cut(s) 810
SsiI CCGC 8 cut(s) 6, 138, 210, 276, 347, 616, 759, 918
SspDI GGCGCC 1 cut(s) 278
StuI AGGCCT 1 cut(s) 810
StyD4I CCNGG 2 cut(s) 386, 609
StyI CCWWGG 1 cut(s) 462
TaaI ACNGT 3 cut(s) 29, 301, 586
TaqI TCGA 4 cut(s) 42, 60, 287, 849
TasI AATT 3 cut(s) 170, 193, 495
TatI WGTACW 1 cut(s) 795
TauI GCSGC 2 cut(s) 279, 618
TfiI GAWTC 1 cut(s) 111
Tru1I TTAA 1 cut(s) 909
Tru9I TTAA 1 cut(s) 909
TscAI CASTG 2 cut(s) 646, 771
TseFI GTSAC 2 cut(s) 88, 710
TseI GCWGC 7 cut(s) 96, 521, 556, 618, 623, 626, 915
Tsp45I GTSAC 2 cut(s) 88, 710
TspDTI ATGAA 5 cut(s) 234, 563, 665, 867, 943
TspGWI ACGGA 4 cut(s) 113, 174, 194, 446
TspRI CASTG 2 cut(s) 646, 771
VneI GTGCAC 1 cut(s) 23
VpaK11BI GGWCC 1 cut(s) 260
XapI RAATTY 1 cut(s) 495
XhoI CTCGAG 1 cut(s) 59
XmiI GTMKAC 1 cut(s) 42
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.