MD08G1095100.v1.1
NAC Family

inactive receptor kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0011041
Physical Location & Seq
Forward (+)
7982347 .. 7983817
1471 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1095100.v1.1.491

Sequence Viewer

Length: 1431 bp
ATGTTCAAGAAATGGCACATCTTTTTACTTCTTCTCTCACTCTCAACCTCCACTGTTACTTCCTCTAGGCCTTCAGGCACGCAACCATCACTCCCACCCGACGCTCTCGCCCTCGTCGCCTTCAAATCCAAAGCGGATTTACACGGCGCCCTTCCATTCTCCTCCAACGCCACCGCCTTCCAGTCCTTCTGCCGCTGGACTGGCGTCCAATGCGGCGCCCGGTACAAACTAGTCCGCTTCATAGTCCAGTCCCAAAACCTCGGCGGCATTTTCGCGCCCGACACCCTGACTCGGTTAGACCAGCTCAGAGTCCTGAGTCTGCAGAACAACTCGCTCACCGGGCCGCTTCCAGACCTCACCGGACTCACCAACCTCAAAACGCTCTTCCTCGACCACAACTCCTTCGCCGGTTCCCTCCCGCCATCGCTCTCCTCCCTCCACCGCCTCCGAACCCTCGATTTCTCGTACAACAACCTCACCGGAACACTACCCACATTTCTAATCACTGGTCTGGACCGGCTGTACTACCTCCGGCTGGATTGGAACCGGTTCAGTGGACCGGTCCCGCCTCTAAACCAGTCCACGCTCCAGACATTCAATGTCTCCGGAAACAACCTCACCGGCGCAATCCCCGTCACTCCGACTCTGCTGCGGTTCGGTGCATCATCCTTCTCGTGGAACCCAGGCCTCTGCGGCGAGATTGTGAATAAGGAATGCAACCGGACGAGACCCTTTTTCGGCGCAACTCATGTTCACGAAGCTCCGCCGCCCACGAGGGTGCACGGGCAGAGCAGTGCAGAAAATATCCACGGCGTTGAGTTGACTCAGCCGAGTCACAAAATCCCAAGAAGAAACGCCCTCATTATCGGGTTTTCCACTGGCGTCTTCGTCCTGATCTGCTCGCTCCTCTGTTTCGCAATCGCGGTGAAGAAACAGAGGAAATCCCAAACCCGAAAAGCCGTTAATTCTGGGGGACCAACTGCGGCGGAGGATACGGCGGCAGCGGTGGTGGAAATAGAGGAGGAGCTGGAGCAGAAGGTGAAGAGGGCGCAGGGGATTCAAGTAGTGAAGAGCGGGAGCCTAATGTTCTGCGCGGGCGAGTCGCAGCTGTACTCGCTGGACCAGCTGATGCGGGCGTCGGCGGAGCTGCTGGGGAAGGGGACCATTGGGACGACATACAAGGCGGTGCTGGACAACCGCTTGATCGTGAGCGTGAAGAGGCTCGATGCCGGGAAGTTGAGTGGGACTAGCAGAGAGGTGTTCGAGCGGCATCTGGAGTCGGTGGGTGCGCTGAGACACCCGAATCTGGTGCCGCTGAGAGCGTATTTTCAGGCGAAGGACGAGCGGCTGCTGGTGTACGATTACCAGCCCAACGGCAGCGTCTTCTCTCTCGTTCACGGTAATGTTCAAATCTTTCAGTTGAGTGGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

477

Amino Acids

52.06

Weight (kDa)

9.66

Isoelectric Point (pI)

37.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 96 - 183 3.1e-06 Leucine-rich repeat region
Pkinase PF00069 382 - 468 1.3e-08 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011041)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G43020
fragaria_vesca FvH4_2g33931 FvH4_2g33931
malus_domestica MD08G1095100.v1.1 MD15G1078000.v1.1
prunus_persica Prupe.1G431300_v2.0.a1
pyrus_communis pycom08g07710 pycom15g07280
rosa_chinensis RchiOBHm_Chr6g0308471
rosa_laevigata RLG00000010640
rosa_multiflora Rmu_co8342223.1_g000001 Rmu_sc0001485.1_g000009 Rmu_sc0011796.1_g000010
rosa_roxburghii Rroxscaffold_7G00160100
rosa_rugosa Rorug06G0367000
rosa_samantha Rh6AG476800 Rh6BG487000 Rh6CG492100 Rh6DG478200
rosa_wichuraiana Rw6G041590

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 3 cut(s) 146, 215, 1309
AccBSI CCGCTC 3 cut(s) 1074, 1267, 1345
AccII CGCG 3 cut(s) 275, 923, 1094
AccIII TCCGGA 1 cut(s) 605
AcuI CTGAAG 1 cut(s) 57
AcyI GRCGYC 5 cut(s) 147, 204, 216, 882, 1136
AfaI GTAC 5 cut(s) 224, 467, 524, 1112, 1358
AfiI CCNNNNNNNGG 5 cut(s) 291, 535, 675, 737, 1306
AgeI ACCGGT 2 cut(s) 546, 559
AgsI TTSAA 5 cut(s) 7, 124, 598, 1061, 1409
AhlI ACTAGT 1 cut(s) 229
AjnI CCWGG 1 cut(s) 682
AleI CACNNNNGTG 1 cut(s) 776
AluBI AGCT 6 cut(s) 304, 761, 1027, 1108, 1126, 1147
AluI AGCT 6 cut(s) 304, 761, 1027, 1108, 1126, 1147
Alw21I GWGCWC 1 cut(s) 783
Alw26I GTCTC 3 cut(s) 607, 721, 1288
Alw44I GTGCAC 1 cut(s) 779
Aor13HI TCCGGA 1 cut(s) 605
AoxI GGCC 3 cut(s) 68, 341, 685
ApaLI GTGCAC 1 cut(s) 779
ApeKI GCWGC 6 cut(s) 649, 1001, 1105, 1147, 1348, 1377
AsiGI ACCGGT 2 cut(s) 546, 559
Asp700I GAANNNNTTC 1 cut(s) 548
AspLEI GCGC 8 cut(s) 149, 218, 277, 626, 743, 1051, 1094, 1291
AspS9I GGNCC 7 cut(s) 341, 514, 557, 562, 974, 1120, 1161
AsuC2I CCSGG 3 cut(s) 220, 340, 1231
AsuHPI GGTGA 7 cut(s) 328, 349, 358, 469, 610, 937, 1051
AvaII GGWCC 6 cut(s) 514, 557, 562, 974, 1120, 1161
BaeGI GKGCMC 1 cut(s) 783
BanI GGYRCC 3 cut(s) 146, 215, 1309
BauI CACGAG 2 cut(s) 673, 772
BbsI GAAGAC 2 cut(s) 877, 1375
Bbv12I GWGCWC 1 cut(s) 783
BbvI GCAGC 6 cut(s) 636, 1013, 1117, 1134, 1335, 1389
BccI CCATC 2 cut(s) 94, 430
BceAI ACGGC 5 cut(s) 160, 826, 944, 1011, 1390
BcgI CGANNNNNNTGC 4 cut(s) 631, 665, 1291, 1325
BciT130I CCWGG 1 cut(s) 684
BciVI GTATCC 1 cut(s) 985
BcnI CCSGG 3 cut(s) 220, 340, 1231
BcoDI GTCTC 3 cut(s) 607, 721, 1288
BcuI ACTAGT 1 cut(s) 229
BfaI CTAG 3 cut(s) 66, 230, 1248
BfmI CTRYAG 1 cut(s) 320
BfoI RGCGCY 2 cut(s) 150, 219
BfuI GTATCC 1 cut(s) 985
BglI GCCNNNNNGGC 1 cut(s) 693
Bme1390I CCNGG 4 cut(s) 220, 340, 684, 1231
Bme18I GGWCC 6 cut(s) 514, 557, 562, 974, 1120, 1161
BmgT120I GGNCC 7 cut(s) 341, 514, 557, 562, 974, 1120, 1161
BmrFI CCNGG 4 cut(s) 220, 340, 684, 1231
BmsI GCATC 4 cut(s) 671, 1119, 1216, 1279
BoxI GACNNNNGTC 1 cut(s) 203
BpiI GAAGAC 2 cut(s) 877, 1375
BpmI CTGGAG 3 cut(s) 572, 1049, 1295
BpuMI CCSGG 3 cut(s) 220, 340, 1231
BsaHI GRCGYC 5 cut(s) 147, 204, 216, 882, 1136
BsaI GGTCTC 1 cut(s) 721
BsaJI CCNNGG 3 cut(s) 259, 682, 808
BsaWI WCCGGW 6 cut(s) 359, 479, 546, 559, 605, 720
BsaXI ACNNNNNCTCC 4 cut(s) 75, 105, 383, 413
Bsc4I CCNNNNNNNGG 5 cut(s) 291, 535, 675, 737, 1306
Bse118I RCCGGY 5 cut(s) 407, 516, 546, 559, 620
Bse1I ACTGG 6 cut(s) 181, 205, 247, 511, 577, 883
BseAI TCCGGA 1 cut(s) 605
BseBI CCWGG 1 cut(s) 684
BseDI CCNNGG 3 cut(s) 259, 682, 808
BseGI GGATG 1 cut(s) 665
BseLI CCNNNNNNNGG 5 cut(s) 291, 535, 675, 737, 1306
BseMII CTCAG 5 cut(s) 305, 319, 839, 1283, 1307
BseNI ACTGG 6 cut(s) 181, 205, 247, 511, 577, 883
BseRI GAGGAG 5 cut(s) 151, 421, 896, 1034, 1037
BseSI GKGCMC 1 cut(s) 783
BseXI GCAGC 6 cut(s) 636, 1013, 1117, 1134, 1335, 1389
BseYI CCCAGC 1 cut(s) 1150
BsgI GTGCAG 1 cut(s) 816
Bsh1236I CGCG 3 cut(s) 275, 923, 1094
BshFI GGCC 3 cut(s) 70, 343, 687
BshNI GGYRCC 3 cut(s) 146, 215, 1309
BshTI ACCGGT 2 cut(s) 546, 559
BsiHKAI GWGCWC 1 cut(s) 783
BslFI GGGAC 6 cut(s) 235, 548, 987, 1174, 1183, 1258
BslI CCNNNNNNNGG 5 cut(s) 291, 535, 675, 737, 1306
BsmAI GTCTC 3 cut(s) 607, 721, 1288
BsmFI GGGAC 6 cut(s) 235, 548, 987, 1174, 1183, 1258
BsmI GAATGC 1 cut(s) 719
BsnI GGCC 3 cut(s) 70, 343, 687
Bso31I GGTCTC 1 cut(s) 721
Bsp1286I GDGCHC 1 cut(s) 783
Bsp13I TCCGGA 1 cut(s) 605
Bsp143I GATC 2 cut(s) 894, 1203
BspANI GGCC 3 cut(s) 70, 343, 687
BspCNI CTCAG 5 cut(s) 306, 318, 838, 1284, 1308
BspEI TCCGGA 1 cut(s) 605
BspFNI CGCG 3 cut(s) 275, 923, 1094
BspMAI CTGCAG 1 cut(s) 324
BspQI GCTCTTC 2 cut(s) 389, 1064
BspT107I GGYRCC 3 cut(s) 146, 215, 1309
BspTNI GGTCTC 1 cut(s) 721
BsrBI CCGCTC 3 cut(s) 1074, 1267, 1345
BsrFI RCCGGY 5 cut(s) 407, 516, 546, 559, 620
BsrI ACTGG 6 cut(s) 181, 205, 247, 511, 577, 883
BssAI RCCGGY 5 cut(s) 407, 516, 546, 559, 620
BssECI CCNNGG 3 cut(s) 259, 682, 808
BssMI GATC 2 cut(s) 894, 1203
BssNI GRCGYC 5 cut(s) 147, 204, 216, 882, 1136
BssSI CACGAG 2 cut(s) 673, 772
Bst2BI CACGAG 2 cut(s) 673, 772
Bst2UI CCWGG 1 cut(s) 684
Bst4CI ACNGT 2 cut(s) 55, 1400
Bst6I CTCTTC 4 cut(s) 389, 1037, 1064, 1211
BstACI GRCGYC 5 cut(s) 147, 204, 216, 882, 1136
BstC8I GCNNGC 4 cut(s) 80, 902, 1096, 1134
BstDEI CTNAG 5 cut(s) 305, 314, 825, 1292, 1316
BstDSI CCRYGG 1 cut(s) 808
BstF5I GGATG 1 cut(s) 665
BstFNI CGCG 3 cut(s) 275, 923, 1094
BstH2I RGCGCY 2 cut(s) 150, 219
BstHHI GCGC 8 cut(s) 149, 218, 277, 626, 743, 1051, 1094, 1291
BstKTI GATC 2 cut(s) 897, 1206
BstMAI GTCTC 3 cut(s) 607, 721, 1288
BstMBI GATC 2 cut(s) 894, 1203
BstMWI GCNNNNNNNGC 7 cut(s) 116, 201, 210, 340, 693, 1114, 1123
BstNI CCWGG 1 cut(s) 684
BstPAI GACNNNNGTC 1 cut(s) 203
BstSCI CCNGG 4 cut(s) 218, 338, 682, 1229
BstSFI CTRYAG 1 cut(s) 320
BstSLI GKGCMC 1 cut(s) 783
BstUI CGCG 3 cut(s) 275, 923, 1094
BstV1I GCAGC 6 cut(s) 636, 1013, 1117, 1134, 1335, 1389
BstV2I GAAGAC 2 cut(s) 877, 1375
BsuI GTATCC 1 cut(s) 985
BsuRI GGCC 3 cut(s) 70, 343, 687
BtgI CCRYGG 1 cut(s) 808
BtgZI GCGATG 1 cut(s) 408
BtsCI GGATG 1 cut(s) 665
BtsI GCAGTG 1 cut(s) 799
BtsIMutI CAGTG 5 cut(s) 51, 504, 559, 799, 876
Cac8I GCNNGC 4 cut(s) 80, 902, 1096, 1134
CfoI GCGC 8 cut(s) 149, 218, 277, 626, 743, 1051, 1094, 1291
Cfr10I RCCGGY 5 cut(s) 407, 516, 546, 559, 620
Cfr13I GGNCC 7 cut(s) 341, 514, 557, 562, 974, 1120, 1161
CseI GACGC 5 cut(s) 110, 193, 871, 1125, 1369
Csp6I GTAC 5 cut(s) 223, 466, 523, 1111, 1357
CspAI ACCGGT 2 cut(s) 546, 559
CviAII CATG 1 cut(s) 749
CviQI GTAC 5 cut(s) 223, 466, 523, 1111, 1357
DdeI CTNAG 5 cut(s) 305, 314, 825, 1292, 1316
DinI GGCGCC 2 cut(s) 148, 217
DpnI GATC 2 cut(s) 896, 1205
DpnII GATC 2 cut(s) 894, 1203
Eam1104I CTCTTC 4 cut(s) 389, 1037, 1064, 1211
EarI CTCTTC 4 cut(s) 389, 1037, 1064, 1211
EciI GGCGGA 3 cut(s) 753, 1001, 1157
Eco147I AGGCCT 2 cut(s) 70, 687
Eco31I GGTCTC 1 cut(s) 721
Eco47I GGWCC 6 cut(s) 514, 557, 562, 974, 1120, 1161
Eco57I CTGAAG 1 cut(s) 57
EcoRII CCWGG 1 cut(s) 682
EgeI GGCGCC 2 cut(s) 148, 217
EheI GGCGCC 2 cut(s) 148, 217
FaeI CATG 1 cut(s) 752
FaiI YATR 3 cut(s) 242, 750, 1177
FaqI GGGAC 6 cut(s) 235, 548, 987, 1174, 1183, 1258
FatI CATG 1 cut(s) 748
FauI CCCGC 5 cut(s) 426, 573, 1067, 1087, 1125
FokI GGATG 1 cut(s) 652
FspBI CTAG 3 cut(s) 66, 230, 1248
GlaI GCGC 8 cut(s) 148, 217, 276, 625, 742, 1050, 1093, 1290
GsaI CCCAGC 1 cut(s) 1154
GsuI CTGGAG 3 cut(s) 572, 1049, 1295
HaeII RGCGCY 2 cut(s) 150, 219
HaeIII GGCC 3 cut(s) 70, 343, 687
HgaI GACGC 5 cut(s) 110, 193, 871, 1125, 1369
HhaI GCGC 8 cut(s) 149, 218, 277, 626, 743, 1051, 1094, 1291
Hin1I GRCGYC 5 cut(s) 147, 204, 216, 882, 1136
Hin1II CATG 1 cut(s) 752
Hin6I GCGC 8 cut(s) 147, 216, 275, 624, 741, 1049, 1092, 1289
HinP1I GCGC 8 cut(s) 147, 216, 275, 624, 741, 1049, 1092, 1289
HincII GTYRAC 1 cut(s) 822
HindII GTYRAC 1 cut(s) 822
HphI GGTGA 7 cut(s) 328, 349, 358, 469, 610, 937, 1051
Hpy166II GTNNAC 7 cut(s) 557, 582, 754, 781, 822, 1357, 1396
Hpy188I TCNGA 3 cut(s) 308, 449, 642
Hpy8I GTNNAC 7 cut(s) 557, 582, 754, 781, 822, 1357, 1396
Hpy99I CGWCG 3 cut(s) 104, 119, 1141
HpyCH4III ACNGT 2 cut(s) 55, 1400
HpyCH4V TGCA 5 cut(s) 322, 662, 717, 781, 797
HpyF10VI GCNNNNNNNGC 7 cut(s) 116, 201, 210, 340, 693, 1114, 1123
HpyF3I CTNAG 5 cut(s) 305, 314, 825, 1292, 1316
Hsp92I GRCGYC 5 cut(s) 147, 204, 216, 882, 1136
Hsp92II CATG 1 cut(s) 752
HspAI GCGC 8 cut(s) 147, 216, 275, 624, 741, 1049, 1092, 1289
KasI GGCGCC 2 cut(s) 146, 215
Kpn2I TCCGGA 1 cut(s) 605
Kzo9I GATC 2 cut(s) 894, 1203
LguI GCTCTTC 2 cut(s) 389, 1064
LmnI GCTCC 7 cut(s) 591, 766, 909, 1024, 1030, 1077, 1144
Lsp1109I GCAGC 6 cut(s) 636, 1013, 1117, 1134, 1335, 1389
LweI GCATC 4 cut(s) 671, 1119, 1216, 1279
MaeI CTAG 3 cut(s) 66, 230, 1248
MaeIII GTNAC 3 cut(s) 55, 634, 833
MalI GATC 2 cut(s) 896, 1205
MbiI CCGCTC 3 cut(s) 1074, 1267, 1345
MboI GATC 2 cut(s) 894, 1203
MboII GAAGA 9 cut(s) 23, 376, 861, 877, 940, 1054, 1081, 1228, 1375
MhlI GDGCHC 1 cut(s) 783
MluCI AATT 1 cut(s) 964
Mly113I GGCGCC 2 cut(s) 147, 216
MlyI GAGTC 9 cut(s) 283, 318, 325, 357, 637, 817, 841, 1109, 1286
MmeI TCCRAC 2 cut(s) 189, 665
MroI TCCGGA 1 cut(s) 605
MroXI GAANNNNTTC 1 cut(s) 548
MseI TTAA 1 cut(s) 963
MslI CAYNNNNRTG 2 cut(s) 776, 1401
MspA1I CMGCKG 5 cut(s) 195, 1004, 1108, 1126, 1315
MspR9I CCNGG 4 cut(s) 220, 340, 684, 1231
Mva1269I GAATGC 1 cut(s) 719
MvaI CCWGG 1 cut(s) 684
MvnI CGCG 3 cut(s) 275, 923, 1094
MwoI GCNNNNNNNGC 7 cut(s) 116, 201, 210, 340, 693, 1114, 1123
NarI GGCGCC 2 cut(s) 147, 216
NciI CCSGG 3 cut(s) 220, 340, 1231
NdeII GATC 2 cut(s) 894, 1203
NlaIII CATG 1 cut(s) 752
NmeAIII GCCGAG 2 cut(s) 240, 855
NmuCI GTSAC 2 cut(s) 634, 833
OliI CACNNNNGTG 1 cut(s) 776
PceI AGGCCT 2 cut(s) 70, 687
PciSI GCTCTTC 2 cut(s) 389, 1064
PctI GAATGC 1 cut(s) 719
PdmI GAANNNNTTC 1 cut(s) 548
PfeI GAWTC 2 cut(s) 1057, 1303
PinAI ACCGGT 2 cut(s) 546, 559
PleI GAGTC 9 cut(s) 283, 317, 324, 357, 637, 817, 840, 1108, 1285
PluTI GGCGCC 2 cut(s) 150, 219
PpsI GAGTC 9 cut(s) 283, 317, 324, 357, 637, 817, 840, 1108, 1285
PshAI GACNNNNGTC 1 cut(s) 203
Psp6I CCWGG 1 cut(s) 682
PspFI CCCAGC 1 cut(s) 1150
PspGI CCWGG 1 cut(s) 682
PspPI GGNCC 7 cut(s) 341, 514, 557, 562, 974, 1120, 1161
PstI CTGCAG 1 cut(s) 324
PvuII CAGCTG 2 cut(s) 1108, 1126
RsaI GTAC 5 cut(s) 224, 467, 524, 1112, 1358
RsaNI GTAC 5 cut(s) 223, 466, 523, 1111, 1357
RseI CAYNNNNRTG 2 cut(s) 776, 1401
SapI GCTCTTC 2 cut(s) 389, 1064
SaqAI TTAA 1 cut(s) 963
Sau3AI GATC 2 cut(s) 894, 1203
Sau96I GGNCC 7 cut(s) 341, 514, 557, 562, 974, 1120, 1161
SchI GAGTC 9 cut(s) 283, 318, 325, 357, 637, 817, 841, 1109, 1286
ScrFI CCNGG 4 cut(s) 220, 340, 684, 1231
SduI GDGCHC 1 cut(s) 783
SfaNI GCATC 4 cut(s) 671, 1119, 1216, 1279
SfcI CTRYAG 1 cut(s) 320
SfoI GGCGCC 2 cut(s) 148, 217
SgrAI CRCCGGYG 1 cut(s) 620
SinI GGWCC 6 cut(s) 514, 557, 562, 974, 1120, 1161
SmiMI CAYNNNNRTG 2 cut(s) 776, 1401
SpeI ACTAGT 1 cut(s) 229
Sse9I AATT 1 cut(s) 964
SseBI AGGCCT 2 cut(s) 70, 687
SspDI GGCGCC 2 cut(s) 146, 215
SspMI CTAG 3 cut(s) 66, 230, 1248
StuI AGGCCT 2 cut(s) 70, 687
StyD4I CCNGG 4 cut(s) 218, 338, 682, 1229
TaaI ACNGT 2 cut(s) 55, 1400
TaqI TCGA 4 cut(s) 390, 456, 1224, 1263
TasI AATT 1 cut(s) 964
TatI WGTACW 2 cut(s) 522, 1110
TfiI GAWTC 2 cut(s) 1057, 1303
Tru1I TTAA 1 cut(s) 963
Tru9I TTAA 1 cut(s) 963
TscAI CASTG 5 cut(s) 58, 511, 559, 799, 883
TseFI GTSAC 2 cut(s) 634, 833
TseI GCWGC 6 cut(s) 649, 1001, 1105, 1147, 1348, 1377
Tsp45I GTSAC 2 cut(s) 634, 833
TspDTI ATGAA 1 cut(s) 229
TspRI CASTG 5 cut(s) 58, 511, 559, 799, 883
VneI GTGCAC 1 cut(s) 779
VpaK11BI GGWCC 6 cut(s) 514, 557, 562, 974, 1120, 1161
XmnI GAANNNNTTC 1 cut(s) 548
XspI CTAG 3 cut(s) 66, 230, 1248
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.