Rroxscaffold_7G00160100
NAC Family

inactive receptor kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0011041
Physical Location & Seq
Forward (+)
3118549 .. 3121931
3383 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00160100.1

Sequence Viewer

Length: 2022 bp
ATGCTCATCAACAAACGGAAACTACCCTCATTTCCTCTGTTTCTACTATTCACCTCTGTTTTCCTCTCATTTTGCACCACCTTAGTTACTAGTTCTTCAGTGAACTCATCATCGCCTTCAAACCCCTCAGATGCTGCTGCGCTTCTTGATTTCAAAGCAAAAGCTGATTTGAGCAGCGCACTTCCCTTCTCAAACACCACCACCCTCCATTTCTGTCAATGGGCTGGGATCGAATGCGCGAAATCGAGAGTGGTCCGGTTAGTAATCCAAGACTTGGATCTCGGCGGTGTTTTCGCACCGGACACTCTCACCCGCCTGGACCAACTCCGAGTACTCAGTCTCCAGAATGACTCGCTCACCGGACCGGTTCCCGATCTCTCCGGACTCATCAACCTCAAAACCCTCTTCCTTGATCACAACTCATTCTCCGGTTCTCTTCCGCATTCCCTCTCCTCTCTCCATAGGCTTCGAACCCTTGATTTCTCTTATAACAACCTCACCGGTCCATTGCCCGAATGGCTGAGCGGTCTGAACCGGCTCTACTATCTTCGCCTCGAGTGGAACCGGTTCAACGGAACCGTCCCGCCTCTGAACCAGTCCTCTCTCCAAACCTTTAACGTCTCCGGAAACAACCTCACCGGCGTCGTACCGGTCACTCCGACTCTCCTCCGCTTCGGACCGTCATCGTTCTTGTGGAACCCTAGTCTTTGCGGCGAGATCATTCGCATAGAATGCCACCCAAACGCACCGTTTTTCCGTCCGGCGACTCCGTCTACAGTTCCGGCAGCTCCGTCGCCTAGTCGCGCACTCGGGCTGAGAACCGCTCAGGGCGTTGAGTTGACTCAGCCGTCTCACAAGAAGCGCAAGAGAACCGCCGTCCTCATCGGCTTCTCTTCCGCCGTGTTCGTCCTGATCTGCTCGATCCTCTGCTTCGTCCTGGCCGTCAAGAATCAGAGGAAGCACGTCAAAAAGACGGACTTGTCCTCGGACGACGCGGCGGCGGCGCAGGCGGCGGCGGCGGTGCAGATGGAGCAGGAGGAGCTGGAGCAGAAGGTGAAGAGAGTTCAGGGGATTCAGGTGGTGAAGAGCGGGAGCTTGTTGTTCTGTGCGGGCGAGGCGCAGGTGTACTCGCTGGACCAGCTGATGAGGGCGTCGGCGGAGCTTTTGGGAAGAGGGACGCTGGGGTCCACCTACAAGGCGGTGCTGGACAACCGTCTGATCGTCTGCGTTAAACGGCTGGATGCTAGTGTGTTGGAGGGTACGGGTAGGGAAGCCTTCGAGAGGCACTTGGAATCGGTGGGAGGGCTCCGGCATCCGAACCTGGTTCCGCTCCGAGCTTATTTTCAGGCCAAAGAAGAGAGGCTCCTCATCTACGATTACCAGCCCAATGGCAGCCTCTTCTCTCTCATTCATGGTTCAAAATCAACGAGGGCAAAGCCTCTGCACTGGACATCATGCTTGAAAATAGCAGAGGACGTAGCGCAAGGCCTCTCTTACATCCACCAAGCATGGAGGCTTGTCCACGGAAATCTCAAGTCCTCCAACGTGCTACTTGGCTCCGACTTTGAGGCATGTGTCACTGACTACTGCCTCTCCGTTCTCGCTACTAATCTTCCGCCATGGGAGGAGGAGAACCCTGATTCTGCTGCCTATAAAGCACCAGAGACTCGCCATTCAAATCACTCACCGACTCCCAAGTCTGATGTGTATGCATATGGTATTTTGCTGGTGGAGCTTCTCACGGGGAGGCCTCCGTCGCAGCACTTAGTTTCGGTTCCCCTGAATGACATGATGCAATGGGTGAGGTCCGTGAGGGAAGAATCAGATGATGGTGGTGGTGGGGATTCACAAGAGACTAGTGACAAGATGGGAATGCTGCTGGACGTGGCCATAACTTGTAGCTCAGCTTCGCCGGAGCAGAGACCGACTATGTGGCAGGTTTTGAAGATGTTGCAGGAGATTAAACAGACTGTGGCCGCGGATGAAACTGAGACCGAGTTAATGGGCTTATGTCCTGGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

673

Amino Acids

73.77

Weight (kDa)

7.23

Isoelectric Point (pI)

46.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 103 - 189 2.1e-06 Leucine-rich repeat region
LRR_8 PF13855 131 - 190 2.2e-09 Leucine rich repeat
Pkinase PF00069 387 - 649 7.1e-32 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 387 - 650 7.1e-29 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011041)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G43020
fragaria_vesca FvH4_2g33931 FvH4_2g33931
malus_domestica MD08G1095100.v1.1 MD15G1078000.v1.1
prunus_persica Prupe.1G431300_v2.0.a1
pyrus_communis pycom08g07710 pycom15g07280
rosa_chinensis RchiOBHm_Chr6g0308471
rosa_laevigata RLG00000010640
rosa_multiflora Rmu_co8342223.1_g000001 Rmu_sc0001485.1_g000009 Rmu_sc0011796.1_g000010
rosa_roxburghii Rroxscaffold_7G00160100
rosa_rugosa Rorug06G0367000
rosa_samantha Rh6AG476800 Rh6BG487000 Rh6CG492100 Rh6DG478200
rosa_wichuraiana Rw6G041590

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 489
AarI CACCTGC 1 cut(s) 1111
AasI GACNNNNNNGTC 4 cut(s) 847, 979, 1183, 1696
Acc36I ACCTGC 2 cut(s) 1111, 1927
AccB7I CCANNNNNTGG 1 cut(s) 274
AccBSI CCGCTC 4 cut(s) 525, 824, 1089, 1330
AccI GTMKAC 1 cut(s) 773
AccII CGCG 4 cut(s) 239, 804, 995, 1979
AccIII TCCGGA 2 cut(s) 380, 623
AclWI GGATC 3 cut(s) 236, 285, 916
AcoI YGGCCR 3 cut(s) 939, 1887, 1974
AcuI CTGAAG 1 cut(s) 81
AcyI GRCGYC 2 cut(s) 642, 1151
AfaI GTAC 4 cut(s) 333, 648, 1127, 1261
AfiI CCNNNNNNNGG 2 cut(s) 274, 1281
AgeI ACCGGT 4 cut(s) 364, 500, 564, 649
AgsI TTSAA 7 cut(s) 120, 154, 571, 1419, 1462, 1677, 1945
AhlI ACTAGT 2 cut(s) 89, 1856
AjiI CACGTC 2 cut(s) 964, 1885
AjnI CCWGG 4 cut(s) 315, 936, 1320, 2014
Alw26I GTCTC 7 cut(s) 344, 625, 855, 1658, 1847, 1915, 1985
AlwI GGATC 3 cut(s) 236, 285, 916
AlwNI CAGNNNCTG 1 cut(s) 134
Ama87I CYCGRG 2 cut(s) 554, 809
Aor13HI TCCGGA 2 cut(s) 380, 623
AoxI GGCC 6 cut(s) 939, 1347, 1486, 1748, 1887, 1974
ApeKI GCWGC 8 cut(s) 134, 137, 174, 785, 1392, 1646, 1759, 1876
AsiGI ACCGGT 4 cut(s) 364, 500, 564, 649
Asp700I GAANNNNTTC 1 cut(s) 566
AspLEI GCGC 8 cut(s) 142, 179, 239, 806, 864, 1006, 1120, 1483
AspS9I GGNCC 8 cut(s) 253, 319, 362, 503, 677, 1135, 1185, 1806
AsuHPI GGTGA 9 cut(s) 43, 301, 349, 490, 628, 1066, 1093, 1677, 1813
AsuII TTCGAA 1 cut(s) 469
AvaI CYCGRG 2 cut(s) 554, 809
AvaII GGWCC 8 cut(s) 253, 319, 362, 503, 677, 1135, 1185, 1806
BalI TGGCCA 1 cut(s) 1889
BanII GRGCYC 1 cut(s) 1308
BarI GAAGNNNNNNTAC 2 cut(s) 1891, 1923
BbvI GCAGC 8 cut(s) 121, 124, 186, 797, 1404, 1633, 1771, 1863
BccI CCATC 3 cut(s) 1021, 1823, 1861
BceAI ACGGC 5 cut(s) 832, 860, 884, 926, 1250
BcgI CGANNNNNNTGC 2 cut(s) 774, 808
BciT130I CCWGG 4 cut(s) 317, 938, 1322, 2016
BclI TGATCA 1 cut(s) 412
BcoDI GTCTC 7 cut(s) 344, 625, 855, 1658, 1847, 1915, 1985
BcuI ACTAGT 2 cut(s) 89, 1856
BfaI CTAG 6 cut(s) 90, 702, 798, 1245, 1857, 2020
BfmI CTRYAG 1 cut(s) 774
BfuAI ACCTGC 2 cut(s) 1111, 1927
BglI GCCNNNNNGGC 1 cut(s) 517
BlpI GCTNAGC 2 cut(s) 521, 1903
BmcAI AGTACT 1 cut(s) 333
Bme1390I CCNGG 4 cut(s) 317, 938, 1322, 2016
Bme18I GGWCC 8 cut(s) 253, 319, 362, 503, 677, 1135, 1185, 1806
BmeT110I CYCGRG 2 cut(s) 554, 809
BmgBI CACGTC 2 cut(s) 964, 1885
BmgT120I GGNCC 8 cut(s) 253, 319, 362, 503, 677, 1135, 1185, 1806
BmrFI CCNGG 4 cut(s) 317, 938, 1322, 2016
BmsI GCATC 4 cut(s) 121, 1231, 1321, 1782
BoxI GACNNNNGTC 1 cut(s) 1212
BplI GAGNNNNNCTC 2 cut(s) 808, 840
BpmI CTGGAG 2 cut(s) 326, 1064
Bpu10I CCTNAGC 1 cut(s) 825
Bpu1102I GCTNAGC 2 cut(s) 521, 1903
Bpu14I TTCGAA 1 cut(s) 469
BpuEI CTTGAG 1 cut(s) 1517
BsaHI GRCGYC 2 cut(s) 642, 1151
BsaI GGTCTC 2 cut(s) 1915, 1985
BsaJI CCNNGG 4 cut(s) 984, 1522, 1619, 1977
BsaXI ACNNNNNCTCC 6 cut(s) 324, 354, 410, 440, 1577, 1607
Bsc4I CCNNNNNNNGG 2 cut(s) 274, 1281
Bse118I RCCGGY 6 cut(s) 364, 500, 534, 564, 638, 649
Bse1I ACTGG 2 cut(s) 595, 1451
Bse3DI GCAATG 2 cut(s) 506, 1802
BseAI TCCGGA 2 cut(s) 380, 623
BseBI CCWGG 4 cut(s) 317, 938, 1322, 2016
BseDI CCNNGG 4 cut(s) 984, 1522, 1619, 1977
BseGI GGATG 4 cut(s) 1246, 1312, 1497, 1987
BseLI CCNNNNNNNGG 2 cut(s) 274, 1281
BseMI GCAATG 2 cut(s) 506, 1802
BseMII CTCAG 8 cut(s) 141, 349, 512, 806, 839, 857, 1917, 1980
BseNI ACTGG 2 cut(s) 595, 1451
BseRI GAGGAG 6 cut(s) 442, 656, 1052, 1355, 1640, 1643
BseXI GCAGC 8 cut(s) 121, 124, 186, 797, 1404, 1633, 1771, 1863
BseYI CCCAGC 2 cut(s) 224, 1180
BsgI GTGCAG 2 cut(s) 1043, 1427
Bsh1236I CGCG 4 cut(s) 239, 804, 995, 1979
BshFI GGCC 6 cut(s) 941, 1349, 1488, 1750, 1889, 1976
BshTI ACCGGT 4 cut(s) 364, 500, 564, 649
BsiHKCI CYCGRG 2 cut(s) 554, 809
BslFI GGGAC 2 cut(s) 566, 1189
BslI CCNNNNNNNGG 2 cut(s) 274, 1281
BsmAI GTCTC 7 cut(s) 344, 625, 855, 1658, 1847, 1915, 1985
BsmBI CGTCTC 2 cut(s) 625, 855
BsmFI GGGAC 2 cut(s) 566, 1189
BsmI GAATGC 4 cut(s) 239, 442, 737, 1878
BsnI GGCC 6 cut(s) 941, 1349, 1488, 1750, 1889, 1976
Bso31I GGTCTC 2 cut(s) 1915, 1985
BsoBI CYCGRG 2 cut(s) 554, 809
Bsp119I TTCGAA 1 cut(s) 469
Bsp1286I GDGCHC 1 cut(s) 1308
Bsp13I TCCGGA 2 cut(s) 380, 623
Bsp143I GATC 8 cut(s) 228, 277, 373, 412, 717, 912, 921, 1218
Bsp1720I GCTNAGC 2 cut(s) 521, 1903
Bsp19I CCATGG 1 cut(s) 1619
BspANI GGCC 6 cut(s) 941, 1349, 1488, 1750, 1889, 1976
BspCNI CTCAG 8 cut(s) 140, 348, 513, 807, 838, 856, 1916, 1981
BspEI TCCGGA 2 cut(s) 380, 623
BspFNI CGCG 4 cut(s) 239, 804, 995, 1979
BspMI ACCTGC 2 cut(s) 1111, 1927
BspPI GGATC 3 cut(s) 236, 285, 916
BspQI GCTCTTC 1 cut(s) 1079
BspT104I TTCGAA 1 cut(s) 469
BspTNI GGTCTC 2 cut(s) 1915, 1985
BsrBI CCGCTC 4 cut(s) 525, 824, 1089, 1330
BsrDI GCAATG 2 cut(s) 506, 1802
BsrFI RCCGGY 6 cut(s) 364, 500, 534, 564, 638, 649
BsrI ACTGG 2 cut(s) 595, 1451
BssAI RCCGGY 6 cut(s) 364, 500, 534, 564, 638, 649
BssECI CCNNGG 4 cut(s) 984, 1522, 1619, 1977
BssMI GATC 8 cut(s) 228, 277, 373, 412, 717, 912, 921, 1218
BssNI GRCGYC 2 cut(s) 642, 1151
BssT1I CCWWGG 1 cut(s) 1619
Bst2UI CCWGG 4 cut(s) 317, 938, 1322, 2016
Bst4CI ACNGT 6 cut(s) 580, 681, 750, 778, 1214, 1972
Bst6I CTCTTC 8 cut(s) 410, 441, 898, 1052, 1079, 1165, 1350, 1403
BstACI GRCGYC 2 cut(s) 642, 1151
BstAPI GCANNNNNTGC 1 cut(s) 732
BstBI TTCGAA 1 cut(s) 469
BstC8I GCNNGC 2 cut(s) 1008, 1111
BstDSI CCRYGG 3 cut(s) 1522, 1619, 1977
BstENI CCTNNNNNAGG 1 cut(s) 1279
BstF5I GGATG 4 cut(s) 1246, 1312, 1497, 1987
BstFNI CGCG 4 cut(s) 239, 804, 995, 1979
BstHHI GCGC 8 cut(s) 142, 179, 239, 806, 864, 1006, 1120, 1483
BstKTI GATC 8 cut(s) 231, 280, 376, 415, 720, 915, 924, 1221
BstMAI GTCTC 7 cut(s) 344, 625, 855, 1658, 1847, 1915, 1985
BstMBI GATC 8 cut(s) 228, 277, 373, 412, 717, 912, 921, 1218
BstNI CCWGG 4 cut(s) 317, 938, 1322, 2016
BstNSI RCATGY 1 cut(s) 1575
BstPAI GACNNNNGTC 1 cut(s) 1212
BstSCI CCNGG 4 cut(s) 315, 936, 1320, 2014
BstSFI CTRYAG 1 cut(s) 774
BstUI CGCG 4 cut(s) 239, 804, 995, 1979
BstV1I GCAGC 8 cut(s) 121, 124, 186, 797, 1404, 1633, 1771, 1863
BstX2I RGATCY 1 cut(s) 277
BstXI CCANNNNNNTGG 1 cut(s) 1388
BstYI RGATCY 1 cut(s) 277
BsuRI GGCC 6 cut(s) 941, 1349, 1488, 1750, 1889, 1976
BtgI CCRYGG 3 cut(s) 1522, 1619, 1977
BtgZI GCGATG 1 cut(s) 96
BtrI CACGTC 2 cut(s) 964, 1885
BtsCI GGATG 4 cut(s) 1246, 1312, 1497, 1987
BtsIMutI CAGTG 3 cut(s) 105, 1444, 1578
BveI ACCTGC 2 cut(s) 1111, 1927
Cac8I GCNNGC 2 cut(s) 1008, 1111
CaiI CAGNNNCTG 1 cut(s) 134
CfoI GCGC 8 cut(s) 142, 179, 239, 806, 864, 1006, 1120, 1483
Cfr10I RCCGGY 6 cut(s) 364, 500, 534, 564, 638, 649
Cfr13I GGNCC 8 cut(s) 253, 319, 362, 503, 677, 1135, 1185, 1806
Cfr42I CCGCGG 1 cut(s) 1980
CpoI CGGWCCG 2 cut(s) 362, 677
CseI GACGC 4 cut(s) 631, 1001, 1140, 1186
CsiI ACCWGGT 1 cut(s) 1320
Csp6I GTAC 4 cut(s) 332, 647, 1126, 1260
CspAI ACCGGT 4 cut(s) 364, 500, 564, 649
CspI CGGWCCG 2 cut(s) 362, 677
CviAII CATG 6 cut(s) 1412, 1455, 1509, 1572, 1620, 1789
CviQI GTAC 4 cut(s) 332, 647, 1126, 1260
DpnI GATC 8 cut(s) 230, 279, 375, 414, 719, 914, 923, 1220
DpnII GATC 8 cut(s) 228, 277, 373, 412, 717, 912, 921, 1218
DrdI GACNNNNNNGTC 4 cut(s) 847, 979, 1183, 1696
DseDI GACNNNNNNGTC 4 cut(s) 847, 979, 1183, 1696
EaeI YGGCCR 3 cut(s) 939, 1887, 1974
Eam1104I CTCTTC 8 cut(s) 410, 441, 898, 1052, 1079, 1165, 1350, 1403
EarI CTCTTC 8 cut(s) 410, 441, 898, 1052, 1079, 1165, 1350, 1403
EciI GGCGGA 3 cut(s) 886, 1172, 1605
Eco130I CCWWGG 1 cut(s) 1619
Eco147I AGGCCT 2 cut(s) 1488, 1750
Eco24I GRGCYC 1 cut(s) 1308
Eco31I GGTCTC 2 cut(s) 1915, 1985
Eco47I GGWCC 8 cut(s) 253, 319, 362, 503, 677, 1135, 1185, 1806
Eco57I CTGAAG 1 cut(s) 81
Eco88I CYCGRG 2 cut(s) 554, 809
EcoNI CCTNNNNNAGG 1 cut(s) 1279
EcoRII CCWGG 4 cut(s) 315, 936, 1320, 2014
EcoT14I CCWWGG 1 cut(s) 1619
EcoT22I ATGCAT 1 cut(s) 1714
EcoT38I GRGCYC 1 cut(s) 1308
ErhI CCWWGG 1 cut(s) 1619
Esp3I CGTCTC 2 cut(s) 625, 855
FaeI CATG 6 cut(s) 1415, 1458, 1512, 1575, 1623, 1792
FalI AAGNNNNNCTT 2 cut(s) 962, 994
FaqI GGGAC 2 cut(s) 566, 1189
FatI CATG 6 cut(s) 1411, 1454, 1508, 1571, 1619, 1788
FauI CCCGC 4 cut(s) 320, 591, 1082, 1102
FauNDI CATATG 1 cut(s) 1714
FbaI TGATCA 1 cut(s) 412
FblI GTMKAC 1 cut(s) 773
FokI GGATG 4 cut(s) 1253, 1299, 1484, 1994
FriOI GRGCYC 1 cut(s) 1308
FspBI CTAG 6 cut(s) 90, 702, 798, 1245, 1857, 2020
GlaI GCGC 8 cut(s) 141, 178, 238, 805, 863, 1005, 1119, 1482
GsaI CCCAGC 2 cut(s) 228, 1184
GsuI CTGGAG 2 cut(s) 326, 1064
HaeIII GGCC 6 cut(s) 941, 1349, 1488, 1750, 1889, 1976
HgaI GACGC 4 cut(s) 631, 1001, 1140, 1186
HhaI GCGC 8 cut(s) 142, 179, 239, 806, 864, 1006, 1120, 1483
Hin1I GRCGYC 2 cut(s) 642, 1151
Hin1II CATG 6 cut(s) 1415, 1458, 1512, 1575, 1623, 1792
Hin6I GCGC 8 cut(s) 140, 177, 237, 804, 862, 1004, 1118, 1481
HinP1I GCGC 8 cut(s) 140, 177, 237, 804, 862, 1004, 1118, 1481
HincII GTYRAC 1 cut(s) 840
HindII GTYRAC 1 cut(s) 840
HphI GGTGA 9 cut(s) 43, 301, 349, 490, 628, 1066, 1093, 1677, 1813
Hpy166II GTNNAC 6 cut(s) 103, 774, 840, 1126, 1188, 1522
Hpy188III TCNNGA 9 cut(s) 146, 246, 343, 371, 381, 624, 910, 946, 1279
Hpy8I GTNNAC 6 cut(s) 103, 774, 840, 1126, 1188, 1522
Hpy99I CGWCG 5 cut(s) 647, 796, 995, 1156, 1759
HpyAV CCTTC 4 cut(s) 126, 196, 1045, 1285
HpyCH4III ACNGT 6 cut(s) 580, 681, 750, 778, 1214, 1972
HpyCH4IV ACGT 5 cut(s) 618, 963, 1476, 1545, 1884
HpyCH4V TGCA 6 cut(s) 75, 1024, 1444, 1712, 1795, 1954
HpySE526I ACGT 5 cut(s) 618, 963, 1476, 1545, 1884
Hsp92I GRCGYC 2 cut(s) 642, 1151
Hsp92II CATG 6 cut(s) 1415, 1458, 1512, 1575, 1623, 1792
HspAI GCGC 8 cut(s) 140, 177, 237, 804, 862, 1004, 1118, 1481
Kpn2I TCCGGA 2 cut(s) 380, 623
Ksp22I TGATCA 1 cut(s) 412
KspI CCGCGG 1 cut(s) 1980
Kzo9I GATC 8 cut(s) 228, 277, 373, 412, 717, 912, 921, 1218
LguI GCTCTTC 1 cut(s) 1079
Lsp1109I GCAGC 8 cut(s) 121, 124, 186, 797, 1404, 1633, 1771, 1863
LweI GCATC 4 cut(s) 121, 1231, 1321, 1782
MabI ACCWGGT 1 cut(s) 1320
MaeI CTAG 6 cut(s) 90, 702, 798, 1245, 1857, 2020
MaeII ACGT 5 cut(s) 618, 963, 1476, 1545, 1884
MaeIII GTNAC 4 cut(s) 85, 652, 1576, 1859
MalI GATC 8 cut(s) 230, 279, 375, 414, 719, 914, 923, 1220
MbiI CCGCTC 4 cut(s) 525, 824, 1089, 1330
MboI GATC 8 cut(s) 228, 277, 373, 412, 717, 912, 921, 1218
MflI RGATCY 1 cut(s) 277
MhlI GDGCHC 1 cut(s) 1308
MlsI TGGCCA 1 cut(s) 1889
MluNI TGGCCA 1 cut(s) 1889
MlyI GAGTC 7 cut(s) 344, 378, 655, 760, 835, 1660, 1684
MmeI TCCRAC 4 cut(s) 683, 1233, 1566, 1584
Mox20I TGGCCA 1 cut(s) 1889
Mph1103I ATGCAT 1 cut(s) 1714
MroI TCCGGA 2 cut(s) 380, 623
MroXI GAANNNNTTC 1 cut(s) 566
MscI TGGCCA 1 cut(s) 1889
MseI TTAA 4 cut(s) 615, 1230, 1962, 2000
Msp20I TGGCCA 1 cut(s) 1889
MspA1I CMGCKG 2 cut(s) 1141, 1979
MspR9I CCNGG 4 cut(s) 317, 938, 1322, 2016
Mva1269I GAATGC 4 cut(s) 239, 442, 737, 1878
MvaI CCWGG 4 cut(s) 317, 938, 1322, 2016
MvnI CGCG 4 cut(s) 239, 804, 995, 1979
NcoI CCATGG 1 cut(s) 1619
NdeI CATATG 1 cut(s) 1714
NdeII GATC 8 cut(s) 228, 277, 373, 412, 717, 912, 921, 1218
NlaIII CATG 6 cut(s) 1415, 1458, 1512, 1575, 1623, 1792
NmeAIII GCCGAG 1 cut(s) 261
NmuCI GTSAC 3 cut(s) 652, 1576, 1859
NsiI ATGCAT 1 cut(s) 1714
NspI RCATGY 1 cut(s) 1575
NspV TTCGAA 1 cut(s) 469
PaeR7I CTCGAG 1 cut(s) 554
PaqCI CACCTGC 1 cut(s) 1111
PceI AGGCCT 2 cut(s) 1488, 1750
PciSI GCTCTTC 1 cut(s) 1079
PcsI WCGNNNNNNNCGW 1 cut(s) 939
PctI GAATGC 4 cut(s) 239, 442, 737, 1878
PdmI GAANNNNTTC 1 cut(s) 566
PfeI GAWTC 6 cut(s) 949, 1072, 1292, 1640, 1820, 1844
PflFI GACNNNGTC 1 cut(s) 769
PflMI CCANNNNNTGG 1 cut(s) 274
PinAI ACCGGT 4 cut(s) 364, 500, 564, 649
PleI GAGTC 7 cut(s) 344, 378, 655, 760, 835, 1660, 1684
PpsI GAGTC 7 cut(s) 344, 378, 655, 760, 835, 1660, 1684
PshAI GACNNNNGTC 1 cut(s) 1212
PsiI TTATAA 1 cut(s) 489
Psp6I CCWGG 4 cut(s) 315, 936, 1320, 2014
PspFI CCCAGC 2 cut(s) 224, 1180
PspGI CCWGG 4 cut(s) 315, 936, 1320, 2014
PspPI GGNCC 8 cut(s) 253, 319, 362, 503, 677, 1135, 1185, 1806
PspXI VCTCGAGB 1 cut(s) 554
PsrI GAACNNNNNNTAC 2 cut(s) 524, 556
PstNI CAGNNNCTG 1 cut(s) 134
PsuI RGATCY 1 cut(s) 277
PsyI GACNNNGTC 1 cut(s) 769
PvuII CAGCTG 1 cut(s) 1141
RsaI GTAC 4 cut(s) 333, 648, 1127, 1261
RsaNI GTAC 4 cut(s) 332, 647, 1126, 1260
Rsr2I CGGWCCG 2 cut(s) 362, 677
RsrII CGGWCCG 2 cut(s) 362, 677
SacII CCGCGG 1 cut(s) 1980
SapI GCTCTTC 1 cut(s) 1079
SaqAI TTAA 4 cut(s) 615, 1230, 1962, 2000
Sau3AI GATC 8 cut(s) 228, 277, 373, 412, 717, 912, 921, 1218
Sau96I GGNCC 8 cut(s) 253, 319, 362, 503, 677, 1135, 1185, 1806
ScaI AGTACT 1 cut(s) 333
SchI GAGTC 7 cut(s) 344, 378, 655, 760, 835, 1660, 1684
ScrFI CCNGG 4 cut(s) 317, 938, 1322, 2016
SduI GDGCHC 1 cut(s) 1308
SexAI ACCWGGT 1 cut(s) 1320
SfaNI GCATC 4 cut(s) 121, 1231, 1321, 1782
SfcI CTRYAG 1 cut(s) 774
Sfr274I CTCGAG 1 cut(s) 554
Sfr303I CCGCGG 1 cut(s) 1980
SfuI TTCGAA 1 cut(s) 469
SgrAI CRCCGGYG 1 cut(s) 638
SgrBI CCGCGG 1 cut(s) 1980
SinI GGWCC 8 cut(s) 253, 319, 362, 503, 677, 1135, 1185, 1806
SlaI CTCGAG 1 cut(s) 554
SmlI CTYRAG 2 cut(s) 554, 1532
SmoI CTYRAG 2 cut(s) 554, 1532
SpeI ACTAGT 2 cut(s) 89, 1856
SseBI AGGCCT 2 cut(s) 1488, 1750
SspMI CTAG 6 cut(s) 90, 702, 798, 1245, 1857, 2020
StuI AGGCCT 2 cut(s) 1488, 1750
StyD4I CCNGG 4 cut(s) 315, 936, 1320, 2014
StyI CCWWGG 1 cut(s) 1619
TaaI ACNGT 6 cut(s) 580, 681, 750, 778, 1214, 1972
TaiI ACGT 5 cut(s) 621, 966, 1479, 1548, 1887
TaqI TCGA 6 cut(s) 231, 245, 469, 555, 920, 1278
TaqII GACCGA 2 cut(s) 1939, 2009
TatI WGTACW 2 cut(s) 331, 1125
TauI GCSGC 8 cut(s) 714, 998, 1001, 1004, 1013, 1016, 1019, 1979
TfiI GAWTC 6 cut(s) 949, 1072, 1292, 1640, 1820, 1844
Tru1I TTAA 4 cut(s) 615, 1230, 1962, 2000
Tru9I TTAA 4 cut(s) 615, 1230, 1962, 2000
TscAI CASTG 3 cut(s) 105, 1451, 1585
TseFI GTSAC 3 cut(s) 652, 1576, 1859
TseI GCWGC 8 cut(s) 134, 137, 174, 785, 1392, 1646, 1759, 1876
Tsp45I GTSAC 3 cut(s) 652, 1576, 1859
TspDTI ATGAA 2 cut(s) 1400, 1998
TspRI CASTG 3 cut(s) 105, 1451, 1585
Tth111I GACNNNGTC 1 cut(s) 769
Van91I CCANNNNNTGG 1 cut(s) 274
VpaK11BI GGWCC 8 cut(s) 253, 319, 362, 503, 677, 1135, 1185, 1806
XagI CCTNNNNNAGG 1 cut(s) 1279
XceI RCATGY 1 cut(s) 1575
XcmI CCANNNNNNNNNTGG 1 cut(s) 513
XhoI CTCGAG 1 cut(s) 554
XmiI GTMKAC 1 cut(s) 773
XmnI GAANNNNTTC 1 cut(s) 566
XspI CTAG 6 cut(s) 90, 702, 798, 1245, 1857, 2020
ZrmI AGTACT 1 cut(s) 333
Zsp2I ATGCAT 1 cut(s) 1714
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.