MD15G1078000.v1.1
NAC Family

inactive receptor kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0011041
Physical Location & Seq
Reverse (-)
5321577 .. 5324640
3064 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1078000.v1.1.491

Sequence Viewer

Length: 2019 bp
ATGCTCAGGAAATGGCAGCTCCTTTTACTTCTTCTCTCACTCTGCACCTCCACACTTACTTCCTCTAGGCCTTCCCTCGCTCATCCATCTCTCCCACCCGACGCTCTCGCGCTCCTCGCCTTCAAATCCAAAGCGGATTTACATGACGCCCTCCCCTTCTCTTCCAACGCCACCGCCGTCCAGTCCATCTGCCGCTGGACTGGCGTCCAATGCGCCGCCCGGTACAAAATAGTTCGCCTCGTAATCAAGTCCCAGAACCTCGGCGGCATTTTCGCGCCTGACACCCTGACTCGGTTGGACCAACTCAGAGTCCTGAGTCTGCAGAACAACTCGCTTACCGGACCGGTTCCCGACCTCGCTGGATTCACTAACCTCAAAACCCTCTTCCTCGACCACAACTCCTTCTCGGGTTCCTTCCCGCCATCGCTCTCCTCCCTCTACCTCCTCCGAACCCTCGATTTGTCGTACAACAACCTCACCGGATCATTACCCGCATTTCTAATCACCGATCTGGACCGGCTGTACTACCTTCGTCTCGAATGGAACCGGTTCACTGGACCGGTCCCGGCGCTAAACCAGTCCAACCTCCAGACCTTCAATGTCTCCGGAAACAACCTCACCGGTGCAATCCCTGTCACTCCGACTCTGCTCCGGTTCGGTGCATCCTCCTTCTCTTGGAACCCCTTCCTCTGCGGCGAGATTGTGAACAAAGAATGCAACGATACGACACCCTTTTTTGGTACTACTGAAGCCCACGGAGCCCCTCCGCCAGCGAAGGCGCTCGGGCAGAGCAGTGCAGAAGATATCCAGGGCGTTGAGTTGACTCAGCCTAGTCACAAAAAGCACAGAAGAACTGCCGTCATTATTGGGTTTTCCTCCGGCGTCTTCTTCCTGATCTGCTCCCTTCTCTGTTTCGCAATGGCGGTGAAGAAACAAAGGACACCCCAAACCCGCAAAACAGTTAATTCGGCGGGACCAACCGTAACGGAGGAAACAGCGGCGGCGGTCGTGGAAATAGAAGAGGAGCTGGAGCAGAAGGTGAAGAAGGCGCAGGGGATTCAAGTGGTGAAGAGCGGGAGCCTCATGTTCTGCGCGGGAGAGCCGCAGCTGTACTCGCTGGACCAGCTGATGCGGGCTTCGGCGGAGCTGCTGGGGAAGGGGACGATCGGGAAGACGTACAAGGCGGTGCTGGACATCCGCCTGATCGCGAGCGTAAAGAGGCTCGATGCCGGGAAGCTGGGTGGGACTAGCAGAGAGGTGTTCGAGCGGCACCTGGAGTCGGTGGGTGCGCTGAGACACCCGAATCTCGTGCCGCTAAGAGCATATTTTCAGGCGAAGGACGAGCGGCTGCTCGTCTACGATTACCAGCCTAACGGCAGCGTCTTCTCCCTCGTTCACGGGAAATCAACAAGGGCAAGGCCGCTGCACTGGACATCCTGCTTGAAAATAGCAGAGGACGTAGCACAAGGCCTCTCCTACATCCACCAAGCATGGAAGCTCGTCCACGGCAATCTCAAGTCCTCCAACGTCCTTCTTGGCTCCAACTTCGAAGCATGTCTCACCGACTACTGCCTCTCCGTTCTCGCCACCCCTACCCCAACGTCCGAAGCGGACCCCGATTCAACTGCGTACAAAGCCCCGGAGACTCGCACCAACTCATCAAACGATCACGATCATTATCGACAACGGCGGCAACCAACTCCAAAGTCCGATGTCTATGCATTCGGGGTTTTGTTGGTGGAGCTTCTCACGGGGAAGCCTCCATCGCAACACTTAGTCTTAGCGCCGGATGATACGATGGAATGGGTGAGATCTTTGAGGGAAGATGATTGTGACGATGGGCATGATAAGATGGCAATGCTGCTGGACGTGGCGATCGCTTGCAGTTCAACTTCGCCGGAGCAGAGACCGACCATGTGGCAGGTGTTGAAGATGTTACAGGAGATTAAAGAGGAGATGATATCATCCATGGAGGAGGTTGAAAGTGAATTGAGACGAACACACATGCTAGCTAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

673

Amino Acids

74.06

Weight (kDa)

7.9

Isoelectric Point (pI)

43.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_4 PF12799 101 - 135 1e-05 Leucine Rich repeats (2 copies)
Pkinase PF00069 382 - 644 2.5e-28 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 382 - 645 1e-26 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011041)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G43020
fragaria_vesca FvH4_2g33931 FvH4_2g33931
malus_domestica MD08G1095100.v1.1 MD15G1078000.v1.1
prunus_persica Prupe.1G431300_v2.0.a1
pyrus_communis pycom08g07710 pycom15g07280
rosa_chinensis RchiOBHm_Chr6g0308471
rosa_laevigata RLG00000010640
rosa_multiflora Rmu_co8342223.1_g000001 Rmu_sc0001485.1_g000009 Rmu_sc0011796.1_g000010
rosa_roxburghii Rroxscaffold_7G00160100
rosa_rugosa Rorug06G0367000
rosa_samantha Rh6AG476800 Rh6BG487000 Rh6CG492100 Rh6DG478200
rosa_wichuraiana Rw6G041590

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1912
Acc36I ACCTGC 1 cut(s) 1912
AccB1I GGYRCC 1 cut(s) 1269
AccBSI CCGCTC 3 cut(s) 1074, 1267, 1345
AccI GTMKAC 1 cut(s) 1356
AccII CGCG 4 cut(s) 110, 275, 1094, 1208
AccIII TCCGGA 1 cut(s) 605
AclWI GGATC 1 cut(s) 490
AcuI CTGAAG 1 cut(s) 768
AcyI GRCGYC 3 cut(s) 147, 204, 882
AfaI GTAC 7 cut(s) 224, 467, 524, 742, 1112, 1178, 1631
AfiI CCNNNNNNNGG 4 cut(s) 291, 675, 737, 1279
AgeI ACCGGT 4 cut(s) 343, 546, 559, 620
AgsI TTSAA 8 cut(s) 124, 598, 1061, 1444, 1623, 1890, 1930, 1982
AjiI CACGTC 1 cut(s) 1870
AjnI CCWGG 2 cut(s) 807, 1272
Alw26I GTCTC 7 cut(s) 539, 607, 1288, 1562, 1637, 1900, 1987
AlwI GGATC 1 cut(s) 490
Ama87I CYCGRG 2 cut(s) 406, 782
Aor13HI TCCGGA 1 cut(s) 605
AoxI GGCC 3 cut(s) 68, 1418, 1468
ApeKI GCWGC 7 cut(s) 16, 1105, 1147, 1348, 1377, 1423, 1861
AsiGI ACCGGT 4 cut(s) 343, 546, 559, 620
AsiSI GCGATCGC 1 cut(s) 1878
Asp700I GAANNNNTTC 2 cut(s) 548, 683
AspLEI GCGC 9 cut(s) 112, 215, 277, 571, 781, 1051, 1094, 1291, 1786
AspS9I GGNCC 8 cut(s) 298, 341, 514, 557, 562, 974, 1120, 1612
AsuC2I CCSGG 4 cut(s) 220, 566, 1231, 1640
AsuHPI GGTGA 8 cut(s) 469, 496, 610, 937, 1051, 1078, 1552, 1819
AsuII TTCGAA 1 cut(s) 1548
AsuNHI GCTAGC 2 cut(s) 2008, 2012
AvaI CYCGRG 2 cut(s) 406, 782
AvaII GGWCC 8 cut(s) 298, 341, 514, 557, 562, 974, 1120, 1612
BanI GGYRCC 1 cut(s) 1269
BanII GRGCYC 1 cut(s) 763
BauI CACGAG 1 cut(s) 1307
BbsI GAAGAC 3 cut(s) 877, 1178, 1375
BbvI GCAGC 7 cut(s) 28, 1117, 1134, 1335, 1389, 1410, 1848
BccI CCATC 7 cut(s) 94, 194, 430, 1771, 1792, 1832, 1846
BceAI ACGGC 5 cut(s) 161, 842, 1390, 1522, 1703
BcgI CGANNNNNNTGC 6 cut(s) 1291, 1325, 1607, 1641, 1700, 1734
BciT130I CCWGG 2 cut(s) 809, 1274
BcnI CCSGG 4 cut(s) 220, 566, 1231, 1640
BcoDI GTCTC 7 cut(s) 539, 607, 1288, 1562, 1637, 1900, 1987
BfaI CTAG 6 cut(s) 66, 831, 1248, 2009, 2013, 2017
BfmI CTRYAG 1 cut(s) 320
BfoI RGCGCY 3 cut(s) 572, 782, 1787
BfuAI ACCTGC 1 cut(s) 1912
BglII AGATCT 1 cut(s) 1811
Bme1390I CCNGG 6 cut(s) 220, 566, 809, 1231, 1274, 1640
Bme18I GGWCC 8 cut(s) 298, 341, 514, 557, 562, 974, 1120, 1612
BmeT110I CYCGRG 2 cut(s) 406, 782
BmgBI CACGTC 1 cut(s) 1870
BmgT120I GGNCC 8 cut(s) 298, 341, 514, 557, 562, 974, 1120, 1612
BmrFI CCNGG 6 cut(s) 220, 566, 809, 1231, 1274, 1640
BmsI GCATC 3 cut(s) 671, 1119, 1216
BmtI GCTAGC 2 cut(s) 2012, 2016
BoxI GACNNNNGTC 1 cut(s) 203
BpiI GAAGAC 3 cut(s) 877, 1178, 1375
BpmI CTGGAG 3 cut(s) 572, 1049, 1295
Bpu10I CCTNAGC 1 cut(s) 5
Bpu14I TTCGAA 1 cut(s) 1548
BpuEI CTTGAG 1 cut(s) 1499
BpuMI CCSGG 4 cut(s) 220, 566, 1231, 1640
BsaBI GATNNNNATC 2 cut(s) 1671, 1677
BsaHI GRCGYC 3 cut(s) 147, 204, 882
BsaI GGTCTC 1 cut(s) 1900
BsaJI CCNNGG 6 cut(s) 259, 754, 808, 1504, 1638, 1968
BsaWI WCCGGW 8 cut(s) 338, 343, 479, 546, 559, 605, 620, 651
BsaXI ACNNNNNCTCC 4 cut(s) 383, 413, 1559, 1589
Bsc4I CCNNNNNNNGG 4 cut(s) 291, 675, 737, 1279
Bse118I RCCGGY 5 cut(s) 343, 516, 546, 559, 620
Bse1I ACTGG 5 cut(s) 181, 205, 559, 577, 1433
Bse3DI GCAATG 2 cut(s) 924, 1863
Bse8I GATNNNNATC 2 cut(s) 1671, 1677
BseAI TCCGGA 1 cut(s) 605
BseBI CCWGG 2 cut(s) 809, 1274
BseDI CCNNGG 6 cut(s) 259, 754, 808, 1504, 1638, 1968
BseGI GGATG 7 cut(s) 82, 662, 1194, 1433, 1479, 1795, 1964
BseJI GATNNNNATC 2 cut(s) 1671, 1677
BseLI CCNNNNNNNGG 4 cut(s) 291, 675, 737, 1279
BseMI GCAATG 2 cut(s) 924, 1863
BseMII CTCAG 5 cut(s) 19, 305, 319, 839, 1283
BseNI ACTGG 5 cut(s) 181, 205, 559, 577, 1433
BseRI GAGGAG 6 cut(s) 104, 421, 434, 1037, 1967, 1988
BseXI GCAGC 7 cut(s) 28, 1117, 1134, 1335, 1389, 1410, 1848
BseYI CCCAGC 2 cut(s) 1150, 1237
BsgI GTGCAG 3 cut(s) 28, 816, 1409
Bsh1236I CGCG 4 cut(s) 110, 275, 1094, 1208
Bsh1285I CGRYCG 3 cut(s) 1008, 1167, 1878
BshFI GGCC 3 cut(s) 70, 1420, 1470
BshNI GGYRCC 1 cut(s) 1269
BshTI ACCGGT 4 cut(s) 343, 546, 559, 620
BsiEI CGRYCG 3 cut(s) 1008, 1167, 1878
BsiHKCI CYCGRG 2 cut(s) 406, 782
BslFI GGGAC 5 cut(s) 235, 548, 987, 1174, 1258
BslI CCNNNNNNNGG 4 cut(s) 291, 675, 737, 1279
BsmAI GTCTC 7 cut(s) 539, 607, 1288, 1562, 1637, 1900, 1987
BsmBI CGTCTC 2 cut(s) 539, 1987
BsmFI GGGAC 5 cut(s) 235, 548, 987, 1174, 1258
BsmI GAATGC 2 cut(s) 719, 1721
BsnI GGCC 3 cut(s) 70, 1420, 1470
Bso31I GGTCTC 1 cut(s) 1900
BsoBI CYCGRG 2 cut(s) 406, 782
Bsp119I TTCGAA 1 cut(s) 1548
Bsp1286I GDGCHC 1 cut(s) 763
Bsp13I TCCGGA 1 cut(s) 605
Bsp143I GATC 9 cut(s) 482, 508, 894, 1164, 1203, 1666, 1672, 1811, 1875
Bsp19I CCATGG 1 cut(s) 1968
Bsp68I TCGCGA 1 cut(s) 1208
BspANI GGCC 3 cut(s) 70, 1420, 1470
BspCNI CTCAG 5 cut(s) 18, 306, 318, 838, 1284
BspEI TCCGGA 1 cut(s) 605
BspFNI CGCG 4 cut(s) 110, 275, 1094, 1208
BspMAI CTGCAG 1 cut(s) 324
BspMI ACCTGC 1 cut(s) 1912
BspOI GCTAGC 2 cut(s) 2012, 2016
BspPI GGATC 1 cut(s) 490
BspQI GCTCTTC 1 cut(s) 1064
BspT104I TTCGAA 1 cut(s) 1548
BspT107I GGYRCC 1 cut(s) 1269
BspTNI GGTCTC 1 cut(s) 1900
BsrBI CCGCTC 3 cut(s) 1074, 1267, 1345
BsrDI GCAATG 2 cut(s) 924, 1863
BsrFI RCCGGY 5 cut(s) 343, 516, 546, 559, 620
BsrI ACTGG 5 cut(s) 181, 205, 559, 577, 1433
BssAI RCCGGY 5 cut(s) 343, 516, 546, 559, 620
BssECI CCNNGG 6 cut(s) 259, 754, 808, 1504, 1638, 1968
BssMI GATC 9 cut(s) 482, 508, 894, 1164, 1203, 1666, 1672, 1811, 1875
BssNI GRCGYC 3 cut(s) 147, 204, 882
BssSI CACGAG 1 cut(s) 1307
BssT1I CCWWGG 1 cut(s) 1968
Bst2BI CACGAG 1 cut(s) 1307
Bst2UI CCWGG 2 cut(s) 809, 1274
Bst4CI ACNGT 2 cut(s) 961, 982
Bst6I CTCTTC 4 cut(s) 166, 389, 1014, 1064
BstACI GRCGYC 3 cut(s) 147, 204, 882
BstBI TTCGAA 1 cut(s) 1548
BstC8I GCNNGC 6 cut(s) 771, 1134, 1210, 1882, 2010, 2014
BstDEI CTNAG 8 cut(s) 5, 305, 314, 825, 1292, 1316, 1774, 1780
BstDSI CCRYGG 3 cut(s) 754, 1504, 1968
BstF5I GGATG 7 cut(s) 82, 662, 1194, 1433, 1479, 1795, 1964
BstFNI CGCG 4 cut(s) 110, 275, 1094, 1208
BstH2I RGCGCY 3 cut(s) 572, 782, 1787
BstHHI GCGC 9 cut(s) 112, 215, 277, 571, 781, 1051, 1094, 1291, 1786
BstKTI GATC 9 cut(s) 485, 511, 897, 1167, 1206, 1669, 1675, 1814, 1878
BstMAI GTCTC 7 cut(s) 539, 607, 1288, 1562, 1637, 1900, 1987
BstMBI GATC 9 cut(s) 482, 508, 894, 1164, 1203, 1666, 1672, 1811, 1875
BstMCI CGRYCG 3 cut(s) 1008, 1167, 1878
BstMWI GCNNNNNNNGC 8 cut(s) 116, 201, 210, 758, 1114, 1123, 1634, 1765
BstNI CCWGG 2 cut(s) 809, 1274
BstNSI RCATGY 2 cut(s) 1557, 2008
BstPAI GACNNNNGTC 1 cut(s) 203
BstSCI CCNGG 6 cut(s) 218, 564, 807, 1229, 1272, 1638
BstSFI CTRYAG 1 cut(s) 320
BstUI CGCG 4 cut(s) 110, 275, 1094, 1208
BstV1I GCAGC 7 cut(s) 28, 1117, 1134, 1335, 1389, 1410, 1848
BstV2I GAAGAC 3 cut(s) 877, 1178, 1375
BstX2I RGATCY 1 cut(s) 1811
BstYI RGATCY 1 cut(s) 1811
BsuRI GGCC 3 cut(s) 70, 1420, 1470
BtgI CCRYGG 3 cut(s) 754, 1504, 1968
BtgZI GCGATG 2 cut(s) 408, 1749
BtrI CACGTC 1 cut(s) 1870
BtsCI GGATG 7 cut(s) 82, 662, 1194, 1433, 1479, 1795, 1964
BtsI GCAGTG 1 cut(s) 799
BtsIMutI CAGTG 3 cut(s) 552, 799, 1426
BtuMI TCGCGA 1 cut(s) 1208
BveI ACCTGC 1 cut(s) 1912
Cac8I GCNNGC 6 cut(s) 771, 1134, 1210, 1882, 2010, 2014
CfoI GCGC 9 cut(s) 112, 215, 277, 571, 781, 1051, 1094, 1291, 1786
Cfr10I RCCGGY 5 cut(s) 343, 516, 546, 559, 620
Cfr13I GGNCC 8 cut(s) 298, 341, 514, 557, 562, 974, 1120, 1612
CpoI CGGWCCG 1 cut(s) 341
CseI GACGC 5 cut(s) 110, 155, 193, 871, 1369
Csp6I GTAC 7 cut(s) 223, 466, 523, 741, 1111, 1177, 1630
CspAI ACCGGT 4 cut(s) 343, 546, 559, 620
CspI CGGWCCG 1 cut(s) 341
CviAII CATG 8 cut(s) 143, 1084, 1491, 1554, 1844, 1915, 1969, 2005
CviQI GTAC 7 cut(s) 223, 466, 523, 741, 1111, 1177, 1630
DdeI CTNAG 8 cut(s) 5, 305, 314, 825, 1292, 1316, 1774, 1780
DpnI GATC 9 cut(s) 484, 510, 896, 1166, 1205, 1668, 1674, 1813, 1877
DpnII GATC 9 cut(s) 482, 508, 894, 1164, 1203, 1666, 1672, 1811, 1875
Eam1104I CTCTTC 4 cut(s) 166, 389, 1014, 1064
EarI CTCTTC 4 cut(s) 166, 389, 1014, 1064
EciI GGCGGA 3 cut(s) 756, 1157, 1187
Eco130I CCWWGG 1 cut(s) 1968
Eco147I AGGCCT 2 cut(s) 70, 1470
Eco24I GRGCYC 1 cut(s) 763
Eco31I GGTCTC 1 cut(s) 1900
Eco32I GATATC 2 cut(s) 805, 1962
Eco47I GGWCC 8 cut(s) 298, 341, 514, 557, 562, 974, 1120, 1612
Eco57I CTGAAG 1 cut(s) 768
Eco88I CYCGRG 2 cut(s) 406, 782
EcoRII CCWGG 2 cut(s) 807, 1272
EcoRV GATATC 2 cut(s) 805, 1962
EcoT14I CCWWGG 1 cut(s) 1968
EcoT22I ATGCAT 1 cut(s) 1723
EcoT38I GRGCYC 1 cut(s) 763
ErhI CCWWGG 1 cut(s) 1968
Esp3I CGTCTC 2 cut(s) 539, 1987
FaeI CATG 8 cut(s) 146, 1087, 1494, 1557, 1847, 1918, 1972, 2008
FaqI GGGAC 5 cut(s) 235, 548, 987, 1174, 1258
FatI CATG 8 cut(s) 142, 1083, 1490, 1553, 1843, 1914, 1968, 2004
FauI CCCGC 7 cut(s) 426, 499, 959, 964, 1067, 1087, 1125
FblI GTMKAC 1 cut(s) 1356
FokI GGATG 7 cut(s) 69, 649, 1181, 1420, 1466, 1802, 1951
FriOI GRGCYC 1 cut(s) 763
FspBI CTAG 6 cut(s) 66, 831, 1248, 2009, 2013, 2017
GlaI GCGC 9 cut(s) 111, 214, 276, 570, 780, 1050, 1093, 1290, 1785
GsaI CCCAGC 2 cut(s) 1154, 1241
GsuI CTGGAG 3 cut(s) 572, 1049, 1295
HaeII RGCGCY 3 cut(s) 572, 782, 1787
HaeIII GGCC 3 cut(s) 70, 1420, 1470
HgaI GACGC 5 cut(s) 110, 155, 193, 871, 1369
HhaI GCGC 9 cut(s) 112, 215, 277, 571, 781, 1051, 1094, 1291, 1786
Hin1I GRCGYC 3 cut(s) 147, 204, 882
Hin1II CATG 8 cut(s) 146, 1087, 1494, 1557, 1847, 1918, 1972, 2008
Hin6I GCGC 9 cut(s) 110, 213, 275, 569, 779, 1049, 1092, 1289, 1784
HinP1I GCGC 9 cut(s) 110, 213, 275, 569, 779, 1049, 1092, 1289, 1784
HincII GTYRAC 1 cut(s) 822
HindII GTYRAC 1 cut(s) 822
HphI GGTGA 8 cut(s) 469, 496, 610, 937, 1051, 1078, 1552, 1819
Hpy166II GTNNAC 6 cut(s) 552, 706, 822, 1357, 1396, 1504
Hpy188I TCNGA 5 cut(s) 308, 449, 642, 1606, 1711
Hpy8I GTNNAC 6 cut(s) 552, 706, 822, 1357, 1396, 1504
Hpy99I CGWCG 1 cut(s) 104
HpyCH4III ACNGT 2 cut(s) 961, 982
HpyCH4IV ACGT 5 cut(s) 1175, 1458, 1527, 1601, 1869
HpyCH4V TGCA 9 cut(s) 45, 322, 626, 662, 717, 797, 1426, 1721, 1884
HpyF10VI GCNNNNNNNGC 8 cut(s) 116, 201, 210, 758, 1114, 1123, 1634, 1765
HpyF3I CTNAG 8 cut(s) 5, 305, 314, 825, 1292, 1316, 1774, 1780
HpySE526I ACGT 5 cut(s) 1175, 1458, 1527, 1601, 1869
Hsp92I GRCGYC 3 cut(s) 147, 204, 882
Hsp92II CATG 8 cut(s) 146, 1087, 1494, 1557, 1847, 1918, 1972, 2008
HspAI GCGC 9 cut(s) 110, 213, 275, 569, 779, 1049, 1092, 1289, 1784
Kpn2I TCCGGA 1 cut(s) 605
Kzo9I GATC 9 cut(s) 482, 508, 894, 1164, 1203, 1666, 1672, 1811, 1875
LguI GCTCTTC 1 cut(s) 1064
Lsp1109I GCAGC 7 cut(s) 28, 1117, 1134, 1335, 1389, 1410, 1848
LweI GCATC 3 cut(s) 671, 1119, 1216
MaeI CTAG 6 cut(s) 66, 831, 1248, 2009, 2013, 2017
MaeII ACGT 5 cut(s) 1175, 1458, 1527, 1601, 1869
MaeIII GTNAC 5 cut(s) 634, 833, 982, 1832, 1935
MalI GATC 9 cut(s) 484, 510, 896, 1166, 1205, 1668, 1674, 1813, 1877
MbiI CCGCTC 3 cut(s) 1074, 1267, 1345
MboI GATC 9 cut(s) 482, 508, 894, 1164, 1203, 1666, 1672, 1811, 1875
MflI RGATCY 1 cut(s) 1811
MhlI GDGCHC 1 cut(s) 763
MluCI AATT 2 cut(s) 964, 1988
MlyI GAGTC 7 cut(s) 283, 318, 325, 637, 817, 1286, 1639
MmeI TCCRAC 6 cut(s) 189, 276, 606, 665, 1548, 1566
Mph1103I ATGCAT 1 cut(s) 1723
MroI TCCGGA 1 cut(s) 605
MroXI GAANNNNTTC 2 cut(s) 548, 683
MseI TTAA 2 cut(s) 963, 1947
MspA1I CMGCKG 5 cut(s) 195, 998, 1108, 1126, 1423
MspR9I CCNGG 6 cut(s) 220, 566, 809, 1231, 1274, 1640
Mva1269I GAATGC 2 cut(s) 719, 1721
MvaI CCWGG 2 cut(s) 809, 1274
MvnI CGCG 4 cut(s) 110, 275, 1094, 1208
MwoI GCNNNNNNNGC 8 cut(s) 116, 201, 210, 758, 1114, 1123, 1634, 1765
NciI CCSGG 4 cut(s) 220, 566, 1231, 1640
NcoI CCATGG 1 cut(s) 1968
NdeII GATC 9 cut(s) 482, 508, 894, 1164, 1203, 1666, 1672, 1811, 1875
NheI GCTAGC 2 cut(s) 2008, 2012
NlaIII CATG 8 cut(s) 146, 1087, 1494, 1557, 1847, 1918, 1972, 2008
NmeAIII GCCGAG 1 cut(s) 240
NmuCI GTSAC 3 cut(s) 634, 833, 1832
NruI TCGCGA 1 cut(s) 1208
NsiI ATGCAT 1 cut(s) 1723
NspI RCATGY 2 cut(s) 1557, 2008
NspV TTCGAA 1 cut(s) 1548
PaqCI CACCTGC 1 cut(s) 1912
PceI AGGCCT 2 cut(s) 70, 1470
PciSI GCTCTTC 1 cut(s) 1064
PcsI WCGNNNNNNNCGW 1 cut(s) 174
PctI GAATGC 2 cut(s) 719, 1721
PdmI GAANNNNTTC 2 cut(s) 548, 683
PfeI GAWTC 4 cut(s) 363, 1057, 1303, 1619
PinAI ACCGGT 4 cut(s) 343, 546, 559, 620
Ple19I CGATCG 2 cut(s) 1167, 1878
PleI GAGTC 7 cut(s) 283, 317, 324, 637, 817, 1285, 1639
PpsI GAGTC 7 cut(s) 283, 317, 324, 637, 817, 1285, 1639
PshAI GACNNNNGTC 1 cut(s) 203
Psp6I CCWGG 2 cut(s) 807, 1272
PspFI CCCAGC 2 cut(s) 1150, 1237
PspGI CCWGG 2 cut(s) 807, 1272
PspPI GGNCC 8 cut(s) 298, 341, 514, 557, 562, 974, 1120, 1612
PstI CTGCAG 1 cut(s) 324
PsuI RGATCY 1 cut(s) 1811
PvuI CGATCG 2 cut(s) 1167, 1878
PvuII CAGCTG 2 cut(s) 1108, 1126
RgaI GCGATCGC 1 cut(s) 1878
RruI TCGCGA 1 cut(s) 1208
RsaI GTAC 7 cut(s) 224, 467, 524, 742, 1112, 1178, 1631
RsaNI GTAC 7 cut(s) 223, 466, 523, 741, 1111, 1177, 1630
Rsr2I CGGWCCG 1 cut(s) 341
RsrII CGGWCCG 1 cut(s) 341
SapI GCTCTTC 1 cut(s) 1064
SaqAI TTAA 2 cut(s) 963, 1947
Sau3AI GATC 9 cut(s) 482, 508, 894, 1164, 1203, 1666, 1672, 1811, 1875
Sau96I GGNCC 8 cut(s) 298, 341, 514, 557, 562, 974, 1120, 1612
SchI GAGTC 7 cut(s) 283, 318, 325, 637, 817, 1286, 1639
ScrFI CCNGG 6 cut(s) 220, 566, 809, 1231, 1274, 1640
SduI GDGCHC 1 cut(s) 763
SfaAI GCGATCGC 1 cut(s) 1878
SfaNI GCATC 3 cut(s) 671, 1119, 1216
SfcI CTRYAG 1 cut(s) 320
SfuI TTCGAA 1 cut(s) 1548
SgfI GCGATCGC 1 cut(s) 1878
SgrAI CRCCGGYG 1 cut(s) 620
SinI GGWCC 8 cut(s) 298, 341, 514, 557, 562, 974, 1120, 1612
SmlI CTYRAG 1 cut(s) 1514
SmoI CTYRAG 1 cut(s) 1514
Sse9I AATT 2 cut(s) 964, 1988
SseBI AGGCCT 2 cut(s) 70, 1470
SspMI CTAG 6 cut(s) 66, 831, 1248, 2009, 2013, 2017
StuI AGGCCT 2 cut(s) 70, 1470
StyD4I CCNGG 6 cut(s) 218, 564, 807, 1229, 1272, 1638
StyI CCWWGG 1 cut(s) 1968
TaaI ACNGT 2 cut(s) 961, 982
TaiI ACGT 5 cut(s) 1178, 1461, 1530, 1604, 1872
TaqI TCGA 7 cut(s) 390, 456, 537, 1224, 1263, 1548, 1681
TaqII GACCGA 1 cut(s) 1924
TasI AATT 2 cut(s) 964, 1988
TatI WGTACW 2 cut(s) 522, 1110
TfiI GAWTC 4 cut(s) 363, 1057, 1303, 1619
Tru1I TTAA 2 cut(s) 963, 1947
Tru9I TTAA 2 cut(s) 963, 1947
TscAI CASTG 3 cut(s) 559, 799, 1433
TseFI GTSAC 3 cut(s) 634, 833, 1832
TseI GCWGC 7 cut(s) 16, 1105, 1147, 1348, 1377, 1423, 1861
Tsp45I GTSAC 3 cut(s) 634, 833, 1832
TspGWI ACGGA 3 cut(s) 771, 1001, 1567
TspRI CASTG 3 cut(s) 559, 799, 1433
VpaK11BI GGWCC 8 cut(s) 298, 341, 514, 557, 562, 974, 1120, 1612
XceI RCATGY 2 cut(s) 1557, 2008
XmiI GTMKAC 1 cut(s) 1356
XmnI GAANNNNTTC 2 cut(s) 548, 683
XspI CTAG 6 cut(s) 66, 831, 1248, 2009, 2013, 2017
Zsp2I ATGCAT 1 cut(s) 1723
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.