MD08G1101000.v1.1

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0006308
Physical Location & Seq
Forward (+)
8495895 .. 8500908
5014 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1101000.v1.1.491

Sequence Viewer

Length: 1578 bp
ATGGACGGCGGCGATGGGACGATGAGGCTCGGCGCTCTCAACTTGAAGCCTGATCGGGTCGGCGGCTTCGAGTCGGGTCCCGACGTGTCGGTTTCGTCGCCGGTCACGCGGCAGAAGGCGGTGGCGGCAAAGCAGTTCATTGAGAATCACTACAAGAACTACCTTCAAGGTCTGCAGGAACGCAAAGAGAGACGTCGAGCGCTTCAAAAGAAAGCACAAGAAGATCAGATACCGAGTGAGGAACAAGAGGAGATGATGAGGACTTTGGAACGCCGGGAAACAGAATATATGAGGCTGCAAAGACGTAAAATCGGCATTGATGATTTTGAGCAATTAACTGTGATTGGCAAAGGCGCATTTGGTGAGGTAAGGTTATGTCGTGCTAAAGGTACAGGAGAGATTTATGCCATGAAGAAATTGAAGAAATCAGAGATGCTTAGTCGCGGACAGGTTGAGCATGTGCGTTCTGAGAGGAACTTGCTTGCAGAGGTTGATAGTCGGTTCATTGTACAACTTCATTATTCGTTTCAAGATTCCGATTTTTTATACCTTATCATGGAGTACTTACCTGGTGGCGATATTATGACATTACTGATGAGAGAAGATACTCTTTCTGAAGATGTTGCTCGTTTTTACATAGCAGAGAGTATTCTGGCTATTCACTCAATCCATCAACACAACTACATTCATAGGGACATAAAACCAGATAACCTGATACTGGATAAAACTGGCCATTTGAAGCTTTCAGATTTTGGCTTGTGTAAACCTCTGGATGACAAGTATTCCACATTGTTACTGGAAAATGATGATTTGACCTCCCAGGAATCCATATCTGAAACTGAAGAACAATCTGGTTGTGAAAAGGTTCCTTGGTTGATGCCAAAAGAACAGTTACAACAATGGAAACGAAATCGCCGTGCCTTGGCTTATTCAACCGTGGGAACTCTTGACTATATGGCACCTGAGGTGTTGCTCAAGAAAGGATATGGGATAGAGTGTGATTGGTGGTCCCTAGGGGCAATCATGTATGAGATGCTTGTAGGCTATGCTCCCTTCTGCTCTGATGACCCAAGGATTACATGCCGCAAGATAATCAATTGGAGGACATGCCTGAAATTCCCTGATGAACCCAAAATCTCGGAAGAAGCGAAGGATCTGATCTGTCACTTGTTATGTGATGTTGAGTCAAGGCTTGGGACACATGGAGCAGAAGAAATTAAGGCCCATCCATGGTTCAGGTCTACTCAGTGGGACAGGCTGTATGAAGGCGAAGCTGCATATAAACCCACAGTCGTTGGAGACTTGGACACCCAGAATTTCGAAAAGTTTCCTGAAGTAGAGGGACAGCAATCAACAATGCCAACAGTGGGACCTTGGCGGAAGATGTTAACATCAAAAGATACCAATTTCATTGGATACACTTTTAAGAAATCCGATGTCCTTAAATCGCTTGAAAGTTCAGGTACAGACATGCAATCAAATGGATCTTCAAAGTCCCCTTCTCTGATTTCCTTGTTAGGTAGAATTGACTTGCAAGAAACCATGATACCGGAGGGTGATCAGAAGCAGGATATATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000166 GO:0000902 GO:0000904 GO:0002009 GO:0002165 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005488 GO:0005524 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005938 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0007275 GO:0007399 GO:0007423 GO:0007444 GO:0007455 GO:0007469 GO:0007472 GO:0007476 GO:0007552 GO:0007560 GO:0008037 GO:0008038 GO:0008144 GO:0008150 GO:0008152 GO:0008544 GO:0009653 GO:0009791 GO:0009886 GO:0009887 GO:0009888 GO:0009913 GO:0009987 GO:0010769 GO:0010975 GO:0016043 GO:0016301 GO:0016310 GO:0016358 GO:0016740 GO:0016772 GO:0016773 GO:0017076 GO:0018105 GO:0018193 GO:0018209 GO:0019538 GO:0022008 GO:0022416 GO:0022603 GO:0022604 GO:0023052 GO:0030030 GO:0030154 GO:0030182 GO:0030424 GO:0030425 GO:0030554 GO:0030855 GO:0031175 GO:0031344 GO:0032501 GO:0032502 GO:0032553 GO:0032555 GO:0032559 GO:0032989 GO:0032990 GO:0035107 GO:0035114 GO:0035120 GO:0035214 GO:0035220 GO:0035239 GO:0035295 GO:0035315 GO:0035316 GO:0035317 GO:0035556 GO:0035639 GO:0036094 GO:0036211 GO:0036477 GO:0042995 GO:0043005 GO:0043167 GO:0043168 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044297 GO:0044424 GO:0044444 GO:0044463 GO:0044464 GO:0045595 GO:0045664 GO:0048468 GO:0048513 GO:0048563 GO:0048569 GO:0048666 GO:0048667 GO:0048699 GO:0048707 GO:0048729 GO:0048731 GO:0048736 GO:0048737 GO:0048800 GO:0048812 GO:0048813 GO:0048814 GO:0048856 GO:0048858 GO:0048869 GO:0050767 GO:0050773 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051128 GO:0051239 GO:0051716 GO:0051960 GO:0060284 GO:0060429 GO:0060562 GO:0065007 GO:0070451 GO:0070593 GO:0071704 GO:0071840 GO:0071944 GO:0097159 GO:0097367 GO:0097447 GO:0097458 GO:0099568 GO:0120025 GO:0120035 GO:0120036 GO:0120038 GO:0120039 GO:0140096 GO:1901265 GO:1901363 GO:1901564 GO:2000026
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase Pkinase_C

Protein Analysis

526

Amino Acids

60.23

Weight (kDa)

5.52

Isoelectric Point (pI)

50.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 109 - 262 7.8e-43 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 112 - 261 4.4e-21 Protein tyrosine and serine/threonine kinase
ABC1 PF03109 183 - 265 3.8e-07 ABC1 atypical kinase-like domain
Pkinase PF00069 309 - 412 1.7e-21 Protein kinase domain
Pkinase_C PF00433 432 - 475 3.5e-06 Protein kinase C terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 196
AccB1I GGYRCC 1 cut(s) 958
AccI GTMKAC 1 cut(s) 1241
AccII CGCG 2 cut(s) 109, 444
AciI CCGC 8 cut(s) 9, 63, 109, 119, 125, 444, 1084, 1378
AclWI GGATC 2 cut(s) 1161, 1492
AcoI YGGCCR 1 cut(s) 730
AcsI RAATTY 2 cut(s) 1115, 1315
AcuI CTGAAG 3 cut(s) 636, 861, 1353
AcyI GRCGYC 1 cut(s) 193
AfaI GTAC 4 cut(s) 391, 510, 563, 1465
AfeI AGCGCT 1 cut(s) 201
AfiI CCNNNNNNNGG 5 cut(s) 556, 718, 922, 1230, 1367
AflIII ACRYGT 1 cut(s) 84
AgsI TTSAA 9 cut(s) 46, 167, 206, 421, 530, 739, 933, 1454, 1491
AjiI CACGTC 1 cut(s) 85
AjnI CCWGG 2 cut(s) 568, 819
AluBI AGCT 2 cut(s) 742, 1274
AluI AGCT 2 cut(s) 742, 1274
Alw26I GTCTC 2 cut(s) 184, 1293
AlwI GGATC 2 cut(s) 1161, 1492
Aor51HI AGCGCT 1 cut(s) 201
AoxI GGCC 2 cut(s) 730, 1221
ApeKI GCWGC 2 cut(s) 295, 1274
ApoI RAATTY 2 cut(s) 1115, 1315
Asp700I GAANNNNTTC 2 cut(s) 864, 1326
AspA2I CCTAGG 1 cut(s) 1012
AspLEI GCGC 3 cut(s) 35, 202, 356
AspS9I GGNCC 4 cut(s) 77, 1008, 1222, 1370
AsuC2I CCSGG 1 cut(s) 275
AsuHPI GGTGA 2 cut(s) 374, 1568
AsuII TTCGAA 1 cut(s) 1320
AvaII GGWCC 3 cut(s) 77, 1008, 1370
AvrII CCTAGG 1 cut(s) 1012
AxyI CCTNAGG 1 cut(s) 963
BalI TGGCCA 1 cut(s) 732
BanI GGYRCC 1 cut(s) 958
BarI GAAGNNNNNNTAC 2 cut(s) 213, 245
BbvI GCAGC 2 cut(s) 282, 1261
BccI CCATC 3 cut(s) 8, 678, 1233
BceAI ACGGC 2 cut(s) 22, 900
BciT130I CCWGG 2 cut(s) 570, 821
BciVI GTATCC 1 cut(s) 1409
BclI TGATCA 1 cut(s) 1558
BcnI CCSGG 1 cut(s) 275
BcoDI GTCTC 2 cut(s) 184, 1293
BfaI CTAG 1 cut(s) 1013
BfmI CTRYAG 1 cut(s) 173
BfoI RGCGCY 2 cut(s) 36, 203
BfuI GTATCC 1 cut(s) 1409
BisI GCNGC 7 cut(s) 10, 64, 110, 126, 296, 1084, 1275
BlnI CCTAGG 1 cut(s) 1012
BlsI GCNGC 7 cut(s) 11, 65, 111, 127, 297, 1085, 1276
BmcAI AGTACT 1 cut(s) 563
Bme1390I CCNGG 3 cut(s) 275, 570, 821
Bme18I GGWCC 3 cut(s) 77, 1008, 1370
BmgBI CACGTC 1 cut(s) 85
BmgT120I GGNCC 4 cut(s) 77, 1008, 1222, 1370
BmiI GGNNCC 6 cut(s) 78, 79, 867, 960, 1010, 1371
BmrFI CCNGG 3 cut(s) 275, 570, 821
BmsI GCATC 3 cut(s) 423, 867, 1023
Bpu14I TTCGAA 1 cut(s) 1320
BpuEI CTTGAG 1 cut(s) 959
BpuMI CCSGG 1 cut(s) 275
BsaHI GRCGYC 1 cut(s) 193
BsaJI CCNNGG 8 cut(s) 819, 869, 921, 936, 1012, 1070, 1229, 1373
BsaWI WCCGGW 1 cut(s) 1549
Bsc4I CCNNNNNNNGG 5 cut(s) 556, 718, 922, 1230, 1367
Bse118I RCCGGY 1 cut(s) 100
Bse1I ACTGG 3 cut(s) 723, 733, 801
Bse21I CCTNAGG 1 cut(s) 963
BseBI CCWGG 2 cut(s) 570, 821
BseDI CCNNGG 8 cut(s) 819, 869, 921, 936, 1012, 1070, 1229, 1373
BseGI GGATG 2 cut(s) 778, 1225
BseLI CCNNNNNNNGG 5 cut(s) 556, 718, 922, 1230, 1367
BseMII CTCAG 3 cut(s) 459, 954, 1259
BseNI ACTGG 3 cut(s) 723, 733, 801
BseRI GAGGAG 1 cut(s) 263
BseXI GCAGC 2 cut(s) 282, 1261
Bsh1236I CGCG 2 cut(s) 109, 444
BshFI GGCC 2 cut(s) 732, 1223
BshNI GGYRCC 1 cut(s) 958
BsiSI CCGG 3 cut(s) 101, 274, 1550
BslFI GGGAC 9 cut(s) 31, 63, 707, 994, 1210, 1265, 1356, 1383, 1480
BslI CCNNNNNNNGG 5 cut(s) 556, 718, 922, 1230, 1367
BsmAI GTCTC 2 cut(s) 184, 1293
BsmBI CGTCTC 1 cut(s) 184
BsmFI GGGAC 9 cut(s) 31, 63, 707, 994, 1210, 1265, 1356, 1383, 1480
BsnI GGCC 2 cut(s) 732, 1223
Bsp119I TTCGAA 1 cut(s) 1320
Bsp1407I TGTACA 1 cut(s) 508
Bsp143I GATC 6 cut(s) 52, 223, 1153, 1158, 1484, 1558
Bsp19I CCATGG 1 cut(s) 1229
BspACI CCGC 8 cut(s) 9, 63, 109, 119, 125, 444, 1084, 1378
BspANI GGCC 2 cut(s) 732, 1223
BspCNI CTCAG 3 cut(s) 460, 955, 1258
BspFNI CGCG 2 cut(s) 109, 444
BspLI GGNNCC 6 cut(s) 78, 79, 867, 960, 1010, 1371
BspMAI CTGCAG 1 cut(s) 177
BspPI GGATC 2 cut(s) 1161, 1492
BspT104I TTCGAA 1 cut(s) 1320
BspT107I GGYRCC 1 cut(s) 958
BsrFI RCCGGY 1 cut(s) 100
BsrGI TGTACA 1 cut(s) 508
BsrI ACTGG 3 cut(s) 723, 733, 801
BssAI RCCGGY 1 cut(s) 100
BssECI CCNNGG 8 cut(s) 819, 869, 921, 936, 1012, 1070, 1229, 1373
BssMI GATC 6 cut(s) 52, 223, 1153, 1158, 1484, 1558
BssNI GRCGYC 1 cut(s) 193
BssT1I CCWWGG 6 cut(s) 869, 921, 1012, 1070, 1229, 1373
Bst2UI CCWGG 2 cut(s) 570, 821
Bst4CI ACNGT 5 cut(s) 340, 891, 937, 1291, 1366
BstACI GRCGYC 1 cut(s) 193
BstAUI TGTACA 1 cut(s) 508
BstBI TTCGAA 1 cut(s) 1320
BstC8I GCNNGC 1 cut(s) 483
BstDEI CTNAG 4 cut(s) 437, 468, 963, 1245
BstDSI CCRYGG 2 cut(s) 936, 1229
BstF5I GGATG 2 cut(s) 778, 1225
BstFNI CGCG 2 cut(s) 109, 444
BstH2I RGCGCY 2 cut(s) 36, 203
BstHHI GCGC 3 cut(s) 35, 202, 356
BstKTI GATC 6 cut(s) 55, 226, 1156, 1161, 1487, 1561
BstMAI GTCTC 2 cut(s) 184, 1293
BstMBI GATC 6 cut(s) 52, 223, 1153, 1158, 1484, 1558
BstMWI GCNNNNNNNGC 2 cut(s) 106, 125
BstNI CCWGG 2 cut(s) 570, 821
BstNSI RCATGY 4 cut(s) 461, 1083, 1110, 1474
BstSCI CCNGG 3 cut(s) 273, 568, 819
BstSFI CTRYAG 1 cut(s) 173
BstUI CGCG 2 cut(s) 109, 444
BstV1I GCAGC 2 cut(s) 282, 1261
BstX2I RGATCY 2 cut(s) 1153, 1484
BstYI RGATCY 2 cut(s) 1153, 1484
Bsu36I CCTNAGG 1 cut(s) 963
BsuI GTATCC 1 cut(s) 1409
BsuRI GGCC 2 cut(s) 732, 1223
BtgI CCRYGG 2 cut(s) 936, 1229
BtgZI GCGATG 1 cut(s) 27
BtrI CACGTC 1 cut(s) 85
BtsCI GGATG 2 cut(s) 778, 1225
BtsIMutI CAGTG 2 cut(s) 1253, 1371
Cac8I GCNNGC 1 cut(s) 483
CfoI GCGC 3 cut(s) 35, 202, 356
Cfr10I RCCGGY 1 cut(s) 100
Cfr13I GGNCC 4 cut(s) 77, 1008, 1222, 1370
CsiI ACCWGGT 1 cut(s) 568
Csp6I GTAC 4 cut(s) 390, 509, 562, 1464
CspCI CAANNNNNGTGG 2 cut(s) 1276, 1311
CviQI GTAC 4 cut(s) 390, 509, 562, 1464
DdeI CTNAG 4 cut(s) 437, 468, 963, 1245
DpnI GATC 6 cut(s) 54, 225, 1155, 1160, 1486, 1560
DpnII GATC 6 cut(s) 52, 223, 1153, 1158, 1484, 1558
EaeI YGGCCR 1 cut(s) 730
EciI GGCGGA 1 cut(s) 1393
Eco130I CCWWGG 6 cut(s) 869, 921, 1012, 1070, 1229, 1373
Eco47I GGWCC 3 cut(s) 77, 1008, 1370
Eco47III AGCGCT 1 cut(s) 201
Eco57I CTGAAG 3 cut(s) 636, 861, 1353
Eco81I CCTNAGG 1 cut(s) 963
EcoO109I RGGNCCY 2 cut(s) 77, 1370
EcoRII CCWGG 2 cut(s) 568, 819
EcoT14I CCWWGG 6 cut(s) 869, 921, 1012, 1070, 1229, 1373
ErhI CCWWGG 6 cut(s) 869, 921, 1012, 1070, 1229, 1373
Esp3I CGTCTC 1 cut(s) 184
FalI AAGNNNNNCTT 2 cut(s) 594, 626
FaqI GGGAC 9 cut(s) 31, 63, 707, 994, 1210, 1265, 1356, 1383, 1480
FbaI TGATCA 1 cut(s) 1558
FblI GTMKAC 1 cut(s) 1241
Fnu4HI GCNGC 7 cut(s) 10, 64, 110, 126, 296, 1084, 1275
FokI GGATG 2 cut(s) 785, 1212
Fsp4HI GCNGC 7 cut(s) 10, 64, 110, 126, 296, 1084, 1275
FspBI CTAG 1 cut(s) 1013
GlaI GCGC 3 cut(s) 34, 201, 355
GluI GCNGC 7 cut(s) 10, 64, 110, 126, 296, 1084, 1275
HaeII RGCGCY 2 cut(s) 36, 203
HaeIII GGCC 2 cut(s) 732, 1223
HapII CCGG 3 cut(s) 101, 274, 1550
HhaI GCGC 3 cut(s) 35, 202, 356
Hin1I GRCGYC 1 cut(s) 193
Hin6I GCGC 3 cut(s) 33, 200, 354
HinP1I GCGC 3 cut(s) 33, 200, 354
HincII GTYRAC 1 cut(s) 1389
HindII GTYRAC 1 cut(s) 1389
HindIII AAGCTT 1 cut(s) 740
HinfI GANTC 5 cut(s) 71, 145, 533, 824, 1184
HpaI GTTAAC 1 cut(s) 1389
HpaII CCGG 3 cut(s) 101, 274, 1550
HphI GGTGA 2 cut(s) 374, 1568
Hpy166II GTNNAC 3 cut(s) 764, 1242, 1389
Hpy188III TCNNGA 6 cut(s) 80, 530, 770, 947, 976, 1331
Hpy8I GTNNAC 3 cut(s) 764, 1242, 1389
Hpy99I CGWCG 3 cut(s) 86, 100, 198
HpyAV CCTTC 6 cut(s) 109, 173, 1063, 1144, 1259, 1509
HpyCH4III ACNGT 5 cut(s) 340, 891, 937, 1291, 1366
HpyCH4IV ACGT 3 cut(s) 84, 193, 304
HpyCH4V TGCA 6 cut(s) 175, 298, 485, 1277, 1474, 1534
HpyF10VI GCNNNNNNNGC 2 cut(s) 106, 125
HpyF3I CTNAG 4 cut(s) 437, 468, 963, 1245
HpySE526I ACGT 3 cut(s) 84, 193, 304
Hsp92I GRCGYC 1 cut(s) 193
HspAI GCGC 3 cut(s) 33, 200, 354
KflI GGGWCCC 1 cut(s) 77
Ksp22I TGATCA 1 cut(s) 1558
KspAI GTTAAC 1 cut(s) 1389
Kzo9I GATC 6 cut(s) 52, 223, 1153, 1158, 1484, 1558
LmnI GCTCC 2 cut(s) 1054, 1205
Lsp1109I GCAGC 2 cut(s) 282, 1261
LweI GCATC 3 cut(s) 423, 867, 1023
MabI ACCWGGT 1 cut(s) 568
MaeI CTAG 1 cut(s) 1013
MaeII ACGT 3 cut(s) 84, 193, 304
MaeIII GTNAC 4 cut(s) 103, 792, 891, 1163
MalI GATC 6 cut(s) 54, 225, 1155, 1160, 1486, 1560
MboI GATC 6 cut(s) 52, 223, 1153, 1158, 1484, 1558
MfeI CAATTG 1 cut(s) 1096
MflI RGATCY 2 cut(s) 1153, 1484
MlsI TGGCCA 1 cut(s) 732
MluCI AATT 8 cut(s) 332, 416, 1096, 1115, 1215, 1315, 1405, 1524
MluNI TGGCCA 1 cut(s) 732
MlyI GAGTC 2 cut(s) 80, 1193
MmeI TCCRAC 1 cut(s) 1276
Mox20I TGGCCA 1 cut(s) 732
MroXI GAANNNNTTC 2 cut(s) 864, 1326
MscI TGGCCA 1 cut(s) 732
MseI TTAA 5 cut(s) 335, 1218, 1388, 1425, 1443
Msp20I TGGCCA 1 cut(s) 732
MspI CCGG 3 cut(s) 101, 274, 1550
MspR9I CCNGG 3 cut(s) 275, 570, 821
MunI CAATTG 1 cut(s) 1096
MvaI CCWGG 2 cut(s) 570, 821
MvnI CGCG 2 cut(s) 109, 444
MwoI GCNNNNNNNGC 2 cut(s) 106, 125
NciI CCSGG 1 cut(s) 275
NcoI CCATGG 1 cut(s) 1229
NdeII GATC 6 cut(s) 52, 223, 1153, 1158, 1484, 1558
NlaIV GGNNCC 6 cut(s) 78, 79, 867, 960, 1010, 1371
NmeAIII GCCGAG 1 cut(s) 9
NmuCI GTSAC 2 cut(s) 103, 1163
NspI RCATGY 4 cut(s) 461, 1083, 1110, 1474
NspV TTCGAA 1 cut(s) 1320
PcsI WCGNNNNNNNCGW 3 cut(s) 66, 913, 1145
PdmI GAANNNNTTC 2 cut(s) 864, 1326
PfeI GAWTC 3 cut(s) 145, 533, 824
PkrI GCNGC 7 cut(s) 11, 65, 111, 127, 297, 1085, 1276
PleI GAGTC 2 cut(s) 79, 1192
PpsI GAGTC 2 cut(s) 79, 1192
PpuMI RGGWCCY 2 cut(s) 77, 1370
Psp5II RGGWCCY 2 cut(s) 77, 1370
Psp6I CCWGG 2 cut(s) 568, 819
PspGI CCWGG 2 cut(s) 568, 819
PspN4I GGNNCC 6 cut(s) 78, 79, 867, 960, 1010, 1371
PspPI GGNCC 4 cut(s) 77, 1008, 1222, 1370
PspPPI RGGWCCY 2 cut(s) 77, 1370
PstI CTGCAG 1 cut(s) 177
PsuI RGATCY 2 cut(s) 1153, 1484
RsaI GTAC 4 cut(s) 391, 510, 563, 1465
RsaNI GTAC 4 cut(s) 390, 509, 562, 1464
SaqAI TTAA 5 cut(s) 335, 1218, 1388, 1425, 1443
SatI GCNGC 7 cut(s) 10, 64, 110, 126, 296, 1084, 1275
Sau3AI GATC 6 cut(s) 52, 223, 1153, 1158, 1484, 1558
Sau96I GGNCC 4 cut(s) 77, 1008, 1222, 1370
ScaI AGTACT 1 cut(s) 563
SchI GAGTC 2 cut(s) 80, 1193
ScrFI CCNGG 3 cut(s) 275, 570, 821
SexAI ACCWGGT 1 cut(s) 568
SfaNI GCATC 3 cut(s) 423, 867, 1023
SfcI CTRYAG 1 cut(s) 173
SfuI TTCGAA 1 cut(s) 1320
SinI GGWCC 3 cut(s) 77, 1008, 1370
SmlI CTYRAG 1 cut(s) 974
SmoI CTYRAG 1 cut(s) 974
Sse9I AATT 8 cut(s) 332, 416, 1096, 1115, 1215, 1315, 1405, 1524
SsiI CCGC 8 cut(s) 9, 63, 109, 119, 125, 444, 1084, 1378
SspMI CTAG 1 cut(s) 1013
StyD4I CCNGG 3 cut(s) 273, 568, 819
StyI CCWWGG 6 cut(s) 869, 921, 1012, 1070, 1229, 1373
TaaI ACNGT 5 cut(s) 340, 891, 937, 1291, 1366
TaiI ACGT 3 cut(s) 87, 196, 307
TaqI TCGA 3 cut(s) 69, 196, 1320
TasI AATT 8 cut(s) 332, 416, 1096, 1115, 1215, 1315, 1405, 1524
TatI WGTACW 2 cut(s) 508, 561
TauI GCSGC 5 cut(s) 12, 66, 112, 128, 1086
TfiI GAWTC 3 cut(s) 145, 533, 824
Tru1I TTAA 5 cut(s) 335, 1218, 1388, 1425, 1443
Tru9I TTAA 5 cut(s) 335, 1218, 1388, 1425, 1443
TscAI CASTG 2 cut(s) 1253, 1371
TseFI GTSAC 2 cut(s) 103, 1163
TseI GCWGC 2 cut(s) 295, 1274
Tsp45I GTSAC 2 cut(s) 103, 1163
TspDTI ATGAA 8 cut(s) 127, 425, 493, 506, 677, 1140, 1278, 1399
TspRI CASTG 2 cut(s) 1253, 1371
VpaK11BI GGWCC 3 cut(s) 77, 1008, 1370
XapI RAATTY 2 cut(s) 1115, 1315
XceI RCATGY 4 cut(s) 461, 1083, 1110, 1474
XcmI CCANNNNNNNNNTGG 1 cut(s) 793
XmaJI CCTAGG 1 cut(s) 1012
XmiI GTMKAC 1 cut(s) 1241
XmnI GAANNNNTTC 2 cut(s) 864, 1326
XspI CTAG 1 cut(s) 1013
ZraI GACGTC 1 cut(s) 194
ZrmI AGTACT 1 cut(s) 563
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.