Rmu_sc0006237.1_g000004

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0006237.1
OG0006308
Physical Location & Seq
Reverse (-)
14242 .. 18307
4066 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0006237.1_g000004.1.cds

Sequence Viewer

Length: 1488 bp
atggaaggcggagacgggactatgcggctcggggctctcaacatgaaggcggatcgggtgaggttcgattcgagtcccgatgtgtcggtttcgtcgccagtgaccaagcagaaggcggcggcagccaagcagtttattgagaatcactacaagaactatctacaaggactacaggatcggcaagagagacgtcgagcacttcaaaggaaagtgcaagaagatcaggtaccggctgaggaacaagaagagatgatgaggaatttggcacgccgagaaacagaatatatgagactccaaagacgtaaaatcggaattgatgattttgagcaattaactgtaattggaaaaggtgcatttagcgaggttgaacatgtgcgttctgagagaaatttgctagctgaggttgatagtcggtgtattgtgaaactttattattcatttcaagattccgattacttataccttatcatggagtatttacctggtggtgacactatgacattattgatgagagaagatactctttctgaagatgttgcacgtttttatatgggagagagtattctggctattcactcaatccatcaacacaactatgttcacagggacataaaaccagacaatctgatactggataaaactggccatttgaagctttcagattttggcttgtgtaaacctctggatgacaagtattctacattattggaaaatgaggatttaacatctcaagaatgcatagctgaaactgaaggacaatctggctgtgataaggttccttggttgatgccaaaagaacaattacaacaatggaaacgtaatcggcgtgccttggcttattcaactgttggaactcttgactacatggcacccgaggtttttctgaagaaaggctatggtatagagtgtgattggtggtcactaggagcaatcatgtacgagatgctagtaggctatcctccattctgctctgatgatccaaggatcacatgccgcaagattatcaattggagaacatgcctgaagtttcccgatgagccaaaaatttcagacgaggcaagggatttgatctgtcacttgttatgtgatgttgaagcaaggcttgggacacatggagtagaagaaattaaggcacatccttggttcagaggtattgagtgggacaggctatatgaaattgaagctgcgtataaacctacagttgttggagacttggacactcagaatttcgagaagtttcctgatctagaaggtgcaccatctgcaacaccaacagtgggaccttggcggaagatgttaacctcaaaagataacaatttcattggatacactttcaagaaatcagatgtccttaaatcactcgaaagttcaggtacagacatgagatcaaatggatcttcaaagtccccatcactgatttccttgttaggtagaattgacttgcaagacagtgtaataccagagggcgaaccaaagcaagaaggctaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000166 GO:0000902 GO:0000904 GO:0002009 GO:0002165 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005488 GO:0005524 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005938 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0007275 GO:0007399 GO:0007423 GO:0007444 GO:0007455 GO:0007469 GO:0007472 GO:0007476 GO:0007552 GO:0007560 GO:0008037 GO:0008038 GO:0008144 GO:0008150 GO:0008152 GO:0008544 GO:0009653 GO:0009791 GO:0009886 GO:0009887 GO:0009888 GO:0009913 GO:0009987 GO:0010769 GO:0010975 GO:0016043 GO:0016301 GO:0016310 GO:0016358 GO:0016740 GO:0016772 GO:0016773 GO:0017076 GO:0018105 GO:0018193 GO:0018209 GO:0019538 GO:0022008 GO:0022416 GO:0022603 GO:0022604 GO:0023052 GO:0030030 GO:0030154 GO:0030182 GO:0030424 GO:0030425 GO:0030554 GO:0030855 GO:0031175 GO:0031344 GO:0032501 GO:0032502 GO:0032553 GO:0032555 GO:0032559 GO:0032989 GO:0032990 GO:0035107 GO:0035114 GO:0035120 GO:0035214 GO:0035220 GO:0035239 GO:0035295 GO:0035315 GO:0035316 GO:0035317 GO:0035556 GO:0035639 GO:0036094 GO:0036211 GO:0036477 GO:0042995 GO:0043005 GO:0043167 GO:0043168 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044297 GO:0044424 GO:0044444 GO:0044463 GO:0044464 GO:0045595 GO:0045664 GO:0048468 GO:0048513 GO:0048563 GO:0048569 GO:0048666 GO:0048667 GO:0048699 GO:0048707 GO:0048729 GO:0048731 GO:0048736 GO:0048737 GO:0048800 GO:0048812 GO:0048813 GO:0048814 GO:0048856 GO:0048858 GO:0048869 GO:0050767 GO:0050773 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051128 GO:0051239 GO:0051716 GO:0051960 GO:0060284 GO:0060429 GO:0060562 GO:0065007 GO:0070451 GO:0070593 GO:0071704 GO:0071840 GO:0071944 GO:0097159 GO:0097367 GO:0097447 GO:0097458 GO:0099568 GO:0120025 GO:0120035 GO:0120036 GO:0120038 GO:0120039 GO:0140096 GO:1901265 GO:1901363 GO:1901564 GO:2000026
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase Pkinase_C

Protein Analysis

495

Amino Acids

56.76

Weight (kDa)

5.28

Isoelectric Point (pI)

52.63

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 193
Acc65I GGTACC 1 cut(s) 226
AccB1I GGYRCC 2 cut(s) 226, 868
AciI CCGC 7 cut(s) 9, 25, 50, 116, 119, 994, 1288
AclWI GGATC 5 cut(s) 60, 183, 971, 992, 1402
AcoI YGGCCR 1 cut(s) 643
AcsI RAATTY 4 cut(s) 259, 388, 1044, 1225
AcuI CTGAAG 4 cut(s) 549, 771, 905, 1043
AcyI GRCGYC 1 cut(s) 190
AfaI GTAC 3 cut(s) 228, 938, 1375
AfiI CCNNNNNNNGG 2 cut(s) 469, 1277
AflIII ACRYGT 1 cut(s) 370
AgsI TTSAA 9 cut(s) 203, 368, 443, 652, 843, 1094, 1181, 1336, 1401
AjnI CCWGG 1 cut(s) 481
AjuI GAANNNNNNNTTGG 2 cut(s) 1086, 1118
AluBI AGCT 4 cut(s) 398, 655, 743, 1184
AluI AGCT 4 cut(s) 398, 655, 743, 1184
Alw21I GWGCWC 2 cut(s) 199, 1258
Alw26I GTCTC 4 cut(s) 6, 181, 283, 1203
Alw44I GTGCAC 1 cut(s) 1254
AlwI GGATC 5 cut(s) 60, 183, 971, 992, 1402
Ama87I CYCGRG 2 cut(s) 29, 872
AoxI GGCC 1 cut(s) 643
ApaLI GTGCAC 1 cut(s) 1254
ApeKI GCWGC 2 cut(s) 122, 1184
ApoI RAATTY 4 cut(s) 259, 388, 1044, 1225
Asp718I GGTACC 1 cut(s) 226
AspS9I GGNCC 1 cut(s) 1280
AsuHPI GGTGA 2 cut(s) 70, 500
AsuNHI GCTAGC 1 cut(s) 394
AvaI CYCGRG 2 cut(s) 29, 872
AvaII GGWCC 1 cut(s) 1280
BaeGI GKGCMC 1 cut(s) 1258
BalI TGGCCA 1 cut(s) 645
BanI GGYRCC 2 cut(s) 226, 868
BanII GRGCYC 1 cut(s) 37
BarI GAAGNNNNNNTAC 2 cut(s) 210, 242
Bbv12I GWGCWC 2 cut(s) 199, 1258
BbvCI CCTCAGC 2 cut(s) 234, 399
BbvI GCAGC 2 cut(s) 134, 1171
BccI CCATC 3 cut(s) 591, 1267, 1417
BciT130I CCWGG 1 cut(s) 483
BciVI GTATCC 1 cut(s) 1319
BcoDI GTCTC 4 cut(s) 6, 181, 283, 1203
BfaI CTAG 4 cut(s) 395, 923, 947, 1247
BfmI CTRYAG 2 cut(s) 170, 1197
BfuI GTATCC 1 cut(s) 1319
BisI GCNGC 6 cut(s) 26, 117, 120, 123, 994, 1185
BlsI GCNGC 6 cut(s) 27, 118, 121, 124, 995, 1186
Bme1390I CCNGG 1 cut(s) 483
Bme18I GGWCC 1 cut(s) 1280
BmeT110I CYCGRG 2 cut(s) 29, 872
BmgT120I GGNCC 1 cut(s) 1280
BmiI GGNNCC 4 cut(s) 228, 777, 870, 1281
BmrFI CCNGG 1 cut(s) 483
BmsI GCATC 2 cut(s) 777, 933
BmtI GCTAGC 1 cut(s) 398
Bpu10I CCTNAGC 2 cut(s) 234, 399
BpuEI CTTGAG 1 cut(s) 714
BsaHI GRCGYC 1 cut(s) 190
BsaJI CCNNGG 6 cut(s) 779, 831, 873, 980, 1139, 1283
Bsc4I CCNNNNNNNGG 2 cut(s) 469, 1277
Bse118I RCCGGY 1 cut(s) 229
Bse1I ACTGG 3 cut(s) 98, 636, 646
BseBI CCWGG 1 cut(s) 483
BseDI CCNNGG 6 cut(s) 779, 831, 873, 980, 1139, 1283
BseGI GGATG 2 cut(s) 691, 1135
BseLI CCNNNNNNNGG 2 cut(s) 469, 1277
BseMII CTCAG 4 cut(s) 225, 372, 390, 1235
BseNI ACTGG 3 cut(s) 98, 636, 646
BseSI GKGCMC 1 cut(s) 1258
BseXI GCAGC 2 cut(s) 134, 1171
BshFI GGCC 1 cut(s) 645
BshNI GGYRCC 2 cut(s) 226, 868
BsiHKAI GWGCWC 2 cut(s) 199, 1258
BsiHKCI CYCGRG 2 cut(s) 29, 872
BsiSI CCGG 1 cut(s) 230
BslFI GGGAC 7 cut(s) 31, 60, 620, 1120, 1175, 1293, 1390
BslI CCNNNNNNNGG 2 cut(s) 469, 1277
BsmAI GTCTC 4 cut(s) 6, 181, 283, 1203
BsmBI CGTCTC 2 cut(s) 6, 181
BsmFI GGGAC 7 cut(s) 31, 60, 620, 1120, 1175, 1293, 1390
BsmI GAATGC 1 cut(s) 740
BsnI GGCC 1 cut(s) 645
BsoBI CYCGRG 2 cut(s) 29, 872
Bsp1286I GDGCHC 3 cut(s) 37, 199, 1258
Bsp143I GATC 9 cut(s) 52, 175, 220, 976, 984, 1068, 1243, 1385, 1394
BspACI CCGC 7 cut(s) 9, 25, 50, 116, 119, 994, 1288
BspANI GGCC 1 cut(s) 645
BspCNI CTCAG 4 cut(s) 226, 373, 391, 1234
BspLI GGNNCC 4 cut(s) 228, 777, 870, 1281
BspOI GCTAGC 1 cut(s) 398
BspPI GGATC 5 cut(s) 60, 183, 971, 992, 1402
BspT107I GGYRCC 2 cut(s) 226, 868
BsrFI RCCGGY 1 cut(s) 229
BsrI ACTGG 3 cut(s) 98, 636, 646
BssAI RCCGGY 1 cut(s) 229
BssECI CCNNGG 6 cut(s) 779, 831, 873, 980, 1139, 1283
BssMI GATC 9 cut(s) 52, 175, 220, 976, 984, 1068, 1243, 1385, 1394
BssNI GRCGYC 1 cut(s) 190
BssT1I CCWWGG 5 cut(s) 779, 831, 980, 1139, 1283
Bst2UI CCWGG 1 cut(s) 483
Bst4CI ACNGT 5 cut(s) 337, 847, 1201, 1276, 1451
Bst6I CTCTTC 1 cut(s) 240
BstACI GRCGYC 1 cut(s) 190
BstAPI GCANNNNNTGC 1 cut(s) 1262
BstC8I GCNNGC 3 cut(s) 268, 396, 828
BstDEI CTNAG 4 cut(s) 234, 381, 399, 1221
BstF5I GGATG 2 cut(s) 691, 1135
BstKTI GATC 9 cut(s) 55, 178, 223, 979, 987, 1071, 1246, 1388, 1397
BstMAI GTCTC 4 cut(s) 6, 181, 283, 1203
BstMBI GATC 9 cut(s) 52, 175, 220, 976, 984, 1068, 1243, 1385, 1394
BstMWI GCNNNNNNNGC 2 cut(s) 122, 1262
BstNI CCWGG 1 cut(s) 483
BstNSI RCATGY 3 cut(s) 374, 993, 1020
BstSCI CCNGG 1 cut(s) 481
BstSFI CTRYAG 2 cut(s) 170, 1197
BstSLI GKGCMC 1 cut(s) 1258
BstV1I GCAGC 2 cut(s) 134, 1171
BstX2I RGATCY 1 cut(s) 1394
BstYI RGATCY 1 cut(s) 1394
BsuI GTATCC 1 cut(s) 1319
BsuRI GGCC 1 cut(s) 645
BtsCI GGATG 2 cut(s) 691, 1135
BtsIMutI CAGTG 4 cut(s) 105, 1281, 1412, 1456
Cac8I GCNNGC 3 cut(s) 268, 396, 828
Cfr10I RCCGGY 1 cut(s) 229
Cfr13I GGNCC 1 cut(s) 1280
CsiI ACCWGGT 1 cut(s) 481
Csp6I GTAC 3 cut(s) 227, 937, 1374
CviAII CATG 9 cut(s) 43, 371, 469, 865, 934, 990, 1017, 1112, 1381
CviQI GTAC 3 cut(s) 227, 937, 1374
DdeI CTNAG 4 cut(s) 234, 381, 399, 1221
DpnI GATC 9 cut(s) 54, 177, 222, 978, 986, 1070, 1245, 1387, 1396
DpnII GATC 9 cut(s) 52, 175, 220, 976, 984, 1068, 1243, 1385, 1394
EaeI YGGCCR 1 cut(s) 643
Eam1104I CTCTTC 1 cut(s) 240
EarI CTCTTC 1 cut(s) 240
EciI GGCGGA 3 cut(s) 24, 65, 1303
Eco130I CCWWGG 5 cut(s) 779, 831, 980, 1139, 1283
Eco24I GRGCYC 1 cut(s) 37
Eco47I GGWCC 1 cut(s) 1280
Eco57I CTGAAG 4 cut(s) 549, 771, 905, 1043
Eco88I CYCGRG 2 cut(s) 29, 872
EcoO109I RGGNCCY 1 cut(s) 1280
EcoRII CCWGG 1 cut(s) 481
EcoT14I CCWWGG 5 cut(s) 779, 831, 980, 1139, 1283
EcoT22I ATGCAT 1 cut(s) 740
EcoT38I GRGCYC 1 cut(s) 37
ErhI CCWWGG 5 cut(s) 779, 831, 980, 1139, 1283
Esp3I CGTCTC 2 cut(s) 6, 181
FaeI CATG 9 cut(s) 46, 374, 472, 868, 937, 993, 1020, 1115, 1384
FalI AAGNNNNNCTT 4 cut(s) 507, 539, 1086, 1118
FaqI GGGAC 7 cut(s) 31, 60, 620, 1120, 1175, 1293, 1390
FatI CATG 9 cut(s) 42, 370, 468, 864, 933, 989, 1016, 1111, 1380
Fnu4HI GCNGC 6 cut(s) 26, 117, 120, 123, 994, 1185
FokI GGATG 2 cut(s) 698, 1122
FriOI GRGCYC 1 cut(s) 37
Fsp4HI GCNGC 6 cut(s) 26, 117, 120, 123, 994, 1185
FspBI CTAG 4 cut(s) 395, 923, 947, 1247
GluI GCNGC 6 cut(s) 26, 117, 120, 123, 994, 1185
HaeIII GGCC 1 cut(s) 645
HapII CCGG 1 cut(s) 230
Hin1I GRCGYC 1 cut(s) 190
Hin1II CATG 9 cut(s) 46, 374, 472, 868, 937, 993, 1020, 1115, 1384
HincII GTYRAC 1 cut(s) 1299
HindII GTYRAC 1 cut(s) 1299
HindIII AAGCTT 1 cut(s) 653
HinfI GANTC 5 cut(s) 68, 73, 142, 291, 446
HpaI GTTAAC 1 cut(s) 1299
HpaII CCGG 1 cut(s) 230
HphI GGTGA 2 cut(s) 70, 500
Hpy166II GTNNAC 4 cut(s) 601, 677, 1256, 1299
Hpy8I GTNNAC 4 cut(s) 601, 677, 1256, 1299
Hpy99I CGWCG 2 cut(s) 97, 195
HpyAV CCTTC 5 cut(s) 40, 106, 746, 1244, 1475
HpyCH4III ACNGT 5 cut(s) 337, 847, 1201, 1276, 1451
HpyCH4IV ACGT 4 cut(s) 190, 301, 541, 817
HpyCH4V TGCA 7 cut(s) 214, 353, 539, 738, 1256, 1265, 1444
HpyF10VI GCNNNNNNNGC 2 cut(s) 122, 1262
HpyF3I CTNAG 4 cut(s) 234, 381, 399, 1221
HpySE526I ACGT 4 cut(s) 190, 301, 541, 817
Hsp92I GRCGYC 1 cut(s) 190
Hsp92II CATG 9 cut(s) 46, 374, 472, 868, 937, 993, 1020, 1115, 1384
KpnI GGTACC 1 cut(s) 230
KspAI GTTAAC 1 cut(s) 1299
Kzo9I GATC 9 cut(s) 52, 175, 220, 976, 984, 1068, 1243, 1385, 1394
LmnI GCTCC 1 cut(s) 926
Lsp1109I GCAGC 2 cut(s) 134, 1171
LweI GCATC 2 cut(s) 777, 933
MabI ACCWGGT 1 cut(s) 481
MaeI CTAG 4 cut(s) 395, 923, 947, 1247
MaeII ACGT 4 cut(s) 190, 301, 541, 817
MaeIII GTNAC 4 cut(s) 100, 488, 918, 1073
MalI GATC 9 cut(s) 54, 177, 222, 978, 986, 1070, 1245, 1387, 1396
MboI GATC 9 cut(s) 52, 175, 220, 976, 984, 1068, 1243, 1385, 1394
MboII GAAGA 8 cut(s) 230, 257, 527, 542, 898, 1133, 1303, 1389
MfeI CAATTG 1 cut(s) 1006
MflI RGATCY 1 cut(s) 1394
MhlI GDGCHC 3 cut(s) 37, 199, 1258
MlsI TGGCCA 1 cut(s) 645
MluNI TGGCCA 1 cut(s) 645
MlyI GAGTC 2 cut(s) 82, 285
MmeI TCCRAC 2 cut(s) 829, 1186
Mox20I TGGCCA 1 cut(s) 645
Mph1103I ATGCAT 1 cut(s) 740
MscI TGGCCA 1 cut(s) 645
MseI TTAA 5 cut(s) 332, 722, 1128, 1298, 1353
MslI CAYNNNNRTG 1 cut(s) 594
Msp20I TGGCCA 1 cut(s) 645
MspI CCGG 1 cut(s) 230
MspR9I CCNGG 1 cut(s) 483
MunI CAATTG 1 cut(s) 1006
Mva1269I GAATGC 1 cut(s) 740
MvaI CCWGG 1 cut(s) 483
MwoI GCNNNNNNNGC 2 cut(s) 122, 1262
NdeII GATC 9 cut(s) 52, 175, 220, 976, 984, 1068, 1243, 1385, 1394
NheI GCTAGC 1 cut(s) 394
NlaIII CATG 9 cut(s) 46, 374, 472, 868, 937, 993, 1020, 1115, 1384
NlaIV GGNNCC 4 cut(s) 228, 777, 870, 1281
NmeAIII GCCGAG 1 cut(s) 296
NmuCI GTSAC 4 cut(s) 100, 488, 918, 1073
NsiI ATGCAT 1 cut(s) 740
NspI RCATGY 3 cut(s) 374, 993, 1020
PciI ACATGT 1 cut(s) 370
PcsI WCGNNNNNNNCGW 1 cut(s) 823
PctI GAATGC 1 cut(s) 740
PfeI GAWTC 3 cut(s) 68, 142, 446
PkrI GCNGC 6 cut(s) 27, 118, 121, 124, 995, 1186
PleI GAGTC 2 cut(s) 81, 285
PpsI GAGTC 2 cut(s) 81, 285
PpuMI RGGWCCY 1 cut(s) 1280
PscI ACATGT 1 cut(s) 370
Psp5II RGGWCCY 1 cut(s) 1280
Psp6I CCWGG 1 cut(s) 481
PspGI CCWGG 1 cut(s) 481
PspN4I GGNNCC 4 cut(s) 228, 777, 870, 1281
PspPI GGNCC 1 cut(s) 1280
PspPPI RGGWCCY 1 cut(s) 1280
PsuI RGATCY 1 cut(s) 1394
RsaI GTAC 3 cut(s) 228, 938, 1375
RsaNI GTAC 3 cut(s) 227, 937, 1374
RseI CAYNNNNRTG 1 cut(s) 594
SaqAI TTAA 5 cut(s) 332, 722, 1128, 1298, 1353
SatI GCNGC 6 cut(s) 26, 117, 120, 123, 994, 1185
Sau3AI GATC 9 cut(s) 52, 175, 220, 976, 984, 1068, 1243, 1385, 1394
Sau96I GGNCC 1 cut(s) 1280
SchI GAGTC 2 cut(s) 82, 285
ScrFI CCNGG 1 cut(s) 483
SduI GDGCHC 3 cut(s) 37, 199, 1258
SexAI ACCWGGT 1 cut(s) 481
SfaNI GCATC 2 cut(s) 777, 933
SfcI CTRYAG 2 cut(s) 170, 1197
SinI GGWCC 1 cut(s) 1280
SmiMI CAYNNNNRTG 1 cut(s) 594
SmlI CTYRAG 1 cut(s) 729
SmoI CTYRAG 1 cut(s) 729
SsiI CCGC 7 cut(s) 9, 25, 50, 116, 119, 994, 1288
SspMI CTAG 4 cut(s) 395, 923, 947, 1247
StyD4I CCNGG 1 cut(s) 481
StyI CCWWGG 5 cut(s) 779, 831, 980, 1139, 1283
TaaI ACNGT 5 cut(s) 337, 847, 1201, 1276, 1451
TaiI ACGT 4 cut(s) 193, 304, 544, 820
TaqI TCGA 5 cut(s) 66, 71, 193, 1230, 1362
TauI GCSGC 4 cut(s) 28, 119, 122, 996
TfiI GAWTC 3 cut(s) 68, 142, 446
Tru1I TTAA 5 cut(s) 332, 722, 1128, 1298, 1353
Tru9I TTAA 5 cut(s) 332, 722, 1128, 1298, 1353
TscAI CASTG 4 cut(s) 105, 1281, 1419, 1456
TseFI GTSAC 4 cut(s) 100, 488, 918, 1073
TseI GCWGC 2 cut(s) 122, 1184
Tsp45I GTSAC 4 cut(s) 100, 488, 918, 1073
TspDTI ATGAA 4 cut(s) 59, 426, 1188, 1309
TspRI CASTG 4 cut(s) 105, 1281, 1419, 1456
VneI GTGCAC 1 cut(s) 1254
VpaK11BI GGWCC 1 cut(s) 1280
XapI RAATTY 4 cut(s) 259, 388, 1044, 1225
XbaI TCTAGA 1 cut(s) 1246
XceI RCATGY 3 cut(s) 374, 993, 1020
XspI CTAG 4 cut(s) 395, 923, 947, 1247
ZraI GACGTC 1 cut(s) 191
Zsp2I ATGCAT 1 cut(s) 740
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.