Rmu_sc0006237.1_g000005

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0006237.1
OG0006308
Physical Location & Seq
Reverse (-)
19981 .. 23396
3416 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0006237.1_g000005.1.cds

Sequence Viewer

Length: 1473 bp
atggaaggcggagacgggacgatgcggcttggggctctcaacatgaaggcggatcgggtgaggttcgattcgagtcccgatgtgtcggtttcgtcgccggtgaccaagcagaaggcggcggcggccaagcagtttattgagaatcactacaagaactatctacaaggactacaggatcggcaagagagacgtcgagcacttcaaaggaaagtgcaagaagatcaggtaccggctgaggaacaagaagagatgatgaggaatttggcacgccgagaaacagaatatatgagactccaaagacgtaaaatcggaattgatgattttgagcaattaactgtaattggaaaaggtgcatttagcgaggtaaggttatgccgtgcaaaaggtacaggagagattttcgctatgaagaaattgaagaaatcagagatgcttagccgtggacaggttgaacatgtgcgttctgagagaaatttgcttgctgaggttgatagtcggtgtattgtgaaactttattattcatttcaagattccgatttcttataccttatcatggagtatttacccggtggtgacattatgacattattgatgagagaagatactctttctgaagatgttgcacgtttttatatgggagagagtattctggctattcactcaatccatcaacacaactatgttcacagggacataaaaccagacaatctgatactggataaaactggccatttgaagctttcagattttggcttgtgtaaacctctggatgacaagtattctacattattggaaaatgaggatttaacatctcaagaatgcatagctgaaactgaaggacaatctggctgtgataaggttccttggttgatgccaaaagaacaattacaacaatggaaacgtaatcggcgtgccttggcttattcaactgttggaactcttgactacatggcacccgaggttttgctgaagaaaggatatggtatggagtgtgattggtggtcactaggagcaatcatgtacgagatgctagtaggctatcctccattctgctctgatgatccaaggatcacatgccgcaagattatcaattggagaacatgcctgaagtttcccgatgagccaaaaatttcagacgaggcaagggatttgatctgtcacttgttatgtgatgttgaaacaaggcttgggacacatggagtagaagaaattaaggcacatccttggttcagaggtattgagtgggacaggctatatgaaattgaagctgcgtataaacctacagttgttggagacttggacactcagaatttcgagaagtttcctgatctagaaggtgcaccatctgcaacaccaacagtgggcccttggcggaagatgttaacctcgaaagataacaatttcattggatacactttcaagaaatcagatgtccttaaatcacttgaaagttcaggtcggctgcttctctag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000166 GO:0000902 GO:0000904 GO:0002009 GO:0002165 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005488 GO:0005524 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005938 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0007275 GO:0007399 GO:0007423 GO:0007444 GO:0007455 GO:0007469 GO:0007472 GO:0007476 GO:0007552 GO:0007560 GO:0008037 GO:0008038 GO:0008144 GO:0008150 GO:0008152 GO:0008544 GO:0009653 GO:0009791 GO:0009886 GO:0009887 GO:0009888 GO:0009913 GO:0009987 GO:0010769 GO:0010975 GO:0016043 GO:0016301 GO:0016310 GO:0016358 GO:0016740 GO:0016772 GO:0016773 GO:0017076 GO:0018105 GO:0018193 GO:0018209 GO:0019538 GO:0022008 GO:0022416 GO:0022603 GO:0022604 GO:0023052 GO:0030030 GO:0030154 GO:0030182 GO:0030424 GO:0030425 GO:0030554 GO:0030855 GO:0031175 GO:0031344 GO:0032501 GO:0032502 GO:0032553 GO:0032555 GO:0032559 GO:0032989 GO:0032990 GO:0035107 GO:0035114 GO:0035120 GO:0035214 GO:0035220 GO:0035239 GO:0035295 GO:0035315 GO:0035316 GO:0035317 GO:0035556 GO:0035639 GO:0036094 GO:0036211 GO:0036477 GO:0042995 GO:0043005 GO:0043167 GO:0043168 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044297 GO:0044424 GO:0044444 GO:0044463 GO:0044464 GO:0045595 GO:0045664 GO:0048468 GO:0048513 GO:0048563 GO:0048569 GO:0048666 GO:0048667 GO:0048699 GO:0048707 GO:0048729 GO:0048731 GO:0048736 GO:0048737 GO:0048800 GO:0048812 GO:0048813 GO:0048814 GO:0048856 GO:0048858 GO:0048869 GO:0050767 GO:0050773 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051128 GO:0051239 GO:0051716 GO:0051960 GO:0060284 GO:0060429 GO:0060562 GO:0065007 GO:0070451 GO:0070593 GO:0071704 GO:0071840 GO:0071944 GO:0097159 GO:0097367 GO:0097447 GO:0097458 GO:0099568 GO:0120025 GO:0120035 GO:0120036 GO:0120038 GO:0120039 GO:0140096 GO:1901265 GO:1901363 GO:1901564 GO:2000026
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase Pkinase_C

Protein Analysis

490

Amino Acids

56.49

Weight (kDa)

5.93

Isoelectric Point (pI)

47.52

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 193
Acc65I GGTACC 1 cut(s) 226
AccB1I GGYRCC 2 cut(s) 226, 952
AciI CCGC 8 cut(s) 9, 25, 50, 116, 119, 122, 1078, 1372
AclWI GGATC 4 cut(s) 60, 183, 1055, 1076
AcoI YGGCCR 2 cut(s) 123, 727
AcsI RAATTY 4 cut(s) 259, 472, 1128, 1309
AcuI CTGAAG 4 cut(s) 633, 855, 989, 1127
AcyI GRCGYC 1 cut(s) 190
AfaI GTAC 3 cut(s) 228, 388, 1022
AfiI CCNNNNNNNGG 3 cut(s) 445, 553, 1361
AflIII ACRYGT 1 cut(s) 454
AjuI GAANNNNNNNTTGG 2 cut(s) 1170, 1202
AluBI AGCT 3 cut(s) 739, 827, 1268
AluI AGCT 3 cut(s) 739, 827, 1268
Alw21I GWGCWC 2 cut(s) 199, 1342
Alw26I GTCTC 4 cut(s) 6, 181, 283, 1287
Alw44I GTGCAC 1 cut(s) 1338
AlwI GGATC 4 cut(s) 60, 183, 1055, 1076
Ama87I CYCGRG 1 cut(s) 956
AoxI GGCC 3 cut(s) 123, 727, 1363
ApaI GGGCCC 1 cut(s) 1367
ApaLI GTGCAC 1 cut(s) 1338
ApeKI GCWGC 2 cut(s) 1268, 1462
ApoI RAATTY 4 cut(s) 259, 472, 1128, 1309
Asp718I GGTACC 1 cut(s) 226
AspS9I GGNCC 2 cut(s) 1363, 1364
AsuC2I CCSGG 1 cut(s) 567
AsuHPI GGTGA 3 cut(s) 70, 112, 584
AvaI CYCGRG 1 cut(s) 956
BaeGI GKGCMC 2 cut(s) 1342, 1367
BalI TGGCCA 1 cut(s) 729
BanI GGYRCC 2 cut(s) 226, 952
BanII GRGCYC 2 cut(s) 37, 1367
BarI GAAGNNNNNNTAC 2 cut(s) 210, 242
Bbv12I GWGCWC 2 cut(s) 199, 1342
BbvCI CCTCAGC 2 cut(s) 234, 483
BbvI GCAGC 2 cut(s) 1255, 1449
BccI CCATC 2 cut(s) 675, 1351
BceAI ACGGC 2 cut(s) 360, 423
BciVI GTATCC 1 cut(s) 1403
BcnI CCSGG 1 cut(s) 567
BcoDI GTCTC 4 cut(s) 6, 181, 283, 1287
BfaI CTAG 4 cut(s) 1007, 1031, 1331, 1471
BfmI CTRYAG 2 cut(s) 170, 1281
BfuI GTATCC 1 cut(s) 1403
BisI GCNGC 7 cut(s) 26, 117, 120, 123, 1078, 1269, 1463
BlpI GCTNAGC 1 cut(s) 434
BlsI GCNGC 7 cut(s) 27, 118, 121, 124, 1079, 1270, 1464
Bme1390I CCNGG 1 cut(s) 567
BmeT110I CYCGRG 1 cut(s) 956
BmgT120I GGNCC 2 cut(s) 1363, 1364
BmiI GGNNCC 4 cut(s) 228, 861, 954, 1365
BmrFI CCNGG 1 cut(s) 567
BmsI GCATC 4 cut(s) 12, 420, 861, 1017
Bpu10I CCTNAGC 2 cut(s) 234, 483
Bpu1102I GCTNAGC 1 cut(s) 434
BpuEI CTTGAG 1 cut(s) 798
BpuMI CCSGG 1 cut(s) 567
BsaHI GRCGYC 1 cut(s) 190
BsaJI CCNNGG 7 cut(s) 439, 863, 915, 957, 1064, 1223, 1367
Bsc4I CCNNNNNNNGG 3 cut(s) 445, 553, 1361
Bse118I RCCGGY 2 cut(s) 97, 229
Bse1I ACTGG 2 cut(s) 720, 730
BseDI CCNNGG 7 cut(s) 439, 863, 915, 957, 1064, 1223, 1367
BseGI GGATG 2 cut(s) 775, 1219
BseLI CCNNNNNNNGG 3 cut(s) 445, 553, 1361
BseMII CTCAG 4 cut(s) 225, 456, 474, 1319
BseNI ACTGG 2 cut(s) 720, 730
BseSI GKGCMC 2 cut(s) 1342, 1367
BseXI GCAGC 2 cut(s) 1255, 1449
BshFI GGCC 3 cut(s) 125, 729, 1365
BshNI GGYRCC 2 cut(s) 226, 952
BsiHKAI GWGCWC 2 cut(s) 199, 1342
BsiHKCI CYCGRG 1 cut(s) 956
BsiSI CCGG 3 cut(s) 98, 230, 567
BslFI GGGAC 5 cut(s) 31, 60, 704, 1204, 1259
BslI CCNNNNNNNGG 3 cut(s) 445, 553, 1361
BsmAI GTCTC 4 cut(s) 6, 181, 283, 1287
BsmBI CGTCTC 2 cut(s) 6, 181
BsmFI GGGAC 5 cut(s) 31, 60, 704, 1204, 1259
BsmI GAATGC 1 cut(s) 824
BsnI GGCC 3 cut(s) 125, 729, 1365
BsoBI CYCGRG 1 cut(s) 956
Bsp120I GGGCCC 1 cut(s) 1363
Bsp1286I GDGCHC 4 cut(s) 37, 199, 1342, 1367
Bsp143I GATC 7 cut(s) 52, 175, 220, 1060, 1068, 1152, 1327
Bsp1720I GCTNAGC 1 cut(s) 434
BspACI CCGC 8 cut(s) 9, 25, 50, 116, 119, 122, 1078, 1372
BspANI GGCC 3 cut(s) 125, 729, 1365
BspCNI CTCAG 4 cut(s) 226, 457, 475, 1318
BspLI GGNNCC 4 cut(s) 228, 861, 954, 1365
BspPI GGATC 4 cut(s) 60, 183, 1055, 1076
BspT107I GGYRCC 2 cut(s) 226, 952
BsrFI RCCGGY 2 cut(s) 97, 229
BsrI ACTGG 2 cut(s) 720, 730
BssAI RCCGGY 2 cut(s) 97, 229
BssECI CCNNGG 7 cut(s) 439, 863, 915, 957, 1064, 1223, 1367
BssMI GATC 7 cut(s) 52, 175, 220, 1060, 1068, 1152, 1327
BssNI GRCGYC 1 cut(s) 190
BssT1I CCWWGG 5 cut(s) 863, 915, 1064, 1223, 1367
Bst4CI ACNGT 4 cut(s) 337, 931, 1285, 1360
Bst6I CTCTTC 1 cut(s) 240
BstACI GRCGYC 1 cut(s) 190
BstAPI GCANNNNNTGC 1 cut(s) 1346
BstC8I GCNNGC 3 cut(s) 268, 480, 912
BstDEI CTNAG 5 cut(s) 234, 434, 465, 483, 1305
BstDSI CCRYGG 1 cut(s) 439
BstEII GGTNACC 1 cut(s) 100
BstF5I GGATG 2 cut(s) 775, 1219
BstKTI GATC 7 cut(s) 55, 178, 223, 1063, 1071, 1155, 1330
BstMAI GTCTC 4 cut(s) 6, 181, 283, 1287
BstMBI GATC 7 cut(s) 52, 175, 220, 1060, 1068, 1152, 1327
BstMWI GCNNNNNNNGC 2 cut(s) 122, 1346
BstNSI RCATGY 3 cut(s) 458, 1077, 1104
BstPI GGTNACC 1 cut(s) 100
BstSCI CCNGG 1 cut(s) 565
BstSFI CTRYAG 2 cut(s) 170, 1281
BstSLI GKGCMC 2 cut(s) 1342, 1367
BstV1I GCAGC 2 cut(s) 1255, 1449
BsuI GTATCC 1 cut(s) 1403
BsuRI GGCC 3 cut(s) 125, 729, 1365
BtgI CCRYGG 1 cut(s) 439
BtsCI GGATG 2 cut(s) 775, 1219
BtsIMutI CAGTG 1 cut(s) 1365
Cac8I GCNNGC 3 cut(s) 268, 480, 912
Cfr10I RCCGGY 2 cut(s) 97, 229
Cfr13I GGNCC 2 cut(s) 1363, 1364
Csp6I GTAC 3 cut(s) 227, 387, 1021
CviAII CATG 8 cut(s) 43, 455, 553, 949, 1018, 1074, 1101, 1196
CviQI GTAC 3 cut(s) 227, 387, 1021
DdeI CTNAG 5 cut(s) 234, 434, 465, 483, 1305
DpnI GATC 7 cut(s) 54, 177, 222, 1062, 1070, 1154, 1329
DpnII GATC 7 cut(s) 52, 175, 220, 1060, 1068, 1152, 1327
EaeI YGGCCR 2 cut(s) 123, 727
Eam1104I CTCTTC 1 cut(s) 240
EarI CTCTTC 1 cut(s) 240
EciI GGCGGA 3 cut(s) 24, 65, 1387
Eco130I CCWWGG 5 cut(s) 863, 915, 1064, 1223, 1367
Eco24I GRGCYC 2 cut(s) 37, 1367
Eco57I CTGAAG 4 cut(s) 633, 855, 989, 1127
Eco88I CYCGRG 1 cut(s) 956
Eco91I GGTNACC 1 cut(s) 100
EcoO109I RGGNCCY 1 cut(s) 1364
EcoO65I GGTNACC 1 cut(s) 100
EcoT14I CCWWGG 5 cut(s) 863, 915, 1064, 1223, 1367
EcoT22I ATGCAT 1 cut(s) 824
EcoT38I GRGCYC 2 cut(s) 37, 1367
ErhI CCWWGG 5 cut(s) 863, 915, 1064, 1223, 1367
Esp3I CGTCTC 2 cut(s) 6, 181
FaeI CATG 8 cut(s) 46, 458, 556, 952, 1021, 1077, 1104, 1199
FalI AAGNNNNNCTT 2 cut(s) 591, 623
FaqI GGGAC 5 cut(s) 31, 60, 704, 1204, 1259
FatI CATG 8 cut(s) 42, 454, 552, 948, 1017, 1073, 1100, 1195
Fnu4HI GCNGC 7 cut(s) 26, 117, 120, 123, 1078, 1269, 1463
FokI GGATG 2 cut(s) 782, 1206
FriOI GRGCYC 2 cut(s) 37, 1367
Fsp4HI GCNGC 7 cut(s) 26, 117, 120, 123, 1078, 1269, 1463
FspBI CTAG 4 cut(s) 1007, 1031, 1331, 1471
GluI GCNGC 7 cut(s) 26, 117, 120, 123, 1078, 1269, 1463
HaeIII GGCC 3 cut(s) 125, 729, 1365
HapII CCGG 3 cut(s) 98, 230, 567
Hin1I GRCGYC 1 cut(s) 190
Hin1II CATG 8 cut(s) 46, 458, 556, 952, 1021, 1077, 1104, 1199
HincII GTYRAC 1 cut(s) 1383
HindII GTYRAC 1 cut(s) 1383
HindIII AAGCTT 1 cut(s) 737
HinfI GANTC 5 cut(s) 68, 73, 142, 291, 530
HpaI GTTAAC 1 cut(s) 1383
HpaII CCGG 3 cut(s) 98, 230, 567
HphI GGTGA 3 cut(s) 70, 112, 584
Hpy166II GTNNAC 5 cut(s) 443, 685, 761, 1340, 1383
Hpy8I GTNNAC 5 cut(s) 443, 685, 761, 1340, 1383
Hpy99I CGWCG 2 cut(s) 97, 195
HpyAV CCTTC 4 cut(s) 40, 106, 830, 1328
HpyCH4III ACNGT 4 cut(s) 337, 931, 1285, 1360
HpyCH4IV ACGT 4 cut(s) 190, 301, 625, 901
HpyCH4V TGCA 7 cut(s) 214, 353, 380, 623, 822, 1340, 1349
HpyF10VI GCNNNNNNNGC 2 cut(s) 122, 1346
HpyF3I CTNAG 5 cut(s) 234, 434, 465, 483, 1305
HpySE526I ACGT 4 cut(s) 190, 301, 625, 901
Hsp92I GRCGYC 1 cut(s) 190
Hsp92II CATG 8 cut(s) 46, 458, 556, 952, 1021, 1077, 1104, 1199
KpnI GGTACC 1 cut(s) 230
KspAI GTTAAC 1 cut(s) 1383
Kzo9I GATC 7 cut(s) 52, 175, 220, 1060, 1068, 1152, 1327
LmnI GCTCC 1 cut(s) 1010
Lsp1109I GCAGC 2 cut(s) 1255, 1449
LweI GCATC 4 cut(s) 12, 420, 861, 1017
MaeI CTAG 4 cut(s) 1007, 1031, 1331, 1471
MaeII ACGT 4 cut(s) 190, 301, 625, 901
MaeIII GTNAC 4 cut(s) 100, 572, 1002, 1157
MalI GATC 7 cut(s) 54, 177, 222, 1062, 1070, 1154, 1329
MboI GATC 7 cut(s) 52, 175, 220, 1060, 1068, 1152, 1327
MboII GAAGA 9 cut(s) 230, 257, 421, 430, 611, 626, 982, 1217, 1387
MfeI CAATTG 1 cut(s) 1090
MhlI GDGCHC 4 cut(s) 37, 199, 1342, 1367
MlsI TGGCCA 1 cut(s) 729
MluNI TGGCCA 1 cut(s) 729
MlyI GAGTC 2 cut(s) 82, 285
MmeI TCCRAC 2 cut(s) 913, 1270
Mox20I TGGCCA 1 cut(s) 729
Mph1103I ATGCAT 1 cut(s) 824
MscI TGGCCA 1 cut(s) 729
MseI TTAA 5 cut(s) 332, 806, 1212, 1382, 1437
MslI CAYNNNNRTG 1 cut(s) 678
Msp20I TGGCCA 1 cut(s) 729
MspI CCGG 3 cut(s) 98, 230, 567
MspR9I CCNGG 1 cut(s) 567
MunI CAATTG 1 cut(s) 1090
Mva1269I GAATGC 1 cut(s) 824
MwoI GCNNNNNNNGC 2 cut(s) 122, 1346
NciI CCSGG 1 cut(s) 567
NdeII GATC 7 cut(s) 52, 175, 220, 1060, 1068, 1152, 1327
NlaIII CATG 8 cut(s) 46, 458, 556, 952, 1021, 1077, 1104, 1199
NlaIV GGNNCC 4 cut(s) 228, 861, 954, 1365
NmeAIII GCCGAG 1 cut(s) 296
NmuCI GTSAC 4 cut(s) 100, 572, 1002, 1157
NsiI ATGCAT 1 cut(s) 824
NspI RCATGY 3 cut(s) 458, 1077, 1104
PciI ACATGT 1 cut(s) 454
PcsI WCGNNNNNNNCGW 1 cut(s) 907
PctI GAATGC 1 cut(s) 824
PfeI GAWTC 3 cut(s) 68, 142, 530
PkrI GCNGC 7 cut(s) 27, 118, 121, 124, 1079, 1270, 1464
PleI GAGTC 2 cut(s) 81, 285
PpsI GAGTC 2 cut(s) 81, 285
PscI ACATGT 1 cut(s) 454
PspEI GGTNACC 1 cut(s) 100
PspN4I GGNNCC 4 cut(s) 228, 861, 954, 1365
PspOMI GGGCCC 1 cut(s) 1363
PspPI GGNCC 2 cut(s) 1363, 1364
RsaI GTAC 3 cut(s) 228, 388, 1022
RsaNI GTAC 3 cut(s) 227, 387, 1021
RseI CAYNNNNRTG 1 cut(s) 678
SaqAI TTAA 5 cut(s) 332, 806, 1212, 1382, 1437
SatI GCNGC 7 cut(s) 26, 117, 120, 123, 1078, 1269, 1463
Sau3AI GATC 7 cut(s) 52, 175, 220, 1060, 1068, 1152, 1327
Sau96I GGNCC 2 cut(s) 1363, 1364
SchI GAGTC 2 cut(s) 82, 285
ScrFI CCNGG 1 cut(s) 567
SduI GDGCHC 4 cut(s) 37, 199, 1342, 1367
SfaNI GCATC 4 cut(s) 12, 420, 861, 1017
SfcI CTRYAG 2 cut(s) 170, 1281
SgrAI CRCCGGYG 1 cut(s) 97
SmiMI CAYNNNNRTG 1 cut(s) 678
SmlI CTYRAG 1 cut(s) 813
SmoI CTYRAG 1 cut(s) 813
SsiI CCGC 8 cut(s) 9, 25, 50, 116, 119, 122, 1078, 1372
SspMI CTAG 4 cut(s) 1007, 1031, 1331, 1471
StyD4I CCNGG 1 cut(s) 565
StyI CCWWGG 5 cut(s) 863, 915, 1064, 1223, 1367
TaaI ACNGT 4 cut(s) 337, 931, 1285, 1360
TaiI ACGT 4 cut(s) 193, 304, 628, 904
TaqI TCGA 5 cut(s) 66, 71, 193, 1314, 1388
TauI GCSGC 5 cut(s) 28, 119, 122, 125, 1080
TfiI GAWTC 3 cut(s) 68, 142, 530
Tru1I TTAA 5 cut(s) 332, 806, 1212, 1382, 1437
Tru9I TTAA 5 cut(s) 332, 806, 1212, 1382, 1437
TscAI CASTG 1 cut(s) 1365
TseFI GTSAC 4 cut(s) 100, 572, 1002, 1157
TseI GCWGC 2 cut(s) 1268, 1462
Tsp45I GTSAC 4 cut(s) 100, 572, 1002, 1157
TspDTI ATGAA 5 cut(s) 59, 422, 510, 1272, 1393
TspRI CASTG 1 cut(s) 1365
VneI GTGCAC 1 cut(s) 1338
XapI RAATTY 4 cut(s) 259, 472, 1128, 1309
XbaI TCTAGA 1 cut(s) 1330
XceI RCATGY 3 cut(s) 458, 1077, 1104
XspI CTAG 4 cut(s) 1007, 1031, 1331, 1471
ZraI GACGTC 1 cut(s) 191
Zsp2I ATGCAT 1 cut(s) 824
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.