MD15G1082200.v1.1

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0006308
Physical Location & Seq
Forward (+)
5633532 .. 5638247
4716 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1082200.v1.1.491

Sequence Viewer

Length: 1578 bp
ATGGACGGCGGCGATGGGACGATGAGGCTCGGTGCTCTTAACTTGAAGCCGGATCGGGTTGGCGGCTTCGAGTCGGGTCCCGACGTGTCGGTTTCGTCGCCGGTCACGCGCCAGAAGGCGGTGGCGGCAAAGCAGTTTATTGAGAATCACTACAAGAACTACCTTCAAGGTCTGCAGGATCGCAAAGAGAGACGTCGAGCGCTTCAAAGGAAAGTACAAGAAGATCAGATACCGAGTGAGGAACAAGAGGAGATGATGAGGAATTTGGAACGCCGAGAAACAGAATATATGAGGATGCAAAGACGTAAGATTGGCATTGATGATTTTGAGCAATTAACTGTGATTGGCAAAGGTGCATTTGGTGAGGTAAGGTTATGTCGTGCTAAAGGTACAGGAGAGATTTATGCCATGAAGAAACTGAAGAAATCAGAGATGCTTAGTCGCGGACAGGTTGAGCATGTGCGTTCTGAGAGGAACTTGCTTGCGGAGGTTGATAGTCGGTGCATTGTAAAACTTCATTATTCGTTTCAAGATTCTGATTTCTTGTACCTTATCATGGAGTACTTACCTGGTGGTGATATTATGACATTACTGATGAGAGAAGATACTCTTTCTGAAGATGTTGCTCGTTTCTACATGGCAGAGAGTATTCTGGCTATTCACTCAATCCATCAACACAACTACATTCATAGGGACATAAAACCAGATAACCTGATACTGGATAAAAATGGCCATTTGAAGCTTTCAGATTTTGGCTTGTGTAAACCTCTGGATGATAAGCATTCGACATTGTTGCTGGAAAATGATGATTTGACCTCCCAGGAATCCATATCTGAAACTGAAGGACAATCTGGTTGTGATAAGGTTCCTTGGCTGATGCCAAAAGAACAGTTACAACAATGGAAACGAAATCGCCGTGCCTTGGCTTATTCAACCGTTGGAACTCTTGACTATATGGCACCTGAGGTGTTGCTCAAAAAAGGATATGGAATAGAGTGTGATTGGTGGTCCCTAGGGGCAATCATGTATGAGATGCTTGTAGGCTATCCTCCCTTCTACTCTGATGACCCAAGGATCACATGCCGCAAGATAATCAATTGGAGGACATACCTGAAATTCCCAGACGAACCCAAAATCTCAGAAGAAGCAAAGGATCTGATCTGTCACTTGTTATGTGATGTTGAGTCAAGGCTTGGGACTCATGGAGCAGAAGAAATTAAGGCCCATCCATGGTTCAGATCTATTCAGTGGGACAGGCTGTATGAAGGCGAAGCTGCATATAAACCCAGAGTCATTGGAGACTTGGACACTCAGAATTTTGAGAAGTTTCCTGAAGTAGAGGGACCGCCATCTACAATGCCAACAGTGGGACCTTGGCGGAAGATGTTAACATCAAAAGATACCAATTTCATTGGATACACCTTTAAGAAATCCGATGTCCTTAAATCGCTTGAAAGTTCAGGTACAGATATGAGATCAAATGGATCTTCAAAGTCCCCTTCTCTGATTTCCTTGTTAGGTAGAATTGACCTGCAAGACACTGTGATACCGGAGGGCGATCCAAAGCAGGAAATCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000166 GO:0000902 GO:0000904 GO:0002009 GO:0002165 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005488 GO:0005524 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005938 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0007275 GO:0007399 GO:0007423 GO:0007444 GO:0007455 GO:0007469 GO:0007472 GO:0007476 GO:0007552 GO:0007560 GO:0008037 GO:0008038 GO:0008144 GO:0008150 GO:0008152 GO:0008544 GO:0009653 GO:0009791 GO:0009886 GO:0009887 GO:0009888 GO:0009913 GO:0009987 GO:0010769 GO:0010975 GO:0016043 GO:0016301 GO:0016310 GO:0016358 GO:0016740 GO:0016772 GO:0016773 GO:0017076 GO:0018105 GO:0018193 GO:0018209 GO:0019538 GO:0022008 GO:0022416 GO:0022603 GO:0022604 GO:0023052 GO:0030030 GO:0030154 GO:0030182 GO:0030424 GO:0030425 GO:0030554 GO:0030855 GO:0031175 GO:0031344 GO:0032501 GO:0032502 GO:0032553 GO:0032555 GO:0032559 GO:0032989 GO:0032990 GO:0035107 GO:0035114 GO:0035120 GO:0035214 GO:0035220 GO:0035239 GO:0035295 GO:0035315 GO:0035316 GO:0035317 GO:0035556 GO:0035639 GO:0036094 GO:0036211 GO:0036477 GO:0042995 GO:0043005 GO:0043167 GO:0043168 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044297 GO:0044424 GO:0044444 GO:0044463 GO:0044464 GO:0045595 GO:0045664 GO:0048468 GO:0048513 GO:0048563 GO:0048569 GO:0048666 GO:0048667 GO:0048699 GO:0048707 GO:0048729 GO:0048731 GO:0048736 GO:0048737 GO:0048800 GO:0048812 GO:0048813 GO:0048814 GO:0048856 GO:0048858 GO:0048869 GO:0050767 GO:0050773 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051128 GO:0051239 GO:0051716 GO:0051960 GO:0060284 GO:0060429 GO:0060562 GO:0065007 GO:0070451 GO:0070593 GO:0071704 GO:0071840 GO:0071944 GO:0097159 GO:0097367 GO:0097447 GO:0097458 GO:0099568 GO:0120025 GO:0120035 GO:0120036 GO:0120038 GO:0120039 GO:0140096 GO:1901265 GO:1901363 GO:1901564 GO:2000026
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase Pkinase_C

Protein Analysis

526

Amino Acids

60.31

Weight (kDa)

5.86

Isoelectric Point (pI)

49.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 109 - 262 3.7e-43 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 112 - 263 1.5e-21 Protein tyrosine and serine/threonine kinase
ABC1 PF03109 183 - 266 2.2e-07 ABC1 atypical kinase-like domain
Pkinase PF00069 309 - 412 2.6e-22 Protein kinase domain
Pkinase_C PF00433 433 - 475 1.8e-06 Protein kinase C terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 196
Acc36I ACCTGC 1 cut(s) 1539
AccB1I GGYRCC 1 cut(s) 958
AccII CGCG 2 cut(s) 109, 444
AciI CCGC 9 cut(s) 9, 63, 119, 125, 444, 485, 1084, 1346, 1378
AclWI GGATC 6 cut(s) 60, 186, 1082, 1161, 1492, 1553
AcoI YGGCCR 1 cut(s) 730
AcsI RAATTY 3 cut(s) 262, 1115, 1315
AcuI CTGAAG 4 cut(s) 440, 636, 861, 1353
AcyI GRCGYC 1 cut(s) 193
AfaI GTAC 5 cut(s) 216, 391, 548, 563, 1465
AfeI AGCGCT 1 cut(s) 201
AfiI CCNNNNNNNGG 6 cut(s) 118, 556, 718, 922, 1230, 1367
AflIII ACRYGT 1 cut(s) 84
AgsI TTSAA 8 cut(s) 46, 167, 206, 530, 739, 933, 1454, 1491
AjiI CACGTC 1 cut(s) 85
AjnI CCWGG 2 cut(s) 568, 819
AluBI AGCT 2 cut(s) 742, 1274
AluI AGCT 2 cut(s) 742, 1274
Alw21I GWGCWC 1 cut(s) 37
Alw26I GTCTC 2 cut(s) 184, 1293
AlwI GGATC 6 cut(s) 60, 186, 1082, 1161, 1492, 1553
Aor51HI AGCGCT 1 cut(s) 201
AoxI GGCC 2 cut(s) 730, 1221
ApeKI GCWGC 1 cut(s) 1274
ApoI RAATTY 3 cut(s) 262, 1115, 1315
AspA2I CCTAGG 1 cut(s) 1012
AspLEI GCGC 2 cut(s) 111, 202
AspS9I GGNCC 5 cut(s) 77, 1008, 1222, 1343, 1370
AsuHPI GGTGA 2 cut(s) 374, 587
AvaII GGWCC 4 cut(s) 77, 1008, 1343, 1370
AvrII CCTAGG 1 cut(s) 1012
AxyI CCTNAGG 1 cut(s) 963
BalI TGGCCA 1 cut(s) 732
BanI GGYRCC 1 cut(s) 958
BarI GAAGNNNNNNTAC 2 cut(s) 213, 245
Bbv12I GWGCWC 1 cut(s) 37
BbvI GCAGC 1 cut(s) 1261
BccI CCATC 4 cut(s) 8, 678, 1233, 1357
BceAI ACGGC 2 cut(s) 22, 900
BcgI CGANNNNNNTGC 2 cut(s) 775, 809
BciT130I CCWGG 2 cut(s) 570, 821
BciVI GTATCC 1 cut(s) 1409
BcoDI GTCTC 2 cut(s) 184, 1293
BfaI CTAG 1 cut(s) 1013
BfmI CTRYAG 1 cut(s) 173
BfoI RGCGCY 1 cut(s) 203
BfuAI ACCTGC 1 cut(s) 1539
BfuI GTATCC 1 cut(s) 1409
BglII AGATCT 1 cut(s) 1238
BisI GCNGC 5 cut(s) 10, 64, 126, 1084, 1275
BlnI CCTAGG 1 cut(s) 1012
BlsI GCNGC 5 cut(s) 11, 65, 127, 1085, 1276
BmcAI AGTACT 1 cut(s) 563
Bme1390I CCNGG 2 cut(s) 570, 821
Bme18I GGWCC 4 cut(s) 77, 1008, 1343, 1370
BmgBI CACGTC 1 cut(s) 85
BmgT120I GGNCC 5 cut(s) 77, 1008, 1222, 1343, 1370
BmiI GGNNCC 7 cut(s) 78, 79, 867, 960, 1010, 1344, 1371
BmrFI CCNGG 2 cut(s) 570, 821
BmsI GCATC 4 cut(s) 285, 423, 867, 1023
BsaHI GRCGYC 1 cut(s) 193
BsaJI CCNNGG 7 cut(s) 819, 869, 921, 1012, 1070, 1229, 1373
BsaWI WCCGGW 1 cut(s) 1549
Bsc4I CCNNNNNNNGG 6 cut(s) 118, 556, 718, 922, 1230, 1367
Bse118I RCCGGY 1 cut(s) 100
Bse1I ACTGG 1 cut(s) 723
Bse21I CCTNAGG 1 cut(s) 963
BseBI CCWGG 2 cut(s) 570, 821
BseDI CCNNGG 7 cut(s) 819, 869, 921, 1012, 1070, 1229, 1373
BseGI GGATG 3 cut(s) 300, 778, 1225
BseLI CCNNNNNNNGG 6 cut(s) 118, 556, 718, 922, 1230, 1367
BseMII CTCAG 4 cut(s) 459, 954, 1152, 1325
BseNI ACTGG 1 cut(s) 723
BseRI GAGGAG 1 cut(s) 263
BseXI GCAGC 1 cut(s) 1261
Bsh1236I CGCG 2 cut(s) 109, 444
BshFI GGCC 2 cut(s) 732, 1223
BshNI GGYRCC 1 cut(s) 958
BsiHKAI GWGCWC 1 cut(s) 37
BsiSI CCGG 3 cut(s) 50, 101, 1550
BslFI GGGAC 9 cut(s) 31, 63, 707, 994, 1210, 1265, 1356, 1383, 1480
BslI CCNNNNNNNGG 6 cut(s) 118, 556, 718, 922, 1230, 1367
BsmAI GTCTC 2 cut(s) 184, 1293
BsmBI CGTCTC 1 cut(s) 184
BsmFI GGGAC 9 cut(s) 31, 63, 707, 994, 1210, 1265, 1356, 1383, 1480
BsmI GAATGC 1 cut(s) 781
BsnI GGCC 2 cut(s) 732, 1223
Bsp1286I GDGCHC 1 cut(s) 37
Bsp19I CCATGG 1 cut(s) 1229
BspACI CCGC 9 cut(s) 9, 63, 119, 125, 444, 485, 1084, 1346, 1378
BspANI GGCC 2 cut(s) 732, 1223
BspCNI CTCAG 4 cut(s) 460, 955, 1151, 1324
BspFNI CGCG 2 cut(s) 109, 444
BspLI GGNNCC 7 cut(s) 78, 79, 867, 960, 1010, 1344, 1371
BspMAI CTGCAG 1 cut(s) 177
BspMI ACCTGC 1 cut(s) 1539
BspPI GGATC 6 cut(s) 60, 186, 1082, 1161, 1492, 1553
BspT107I GGYRCC 1 cut(s) 958
BsrFI RCCGGY 1 cut(s) 100
BsrI ACTGG 1 cut(s) 723
BssAI RCCGGY 1 cut(s) 100
BssECI CCNNGG 7 cut(s) 819, 869, 921, 1012, 1070, 1229, 1373
BssNI GRCGYC 1 cut(s) 193
BssT1I CCWWGG 6 cut(s) 869, 921, 1012, 1070, 1229, 1373
Bst2UI CCWGG 2 cut(s) 570, 821
Bst4CI ACNGT 5 cut(s) 340, 891, 937, 1366, 1543
BstACI GRCGYC 1 cut(s) 193
BstC8I GCNNGC 1 cut(s) 483
BstDEI CTNAG 5 cut(s) 437, 468, 963, 1138, 1311
BstDSI CCRYGG 1 cut(s) 1229
BstF5I GGATG 3 cut(s) 300, 778, 1225
BstFNI CGCG 2 cut(s) 109, 444
BstH2I RGCGCY 1 cut(s) 203
BstHHI GCGC 2 cut(s) 111, 202
BstMAI GTCTC 2 cut(s) 184, 1293
BstMWI GCNNNNNNNGC 2 cut(s) 106, 125
BstNI CCWGG 2 cut(s) 570, 821
BstNSI RCATGY 2 cut(s) 461, 1083
BstSCI CCNGG 2 cut(s) 568, 819
BstSFI CTRYAG 1 cut(s) 173
BstUI CGCG 2 cut(s) 109, 444
BstV1I GCAGC 1 cut(s) 1261
BstX2I RGATCY 3 cut(s) 1153, 1238, 1484
BstYI RGATCY 3 cut(s) 1153, 1238, 1484
Bsu36I CCTNAGG 1 cut(s) 963
BsuI GTATCC 1 cut(s) 1409
BsuRI GGCC 2 cut(s) 732, 1223
BtgI CCRYGG 1 cut(s) 1229
BtgZI GCGATG 1 cut(s) 27
BtrI CACGTC 1 cut(s) 85
BtsCI GGATG 3 cut(s) 300, 778, 1225
BtsIMutI CAGTG 3 cut(s) 1253, 1371, 1539
BveI ACCTGC 1 cut(s) 1539
Cac8I GCNNGC 1 cut(s) 483
CfoI GCGC 2 cut(s) 111, 202
Cfr10I RCCGGY 1 cut(s) 100
Cfr13I GGNCC 5 cut(s) 77, 1008, 1222, 1343, 1370
CsiI ACCWGGT 1 cut(s) 568
Csp6I GTAC 5 cut(s) 215, 390, 547, 562, 1464
CviAII CATG 8 cut(s) 409, 458, 556, 637, 1024, 1080, 1202, 1230
CviQI GTAC 5 cut(s) 215, 390, 547, 562, 1464
DdeI CTNAG 5 cut(s) 437, 468, 963, 1138, 1311
EaeI YGGCCR 1 cut(s) 730
EciI GGCGGA 1 cut(s) 1393
Eco130I CCWWGG 6 cut(s) 869, 921, 1012, 1070, 1229, 1373
Eco47I GGWCC 4 cut(s) 77, 1008, 1343, 1370
Eco47III AGCGCT 1 cut(s) 201
Eco57I CTGAAG 4 cut(s) 440, 636, 861, 1353
Eco81I CCTNAGG 1 cut(s) 963
EcoO109I RGGNCCY 2 cut(s) 77, 1370
EcoRII CCWGG 2 cut(s) 568, 819
EcoT14I CCWWGG 6 cut(s) 869, 921, 1012, 1070, 1229, 1373
ErhI CCWWGG 6 cut(s) 869, 921, 1012, 1070, 1229, 1373
Esp3I CGTCTC 1 cut(s) 184
FaeI CATG 8 cut(s) 412, 461, 559, 640, 1027, 1083, 1205, 1233
FalI AAGNNNNNCTT 2 cut(s) 594, 626
FaqI GGGAC 9 cut(s) 31, 63, 707, 994, 1210, 1265, 1356, 1383, 1480
FatI CATG 8 cut(s) 408, 457, 555, 636, 1023, 1079, 1201, 1229
Fnu4HI GCNGC 5 cut(s) 10, 64, 126, 1084, 1275
FokI GGATG 3 cut(s) 307, 785, 1212
Fsp4HI GCNGC 5 cut(s) 10, 64, 126, 1084, 1275
FspBI CTAG 1 cut(s) 1013
GlaI GCGC 2 cut(s) 110, 201
GluI GCNGC 5 cut(s) 10, 64, 126, 1084, 1275
HaeII RGCGCY 1 cut(s) 203
HaeIII GGCC 2 cut(s) 732, 1223
HapII CCGG 3 cut(s) 50, 101, 1550
HhaI GCGC 2 cut(s) 111, 202
Hin1I GRCGYC 1 cut(s) 193
Hin1II CATG 8 cut(s) 412, 461, 559, 640, 1027, 1083, 1205, 1233
Hin6I GCGC 2 cut(s) 109, 200
HinP1I GCGC 2 cut(s) 109, 200
HincII GTYRAC 1 cut(s) 1389
HindII GTYRAC 1 cut(s) 1389
HindIII AAGCTT 1 cut(s) 740
HinfI GANTC 7 cut(s) 71, 145, 533, 824, 1184, 1198, 1290
HpaI GTTAAC 1 cut(s) 1389
HpaII CCGG 3 cut(s) 50, 101, 1550
HphI GGTGA 2 cut(s) 374, 587
Hpy166II GTNNAC 2 cut(s) 764, 1389
Hpy188III TCNNGA 5 cut(s) 80, 530, 770, 947, 1331
Hpy8I GTNNAC 2 cut(s) 764, 1389
Hpy99I CGWCG 3 cut(s) 86, 100, 198
HpyAV CCTTC 6 cut(s) 109, 173, 836, 1063, 1259, 1509
HpyCH4III ACNGT 5 cut(s) 340, 891, 937, 1366, 1543
HpyCH4IV ACGT 3 cut(s) 84, 193, 304
HpyCH4V TGCA 6 cut(s) 175, 298, 356, 504, 1277, 1534
HpyF10VI GCNNNNNNNGC 2 cut(s) 106, 125
HpyF3I CTNAG 5 cut(s) 437, 468, 963, 1138, 1311
HpySE526I ACGT 3 cut(s) 84, 193, 304
Hsp92I GRCGYC 1 cut(s) 193
Hsp92II CATG 8 cut(s) 412, 461, 559, 640, 1027, 1083, 1205, 1233
HspAI GCGC 2 cut(s) 109, 200
KflI GGGWCCC 1 cut(s) 77
KspAI GTTAAC 1 cut(s) 1389
LmnI GCTCC 1 cut(s) 1205
Lsp1109I GCAGC 1 cut(s) 1261
LweI GCATC 4 cut(s) 285, 423, 867, 1023
MabI ACCWGGT 1 cut(s) 568
MaeI CTAG 1 cut(s) 1013
MaeII ACGT 3 cut(s) 84, 193, 304
MaeIII GTNAC 3 cut(s) 103, 891, 1163
MboII GAAGA 9 cut(s) 233, 424, 433, 614, 629, 1154, 1223, 1393, 1479
MfeI CAATTG 1 cut(s) 1096
MflI RGATCY 3 cut(s) 1153, 1238, 1484
MhlI GDGCHC 1 cut(s) 37
MlsI TGGCCA 1 cut(s) 732
MluCI AATT 8 cut(s) 262, 332, 1096, 1115, 1215, 1315, 1405, 1524
MluNI TGGCCA 1 cut(s) 732
MlyI GAGTC 4 cut(s) 80, 1192, 1193, 1299
MmeI TCCRAC 1 cut(s) 919
Mox20I TGGCCA 1 cut(s) 732
MscI TGGCCA 1 cut(s) 732
MseI TTAA 6 cut(s) 39, 335, 1218, 1388, 1425, 1443
Msp20I TGGCCA 1 cut(s) 732
MspI CCGG 3 cut(s) 50, 101, 1550
MspR9I CCNGG 2 cut(s) 570, 821
MunI CAATTG 1 cut(s) 1096
Mva1269I GAATGC 1 cut(s) 781
MvaI CCWGG 2 cut(s) 570, 821
MvnI CGCG 2 cut(s) 109, 444
MwoI GCNNNNNNNGC 2 cut(s) 106, 125
NcoI CCATGG 1 cut(s) 1229
NlaIII CATG 8 cut(s) 412, 461, 559, 640, 1027, 1083, 1205, 1233
NlaIV GGNNCC 7 cut(s) 78, 79, 867, 960, 1010, 1344, 1371
NmeAIII GCCGAG 1 cut(s) 299
NmuCI GTSAC 2 cut(s) 103, 1163
NspI RCATGY 2 cut(s) 461, 1083
PcsI WCGNNNNNNNCGW 1 cut(s) 913
PctI GAATGC 1 cut(s) 781
PfeI GAWTC 3 cut(s) 145, 533, 824
PkrI GCNGC 5 cut(s) 11, 65, 127, 1085, 1276
PleI GAGTC 4 cut(s) 79, 1192, 1192, 1298
PpsI GAGTC 4 cut(s) 79, 1192, 1192, 1298
PpuMI RGGWCCY 2 cut(s) 77, 1370
Psp5II RGGWCCY 2 cut(s) 77, 1370
Psp6I CCWGG 2 cut(s) 568, 819
PspGI CCWGG 2 cut(s) 568, 819
PspN4I GGNNCC 7 cut(s) 78, 79, 867, 960, 1010, 1344, 1371
PspPI GGNCC 5 cut(s) 77, 1008, 1222, 1343, 1370
PspPPI RGGWCCY 2 cut(s) 77, 1370
PstI CTGCAG 1 cut(s) 177
PsuI RGATCY 3 cut(s) 1153, 1238, 1484
RsaI GTAC 5 cut(s) 216, 391, 548, 563, 1465
RsaNI GTAC 5 cut(s) 215, 390, 547, 562, 1464
SaqAI TTAA 6 cut(s) 39, 335, 1218, 1388, 1425, 1443
SatI GCNGC 5 cut(s) 10, 64, 126, 1084, 1275
Sau96I GGNCC 5 cut(s) 77, 1008, 1222, 1343, 1370
ScaI AGTACT 1 cut(s) 563
SchI GAGTC 4 cut(s) 80, 1192, 1193, 1299
ScrFI CCNGG 2 cut(s) 570, 821
SduI GDGCHC 1 cut(s) 37
SexAI ACCWGGT 1 cut(s) 568
SfaNI GCATC 4 cut(s) 285, 423, 867, 1023
SfcI CTRYAG 1 cut(s) 173
SinI GGWCC 4 cut(s) 77, 1008, 1343, 1370
Sse9I AATT 8 cut(s) 262, 332, 1096, 1115, 1215, 1315, 1405, 1524
SsiI CCGC 9 cut(s) 9, 63, 119, 125, 444, 485, 1084, 1346, 1378
SspMI CTAG 1 cut(s) 1013
StyD4I CCNGG 2 cut(s) 568, 819
StyI CCWWGG 6 cut(s) 869, 921, 1012, 1070, 1229, 1373
TaaI ACNGT 5 cut(s) 340, 891, 937, 1366, 1543
TaiI ACGT 3 cut(s) 87, 196, 307
TaqI TCGA 3 cut(s) 69, 196, 785
TasI AATT 8 cut(s) 262, 332, 1096, 1115, 1215, 1315, 1405, 1524
TatI WGTACW 2 cut(s) 214, 561
TauI GCSGC 4 cut(s) 12, 66, 128, 1086
TfiI GAWTC 3 cut(s) 145, 533, 824
Tru1I TTAA 6 cut(s) 39, 335, 1218, 1388, 1425, 1443
Tru9I TTAA 6 cut(s) 39, 335, 1218, 1388, 1425, 1443
TscAI CASTG 3 cut(s) 1253, 1371, 1546
TseFI GTSAC 2 cut(s) 103, 1163
TseI GCWGC 1 cut(s) 1274
Tsp45I GTSAC 2 cut(s) 103, 1163
TspDTI ATGAA 5 cut(s) 425, 506, 677, 1278, 1399
TspRI CASTG 3 cut(s) 1253, 1371, 1546
VpaK11BI GGWCC 4 cut(s) 77, 1008, 1343, 1370
XapI RAATTY 3 cut(s) 262, 1115, 1315
XceI RCATGY 2 cut(s) 461, 1083
XmaJI CCTAGG 1 cut(s) 1012
XspI CTAG 1 cut(s) 1013
ZraI GACGTC 1 cut(s) 194
ZrmI AGTACT 1 cut(s) 563
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.