MD08G1103100.v1.1

Polysaccharide biosynthesis protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0012936
Physical Location & Seq
Reverse (-)
8731410 .. 8733020
1611 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1103100.v1.1.491

Sequence Viewer

Length: 1023 bp
ATGAAGATACTCGTCACCGGCGCCTCCGGTTACTTAGGCGGAAGATTATGCCACGCGCTACTGAAGCAAGGCCACTCCGTCCGCGCCCTCGTCCGCCCCACCAGCGACCTCTCCTCCCTTCCTCCACCGTCATCCACCGGTAACGCTTCCCTCGAGCTTGTCTACGGCGACGTCACTGACTACGAATCCCTCCTCTCCGCCTTCTCCAACTGCGACGTCGTTTTCCACGCCGCCGCCGTCGTGGAGCCCTGGCTTCCCGACCCCTCCAAATTCTTCTCCGTCAACGTCGGGGGAATGAAGAACGTGCTGCGAGCGGTCCGGGAGACGAAGACCGTACAGAAAATCATCTACACGTCGTCGTTTTTCGCGCTCGGATCAACCGACGGCGGCGTCGCTGACGAGACGCAGGTCCATCACGAGAAGTTCTTCTGCACGGAGTACGAGAGATCGAAAGCCGCCGCCGATAAAATTGCGCTGCGAGCCGCGCAGCAAGAGGAGCTGCCGTTGGTGCTGCTTTATCCCGGAGTCATCTACGGCCCCGGAAAACTCACAGCCGGCAACGTCGTCGCTCGGTTGATCGTCGAGCGGTTCAACGGCCGGTTGCCGGGGTACATTGGCTCCGGGAACGACAGGTATTCGTTTAGCCATGTGGACGACGTCGTAGAGGGCCATATCAGAGCAATGGCAAAAGGTCGAACAGGTGAGAGGTATCTGCTGACAGGCGAAAACGCATCGTTTAAGCACGTTTTCGATGTGGCGGCCGTGATCACACAAACCGGTAGGCCGAAATTTGGGATCCCATTGTGGCTGATCGAGGTGTACGGATGGGCGTCTGTTCTTTTCTCTCGGGTTACAGGGAAGCTTCCCCTGATTAGTCCTCCGACTGTTTATGTTCTGAGGCATCAGTGGGCGTATTCGTGCGAGAAGGCGAAGGCCGAGCTGGGTTACAGTCCTAGGGGATTGAAAGAAGGATTGGGGGAGGTGTTGCCATGGCTCAATAGCTTGGGTTTGATCAAATACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00900 ko00909 ko01130 map00900 map00909 map01130
Pfam Domains
Protein Families
Epimerase

Protein Analysis

341

Amino Acids

37.11

Weight (kDa)

8.87

Isoelectric Point (pI)

25.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RmlD_sub_bind PF04321 1 - 156 1.1e-06 RmlD substrate binding domain
NmrA PF05368 2 - 150 5.1e-17 NmrA-like family
Epimerase PF01370 3 - 237 1.5e-38 NAD dependent epimerase/dehydratase family
Polysacc_synt_2 PF02719 3 - 124 4.1e-10 Polysaccharide biosynthesis protein
GDP_Man_Dehyd PF16363 4 - 254 2e-23 GDP-mannose 4,6 dehydratase
3Beta_HSD PF01073 4 - 228 1.2e-22 3-beta hydroxysteroid dehydrogenase/isomerase family
NAD_binding_10 PF13460 7 - 176 8.3e-17 NAD(P)H-binding
NAD_binding_4 PF07993 68 - 222 2e-15 Male sterility protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012936)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G33360 AT4G33360
fragaria_vesca FvH4_2g34590
malus_domestica MD08G1103100.v1.1
prunus_persica Prupe.1G438100_v2.0.a1
pyrus_communis pycom08g08450
rosa_chinensis RchiOBHm_Chr4g0409851 RchiOBHm_Chr5g0013111 RchiOBHm_Chr6g0307561 RchiOBHm_Chr6g0307711
rosa_laevigata RLG00000010706
rosa_multiflora Rmu_sc0000031.1_g000026
rosa_rugosa Rorug06G0359800
rosa_samantha Rh6AG469200 Rh6BG479800 Rh6CG485400 Rh6DG472000
rosa_wichuraiana Rw6G040970

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 389
AatII GACGTC 3 cut(s) 174, 219, 660
Acc36I ACCTGC 1 cut(s) 397
AccB1I GGYRCC 1 cut(s) 20
AccBSI CCGCTC 2 cut(s) 314, 586
AccI GTMKAC 1 cut(s) 162
AccII CGCG 4 cut(s) 56, 84, 368, 485
AclWI GGATC 3 cut(s) 382, 790, 803
AcoI YGGCCR 2 cut(s) 595, 759
AcsI RAATTY 2 cut(s) 269, 788
AcuI CTGAAG 1 cut(s) 83
AcyI GRCGYC 6 cut(s) 21, 171, 216, 390, 657, 830
AfaI GTAC 4 cut(s) 336, 440, 611, 821
AfiI CCNNNNNNNGG 2 cut(s) 604, 791
AflIII ACRYGT 1 cut(s) 351
AgeI ACCGGT 2 cut(s) 137, 776
AgsI TTSAA 2 cut(s) 592, 964
AjiI CACGTC 1 cut(s) 354
AjnI CCWGG 1 cut(s) 248
AjuI GAANNNNNNNTTGG 2 cut(s) 956, 988
AluBI AGCT 5 cut(s) 157, 499, 862, 940, 1002
AluI AGCT 5 cut(s) 157, 499, 862, 940, 1002
Alw26I GTCTC 2 cut(s) 317, 395
AlwI GGATC 3 cut(s) 382, 790, 803
Ama87I CYCGRG 2 cut(s) 152, 846
AoxI GGCC 7 cut(s) 70, 535, 595, 667, 759, 782, 933
ApeKI GCWGC 5 cut(s) 307, 475, 487, 499, 511
ApoI RAATTY 2 cut(s) 269, 788
AsiGI ACCGGT 2 cut(s) 137, 776
Asp700I GAANNNNTTC 1 cut(s) 425
AspA2I CCTAGG 1 cut(s) 953
AspLEI GCGC 6 cut(s) 23, 58, 86, 370, 475, 487
AspS9I GGNCC 4 cut(s) 316, 409, 536, 667
AsuC2I CCSGG 5 cut(s) 320, 522, 540, 606, 622
AsuHPI GGTGA 2 cut(s) 7, 713
AvaI CYCGRG 2 cut(s) 152, 846
AvaII GGWCC 2 cut(s) 316, 409
AvrII CCTAGG 1 cut(s) 953
BamHI GGATCC 1 cut(s) 795
BanI GGYRCC 1 cut(s) 20
BanII GRGCYC 1 cut(s) 249
BauI CACGAG 1 cut(s) 416
BbsI GAAGAC 1 cut(s) 335
BbvI GCAGC 5 cut(s) 294, 462, 486, 498, 499
BccI CCATC 2 cut(s) 420, 819
BceAI ACGGC 7 cut(s) 181, 221, 400, 487, 550, 610, 746
BcgI CGANNNNNNTGC 4 cut(s) 452, 486, 547, 581
BciT130I CCWGG 1 cut(s) 250
BclI TGATCA 2 cut(s) 765, 1011
BcnI CCSGG 5 cut(s) 320, 522, 540, 606, 622
BcoDI GTCTC 2 cut(s) 317, 395
BfaI CTAG 1 cut(s) 954
BfoI RGCGCY 1 cut(s) 24
BfuAI ACCTGC 1 cut(s) 397
BlnI CCTAGG 1 cut(s) 953
Bme1390I CCNGG 6 cut(s) 250, 320, 522, 540, 606, 622
Bme18I GGWCC 2 cut(s) 316, 409
BmeT110I CYCGRG 2 cut(s) 152, 846
BmgBI CACGTC 1 cut(s) 354
BmgT120I GGNCC 4 cut(s) 316, 409, 536, 667
BmiI GGNNCC 5 cut(s) 22, 246, 538, 619, 797
BmrFI CCNGG 6 cut(s) 250, 320, 522, 540, 606, 622
BmsI GCATC 2 cut(s) 740, 910
BoxI GACNNNNGTC 1 cut(s) 407
BpiI GAAGAC 1 cut(s) 335
BpuMI CCSGG 5 cut(s) 320, 522, 540, 606, 622
BsaHI GRCGYC 6 cut(s) 21, 171, 216, 390, 657, 830
BsaJI CCNNGG 5 cut(s) 248, 538, 605, 953, 989
BsaWI WCCGGW 3 cut(s) 26, 137, 776
BsaXI ACNNNNNCTCC 6 cut(s) 98, 128, 488, 518, 602, 632
Bsc4I CCNNNNNNNGG 2 cut(s) 604, 791
Bse118I RCCGGY 5 cut(s) 17, 137, 554, 597, 776
Bse3DI GCAATG 1 cut(s) 687
BseBI CCWGG 1 cut(s) 250
BseDI CCNNGG 5 cut(s) 248, 538, 605, 953, 989
BseGI GGATG 2 cut(s) 131, 830
BseLI CCNNNNNNNGG 2 cut(s) 604, 791
BseMI GCAATG 1 cut(s) 687
BseMII CTCAG 1 cut(s) 887
BseRI GAGGAG 3 cut(s) 103, 182, 509
BseX3I CGGCCG 2 cut(s) 595, 759
BseXI GCAGC 5 cut(s) 294, 462, 486, 498, 499
BseYI CCCAGC 1 cut(s) 940
BsgI GTGCAG 1 cut(s) 415
Bsh1236I CGCG 4 cut(s) 56, 84, 368, 485
Bsh1285I CGRYCG 2 cut(s) 598, 762
BshFI GGCC 7 cut(s) 72, 537, 597, 669, 761, 784, 935
BshNI GGYRCC 1 cut(s) 20
BshTI ACCGGT 2 cut(s) 137, 776
BsiEI CGRYCG 2 cut(s) 598, 762
BsiHKCI CYCGRG 2 cut(s) 152, 846
BslI CCNNNNNNNGG 2 cut(s) 604, 791
BsmAI GTCTC 2 cut(s) 317, 395
BsmBI CGTCTC 2 cut(s) 317, 395
BsnI GGCC 7 cut(s) 72, 537, 597, 669, 761, 784, 935
BsoBI CYCGRG 2 cut(s) 152, 846
Bsp1286I GDGCHC 1 cut(s) 249
Bsp143I GATC 7 cut(s) 374, 446, 576, 765, 795, 810, 1011
Bsp19I CCATGG 1 cut(s) 989
BspANI GGCC 7 cut(s) 72, 537, 597, 669, 761, 784, 935
BspCNI CTCAG 1 cut(s) 888
BspFNI CGCG 4 cut(s) 56, 84, 368, 485
BspLI GGNNCC 5 cut(s) 22, 246, 538, 619, 797
BspMI ACCTGC 1 cut(s) 397
BspPI GGATC 3 cut(s) 382, 790, 803
BspT107I GGYRCC 1 cut(s) 20
BsrBI CCGCTC 2 cut(s) 314, 586
BsrDI GCAATG 1 cut(s) 687
BsrFI RCCGGY 5 cut(s) 17, 137, 554, 597, 776
BssAI RCCGGY 5 cut(s) 17, 137, 554, 597, 776
BssECI CCNNGG 5 cut(s) 248, 538, 605, 953, 989
BssMI GATC 7 cut(s) 374, 446, 576, 765, 795, 810, 1011
BssNI GRCGYC 6 cut(s) 21, 171, 216, 390, 657, 830
BssSI CACGAG 1 cut(s) 416
BssT1I CCWWGG 2 cut(s) 953, 989
Bst2BI CACGAG 1 cut(s) 416
Bst2UI CCWGG 1 cut(s) 250
Bst4CI ACNGT 4 cut(s) 129, 334, 886, 950
BstACI GRCGYC 6 cut(s) 21, 171, 216, 390, 657, 830
BstC8I GCNNGC 3 cut(s) 312, 480, 556
BstDEI CTNAG 2 cut(s) 34, 896
BstDSI CCRYGG 1 cut(s) 989
BstF5I GGATG 2 cut(s) 131, 830
BstFNI CGCG 4 cut(s) 56, 84, 368, 485
BstH2I RGCGCY 1 cut(s) 24
BstHHI GCGC 6 cut(s) 23, 58, 86, 370, 475, 487
BstKTI GATC 7 cut(s) 377, 449, 579, 768, 798, 813, 1014
BstMAI GTCTC 2 cut(s) 317, 395
BstMBI GATC 7 cut(s) 374, 446, 576, 765, 795, 810, 1011
BstMCI CGRYCG 2 cut(s) 598, 762
BstMWI GCNNNNNNNGC 6 cut(s) 64, 102, 479, 484, 496, 508
BstNI CCWGG 1 cut(s) 250
BstPAI GACNNNNGTC 1 cut(s) 407
BstSCI CCNGG 6 cut(s) 248, 318, 520, 538, 604, 620
BstUI CGCG 4 cut(s) 56, 84, 368, 485
BstV1I GCAGC 5 cut(s) 294, 462, 486, 498, 499
BstV2I GAAGAC 1 cut(s) 335
BstX2I RGATCY 1 cut(s) 795
BstYI RGATCY 1 cut(s) 795
BstZI CGGCCG 2 cut(s) 595, 759
BsuRI GGCC 7 cut(s) 72, 537, 597, 669, 761, 784, 935
BtgI CCRYGG 1 cut(s) 989
BtrI CACGTC 1 cut(s) 354
BtsCI GGATG 2 cut(s) 131, 830
BtsIMutI CAGTG 2 cut(s) 174, 911
BveI ACCTGC 1 cut(s) 397
Cac8I GCNNGC 3 cut(s) 312, 480, 556
CfoI GCGC 6 cut(s) 23, 58, 86, 370, 475, 487
Cfr10I RCCGGY 5 cut(s) 17, 137, 554, 597, 776
Cfr13I GGNCC 4 cut(s) 316, 409, 536, 667
CpoI CGGWCCG 1 cut(s) 316
CseI GACGC 3 cut(s) 379, 412, 819
Csp6I GTAC 4 cut(s) 335, 439, 610, 820
CspAI ACCGGT 2 cut(s) 137, 776
CspI CGGWCCG 1 cut(s) 316
CviAII CATG 2 cut(s) 647, 990
CviQI GTAC 4 cut(s) 335, 439, 610, 820
DdeI CTNAG 2 cut(s) 34, 896
DinI GGCGCC 1 cut(s) 22
DpnI GATC 7 cut(s) 376, 448, 578, 767, 797, 812, 1013
DpnII GATC 7 cut(s) 374, 446, 576, 765, 795, 810, 1011
DrdI GACNNNNNNGTC 1 cut(s) 389
DseDI GACNNNNNNGTC 1 cut(s) 389
EaeI YGGCCR 2 cut(s) 595, 759
EagI CGGCCG 2 cut(s) 595, 759
EciI GGCGGA 3 cut(s) 54, 83, 187
EclXI CGGCCG 2 cut(s) 595, 759
Eco130I CCWWGG 2 cut(s) 953, 989
Eco24I GRGCYC 1 cut(s) 249
Eco47I GGWCC 2 cut(s) 316, 409
Eco52I CGGCCG 2 cut(s) 595, 759
Eco57I CTGAAG 1 cut(s) 83
Eco88I CYCGRG 2 cut(s) 152, 846
EcoRII CCWGG 1 cut(s) 248
EcoT14I CCWWGG 2 cut(s) 953, 989
EcoT38I GRGCYC 1 cut(s) 249
EgeI GGCGCC 1 cut(s) 22
EheI GGCGCC 1 cut(s) 22
ErhI CCWWGG 2 cut(s) 953, 989
Esp3I CGTCTC 2 cut(s) 317, 395
FaeI CATG 2 cut(s) 650, 993
FaiI YATR 5 cut(s) 49, 648, 672, 891, 991
FatI CATG 2 cut(s) 646, 989
FbaI TGATCA 2 cut(s) 765, 1011
FblI GTMKAC 1 cut(s) 162
FokI GGATG 2 cut(s) 118, 837
FriOI GRGCYC 1 cut(s) 249
FspBI CTAG 1 cut(s) 954
GlaI GCGC 6 cut(s) 22, 57, 85, 369, 474, 486
GsaI CCCAGC 1 cut(s) 944
HaeII RGCGCY 1 cut(s) 24
HaeIII GGCC 7 cut(s) 72, 537, 597, 669, 761, 784, 935
HgaI GACGC 3 cut(s) 379, 412, 819
HhaI GCGC 6 cut(s) 23, 58, 86, 370, 475, 487
Hin1I GRCGYC 6 cut(s) 21, 171, 216, 390, 657, 830
Hin1II CATG 2 cut(s) 650, 993
Hin6I GCGC 6 cut(s) 21, 56, 84, 368, 473, 485
HinP1I GCGC 6 cut(s) 21, 56, 84, 368, 473, 485
HincII GTYRAC 1 cut(s) 283
HindII GTYRAC 1 cut(s) 283
HindIII AAGCTT 1 cut(s) 860
HinfI GANTC 2 cut(s) 185, 525
HphI GGTGA 2 cut(s) 7, 713
Hpy166II GTNNAC 4 cut(s) 163, 283, 652, 820
Hpy188I TCNGA 4 cut(s) 374, 677, 882, 897
Hpy188III TCNNGA 2 cut(s) 257, 416
Hpy8I GTNNAC 4 cut(s) 163, 283, 652, 820
HpyAV CCTTC 5 cut(s) 128, 211, 919, 925, 962
HpyCH4III ACNGT 4 cut(s) 129, 334, 886, 950
HpyCH4IV ACGT 8 cut(s) 171, 216, 285, 303, 353, 561, 657, 744
HpyCH4V TGCA 1 cut(s) 432
HpyF10VI GCNNNNNNNGC 6 cut(s) 64, 102, 479, 484, 496, 508
HpyF3I CTNAG 2 cut(s) 34, 896
HpySE526I ACGT 8 cut(s) 171, 216, 285, 303, 353, 561, 657, 744
Hsp92I GRCGYC 6 cut(s) 21, 171, 216, 390, 657, 830
Hsp92II CATG 2 cut(s) 650, 993
HspAI GCGC 6 cut(s) 21, 56, 84, 368, 473, 485
KasI GGCGCC 1 cut(s) 20
KroI GCCGGC 1 cut(s) 554
KroNI GCCGGC 1 cut(s) 556
Ksp22I TGATCA 2 cut(s) 765, 1011
Kzo9I GATC 7 cut(s) 374, 446, 576, 765, 795, 810, 1011
LmnI GCTCC 3 cut(s) 244, 496, 623
Lsp1109I GCAGC 5 cut(s) 294, 462, 486, 498, 499
LweI GCATC 2 cut(s) 740, 910
MaeI CTAG 1 cut(s) 954
MaeII ACGT 8 cut(s) 171, 216, 285, 303, 353, 561, 657, 744
MaeIII GTNAC 6 cut(s) 13, 29, 140, 172, 850, 944
MalI GATC 7 cut(s) 376, 448, 578, 767, 797, 812, 1013
MbiI CCGCTC 2 cut(s) 314, 586
MboI GATC 7 cut(s) 374, 446, 576, 765, 795, 810, 1011
MboII GAAGA 6 cut(s) 16, 54, 265, 310, 340, 418
MflI RGATCY 1 cut(s) 795
MhlI GDGCHC 1 cut(s) 249
MluCI AATT 3 cut(s) 269, 468, 788
Mly113I GGCGCC 1 cut(s) 21
MlyI GAGTC 1 cut(s) 534
MmeI TCCRAC 2 cut(s) 231, 905
MroNI GCCGGC 1 cut(s) 554
MroXI GAANNNNTTC 1 cut(s) 425
MseI TTAA 1 cut(s) 738
MspR9I CCNGG 6 cut(s) 250, 320, 522, 540, 606, 622
MvaI CCWGG 1 cut(s) 250
MvnI CGCG 4 cut(s) 56, 84, 368, 485
MwoI GCNNNNNNNGC 6 cut(s) 64, 102, 479, 484, 496, 508
NaeI GCCGGC 1 cut(s) 556
NarI GGCGCC 1 cut(s) 21
NciI CCSGG 5 cut(s) 320, 522, 540, 606, 622
NcoI CCATGG 1 cut(s) 989
NdeII GATC 7 cut(s) 374, 446, 576, 765, 795, 810, 1011
NgoMIV GCCGGC 1 cut(s) 554
NlaIII CATG 2 cut(s) 650, 993
NlaIV GGNNCC 5 cut(s) 22, 246, 538, 619, 797
NmeAIII GCCGAG 1 cut(s) 961
NmuCI GTSAC 2 cut(s) 13, 172
PaeR7I CTCGAG 1 cut(s) 152
PcsI WCGNNNNNNNCGW 3 cut(s) 150, 234, 378
PdiI GCCGGC 1 cut(s) 556
PdmI GAANNNNTTC 1 cut(s) 425
PfeI GAWTC 1 cut(s) 185
PflFI GACNNNGTC 1 cut(s) 656
PfoI TCCNGGA 3 cut(s) 318, 520, 620
PinAI ACCGGT 2 cut(s) 137, 776
PleI GAGTC 1 cut(s) 533
PluTI GGCGCC 1 cut(s) 24
PpsI GAGTC 1 cut(s) 533
PshAI GACNNNNGTC 1 cut(s) 407
Psp6I CCWGG 1 cut(s) 248
PspFI CCCAGC 1 cut(s) 940
PspGI CCWGG 1 cut(s) 248
PspN4I GGNNCC 5 cut(s) 22, 246, 538, 619, 797
PspPI GGNCC 4 cut(s) 316, 409, 536, 667
PspXI VCTCGAGB 1 cut(s) 152
PsuI RGATCY 1 cut(s) 795
PsyI GACNNNGTC 1 cut(s) 656
RsaI GTAC 4 cut(s) 336, 440, 611, 821
RsaNI GTAC 4 cut(s) 335, 439, 610, 820
Rsr2I CGGWCCG 1 cut(s) 316
RsrII CGGWCCG 1 cut(s) 316
SaqAI TTAA 1 cut(s) 738
Sau3AI GATC 7 cut(s) 374, 446, 576, 765, 795, 810, 1011
Sau96I GGNCC 4 cut(s) 316, 409, 536, 667
SchI GAGTC 1 cut(s) 534
ScrFI CCNGG 6 cut(s) 250, 320, 522, 540, 606, 622
SduI GDGCHC 1 cut(s) 249
SfaNI GCATC 2 cut(s) 740, 910
SfoI GGCGCC 1 cut(s) 22
Sfr274I CTCGAG 1 cut(s) 152
SgrAI CRCCGGYG 1 cut(s) 17
SinI GGWCC 2 cut(s) 316, 409
SlaI CTCGAG 1 cut(s) 152
SmlI CTYRAG 1 cut(s) 152
SmoI CTYRAG 1 cut(s) 152
Sse9I AATT 3 cut(s) 269, 468, 788
SspDI GGCGCC 1 cut(s) 20
SspMI CTAG 1 cut(s) 954
StyD4I CCNGG 6 cut(s) 248, 318, 520, 538, 604, 620
StyI CCWWGG 2 cut(s) 953, 989
TaaI ACNGT 4 cut(s) 129, 334, 886, 950
TaiI ACGT 8 cut(s) 174, 219, 288, 306, 356, 564, 660, 747
TaqI TCGA 6 cut(s) 153, 449, 582, 694, 750, 813
TasI AATT 3 cut(s) 269, 468, 788
TauI GCSGC 7 cut(s) 233, 236, 390, 458, 461, 485, 761
TfiI GAWTC 1 cut(s) 185
Tru1I TTAA 1 cut(s) 738
Tru9I TTAA 1 cut(s) 738
TscAI CASTG 2 cut(s) 181, 911
TseFI GTSAC 2 cut(s) 13, 172
TseI GCWGC 5 cut(s) 307, 475, 487, 499, 511
Tsp45I GTSAC 2 cut(s) 13, 172
TspDTI ATGAA 2 cut(s) 17, 311
TspGWI ACGGA 4 cut(s) 67, 268, 449, 837
TspRI CASTG 2 cut(s) 181, 911
Tth111I GACNNNGTC 1 cut(s) 656
VpaK11BI GGWCC 2 cut(s) 316, 409
XapI RAATTY 2 cut(s) 269, 788
XhoI CTCGAG 1 cut(s) 152
XmaJI CCTAGG 1 cut(s) 953
XmiI GTMKAC 1 cut(s) 162
XmnI GAANNNNTTC 1 cut(s) 425
XspI CTAG 1 cut(s) 954
ZraI GACGTC 3 cut(s) 172, 217, 658
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.