RchiOBHm_Chr6g0307561

Polysaccharide biosynthesis protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0012936
Physical Location & Seq
Forward (+)
65871340 .. 65873157
1818 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ27651

Sequence Viewer

Length: 1017 bp
ATGAAGGTAATTGTGACCGGCGCCTCCGGTTACTTAGGAGGAAGACTATGCCACAAGCTACTGGAACGGGGCCACTCCGTTCGGGCCTTGGTCCGGCCCAGCAGCGACCTCTCCTCCCTTCCTCCACCCTCCTCCGGTTCCCTTGAACTCGTTTACGGCGACGTCACTGACTACCACTCCCTCCTCTCCGCCATCTCCGGCTGCCACGTCGTTTTCCACGCCGCCGCCTTAGTCGAGCCCTGGCTTCCCGATCCCTCCGACTTCTTCTCCGTCAACGTGGGGGGATTGAAGAACGTGCTGAGAGCAATCAGGGAGACGAATACCGTAGAGAAACTCATCTACACGTCGTCGTCGTTTTTCGCTCTCGGACCCACCGACGGCGACGTCGCCGACGAGACTCAGTTCCATCACGAGAAATTCTTCTGCACGGAGTACGAGAAATCCAAAGCGGCCGCCGATAAGATCGCGTTACAAGCTGCGCAGCAAGAGGGTTTGCCGGTGCTTTTACTTTATCCGGGAGTAATTTACGGTCCCGGGAAACTCACCGCCGGCAATGTGGTTGCTCGGATGATCATCGAAAGGTTTAATGGACGATTGCCTGGGTATATAGGCGCCGGGACTGATAGGTATTCCTTTAGCCATGTCGATGATGTGGTAGATGGCCACATTGGAGCTTTGTATAAGGGGAGAATTGGGGAGAGATATCTGCTTACTGGCGAAAACGCATCGTTTAAGCATGTGTTTGATGTAGCTGCTGTACTGACTCATACGCAGAGGCCTAAGTTTAACATCCCATTGTGGTTGATTGAGGTCTATGGATGGGTGTCGATTCTCGGCTCTCGGGTTACAGGGAAGCTTCCTCTGATCAGCCCGCCGACTGTTTATGTTCTAAGGCATCAATGGGCGTATTCGTGTGAGAAGGCCAAGCAGGAGCTGGATTATAGTCCTAGAAGCTTGAAGGAAGGATTAGAGGAGGTGCTGCCCTGGCTCAAGAATTTGGGTTTGATCAAATACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00900 ko00909 ko01130 map00900 map00909 map01130
Pfam Domains
Protein Families
Epimerase

Protein Analysis

338

Amino Acids

37.2

Weight (kDa)

7.2

Isoelectric Point (pI)

31.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NmrA PF05368 2 - 116 3e-12 NmrA-like family
Epimerase PF01370 3 - 235 5.1e-35 NAD dependent epimerase/dehydratase family
Polysacc_synt_2 PF02719 3 - 117 1.2e-07 Polysaccharide biosynthesis protein
3Beta_HSD PF01073 4 - 224 1.4e-21 3-beta hydroxysteroid dehydrogenase/isomerase family
GDP_Man_Dehyd PF16363 4 - 252 1.5e-20 GDP-mannose 4,6 dehydratase
NAD_binding_10 PF13460 7 - 175 4.1e-16 NAD(P)H-binding
NAD_binding_4 PF07993 65 - 219 3.8e-14 Male sterility protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012936)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G33360 AT4G33360
fragaria_vesca FvH4_2g34590
malus_domestica MD08G1103100.v1.1
prunus_persica Prupe.1G438100_v2.0.a1
pyrus_communis pycom08g08450
rosa_chinensis RchiOBHm_Chr4g0409851 RchiOBHm_Chr5g0013111 RchiOBHm_Chr6g0307561 RchiOBHm_Chr6g0307711
rosa_laevigata RLG00000010706
rosa_multiflora Rmu_sc0000031.1_g000026
rosa_rugosa Rorug06G0359800
rosa_samantha Rh6AG469200 Rh6BG479800 Rh6CG485400 Rh6DG472000
rosa_wichuraiana Rw6G040970

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 383
AatII GACGTC 2 cut(s) 165, 387
Acc16I TGCGCA 1 cut(s) 480
AccB1I GGYRCC 2 cut(s) 20, 611
AccII CGCG 1 cut(s) 467
AciI CCGC 7 cut(s) 189, 222, 225, 449, 453, 546, 872
AclWI GGATC 1 cut(s) 245
AcoI YGGCCR 2 cut(s) 450, 661
AcsI RAATTY 2 cut(s) 416, 994
AcyI GRCGYC 4 cut(s) 21, 162, 384, 612
AfaI GTAC 2 cut(s) 434, 759
AfiI CCNNNNNNNGG 3 cut(s) 93, 134, 377
AflIII ACRYGT 1 cut(s) 342
AgsI TTSAA 3 cut(s) 146, 289, 958
AjiI CACGTC 2 cut(s) 208, 345
AjnI CCWGG 3 cut(s) 239, 598, 983
AluBI AGCT 7 cut(s) 58, 476, 674, 752, 856, 934, 954
AluI AGCT 7 cut(s) 58, 476, 674, 752, 856, 934, 954
Alw26I GTCTC 2 cut(s) 308, 389
AlwI GGATC 1 cut(s) 245
AlwNI CAGNNNCTG 1 cut(s) 934
Ama87I CYCGRG 2 cut(s) 533, 840
AoxI GGCC 7 cut(s) 70, 84, 95, 450, 661, 776, 921
ApeKI GCWGC 6 cut(s) 102, 201, 476, 481, 752, 979
ApoI RAATTY 2 cut(s) 416, 994
Asp700I GAANNNNTTC 1 cut(s) 419
AspLEI GCGC 3 cut(s) 23, 481, 614
AspS9I GGNCC 6 cut(s) 70, 84, 91, 96, 368, 530
AsuC2I CCSGG 4 cut(s) 516, 534, 535, 616
AsuHPI GGTGA 1 cut(s) 535
AvaI CYCGRG 2 cut(s) 533, 840
AvaII GGWCC 3 cut(s) 91, 368, 530
BalI TGGCCA 1 cut(s) 663
BanI GGYRCC 2 cut(s) 20, 611
BanII GRGCYC 1 cut(s) 240
BauI CACGAG 1 cut(s) 410
BbsI GAAGAC 1 cut(s) 49
BbvI GCAGC 6 cut(s) 114, 188, 463, 493, 739, 966
BccI CCATC 4 cut(s) 200, 414, 653, 813
BceAI ACGGC 2 cut(s) 172, 394
BciT130I CCWGG 3 cut(s) 241, 600, 985
BclI TGATCA 3 cut(s) 570, 864, 1005
BcnI CCSGG 4 cut(s) 516, 534, 535, 616
BcoDI GTCTC 2 cut(s) 308, 389
BfaI CTAG 1 cut(s) 948
BfoI RGCGCY 2 cut(s) 24, 615
Bme1390I CCNGG 7 cut(s) 241, 516, 534, 535, 600, 616, 985
Bme18I GGWCC 3 cut(s) 91, 368, 530
BmeT110I CYCGRG 2 cut(s) 533, 840
BmgBI CACGTC 2 cut(s) 208, 345
BmgT120I GGNCC 6 cut(s) 70, 84, 91, 96, 368, 530
BmiI GGNNCC 6 cut(s) 22, 71, 139, 370, 532, 613
BmrFI CCNGG 7 cut(s) 241, 516, 534, 535, 600, 616, 985
BmsI GCATC 2 cut(s) 734, 904
BpiI GAAGAC 1 cut(s) 49
BpuEI CTTGAG 1 cut(s) 974
BpuMI CCSGG 4 cut(s) 516, 534, 535, 616
BsaBI GATNNNNATC 1 cut(s) 572
BsaHI GRCGYC 4 cut(s) 21, 162, 384, 612
BsaJI CCNNGG 5 cut(s) 87, 239, 533, 599, 983
BsaWI WCCGGW 2 cut(s) 26, 134
BsaXI ACNNNNNCTCC 6 cut(s) 98, 128, 161, 191, 251, 281
Bsc4I CCNNNNNNNGG 3 cut(s) 93, 134, 377
Bse118I RCCGGY 3 cut(s) 17, 496, 548
Bse1I ACTGG 2 cut(s) 66, 718
Bse3DI GCAATG 1 cut(s) 559
Bse8I GATNNNNATC 1 cut(s) 572
BseBI CCWGG 3 cut(s) 241, 600, 985
BseDI CCNNGG 5 cut(s) 87, 239, 533, 599, 983
BseGI GGATG 3 cut(s) 573, 789, 824
BseJI GATNNNNATC 1 cut(s) 572
BseLI CCNNNNNNNGG 3 cut(s) 93, 134, 377
BseMI GCAATG 1 cut(s) 559
BseMII CTCAG 2 cut(s) 290, 413
BseNI ACTGG 2 cut(s) 66, 718
BseRI GAGGAG 4 cut(s) 103, 121, 173, 986
BseX3I CGGCCG 1 cut(s) 450
BseXI GCAGC 6 cut(s) 114, 188, 463, 493, 739, 966
BseYI CCCAGC 1 cut(s) 98
BsgI GTGCAG 1 cut(s) 409
Bsh1236I CGCG 1 cut(s) 467
Bsh1285I CGRYCG 1 cut(s) 453
BshFI GGCC 7 cut(s) 72, 86, 97, 452, 663, 778, 923
BshNI GGYRCC 2 cut(s) 20, 611
BsiEI CGRYCG 1 cut(s) 453
BsiHKCI CYCGRG 2 cut(s) 533, 840
BslFI GGGAC 2 cut(s) 516, 631
BslI CCNNNNNNNGG 3 cut(s) 93, 134, 377
BsmAI GTCTC 2 cut(s) 308, 389
BsmBI CGTCTC 1 cut(s) 308
BsmFI GGGAC 2 cut(s) 516, 631
BsnI GGCC 7 cut(s) 72, 86, 97, 452, 663, 778, 923
BsoBI CYCGRG 2 cut(s) 533, 840
Bsp1286I GDGCHC 1 cut(s) 240
Bsp143I GATC 5 cut(s) 250, 462, 570, 864, 1005
BspACI CCGC 7 cut(s) 189, 222, 225, 449, 453, 546, 872
BspANI GGCC 7 cut(s) 72, 86, 97, 452, 663, 778, 923
BspCNI CTCAG 2 cut(s) 291, 412
BspFNI CGCG 1 cut(s) 467
BspLI GGNNCC 6 cut(s) 22, 71, 139, 370, 532, 613
BspPI GGATC 1 cut(s) 245
BspT107I GGYRCC 2 cut(s) 20, 611
BsrDI GCAATG 1 cut(s) 559
BsrFI RCCGGY 3 cut(s) 17, 496, 548
BsrI ACTGG 2 cut(s) 66, 718
BssAI RCCGGY 3 cut(s) 17, 496, 548
BssECI CCNNGG 5 cut(s) 87, 239, 533, 599, 983
BssMI GATC 5 cut(s) 250, 462, 570, 864, 1005
BssNI GRCGYC 4 cut(s) 21, 162, 384, 612
BssSI CACGAG 1 cut(s) 410
BssT1I CCWWGG 1 cut(s) 87
Bst2BI CACGAG 1 cut(s) 410
Bst2UI CCWGG 3 cut(s) 241, 600, 985
Bst4CI ACNGT 3 cut(s) 325, 530, 880
BstACI GRCGYC 4 cut(s) 21, 162, 384, 612
BstC8I GCNNGC 2 cut(s) 550, 872
BstDEI CTNAG 6 cut(s) 34, 229, 299, 399, 780, 890
BstF5I GGATG 3 cut(s) 573, 789, 824
BstFNI CGCG 1 cut(s) 467
BstH2I RGCGCY 2 cut(s) 24, 615
BstHHI GCGC 3 cut(s) 23, 481, 614
BstKTI GATC 5 cut(s) 253, 465, 573, 867, 1008
BstMAI GTCTC 2 cut(s) 308, 389
BstMBI GATC 5 cut(s) 250, 462, 570, 864, 1005
BstMCI CGRYCG 1 cut(s) 453
BstMWI GCNNNNNNNGC 2 cut(s) 473, 985
BstNI CCWGG 3 cut(s) 241, 600, 985
BstNSI RCATGY 1 cut(s) 740
BstSCI CCNGG 7 cut(s) 239, 514, 532, 533, 598, 614, 983
BstUI CGCG 1 cut(s) 467
BstV1I GCAGC 6 cut(s) 114, 188, 463, 493, 739, 966
BstV2I GAAGAC 1 cut(s) 49
BstZI CGGCCG 1 cut(s) 450
BsuRI GGCC 7 cut(s) 72, 86, 97, 452, 663, 778, 923
BtrI CACGTC 2 cut(s) 208, 345
BtsCI GGATG 3 cut(s) 573, 789, 824
BtsIMutI CAGTG 1 cut(s) 165
Cac8I GCNNGC 2 cut(s) 550, 872
CaiI CAGNNNCTG 1 cut(s) 934
CciNI GCGGCCGC 1 cut(s) 450
CfoI GCGC 3 cut(s) 23, 481, 614
Cfr10I RCCGGY 3 cut(s) 17, 496, 548
Cfr13I GGNCC 6 cut(s) 70, 84, 91, 96, 368, 530
Cfr9I CCCGGG 1 cut(s) 533
Csp6I GTAC 2 cut(s) 433, 758
CviAII CATG 2 cut(s) 641, 737
CviQI GTAC 2 cut(s) 433, 758
DdeI CTNAG 6 cut(s) 34, 229, 299, 399, 780, 890
DinI GGCGCC 2 cut(s) 22, 613
DpnI GATC 5 cut(s) 252, 464, 572, 866, 1007
DpnII GATC 5 cut(s) 250, 462, 570, 864, 1005
DrdI GACNNNNNNGTC 1 cut(s) 383
DseDI GACNNNNNNGTC 1 cut(s) 383
EaeI YGGCCR 2 cut(s) 450, 661
EagI CGGCCG 1 cut(s) 450
EciI GGCGGA 1 cut(s) 178
EclXI CGGCCG 1 cut(s) 450
Eco130I CCWWGG 1 cut(s) 87
Eco147I AGGCCT 1 cut(s) 778
Eco24I GRGCYC 1 cut(s) 240
Eco32I GATATC 1 cut(s) 704
Eco47I GGWCC 3 cut(s) 91, 368, 530
Eco52I CGGCCG 1 cut(s) 450
Eco88I CYCGRG 2 cut(s) 533, 840
EcoRII CCWGG 3 cut(s) 239, 598, 983
EcoRV GATATC 1 cut(s) 704
EcoT14I CCWWGG 1 cut(s) 87
EcoT38I GRGCYC 1 cut(s) 240
EgeI GGCGCC 2 cut(s) 22, 613
EheI GGCGCC 2 cut(s) 22, 613
ErhI CCWWGG 1 cut(s) 87
Esp3I CGTCTC 1 cut(s) 308
FaeI CATG 2 cut(s) 644, 740
FaqI GGGAC 2 cut(s) 516, 631
FatI CATG 2 cut(s) 640, 736
FauI CCCGC 1 cut(s) 879
FbaI TGATCA 3 cut(s) 570, 864, 1005
FokI GGATG 3 cut(s) 580, 776, 831
FriOI GRGCYC 1 cut(s) 240
FspBI CTAG 1 cut(s) 948
FspI TGCGCA 1 cut(s) 480
GlaI GCGC 3 cut(s) 22, 480, 613
GsaI CCCAGC 1 cut(s) 102
HaeII RGCGCY 2 cut(s) 24, 615
HaeIII GGCC 7 cut(s) 72, 86, 97, 452, 663, 778, 923
HhaI GCGC 3 cut(s) 23, 481, 614
Hin1I GRCGYC 4 cut(s) 21, 162, 384, 612
Hin1II CATG 2 cut(s) 644, 740
Hin6I GCGC 3 cut(s) 21, 479, 612
HinP1I GCGC 3 cut(s) 21, 479, 612
HincII GTYRAC 1 cut(s) 274
HindII GTYRAC 1 cut(s) 274
HindIII AAGCTT 2 cut(s) 854, 952
HinfI GANTC 3 cut(s) 397, 763, 829
HphI GGTGA 1 cut(s) 535
Hpy166II GTNNAC 2 cut(s) 154, 274
Hpy188I TCNGA 4 cut(s) 259, 368, 567, 864
Hpy188III TCNNGA 3 cut(s) 248, 410, 991
Hpy8I GTNNAC 2 cut(s) 154, 274
Hpy99I CGWCG 9 cut(s) 164, 212, 349, 352, 355, 380, 386, 389, 395
HpyAV CCTTC 4 cut(s) 128, 913, 952, 956
HpyCH4III ACNGT 3 cut(s) 325, 530, 880
HpyCH4IV ACGT 6 cut(s) 162, 207, 276, 294, 344, 384
HpyCH4V TGCA 1 cut(s) 426
HpyF10VI GCNNNNNNNGC 2 cut(s) 473, 985
HpyF3I CTNAG 6 cut(s) 34, 229, 299, 399, 780, 890
HpySE526I ACGT 6 cut(s) 162, 207, 276, 294, 344, 384
Hsp92I GRCGYC 4 cut(s) 21, 162, 384, 612
Hsp92II CATG 2 cut(s) 644, 740
HspAI GCGC 3 cut(s) 21, 479, 612
KasI GGCGCC 2 cut(s) 20, 611
KroI GCCGGC 1 cut(s) 548
KroNI GCCGGC 1 cut(s) 550
Ksp22I TGATCA 3 cut(s) 570, 864, 1005
Kzo9I GATC 5 cut(s) 250, 462, 570, 864, 1005
LmnI GCTCC 2 cut(s) 671, 931
Lsp1109I GCAGC 6 cut(s) 114, 188, 463, 493, 739, 966
LweI GCATC 2 cut(s) 734, 904
MaeI CTAG 1 cut(s) 948
MaeII ACGT 6 cut(s) 162, 207, 276, 294, 344, 384
MaeIII GTNAC 5 cut(s) 13, 29, 163, 468, 844
MalI GATC 5 cut(s) 252, 464, 572, 866, 1007
MboI GATC 5 cut(s) 250, 462, 570, 864, 1005
MboII GAAGA 4 cut(s) 54, 256, 301, 412
MhlI GDGCHC 1 cut(s) 240
MlsI TGGCCA 1 cut(s) 663
MluCI AATT 5 cut(s) 9, 416, 522, 690, 994
MluNI TGGCCA 1 cut(s) 663
Mly113I GGCGCC 2 cut(s) 21, 612
MlyI GAGTC 2 cut(s) 391, 757
MmeI TCCRAC 1 cut(s) 282
Mox20I TGGCCA 1 cut(s) 663
MroNI GCCGGC 1 cut(s) 548
MroXI GAANNNNTTC 1 cut(s) 419
MscI TGGCCA 1 cut(s) 663
MseI TTAA 3 cut(s) 585, 732, 786
MslI CAYNNNNRTG 1 cut(s) 645
Msp20I TGGCCA 1 cut(s) 663
MspR9I CCNGG 7 cut(s) 241, 516, 534, 535, 600, 616, 985
MvaI CCWGG 3 cut(s) 241, 600, 985
MvnI CGCG 1 cut(s) 467
MwoI GCNNNNNNNGC 2 cut(s) 473, 985
NaeI GCCGGC 1 cut(s) 550
NarI GGCGCC 2 cut(s) 21, 612
NciI CCSGG 4 cut(s) 516, 534, 535, 616
NdeII GATC 5 cut(s) 250, 462, 570, 864, 1005
NgoMIV GCCGGC 1 cut(s) 548
NlaIII CATG 2 cut(s) 644, 740
NlaIV GGNNCC 6 cut(s) 22, 71, 139, 370, 532, 613
NmeAIII GCCGAG 1 cut(s) 813
NmuCI GTSAC 2 cut(s) 13, 163
NotI GCGGCCGC 1 cut(s) 450
NsbI TGCGCA 1 cut(s) 480
NspI RCATGY 1 cut(s) 740
PceI AGGCCT 1 cut(s) 778
PcsI WCGNNNNNNNCGW 4 cut(s) 156, 350, 372, 390
PdiI GCCGGC 1 cut(s) 550
PdmI GAANNNNTTC 1 cut(s) 419
PfeI GAWTC 1 cut(s) 829
PfoI TCCNGGA 1 cut(s) 514
PleI GAGTC 2 cut(s) 391, 757
PluTI GGCGCC 2 cut(s) 24, 615
PpsI GAGTC 2 cut(s) 391, 757
Psp6I CCWGG 3 cut(s) 239, 598, 983
PspFI CCCAGC 1 cut(s) 98
PspGI CCWGG 3 cut(s) 239, 598, 983
PspN4I GGNNCC 6 cut(s) 22, 71, 139, 370, 532, 613
PspPI GGNCC 6 cut(s) 70, 84, 91, 96, 368, 530
PstNI CAGNNNCTG 1 cut(s) 934
RsaI GTAC 2 cut(s) 434, 759
RsaNI GTAC 2 cut(s) 433, 758
RseI CAYNNNNRTG 1 cut(s) 645
SaqAI TTAA 3 cut(s) 585, 732, 786
Sau3AI GATC 5 cut(s) 250, 462, 570, 864, 1005
Sau96I GGNCC 6 cut(s) 70, 84, 91, 96, 368, 530
SchI GAGTC 2 cut(s) 391, 757
ScrFI CCNGG 7 cut(s) 241, 516, 534, 535, 600, 616, 985
SduI GDGCHC 1 cut(s) 240
SfaNI GCATC 2 cut(s) 734, 904
SfoI GGCGCC 2 cut(s) 22, 613
SinI GGWCC 3 cut(s) 91, 368, 530
SmaI CCCGGG 1 cut(s) 535
SmiMI CAYNNNNRTG 1 cut(s) 645
SmlI CTYRAG 1 cut(s) 989
SmoI CTYRAG 1 cut(s) 989
Sse9I AATT 5 cut(s) 9, 416, 522, 690, 994
SseBI AGGCCT 1 cut(s) 778
SsiI CCGC 7 cut(s) 189, 222, 225, 449, 453, 546, 872
SspDI GGCGCC 2 cut(s) 20, 611
SspMI CTAG 1 cut(s) 948
StuI AGGCCT 1 cut(s) 778
StyD4I CCNGG 7 cut(s) 239, 514, 532, 533, 598, 614, 983
StyI CCWWGG 1 cut(s) 87
TaaI ACNGT 3 cut(s) 325, 530, 880
TaiI ACGT 6 cut(s) 165, 210, 279, 297, 347, 387
TaqI TCGA 4 cut(s) 234, 576, 645, 827
TasI AATT 5 cut(s) 9, 416, 522, 690, 994
TatI WGTACW 1 cut(s) 757
TauI GCSGC 4 cut(s) 224, 227, 452, 455
TfiI GAWTC 1 cut(s) 829
Tru1I TTAA 3 cut(s) 585, 732, 786
Tru9I TTAA 3 cut(s) 585, 732, 786
TscAI CASTG 1 cut(s) 172
TseFI GTSAC 2 cut(s) 13, 163
TseI GCWGC 6 cut(s) 102, 201, 476, 481, 752, 979
Tsp45I GTSAC 2 cut(s) 13, 163
TspDTI ATGAA 1 cut(s) 17
TspGWI ACGGA 3 cut(s) 67, 259, 443
TspMI CCCGGG 1 cut(s) 533
TspRI CASTG 1 cut(s) 172
VpaK11BI GGWCC 3 cut(s) 91, 368, 530
XapI RAATTY 2 cut(s) 416, 994
XceI RCATGY 1 cut(s) 740
XcmI CCANNNNNNNNNTGG 1 cut(s) 931
XmaI CCCGGG 1 cut(s) 533
XmnI GAANNNNTTC 1 cut(s) 419
XspI CTAG 1 cut(s) 948
ZraI GACGTC 2 cut(s) 163, 385
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.