MD08G1112700.v1.1
NAC Family

Probably inactive leucine-rich repeat receptor-like protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0008421
Physical Location & Seq
Forward (+)
9996190 .. 10000061
3872 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1112700.v1.1.491

Sequence Viewer

Length: 2982 bp
ATGTGTGTGTTTTACGTGGATATTAATTCATTTGAGGTGATGATTAAGATGGAAAAGGGACAACCTCATCAAACACGTCTCTTATGGTGCATGCTCGTGTTCTTATTCTTCTTCTCAAATGATACGTGTGCATTGGATGGTATTGATGAGCTTCAGCTTCTCTTATCCTTCAAAGCTTATATCAACGACCCTTTACACTACCTCTCAAACTGGAACACTTCAGCCAACACTTTATGCAATTGGCATGGCATCACATGCAACGACAACAATAACATCAAAGCCATCGAGCTCCCTGGAAGAAACATCTCTGGCAAGATATCATCCTCCATCTTCCACTTGTCCCAAGTCGAAACCATCGACCTCGCCAACAATCAGCTATCCGGCCAAGTCCCCAAAGACGTGTTTATAAACGGGTCTAATTCGCTTCGCCATCTCAACTTCAGCAGCAACAACTTAACCGGCACAGTTCCACAAGGCTCACTCACAACCCTAGAGGTACTCGACTTGTCCAACAACATGATTTCAGGCAAAATCCCAAATGACATCGGATCGTTTTCGAGCTTGAAATTTCTTGACCTTGGAGGAAATTTGTTGGTGGGAAACATCCCTGGTTCCATATCAAACCTGTCTAGTTTGGAGTACTTAACTTTGGCCTCAAACCAACTTGTTGGAAGAATTCCTACACAATTAGGCCAAATGAAGAACTTGAAGTGGATCTACTTGGGCTACAACAATCTCTCCGGCAAAATCCCTGGACAAATTGGTAACCTTTTTGGCTTGAATCATCTCGATCTTGTTTACAACAACCTCACCGGCGAAATCCCATACACAATCAGCAACCTTTCTAATCTTCGGTACCTTTTCCTCTATGGAAACAAACTCACAGGTCCAGTTCCACAATCCTTATTTGGTCTCGAGAAACTCGTTTCTCTAGACCTTAGTGACAATTTTCTTTCGGGAGAGATACCGGAAAGTGTTTTCCAACTCCAAAACTTGGAAATCCTCCACCTTTTTTCAAACAACTTCACAGGAAAAATTCCCAACGCTTTGGCTTCTCTGCCAAGGCTTCAAGTCCTTCAGTTATGGTCAAACAAGTTCTCGGGCCAAATCCCGAAACGTCTCGGAAACCAAAACAACCTCACTGTCTTAGACCTTTCCTCAAACAACCTTTCTGGAAAAATACCTGATACGCTATGCGACTCAGGCCGGCTTTTTAAGCTCATCCTCTTCTCCAATTCACTCGAAGGCGAAATCCCCCGAAGTTTGTCTTTTTGCAAGAGCTTGAGCCGAGTAAGACTGCAGAACAACCGGCTTTCCGGTGAAATATTGGCCGAGTTCACCAAACTCCCACTTGTCTATTTCCTGGATATCTCGGGCAACAATCTCTCTGGCAGAATCGATGACAAAAAGTGGGACATGCCCTCTCTTCAAATGCTGAATATGGCGAGAAATCGATTTTTCGGGAAGTTACCGGAAAATTTCGGAAGCGAAAAGCTCGAGAACTTGGACTTGTCCGAAAATTGGTTATCGGGAACTATCTCACTGAGTTTTCGGAACTTGTCGGAACTAATGCAGTTGAAGCTTTCTCGCAATGAACTCTCTGGTCCCATCCCTCAACAATTGTCTTCATGCAAAAAGCTCGTGAGCCTCGACCTCAGCCACAACCGCCTCACCGGCACGATTCCCACTAGCCTCTCTGATATGCCGGTTCTCGGGGACCTAGATTTGTCCGAAAACCAAATATCGGGCGAGATTCCACGGAATTTGGGAGCTATAGTATCGCTTGTTCAAGTCAACATTTCCCACAACAAACTCCACGGCATGTTGCCGCCCACAGCAGCGTTTCTTGCCATAAATGCCAGCGCAGTGGACGGCAACAACCTCTGCGGCGGAAGTGACACCATTAGTGGTCTACCTCCGTGCAAGGGCGTTAAGAGAAACCCTACTTGGTGGTTTATTGTCACTTGTTTTCTTGTTGCATTATTGGCTTTTGGAGTCGCCGGTTATTTATTTGTGTTAATGAGGAGGAGGAGAAAGGATTTGGAGGTTAAAACGGTGGAGAGCAAAGATGGGATTTGGGAGCTGCAGTTTTTTGAATCGAGGGTTTCTAGATCGGTTACAATTCATGAAATTTTATCGGCAGCCAAAGAAGGTAATATCATTGCAATGGAAAAGACTGGGATTTTATATAAAGGAGAATCAGTTTCCAATGGAATGCAATTCCTGGTAAAAGAGGATTCTGTGAAAGCAATTCCACCAAGTTTATTGTCACAGATTGTTGCACTTGGGAAGCTTAGGCACCCCAATGTGATCAAACTGATCGGAATATGTCATTCCGAAAACGGTGCGTATGTGCTCTATGAGTATTGTGAAGGGAAAGTGTTGGGTCAAGTTATGAGGGATTTGAGTTGGGATCAGCGCCGGAAAATTGCGGTTGGAATTGCGAAGGCTCTACGGTTCTTGCACTGTTGTTGCTCGCCGAGCGTTGTAGCTGGTTGTGTGTCGGCAGAGAAAGTGATTGTGGACGCAAAAGATGAGCCTCGAATTCGATTGAGTCTCTCCGAACAAGTTCGGACGGACTCCAAGGGATTCGTTGTTTCAGCATACATTGCTCCGGATAAAGAAAGCAAAGCCGGCATTACAGAGAAGACTGACATCTATGGGTTTGGGCTCGTATTGATCGAATTGCTGACCGGAAAAGGCCCCAGTGACACTGAATTTGGCGCGCACCAGAGCGTCGTCGAGTGGGCTCGGTACTGCTACTCAGACTGCCATCTTGATGTCTGGACCGACTCGATGATCAGAGAACACGTGTCGAGCAATCAGAACGAGATTGTGGAAACCATGAACCTAGCCCTTCACTGCACTGCTAGTGACCCCATGGCTAGACCATGCGCGAATGAAATATATAAAACCCTAGAATCTGTAATGAGGACAAGTAGTTCTTGTGTTCCTGGATTAAAAGTTACTTCACCTTTTTGA

Protein Analysis

994

Amino Acids

109.76

Weight (kDa)

6.81

Isoelectric Point (pI)

30.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 49 - 87 5.2e-11 Leucine rich repeat N-terminal domain
LRR_14 PF23598 142 - 267 5.6e-10 Leucine-rich repeat region
LRR_8 PF13855 211 - 270 2e-07 Leucine rich repeat
LRR_8 PF13855 259 - 316 7.9e-09 Leucine rich repeat
LRR_14 PF23598 278 - 401 2e-13 Leucine-rich repeat region
LRR_8 PF13855 354 - 414 1.1e-06 Leucine rich repeat
LRR_8 PF13855 498 - 557 1.4e-08 Leucine rich repeat
LRR_8 PF13855 546 - 605 4.7e-08 Leucine rich repeat
PK_Tyr_Ser-Thr PF07714 741 - 973 4e-17 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 744 - 971 1.8e-17 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 407
Acc65I GGTACC 1 cut(s) 855
AccB1I GGYRCC 2 cut(s) 855, 2298
AccB7I CCANNNNNTGG 1 cut(s) 994
AccI GTMKAC 1 cut(s) 1912
AccII CGCG 2 cut(s) 2726, 2897
AccIII TCCGGA 1 cut(s) 2614
AciI CCGC 5 cut(s) 1666, 1829, 1887, 1890, 2432
AclWI GGATC 3 cut(s) 556, 722, 2421
AcoI YGGCCR 2 cut(s) 382, 1329
AcsI RAATTY 9 cut(s) 566, 586, 675, 1035, 1477, 1762, 2130, 2544, 2717
AcuI CTGAAG 4 cut(s) 137, 204, 424, 1061
AcvI CACGTG 1 cut(s) 2812
AfaI GTAC 4 cut(s) 498, 641, 857, 2756
AfiI CCNNNNNNNGG 5 cut(s) 994, 1521, 1712, 1744, 1925
AflIII ACRYGT 5 cut(s) 74, 125, 399, 2809, 2811
AjiI CACGTC 2 cut(s) 77, 400
AjnI CCWGG 6 cut(s) 292, 607, 751, 1362, 2223, 2953
AjuI GAANNNNNNNTTGG 2 cut(s) 1930, 1962
AloI GAACNNNNNNTCC 2 cut(s) 2926, 2958
Alw21I GWGCWC 2 cut(s) 291, 2358
Alw26I GTCTC 4 cut(s) 83, 917, 1124, 2561
AlwI GGATC 3 cut(s) 556, 722, 2421
Ama87I CYCGRG 5 cut(s) 914, 1099, 1372, 1496, 1712
Aor13HI TCCGGA 1 cut(s) 2614
AoxI GGCC 7 cut(s) 382, 651, 691, 1102, 1204, 1329, 2701
ApeKI GCWGC 4 cut(s) 444, 1838, 2083, 2141
ApoI RAATTY 9 cut(s) 566, 586, 675, 1035, 1477, 1762, 2130, 2544, 2717
ArsI GACNNNNNNTTYG 2 cut(s) 2702, 2734
AseI ATTAAT 1 cut(s) 24
Asp700I GAANNNNTTC 1 cut(s) 2568
Asp718I GGTACC 1 cut(s) 855
AspLEI GCGC 5 cut(s) 1865, 2421, 2726, 2728, 2897
AspS9I GGNCC 6 cut(s) 887, 1102, 1604, 1717, 2702, 2787
AsuHPI GGTGA 6 cut(s) 49, 802, 1330, 1331, 1663, 2964
AvaI CYCGRG 5 cut(s) 914, 1099, 1372, 1496, 1712
AvaII GGWCC 4 cut(s) 887, 1604, 1717, 2787
BanI GGYRCC 2 cut(s) 855, 2298
BanII GRGCYC 3 cut(s) 291, 2673, 2752
BarI GAAGNNNNNNTAC 4 cut(s) 664, 696, 701, 733
BauI CACGAG 2 cut(s) 95, 1640
BbrPI CACGTG 1 cut(s) 2812
BbsI GAAGAC 2 cut(s) 1617, 2654
Bbv12I GWGCWC 2 cut(s) 291, 2358
BbvCI CCTCAGC 1 cut(s) 1655
BbvI GCAGC 4 cut(s) 456, 1850, 2070, 2153
BccI CCATC 9 cut(s) 43, 131, 290, 335, 362, 438, 1616, 2063, 2781
BceAI ACGGC 2 cut(s) 1834, 1888
BcgI CGANNNNNNTGC 4 cut(s) 1621, 1655, 2327, 2361
BciT130I CCWGG 6 cut(s) 294, 609, 753, 1364, 2225, 2955
BclI TGATCA 2 cut(s) 2310, 2799
BcoDI GTCTC 4 cut(s) 83, 917, 1124, 2561
BfmI CTRYAG 3 cut(s) 1298, 1773, 2084
BfoI RGCGCY 1 cut(s) 2422
BglI GCCNNNNNGGC 1 cut(s) 1674
BisI GCNGC 6 cut(s) 445, 1829, 1839, 1888, 2084, 2142
BlsI GCNGC 6 cut(s) 446, 1830, 1840, 1889, 2085, 2143
BmcAI AGTACT 1 cut(s) 641
Bme1390I CCNGG 6 cut(s) 294, 609, 753, 1364, 2225, 2955
Bme18I GGWCC 4 cut(s) 887, 1604, 1717, 2787
BmeT110I CYCGRG 5 cut(s) 914, 1099, 1372, 1496, 1712
BmgBI CACGTC 2 cut(s) 77, 400
BmgT120I GGNCC 6 cut(s) 887, 1102, 1604, 1717, 2702, 2787
BmiI GGNNCC 6 cut(s) 613, 857, 1606, 1718, 2300, 2704
BmrFI CCNGG 6 cut(s) 294, 609, 753, 1364, 2225, 2955
BmrI ACTGGG 2 cut(s) 2187, 2700
BmsI GCATC 1 cut(s) 258
BmuI ACTGGG 2 cut(s) 2187, 2700
BpiI GAAGAC 2 cut(s) 1617, 2654
Bpu10I CCTNAGC 2 cut(s) 1655, 2294
BpuEI CTTGAG 1 cut(s) 1303
Bsa29I ATCGAT 2 cut(s) 1398, 1453
BsaAI YACGTR 3 cut(s) 16, 126, 2812
BsaI GGTCTC 1 cut(s) 917
BsaJI CCNNGG 9 cut(s) 292, 577, 607, 751, 1061, 1757, 1816, 2583, 2880
BsaWI WCCGGW 5 cut(s) 967, 1316, 1471, 2614, 2693
BsaXI ACNNNNNCTCC 8 cut(s) 722, 752, 1761, 1791, 2072, 2102, 2187, 2217
Bsc4I CCNNNNNNNGG 5 cut(s) 994, 1521, 1712, 1744, 1925
Bse118I RCCGGY 8 cut(s) 458, 812, 1206, 1308, 1673, 1705, 2000, 2633
Bse1I ACTGG 4 cut(s) 215, 890, 2182, 2706
Bse3DI GCAATG 4 cut(s) 1597, 2160, 2172, 2607
BseAI TCCGGA 1 cut(s) 2614
BseBI CCWGG 6 cut(s) 294, 609, 753, 1364, 2225, 2955
BseCI ATCGAT 2 cut(s) 1398, 1453
BseDI CCNNGG 9 cut(s) 292, 577, 607, 751, 1061, 1757, 1816, 2583, 2880
BseGI GGATG 5 cut(s) 142, 320, 603, 1221, 1608
BseLI CCNNNNNNNGG 5 cut(s) 994, 1521, 1712, 1744, 1925
BseMI GCAATG 4 cut(s) 1597, 2160, 2172, 2607
BseMII CTCAG 4 cut(s) 1215, 1535, 1669, 2778
BseNI ACTGG 4 cut(s) 215, 890, 2182, 2706
BsePI GCGCGC 1 cut(s) 2724
BseRI GAGGAG 3 cut(s) 2038, 2041, 2044
BseXI GCAGC 4 cut(s) 456, 1850, 2070, 2153
BsgI GTGCAG 1 cut(s) 2848
Bsh1236I CGCG 2 cut(s) 2726, 2897
BshFI GGCC 7 cut(s) 384, 653, 693, 1104, 1206, 1331, 2703
BshNI GGYRCC 2 cut(s) 855, 2298
BshVI ATCGAT 2 cut(s) 1398, 1453
BsiHKAI GWGCWC 2 cut(s) 291, 2358
BsiHKCI CYCGRG 5 cut(s) 914, 1099, 1372, 1496, 1712
BslFI GGGAC 6 cut(s) 72, 325, 374, 1427, 1590, 1730
BslI CCNNNNNNNGG 5 cut(s) 994, 1521, 1712, 1744, 1925
BsmAI GTCTC 4 cut(s) 83, 917, 1124, 2561
BsmBI CGTCTC 2 cut(s) 83, 1124
BsmFI GGGAC 6 cut(s) 72, 325, 374, 1427, 1590, 1730
BsmI GAATGC 1 cut(s) 2220
BsnI GGCC 7 cut(s) 384, 653, 693, 1104, 1206, 1331, 2703
Bso31I GGTCTC 1 cut(s) 917
BsoBI CYCGRG 5 cut(s) 914, 1099, 1372, 1496, 1712
Bsp1286I GDGCHC 4 cut(s) 291, 2358, 2673, 2752
Bsp13I TCCGGA 1 cut(s) 2614
Bsp143I GATC 9 cut(s) 548, 714, 790, 2111, 2310, 2319, 2413, 2679, 2799
Bsp19I CCATGG 1 cut(s) 2880
BspACI CCGC 5 cut(s) 1666, 1829, 1887, 1890, 2432
BspANI GGCC 7 cut(s) 384, 653, 693, 1104, 1206, 1331, 2703
BspCNI CTCAG 4 cut(s) 1214, 1536, 1668, 2777
BspDI ATCGAT 2 cut(s) 1398, 1453
BspEI TCCGGA 1 cut(s) 2614
BspFNI CGCG 2 cut(s) 2726, 2897
BspHI TCATGA 1 cut(s) 2125
BspLI GGNNCC 6 cut(s) 613, 857, 1606, 1718, 2300, 2704
BspMAI CTGCAG 2 cut(s) 1302, 2088
BspPI GGATC 3 cut(s) 556, 722, 2421
BspT107I GGYRCC 2 cut(s) 855, 2298
BspTNI GGTCTC 1 cut(s) 917
BsrDI GCAATG 4 cut(s) 1597, 2160, 2172, 2607
BsrFI RCCGGY 8 cut(s) 458, 812, 1206, 1308, 1673, 1705, 2000, 2633
BsrI ACTGG 4 cut(s) 215, 890, 2182, 2706
BssAI RCCGGY 8 cut(s) 458, 812, 1206, 1308, 1673, 1705, 2000, 2633
BssECI CCNNGG 9 cut(s) 292, 577, 607, 751, 1061, 1757, 1816, 2583, 2880
BssHII GCGCGC 1 cut(s) 2724
BssMI GATC 9 cut(s) 548, 714, 790, 2111, 2310, 2319, 2413, 2679, 2799
BssSI CACGAG 2 cut(s) 95, 1640
BssT1I CCWWGG 4 cut(s) 577, 1061, 2583, 2880
Bst2BI CACGAG 2 cut(s) 95, 1640
Bst2UI CCWGG 6 cut(s) 294, 609, 753, 1364, 2225, 2955
Bst4CI ACNGT 6 cut(s) 466, 1144, 2056, 2345, 2457, 2468
Bst6I CTCTTC 2 cut(s) 1232, 1431
BstAPI GCANNNNNTGC 2 cut(s) 255, 2609
BstBAI YACGTR 3 cut(s) 16, 126, 2812
BstC8I GCNNGC 6 cut(s) 92, 1208, 1861, 2477, 2635, 2726
BstDEI CTNAG 7 cut(s) 938, 1147, 1201, 1544, 1655, 2294, 2764
BstDSI CCRYGG 3 cut(s) 1757, 1816, 2880
BstEII GGTNACC 1 cut(s) 764
BstF5I GGATG 5 cut(s) 142, 320, 603, 1221, 1608
BstFNI CGCG 2 cut(s) 2726, 2897
BstH2I RGCGCY 1 cut(s) 2422
BstHHI GCGC 5 cut(s) 1865, 2421, 2726, 2728, 2897
BstKTI GATC 9 cut(s) 551, 717, 793, 2114, 2313, 2322, 2416, 2682, 2802
BstMAI GTCTC 4 cut(s) 83, 917, 1124, 2561
BstMBI GATC 9 cut(s) 548, 714, 790, 2111, 2310, 2319, 2413, 2679, 2799
BstNI CCWGG 6 cut(s) 294, 609, 753, 1364, 2225, 2955
BstNSI RCATGY 4 cut(s) 94, 258, 1420, 1825
BstPI GGTNACC 1 cut(s) 764
BstSCI CCNGG 6 cut(s) 292, 607, 751, 1362, 2223, 2953
BstSFI CTRYAG 3 cut(s) 1298, 1773, 2084
BstUI CGCG 2 cut(s) 2726, 2897
BstV1I GCAGC 4 cut(s) 456, 1850, 2070, 2153
BstV2I GAAGAC 2 cut(s) 1617, 2654
BstX2I RGATCY 1 cut(s) 714
BstXI CCANNNNNNTGG 3 cut(s) 668, 1048, 1867
BstYI RGATCY 1 cut(s) 714
Bsu15I ATCGAT 2 cut(s) 1398, 1453
BsuRI GGCC 7 cut(s) 384, 653, 693, 1104, 1206, 1331, 2703
BsuTUI ATCGAT 2 cut(s) 1398, 1453
BtgI CCRYGG 3 cut(s) 1757, 1816, 2880
BtrI CACGTC 2 cut(s) 77, 400
BtsCI GGATG 5 cut(s) 142, 320, 603, 1221, 1608
BtsI GCAGTG 3 cut(s) 1872, 2860, 2865
BtsIMutI CAGTG 8 cut(s) 1140, 1541, 1872, 2464, 2712, 2713, 2860, 2865
Cac8I GCNNGC 6 cut(s) 92, 1208, 1861, 2477, 2635, 2726
CciI TCATGA 1 cut(s) 2125
CfoI GCGC 5 cut(s) 1865, 2421, 2726, 2728, 2897
Cfr10I RCCGGY 8 cut(s) 458, 812, 1206, 1308, 1673, 1705, 2000, 2633
Cfr13I GGNCC 6 cut(s) 887, 1102, 1604, 1717, 2702, 2787
ClaI ATCGAT 2 cut(s) 1398, 1453
CseI GACGC 2 cut(s) 2534, 2725
Csp6I GTAC 4 cut(s) 497, 640, 856, 2755
CspCI CAANNNNNGTGG 2 cut(s) 1746, 1781
CviQI GTAC 4 cut(s) 497, 640, 856, 2755
DdeI CTNAG 7 cut(s) 938, 1147, 1201, 1544, 1655, 2294, 2764
DpnI GATC 9 cut(s) 550, 716, 792, 2113, 2312, 2321, 2415, 2681, 2801
DpnII GATC 9 cut(s) 548, 714, 790, 2111, 2310, 2319, 2413, 2679, 2799
EaeI YGGCCR 2 cut(s) 382, 1329
Eam1104I CTCTTC 2 cut(s) 1232, 1431
EarI CTCTTC 2 cut(s) 1232, 1431
EciI GGCGGA 1 cut(s) 1905
Ecl136II GAGCTC 1 cut(s) 289
Eco130I CCWWGG 4 cut(s) 577, 1061, 2583, 2880
Eco24I GRGCYC 3 cut(s) 291, 2673, 2752
Eco31I GGTCTC 1 cut(s) 917
Eco32I GATATC 2 cut(s) 318, 1369
Eco47I GGWCC 4 cut(s) 887, 1604, 1717, 2787
Eco53kI GAGCTC 1 cut(s) 289
Eco57I CTGAAG 4 cut(s) 137, 204, 424, 1061
Eco72I CACGTG 1 cut(s) 2812
Eco88I CYCGRG 5 cut(s) 914, 1099, 1372, 1496, 1712
Eco91I GGTNACC 1 cut(s) 764
EcoICRI GAGCTC 1 cut(s) 289
EcoO109I RGGNCCY 2 cut(s) 1717, 2702
EcoO65I GGTNACC 1 cut(s) 764
EcoRI GAATTC 2 cut(s) 675, 2544
EcoRII CCWGG 6 cut(s) 292, 607, 751, 1362, 2223, 2953
EcoRV GATATC 2 cut(s) 318, 1369
EcoT14I CCWWGG 4 cut(s) 577, 1061, 2583, 2880
EcoT38I GRGCYC 3 cut(s) 291, 2673, 2752
ErhI CCWWGG 4 cut(s) 577, 1061, 2583, 2880
Esp3I CGTCTC 2 cut(s) 83, 1124
FalI AAGNNNNNCTT 4 cut(s) 1252, 1284, 2929, 2961
FaqI GGGAC 6 cut(s) 72, 325, 374, 1427, 1590, 1730
FbaI TGATCA 2 cut(s) 2310, 2799
FblI GTMKAC 1 cut(s) 1912
Fnu4HI GCNGC 6 cut(s) 445, 1829, 1839, 1888, 2084, 2142
FokI GGATG 5 cut(s) 149, 307, 590, 1208, 1595
FriOI GRGCYC 3 cut(s) 291, 2673, 2752
Fsp4HI GCNGC 6 cut(s) 445, 1829, 1839, 1888, 2084, 2142
GlaI GCGC 5 cut(s) 1864, 2420, 2725, 2727, 2896
GluI GCNGC 6 cut(s) 445, 1829, 1839, 1888, 2084, 2142
HaeII RGCGCY 1 cut(s) 2422
HaeIII GGCC 7 cut(s) 384, 653, 693, 1104, 1206, 1331, 2703
HgaI GACGC 2 cut(s) 2534, 2725
HhaI GCGC 5 cut(s) 1865, 2421, 2726, 2728, 2897
Hin6I GCGC 5 cut(s) 1863, 2419, 2724, 2726, 2895
HinP1I GCGC 5 cut(s) 1863, 2419, 2724, 2726, 2895
HincII GTYRAC 1 cut(s) 1795
HindII GTYRAC 1 cut(s) 1795
HindIII AAGCTT 3 cut(s) 174, 1580, 2291
HphI GGTGA 6 cut(s) 49, 802, 1330, 1331, 1663, 2964
Hpy166II GTNNAC 6 cut(s) 799, 1338, 1795, 1870, 1913, 2524
Hpy8I GTNNAC 6 cut(s) 799, 1338, 1795, 1870, 1913, 2524
Hpy99I CGWCG 2 cut(s) 2741, 2744
HpyAV CCTTC 7 cut(s) 178, 1085, 1238, 2144, 2366, 2440, 2867
HpyCH4III ACNGT 6 cut(s) 466, 1144, 2056, 2345, 2457, 2468
HpyCH4IV ACGT 6 cut(s) 15, 76, 125, 399, 1117, 2811
HpyF3I CTNAG 7 cut(s) 938, 1147, 1201, 1544, 1655, 2294, 2764
HpySE526I ACGT 6 cut(s) 15, 76, 125, 399, 1117, 2811
HspAI GCGC 5 cut(s) 1863, 2419, 2724, 2726, 2895
Kpn2I TCCGGA 1 cut(s) 2614
KpnI GGTACC 1 cut(s) 859
KroI GCCGGC 2 cut(s) 1206, 2633
KroNI GCCGGC 2 cut(s) 1208, 2635
Ksp22I TGATCA 2 cut(s) 2310, 2799
Kzo9I GATC 9 cut(s) 548, 714, 790, 2111, 2310, 2319, 2413, 2679, 2799
LmnI GCTCC 4 cut(s) 294, 1769, 2080, 2617
Lsp1109I GCAGC 4 cut(s) 456, 1850, 2070, 2153
LweI GCATC 1 cut(s) 258
MaeII ACGT 6 cut(s) 15, 76, 125, 399, 1117, 2811
MalI GATC 9 cut(s) 550, 716, 792, 2113, 2312, 2321, 2415, 2681, 2801
MboI GATC 9 cut(s) 548, 714, 790, 2111, 2310, 2319, 2413, 2679, 2799
MfeI CAATTG 2 cut(s) 238, 1619
MflI RGATCY 1 cut(s) 714
MhlI GDGCHC 4 cut(s) 291, 2358, 2673, 2752
MlyI GAGTC 5 cut(s) 1193, 2004, 2563, 2573, 2786
MmeI TCCRAC 5 cut(s) 534, 649, 1006, 1542, 2416
MroI TCCGGA 1 cut(s) 2614
MroNI GCCGGC 2 cut(s) 1206, 2633
MroXI GAANNNNTTC 1 cut(s) 2568
MseI TTAA 9 cut(s) 24, 45, 455, 644, 1215, 1932, 2018, 2049, 2960
MslI CAYNNNNRTG 4 cut(s) 95, 2165, 2304, 2778
MspR9I CCNGG 6 cut(s) 294, 609, 753, 1364, 2225, 2955
MunI CAATTG 2 cut(s) 238, 1619
Mva1269I GAATGC 1 cut(s) 2220
MvaI CCWGG 6 cut(s) 294, 609, 753, 1364, 2225, 2955
MvnI CGCG 2 cut(s) 2726, 2897
NaeI GCCGGC 2 cut(s) 1208, 2635
NcoI CCATGG 1 cut(s) 2880
NdeII GATC 9 cut(s) 548, 714, 790, 2111, 2310, 2319, 2413, 2679, 2799
NgoMIV GCCGGC 2 cut(s) 1206, 2633
NlaIV GGNNCC 6 cut(s) 613, 857, 1606, 1718, 2300, 2704
NmeAIII GCCGAG 3 cut(s) 1313, 1357, 2505
NmuCI GTSAC 6 cut(s) 941, 1895, 1960, 2268, 2708, 2873
NspI RCATGY 4 cut(s) 94, 258, 1420, 1825
PaeI GCATGC 1 cut(s) 94
PaeR7I CTCGAG 2 cut(s) 914, 1496
PagI TCATGA 1 cut(s) 2125
PauI GCGCGC 1 cut(s) 2724
PcsI WCGNNNNNNNCGW 4 cut(s) 354, 921, 1647, 2679
PctI GAATGC 1 cut(s) 2220
PdiI GCCGGC 2 cut(s) 1208, 2635
PdmI GAANNNNTTC 1 cut(s) 2568
PfeI GAWTC 9 cut(s) 781, 1395, 1681, 1753, 2096, 2198, 2237, 2589, 2921
PflMI CCANNNNNTGG 1 cut(s) 994
PfoI TCCNGGA 2 cut(s) 1362, 2953
PkrI GCNGC 6 cut(s) 446, 1830, 1840, 1889, 2085, 2143
PleI GAGTC 5 cut(s) 1193, 2003, 2562, 2573, 2786
PmaCI CACGTG 1 cut(s) 2812
PmlI CACGTG 1 cut(s) 2812
PpsI GAGTC 5 cut(s) 1193, 2003, 2562, 2573, 2786
Ppu21I YACGTR 3 cut(s) 16, 126, 2812
PpuMI RGGWCCY 1 cut(s) 1717
PshBI ATTAAT 1 cut(s) 24
PsiI TTATAA 1 cut(s) 407
Psp124BI GAGCTC 1 cut(s) 291
Psp5II RGGWCCY 1 cut(s) 1717
Psp6I CCWGG 6 cut(s) 292, 607, 751, 1362, 2223, 2953
PspCI CACGTG 1 cut(s) 2812
PspEI GGTNACC 1 cut(s) 764
PspGI CCWGG 6 cut(s) 292, 607, 751, 1362, 2223, 2953
PspN4I GGNNCC 6 cut(s) 613, 857, 1606, 1718, 2300, 2704
PspPI GGNCC 6 cut(s) 887, 1102, 1604, 1717, 2702, 2787
PspPPI RGGWCCY 1 cut(s) 1717
PsrI GAACNNNNNNTAC 2 cut(s) 1770, 1802
PstI CTGCAG 2 cut(s) 1302, 2088
PsuI RGATCY 1 cut(s) 714
PteI GCGCGC 1 cut(s) 2724
RsaI GTAC 4 cut(s) 498, 641, 857, 2756
RsaNI GTAC 4 cut(s) 497, 640, 856, 2755
RseI CAYNNNNRTG 4 cut(s) 95, 2165, 2304, 2778
SacI GAGCTC 1 cut(s) 291
SaqAI TTAA 9 cut(s) 24, 45, 455, 644, 1215, 1932, 2018, 2049, 2960
SatI GCNGC 6 cut(s) 445, 1829, 1839, 1888, 2084, 2142
Sau3AI GATC 9 cut(s) 548, 714, 790, 2111, 2310, 2319, 2413, 2679, 2799
Sau96I GGNCC 6 cut(s) 887, 1102, 1604, 1717, 2702, 2787
ScaI AGTACT 1 cut(s) 641
SchI GAGTC 5 cut(s) 1193, 2004, 2563, 2573, 2786
ScrFI CCNGG 6 cut(s) 294, 609, 753, 1364, 2225, 2955
SduI GDGCHC 4 cut(s) 291, 2358, 2673, 2752
SfaNI GCATC 1 cut(s) 258
SfcI CTRYAG 3 cut(s) 1298, 1773, 2084
Sfr274I CTCGAG 2 cut(s) 914, 1496
SgrAI CRCCGGYG 1 cut(s) 812
SinI GGWCC 4 cut(s) 887, 1604, 1717, 2787
SlaI CTCGAG 2 cut(s) 914, 1496
SmiMI CAYNNNNRTG 4 cut(s) 95, 2165, 2304, 2778
SmlI CTYRAG 3 cut(s) 914, 1282, 1496
SmoI CTYRAG 3 cut(s) 914, 1282, 1496
SphI GCATGC 1 cut(s) 94
SsiI CCGC 5 cut(s) 1666, 1829, 1887, 1890, 2432
SspI AATATT 1 cut(s) 1326
SstI GAGCTC 1 cut(s) 291
StyD4I CCNGG 6 cut(s) 292, 607, 751, 1362, 2223, 2953
StyI CCWWGG 4 cut(s) 577, 1061, 2583, 2880
TaaI ACNGT 6 cut(s) 466, 1144, 2056, 2345, 2457, 2468
TaiI ACGT 6 cut(s) 18, 79, 128, 402, 1120, 2814
TaqII GACCGA 1 cut(s) 2804
TatI WGTACW 1 cut(s) 639
TauI GCSGC 2 cut(s) 1831, 1890
TfiI GAWTC 9 cut(s) 781, 1395, 1681, 1753, 2096, 2198, 2237, 2589, 2921
Tru1I TTAA 9 cut(s) 24, 45, 455, 644, 1215, 1932, 2018, 2049, 2960
Tru9I TTAA 9 cut(s) 24, 45, 455, 644, 1215, 1932, 2018, 2049, 2960
TscAI CASTG 8 cut(s) 1147, 1548, 1872, 2471, 2713, 2719, 2867, 2872
TseFI GTSAC 6 cut(s) 941, 1895, 1960, 2268, 2708, 2873
TseI GCWGC 4 cut(s) 444, 1838, 2083, 2141
Tsp45I GTSAC 6 cut(s) 941, 1895, 1960, 2268, 2708, 2873
TspDTI ATGAA 8 cut(s) 18, 713, 1608, 1617, 2114, 2142, 2861, 2916
TspGWI ACGGA 3 cut(s) 1774, 1908, 2591
TspRI CASTG 8 cut(s) 1147, 1548, 1872, 2471, 2713, 2719, 2867, 2872
Van91I CCANNNNNTGG 1 cut(s) 994
VpaK11BI GGWCC 4 cut(s) 887, 1604, 1717, 2787
VspI ATTAAT 1 cut(s) 24
XapI RAATTY 9 cut(s) 566, 586, 675, 1035, 1477, 1762, 2130, 2544, 2717
XbaI TCTAGA 2 cut(s) 931, 2108
XceI RCATGY 4 cut(s) 94, 258, 1420, 1825
XhoI CTCGAG 2 cut(s) 914, 1496
XmiI GTMKAC 1 cut(s) 1912
XmnI GAANNNNTTC 1 cut(s) 2568
ZrmI AGTACT 1 cut(s) 641
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.