MD15G1092100.v1.1
NAC Family

Probably inactive leucine-rich repeat receptor-like protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0008421
Physical Location & Seq
Forward (+)
6392342 .. 6395580
3239 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1092100.v1.1.491

Sequence Viewer

Length: 2943 bp
ATGATGATCAAGATGGAAAAGAGACAACCTCATCAAACACGTCTCATATGGTGCATGCTCGTGTTCTTATTCTTCTTCTCAATTCATACTTGTGCTTTGGATGGTAGTGAGGAACGTCAGCTTCTCTTATCCTTCAAAGCTTCTGTCAACGACCCTTCACACTACCTCTCAAACTGGAACACTTCAGCCAACACTTTATGCAATTGGCATGGCATCACATGCAACGACAATAACAACATCAAGGCCATCGAGCTCACCGGAAGAAACATCTCCGGCAAGTTATCATCCTCCATCTTCCACTTGTCTCACATTGAAACCATCGACCTCTCCAACAATCAGCTATCCGGCCAACTCCCCAATGACATGTTCATCAGTGGGTCAAATTCGCTTCGCCGTCTCAATTTCAGCAACAACATCTTAACCAGCACACTTCCACAAGGCTCACTCACAACCCTTGAGGTACTAGACTTGTCCAACAACATGATTTCAGGCATAATCCCAAACAACGTCGGATTGCTTTCTACGTTGAAGTTTCTTGACCTTGGAGGAAATATGTTGGTGGGAACCATCCCTAGCTCCATATCAAACATGTCTAGCTTGGAGTACTTGACTTTGGCATCAAACCAACTTGTTGGAAAAATTCCAGCACAATTAGGCCAAATGAAGAACTTGAAGTGGATTTACTTGGGCTACAACAATCTCTCCGGCAATATACCGGAACAAATTGGTAACCTTTTTCTGTTAAATCATCTCAATCTTGTTTACAACAATCTCACCGGCGAAATCCCATTCACAATCAGCAACCTTACTAATCTTCGGTACCTTTTCCTCTACGGAAACAAACTCACAGGTCCAGTTCCACAATCCTTATTCAGTCTCGAGAAACTCGTTTCTCTCGACCTTAGTGACAATTTTCTTTCAGGAGAGATTCCCGAATGTGTTTTCAACCTCCAAAACGTGGAAATCCTCCATCTTTTTTCAAACAACTTCACGGGAAAAATTCCCAAAGCTCTGGCTTCTCTGCCAAGGCTTCAAGTCCTTCAGTTATGGTCAAACAAGTTCTCGGGCCAAATCCCAAAACGTCTCGGAAATCAAAACAGCCTCACTGTCTTAGACCTTTCCTCAAACAACCTTACTGGGAAAATACCCGATACGCTATGCGACTCGGGCCGGCTTTTTAAGCTCATCCTCTTCTCGAATTCACTCGAAGGCGCAATCCCGCGAAGCTTGTCTTCTTGCAAGAGCTTGAGCAGAGTCAGACTCCAGAACAACAGGCTTTCAGGTGAATTATTGGCAGAGTTCACCAAACTCCCACTTGTCTATTTCCTGGATATCTCGGGCAACAATCTCTCTGGAAGAATCGACGACAGAAAGTGGGACATGCCATCTCTTCAAATGCTGAATATGGCGAGAAATCGATTTTTCGGGAAGTTACCGGAAACTTTCGGGGGCCAAAAGCTGGAGAACTTGGACTTGTCCGAAAATTGGTTCTCAGGAAGTATCTTGCCGAGTTTCGGGAACTTTCCGGAGCTAATGCAATTGAAGCTTTCTCACAATGAGCTCTCTGGTCCCATCCCTCAACAATTGTCTTCATGCAAAAAACTCGTTAGCCTCGACCTCAGCCACAACCGCCTCACCGGCACGATTCCCACTAGCCTCTCTGATATGCCGGTTCTGGGGGACCTAGATTTGTCCGAAAACCAAATATCGGGCGAGGTTCCGCTGAATTTGGGCGCTAAAGTATCGCTTGTTCAAGTCAACATTTCCCACAACAAACTCCACGGCATATTGCCGCCCACAGCAGTGTTTCTTGCCATAGATGCCAGCGCAGTGGACGGCAACAACCTCTGTGGTGGCGGTGACACTATGAGTGCTCTACCGCCGTGTAAGAGCGTTAAGAGAAACCCTACTTGGTGGTTTATTGTCACGTGTTTTCTTGTTGCATTATTGGCTTTTGGAGTTGCCAGTTATTTGTTTGTGTTATTGAGGAGGAGAAAGGAATTGGAGGTTAAATCGGTGGAGATCCAAGAAAGGATTTGGGAGCTGCAGTTTTTCGAATCAAAGGTTTCAAGATCGGTTACGATTCATGACATTTTATCGGCAGCCAAAGAAGATAATATCATTGCAAAGGGTAAGAATGGGATTTCATATAAAGGAGAATCGGTTTCCAACGGAATGCAATTCGTGGTAAAAGAGGATTCTGTGAAATCACTTCCACCAAGCTTTTGGTCACAGATGGTTGAACTTGGGAAGCTTAGGCACCCCAATGTGATCAAACTGATCGGAATATGTCATTCCGAAAACGATGCGTATGTGCTTTATGAGTATTGTGAAGGGAAAGTGTTGACTCAAGTTATGAGAGATTTGAGTTGGGATCAGCGCCGGAAAATTGCCGTTGGGATTGCGAAAGCACTACGTTTCTTGCATTGTTGTTGCTCGCCGAGCCTTGTAGCTGGTTGTATGTCACCAGAGAAAGTGATTGTGGACGCAAAAGATGAGCCTCATATTCGATTGAGTCTCTCCGCACAAGTTCGGACGGACTCCAGGGGATTCATTGCTTCTGCATACATTGCTCCGGATGCTAAAGAAAGCAAAGTCATTACAGAGAAGAGTGACATATATGGGTTTGGGCTCGTCTTGATCGAATTGCTGACCGGAAAAGGCCCCACCGACACTGAATTTGGCGCGCACCAGAGCGTCGTCGAATGGGCACGGTACTGCTACTCTGACTGCCATCTTGATGTCTGGACAGACTTGATGATCAGAGAACACGTGTCGAGCAATCAGAACGAGATTGTGGAAACCTTGAACCTAGCTCTTCACTGCACTGCTGGTGACCCCACTGCTAGACCATGCGCGAATGAACTGTATAAAACCCTAGACTCTATTCTGAGGACAAGTCGTTCTTGTGTTCCTGACCTAAAAGTTTCTTCACCTTTTTGA

Protein Analysis

981

Amino Acids

108.74

Weight (kDa)

7.26

Isoelectric Point (pI)

38.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 37 - 75 1.4e-12 Leucine rich repeat N-terminal domain
LRR_14 PF23598 130 - 227 5.9e-08 Leucine-rich repeat region
LRR_8 PF13855 199 - 258 2.7e-06 Leucine rich repeat
LRR_14 PF23598 218 - 297 3.5e-06 Leucine-rich repeat region
LRR_8 PF13855 248 - 304 3.2e-07 Leucine rich repeat
LRR_14 PF23598 266 - 389 7.4e-11 Leucine-rich repeat region
LRR_14 PF23598 337 - 574 9.5e-06 Leucine-rich repeat region
LRR_8 PF13855 342 - 402 5.2e-07 Leucine rich repeat
LRR_8 PF13855 486 - 545 1.2e-06 Leucine rich repeat
LRR_8 PF13855 509 - 569 7.6e-09 Leucine rich repeat
LRR_8 PF13855 534 - 593 6.7e-06 Leucine rich repeat
Pkinase PF00069 706 - 958 3.2e-19 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 708 - 960 1.1e-17 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 819
AccB1I GGYRCC 2 cut(s) 819, 2259
AccB7I CCANNNNNTGG 2 cut(s) 958, 1459
AccII CGCG 3 cut(s) 1222, 2687, 2858
AccIII TCCGGA 2 cut(s) 1525, 2575
AciI CCGC 7 cut(s) 1220, 1630, 1721, 1793, 1857, 1880, 2523
AclWI GGATC 2 cut(s) 2017, 2382
AcoI YGGCCR 1 cut(s) 346
AcsI RAATTY 6 cut(s) 382, 639, 999, 1198, 1726, 2678
AcuI CTGAAG 2 cut(s) 168, 1025
AcvI CACGTG 2 cut(s) 1929, 2773
AfaI GTAC 4 cut(s) 462, 605, 821, 2717
AfiI CCNNNNNNNGG 6 cut(s) 958, 1459, 1485, 1514, 1676, 1708
AflIII ACRYGT 6 cut(s) 38, 363, 588, 1928, 2770, 2772
AjiI CACGTC 1 cut(s) 41
AjnI CCWGG 2 cut(s) 1326, 2543
AjuI GAANNNNNNNTTGG 4 cut(s) 350, 382, 1894, 1926
AleI CACNNNNGTG 1 cut(s) 1802
AloI GAACNNNNNNTCC 2 cut(s) 2887, 2919
Alw21I GWGCWC 3 cut(s) 255, 1563, 1876
Alw26I GTCTC 7 cut(s) 16, 47, 309, 401, 881, 1088, 2522
AlwI GGATC 2 cut(s) 2017, 2382
Ama87I CYCGRG 4 cut(s) 878, 1063, 1165, 1336
Aor13HI TCCGGA 2 cut(s) 1525, 2575
AoxI GGCC 7 cut(s) 243, 346, 655, 1066, 1168, 1450, 2662
ApeKI GCWGC 2 cut(s) 2044, 2102
ApoI RAATTY 6 cut(s) 382, 639, 999, 1198, 1726, 2678
ArsI GACNNNNNNTTYG 4 cut(s) 129, 161, 2663, 2695
Asp718I GGTACC 1 cut(s) 819
AspLEI GCGC 7 cut(s) 1214, 1736, 1829, 2382, 2687, 2689, 2858
AspS9I GGNCC 7 cut(s) 851, 1066, 1168, 1450, 1568, 1681, 2663
AsuHPI GGTGA 9 cut(s) 247, 766, 1294, 1295, 1627, 1871, 2457, 2846, 2925
AsuII TTCGAA 1 cut(s) 2055
AvaI CYCGRG 4 cut(s) 878, 1063, 1165, 1336
AvaII GGWCC 3 cut(s) 851, 1568, 1681
BaeGI GKGCMC 1 cut(s) 2713
BanI GGYRCC 2 cut(s) 819, 2259
BanII GRGCYC 3 cut(s) 255, 1563, 2634
BarI GAAGNNNNNNTAC 2 cut(s) 665, 697
BauI CACGAG 1 cut(s) 59
BbrPI CACGTG 2 cut(s) 1929, 2773
BbsI GAAGAC 2 cut(s) 1224, 1581
Bbv12I GWGCWC 3 cut(s) 255, 1563, 1876
BbvCI CCTCAGC 1 cut(s) 1619
BbvI GCAGC 2 cut(s) 2031, 2114
BceAI ACGGC 5 cut(s) 378, 1798, 1852, 1867, 2378
BcgI CGANNNNNNTGC 4 cut(s) 1585, 1619, 2288, 2322
BciT130I CCWGG 2 cut(s) 1328, 2545
BclI TGATCA 3 cut(s) 6, 2271, 2760
BcoDI GTCTC 7 cut(s) 16, 47, 309, 401, 881, 1088, 2522
BfaI CTAG 8 cut(s) 464, 573, 594, 1653, 1685, 2813, 2847, 2879
BfmI CTRYAG 1 cut(s) 2045
BfoI RGCGCY 2 cut(s) 1737, 2383
BglI GCCNNNNNGGC 1 cut(s) 1638
BisI GCNGC 3 cut(s) 1793, 2045, 2103
BlsI GCNGC 3 cut(s) 1794, 2046, 2104
BmcAI AGTACT 1 cut(s) 605
Bme1390I CCNGG 2 cut(s) 1328, 2545
Bme18I GGWCC 3 cut(s) 851, 1568, 1681
BmeT110I CYCGRG 4 cut(s) 878, 1063, 1165, 1336
BmgBI CACGTC 1 cut(s) 41
BmgT120I GGNCC 7 cut(s) 851, 1066, 1168, 1450, 1568, 1681, 2663
BmiI GGNNCC 8 cut(s) 565, 821, 1451, 1570, 1682, 1719, 2261, 2665
BmrFI CCNGG 2 cut(s) 1328, 2545
BmrI ACTGGG 1 cut(s) 1146
BmsI GCATC 5 cut(s) 222, 626, 1810, 2296, 2569
BmuI ACTGGG 1 cut(s) 1146
BpiI GAAGAC 2 cut(s) 1224, 1581
BplI GAGNNNNNCTC 4 cut(s) 13, 45, 1245, 1277
BpmI CTGGAG 3 cut(s) 1247, 1481, 2527
Bpu10I CCTNAGC 2 cut(s) 1619, 2255
Bpu14I TTCGAA 1 cut(s) 2055
BpuEI CTTGAG 3 cut(s) 476, 1267, 2334
Bsa29I ATCGAT 1 cut(s) 1417
BsaAI YACGTR 2 cut(s) 1929, 2773
BsaJI CCNNGG 4 cut(s) 541, 1025, 1780, 2544
BsaWI WCCGGW 6 cut(s) 257, 715, 1435, 1525, 2575, 2654
BsaXI ACNNNNNCTCC 6 cut(s) 686, 716, 2033, 2063, 2148, 2178
Bsc4I CCNNNNNNNGG 6 cut(s) 958, 1459, 1485, 1514, 1676, 1708
Bse118I RCCGGY 4 cut(s) 776, 1170, 1637, 1669
Bse1I ACTGG 4 cut(s) 179, 854, 1141, 1965
Bse3DI GCAATG 3 cut(s) 2121, 2553, 2568
BseAI TCCGGA 2 cut(s) 1525, 2575
BseBI CCWGG 2 cut(s) 1328, 2545
BseCI ATCGAT 1 cut(s) 1417
BseDI CCNNGG 4 cut(s) 541, 1025, 1780, 2544
BseGI GGATG 6 cut(s) 106, 284, 567, 1185, 1572, 2584
BseLI CCNNNNNNNGG 6 cut(s) 958, 1459, 1485, 1514, 1676, 1708
BseMI GCAATG 3 cut(s) 2121, 2553, 2568
BseMII CTCAG 3 cut(s) 1506, 1633, 2882
BseNI ACTGG 4 cut(s) 179, 854, 1141, 1965
BsePI GCGCGC 1 cut(s) 2685
BseRI GAGGAG 2 cut(s) 2002, 2005
BseSI GKGCMC 1 cut(s) 2713
BseXI GCAGC 2 cut(s) 2031, 2114
BsgI GTGCAG 1 cut(s) 2809
Bsh1236I CGCG 3 cut(s) 1222, 2687, 2858
BshFI GGCC 7 cut(s) 245, 348, 657, 1068, 1170, 1452, 2664
BshNI GGYRCC 2 cut(s) 819, 2259
BshVI ATCGAT 1 cut(s) 1417
BsiHKAI GWGCWC 3 cut(s) 255, 1563, 1876
BsiHKCI CYCGRG 4 cut(s) 878, 1063, 1165, 1336
BslFI GGGAC 3 cut(s) 1391, 1554, 1694
BslI CCNNNNNNNGG 6 cut(s) 958, 1459, 1485, 1514, 1676, 1708
BsmAI GTCTC 7 cut(s) 16, 47, 309, 401, 881, 1088, 2522
BsmBI CGTCTC 3 cut(s) 47, 401, 1088
BsmFI GGGAC 3 cut(s) 1391, 1554, 1694
BsmI GAATGC 1 cut(s) 2181
BsnI GGCC 7 cut(s) 245, 348, 657, 1068, 1170, 1452, 2664
BsoBI CYCGRG 4 cut(s) 878, 1063, 1165, 1336
Bsp119I TTCGAA 1 cut(s) 2055
Bsp1286I GDGCHC 5 cut(s) 255, 1563, 1876, 2634, 2713
Bsp13I TCCGGA 2 cut(s) 1525, 2575
Bsp143I GATC 8 cut(s) 6, 2022, 2072, 2271, 2280, 2374, 2640, 2760
BspACI CCGC 7 cut(s) 1220, 1630, 1721, 1793, 1857, 1880, 2523
BspANI GGCC 7 cut(s) 245, 348, 657, 1068, 1170, 1452, 2664
BspCNI CTCAG 3 cut(s) 1505, 1632, 2883
BspDI ATCGAT 1 cut(s) 1417
BspEI TCCGGA 2 cut(s) 1525, 2575
BspFNI CGCG 3 cut(s) 1222, 2687, 2858
BspHI TCATGA 1 cut(s) 2086
BspLI GGNNCC 8 cut(s) 565, 821, 1451, 1570, 1682, 1719, 2261, 2665
BspMAI CTGCAG 1 cut(s) 2049
BspPI GGATC 2 cut(s) 2017, 2382
BspQI GCTCTTC 1 cut(s) 2823
BspT104I TTCGAA 1 cut(s) 2055
BspT107I GGYRCC 2 cut(s) 819, 2259
BsrDI GCAATG 3 cut(s) 2121, 2553, 2568
BsrFI RCCGGY 4 cut(s) 776, 1170, 1637, 1669
BsrI ACTGG 4 cut(s) 179, 854, 1141, 1965
BssAI RCCGGY 4 cut(s) 776, 1170, 1637, 1669
BssECI CCNNGG 4 cut(s) 541, 1025, 1780, 2544
BssHII GCGCGC 1 cut(s) 2685
BssMI GATC 8 cut(s) 6, 2022, 2072, 2271, 2280, 2374, 2640, 2760
BssSI CACGAG 1 cut(s) 59
BssT1I CCWWGG 2 cut(s) 541, 1025
Bst2BI CACGAG 1 cut(s) 59
Bst2UI CCWGG 2 cut(s) 1328, 2545
Bst4CI ACNGT 3 cut(s) 1108, 2715, 2868
Bst6I CTCTTC 4 cut(s) 1196, 1395, 2603, 2823
BstAPI GCANNNNNTGC 2 cut(s) 219, 2570
BstBAI YACGTR 2 cut(s) 1929, 2773
BstBI TTCGAA 1 cut(s) 2055
BstC8I GCNNGC 5 cut(s) 56, 1172, 1825, 2438, 2687
BstDEI CTNAG 6 cut(s) 902, 1111, 1492, 1619, 2255, 2891
BstDSI CCRYGG 1 cut(s) 1780
BstEII GGTNACC 2 cut(s) 728, 2834
BstF5I GGATG 6 cut(s) 106, 284, 567, 1185, 1572, 2584
BstFNI CGCG 3 cut(s) 1222, 2687, 2858
BstH2I RGCGCY 2 cut(s) 1737, 2383
BstHHI GCGC 7 cut(s) 1214, 1736, 1829, 2382, 2687, 2689, 2858
BstKTI GATC 8 cut(s) 9, 2025, 2075, 2274, 2283, 2377, 2643, 2763
BstMAI GTCTC 7 cut(s) 16, 47, 309, 401, 881, 1088, 2522
BstMBI GATC 8 cut(s) 6, 2022, 2072, 2271, 2280, 2374, 2640, 2760
BstNI CCWGG 2 cut(s) 1328, 2545
BstNSI RCATGY 5 cut(s) 58, 222, 367, 592, 1384
BstPI GGTNACC 2 cut(s) 728, 2834
BstSCI CCNGG 2 cut(s) 1326, 2543
BstSFI CTRYAG 1 cut(s) 2045
BstSLI GKGCMC 1 cut(s) 2713
BstUI CGCG 3 cut(s) 1222, 2687, 2858
BstV1I GCAGC 2 cut(s) 2031, 2114
BstV2I GAAGAC 2 cut(s) 1224, 1581
BstX2I RGATCY 1 cut(s) 2022
BstXI CCANNNNNNTGG 4 cut(s) 632, 1012, 1831, 2226
BstYI RGATCY 1 cut(s) 2022
Bsu15I ATCGAT 1 cut(s) 1417
BsuRI GGCC 7 cut(s) 245, 348, 657, 1068, 1170, 1452, 2664
BsuTUI ATCGAT 1 cut(s) 1417
BtgI CCRYGG 1 cut(s) 1780
BtrI CACGTC 1 cut(s) 41
BtsCI GGATG 6 cut(s) 106, 284, 567, 1185, 1572, 2584
BtsI GCAGTG 5 cut(s) 1809, 1836, 2821, 2826, 2841
BtsIMutI CAGTG 8 cut(s) 379, 1104, 1809, 1836, 2673, 2821, 2826, 2841
Cac8I GCNNGC 5 cut(s) 56, 1172, 1825, 2438, 2687
CciI TCATGA 1 cut(s) 2086
CfoI GCGC 7 cut(s) 1214, 1736, 1829, 2382, 2687, 2689, 2858
Cfr10I RCCGGY 4 cut(s) 776, 1170, 1637, 1669
Cfr13I GGNCC 7 cut(s) 851, 1066, 1168, 1450, 1568, 1681, 2663
ClaI ATCGAT 1 cut(s) 1417
CseI GACGC 2 cut(s) 2495, 2686
Csp6I GTAC 4 cut(s) 461, 604, 820, 2716
CspCI CAANNNNNGTGG 2 cut(s) 1831, 1866
CviQI GTAC 4 cut(s) 461, 604, 820, 2716
DdeI CTNAG 6 cut(s) 902, 1111, 1492, 1619, 2255, 2891
DpnI GATC 8 cut(s) 8, 2024, 2074, 2273, 2282, 2376, 2642, 2762
DpnII GATC 8 cut(s) 6, 2022, 2072, 2271, 2280, 2374, 2640, 2760
EaeI YGGCCR 1 cut(s) 346
Eam1104I CTCTTC 4 cut(s) 1196, 1395, 2603, 2823
EarI CTCTTC 4 cut(s) 1196, 1395, 2603, 2823
Ecl136II GAGCTC 2 cut(s) 253, 1561
Eco130I CCWWGG 2 cut(s) 541, 1025
Eco24I GRGCYC 3 cut(s) 255, 1563, 2634
Eco32I GATATC 1 cut(s) 1333
Eco47I GGWCC 3 cut(s) 851, 1568, 1681
Eco53kI GAGCTC 2 cut(s) 253, 1561
Eco57I CTGAAG 2 cut(s) 168, 1025
Eco72I CACGTG 2 cut(s) 1929, 2773
Eco88I CYCGRG 4 cut(s) 878, 1063, 1165, 1336
Eco91I GGTNACC 2 cut(s) 728, 2834
EcoICRI GAGCTC 2 cut(s) 253, 1561
EcoO109I RGGNCCY 2 cut(s) 1681, 2663
EcoO65I GGTNACC 2 cut(s) 728, 2834
EcoRI GAATTC 1 cut(s) 1198
EcoRII CCWGG 2 cut(s) 1326, 2543
EcoRV GATATC 1 cut(s) 1333
EcoT14I CCWWGG 2 cut(s) 541, 1025
EcoT38I GRGCYC 3 cut(s) 255, 1563, 2634
ErhI CCWWGG 2 cut(s) 541, 1025
Esp3I CGTCTC 3 cut(s) 47, 401, 1088
FalI AAGNNNNNCTT 6 cut(s) 1216, 1248, 1731, 1763, 2890, 2922
FaqI GGGAC 3 cut(s) 1391, 1554, 1694
FauI CCCGC 1 cut(s) 1227
FauNDI CATATG 1 cut(s) 47
FbaI TGATCA 3 cut(s) 6, 2271, 2760
Fnu4HI GCNGC 3 cut(s) 1793, 2045, 2103
FokI GGATG 6 cut(s) 113, 271, 554, 1172, 1559, 2591
FriOI GRGCYC 3 cut(s) 255, 1563, 2634
Fsp4HI GCNGC 3 cut(s) 1793, 2045, 2103
FspBI CTAG 8 cut(s) 464, 573, 594, 1653, 1685, 2813, 2847, 2879
GlaI GCGC 7 cut(s) 1213, 1735, 1828, 2381, 2686, 2688, 2857
GluI GCNGC 3 cut(s) 1793, 2045, 2103
GsuI CTGGAG 3 cut(s) 1247, 1481, 2527
HaeII RGCGCY 2 cut(s) 1737, 2383
HaeIII GGCC 7 cut(s) 245, 348, 657, 1068, 1170, 1452, 2664
HgaI GACGC 2 cut(s) 2495, 2686
HhaI GCGC 7 cut(s) 1214, 1736, 1829, 2382, 2687, 2689, 2858
Hin6I GCGC 7 cut(s) 1212, 1734, 1827, 2380, 2685, 2687, 2856
HinP1I GCGC 7 cut(s) 1212, 1734, 1827, 2380, 2685, 2687, 2856
HincII GTYRAC 3 cut(s) 148, 1759, 2346
HindII GTYRAC 3 cut(s) 148, 1759, 2346
HindIII AAGCTT 5 cut(s) 138, 1225, 1544, 2221, 2252
HphI GGTGA 9 cut(s) 247, 766, 1294, 1295, 1627, 1871, 2457, 2846, 2925
Hpy166II GTNNAC 7 cut(s) 148, 763, 1302, 1759, 1834, 2346, 2485
Hpy8I GTNNAC 7 cut(s) 148, 763, 1302, 1759, 1834, 2346, 2485
Hpy99I CGWCG 4 cut(s) 512, 1367, 2702, 2705
HpyAV CCTTC 5 cut(s) 142, 165, 1049, 1202, 2327
HpyCH4III ACNGT 3 cut(s) 1108, 2715, 2868
HpyCH4IV ACGT 9 cut(s) 40, 115, 507, 524, 957, 1081, 1928, 2416, 2772
HpyF3I CTNAG 6 cut(s) 902, 1111, 1492, 1619, 2255, 2891
HpySE526I ACGT 9 cut(s) 40, 115, 507, 524, 957, 1081, 1928, 2416, 2772
HspAI GCGC 7 cut(s) 1212, 1734, 1827, 2380, 2685, 2687, 2856
Kpn2I TCCGGA 2 cut(s) 1525, 2575
KpnI GGTACC 1 cut(s) 823
KroI GCCGGC 1 cut(s) 1170
KroNI GCCGGC 1 cut(s) 1172
Ksp22I TGATCA 3 cut(s) 6, 2271, 2760
Kzo9I GATC 8 cut(s) 6, 2022, 2072, 2271, 2280, 2374, 2640, 2760
LguI GCTCTTC 1 cut(s) 2823
LmnI GCTCC 4 cut(s) 581, 1528, 2041, 2578
Lsp1109I GCAGC 2 cut(s) 2031, 2114
LweI GCATC 5 cut(s) 222, 626, 1810, 2296, 2569
MaeI CTAG 8 cut(s) 464, 573, 594, 1653, 1685, 2813, 2847, 2879
MaeII ACGT 9 cut(s) 40, 115, 507, 524, 957, 1081, 1928, 2416, 2772
MalI GATC 8 cut(s) 8, 2024, 2074, 2273, 2282, 2376, 2642, 2762
MboI GATC 8 cut(s) 6, 2022, 2072, 2271, 2280, 2374, 2640, 2760
MfeI CAATTG 3 cut(s) 202, 1538, 1583
MflI RGATCY 1 cut(s) 2022
MhlI GDGCHC 5 cut(s) 255, 1563, 1876, 2634, 2713
MlyI GAGTC 7 cut(s) 1157, 1254, 1263, 2341, 2524, 2534, 2876
MmeI TCCRAC 5 cut(s) 354, 490, 498, 613, 2193
MroI TCCGGA 2 cut(s) 1525, 2575
MroNI GCCGGC 1 cut(s) 1170
MseI TTAA 5 cut(s) 419, 743, 1179, 1896, 2010
MslI CAYNNNNRTG 5 cut(s) 59, 90, 1802, 2265, 2739
MspA1I CMGCKG 1 cut(s) 1723
MspR9I CCNGG 2 cut(s) 1328, 2545
MunI CAATTG 3 cut(s) 202, 1538, 1583
Mva1269I GAATGC 1 cut(s) 2181
MvaI CCWGG 2 cut(s) 1328, 2545
MvnI CGCG 3 cut(s) 1222, 2687, 2858
NaeI GCCGGC 1 cut(s) 1172
NdeI CATATG 1 cut(s) 47
NdeII GATC 8 cut(s) 6, 2022, 2072, 2271, 2280, 2374, 2640, 2760
NgoMIV GCCGGC 1 cut(s) 1170
NlaIV GGNNCC 8 cut(s) 565, 821, 1451, 1570, 1682, 1719, 2261, 2665
NmeAIII GCCGAG 2 cut(s) 1533, 2466
NmuCI GTSAC 7 cut(s) 905, 1859, 1924, 2229, 2463, 2612, 2834
NspI RCATGY 5 cut(s) 58, 222, 367, 592, 1384
NspV TTCGAA 1 cut(s) 2055
OliI CACNNNNGTG 1 cut(s) 1802
PaeI GCATGC 1 cut(s) 58
PaeR7I CTCGAG 1 cut(s) 878
PagI TCATGA 1 cut(s) 2086
PauI GCGCGC 1 cut(s) 2685
PciI ACATGT 2 cut(s) 363, 588
PciSI GCTCTTC 1 cut(s) 2823
PcsI WCGNNNNNNNCGW 4 cut(s) 885, 894, 1611, 2640
PctI GAATGC 1 cut(s) 2181
PdiI GCCGGC 1 cut(s) 1172
PfeI GAWTC 8 cut(s) 928, 1359, 1645, 2057, 2083, 2159, 2198, 2550
PflMI CCANNNNNTGG 2 cut(s) 958, 1459
PfoI TCCNGGA 1 cut(s) 1326
PkrI GCNGC 3 cut(s) 1794, 2046, 2104
PleI GAGTC 7 cut(s) 1157, 1254, 1262, 2341, 2523, 2534, 2876
PmaCI CACGTG 2 cut(s) 1929, 2773
PmlI CACGTG 2 cut(s) 1929, 2773
PpsI GAGTC 7 cut(s) 1157, 1254, 1262, 2341, 2523, 2534, 2876
Ppu21I YACGTR 2 cut(s) 1929, 2773
PpuMI RGGWCCY 1 cut(s) 1681
PscI ACATGT 2 cut(s) 363, 588
Psp124BI GAGCTC 2 cut(s) 255, 1563
Psp5II RGGWCCY 1 cut(s) 1681
Psp6I CCWGG 2 cut(s) 1326, 2543
PspCI CACGTG 2 cut(s) 1929, 2773
PspEI GGTNACC 2 cut(s) 728, 2834
PspGI CCWGG 2 cut(s) 1326, 2543
PspN4I GGNNCC 8 cut(s) 565, 821, 1451, 1570, 1682, 1719, 2261, 2665
PspPI GGNCC 7 cut(s) 851, 1066, 1168, 1450, 1568, 1681, 2663
PspPPI RGGWCCY 1 cut(s) 1681
PsrI GAACNNNNNNTAC 2 cut(s) 1734, 1766
PstI CTGCAG 1 cut(s) 2049
PsuI RGATCY 1 cut(s) 2022
PteI GCGCGC 1 cut(s) 2685
RsaI GTAC 4 cut(s) 462, 605, 821, 2717
RsaNI GTAC 4 cut(s) 461, 604, 820, 2716
RseI CAYNNNNRTG 5 cut(s) 59, 90, 1802, 2265, 2739
SacI GAGCTC 2 cut(s) 255, 1563
SapI GCTCTTC 1 cut(s) 2823
SaqAI TTAA 5 cut(s) 419, 743, 1179, 1896, 2010
SatI GCNGC 3 cut(s) 1793, 2045, 2103
Sau3AI GATC 8 cut(s) 6, 2022, 2072, 2271, 2280, 2374, 2640, 2760
Sau96I GGNCC 7 cut(s) 851, 1066, 1168, 1450, 1568, 1681, 2663
ScaI AGTACT 1 cut(s) 605
SchI GAGTC 7 cut(s) 1157, 1254, 1263, 2341, 2524, 2534, 2876
ScrFI CCNGG 2 cut(s) 1328, 2545
SduI GDGCHC 5 cut(s) 255, 1563, 1876, 2634, 2713
SfaNI GCATC 5 cut(s) 222, 626, 1810, 2296, 2569
SfcI CTRYAG 1 cut(s) 2045
Sfr274I CTCGAG 1 cut(s) 878
SfuI TTCGAA 1 cut(s) 2055
SgrAI CRCCGGYG 1 cut(s) 776
SinI GGWCC 3 cut(s) 851, 1568, 1681
SlaI CTCGAG 1 cut(s) 878
SmiMI CAYNNNNRTG 5 cut(s) 59, 90, 1802, 2265, 2739
SmlI CTYRAG 4 cut(s) 455, 878, 1246, 2349
SmoI CTYRAG 4 cut(s) 455, 878, 1246, 2349
SphI GCATGC 1 cut(s) 58
SsiI CCGC 7 cut(s) 1220, 1630, 1721, 1793, 1857, 1880, 2523
SspMI CTAG 8 cut(s) 464, 573, 594, 1653, 1685, 2813, 2847, 2879
SstI GAGCTC 2 cut(s) 255, 1563
StyD4I CCNGG 2 cut(s) 1326, 2543
StyI CCWWGG 2 cut(s) 541, 1025
TaaI ACNGT 3 cut(s) 1108, 2715, 2868
TaiI ACGT 9 cut(s) 43, 118, 510, 527, 960, 1084, 1931, 2419, 2775
TatI WGTACW 1 cut(s) 603
TauI GCSGC 1 cut(s) 1795
TfiI GAWTC 8 cut(s) 928, 1359, 1645, 2057, 2083, 2159, 2198, 2550
Tru1I TTAA 5 cut(s) 419, 743, 1179, 1896, 2010
Tru9I TTAA 5 cut(s) 419, 743, 1179, 1896, 2010
TscAI CASTG 8 cut(s) 379, 1111, 1809, 1836, 2680, 2828, 2833, 2848
TseFI GTSAC 7 cut(s) 905, 1859, 1924, 2229, 2463, 2612, 2834
TseI GCWGC 2 cut(s) 2044, 2102
Tsp45I GTSAC 7 cut(s) 905, 1859, 1924, 2229, 2463, 2612, 2834
TspDTI ATGAA 8 cut(s) 74, 358, 677, 1581, 2075, 2136, 2542, 2877
TspGWI ACGGA 3 cut(s) 849, 2187, 2552
TspRI CASTG 8 cut(s) 379, 1111, 1809, 1836, 2680, 2828, 2833, 2848
Van91I CCANNNNNTGG 2 cut(s) 958, 1459
VpaK11BI GGWCC 3 cut(s) 851, 1568, 1681
XapI RAATTY 6 cut(s) 382, 639, 999, 1198, 1726, 2678
XceI RCATGY 5 cut(s) 58, 222, 367, 592, 1384
XcmI CCANNNNNNNNNTGG 1 cut(s) 2223
XhoI CTCGAG 1 cut(s) 878
XspI CTAG 8 cut(s) 464, 573, 594, 1653, 1685, 2813, 2847, 2879
ZrmI AGTACT 1 cut(s) 605
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.