pycom15g08560
NAC Family

Probably inactive leucine-rich repeat receptor-like protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0008421
Physical Location & Seq
Forward (+)
5400469 .. 5403557
3089 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom15g08560.1

Sequence Viewer

Length: 2838 bp
ATGATGATCAAGATGGAAAAGAGACAACATCATCAAACACGTCTCATATGGTGCATGCTCGTGTTCTTATTCTTCTTCTCAATTCATACTTGTGCTTTGGATGGTAGTGAGGAACGTCAGCTTCTCTTATCCTTCAAAGCTTCTGTCAACGACCCTTCACACTACCTCTCAAACTGGAACACTTCAGCCAACACTTTATGCAATTGGCATGGCATCACATGCAACGACAATAACAACATCAAAGCCATCGAGCTCACCGGAAGAAACATCTCCGGCAAGTTATCATCCTCCATCTTCCACTTGTCCCACATCGAAACCATCGACCTCTCCAACAATCAGCTATCCGGTCAACTCCCCAAAGACATGTTCATCAGCGGGTCCAATTCGCTTCGCCGTCTCAATTTCAGCAACAATATCTTAACCAGCACACTTCCACAAGGCTCACTCACGACCCTTGAGGTACTAGACTTGTCCAACAACATGATTTCAGGCATAATCCCAAACGACGTCGGATTGCTTTCTACCTTGAAGTTTCTTGACCTTGGAGGAAATATGTTGGTGGGAACCATCCCTAGCTCCATATCAAACATGTCTAGCTTGGAGTACTTGACTTTGGCATCAAACCAACTTGTTGGAAAAATTCCAACACAATTAGGCCAAATGAAGAACTTGAAGTGGATCTACTTGGGCTACAACAATCTCTCCGGCAATATACCGGAACAAATTGGTAACCTTTTTCTGTTAAATCATCTCAATCTAGTTTACAACAACCTCACCGGAGAAATCCCAATCACAATCAGCAACCTTACTAATCTTCGGTACCTTTTCCTCTACGGAAACAAACTCACAGGTCCAGTTCCACAATCCTTATTCAGTCTCGAGAAACTCGTTTCTCTCGACCTTAGTGACAATTTTCTTTCAGGAGAGATTCCCGAATGTGTTTTCAACCTCCAAAACGTGGAAATCCTCCATCTTTTTTCAAACAACTTCACGGGAAAAATTCCCAAAGCTCTGGCTTCTCTGCCAAGGCTTCAAGTCCTTCAGTTATGGTCAAACAAGTTCTCGGGCCAAATCCCAAAACGTCTCGGAAATCAAAACAGCCTCACTGTCTTAGACCTTTCCTCAAACAACCTTACTGGGAAAATACCCGATACGCTATGCGACTCGGGCCGGCTTTTTAAGCTCATCCTCTTCTCGAATTCACTCGAAGGCGCAATCCCGCTAAGCTTGTCTTCTTGCAAGAGCTTGAGCAGAGTCAGACTCCAGAACAACCGACTTTCAGGTGAATTATCGGCAGAGTTCACCAAACTCCCACTTGTCTATTTCCTGGATATCTCGGGCAACAATCTCTCTGGAAGAATCGACAACAGAAAGTGGGACATGCCATCTCTTCAAATGCTGAATATGGCGAGAAATCGATTTTTTGGGAAGTTACCGGAAACTTTCGGGAGCCAAAAGCTGGAGAACTTGGACTTGTCCGAAAATTGGTTCTCAGGAAGTATCTTGCCGAGTCTCGGGACCTTTCCGGAGCTAATGCAATTGAAGCTTTCTCACAATGAGCTCTCTGGTCCAATCCCTCAACAATTGTCTTCATGCAAAAAACTCGTTAGCCTCGACCTCAGTCACAACCGCCTCACCGGCACGATTCCCACTAGCCTCTCTGATATGCCGGTTCTTGGGGACCTAGATTTGTCCGAAAACCAAATATCGGGCGAGGTTCCGCGGAATTTGGGCGCTAAAGTATCGCTTGTTCAAGTCAACATTTCCCACAACAAACTCCACGGCATATTGCCGCCCACAGCAGTGTTTCTTGCCATAGATGCCAGCGCAGTGGACGGCAACAACCTCTGTGGTGGCAGTGACACTATGAGTGCTCTACCGCCGTGTAAGAGCGTTAAGAGAAACCCGACTTGGTGGTTTATTGTCACGTGTTTTCTTGTTGCATTATTGGCTTTTGGAGTTGCCAGTTATTTGTTTGTGCTATTGAGGAGGAGAAAGGAATTGGAGGTTAAATCGGTGGAGATCAAAGAAAGGATTTGGGAGCTGCAGTTTTTCGAATCAAAGGTTTCAAGATCAGTTACGATTCATGACATTTTATCGGCAGCCAAAGAAGGTAATATCATTGCCAAGGGTAAGACTGGAATTTCATACAAGGGAGAATCGGTTTCCAACGGAATGCAATTCGTGGTAAAAGAGGATTCTGTGAAATCACTTCAACCAAGCTTTTGGTCACAGATGGTTGAACTTGGGAAGCTTAGGCACCCCAATGTGATCAAACTGATCGGAATATGTCATTCCGAAGACGATGCGTATGTGCTTTTTGAGTATTGTGAAGGGAAAGTGTTGACTCAAGTTATGAGGGATTTGAGTTGGGATCAGCGCCGGAAAATTGCCGTTGGGATTGCGAAAGCTCTGCGTTTCTTGCATTGTTGTTGCTCGCCGAGCCTTGTAGCTGGTTGTATGTCACCAGAGAAAGTGATTGTAGACGCAAAAGGTGAGCCTCATATTCGATTGAGTCTCTCCGCACAAGTTCGGACGGACTCCAAGGGATTCATTGCTTCTGCATACATTGCTCCGGATGCTAAAGAAAGCAAAGTCTTTACAGAGAAGAGTGACATATATGGGTTTGGGCTCGTCTTGATCGAATTGCTGACCGGAAAAGGCCCCACCGACACTGAATTTGGCGCGCACCAGAGCGTCGTCGAATGGGCACGGTACTGCTACTCAGACTGCCATCTTGATGTCTGGACCGACTCGATCGATCAGAGAACACGTGTCGAGCAATCAGAACGAGATTGTGGAAACCATGAACCTAGCTCTTCACTGCACTGCTGGTGA

Protein Analysis

946

Amino Acids

104.97

Weight (kDa)

7.76

Isoelectric Point (pI)

36.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 37 - 75 1.3e-12 Leucine rich repeat N-terminal domain
LRR_14 PF23598 130 - 225 1.1e-07 Leucine-rich repeat region
LRR_8 PF13855 199 - 258 2.7e-06 Leucine rich repeat
LRR_8 PF13855 248 - 304 6.6e-07 Leucine rich repeat
LRR_14 PF23598 266 - 526 1.3e-10 Leucine-rich repeat region
LRR_8 PF13855 342 - 402 5e-07 Leucine rich repeat
LRR_8 PF13855 534 - 593 2.6e-07 Leucine rich repeat
Pkinase PF00069 705 - 894 2.5e-19 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 708 - 908 3.2e-17 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 510
Acc65I GGTACC 1 cut(s) 819
AccB1I GGYRCC 2 cut(s) 819, 2259
AccB7I CCANNNNNTGG 2 cut(s) 958, 1459
AccI GTMKAC 1 cut(s) 2484
AccII CGCG 2 cut(s) 1723, 2687
AccIII TCCGGA 2 cut(s) 1525, 2575
AciI CCGC 8 cut(s) 375, 1220, 1630, 1721, 1723, 1793, 1880, 2523
AclWI GGATC 2 cut(s) 686, 2382
AcsI RAATTY 6 cut(s) 639, 999, 1198, 1726, 2142, 2678
AcuI CTGAAG 2 cut(s) 168, 1025
AcvI CACGTG 2 cut(s) 1929, 2774
AcyI GRCGYC 1 cut(s) 507
AfaI GTAC 4 cut(s) 462, 605, 821, 2717
AfiI CCNNNNNNNGG 6 cut(s) 958, 1459, 1485, 1514, 1676, 1708
AflIII ACRYGT 6 cut(s) 38, 363, 588, 1928, 2771, 2773
AjiI CACGTC 1 cut(s) 41
AjnI CCWGG 1 cut(s) 1326
AjuI GAANNNNNNNTTGG 4 cut(s) 350, 382, 1894, 1926
AleI CACNNNNGTG 1 cut(s) 1802
Alw21I GWGCWC 3 cut(s) 255, 1563, 1876
Alw26I GTCTC 7 cut(s) 16, 47, 401, 881, 1088, 1517, 2522
AlwI GGATC 2 cut(s) 686, 2382
Ama87I CYCGRG 5 cut(s) 878, 1063, 1165, 1336, 1514
Aor13HI TCCGGA 2 cut(s) 1525, 2575
AoxI GGCC 4 cut(s) 655, 1066, 1168, 2662
ApeKI GCWGC 2 cut(s) 2044, 2102
ApoI RAATTY 6 cut(s) 639, 999, 1198, 1726, 2142, 2678
ArsI GACNNNNNNTTYG 4 cut(s) 129, 161, 2663, 2695
Asp718I GGTACC 1 cut(s) 819
AspLEI GCGC 6 cut(s) 1214, 1736, 1829, 2382, 2687, 2689
AspS9I GGNCC 9 cut(s) 378, 851, 1066, 1168, 1518, 1568, 1681, 2663, 2748
AsuHPI GGTGA 7 cut(s) 247, 766, 1294, 1295, 1627, 2457, 2507
AsuII TTCGAA 1 cut(s) 2055
AvaI CYCGRG 5 cut(s) 878, 1063, 1165, 1336, 1514
AvaII GGWCC 6 cut(s) 378, 851, 1518, 1568, 1681, 2748
BaeGI GKGCMC 1 cut(s) 2713
BanI GGYRCC 2 cut(s) 819, 2259
BanII GRGCYC 3 cut(s) 255, 1563, 2634
BarI GAAGNNNNNNTAC 2 cut(s) 665, 697
BauI CACGAG 1 cut(s) 59
BbrPI CACGTG 2 cut(s) 1929, 2774
BbsI GAAGAC 3 cut(s) 1224, 1581, 2307
Bbv12I GWGCWC 3 cut(s) 255, 1563, 1876
BbvI GCAGC 2 cut(s) 2031, 2114
BceAI ACGGC 5 cut(s) 378, 1798, 1852, 1867, 2378
BcgI CGANNNNNNTGC 6 cut(s) 1585, 1619, 2288, 2322, 2395, 2429
BciT130I CCWGG 1 cut(s) 1328
BclI TGATCA 2 cut(s) 6, 2271
BcoDI GTCTC 7 cut(s) 16, 47, 401, 881, 1088, 1517, 2522
BfaI CTAG 7 cut(s) 464, 573, 594, 758, 1653, 1685, 2814
BfmI CTRYAG 1 cut(s) 2045
BfoI RGCGCY 2 cut(s) 1737, 2383
BglI GCCNNNNNGGC 1 cut(s) 1638
BisI GCNGC 3 cut(s) 1793, 2045, 2103
BlpI GCTNAGC 1 cut(s) 1223
BlsI GCNGC 3 cut(s) 1794, 2046, 2104
BmcAI AGTACT 1 cut(s) 605
Bme1390I CCNGG 1 cut(s) 1328
Bme18I GGWCC 6 cut(s) 378, 851, 1518, 1568, 1681, 2748
BmeT110I CYCGRG 5 cut(s) 878, 1063, 1165, 1336, 1514
BmgBI CACGTC 1 cut(s) 41
BmgT120I GGNCC 9 cut(s) 378, 851, 1066, 1168, 1518, 1568, 1681, 2663, 2748
BmiI GGNNCC 9 cut(s) 379, 565, 821, 1451, 1519, 1682, 1719, 2261, 2665
BmrFI CCNGG 1 cut(s) 1328
BmrI ACTGGG 1 cut(s) 1146
BmsI GCATC 5 cut(s) 222, 626, 1810, 2296, 2569
BmuI ACTGGG 1 cut(s) 1146
BoxI GACNNNNGTC 1 cut(s) 1620
BpiI GAAGAC 3 cut(s) 1224, 1581, 2307
BplI GAGNNNNNCTC 2 cut(s) 1245, 1277
BpmI CTGGAG 2 cut(s) 1247, 1481
Bpu10I CCTNAGC 1 cut(s) 2255
Bpu1102I GCTNAGC 1 cut(s) 1223
Bpu14I TTCGAA 1 cut(s) 2055
BpuEI CTTGAG 3 cut(s) 476, 1267, 2334
Bsa29I ATCGAT 2 cut(s) 1417, 2760
BsaAI YACGTR 2 cut(s) 1929, 2774
BsaHI GRCGYC 1 cut(s) 507
BsaJI CCNNGG 6 cut(s) 541, 1025, 1721, 1780, 2127, 2544
BsaWI WCCGGW 8 cut(s) 257, 344, 715, 776, 1435, 1525, 2575, 2654
BsaXI ACNNNNNCTCC 6 cut(s) 686, 716, 2033, 2063, 2148, 2178
Bsc4I CCNNNNNNNGG 6 cut(s) 958, 1459, 1485, 1514, 1676, 1708
Bse118I RCCGGY 3 cut(s) 1170, 1637, 1669
Bse1I ACTGG 5 cut(s) 179, 854, 1141, 1965, 2143
Bse3DI GCAATG 3 cut(s) 2121, 2553, 2568
BseAI TCCGGA 2 cut(s) 1525, 2575
BseBI CCWGG 1 cut(s) 1328
BseCI ATCGAT 2 cut(s) 1417, 2760
BseDI CCNNGG 6 cut(s) 541, 1025, 1721, 1780, 2127, 2544
BseGI GGATG 5 cut(s) 106, 284, 567, 1185, 2584
BseLI CCNNNNNNNGG 6 cut(s) 958, 1459, 1485, 1514, 1676, 1708
BseMI GCAATG 3 cut(s) 2121, 2553, 2568
BseMII CTCAG 3 cut(s) 1506, 1633, 2739
BseNI ACTGG 5 cut(s) 179, 854, 1141, 1965, 2143
BsePI GCGCGC 1 cut(s) 2685
BseRI GAGGAG 2 cut(s) 2002, 2005
BseSI GKGCMC 1 cut(s) 2713
BseXI GCAGC 2 cut(s) 2031, 2114
BsgI GTGCAG 1 cut(s) 2810
Bsh1236I CGCG 2 cut(s) 1723, 2687
Bsh1285I CGRYCG 1 cut(s) 2760
BshFI GGCC 4 cut(s) 657, 1068, 1170, 2664
BshNI GGYRCC 2 cut(s) 819, 2259
BshVI ATCGAT 2 cut(s) 1417, 2760
BsiEI CGRYCG 1 cut(s) 2760
BsiHKAI GWGCWC 3 cut(s) 255, 1563, 1876
BsiHKCI CYCGRG 5 cut(s) 878, 1063, 1165, 1336, 1514
BslFI GGGAC 4 cut(s) 289, 1391, 1531, 1694
BslI CCNNNNNNNGG 6 cut(s) 958, 1459, 1485, 1514, 1676, 1708
BsmAI GTCTC 7 cut(s) 16, 47, 401, 881, 1088, 1517, 2522
BsmBI CGTCTC 3 cut(s) 47, 401, 1088
BsmFI GGGAC 4 cut(s) 289, 1391, 1531, 1694
BsmI GAATGC 1 cut(s) 2181
BsnI GGCC 4 cut(s) 657, 1068, 1170, 2664
BsoBI CYCGRG 5 cut(s) 878, 1063, 1165, 1336, 1514
Bsp119I TTCGAA 1 cut(s) 2055
Bsp1286I GDGCHC 5 cut(s) 255, 1563, 1876, 2634, 2713
Bsp13I TCCGGA 2 cut(s) 1525, 2575
Bsp1720I GCTNAGC 1 cut(s) 1223
BspACI CCGC 8 cut(s) 375, 1220, 1630, 1721, 1723, 1793, 1880, 2523
BspANI GGCC 4 cut(s) 657, 1068, 1170, 2664
BspCNI CTCAG 3 cut(s) 1505, 1632, 2738
BspDI ATCGAT 2 cut(s) 1417, 2760
BspEI TCCGGA 2 cut(s) 1525, 2575
BspFNI CGCG 2 cut(s) 1723, 2687
BspHI TCATGA 1 cut(s) 2086
BspLI GGNNCC 9 cut(s) 379, 565, 821, 1451, 1519, 1682, 1719, 2261, 2665
BspMAI CTGCAG 1 cut(s) 2049
BspPI GGATC 2 cut(s) 686, 2382
BspQI GCTCTTC 1 cut(s) 2824
BspT104I TTCGAA 1 cut(s) 2055
BspT107I GGYRCC 2 cut(s) 819, 2259
BsrDI GCAATG 3 cut(s) 2121, 2553, 2568
BsrFI RCCGGY 3 cut(s) 1170, 1637, 1669
BsrI ACTGG 5 cut(s) 179, 854, 1141, 1965, 2143
BssAI RCCGGY 3 cut(s) 1170, 1637, 1669
BssECI CCNNGG 6 cut(s) 541, 1025, 1721, 1780, 2127, 2544
BssHII GCGCGC 1 cut(s) 2685
BssNI GRCGYC 1 cut(s) 507
BssSI CACGAG 1 cut(s) 59
BssT1I CCWWGG 4 cut(s) 541, 1025, 2127, 2544
Bst2BI CACGAG 1 cut(s) 59
Bst2UI CCWGG 1 cut(s) 1328
Bst4CI ACNGT 2 cut(s) 1108, 2715
Bst6I CTCTTC 4 cut(s) 1196, 1395, 2603, 2824
BstACI GRCGYC 1 cut(s) 507
BstAPI GCANNNNNTGC 2 cut(s) 219, 2570
BstBAI YACGTR 2 cut(s) 1929, 2774
BstBI TTCGAA 1 cut(s) 2055
BstC8I GCNNGC 5 cut(s) 56, 1172, 1825, 2438, 2687
BstDEI CTNAG 7 cut(s) 902, 1111, 1223, 1492, 1619, 2255, 2725
BstDSI CCRYGG 2 cut(s) 1721, 1780
BstEII GGTNACC 1 cut(s) 728
BstF5I GGATG 5 cut(s) 106, 284, 567, 1185, 2584
BstFNI CGCG 2 cut(s) 1723, 2687
BstH2I RGCGCY 2 cut(s) 1737, 2383
BstHHI GCGC 6 cut(s) 1214, 1736, 1829, 2382, 2687, 2689
BstMAI GTCTC 7 cut(s) 16, 47, 401, 881, 1088, 1517, 2522
BstMCI CGRYCG 1 cut(s) 2760
BstNI CCWGG 1 cut(s) 1328
BstNSI RCATGY 5 cut(s) 58, 222, 367, 592, 1384
BstPAI GACNNNNGTC 1 cut(s) 1620
BstPI GGTNACC 1 cut(s) 728
BstSCI CCNGG 1 cut(s) 1326
BstSFI CTRYAG 1 cut(s) 2045
BstSLI GKGCMC 1 cut(s) 2713
BstUI CGCG 2 cut(s) 1723, 2687
BstV1I GCAGC 2 cut(s) 2031, 2114
BstV2I GAAGAC 3 cut(s) 1224, 1581, 2307
BstX2I RGATCY 1 cut(s) 678
BstXI CCANNNNNNTGG 4 cut(s) 632, 1012, 1831, 2226
BstYI RGATCY 1 cut(s) 678
Bsu15I ATCGAT 2 cut(s) 1417, 2760
BsuRI GGCC 4 cut(s) 657, 1068, 1170, 2664
BsuTUI ATCGAT 2 cut(s) 1417, 2760
BtgI CCRYGG 2 cut(s) 1721, 1780
BtrI CACGTC 1 cut(s) 41
BtsCI GGATG 5 cut(s) 106, 284, 567, 1185, 2584
BtsI GCAGTG 5 cut(s) 1809, 1836, 1864, 2822, 2827
BtsIMutI CAGTG 7 cut(s) 1104, 1809, 1836, 1864, 2673, 2822, 2827
Cac8I GCNNGC 5 cut(s) 56, 1172, 1825, 2438, 2687
CciI TCATGA 1 cut(s) 2086
CfoI GCGC 6 cut(s) 1214, 1736, 1829, 2382, 2687, 2689
Cfr10I RCCGGY 3 cut(s) 1170, 1637, 1669
Cfr13I GGNCC 9 cut(s) 378, 851, 1066, 1168, 1518, 1568, 1681, 2663, 2748
Cfr42I CCGCGG 1 cut(s) 1724
ClaI ATCGAT 2 cut(s) 1417, 2760
CseI GACGC 2 cut(s) 2495, 2686
Csp6I GTAC 4 cut(s) 461, 604, 820, 2716
CspCI CAANNNNNGTGG 2 cut(s) 1831, 1866
CviQI GTAC 4 cut(s) 461, 604, 820, 2716
DdeI CTNAG 7 cut(s) 902, 1111, 1223, 1492, 1619, 2255, 2725
Eam1104I CTCTTC 4 cut(s) 1196, 1395, 2603, 2824
EarI CTCTTC 4 cut(s) 1196, 1395, 2603, 2824
Ecl136II GAGCTC 2 cut(s) 253, 1561
Eco130I CCWWGG 4 cut(s) 541, 1025, 2127, 2544
Eco24I GRGCYC 3 cut(s) 255, 1563, 2634
Eco32I GATATC 1 cut(s) 1333
Eco47I GGWCC 6 cut(s) 378, 851, 1518, 1568, 1681, 2748
Eco53kI GAGCTC 2 cut(s) 253, 1561
Eco57I CTGAAG 2 cut(s) 168, 1025
Eco72I CACGTG 2 cut(s) 1929, 2774
Eco88I CYCGRG 5 cut(s) 878, 1063, 1165, 1336, 1514
Eco91I GGTNACC 1 cut(s) 728
EcoICRI GAGCTC 2 cut(s) 253, 1561
EcoO109I RGGNCCY 3 cut(s) 1518, 1681, 2663
EcoO65I GGTNACC 1 cut(s) 728
EcoRI GAATTC 1 cut(s) 1198
EcoRII CCWGG 1 cut(s) 1326
EcoRV GATATC 1 cut(s) 1333
EcoT14I CCWWGG 4 cut(s) 541, 1025, 2127, 2544
EcoT38I GRGCYC 3 cut(s) 255, 1563, 2634
ErhI CCWWGG 4 cut(s) 541, 1025, 2127, 2544
Esp3I CGTCTC 3 cut(s) 47, 401, 1088
FalI AAGNNNNNCTT 4 cut(s) 1216, 1248, 1731, 1763
FaqI GGGAC 4 cut(s) 289, 1391, 1531, 1694
FauI CCCGC 2 cut(s) 368, 1227
FauNDI CATATG 1 cut(s) 47
FbaI TGATCA 2 cut(s) 6, 2271
FblI GTMKAC 1 cut(s) 2484
Fnu4HI GCNGC 3 cut(s) 1793, 2045, 2103
FokI GGATG 5 cut(s) 113, 271, 554, 1172, 2591
FriOI GRGCYC 3 cut(s) 255, 1563, 2634
Fsp4HI GCNGC 3 cut(s) 1793, 2045, 2103
FspBI CTAG 7 cut(s) 464, 573, 594, 758, 1653, 1685, 2814
GlaI GCGC 6 cut(s) 1213, 1735, 1828, 2381, 2686, 2688
GluI GCNGC 3 cut(s) 1793, 2045, 2103
GsuI CTGGAG 2 cut(s) 1247, 1481
HaeII RGCGCY 2 cut(s) 1737, 2383
HaeIII GGCC 4 cut(s) 657, 1068, 1170, 2664
HgaI GACGC 2 cut(s) 2495, 2686
HhaI GCGC 6 cut(s) 1214, 1736, 1829, 2382, 2687, 2689
Hin1I GRCGYC 1 cut(s) 507
Hin6I GCGC 6 cut(s) 1212, 1734, 1827, 2380, 2685, 2687
HinP1I GCGC 6 cut(s) 1212, 1734, 1827, 2380, 2685, 2687
HincII GTYRAC 4 cut(s) 148, 350, 1759, 2346
HindII GTYRAC 4 cut(s) 148, 350, 1759, 2346
HindIII AAGCTT 5 cut(s) 138, 1225, 1544, 2221, 2252
HphI GGTGA 7 cut(s) 247, 766, 1294, 1295, 1627, 2457, 2507
Hpy166II GTNNAC 8 cut(s) 148, 350, 763, 1302, 1759, 1834, 2346, 2485
Hpy8I GTNNAC 8 cut(s) 148, 350, 763, 1302, 1759, 1834, 2346, 2485
Hpy99I CGWCG 4 cut(s) 509, 512, 2702, 2705
HpyAV CCTTC 6 cut(s) 142, 165, 1049, 1202, 2105, 2327
HpyCH4III ACNGT 2 cut(s) 1108, 2715
HpyCH4IV ACGT 7 cut(s) 40, 115, 507, 957, 1081, 1928, 2773
HpyF3I CTNAG 7 cut(s) 902, 1111, 1223, 1492, 1619, 2255, 2725
HpySE526I ACGT 7 cut(s) 40, 115, 507, 957, 1081, 1928, 2773
Hsp92I GRCGYC 1 cut(s) 507
HspAI GCGC 6 cut(s) 1212, 1734, 1827, 2380, 2685, 2687
Kpn2I TCCGGA 2 cut(s) 1525, 2575
KpnI GGTACC 1 cut(s) 823
KroI GCCGGC 1 cut(s) 1170
KroNI GCCGGC 1 cut(s) 1172
Ksp22I TGATCA 2 cut(s) 6, 2271
KspI CCGCGG 1 cut(s) 1724
LguI GCTCTTC 1 cut(s) 2824
LmnI GCTCC 5 cut(s) 581, 1449, 1528, 2041, 2578
Lsp1109I GCAGC 2 cut(s) 2031, 2114
LweI GCATC 5 cut(s) 222, 626, 1810, 2296, 2569
MaeI CTAG 7 cut(s) 464, 573, 594, 758, 1653, 1685, 2814
MaeII ACGT 7 cut(s) 40, 115, 507, 957, 1081, 1928, 2773
MfeI CAATTG 3 cut(s) 202, 1538, 1583
MflI RGATCY 1 cut(s) 678
MhlI GDGCHC 5 cut(s) 255, 1563, 1876, 2634, 2713
MlyI GAGTC 8 cut(s) 1157, 1254, 1263, 1519, 2341, 2524, 2534, 2747
MmeI TCCRAC 6 cut(s) 354, 490, 498, 613, 668, 2193
MroI TCCGGA 2 cut(s) 1525, 2575
MroNI GCCGGC 1 cut(s) 1170
MseI TTAA 5 cut(s) 419, 743, 1179, 1896, 2010
MslI CAYNNNNRTG 5 cut(s) 59, 90, 1802, 2265, 2739
MspA1I CMGCKG 2 cut(s) 375, 1723
MspR9I CCNGG 1 cut(s) 1328
MunI CAATTG 3 cut(s) 202, 1538, 1583
Mva1269I GAATGC 1 cut(s) 2181
MvaI CCWGG 1 cut(s) 1328
MvnI CGCG 2 cut(s) 1723, 2687
NaeI GCCGGC 1 cut(s) 1172
NdeI CATATG 1 cut(s) 47
NgoMIV GCCGGC 1 cut(s) 1170
NlaIV GGNNCC 9 cut(s) 379, 565, 821, 1451, 1519, 1682, 1719, 2261, 2665
NmeAIII GCCGAG 2 cut(s) 1533, 2466
NmuCI GTSAC 7 cut(s) 905, 1622, 1859, 1924, 2229, 2463, 2612
NspI RCATGY 5 cut(s) 58, 222, 367, 592, 1384
NspV TTCGAA 1 cut(s) 2055
OliI CACNNNNGTG 1 cut(s) 1802
PaeI GCATGC 1 cut(s) 58
PaeR7I CTCGAG 1 cut(s) 878
PagI TCATGA 1 cut(s) 2086
PauI GCGCGC 1 cut(s) 2685
PciI ACATGT 2 cut(s) 363, 588
PciSI GCTCTTC 1 cut(s) 2824
PcsI WCGNNNNNNNCGW 5 cut(s) 318, 885, 894, 1611, 2640
PctI GAATGC 1 cut(s) 2181
PdiI GCCGGC 1 cut(s) 1172
PfeI GAWTC 8 cut(s) 928, 1359, 1645, 2057, 2083, 2159, 2198, 2550
PflMI CCANNNNNTGG 2 cut(s) 958, 1459
PfoI TCCNGGA 1 cut(s) 1326
PkrI GCNGC 3 cut(s) 1794, 2046, 2104
Ple19I CGATCG 1 cut(s) 2760
PleI GAGTC 8 cut(s) 1157, 1254, 1262, 1518, 2341, 2523, 2534, 2747
PmaCI CACGTG 2 cut(s) 1929, 2774
PmlI CACGTG 2 cut(s) 1929, 2774
PpsI GAGTC 8 cut(s) 1157, 1254, 1262, 1518, 2341, 2523, 2534, 2747
Ppu21I YACGTR 2 cut(s) 1929, 2774
PpuMI RGGWCCY 2 cut(s) 1518, 1681
PscI ACATGT 2 cut(s) 363, 588
PshAI GACNNNNGTC 1 cut(s) 1620
Psp124BI GAGCTC 2 cut(s) 255, 1563
Psp5II RGGWCCY 2 cut(s) 1518, 1681
Psp6I CCWGG 1 cut(s) 1326
PspCI CACGTG 2 cut(s) 1929, 2774
PspEI GGTNACC 1 cut(s) 728
PspGI CCWGG 1 cut(s) 1326
PspN4I GGNNCC 9 cut(s) 379, 565, 821, 1451, 1519, 1682, 1719, 2261, 2665
PspPI GGNCC 9 cut(s) 378, 851, 1066, 1168, 1518, 1568, 1681, 2663, 2748
PspPPI RGGWCCY 2 cut(s) 1518, 1681
PsrI GAACNNNNNNTAC 2 cut(s) 1734, 1766
PstI CTGCAG 1 cut(s) 2049
PsuI RGATCY 1 cut(s) 678
PteI GCGCGC 1 cut(s) 2685
PvuI CGATCG 1 cut(s) 2760
RsaI GTAC 4 cut(s) 462, 605, 821, 2717
RsaNI GTAC 4 cut(s) 461, 604, 820, 2716
RseI CAYNNNNRTG 5 cut(s) 59, 90, 1802, 2265, 2739
SacI GAGCTC 2 cut(s) 255, 1563
SacII CCGCGG 1 cut(s) 1724
SapI GCTCTTC 1 cut(s) 2824
SaqAI TTAA 5 cut(s) 419, 743, 1179, 1896, 2010
SatI GCNGC 3 cut(s) 1793, 2045, 2103
Sau96I GGNCC 9 cut(s) 378, 851, 1066, 1168, 1518, 1568, 1681, 2663, 2748
ScaI AGTACT 1 cut(s) 605
SchI GAGTC 8 cut(s) 1157, 1254, 1263, 1519, 2341, 2524, 2534, 2747
ScrFI CCNGG 1 cut(s) 1328
SduI GDGCHC 5 cut(s) 255, 1563, 1876, 2634, 2713
SfaNI GCATC 5 cut(s) 222, 626, 1810, 2296, 2569
SfcI CTRYAG 1 cut(s) 2045
Sfr274I CTCGAG 1 cut(s) 878
Sfr303I CCGCGG 1 cut(s) 1724
SfuI TTCGAA 1 cut(s) 2055
SgrBI CCGCGG 1 cut(s) 1724
SinI GGWCC 6 cut(s) 378, 851, 1518, 1568, 1681, 2748
SlaI CTCGAG 1 cut(s) 878
SmiMI CAYNNNNRTG 5 cut(s) 59, 90, 1802, 2265, 2739
SmlI CTYRAG 4 cut(s) 455, 878, 1246, 2349
SmoI CTYRAG 4 cut(s) 455, 878, 1246, 2349
SphI GCATGC 1 cut(s) 58
SsiI CCGC 8 cut(s) 375, 1220, 1630, 1721, 1723, 1793, 1880, 2523
SspMI CTAG 7 cut(s) 464, 573, 594, 758, 1653, 1685, 2814
SstI GAGCTC 2 cut(s) 255, 1563
StyD4I CCNGG 1 cut(s) 1326
StyI CCWWGG 4 cut(s) 541, 1025, 2127, 2544
TaaI ACNGT 2 cut(s) 1108, 2715
TaiI ACGT 7 cut(s) 43, 118, 510, 960, 1084, 1931, 2776
TaqII GACCGA 1 cut(s) 2765
TatI WGTACW 1 cut(s) 603
TauI GCSGC 1 cut(s) 1795
TfiI GAWTC 8 cut(s) 928, 1359, 1645, 2057, 2083, 2159, 2198, 2550
Tru1I TTAA 5 cut(s) 419, 743, 1179, 1896, 2010
Tru9I TTAA 5 cut(s) 419, 743, 1179, 1896, 2010
TscAI CASTG 7 cut(s) 1111, 1809, 1836, 1864, 2680, 2829, 2834
TseFI GTSAC 7 cut(s) 905, 1622, 1859, 1924, 2229, 2463, 2612
TseI GCWGC 2 cut(s) 2044, 2102
Tsp45I GTSAC 7 cut(s) 905, 1622, 1859, 1924, 2229, 2463, 2612
TspDTI ATGAA 8 cut(s) 74, 358, 677, 1581, 2075, 2136, 2542, 2823
TspGWI ACGGA 3 cut(s) 849, 2187, 2552
TspRI CASTG 7 cut(s) 1111, 1809, 1836, 1864, 2680, 2829, 2834
Van91I CCANNNNNTGG 2 cut(s) 958, 1459
VpaK11BI GGWCC 6 cut(s) 378, 851, 1518, 1568, 1681, 2748
XapI RAATTY 6 cut(s) 639, 999, 1198, 1726, 2142, 2678
XceI RCATGY 5 cut(s) 58, 222, 367, 592, 1384
XhoI CTCGAG 1 cut(s) 878
XmiI GTMKAC 1 cut(s) 2484
XspI CTAG 7 cut(s) 464, 573, 594, 758, 1653, 1685, 2814
ZraI GACGTC 1 cut(s) 508
ZrmI AGTACT 1 cut(s) 605
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.