Rorug06G0350400
NAC Family

Probably inactive leucine-rich repeat receptor-like protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0008421
Physical Location & Seq
Forward (+)
49972059 .. 49977419
5361 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0350400.1

Sequence Viewer

Length: 2847 bp
ATGGAGGCGACTACTCCTCCGTCCGATTCTCCATCTATAAAACCCATAAACAAGTCAGTGGTCCACAGAATCTGCGCCGGCCAAGTGATCCTCGACCTCTCGGCCGCCGTCAAAGAATTGGTCGAGAACAGCCTCGACGCCGGCGCCACCACCATCGAGATCGCGCTCAAAGACTACGGCAAAGAGTGGTTCCAGGTCATCGATAACGGCTGCGGCATTTCGCCCAACAACTTCAAGGTCCTTGCCCTCAAGCACCACACTTCCAAACTCGCCGGGTTCCCTGACCTCCAGTCTCTGACGACGTTCGGCTTCCGCGGCGAGGCCTTGAGCTCTCTGTGTGCTTTGGGGAATTTAACGGTGGAAACCAGGACCAAGTACGAGCAGGTCGCCACGCACTTGACTTTTGACCATTCCGGTGTGTTGGTAACGGAAAAGAAGACGGCGCGGCAGGTCGGCACCACCGTAACGGTCAAGAATCTGTTTGTGAATTTACCGGTGAGGTGCAAGGAGTTCAGTCGCAACATTCGGAAGGAGTATGGGAAGCTGGTTTCTCTATTGAATGCCTATGCTCTTATTGCGAAAGGGGTTCGGTTAGTCTGCACGAATGCCATAGGTAGAAATGCAAAGTCTGTGGTGCTTAAAACGCAAGGAAGTGGTTCACTTAAGGATAATATTGTGACATTGTTTGGGATGAGCACTTTCAGTTGTTTAGAGCCTGTGAGTATATCTGTATCTGATAGTTGCAAGGTTGATGGTTTCCTTTCCAAGTCGGGACAAGGTAGTGGTCGGAACATGGGAGATAGGCAGTTCTTTTTCGTGAATGGTCGACCTGTGGATATGCCTAAAATTACAAAGCTTGTGAATGAGTTGTATAGAGGGGCAAACTCCCAGCAGCATCCTATTGCCATATTGAATTTCACTGTTCCGACAAGAGCGTGTGATGTCAATGTTACTCCTGATAAAAGGAAAGTGTTCTTTTCTGATGAGAGCTCCATACTTGTTGCCTTAAGGGAGGGTCTGCAGCAGATATATTCCACTAGTAATGCTCGTTACTCTGTTAATAAAGTGGAGGAGCCTGCCAAAGAAGCAGGCAGGTCTCAGTTGTGTTCACCTCATCAAAGGTCTCATAAACAATTAGCTAGGGATGATGATGATGATGATGTTCCTGAAGAAGTTCGTCCGGCTGATAAAGCTGAGGAGGCTACAAAAGAAGGAGGCAGGTCTGAATTGTGTTTTCCTGGTCAGAGGTCTCATATGTTTCTGAGACAATCCTCTATAGATTCTGTACCTAAAGAAGTTAGTCCTGAGGATCACACTCCAGAAGGCGATGCTCCTCTGAAAGCTGCGGAAACTGATTCTGAAACTAATCATGATGAGGAAGGGTTTAGCCATGAAAATTCAATGAGGAAGGATTTTGCTTTAAGAGTACACAGCATTAAGAAGGTCCATGGAACTAGTCAATTGACAAAGAATCTCAGTAGTATGAAAGCTGATCGAATTGCAGCAAATGGAGATTCATGTAGTCATTCAAGCTCTGTTCAAGCATCAATTAACCAGTTTGTAACTGTAACTAAAAGAAAACATGATAGTATTAGCACAGTCCTGTCTGAAATGCCTGTCCTAAGGAACCAAAGTCTTCATTGTCAATCCAAGAATGATTCACTTAATGGTGTTTCAAAACCCCCACTTAGTCATCAGCAGATCAATGATTCTACTGAAGTTGATAATTCTGCTGAAGCTGGTGAGAATGAGCCATCCAAATATTTTAGAGTAGATAGGATCCTCAACAAAATTAGAGTTCCAGTTTCTCCTGGAGGCAAGATTGAGGGTGAAGGACATGGAGAAGGTTTACAAGCTCAACAGAAAGCAGTGCCTGTTGCTGATATGGCACCAACTGCTTCACCCAGCAGGGATATAAGTCTGTCTGAAGATCTGCCAGCTGCATCACCTTCTTCTTGTATATTGTCGAATACTCCAAAGCCTTCTTCTGGTCTTATGATGTGTTCGACCTTGAGATTTAGTTTCCAGGACCTAAAGACAAGGAGGCAGCAAATATATTCTAGATTGCAATCAAGCATACCTGGAGTAAAAGCACAAAGGTGCTATACTGCTGCAACATTGGAGCTTTCTCAACCAGAAAATGAGGAGCGGAAAGCAAGGGCCTTAGCTGCAGCTACCACGGAGTTGGAAAGACTTTTCAGAAAAGAAGATTTTGGTAGAATGAAGGTGATTGGGCAATTCAATCTTGGCTTCATTATTGGGAAATTAGATCAAGATTTATTTATCGTAGATCAGCATGCAGCTGATGAGAAGTACAATTTTGAGCGCCTGTCACAATCTACCATCTTAAACCAACAGCCTTTGCTTAGGCCATTGAGGTTGGAGTTATCTCCTGAAGAAGAAGTTGTTGCTTCAATGCACATGGATGTAATCAGGAAAAATGGATTTTCTTTGGAAGAGGATCTACATGCTCCGCCTGGTCACCATTTTAAATTGAAAGCTGTTCCCTTCAGTAAAAACATAACTTTTGGAGTTGAAGATGTGAAGGATTTGATATCCACTCTTGCTGATAGTCATGGGGAATGCTCAATCATCGGTAGTTACAAGATGGACACTGTTGACTCCGTTTGCCCTTCTAGAGTTCGCGCGATGCTGGCATCACGTGCATGCAGATCATCTGTTATGATTGGAGATGCCCTCGGAAGAAATGAGATGCGGAAGATTCTTGAGCATCTGGCAGGTCTGAAGTCTCCTTGGAATTGCCCTCATGGCAGGCCAACCATGCGACATTTGATTGATTTGAAAACCATACACAGGTCTGAAGAAAACGATGATGCAGAGTCTTGA

Protein Analysis

948

Amino Acids

104.32

Weight (kDa)

7.35

Isoelectric Point (pI)

49.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HATPase_c PF02518 31 - 87 2.3e-06 Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase
HATPase_c_3 PF13589 34 - 136 4.1e-15 Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase
DNA_mis_repair PF01119 227 - 344 7.1e-25 DNA mismatch repair protein, C-terminal domain
MutL_C PF08676 744 - 900 2e-25 MutL C terminal dimerisation domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 5 cut(s) 373, 439, 1083, 1209, 2729
AccB1I GGYRCC 3 cut(s) 143, 455, 1888
AccBSI CCGCTC 1 cut(s) 2149
AccI GTMKAC 1 cut(s) 826
AccII CGCG 5 cut(s) 164, 315, 445, 2646, 2648
AciI CCGC 9 cut(s) 105, 213, 313, 315, 445, 1346, 2149, 2474, 2716
AclWI GGATC 5 cut(s) 82, 1317, 1774, 1787, 2469
AcoI YGGCCR 2 cut(s) 79, 102
AcsI RAATTY 4 cut(s) 349, 487, 913, 1396
AcuI CTGAAG 8 cut(s) 1188, 1737, 1755, 1947, 2415, 2494, 2765, 2841
AcvI CACGTG 1 cut(s) 2663
AcyI GRCGYC 2 cut(s) 138, 144
AfaI GTAC 4 cut(s) 377, 1287, 1428, 2315
AfiI CCNNNNNNNGG 3 cut(s) 319, 1988, 2814
AflII CTTAAG 2 cut(s) 662, 1006
AgeI ACCGGT 1 cut(s) 493
AhdI GACNNNNNGTC 1 cut(s) 289
AhlI ACTAGT 2 cut(s) 1037, 1454
AjnI CCWGG 7 cut(s) 192, 365, 1237, 1810, 2025, 2080, 2476
AjuI GAANNNNNNNTTGG 2 cut(s) 1969, 2001
AleI CACNNNNGTG 1 cut(s) 2098
Alw21I GWGCWC 3 cut(s) 332, 698, 992
Alw26I GTCTC 6 cut(s) 297, 1101, 1128, 1254, 1258, 2754
AlwI GGATC 5 cut(s) 82, 1317, 1774, 1787, 2469
AlwNI CAGNNNCTG 3 cut(s) 72, 295, 1874
AoxI GGCC 6 cut(s) 79, 102, 321, 2160, 2369, 2774
ApoI RAATTY 4 cut(s) 349, 487, 913, 1396
ArsI GACNNNNNNTTYG 2 cut(s) 105, 137
AsiGI ACCGGT 1 cut(s) 493
Asp700I GAANNNNTTC 3 cut(s) 655, 971, 1173
AspLEI GCGC 6 cut(s) 77, 146, 166, 445, 2328, 2648
AspS9I GGNCC 6 cut(s) 61, 238, 369, 1444, 2029, 2160
AsuC2I CCSGG 1 cut(s) 274
AsuHPI GGTGA 8 cut(s) 508, 1101, 1754, 1841, 1893, 1938, 2239, 2474
AvaII GGWCC 5 cut(s) 61, 238, 369, 1444, 2029
AxyI CCTNAGG 2 cut(s) 1305, 1622
BamHI GGATCC 1 cut(s) 1779
BanI GGYRCC 3 cut(s) 143, 455, 1888
BanII GRGCYC 2 cut(s) 332, 992
BarI GAAGNNNNNNTAC 2 cut(s) 2448, 2480
BbrPI CACGTG 1 cut(s) 2663
BbsI GAAGAC 2 cut(s) 443, 1628
Bbv12I GWGCWC 3 cut(s) 332, 698, 992
BbvCI CCTCAGC 1 cut(s) 1194
BccI CCATC 6 cut(s) 40, 161, 746, 1762, 2351, 2602
BceAI ACGGC 4 cut(s) 92, 193, 223, 456
BciT130I CCWGG 7 cut(s) 194, 367, 1239, 1812, 2027, 2082, 2478
BcnI CCSGG 1 cut(s) 274
BcoDI GTCTC 6 cut(s) 297, 1101, 1128, 1254, 1258, 2754
BcuI ACTAGT 2 cut(s) 1037, 1454
BfaI CTAG 5 cut(s) 1038, 1140, 1455, 2061, 2637
BfmI CTRYAG 3 cut(s) 1019, 1275, 2169
BfoI RGCGCY 2 cut(s) 147, 2329
BfrI CTTAAG 2 cut(s) 662, 1006
BfuAI ACCTGC 5 cut(s) 373, 439, 1083, 1209, 2729
BglI GCCNNNNNGGC 1 cut(s) 2769
BglII AGATCT 1 cut(s) 1930
Bme1390I CCNGG 8 cut(s) 194, 274, 367, 1239, 1812, 2027, 2082, 2478
Bme18I GGWCC 5 cut(s) 61, 238, 369, 1444, 2029
BmeRI GACNNNNNGTC 1 cut(s) 289
BmgT120I GGNCC 6 cut(s) 61, 238, 369, 1444, 2029, 2160
BmiI GGNNCC 8 cut(s) 145, 191, 278, 457, 1074, 1628, 1781, 1890
BmrFI CCNGG 8 cut(s) 194, 274, 367, 1239, 1812, 2027, 2082, 2478
BpiI GAAGAC 2 cut(s) 443, 1628
BplI GAGNNNNNCTC 2 cut(s) 2682, 2714
BpmI CTGGAG 4 cut(s) 272, 1302, 1833, 2103
Bpu10I CCTNAGC 3 cut(s) 1194, 2164, 2366
BpuEI CTTGAG 4 cut(s) 233, 346, 2032, 2747
BpuMI CCSGG 1 cut(s) 274
Bsa29I ATCGAT 1 cut(s) 201
BsaAI YACGTR 1 cut(s) 2663
BsaBI GATNNNNATC 1 cut(s) 2068
BsaHI GRCGYC 2 cut(s) 138, 144
BsaI GGTCTC 3 cut(s) 1101, 1128, 1254
BsaJI CCNNGG 5 cut(s) 313, 1447, 2178, 2698, 2753
BsaWI WCCGGW 2 cut(s) 413, 493
BsaXI ACNNNNNCTCC 1 cut(s) 31
Bsc4I CCNNNNNNNGG 3 cut(s) 319, 1988, 2814
Bse118I RCCGGY 3 cut(s) 77, 140, 493
Bse1I ACTGG 3 cut(s) 289, 1555, 1802
Bse21I CCTNAGG 2 cut(s) 1305, 1622
Bse8I GATNNNNATC 1 cut(s) 2068
BseBI CCWGG 7 cut(s) 194, 367, 1239, 1812, 2027, 2082, 2478
BseCI ATCGAT 1 cut(s) 201
BseDI CCNNGG 5 cut(s) 313, 1447, 2178, 2698, 2753
BseGI GGATG 5 cut(s) 696, 895, 1150, 1754, 2431
BseJI GATNNNNATC 1 cut(s) 2068
BseLI CCNNNNNNNGG 3 cut(s) 319, 1988, 2814
BseMII CTCAG 5 cut(s) 1112, 1185, 1253, 1296, 1489
BseNI ACTGG 3 cut(s) 289, 1555, 1802
BseRI GAGGAG 5 cut(s) 6, 1085, 1211, 1323, 2159
BseX3I CGGCCG 1 cut(s) 102
BseYI CCCAGC 2 cut(s) 888, 1904
BsgI GTGCAG 1 cut(s) 583
Bsh1236I CGCG 5 cut(s) 164, 315, 445, 2646, 2648
Bsh1285I CGRYCG 1 cut(s) 105
BshFI GGCC 6 cut(s) 81, 104, 323, 2162, 2371, 2776
BshNI GGYRCC 3 cut(s) 143, 455, 1888
BshTI ACCGGT 1 cut(s) 493
BshVI ATCGAT 1 cut(s) 201
BsiEI CGRYCG 1 cut(s) 105
BsiHKAI GWGCWC 3 cut(s) 332, 698, 992
BsiSI CCGG 6 cut(s) 78, 141, 273, 414, 494, 1181
BslFI GGGAC 1 cut(s) 786
BslI CCNNNNNNNGG 3 cut(s) 319, 1988, 2814
BsmAI GTCTC 6 cut(s) 297, 1101, 1128, 1254, 1258, 2754
BsmFI GGGAC 1 cut(s) 786
BsmI GAATGC 3 cut(s) 565, 610, 2588
BsnI GGCC 6 cut(s) 81, 104, 323, 2162, 2371, 2776
Bso31I GGTCTC 3 cut(s) 1101, 1128, 1254
Bsp1286I GDGCHC 3 cut(s) 332, 698, 992
Bsp19I CCATGG 1 cut(s) 1447
BspACI CCGC 9 cut(s) 105, 213, 313, 315, 445, 1346, 2149, 2474, 2716
BspANI GGCC 6 cut(s) 81, 104, 323, 2162, 2371, 2776
BspCNI CTCAG 5 cut(s) 1111, 1186, 1254, 1297, 1488
BspDI ATCGAT 1 cut(s) 201
BspFNI CGCG 5 cut(s) 164, 315, 445, 2646, 2648
BspHI TCATGA 1 cut(s) 1369
BspLI GGNNCC 8 cut(s) 145, 191, 278, 457, 1074, 1628, 1781, 1890
BspMAI CTGCAG 2 cut(s) 1023, 2173
BspMI ACCTGC 5 cut(s) 373, 439, 1083, 1209, 2729
BspPI GGATC 5 cut(s) 82, 1317, 1774, 1787, 2469
BspT107I GGYRCC 3 cut(s) 143, 455, 1888
BspTI CTTAAG 2 cut(s) 662, 1006
BspTNI GGTCTC 3 cut(s) 1101, 1128, 1254
BsrBI CCGCTC 1 cut(s) 2149
BsrFI RCCGGY 3 cut(s) 77, 140, 493
BsrI ACTGG 3 cut(s) 289, 1555, 1802
BssAI RCCGGY 3 cut(s) 77, 140, 493
BssECI CCNNGG 5 cut(s) 313, 1447, 2178, 2698, 2753
BssNI GRCGYC 2 cut(s) 138, 144
BssT1I CCWWGG 2 cut(s) 1447, 2753
Bst2UI CCWGG 7 cut(s) 194, 367, 1239, 1812, 2027, 2082, 2478
Bst4CI ACNGT 7 cut(s) 358, 463, 469, 922, 1567, 1600, 2617
Bst6I CTCTTC 1 cut(s) 2451
BstACI GRCGYC 2 cut(s) 138, 144
BstAFI CTTAAG 2 cut(s) 662, 1006
BstAPI GCANNNNNTGC 2 cut(s) 1895, 2663
BstBAI YACGTR 1 cut(s) 2663
BstC8I GCNNGC 9 cut(s) 79, 142, 1077, 1090, 1938, 2298, 2655, 2668, 2774
BstDEI CTNAG 9 cut(s) 1098, 1194, 1262, 1305, 1475, 1622, 1688, 2164, 2366
BstDSI CCRYGG 3 cut(s) 313, 1447, 2178
BstEII GGTNACC 1 cut(s) 2480
BstF5I GGATG 5 cut(s) 696, 895, 1150, 1754, 2431
BstFNI CGCG 5 cut(s) 164, 315, 445, 2646, 2648
BstH2I RGCGCY 2 cut(s) 147, 2329
BstHHI GCGC 6 cut(s) 77, 146, 166, 445, 2328, 2648
BstMAI GTCTC 6 cut(s) 297, 1101, 1128, 1254, 1258, 2754
BstMCI CGRYCG 1 cut(s) 105
BstNI CCWGG 7 cut(s) 194, 367, 1239, 1812, 2027, 2082, 2478
BstNSI RCATGY 3 cut(s) 2300, 2471, 2670
BstPI GGTNACC 1 cut(s) 2480
BstSCI CCNGG 8 cut(s) 192, 272, 365, 1237, 1810, 2025, 2080, 2476
BstSFI CTRYAG 3 cut(s) 1019, 1275, 2169
BstUI CGCG 5 cut(s) 164, 315, 445, 2646, 2648
BstV2I GAAGAC 2 cut(s) 443, 1628
BstX2I RGATCY 3 cut(s) 1779, 1930, 2461
BstXI CCANNNNNNTGG 1 cut(s) 2185
BstYI RGATCY 3 cut(s) 1779, 1930, 2461
BstZI CGGCCG 1 cut(s) 102
Bsu15I ATCGAT 1 cut(s) 201
Bsu36I CCTNAGG 2 cut(s) 1305, 1622
BsuRI GGCC 6 cut(s) 81, 104, 323, 2162, 2371, 2776
BsuTUI ATCGAT 1 cut(s) 201
BtgI CCRYGG 3 cut(s) 313, 1447, 2178
BtgZI GCGATG 2 cut(s) 1341, 2663
BtsCI GGATG 5 cut(s) 696, 895, 1150, 1754, 2431
BtsI GCAGTG 1 cut(s) 1875
BtsIMutI CAGTG 4 cut(s) 63, 918, 1875, 2613
BveI ACCTGC 5 cut(s) 373, 439, 1083, 1209, 2729
Cac8I GCNNGC 9 cut(s) 79, 142, 1077, 1090, 1938, 2298, 2655, 2668, 2774
CaiI CAGNNNCTG 3 cut(s) 72, 295, 1874
CciI TCATGA 1 cut(s) 1369
CfoI GCGC 6 cut(s) 77, 146, 166, 445, 2328, 2648
Cfr10I RCCGGY 3 cut(s) 77, 140, 493
Cfr13I GGNCC 6 cut(s) 61, 238, 369, 1444, 2029, 2160
Cfr42I CCGCGG 1 cut(s) 316
ClaI ATCGAT 1 cut(s) 201
CseI GACGC 1 cut(s) 146
Csp6I GTAC 4 cut(s) 376, 1286, 1427, 2314
CspAI ACCGGT 1 cut(s) 493
CspCI CAANNNNNGTGG 2 cut(s) 612, 647
CviQI GTAC 4 cut(s) 376, 1286, 1427, 2314
DdeI CTNAG 9 cut(s) 1098, 1194, 1262, 1305, 1475, 1622, 1688, 2164, 2366
DinI GGCGCC 1 cut(s) 145
DraI TTTAAA 1 cut(s) 2491
DriI GACNNNNNGTC 1 cut(s) 289
EaeI YGGCCR 2 cut(s) 79, 102
EagI CGGCCG 1 cut(s) 102
Eam1104I CTCTTC 1 cut(s) 2451
Eam1105I GACNNNNNGTC 1 cut(s) 289
EarI CTCTTC 1 cut(s) 2451
EciI GGCGGA 1 cut(s) 2463
Ecl136II GAGCTC 2 cut(s) 330, 990
EclXI CGGCCG 1 cut(s) 102
Eco130I CCWWGG 2 cut(s) 1447, 2753
Eco147I AGGCCT 1 cut(s) 323
Eco24I GRGCYC 2 cut(s) 332, 992
Eco31I GGTCTC 3 cut(s) 1101, 1128, 1254
Eco32I GATATC 1 cut(s) 2556
Eco47I GGWCC 5 cut(s) 61, 238, 369, 1444, 2029
Eco52I CGGCCG 1 cut(s) 102
Eco53kI GAGCTC 2 cut(s) 330, 990
Eco57I CTGAAG 8 cut(s) 1188, 1737, 1755, 1947, 2415, 2494, 2765, 2841
Eco72I CACGTG 1 cut(s) 2663
Eco81I CCTNAGG 2 cut(s) 1305, 1622
Eco91I GGTNACC 1 cut(s) 2480
EcoICRI GAGCTC 2 cut(s) 330, 990
EcoO109I RGGNCCY 3 cut(s) 238, 2029, 2160
EcoO65I GGTNACC 1 cut(s) 2480
EcoRII CCWGG 7 cut(s) 192, 365, 1237, 1810, 2025, 2080, 2476
EcoRV GATATC 1 cut(s) 2556
EcoT14I CCWWGG 2 cut(s) 1447, 2753
EcoT38I GRGCYC 2 cut(s) 332, 992
EgeI GGCGCC 1 cut(s) 145
EheI GGCGCC 1 cut(s) 145
ErhI CCWWGG 2 cut(s) 1447, 2753
FaqI GGGAC 1 cut(s) 786
FauNDI CATATG 1 cut(s) 1254
FblI GTMKAC 1 cut(s) 826
FokI GGATG 5 cut(s) 703, 882, 1157, 1741, 2438
FriOI GRGCYC 2 cut(s) 332, 992
FspBI CTAG 5 cut(s) 1038, 1140, 1455, 2061, 2637
GlaI GCGC 6 cut(s) 76, 145, 165, 444, 2327, 2647
GsaI CCCAGC 2 cut(s) 892, 1908
GsuI CTGGAG 4 cut(s) 272, 1302, 1833, 2103
HaeII RGCGCY 2 cut(s) 147, 2329
HaeIII GGCC 6 cut(s) 81, 104, 323, 2162, 2371, 2776
HapII CCGG 6 cut(s) 78, 141, 273, 414, 494, 1181
HgaI GACGC 1 cut(s) 146
HhaI GCGC 6 cut(s) 77, 146, 166, 445, 2328, 2648
Hin1I GRCGYC 2 cut(s) 138, 144
Hin6I GCGC 6 cut(s) 75, 144, 164, 443, 2326, 2646
HinP1I GCGC 6 cut(s) 75, 144, 164, 443, 2326, 2646
HincII GTYRAC 2 cut(s) 827, 2620
HindII GTYRAC 2 cut(s) 827, 2620
HindIII AAGCTT 1 cut(s) 854
HpaII CCGG 6 cut(s) 78, 141, 273, 414, 494, 1181
HphI GGTGA 8 cut(s) 508, 1101, 1754, 1841, 1893, 1938, 2239, 2474
Hpy166II GTNNAC 7 cut(s) 64, 659, 827, 1109, 1429, 1850, 2620
Hpy8I GTNNAC 7 cut(s) 64, 659, 827, 1109, 1429, 1850, 2620
Hpy99I CGWCG 2 cut(s) 140, 304
HpyCH4III ACNGT 7 cut(s) 358, 463, 469, 922, 1567, 1600, 2617
HpyCH4IV ACGT 2 cut(s) 302, 2662
HpyF3I CTNAG 9 cut(s) 1098, 1194, 1262, 1305, 1475, 1622, 1688, 2164, 2366
HpySE526I ACGT 2 cut(s) 302, 2662
Hsp92I GRCGYC 2 cut(s) 138, 144
HspAI GCGC 6 cut(s) 75, 144, 164, 443, 2326, 2646
KasI GGCGCC 1 cut(s) 143
KroI GCCGGC 2 cut(s) 77, 140
KroNI GCCGGC 2 cut(s) 79, 142
KspI CCGCGG 1 cut(s) 316
LmnI GCTCC 6 cut(s) 995, 1072, 1336, 2122, 2146, 2476
MaeI CTAG 5 cut(s) 1038, 1140, 1455, 2061, 2637
MaeII ACGT 2 cut(s) 302, 2662
MbiI CCGCTC 1 cut(s) 2149
MfeI CAATTG 1 cut(s) 1460
MflI RGATCY 3 cut(s) 1779, 1930, 2461
MhlI GDGCHC 3 cut(s) 332, 698, 992
Mly113I GGCGCC 1 cut(s) 144
MlyI GAGTC 1 cut(s) 2615
MmeI TCCRAC 4 cut(s) 767, 950, 2166, 2361
MreI CGCCGGCG 1 cut(s) 140
MroNI GCCGGC 2 cut(s) 77, 140
MroXI GAANNNNTTC 3 cut(s) 655, 971, 1173
MslI CAYNNNNRTG 4 cut(s) 414, 2098, 2424, 2665
MspA1I CMGCKG 3 cut(s) 315, 1940, 2303
MspCI CTTAAG 2 cut(s) 662, 1006
MspI CCGG 6 cut(s) 78, 141, 273, 414, 494, 1181
MspR9I CCNGG 8 cut(s) 194, 274, 367, 1239, 1812, 2027, 2082, 2478
MunI CAATTG 1 cut(s) 1460
Mva1269I GAATGC 3 cut(s) 565, 610, 2588
MvaI CCWGG 7 cut(s) 194, 367, 1239, 1812, 2027, 2082, 2478
MvnI CGCG 5 cut(s) 164, 315, 445, 2646, 2648
NaeI GCCGGC 2 cut(s) 79, 142
NarI GGCGCC 1 cut(s) 144
NciI CCSGG 1 cut(s) 274
NcoI CCATGG 1 cut(s) 1447
NdeI CATATG 1 cut(s) 1254
NgoMIV GCCGGC 2 cut(s) 77, 140
NlaIV GGNNCC 8 cut(s) 145, 191, 278, 457, 1074, 1628, 1781, 1890
NmeAIII GCCGAG 1 cut(s) 80
NmuCI GTSAC 3 cut(s) 676, 2331, 2480
NspI RCATGY 3 cut(s) 2300, 2471, 2670
OliI CACNNNNGTG 1 cut(s) 2098
PaeI GCATGC 2 cut(s) 2300, 2670
PagI TCATGA 1 cut(s) 1369
PceI AGGCCT 1 cut(s) 323
PcsI WCGNNNNNNNCGW 1 cut(s) 459
PctI GAATGC 3 cut(s) 565, 610, 2588
PdiI GCCGGC 2 cut(s) 79, 142
PdmI GAANNNNTTC 3 cut(s) 655, 971, 1173
PfoI TCCNGGA 2 cut(s) 1810, 2025
PinAI ACCGGT 1 cut(s) 493
PleI GAGTC 1 cut(s) 2615
PluTI GGCGCC 1 cut(s) 147
PmaCI CACGTG 1 cut(s) 2663
PmlI CACGTG 1 cut(s) 2663
PpsI GAGTC 1 cut(s) 2615
Ppu21I YACGTR 1 cut(s) 2663
PpuMI RGGWCCY 2 cut(s) 238, 2029
Psp124BI GAGCTC 2 cut(s) 332, 992
Psp5II RGGWCCY 2 cut(s) 238, 2029
Psp6I CCWGG 7 cut(s) 192, 365, 1237, 1810, 2025, 2080, 2476
PspCI CACGTG 1 cut(s) 2663
PspEI GGTNACC 1 cut(s) 2480
PspFI CCCAGC 2 cut(s) 888, 1904
PspGI CCWGG 7 cut(s) 192, 365, 1237, 1810, 2025, 2080, 2476
PspN4I GGNNCC 8 cut(s) 145, 191, 278, 457, 1074, 1628, 1781, 1890
PspPI GGNCC 6 cut(s) 61, 238, 369, 1444, 2029, 2160
PspPPI RGGWCCY 2 cut(s) 238, 2029
PstI CTGCAG 2 cut(s) 1023, 2173
PstNI CAGNNNCTG 3 cut(s) 72, 295, 1874
PsuI RGATCY 3 cut(s) 1779, 1930, 2461
PvuII CAGCTG 2 cut(s) 1940, 2303
RsaI GTAC 4 cut(s) 377, 1287, 1428, 2315
RsaNI GTAC 4 cut(s) 376, 1286, 1427, 2314
RseI CAYNNNNRTG 4 cut(s) 414, 2098, 2424, 2665
SacI GAGCTC 2 cut(s) 332, 992
SacII CCGCGG 1 cut(s) 316
SalI GTCGAC 1 cut(s) 825
Sau96I GGNCC 6 cut(s) 61, 238, 369, 1444, 2029, 2160
SchI GAGTC 1 cut(s) 2615
ScrFI CCNGG 8 cut(s) 194, 274, 367, 1239, 1812, 2027, 2082, 2478
SduI GDGCHC 3 cut(s) 332, 698, 992
SfcI CTRYAG 3 cut(s) 1019, 1275, 2169
SfoI GGCGCC 1 cut(s) 145
Sfr303I CCGCGG 1 cut(s) 316
SgrAI CRCCGGYG 1 cut(s) 140
SgrBI CCGCGG 1 cut(s) 316
SinI GGWCC 5 cut(s) 61, 238, 369, 1444, 2029
SmiMI CAYNNNNRTG 4 cut(s) 414, 2098, 2424, 2665
SmlI CTYRAG 6 cut(s) 248, 325, 662, 1006, 2011, 2726
SmoI CTYRAG 6 cut(s) 248, 325, 662, 1006, 2011, 2726
SpeI ACTAGT 2 cut(s) 1037, 1454
SphI GCATGC 2 cut(s) 2300, 2670
SseBI AGGCCT 1 cut(s) 323
SsiI CCGC 9 cut(s) 105, 213, 313, 315, 445, 1346, 2149, 2474, 2716
SspDI GGCGCC 1 cut(s) 143
SspI AATATT 2 cut(s) 673, 1763
SspMI CTAG 5 cut(s) 1038, 1140, 1455, 2061, 2637
SstI GAGCTC 2 cut(s) 332, 992
StuI AGGCCT 1 cut(s) 323
StyD4I CCNGG 8 cut(s) 192, 272, 365, 1237, 1810, 2025, 2080, 2476
StyI CCWWGG 2 cut(s) 1447, 2753
TaaI ACNGT 7 cut(s) 358, 463, 469, 922, 1567, 1600, 2617
TaiI ACGT 2 cut(s) 305, 2665
TaqI TCGA 9 cut(s) 93, 123, 135, 156, 201, 826, 1495, 1967, 2006
TatI WGTACW 2 cut(s) 1426, 2313
TauI GCSGC 4 cut(s) 107, 216, 318, 448
TscAI CASTG 4 cut(s) 63, 925, 1875, 2620
TseFI GTSAC 3 cut(s) 676, 2331, 2480
Tsp45I GTSAC 3 cut(s) 676, 2331, 2480
TspDTI ATGAA 6 cut(s) 1407, 1499, 1506, 1628, 2237, 2242
TspGWI ACGGA 4 cut(s) 9, 443, 2195, 2614
TspRI CASTG 4 cut(s) 63, 925, 1875, 2620
Vha464I CTTAAG 2 cut(s) 662, 1006
VpaK11BI GGWCC 5 cut(s) 61, 238, 369, 1444, 2029
XapI RAATTY 4 cut(s) 349, 487, 913, 1396
XbaI TCTAGA 2 cut(s) 2060, 2636
XceI RCATGY 3 cut(s) 2300, 2471, 2670
XmiI GTMKAC 1 cut(s) 826
XmnI GAANNNNTTC 3 cut(s) 655, 971, 1173
XspI CTAG 5 cut(s) 1038, 1140, 1455, 2061, 2637
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.