RLG00000010787
NAC Family

Probably inactive leucine-rich repeat receptor-like protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0008421
Physical Location & Seq
Reverse (-)
4345483 .. 4349755
4273 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000010787

Sequence Viewer

Length: 3255 bp
ATGAGCTTCAGTGCTTTCTACGTGGACGTTATAACATTTGAGCTGAAGATGGCGAAGAGACCTCGAAGCACTAGTCTCATATCTTGCATGTTGATCATCATGTTCTTAATCTTCCCAAATCATATCTGTGCTTTGGATGGTTATGATGACGCTGAAGTTCAGCTTCTCTTGTCCTTCAAAGCTTCCATCTTCGACGACCCTTCACACTCCCTCTCAAGCTGGAGTAACTCCACTGCCAACAATCTCTGCAACTGGCACGGCGTTACATGCATCGGTGATGTTAACTCGTCGCACGTCAACGTCATCGACCTCTCTGGAAGAAACATCTCTGGCAGATTATCATCCTCACTCTTTCACTTGTCCCACGTCGAAAAAATCGACCTCTCCAACAATCAGCTACATGGCCAACTTCCCCGTGACATATTCACCAGCTCAAGTTCTCTCCGACATCTCAATCTCAGCAACAACAACTTGACCGGCCAAATCCCACATGGCTCACTTCCGGGTCTGGAGACACTAGACTTATGTAACAACATGATTTCGGGGAAAATCCCCGAAAACATTGGTTTGTTCTCGAGATTAAAGTTTCTTGATCTTGGTGGTAATGTGTTAGCGGGAGAAATTCCAAATTCCATGTCAAATATATCTAACTTGCAGTACTTGACTTTGGCTTCAAACCAACTAATTGGCAAAGTTCCAAGCCAATTAGGCCAAGTCAAGAGCTTAAAGTTGATATACTTGGGCTACAACAATCTCTCCGGCGAAATCCCACAAGAGATTGGCAAGCTTACTGCTTTGAATCATCTCGATCTCGTTTTCAACAATCTCACCGGCCAAATCCCGGACTCTCTAGGAAACCTTACCGAGCTCCGATATCTCTTCCTCTACGGAAACAAGCTCACAGGTCCGCTTCCTAAATCCATTTTTGGTCTTCAAAAGCTTGTCTCACTTGATCTTAGTGAGAATTCTCTTTCGGGTGAGATTCCTGAACTTGTTAGTCAGCTACAACAACTTGAGATTCTTCATCTTTTCGCAAACAACTTTACCGGAAAGATTCCGAAATCCCTTGCTTCTCTGTCTCGGCTGCAAGTCCTTCAGCTGTGGTCGAACAAGTTCTCAGGGGAGATTCCGAGTGATCTTGGAAAGCAAAGCAATCTCACTGTTGTAGACTTATCTACAAACTACCTCACCGGAAAAGTACCTAATACGATATGCCACTCGGGGAACCTTTTCAAGCTCATCCTCTTTTCCAATTCTCTTGAAGGTGAAATCACAAAAAGTCTGGCTTCTTGCAAGAGCTTAGGCCGAGTTCGAGTTCAAAACAACCGGCTCTCCGGTGAAATATCGGCCGAATTTGTAAAGCTTCCGCTTGTGTATTTCTTGGATATCTCAGGCAATAATTTTTCCGGAAGAATTGATGACAAAAAGTGGGGCCTGCCTTCACTTCAAATGCTGAATATCGCGAGAAACAGACTTTTCGGCAACTTACCGGAGTCTTTCGGCAGCGACGAGCTGGAGAACTTAGACTTGTCAGAAAATCTTTTATCGGGAAATATCTCCCTGAATTTCGGGAACTTATCGGAGCTGATGCAGTTAAAGCTTTCTCACAATAAGCTCTCTGGTCCCATCCCTCAACAATTGTCTTCATGTAAAAAGCTCGTGGGCCTAGACCTCAGCCGCAACCAGCTCAGCGGCCCAATTCCGGTCAGCCTTTCTGAGATGCCAGTTCTCGGTCAGCTTGATTTGTCCTACAACCAACTTTCCGGCGAAATTCCACGGAACCTAGGCGTGATTGAATCGCTTGTTCAAGTGAATATTTCTCACAACCACCTTCACGGAAACTTGCCTTCCACCGGAGCCTTTCTTGCTATAAATGCGAGCTCGGTGGCAGGAAATGACCTTTGTGGCGGCGACACCACGAGTGGTTTGCCACCATGCAAGAGCAAAAGCGTAAGAAACAACCCCACGTGGTGGTTTATTCTCACTTGCTTTCTCGTTGCTTTAGCTGCTTTTGGTGTTGCTGGTTTTGTCATTGTATACATTCGGAGACGAAAGGATTTGGAGCTAAAAACCGTGGAGAATGAAGATGGAATTTGGAAAATGCAATTCTTTGAGCCCAAAGTTTCAAGATCGGTTTCAATTGAAGATATTTTATCAGCAGCAAAACAAGGTAATATCATTGCAATTGGGAACAAAGGTGCACAATTTGTTGTAAAAGATGATGCTGTGAATTCAATTTCACCAACATTTTGGTCTAAAATGGTGGAATTTGGAAACCTTCAGCACCCCAACATTATCCGGTTGATCGGAATATGTCGTTCAGAAAAGAATGCATATGTCATCCACGAATATTGCGAAGGGAAAGCATTGTGTCAAATTCTGCGGAAGATAAACTGGGAACAACGACGGAAAATTGCCGTTGGGATTGCAAGAGCTCTACGGTTCTTGCATGTTTCTTGCTCTCCGAGTGTTGTCATCGGTTGTGTGTCTCCCGAGAAAGTAATTGTTGACGCAAAAAATGAGCCTCGTCTTCAGTTGAGTCTCCCTGAGTTTAGGGACTCCAAGGGTTTTGCTTCTTCTGCATACGTTTCTCCAGAAGCTAAAAACAGCAAAGACATCACTGAGAAAAGTGATATCTATGGTTTTGGTCTAGTCCTCATTGAATTACTGACCGGGAAAAGCCCCGACGACGTAGAGTTGGGCGCACACGAGAGCATCGTTGAGTGGGCTCGGTATTGCTACTCGGATTGCCATCTTGATGCGTGGACCGACCCGATGATCCGAGGACACGTGTTCAAGAATCAGAATGAAATTGTTGAAACCATGAACCTAGCCCTTCACTGTACTGCTGGTGACCCCACTGCTAGACCATGCGCAAATGAACTATACAAAACCCTAGACTCTATAACGAAGACAAGTTCTTGTGTTGCATTGGAGGGCCTCATCGGTGGAGGCGGCGTCGTCCGGCTGGACCAAAACGCCGTCATCGAAACGGATGGGCTTGACGTCTCTGATCTCTGTGACGACTGTGAAGAATCCCGAGGAGGAGGAAGAGGAGGGGAGGCGCCTGAGGGAGATGGAGTAGTAGGAGATGAAGAGGGTCTTGCAAGGCCTCACGGTGGCCGAGACTGCCAAGATCGAGCGGACCAGCAGAGCTATCGGGAGGGGTCTTATAACCATAGGCAAGTTTCCATGGAAACTGCAACGCAGCAAGTCAATTTGGGGTTGAGACTACATAGGAGACATAGTACGTGA

Protein Analysis

1085

Amino Acids

118.74

Weight (kDa)

6.06

Isoelectric Point (pI)

39.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 50 - 90 1.7e-11 Leucine rich repeat N-terminal domain
LRR_14 PF23598 115 - 242 1.9e-06 Leucine-rich repeat region
LRR_4 PF12799 147 - 180 2.1e-06 Leucine Rich repeats (2 copies)
LRR_8 PF13855 216 - 276 7.3e-07 Leucine rich repeat
LRR_8 PF13855 265 - 324 2.8e-07 Leucine rich repeat
LRR_14 PF23598 282 - 407 1.2e-14 Leucine-rich repeat region
LRR_8 PF13855 289 - 348 4.1e-06 Leucine rich repeat
LRR_8 PF13855 504 - 563 2.8e-06 Leucine rich repeat
PK_Tyr_Ser-Thr PF07714 745 - 966 5.3e-17 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 757 - 963 1.4e-17 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 32, 3174
AatII GACGTC 1 cut(s) 3009
Acc16I TGCGCA 1 cut(s) 2875
AccB1I GGYRCC 1 cut(s) 3064
AccB7I CCANNNNNTGG 1 cut(s) 1971
AccBSI CCGCTC 1 cut(s) 3143
AccI GTMKAC 2 cut(s) 1167, 2037
AccII CGCG 1 cut(s) 1463
AccIII TCCGGA 1 cut(s) 1406
AciI CCGC 9 cut(s) 614, 908, 1367, 1678, 1692, 1908, 2383, 2955, 3143
AclWI GGATC 1 cut(s) 2773
AcoI YGGCCR 5 cut(s) 403, 478, 832, 1347, 3121
AcuI CTGAAG 5 cut(s) 65, 174, 1079, 2264, 2516
AcvI CACGTG 2 cut(s) 1968, 2791
AcyI GRCGYC 3 cut(s) 2958, 3006, 3065
AdeI CACNNNGTG 1 cut(s) 1971
AfaI GTAC 4 cut(s) 659, 1200, 2845, 3250
AfiI CCNNNNNNNGG 6 cut(s) 841, 1702, 1730, 1853, 1971, 3119
AflIII ACRYGT 2 cut(s) 2788, 2790
AhlI ACTAGT 1 cut(s) 71
AjiI CACGTC 2 cut(s) 295, 367
Alw21I GWGCWC 4 cut(s) 870, 1883, 2203, 2437
Alw44I GTGCAC 1 cut(s) 2199
AlwI GGATC 1 cut(s) 2773
Ama87I CYCGRG 4 cut(s) 574, 1219, 2492, 3039
Aor13HI TCCGGA 1 cut(s) 1406
ApaLI GTGCAC 1 cut(s) 2199
ApeKI GCWGC 5 cut(s) 1084, 1503, 2006, 2159, 3208
ArsI GACNNNNNNTTYG 4 cut(s) 58, 90, 620, 652
Asp700I GAANNNNTTC 2 cut(s) 1112, 1229
AspA2I CCTAGG 1 cut(s) 1783
AspLEI GCGC 3 cut(s) 2705, 2876, 3067
AspS9I GGNCC 9 cut(s) 905, 1432, 1622, 1663, 1694, 2766, 2938, 2971, 3145
AsuC2I CCSGG 3 cut(s) 504, 842, 2674
AsuHPI GGTGA 9 cut(s) 287, 418, 820, 989, 1180, 1277, 1349, 2232, 2864
AvaI CYCGRG 4 cut(s) 574, 1219, 2492, 3039
AvaII GGWCC 5 cut(s) 905, 1622, 2766, 2971, 3145
AvrII CCTAGG 1 cut(s) 1783
AxyI CCTNAGG 1 cut(s) 3069
BaeGI GKGCMC 1 cut(s) 2203
BalI TGGCCA 1 cut(s) 405
BanI GGYRCC 1 cut(s) 3064
BanII GRGCYC 5 cut(s) 870, 1883, 2118, 2437, 2731
BauI CACGAG 3 cut(s) 1658, 1918, 2708
BbrPI CACGTG 2 cut(s) 1968, 2791
BbsI GAAGAC 4 cut(s) 923, 1635, 2522, 2918
Bbv12I GWGCWC 4 cut(s) 870, 1883, 2203, 2437
BbvCI CCTCAGC 1 cut(s) 1673
BbvI GCAGC 5 cut(s) 1071, 1515, 1993, 2171, 3220
BccI CCATC 8 cut(s) 43, 131, 194, 1634, 2081, 2760, 2990, 3071
BceAI ACGGC 3 cut(s) 274, 2402, 2966
BcgI CGANNNNNNTGC 4 cut(s) 1909, 1943, 3140, 3174
BclI TGATCA 1 cut(s) 93
BcnI CCSGG 3 cut(s) 504, 842, 2674
BcuI ACTAGT 1 cut(s) 71
BfaI CTAG 9 cut(s) 72, 518, 851, 1667, 1784, 2651, 2831, 2865, 2897
BfoI RGCGCY 1 cut(s) 3068
BglI GCCNNNNNGGC 1 cut(s) 708
BisI GCNGC 9 cut(s) 1085, 1504, 1678, 1693, 1909, 2007, 2160, 2956, 3209
BlnI CCTAGG 1 cut(s) 1783
BlpI GCTNAGC 1 cut(s) 1688
BlsI GCNGC 9 cut(s) 1086, 1505, 1679, 1694, 1910, 2008, 2161, 2957, 3210
BmcAI AGTACT 1 cut(s) 659
Bme1390I CCNGG 3 cut(s) 504, 842, 2674
Bme18I GGWCC 5 cut(s) 905, 1622, 2766, 2971, 3145
BmeT110I CYCGRG 4 cut(s) 574, 1219, 2492, 3039
BmgBI CACGTC 2 cut(s) 295, 367
BmgT120I GGNCC 9 cut(s) 905, 1432, 1622, 1663, 1694, 2766, 2938, 2971, 3145
BmiI GGNNCC 6 cut(s) 1226, 1433, 1624, 1781, 1858, 3066
BmrFI CCNGG 3 cut(s) 504, 842, 2674
BmrI ACTGGG 1 cut(s) 2404
BmsI GCATC 6 cut(s) 279, 1578, 1710, 2212, 2724, 2749
BmuI ACTGGG 1 cut(s) 2404
BpiI GAAGAC 4 cut(s) 923, 1635, 2522, 2918
BpmI CTGGAG 4 cut(s) 241, 530, 1535, 2577
Bpu10I CCTNAGC 2 cut(s) 1300, 1673
Bpu1102I GCTNAGC 1 cut(s) 1688
BpuEI CTTGAG 3 cut(s) 199, 418, 1034
BpuMI CCSGG 3 cut(s) 504, 842, 2674
BsaAI YACGTR 4 cut(s) 22, 1968, 2791, 3252
BsaBI GATNNNNATC 2 cut(s) 340, 2751
BsaHI GRCGYC 3 cut(s) 2958, 3006, 3065
BsaI GGTCTC 1 cut(s) 52
BsaJI CCNNGG 7 cut(s) 1775, 1783, 2073, 2562, 2782, 3040, 3192
BsaWI WCCGGW 8 cut(s) 1046, 1190, 1334, 1406, 1489, 1702, 1853, 2298
BsaXI ACNNNNNCTCC 9 cut(s) 740, 770, 1316, 1346, 2943, 2973, 3066, 3096, 3232
Bsc4I CCNNNNNNNGG 6 cut(s) 841, 1702, 1730, 1853, 1971, 3119
Bse118I RCCGGY 3 cut(s) 476, 830, 1326
Bse1I ACTGG 3 cut(s) 257, 1724, 2399
Bse21I CCTNAGG 1 cut(s) 3069
Bse3DI GCAATG 1 cut(s) 2178
Bse8I GATNNNNATC 2 cut(s) 340, 2751
BseAI TCCGGA 1 cut(s) 1406
BseDI CCNNGG 7 cut(s) 1775, 1783, 2073, 2562, 2782, 3040, 3192
BseGI GGATG 6 cut(s) 142, 341, 1239, 1626, 2340, 3001
BseJI GATNNNNATC 2 cut(s) 340, 2751
BseLI CCNNNNNNNGG 6 cut(s) 841, 1702, 1730, 1853, 1971, 3119
BseMI GCAATG 1 cut(s) 2178
BseMII CTCAG 9 cut(s) 472, 1131, 1404, 1687, 1702, 1707, 2538, 2613, 3060
BseNI ACTGG 3 cut(s) 257, 1724, 2399
BseRI GAGGAG 3 cut(s) 3057, 3060, 3069
BseSI GKGCMC 1 cut(s) 2203
BseX3I CGGCCG 1 cut(s) 1347
BseXI GCAGC 5 cut(s) 1071, 1515, 1993, 2171, 3220
Bsh1236I CGCG 1 cut(s) 1463
Bsh1285I CGRYCG 1 cut(s) 1350
BshNI GGYRCC 1 cut(s) 3064
BsiEI CGRYCG 1 cut(s) 1350
BsiHKAI GWGCWC 4 cut(s) 870, 1883, 2203, 2437
BsiHKCI CYCGRG 4 cut(s) 574, 1219, 2492, 3039
BslFI GGGAC 3 cut(s) 346, 1608, 2570
BslI CCNNNNNNNGG 6 cut(s) 841, 1702, 1730, 1853, 1971, 3119
BsmBI CGTCTC 2 cut(s) 2041, 3013
BsmFI GGGAC 3 cut(s) 346, 1608, 2570
BsmI GAATGC 1 cut(s) 2335
Bso31I GGTCTC 1 cut(s) 52
BsoBI CYCGRG 4 cut(s) 574, 1219, 2492, 3039
Bsp1286I GDGCHC 6 cut(s) 870, 1883, 2118, 2203, 2437, 2731
Bsp13I TCCGGA 1 cut(s) 1406
Bsp1720I GCTNAGC 1 cut(s) 1688
Bsp19I CCATGG 1 cut(s) 3192
Bsp68I TCGCGA 1 cut(s) 1463
BspACI CCGC 9 cut(s) 614, 908, 1367, 1678, 1692, 1908, 2383, 2955, 3143
BspCNI CTCAG 9 cut(s) 471, 1130, 1403, 1686, 1701, 1708, 2539, 2614, 3061
BspEI TCCGGA 1 cut(s) 1406
BspFNI CGCG 1 cut(s) 1463
BspLI GGNNCC 6 cut(s) 1226, 1433, 1624, 1781, 1858, 3066
BspPI GGATC 1 cut(s) 2773
BspT107I GGYRCC 1 cut(s) 3064
BspTNI GGTCTC 1 cut(s) 52
BsrBI CCGCTC 1 cut(s) 3143
BsrDI GCAATG 1 cut(s) 2178
BsrFI RCCGGY 3 cut(s) 476, 830, 1326
BsrI ACTGG 3 cut(s) 257, 1724, 2399
BssAI RCCGGY 3 cut(s) 476, 830, 1326
BssECI CCNNGG 7 cut(s) 1775, 1783, 2073, 2562, 2782, 3040, 3192
BssNAI GTATAC 1 cut(s) 2038
BssNI GRCGYC 3 cut(s) 2958, 3006, 3065
BssSI CACGAG 3 cut(s) 1658, 1918, 2708
BssT1I CCWWGG 3 cut(s) 1783, 2562, 3192
Bst1107I GTATAC 1 cut(s) 2038
Bst2BI CACGAG 3 cut(s) 1658, 1918, 2708
Bst4CI ACNGT 6 cut(s) 1162, 2074, 2442, 2843, 3029, 3119
Bst6I CTCTTC 4 cut(s) 50, 884, 3046, 3090
BstACI GRCGYC 3 cut(s) 2958, 3006, 3065
BstBAI YACGTR 4 cut(s) 22, 1968, 2791, 3252
BstC8I GCNNGC 3 cut(s) 785, 1436, 1879
BstDSI CCRYGG 3 cut(s) 1775, 2073, 3192
BstEII GGTNACC 1 cut(s) 2852
BstF5I GGATG 6 cut(s) 142, 341, 1239, 1626, 2340, 3001
BstFNI CGCG 1 cut(s) 1463
BstH2I RGCGCY 1 cut(s) 3068
BstHHI GCGC 3 cut(s) 2705, 2876, 3067
BstMCI CGRYCG 1 cut(s) 1350
BstMWI GCNNNNNNNGC 8 cut(s) 267, 708, 1597, 1865, 1874, 2006, 2579, 3129
BstNSI RCATGY 3 cut(s) 91, 270, 2453
BstPI GGTNACC 1 cut(s) 2852
BstSCI CCNGG 3 cut(s) 502, 840, 2672
BstSLI GKGCMC 1 cut(s) 2203
BstUI CGCG 1 cut(s) 1463
BstV1I GCAGC 5 cut(s) 1071, 1515, 1993, 2171, 3220
BstV2I GAAGAC 4 cut(s) 923, 1635, 2522, 2918
BstXI CCANNNNNNTGG 2 cut(s) 686, 2250
BstZ17I GTATAC 1 cut(s) 2038
BstZI CGGCCG 1 cut(s) 1347
Bsu36I CCTNAGG 1 cut(s) 3069
BtgI CCRYGG 3 cut(s) 1775, 2073, 3192
BtrI CACGTC 2 cut(s) 295, 367
BtsCI GGATG 6 cut(s) 142, 341, 1239, 1626, 2340, 3001
BtsI GCAGTG 2 cut(s) 231, 2859
BtsIMutI CAGTG 6 cut(s) 16, 231, 1158, 2619, 2839, 2859
BtuMI TCGCGA 1 cut(s) 1463
Cac8I GCNNGC 3 cut(s) 785, 1436, 1879
CfoI GCGC 3 cut(s) 2705, 2876, 3067
Cfr10I RCCGGY 3 cut(s) 476, 830, 1326
Cfr13I GGNCC 9 cut(s) 905, 1432, 1622, 1663, 1694, 2766, 2938, 2971, 3145
CseI GACGC 3 cut(s) 158, 2519, 2947
Csp6I GTAC 4 cut(s) 658, 1199, 2844, 3249
CspCI CAANNNNNGTGG 2 cut(s) 2333, 2368
CviQI GTAC 4 cut(s) 658, 1199, 2844, 3249
DinI GGCGCC 1 cut(s) 3066
DraIII CACNNNGTG 1 cut(s) 1971
EaeI YGGCCR 5 cut(s) 403, 478, 832, 1347, 3121
EagI CGGCCG 1 cut(s) 1347
Eam1104I CTCTTC 4 cut(s) 50, 884, 3046, 3090
EarI CTCTTC 4 cut(s) 50, 884, 3046, 3090
Ecl136II GAGCTC 3 cut(s) 868, 1881, 2435
EclXI CGGCCG 1 cut(s) 1347
Eco130I CCWWGG 3 cut(s) 1783, 2562, 3192
Eco147I AGGCCT 1 cut(s) 3112
Eco24I GRGCYC 5 cut(s) 870, 1883, 2118, 2437, 2731
Eco31I GGTCTC 1 cut(s) 52
Eco32I GATATC 3 cut(s) 875, 1387, 2635
Eco47I GGWCC 5 cut(s) 905, 1622, 2766, 2971, 3145
Eco52I CGGCCG 1 cut(s) 1347
Eco53kI GAGCTC 3 cut(s) 868, 1881, 2435
Eco57I CTGAAG 5 cut(s) 65, 174, 1079, 2264, 2516
Eco72I CACGTG 2 cut(s) 1968, 2791
Eco81I CCTNAGG 1 cut(s) 3069
Eco88I CYCGRG 4 cut(s) 574, 1219, 2492, 3039
Eco91I GGTNACC 1 cut(s) 2852
EcoICRI GAGCTC 3 cut(s) 868, 1881, 2435
EcoO109I RGGNCCY 2 cut(s) 1432, 2938
EcoO65I GGTNACC 1 cut(s) 2852
EcoRI GAATTC 2 cut(s) 964, 2230
EcoRV GATATC 3 cut(s) 875, 1387, 2635
EcoT14I CCWWGG 3 cut(s) 1783, 2562, 3192
EcoT22I ATGCAT 2 cut(s) 272, 2335
EcoT38I GRGCYC 5 cut(s) 870, 1883, 2118, 2437, 2731
EgeI GGCGCC 1 cut(s) 3066
EheI GGCGCC 1 cut(s) 3066
ErhI CCWWGG 3 cut(s) 1783, 2562, 3192
Esp3I CGTCTC 2 cut(s) 2041, 3013
FalI AAGNNNNNCTT 6 cut(s) 147, 179, 1270, 1302, 3087, 3119
FaqI GGGAC 3 cut(s) 346, 1608, 2570
FauI CCCGC 1 cut(s) 607
FauNDI CATATG 1 cut(s) 2335
FbaI TGATCA 1 cut(s) 93
FblI GTMKAC 2 cut(s) 1167, 2037
Fnu4HI GCNGC 9 cut(s) 1085, 1504, 1678, 1693, 1909, 2007, 2160, 2956, 3209
FokI GGATG 6 cut(s) 149, 328, 1226, 1613, 2327, 3008
FriOI GRGCYC 5 cut(s) 870, 1883, 2118, 2437, 2731
Fsp4HI GCNGC 9 cut(s) 1085, 1504, 1678, 1693, 1909, 2007, 2160, 2956, 3209
FspBI CTAG 9 cut(s) 72, 518, 851, 1667, 1784, 2651, 2831, 2865, 2897
FspI TGCGCA 1 cut(s) 2875
GlaI GCGC 3 cut(s) 2704, 2875, 3066
GluI GCNGC 9 cut(s) 1085, 1504, 1678, 1693, 1909, 2007, 2160, 2956, 3209
GsuI CTGGAG 4 cut(s) 241, 530, 1535, 2577
HaeII RGCGCY 1 cut(s) 3068
HgaI GACGC 3 cut(s) 158, 2519, 2947
HhaI GCGC 3 cut(s) 2705, 2876, 3067
Hin1I GRCGYC 3 cut(s) 2958, 3006, 3065
Hin6I GCGC 3 cut(s) 2703, 2874, 3065
HinP1I GCGC 3 cut(s) 2703, 2874, 3065
HincII GTYRAC 3 cut(s) 283, 298, 2509
HindII GTYRAC 3 cut(s) 283, 298, 2509
HindIII AAGCTT 5 cut(s) 180, 785, 938, 1361, 1598
HpaI GTTAAC 1 cut(s) 283
HphI GGTGA 9 cut(s) 287, 418, 820, 989, 1180, 1277, 1349, 2232, 2864
Hpy166II GTNNAC 8 cut(s) 25, 283, 298, 1168, 2038, 2201, 2509, 2766
Hpy8I GTNNAC 8 cut(s) 25, 283, 298, 1168, 2038, 2201, 2509, 2766
Hpy99I CGWCG 8 cut(s) 197, 292, 371, 1511, 2409, 2690, 2693, 2963
HpyCH4III ACNGT 6 cut(s) 1162, 2074, 2442, 2843, 3029, 3119
HpyF10VI GCNNNNNNNGC 8 cut(s) 267, 708, 1597, 1865, 1874, 2006, 2579, 3129
Hsp92I GRCGYC 3 cut(s) 2958, 3006, 3065
HspAI GCGC 3 cut(s) 2703, 2874, 3065
KasI GGCGCC 1 cut(s) 3064
Kpn2I TCCGGA 1 cut(s) 1406
Ksp22I TGATCA 1 cut(s) 93
KspAI GTTAAC 1 cut(s) 283
LmnI GCTCC 4 cut(s) 873, 1582, 1856, 2062
Lsp1109I GCAGC 5 cut(s) 1071, 1515, 1993, 2171, 3220
LweI GCATC 6 cut(s) 279, 1578, 1710, 2212, 2724, 2749
MaeI CTAG 9 cut(s) 72, 518, 851, 1667, 1784, 2651, 2831, 2865, 2897
MaeIII GTNAC 6 cut(s) 224, 262, 416, 527, 2852, 3020
MbiI CCGCTC 1 cut(s) 3143
MfeI CAATTG 3 cut(s) 1637, 2139, 2184
MhlI GDGCHC 6 cut(s) 870, 1883, 2118, 2203, 2437, 2731
MlsI TGGCCA 1 cut(s) 405
MluNI TGGCCA 1 cut(s) 405
Mly113I GGCGCC 1 cut(s) 3065
MlyI GAGTC 5 cut(s) 839, 1502, 2548, 2552, 2894
MmeI TCCRAC 2 cut(s) 411, 469
Mox20I TGGCCA 1 cut(s) 405
Mph1103I ATGCAT 2 cut(s) 272, 2335
MroI TCCGGA 1 cut(s) 1406
MroXI GAANNNNTTC 2 cut(s) 1112, 1229
MscI TGGCCA 1 cut(s) 405
MseI TTAA 5 cut(s) 107, 282, 581, 725, 1595
MslI CAYNNNNRTG 2 cut(s) 126, 2757
Msp20I TGGCCA 1 cut(s) 405
MspA1I CMGCKG 2 cut(s) 1099, 1692
MspR9I CCNGG 3 cut(s) 504, 842, 2674
MunI CAATTG 3 cut(s) 1637, 2139, 2184
Mva1269I GAATGC 1 cut(s) 2335
MvnI CGCG 1 cut(s) 1463
MwoI GCNNNNNNNGC 8 cut(s) 267, 708, 1597, 1865, 1874, 2006, 2579, 3129
NarI GGCGCC 1 cut(s) 3065
NciI CCSGG 3 cut(s) 504, 842, 2674
NcoI CCATGG 1 cut(s) 3192
NdeI CATATG 1 cut(s) 2335
NlaIV GGNNCC 6 cut(s) 1226, 1433, 1624, 1781, 1858, 3066
NmeAIII GCCGAG 3 cut(s) 1060, 1331, 3149
NmuCI GTSAC 3 cut(s) 416, 2852, 3020
NruI TCGCGA 1 cut(s) 1463
NsbI TGCGCA 1 cut(s) 2875
NsiI ATGCAT 2 cut(s) 272, 2335
NspI RCATGY 3 cut(s) 91, 270, 2453
PaeR7I CTCGAG 1 cut(s) 574
PceI AGGCCT 1 cut(s) 3112
PcsI WCGNNNNNNNCGW 5 cut(s) 375, 1506, 2352, 2715, 2985
PctI GAATGC 1 cut(s) 2335
PdmI GAANNNNTTC 2 cut(s) 1112, 1229
PfeI GAWTC 8 cut(s) 799, 982, 1018, 1054, 1126, 1796, 2800, 3035
PflMI CCANNNNNTGG 1 cut(s) 1971
PfoI TCCNGGA 1 cut(s) 840
PkrI GCNGC 9 cut(s) 1086, 1505, 1679, 1694, 1910, 2008, 2161, 2957, 3210
PleI GAGTC 5 cut(s) 839, 1501, 2547, 2552, 2894
PluTI GGCGCC 1 cut(s) 3068
PmaCI CACGTG 2 cut(s) 1968, 2791
PmlI CACGTG 2 cut(s) 1968, 2791
PpsI GAGTC 5 cut(s) 839, 1501, 2547, 2552, 2894
Ppu21I YACGTR 4 cut(s) 22, 1968, 2791, 3252
PsiI TTATAA 2 cut(s) 32, 3174
Psp124BI GAGCTC 3 cut(s) 870, 1883, 2437
PspCI CACGTG 2 cut(s) 1968, 2791
PspEI GGTNACC 1 cut(s) 2852
PspN4I GGNNCC 6 cut(s) 1226, 1433, 1624, 1781, 1858, 3066
PspPI GGNCC 9 cut(s) 905, 1432, 1622, 1663, 1694, 2766, 2938, 2971, 3145
PvuII CAGCTG 1 cut(s) 1099
RruI TCGCGA 1 cut(s) 1463
RsaI GTAC 4 cut(s) 659, 1200, 2845, 3250
RsaNI GTAC 4 cut(s) 658, 1199, 2844, 3249
RseI CAYNNNNRTG 2 cut(s) 126, 2757
SacI GAGCTC 3 cut(s) 870, 1883, 2437
SaqAI TTAA 5 cut(s) 107, 282, 581, 725, 1595
SatI GCNGC 9 cut(s) 1085, 1504, 1678, 1693, 1909, 2007, 2160, 2956, 3209
Sau96I GGNCC 9 cut(s) 905, 1432, 1622, 1663, 1694, 2766, 2938, 2971, 3145
ScaI AGTACT 1 cut(s) 659
SchI GAGTC 5 cut(s) 839, 1502, 2548, 2552, 2894
ScrFI CCNGG 3 cut(s) 504, 842, 2674
SduI GDGCHC 6 cut(s) 870, 1883, 2118, 2203, 2437, 2731
SfaNI GCATC 6 cut(s) 279, 1578, 1710, 2212, 2724, 2749
SfoI GGCGCC 1 cut(s) 3066
Sfr274I CTCGAG 1 cut(s) 574
SinI GGWCC 5 cut(s) 905, 1622, 2766, 2971, 3145
SlaI CTCGAG 1 cut(s) 574
SmiMI CAYNNNNRTG 2 cut(s) 126, 2757
SmlI CTYRAG 4 cut(s) 214, 433, 574, 1013
SmoI CTYRAG 4 cut(s) 214, 433, 574, 1013
SpeI ACTAGT 1 cut(s) 71
SseBI AGGCCT 1 cut(s) 3112
SsiI CCGC 9 cut(s) 614, 908, 1367, 1678, 1692, 1908, 2383, 2955, 3143
SspDI GGCGCC 1 cut(s) 3064
SspI AATATT 2 cut(s) 1816, 2351
SspMI CTAG 9 cut(s) 72, 518, 851, 1667, 1784, 2651, 2831, 2865, 2897
SstI GAGCTC 3 cut(s) 870, 1883, 2437
StuI AGGCCT 1 cut(s) 3112
StyD4I CCNGG 3 cut(s) 502, 840, 2672
StyI CCWWGG 3 cut(s) 1783, 2562, 3192
TaaI ACNGT 6 cut(s) 1162, 2074, 2442, 2843, 3029, 3119
TaqII GACCGA 2 cut(s) 1721, 2783
TatI WGTACW 2 cut(s) 657, 2843
TauI GCSGC 4 cut(s) 1680, 1695, 1911, 2958
TfiI GAWTC 8 cut(s) 799, 982, 1018, 1054, 1126, 1796, 2800, 3035
Tru1I TTAA 5 cut(s) 107, 282, 581, 725, 1595
Tru9I TTAA 5 cut(s) 107, 282, 581, 725, 1595
TscAI CASTG 6 cut(s) 16, 238, 1165, 2626, 2846, 2866
TseFI GTSAC 3 cut(s) 416, 2852, 3020
TseI GCWGC 5 cut(s) 1084, 1503, 2006, 2159, 3208
Tsp45I GTSAC 3 cut(s) 416, 2852, 3020
TspDTI ATGAA 7 cut(s) 1013, 1635, 2097, 2823, 2840, 2895, 3108
TspGWI ACGGA 5 cut(s) 903, 1792, 1851, 2422, 3008
TspRI CASTG 6 cut(s) 16, 238, 1165, 2626, 2846, 2866
Van91I CCANNNNNTGG 1 cut(s) 1971
VneI GTGCAC 1 cut(s) 2199
VpaK11BI GGWCC 5 cut(s) 905, 1622, 2766, 2971, 3145
XceI RCATGY 3 cut(s) 91, 270, 2453
XcmI CCANNNNNNNNNTGG 1 cut(s) 488
XhoI CTCGAG 1 cut(s) 574
XmaJI CCTAGG 1 cut(s) 1783
XmiI GTMKAC 2 cut(s) 1167, 2037
XmnI GAANNNNTTC 2 cut(s) 1112, 1229
XspI CTAG 9 cut(s) 72, 518, 851, 1667, 1784, 2651, 2831, 2865, 2897
ZraI GACGTC 1 cut(s) 3007
ZrmI AGTACT 1 cut(s) 659
Zsp2I ATGCAT 2 cut(s) 272, 2335
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.