pycom08g09350
NAC Family

Probably inactive leucine-rich repeat receptor-like protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0008421
Physical Location & Seq
Forward (+)
7616164 .. 7619309
3146 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g09350.1

Sequence Viewer

Length: 2946 bp
ATGATTAAGATGGAAAAGGGACAACCTCATCAAACACGTCTCTTATGGTGCATTCTCGTGTTCTTATTCTTCTTCTCAAATGATACTTGTGCATTGGATGGTATTGATGAGCTTCAGCTTCTCTTATCCTTCAAAGCTTATATCAACGACCCCTTACACTACCTCTCAAACTGGAACACTTCAGCCAACACTTTATGCAATTGGCATGGCATCACATGCAACGACAACAACAACATCAAAGCCATCGAGCTCCGCGGAAGAAACATCTCTGGCAAGATATCATCCTCCATCTTCCACTTGTCCCAAGTCGAAACTATCGACCTCTCCAACAATCAGCTATCCGGCCAAGTCCCCAAAGACGTGTTTATAAACGGGTCTAATTCGCTTCGCCATCTCAACTTCAGCAGCAACAACTTAACCGGCACAGTTCCACAAGGCTCACTCACAACCCTAGAGGTACTAGACTTGTCCAACAACATGATTTCAGGCACAATCCCAAATGACATCGGATCGTTTTCGAGCTTGAAATTTCTTGACCTTGGAGGAAATGTGTTGGTGGGAAACATCCCTAGTTCCATATCAAACCTGTCTAGCTTGGAGTACTTAACTTTGGCCTCAAACCAACTTGTTGGAAGAATTCCTACACAATTAGGCCAAATGAAGAACTTGAAGTGGATCTACTTGGGCTACAACAGTCTCTCCGGCGAAATCCCTGTACAAATTGGTAACCTTTTTGGCTTGAATCATCTCGATCTTGTTTACAACAACCTCACCGGCGAAATCCCATACACAATCAGCAACCTTTCTAATCTTCAGTACCTTTTCCTTTATGGAAACAAACTCAGAGGTCCAGTTCCACAATCCTTATTCGGTCTCGAGAAACTCGTTTCTCTCGACCTTAGTGACAATTTTCTTTCGGGAGAGATACCGGAAAGTGTTTTCCAACTCCAAAACTTGGAAATCCTCCACCTTTTTTCAAACAACTTCACAGGAAAAATTCCCAACGCTTTGGCTTCTCTGCCAAGGCTTCAAGTCCTTCAGTTATGGTCAAACAAGTTCTCAGGCCAAATCCCGAAACGTCTCGGAAGCCAAAACAACCTCACTGTCTTAGACCTTTCCTCAAACAACCTTTCTGGAAAAATACCTGATACGCTATGCGACTCAGGCCGGCTTTTTAAGCTCATCCTCTTCTCCAATTCACTCGAAGGCGAAATCCCCCGAAGTTTGTCTTTTTGCAAGAGCTTGAGCCGAGTAAGACTCCAGAACAACCGGCTTTCCGGTGAAATATTGGCCGAGTTCACCAAACTCCCTCTTGTCTATTTCCTGGATATCTCGGGCAACAATCTCTCTGGCAGAATCGATGACAAAAAGTGGGACATGCCCTCTCTTCAAATGCTGAATATGGCGAGAAATCGATTTTTCGGGAAGTTACCGGCAAATTTTGGAAGCGAAAAGCTCGAGAACTTGGACTTGTCCGAAAATTGGTTATCGGGAACTATCTCACTGAGTTTCCGGAACTTGTCGGAACTAATGCAGTTGAAGCTTTCTCGCAATGAACTCTCTGGTCCCATCCCTCAACAATTGTCTTCATGCAAAAAACTCGTGAGCCTCGACCTCAGCCACAACCGCCTCACCGGCACGATTCCCACTAGCCTCTCTGATATGCCGGTTCTCGGGGACCTAGATTTATCCGAAAACCAAATATCGGGCGAGATTCCACGGAATTTGGGAGCTATAGTATCGCTTGTTCAAGTCAACATTTCCCACAACAAACTCCACGGGATGTTGCCGCCCACAGCAGCGTTTCTTGCCATAAATGCCAGCGCAGTGGACGGCAACAACCTCTGCGGCGGAAGTGACACCATTAGTGGTCTACCTCCGTGCAAGGGCGTTAAGAGAAACCCTACTTGGTGGTTTATTGTCACTTGTTTTCTTGTTGCATTATTGGCTTTTGGAGTCGCCGGTTATTTATTTGTGTTAATGAGGAGGAGGAGAAAGGATTTGGAGGTTAAAACGGTGGAGAGCAAAGATGGGATTTGGGAGCTGCAGTTTTTCGAATCGAGGGTTTCTAGATCGGTTACAATTCATGACATTTTATCGGCAGCCAAAGAAGGTAATATCATTGCGATGGAAAAGACTGGGATTTTATATAAAGGAGAATCAGTTTCCAACGGAATGCAATTCGTGGTAAAAGAGGATTCTGTGAAAGCAATTCCACCAAGTTTATTGTCACAGATTGTTGCACTTGGGAAGCTTAGGCACCCCAATGTGATCAAACTGATCGGAATATGTCATTCCGAAAATGGTGCGTATGTGCTCTATGAGTATTATGAAGGGAAAGTGTTGGGTCAAGTTATGAGGGATTTGAGTTGGGATCAGCGCCGGAAAATTGCTGTTGGAATTGCGAAGGCTCTACGGTTCTTGCACTGTTGTTGCTCGCCGAGCGTTGTAGCTGGTTGTGTGTCGGCAGAGAAAGTGATTGTGGACGCAAAAGATGAGCCTCGAATTCGATTGAGCCTCTCCGAACAAGTTCGGACGGACTCCAAGGGATTCGTTGTTTCAGCATACATTGCTCCGGAAGACAAAGAAAGCAAAGCCGGCATTACAGAGAAGACTGACATCTATGGGTTTGGGCTCGTCTTGATCGAATTGCTAACCGGAAAAGGCCCCAGTGACACTGAATTTGGCGCGCACCAGAGCGTCATCGAGTGGGCTCGGTACTGCTACTCAGACTGCCATCTTGATGTCTGGACCGACTCGATGATCAGAGAACACGTGTCGAGCAATCAGAACGAGATTGTGGAAACCATGAACCTAGCCCTTCACTGCACTGCTAGTGACCCCATGGCTAGACCATGCGCGAATGAAATATATAAAACCCTAGAATCTGTAATGAGGACAAGTAGTTCTTGTGTTCCTGGATTAAAAGTTACTTCACCTTTTTGA

Protein Analysis

982

Amino Acids

108.39

Weight (kDa)

6.82

Isoelectric Point (pI)

32.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 36 - 74 5.2e-11 Leucine rich repeat N-terminal domain
LRR_14 PF23598 129 - 224 1.5e-07 Leucine-rich repeat region
LRR_14 PF23598 129 - 254 2.1e-10 Leucine-rich repeat region
LRR_8 PF13855 246 - 303 5.1e-09 Leucine rich repeat
LRR_14 PF23598 265 - 388 1e-11 Leucine-rich repeat region
LRR_8 PF13855 342 - 401 7.1e-06 Leucine rich repeat
LRR_8 PF13855 485 - 544 1.4e-08 Leucine rich repeat
LRR_8 PF13855 533 - 592 4.6e-08 Leucine rich repeat
Pkinase PF00069 714 - 959 2e-17 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 724 - 961 1.1e-16 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 368
AccB1I GGYRCC 1 cut(s) 2259
AccB7I CCANNNNNTGG 1 cut(s) 955
AccI GTMKAC 1 cut(s) 1873
AccII CGCG 3 cut(s) 255, 2690, 2861
AccIII TCCGGA 2 cut(s) 1512, 2575
AciI CCGC 6 cut(s) 253, 255, 1627, 1790, 1848, 1851
AclWI GGATC 3 cut(s) 517, 683, 2382
AcoI YGGCCR 2 cut(s) 343, 1290
AcsI RAATTY 7 cut(s) 527, 636, 996, 1438, 1723, 2505, 2681
AcuI CTGAAG 5 cut(s) 98, 165, 385, 797, 1022
AcvI CACGTG 1 cut(s) 2776
AfaI GTAC 5 cut(s) 459, 602, 717, 818, 2720
AfiI CCNNNNNNNGG 5 cut(s) 955, 1482, 1673, 1705, 1886
AflIII ACRYGT 4 cut(s) 35, 360, 2773, 2775
AgsI TTSAA 9 cut(s) 133, 526, 670, 742, 978, 1031, 1391, 1540, 1751
AjiI CACGTC 2 cut(s) 38, 361
AjnI CCWGG 2 cut(s) 1323, 2917
AjuI GAANNNNNNNTTGG 2 cut(s) 1891, 1923
AloI GAACNNNNNNTCC 2 cut(s) 2890, 2922
Alw21I GWGCWC 2 cut(s) 252, 2319
Alw26I GTCTC 4 cut(s) 44, 701, 878, 1085
AlwI GGATC 3 cut(s) 517, 683, 2382
Ama87I CYCGRG 4 cut(s) 875, 1333, 1457, 1673
Aor13HI TCCGGA 2 cut(s) 1512, 2575
AoxI GGCC 7 cut(s) 343, 612, 652, 1063, 1165, 1290, 2665
ApeKI GCWGC 4 cut(s) 405, 1799, 2044, 2102
ApoI RAATTY 7 cut(s) 527, 636, 996, 1438, 1723, 2505, 2681
ArsI GACNNNNNNTTYG 2 cut(s) 2666, 2698
Asp700I GAANNNNTTC 1 cut(s) 2529
AspLEI GCGC 5 cut(s) 1826, 2382, 2690, 2692, 2861
AspS9I GGNCC 5 cut(s) 848, 1565, 1678, 2666, 2751
AsuHPI GGTGA 5 cut(s) 763, 1291, 1292, 1624, 2928
AsuII TTCGAA 1 cut(s) 2055
AvaI CYCGRG 4 cut(s) 875, 1333, 1457, 1673
AvaII GGWCC 4 cut(s) 848, 1565, 1678, 2751
BanI GGYRCC 1 cut(s) 2259
BanII GRGCYC 3 cut(s) 252, 2637, 2716
BarI GAAGNNNNNNTAC 4 cut(s) 625, 657, 662, 694
BauI CACGAG 2 cut(s) 56, 1601
BbrPI CACGTG 1 cut(s) 2776
BbsI GAAGAC 3 cut(s) 1578, 2586, 2618
Bbv12I GWGCWC 2 cut(s) 252, 2319
BbvCI CCTCAGC 1 cut(s) 1616
BbvI GCAGC 4 cut(s) 417, 1811, 2031, 2114
BccI CCATC 9 cut(s) 4, 92, 251, 296, 399, 1577, 2024, 2122, 2745
BceAI ACGGC 1 cut(s) 1849
BcgI CGANNNNNNTGC 4 cut(s) 1582, 1616, 2288, 2322
BciT130I CCWGG 2 cut(s) 1325, 2919
BclI TGATCA 2 cut(s) 2271, 2763
BcoDI GTCTC 4 cut(s) 44, 701, 878, 1085
BfmI CTRYAG 2 cut(s) 1734, 2045
BfoI RGCGCY 1 cut(s) 2383
BglI GCCNNNNNGGC 1 cut(s) 1635
BisI GCNGC 6 cut(s) 406, 1790, 1800, 1849, 2045, 2103
BlsI GCNGC 6 cut(s) 407, 1791, 1801, 1850, 2046, 2104
BmcAI AGTACT 1 cut(s) 602
Bme1390I CCNGG 2 cut(s) 1325, 2919
Bme18I GGWCC 4 cut(s) 848, 1565, 1678, 2751
BmeT110I CYCGRG 4 cut(s) 875, 1333, 1457, 1673
BmgBI CACGTC 2 cut(s) 38, 361
BmgT120I GGNCC 5 cut(s) 848, 1565, 1678, 2666, 2751
BmiI GGNNCC 4 cut(s) 1567, 1679, 2261, 2668
BmrFI CCNGG 2 cut(s) 1325, 2919
BmrI ACTGGG 2 cut(s) 2148, 2664
BmsI GCATC 1 cut(s) 219
BmuI ACTGGG 2 cut(s) 2148, 2664
BpiI GAAGAC 3 cut(s) 1578, 2586, 2618
BplI GAGNNNNNCTC 2 cut(s) 1242, 1274
BpmI CTGGAG 1 cut(s) 1244
Bpu10I CCTNAGC 2 cut(s) 1616, 2255
Bpu14I TTCGAA 1 cut(s) 2055
BpuEI CTTGAG 1 cut(s) 1264
Bsa29I ATCGAT 2 cut(s) 1359, 1414
BsaAI YACGTR 1 cut(s) 2776
BsaI GGTCTC 1 cut(s) 878
BsaJI CCNNGG 7 cut(s) 253, 538, 1022, 1718, 1777, 2544, 2844
BsaWI WCCGGW 5 cut(s) 928, 1277, 1512, 2575, 2657
Bsc4I CCNNNNNNNGG 5 cut(s) 955, 1482, 1673, 1705, 1886
Bse118I RCCGGY 9 cut(s) 419, 773, 1167, 1269, 1432, 1634, 1666, 1961, 2597
Bse1I ACTGG 4 cut(s) 176, 851, 2143, 2670
Bse3DI GCAATG 3 cut(s) 1558, 2121, 2568
BseAI TCCGGA 2 cut(s) 1512, 2575
BseBI CCWGG 2 cut(s) 1325, 2919
BseCI ATCGAT 2 cut(s) 1359, 1414
BseDI CCNNGG 7 cut(s) 253, 538, 1022, 1718, 1777, 2544, 2844
BseGI GGATG 6 cut(s) 103, 281, 564, 1182, 1569, 1788
BseLI CCNNNNNNNGG 5 cut(s) 955, 1482, 1673, 1705, 1886
BseMI GCAATG 3 cut(s) 1558, 2121, 2568
BseMII CTCAG 6 cut(s) 856, 1074, 1176, 1496, 1630, 2742
BseNI ACTGG 4 cut(s) 176, 851, 2143, 2670
BsePI GCGCGC 1 cut(s) 2688
BseRI GAGGAG 3 cut(s) 1999, 2002, 2005
BseXI GCAGC 4 cut(s) 417, 1811, 2031, 2114
BsgI GTGCAG 1 cut(s) 2812
Bsh1236I CGCG 3 cut(s) 255, 2690, 2861
BshFI GGCC 7 cut(s) 345, 614, 654, 1065, 1167, 1292, 2667
BshNI GGYRCC 1 cut(s) 2259
BshVI ATCGAT 2 cut(s) 1359, 1414
BsiHKAI GWGCWC 2 cut(s) 252, 2319
BsiHKCI CYCGRG 4 cut(s) 875, 1333, 1457, 1673
BslFI GGGAC 6 cut(s) 33, 286, 335, 1388, 1551, 1691
BslI CCNNNNNNNGG 5 cut(s) 955, 1482, 1673, 1705, 1886
BsmAI GTCTC 4 cut(s) 44, 701, 878, 1085
BsmBI CGTCTC 2 cut(s) 44, 1085
BsmFI GGGAC 6 cut(s) 33, 286, 335, 1388, 1551, 1691
BsmI GAATGC 2 cut(s) 51, 2181
BsnI GGCC 7 cut(s) 345, 614, 654, 1065, 1167, 1292, 2667
Bso31I GGTCTC 1 cut(s) 878
BsoBI CYCGRG 4 cut(s) 875, 1333, 1457, 1673
Bsp119I TTCGAA 1 cut(s) 2055
Bsp1286I GDGCHC 4 cut(s) 252, 2319, 2637, 2716
Bsp13I TCCGGA 2 cut(s) 1512, 2575
Bsp1407I TGTACA 1 cut(s) 715
Bsp143I GATC 9 cut(s) 509, 675, 751, 2072, 2271, 2280, 2374, 2643, 2763
Bsp19I CCATGG 1 cut(s) 2844
BspACI CCGC 6 cut(s) 253, 255, 1627, 1790, 1848, 1851
BspANI GGCC 7 cut(s) 345, 614, 654, 1065, 1167, 1292, 2667
BspCNI CTCAG 6 cut(s) 855, 1073, 1175, 1497, 1629, 2741
BspDI ATCGAT 2 cut(s) 1359, 1414
BspEI TCCGGA 2 cut(s) 1512, 2575
BspFNI CGCG 3 cut(s) 255, 2690, 2861
BspHI TCATGA 1 cut(s) 2086
BspLI GGNNCC 4 cut(s) 1567, 1679, 2261, 2668
BspMAI CTGCAG 1 cut(s) 2049
BspPI GGATC 3 cut(s) 517, 683, 2382
BspT104I TTCGAA 1 cut(s) 2055
BspT107I GGYRCC 1 cut(s) 2259
BspTNI GGTCTC 1 cut(s) 878
BsrDI GCAATG 3 cut(s) 1558, 2121, 2568
BsrFI RCCGGY 9 cut(s) 419, 773, 1167, 1269, 1432, 1634, 1666, 1961, 2597
BsrGI TGTACA 1 cut(s) 715
BsrI ACTGG 4 cut(s) 176, 851, 2143, 2670
BssAI RCCGGY 9 cut(s) 419, 773, 1167, 1269, 1432, 1634, 1666, 1961, 2597
BssECI CCNNGG 7 cut(s) 253, 538, 1022, 1718, 1777, 2544, 2844
BssHII GCGCGC 1 cut(s) 2688
BssMI GATC 9 cut(s) 509, 675, 751, 2072, 2271, 2280, 2374, 2643, 2763
BssSI CACGAG 2 cut(s) 56, 1601
BssT1I CCWWGG 4 cut(s) 538, 1022, 2544, 2844
Bst2BI CACGAG 2 cut(s) 56, 1601
Bst2UI CCWGG 2 cut(s) 1325, 2919
Bst4CI ACNGT 6 cut(s) 427, 695, 1105, 2017, 2418, 2429
Bst6I CTCTTC 2 cut(s) 1193, 1392
BstAPI GCANNNNNTGC 2 cut(s) 216, 2570
BstAUI TGTACA 1 cut(s) 715
BstBAI YACGTR 1 cut(s) 2776
BstBI TTCGAA 1 cut(s) 2055
BstC8I GCNNGC 5 cut(s) 1169, 1822, 2438, 2599, 2690
BstDEI CTNAG 9 cut(s) 842, 899, 1060, 1108, 1162, 1505, 1616, 2255, 2728
BstDSI CCRYGG 4 cut(s) 253, 1718, 1777, 2844
BstEII GGTNACC 1 cut(s) 725
BstF5I GGATG 6 cut(s) 103, 281, 564, 1182, 1569, 1788
BstFNI CGCG 3 cut(s) 255, 2690, 2861
BstH2I RGCGCY 1 cut(s) 2383
BstHHI GCGC 5 cut(s) 1826, 2382, 2690, 2692, 2861
BstKTI GATC 9 cut(s) 512, 678, 754, 2075, 2274, 2283, 2377, 2646, 2766
BstMAI GTCTC 4 cut(s) 44, 701, 878, 1085
BstMBI GATC 9 cut(s) 509, 675, 751, 2072, 2271, 2280, 2374, 2643, 2763
BstNI CCWGG 2 cut(s) 1325, 2919
BstNSI RCATGY 2 cut(s) 219, 1381
BstPI GGTNACC 1 cut(s) 725
BstSCI CCNGG 2 cut(s) 1323, 2917
BstSFI CTRYAG 2 cut(s) 1734, 2045
BstUI CGCG 3 cut(s) 255, 2690, 2861
BstV1I GCAGC 4 cut(s) 417, 1811, 2031, 2114
BstV2I GAAGAC 3 cut(s) 1578, 2586, 2618
BstX2I RGATCY 1 cut(s) 675
BstXI CCANNNNNNTGG 3 cut(s) 629, 1009, 1828
BstYI RGATCY 1 cut(s) 675
Bsu15I ATCGAT 2 cut(s) 1359, 1414
BsuRI GGCC 7 cut(s) 345, 614, 654, 1065, 1167, 1292, 2667
BsuTUI ATCGAT 2 cut(s) 1359, 1414
BtgI CCRYGG 4 cut(s) 253, 1718, 1777, 2844
BtgZI GCGATG 1 cut(s) 2141
BtrI CACGTC 2 cut(s) 38, 361
BtsCI GGATG 6 cut(s) 103, 281, 564, 1182, 1569, 1788
BtsI GCAGTG 3 cut(s) 1833, 2824, 2829
BtsIMutI CAGTG 8 cut(s) 1101, 1502, 1833, 2425, 2676, 2677, 2824, 2829
Cac8I GCNNGC 5 cut(s) 1169, 1822, 2438, 2599, 2690
CciI TCATGA 1 cut(s) 2086
CfoI GCGC 5 cut(s) 1826, 2382, 2690, 2692, 2861
Cfr10I RCCGGY 9 cut(s) 419, 773, 1167, 1269, 1432, 1634, 1666, 1961, 2597
Cfr13I GGNCC 5 cut(s) 848, 1565, 1678, 2666, 2751
Cfr42I CCGCGG 1 cut(s) 256
ClaI ATCGAT 2 cut(s) 1359, 1414
CseI GACGC 2 cut(s) 2495, 2689
Csp6I GTAC 5 cut(s) 458, 601, 716, 817, 2719
CspCI CAANNNNNGTGG 2 cut(s) 1707, 1742
CviAII CATG 9 cut(s) 206, 216, 478, 1378, 1590, 2087, 2809, 2845, 2856
CviQI GTAC 5 cut(s) 458, 601, 716, 817, 2719
DdeI CTNAG 9 cut(s) 842, 899, 1060, 1108, 1162, 1505, 1616, 2255, 2728
DpnI GATC 9 cut(s) 511, 677, 753, 2074, 2273, 2282, 2376, 2645, 2765
DpnII GATC 9 cut(s) 509, 675, 751, 2072, 2271, 2280, 2374, 2643, 2763
EaeI YGGCCR 2 cut(s) 343, 1290
Eam1104I CTCTTC 2 cut(s) 1193, 1392
EarI CTCTTC 2 cut(s) 1193, 1392
EciI GGCGGA 1 cut(s) 1866
Ecl136II GAGCTC 1 cut(s) 250
Eco130I CCWWGG 4 cut(s) 538, 1022, 2544, 2844
Eco24I GRGCYC 3 cut(s) 252, 2637, 2716
Eco31I GGTCTC 1 cut(s) 878
Eco32I GATATC 2 cut(s) 279, 1330
Eco47I GGWCC 4 cut(s) 848, 1565, 1678, 2751
Eco53kI GAGCTC 1 cut(s) 250
Eco57I CTGAAG 5 cut(s) 98, 165, 385, 797, 1022
Eco72I CACGTG 1 cut(s) 2776
Eco88I CYCGRG 4 cut(s) 875, 1333, 1457, 1673
Eco91I GGTNACC 1 cut(s) 725
EcoICRI GAGCTC 1 cut(s) 250
EcoO109I RGGNCCY 2 cut(s) 1678, 2666
EcoO65I GGTNACC 1 cut(s) 725
EcoRI GAATTC 2 cut(s) 636, 2505
EcoRII CCWGG 2 cut(s) 1323, 2917
EcoRV GATATC 2 cut(s) 279, 1330
EcoT14I CCWWGG 4 cut(s) 538, 1022, 2544, 2844
EcoT38I GRGCYC 3 cut(s) 252, 2637, 2716
ErhI CCWWGG 4 cut(s) 538, 1022, 2544, 2844
Esp3I CGTCTC 2 cut(s) 44, 1085
FaeI CATG 9 cut(s) 209, 219, 481, 1381, 1593, 2090, 2812, 2848, 2859
FalI AAGNNNNNCTT 4 cut(s) 1213, 1245, 2893, 2925
FaqI GGGAC 6 cut(s) 33, 286, 335, 1388, 1551, 1691
FatI CATG 9 cut(s) 205, 215, 477, 1377, 1589, 2086, 2808, 2844, 2855
FbaI TGATCA 2 cut(s) 2271, 2763
FblI GTMKAC 1 cut(s) 1873
Fnu4HI GCNGC 6 cut(s) 406, 1790, 1800, 1849, 2045, 2103
FokI GGATG 6 cut(s) 110, 268, 551, 1169, 1556, 1795
FriOI GRGCYC 3 cut(s) 252, 2637, 2716
Fsp4HI GCNGC 6 cut(s) 406, 1790, 1800, 1849, 2045, 2103
GlaI GCGC 5 cut(s) 1825, 2381, 2689, 2691, 2860
GluI GCNGC 6 cut(s) 406, 1790, 1800, 1849, 2045, 2103
GsuI CTGGAG 1 cut(s) 1244
HaeII RGCGCY 1 cut(s) 2383
HaeIII GGCC 7 cut(s) 345, 614, 654, 1065, 1167, 1292, 2667
HgaI GACGC 2 cut(s) 2495, 2689
HhaI GCGC 5 cut(s) 1826, 2382, 2690, 2692, 2861
Hin1II CATG 9 cut(s) 209, 219, 481, 1381, 1593, 2090, 2812, 2848, 2859
Hin6I GCGC 5 cut(s) 1824, 2380, 2688, 2690, 2859
HinP1I GCGC 5 cut(s) 1824, 2380, 2688, 2690, 2859
HincII GTYRAC 1 cut(s) 1756
HindII GTYRAC 1 cut(s) 1756
HindIII AAGCTT 3 cut(s) 135, 1541, 2252
HphI GGTGA 5 cut(s) 763, 1291, 1292, 1624, 2928
Hpy166II GTNNAC 6 cut(s) 760, 1299, 1756, 1831, 1874, 2485
Hpy8I GTNNAC 6 cut(s) 760, 1299, 1756, 1831, 1874, 2485
HpyAV CCTTC 7 cut(s) 139, 1046, 1199, 2105, 2327, 2401, 2831
HpyCH4III ACNGT 6 cut(s) 427, 695, 1105, 2017, 2418, 2429
HpyCH4IV ACGT 4 cut(s) 37, 360, 1078, 2775
HpyF3I CTNAG 9 cut(s) 842, 899, 1060, 1108, 1162, 1505, 1616, 2255, 2728
HpySE526I ACGT 4 cut(s) 37, 360, 1078, 2775
Hsp92II CATG 9 cut(s) 209, 219, 481, 1381, 1593, 2090, 2812, 2848, 2859
HspAI GCGC 5 cut(s) 1824, 2380, 2688, 2690, 2859
Kpn2I TCCGGA 2 cut(s) 1512, 2575
KroI GCCGGC 2 cut(s) 1167, 2597
KroNI GCCGGC 2 cut(s) 1169, 2599
Ksp22I TGATCA 2 cut(s) 2271, 2763
KspI CCGCGG 1 cut(s) 256
Kzo9I GATC 9 cut(s) 509, 675, 751, 2072, 2271, 2280, 2374, 2643, 2763
LmnI GCTCC 4 cut(s) 255, 1730, 2041, 2578
Lsp1109I GCAGC 4 cut(s) 417, 1811, 2031, 2114
LweI GCATC 1 cut(s) 219
MaeII ACGT 4 cut(s) 37, 360, 1078, 2775
MalI GATC 9 cut(s) 511, 677, 753, 2074, 2273, 2282, 2376, 2645, 2765
MboI GATC 9 cut(s) 509, 675, 751, 2072, 2271, 2280, 2374, 2643, 2763
MfeI CAATTG 2 cut(s) 199, 1580
MflI RGATCY 1 cut(s) 675
MhlI GDGCHC 4 cut(s) 252, 2319, 2637, 2716
MlyI GAGTC 5 cut(s) 1154, 1251, 1965, 2534, 2750
MmeI TCCRAC 7 cut(s) 351, 495, 610, 967, 1503, 2193, 2377
MroI TCCGGA 2 cut(s) 1512, 2575
MroNI GCCGGC 2 cut(s) 1167, 2597
MroXI GAANNNNTTC 1 cut(s) 2529
MseI TTAA 8 cut(s) 6, 416, 605, 1176, 1893, 1979, 2010, 2924
MslI CAYNNNNRTG 4 cut(s) 56, 2126, 2265, 2742
MspA1I CMGCKG 1 cut(s) 255
MspR9I CCNGG 2 cut(s) 1325, 2919
MunI CAATTG 2 cut(s) 199, 1580
Mva1269I GAATGC 2 cut(s) 51, 2181
MvaI CCWGG 2 cut(s) 1325, 2919
MvnI CGCG 3 cut(s) 255, 2690, 2861
NaeI GCCGGC 2 cut(s) 1169, 2599
NcoI CCATGG 1 cut(s) 2844
NdeII GATC 9 cut(s) 509, 675, 751, 2072, 2271, 2280, 2374, 2643, 2763
NgoMIV GCCGGC 2 cut(s) 1167, 2597
NlaIII CATG 9 cut(s) 209, 219, 481, 1381, 1593, 2090, 2812, 2848, 2859
NlaIV GGNNCC 4 cut(s) 1567, 1679, 2261, 2668
NmeAIII GCCGAG 3 cut(s) 1274, 1318, 2466
NmuCI GTSAC 6 cut(s) 902, 1856, 1921, 2229, 2672, 2837
NspI RCATGY 2 cut(s) 219, 1381
NspV TTCGAA 1 cut(s) 2055
PaeR7I CTCGAG 2 cut(s) 875, 1457
PagI TCATGA 1 cut(s) 2086
PauI GCGCGC 1 cut(s) 2688
PcsI WCGNNNNNNNCGW 5 cut(s) 315, 882, 891, 1608, 2643
PctI GAATGC 2 cut(s) 51, 2181
PdiI GCCGGC 2 cut(s) 1169, 2599
PdmI GAANNNNTTC 1 cut(s) 2529
PfeI GAWTC 9 cut(s) 742, 1356, 1642, 1714, 2057, 2159, 2198, 2550, 2885
PflMI CCANNNNNTGG 1 cut(s) 955
PfoI TCCNGGA 2 cut(s) 1323, 2917
PkrI GCNGC 6 cut(s) 407, 1791, 1801, 1850, 2046, 2104
PleI GAGTC 5 cut(s) 1154, 1251, 1964, 2534, 2750
PmaCI CACGTG 1 cut(s) 2776
PmlI CACGTG 1 cut(s) 2776
PpsI GAGTC 5 cut(s) 1154, 1251, 1964, 2534, 2750
Ppu21I YACGTR 1 cut(s) 2776
PpuMI RGGWCCY 1 cut(s) 1678
PsiI TTATAA 1 cut(s) 368
Psp124BI GAGCTC 1 cut(s) 252
Psp5II RGGWCCY 1 cut(s) 1678
Psp6I CCWGG 2 cut(s) 1323, 2917
PspCI CACGTG 1 cut(s) 2776
PspEI GGTNACC 1 cut(s) 725
PspGI CCWGG 2 cut(s) 1323, 2917
PspN4I GGNNCC 4 cut(s) 1567, 1679, 2261, 2668
PspPI GGNCC 5 cut(s) 848, 1565, 1678, 2666, 2751
PspPPI RGGWCCY 1 cut(s) 1678
PsrI GAACNNNNNNTAC 2 cut(s) 1731, 1763
PstI CTGCAG 1 cut(s) 2049
PsuI RGATCY 1 cut(s) 675
PteI GCGCGC 1 cut(s) 2688
RsaI GTAC 5 cut(s) 459, 602, 717, 818, 2720
RsaNI GTAC 5 cut(s) 458, 601, 716, 817, 2719
RseI CAYNNNNRTG 4 cut(s) 56, 2126, 2265, 2742
SacI GAGCTC 1 cut(s) 252
SacII CCGCGG 1 cut(s) 256
SaqAI TTAA 8 cut(s) 6, 416, 605, 1176, 1893, 1979, 2010, 2924
SatI GCNGC 6 cut(s) 406, 1790, 1800, 1849, 2045, 2103
Sau3AI GATC 9 cut(s) 509, 675, 751, 2072, 2271, 2280, 2374, 2643, 2763
Sau96I GGNCC 5 cut(s) 848, 1565, 1678, 2666, 2751
ScaI AGTACT 1 cut(s) 602
SchI GAGTC 5 cut(s) 1154, 1251, 1965, 2534, 2750
ScrFI CCNGG 2 cut(s) 1325, 2919
SduI GDGCHC 4 cut(s) 252, 2319, 2637, 2716
SfaNI GCATC 1 cut(s) 219
SfcI CTRYAG 2 cut(s) 1734, 2045
Sfr274I CTCGAG 2 cut(s) 875, 1457
Sfr303I CCGCGG 1 cut(s) 256
SfuI TTCGAA 1 cut(s) 2055
SgrAI CRCCGGYG 1 cut(s) 773
SgrBI CCGCGG 1 cut(s) 256
SinI GGWCC 4 cut(s) 848, 1565, 1678, 2751
SlaI CTCGAG 2 cut(s) 875, 1457
SmiMI CAYNNNNRTG 4 cut(s) 56, 2126, 2265, 2742
SmlI CTYRAG 3 cut(s) 875, 1243, 1457
SmoI CTYRAG 3 cut(s) 875, 1243, 1457
SsiI CCGC 6 cut(s) 253, 255, 1627, 1790, 1848, 1851
SspI AATATT 1 cut(s) 1287
SstI GAGCTC 1 cut(s) 252
StyD4I CCNGG 2 cut(s) 1323, 2917
StyI CCWWGG 4 cut(s) 538, 1022, 2544, 2844
TaaI ACNGT 6 cut(s) 427, 695, 1105, 2017, 2418, 2429
TaiI ACGT 4 cut(s) 40, 363, 1081, 2778
TaqII GACCGA 2 cut(s) 860, 2768
TatI WGTACW 2 cut(s) 600, 715
TauI GCSGC 2 cut(s) 1792, 1851
TfiI GAWTC 9 cut(s) 742, 1356, 1642, 1714, 2057, 2159, 2198, 2550, 2885
Tru1I TTAA 8 cut(s) 6, 416, 605, 1176, 1893, 1979, 2010, 2924
Tru9I TTAA 8 cut(s) 6, 416, 605, 1176, 1893, 1979, 2010, 2924
TscAI CASTG 8 cut(s) 1108, 1509, 1833, 2432, 2677, 2683, 2831, 2836
TseFI GTSAC 6 cut(s) 902, 1856, 1921, 2229, 2672, 2837
TseI GCWGC 4 cut(s) 405, 1799, 2044, 2102
Tsp45I GTSAC 6 cut(s) 902, 1856, 1921, 2229, 2672, 2837
TspDTI ATGAA 7 cut(s) 674, 1569, 1578, 2075, 2346, 2825, 2880
TspGWI ACGGA 4 cut(s) 1735, 1869, 2187, 2552
TspRI CASTG 8 cut(s) 1108, 1509, 1833, 2432, 2677, 2683, 2831, 2836
Van91I CCANNNNNTGG 1 cut(s) 955
VpaK11BI GGWCC 4 cut(s) 848, 1565, 1678, 2751
XapI RAATTY 7 cut(s) 527, 636, 996, 1438, 1723, 2505, 2681
XbaI TCTAGA 1 cut(s) 2069
XceI RCATGY 2 cut(s) 219, 1381
XhoI CTCGAG 2 cut(s) 875, 1457
XmiI GTMKAC 1 cut(s) 1873
XmnI GAANNNNTTC 1 cut(s) 2529
ZrmI AGTACT 1 cut(s) 602
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.