MD08G1113300.v1.1

isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0009762
Physical Location & Seq
Reverse (-)
10051603 .. 10054240
2638 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1113300.v1.1.491

Sequence Viewer

Length: 279 bp
ATGGCTTCTCGCTGCAGATCTTTCTCGAAGCCGGCTTTCTCTCTTCTCAAATCCGCCGTCAACAAACCGGCGTTCAAGGCCACTCCAATGTCCTCTCTCCCCACCGCCCGCCCTTCTATCAATCTCCCAAGGCCGTTTCCTCAGTTGGGTTGTCTGCAATCTCTGCTTCCATTTCACACGGCGGTGTCTTCAGCCCGGCTCACGTCGTGCCTCGGCATTGACTCGAGGAGCTCGAGGTCGTTGTCTCAGGGTATGCTCGGTGCAAACCCAGGAGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

93

Amino Acids

9.7

Weight (kDa)

11.63

Isoelectric Point (pI)

82.02

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 4 cut(s) 54, 105, 109, 182
AcuI CTGAAG 1 cut(s) 174
AdeI CACNNNGTG 1 cut(s) 207
AfiI CCNNNNNNNGG 1 cut(s) 146
AgsI TTSAA 1 cut(s) 76
AjiI CACGTC 1 cut(s) 204
AjnI CCWGG 1 cut(s) 268
AleI CACNNNNGTG 1 cut(s) 182
AluBI AGCT 1 cut(s) 231
AluI AGCT 1 cut(s) 231
Alw21I GWGCWC 1 cut(s) 233
Alw26I GTCTC 1 cut(s) 249
Ama87I CYCGRG 2 cut(s) 223, 232
AoxI GGCC 2 cut(s) 78, 131
ApeKI GCWGC 1 cut(s) 12
ArsI GACNNNNNNTTYG 2 cut(s) 42, 74
AsuC2I CCSGG 1 cut(s) 196
AvaI CYCGRG 2 cut(s) 223, 232
BanII GRGCYC 1 cut(s) 233
BbsI GAAGAC 1 cut(s) 180
Bbv12I GWGCWC 1 cut(s) 233
BceAI ACGGC 3 cut(s) 41, 118, 195
BciT130I CCWGG 1 cut(s) 270
BcnI CCSGG 1 cut(s) 196
BcoDI GTCTC 1 cut(s) 249
BfmI CTRYAG 1 cut(s) 13
BglII AGATCT 1 cut(s) 17
BisI GCNGC 1 cut(s) 13
BlsI GCNGC 1 cut(s) 14
Bme1390I CCNGG 2 cut(s) 196, 270
BmeT110I CYCGRG 2 cut(s) 223, 232
BmgBI CACGTC 1 cut(s) 204
BmrFI CCNGG 2 cut(s) 196, 270
BpiI GAAGAC 1 cut(s) 180
BpuMI CCSGG 1 cut(s) 196
BsaJI CCNNGG 3 cut(s) 128, 211, 268
Bsc4I CCNNNNNNNGG 1 cut(s) 146
Bse118I RCCGGY 2 cut(s) 31, 67
BseBI CCWGG 1 cut(s) 270
BseDI CCNNGG 3 cut(s) 128, 211, 268
BseLI CCNNNNNNNGG 1 cut(s) 146
BseMII CTCAG 2 cut(s) 155, 260
BseRI GAGGAG 1 cut(s) 241
BshFI GGCC 2 cut(s) 80, 133
BsiHKAI GWGCWC 1 cut(s) 233
BsiHKCI CYCGRG 2 cut(s) 223, 232
BsiSI CCGG 3 cut(s) 32, 68, 196
BslI CCNNNNNNNGG 1 cut(s) 146
BsmAI GTCTC 1 cut(s) 249
BsnI GGCC 2 cut(s) 80, 133
BsoBI CYCGRG 2 cut(s) 223, 232
Bsp1286I GDGCHC 1 cut(s) 233
Bsp143I GATC 1 cut(s) 17
BspACI CCGC 4 cut(s) 54, 105, 109, 182
BspANI GGCC 2 cut(s) 80, 133
BspCNI CTCAG 2 cut(s) 154, 259
BspMAI CTGCAG 1 cut(s) 17
BsrFI RCCGGY 2 cut(s) 31, 67
BssAI RCCGGY 2 cut(s) 31, 67
BssECI CCNNGG 3 cut(s) 128, 211, 268
BssMI GATC 1 cut(s) 17
BssT1I CCWWGG 1 cut(s) 128
Bst2UI CCWGG 1 cut(s) 270
Bst6I CTCTTC 1 cut(s) 48
BstAPI GCANNNNNTGC 1 cut(s) 163
BstC8I GCNNGC 2 cut(s) 33, 109
BstDEI CTNAG 2 cut(s) 141, 246
BstKTI GATC 1 cut(s) 20
BstMAI GTCTC 1 cut(s) 249
BstMBI GATC 1 cut(s) 17
BstMWI GCNNNNNNNGC 2 cut(s) 77, 163
BstNI CCWGG 1 cut(s) 270
BstSCI CCNGG 2 cut(s) 194, 268
BstSFI CTRYAG 1 cut(s) 13
BstV2I GAAGAC 1 cut(s) 180
BstX2I RGATCY 1 cut(s) 17
BstYI RGATCY 1 cut(s) 17
BsuRI GGCC 2 cut(s) 80, 133
BtrI CACGTC 1 cut(s) 204
Cac8I GCNNGC 2 cut(s) 33, 109
Cfr10I RCCGGY 2 cut(s) 31, 67
CviJI RGCY 8 cut(s) 5, 31, 35, 80, 133, 194, 199, 231
CviKI_1 RGCY 8 cut(s) 5, 31, 35, 80, 133, 194, 199, 231
DdeI CTNAG 2 cut(s) 141, 246
DpnI GATC 1 cut(s) 19
DpnII GATC 1 cut(s) 17
DraIII CACNNNGTG 1 cut(s) 207
Eam1104I CTCTTC 1 cut(s) 48
EarI CTCTTC 1 cut(s) 48
EciI GGCGGA 1 cut(s) 43
Ecl136II GAGCTC 1 cut(s) 231
Eco130I CCWWGG 1 cut(s) 128
Eco24I GRGCYC 1 cut(s) 233
Eco53kI GAGCTC 1 cut(s) 231
Eco57I CTGAAG 1 cut(s) 174
Eco88I CYCGRG 2 cut(s) 223, 232
EcoICRI GAGCTC 1 cut(s) 231
EcoRII CCWGG 1 cut(s) 268
EcoT14I CCWWGG 1 cut(s) 128
EcoT38I GRGCYC 1 cut(s) 233
ErhI CCWWGG 1 cut(s) 128
FaiI YATR 1 cut(s) 254
FauI CCCGC 1 cut(s) 116
Fnu4HI GCNGC 1 cut(s) 13
FriOI GRGCYC 1 cut(s) 233
Fsp4HI GCNGC 1 cut(s) 13
GluI GCNGC 1 cut(s) 13
HaeIII GGCC 2 cut(s) 80, 133
HapII CCGG 3 cut(s) 32, 68, 196
HincII GTYRAC 1 cut(s) 61
HindII GTYRAC 1 cut(s) 61
HinfI GANTC 1 cut(s) 221
HpaII CCGG 3 cut(s) 32, 68, 196
Hpy166II GTNNAC 1 cut(s) 61
Hpy188III TCNNGA 1 cut(s) 25
Hpy8I GTNNAC 1 cut(s) 61
Hpy99I CGWCG 1 cut(s) 208
HpyAV CCTTC 1 cut(s) 123
HpyCH4IV ACGT 1 cut(s) 203
HpyCH4V TGCA 3 cut(s) 15, 157, 263
HpyF10VI GCNNNNNNNGC 2 cut(s) 77, 163
HpyF3I CTNAG 2 cut(s) 141, 246
HpySE526I ACGT 1 cut(s) 203
KroI GCCGGC 1 cut(s) 31
KroNI GCCGGC 1 cut(s) 33
Kzo9I GATC 1 cut(s) 17
LmnI GCTCC 1 cut(s) 228
LpnPI CCDG 5 cut(s) 45, 81, 209, 233, 255
MaeII ACGT 1 cut(s) 203
MalI GATC 1 cut(s) 19
MboI GATC 1 cut(s) 17
MboII GAAGA 2 cut(s) 35, 180
MflI RGATCY 1 cut(s) 17
MhlI GDGCHC 1 cut(s) 233
MlyI GAGTC 1 cut(s) 215
MnlI CCTC 5 cut(s) 103, 150, 219, 221, 228
MroNI GCCGGC 1 cut(s) 31
MslI CAYNNNNRTG 2 cut(s) 86, 182
MspI CCGG 3 cut(s) 32, 68, 196
MspR9I CCNGG 2 cut(s) 196, 270
MvaI CCWGG 1 cut(s) 270
MwoI GCNNNNNNNGC 2 cut(s) 77, 163
NaeI GCCGGC 1 cut(s) 33
NciI CCSGG 1 cut(s) 196
NdeII GATC 1 cut(s) 17
NgoMIV GCCGGC 1 cut(s) 31
NmeAIII GCCGAG 1 cut(s) 192
OliI CACNNNNGTG 1 cut(s) 182
PaeR7I CTCGAG 2 cut(s) 223, 232
PcsI WCGNNNNNNNCGW 1 cut(s) 230
PdiI GCCGGC 1 cut(s) 33
PkrI GCNGC 1 cut(s) 14
PleI GAGTC 1 cut(s) 215
PpsI GAGTC 1 cut(s) 215
Psp124BI GAGCTC 1 cut(s) 233
Psp6I CCWGG 1 cut(s) 268
PspGI CCWGG 1 cut(s) 268
PspXI VCTCGAGB 2 cut(s) 223, 232
PstI CTGCAG 1 cut(s) 17
PsuI RGATCY 1 cut(s) 17
RseI CAYNNNNRTG 2 cut(s) 86, 182
SacI GAGCTC 1 cut(s) 233
SatI GCNGC 1 cut(s) 13
Sau3AI GATC 1 cut(s) 17
SchI GAGTC 1 cut(s) 215
ScrFI CCNGG 2 cut(s) 196, 270
SduI GDGCHC 1 cut(s) 233
SetI ASST 3 cut(s) 206, 233, 239
SfcI CTRYAG 1 cut(s) 13
Sfr274I CTCGAG 2 cut(s) 223, 232
SlaI CTCGAG 2 cut(s) 223, 232
SmiMI CAYNNNNRTG 2 cut(s) 86, 182
SmlI CTYRAG 2 cut(s) 223, 232
SmoI CTYRAG 2 cut(s) 223, 232
SsiI CCGC 4 cut(s) 54, 105, 109, 182
SstI GAGCTC 1 cut(s) 233
StyD4I CCNGG 2 cut(s) 194, 268
StyI CCWWGG 1 cut(s) 128
TaiI ACGT 1 cut(s) 206
TaqI TCGA 3 cut(s) 26, 224, 233
TseI GCWGC 1 cut(s) 12
XhoI CTCGAG 2 cut(s) 223, 232
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.